SMED30035074

Overview
NameSMED30035074
Smed IDSMED30035074
Length (bp)860
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30035074 (SMED30035074) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30035074 (SMED30035074) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30035074 (SMED30035074) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30035074 (SMED30035074) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035074

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30035074h1SMcG0002664 SmedASXL_018818SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30035074h1SMcG0015972 dd_Smed_v4_1190_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30035074h1SMcG0015972 dd_Smed_v4_1190_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30035074h1SMcG0015972 dd_Smed_v4_1190_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30035074h1SMcG0015972 dd_Smed_v4_1190_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30035074 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000015:106205..107223 -4044
Homology
[Close]
BLAST of SMED30035074 vs. Ensembl Human
Match: C1QBP (complement C1q binding protein [Source:HGNC Symbol;Acc:HGNC:1243])

HSP 1 Score: 48.1358 bits (113), Expect = 6.811e-6
Identity = 59/202 (29.21%), Postives = 86/202 (42.57%), Query Frame = 3 
Query:  213 DILKGEITYE---QKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSVD--FD------------QTNPEKDILKSYPDFTITMDRNSGSN--VTFHCTLTNDNEQDEELADTENSIYRVESI--------EVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKS 737
            D L  EI  E   QK   + K   G+  LE  G+E  L R    G K+ VT ++  S+   FD            +  PE   L S P+F + + +N      +   C    D    E+ A  E+ I+ +  +        E  D  NY + +D+LD ++   +MD L +RG D  F  E     T  EH  Y+ FLE +KS
Sbjct:   84 DFLSDEIKEERKIQKHKTLPKMSGGWE-LELNGTEAKLVRKVA-GEKITVTFNINNSIPPTFDGEEEPSQGQKVEEQEPE---LTSTPNFVVEVIKNDDGKKALVLDCHYPEDEVGQEDEA--ESDIFSIREVSFQSTGESEWKDT-NYTLNTDSLDWALYDHLMDFLADRGVDNTFADELVELSTALEHQEYITFLEDLKS 277
[Close]
BLAST of SMED30035074 vs. Ensembl Fly
Match: P32 (gene:FBgn0034259 transcript:FBtr0086892)

HSP 1 Score: 50.0618 bits (118), Expect = 6.586e-7
Identity = 51/202 (25.25%), Postives = 95/202 (47.03%), Query Frame = 3 
Query:  189 SEVETKLADILKGEITYE---QKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSVDFDQ---TNPEKDI-----LKSYPDFTITMDRNSGSNVTFHCTLTNDNEQDEELADTENSIYRVESIEV----SDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKSVLLG 749
            ++ E +L + L  EI  E   QK   V    +GF+V +  G+++ L +   D  KV+V+ ++  +VD ++    NP  D      ++S P F + + + + S ++F C+      Q+ E     N ++ ++ + +     +   Y V  D LD  +   +++LL E+G   EF ++     T  EH  Y+  LEK+     G
Sbjct:   67 TKCERELVEFLTEEIVAERKVQKGKTVPSTLDGFAV-KLTGADVELTKQ-TDKEKVVVSFNVNHTVDSEEEPEINPNADKPDLGEMRSKPQFEVDIIKGN-STLSFTCSFLQGEAQEGEY----NDVFSIDEMAIFEGEWNDKVYAVAGDVLDGYLYDLLINLLEEKGISQEFAEKLSDLATAHEHTSYIGLLEKLSKFTAG 261
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BLAST of SMED30035074 vs. Ensembl Zebrafish
Match: c1qbp (complement component 1, q subcomponent binding protein [Source:NCBI gene;Acc:334619])

HSP 1 Score: 50.8322 bits (120), Expect = 5.943e-7
Identity = 55/195 (28.21%), Postives = 89/195 (45.64%), Query Frame = 3 
Query:  207 LADILKGEITYE---QKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSV------DFDQTNP----EKDILKSYPDFTITMDRNSGSN-VTFHCTLT------NDNEQDEELADTENSIYRVESIEVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKI 731
             A+ L  EI  E   QK   + K   G+  LE  G+E  L R+   G KV +T ++  S+      + +QT      E DI+ S P+F + + ++   N + F C          D E++ ++       ++ E  E     +Y + +D+LD ++   +MD L +RG D  F  E     T  EH  Y+KFLE +
Sbjct:   72 FAEFLTEEIKEEKKIQKSKSLPKMSGGWE-LELNGTEAKLIRSL-SGEKVTITFNVNNSIPPQLEEEPEQTQKSQEEEPDIV-STPNFVVEVTKSGAKNSLVFDCHFPEDEVGHGDGEEESDIFTIREVSFQPEGEEDWKETSYTLNTDSLDWALYDHLMDFLADRGVDNTFADELMELSTALEHQEYIKFLEDL 263
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BLAST of SMED30035074 vs. Ensembl Mouse
Match: C1qbp (complement component 1, q subcomponent binding protein [Source:MGI Symbol;Acc:MGI:1194505])

HSP 1 Score: 47.3654 bits (111), Expect = 7.499e-6
Identity = 56/205 (27.32%), Postives = 92/205 (44.88%), Query Frame = 3 
Query:  189 SEVETKLADILKGEITYEQK-RVYVSKPP-NGFSVLESKGSEITLERTYPDGVKVLVTADLAGSVD--FD---------QTNPEKDILKSYPDFTITMDRNSGS-NVTFHCTLTNDNEQDEELADTENSIYRVESI--------EVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKS 737
            +E +    + L  EI  E+K + + S P  +G   LE  G+E  L R    G K+ VT ++  S+   FD         +   ++  L S P+F + + +  G   +   C    D    E+  + E+ I+ ++ +        E  D  NY + +D+LD ++   +MD L +RG D  F  E     T  EH  Y+ FLE +KS
Sbjct:   74 TEGDKAFVEFLTDEIKEEKKIQKHKSLPKMSGDWELEVNGTEAKLLRKV-AGEKITVTFNINNSIPPTFDGEEEPSQGQKAEEQEPELTSTPNFVVEVTKTDGKKTLVLDCHYPEDEIGHED--EAESDIFSIKEVSFQATGDSEWRDT-NYTLNTDSLDWALYDHLMDFLADRGVDNTFADELVELSTALEHQEYITFLEDLKS 274
[Close]
BLAST of SMED30035074 vs. TrEMBL
Match: A0A4S2MC67 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_001079 PE=4 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 4.545e-38
Identity = 88/244 (36.07%), Postives = 139/244 (56.97%), Query Frame = 3 
Query:   60 MIRSFLKFSKCFSK--PFSVQCI-NGMNTVIHKTQNN---LSFKKSYYSSEVETKLADILKGEITYEQKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSV--DFDQT-------NPEKDILKSYPDFTITMDRNSGSNVTFHCTLTNDNEQDEELADTENS---IYRVESIEVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKS 737
            M RS L+  +  S   P +++ I    +  + + Q+     S  + +YSS+V+ +  ++L  EI  E++ +Y S PP GFSV +S G+EI L + Y DGV V +  DLAGSV  +FD+          E   L++ PD  I + + +G +V F+C+  +   + +  A +ENS    Y V+S+E+  +P Y+V +D  DD++    M LL ER  D +FQ+E Q FCT+EEH LYLKFL++  +
Sbjct:    1 MSRSVLRALRSLSAAGPSAIRAIPRPTHFTLLRVQSRAPLFSTVQKWYSSQVDRQFTEVLSKEIKQEKENIYDSSPPKGFSVEKSGGTEILLRKEYSDGVCVEIEIDLAGSVNPEFDEAEDELSEKKEEAPTLEARPDIRIRLVKPAGRSVIFNCSFPSGATRQQ--ASSENSNIPTYSVDSVEMERLPGYFVYTDLFDDNMYDHTMQLLMERNLDAKFQEELQDFCTSEEHKLYLKFLDEFHA 242
[Close]
BLAST of SMED30035074 vs. TrEMBL
Match: A0A5K4FBN8 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 6.349e-36
Identity = 77/208 (37.02%), Postives = 125/208 (60.10%), Query Frame = 3 
Query:  150 TQNNLSFKKSYYSSEVETKLADILKGEITYEQKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSV-----DFDQTNPEK----DILKSYPDFTITMDRNSGSNVTFHCTLTNDNEQDEELADTENS---IYRVESIEVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKS 737
            T  +L+    + SS+V+ +L   L  EI  E+   +   PP GF V+++ G +I + + Y DGV V V  +LAGSV     + D + PEK      L+++PD  I + + SG ++ F+C+L   ++ D++L++ E++    Y V+S+EV  IP Y+V +D  DD +    M LL ERG + +FQ+E Q FCT+EEH LYL+FL++ ++
Sbjct:   29 TTKHLTTSTWFSSSQVDRQLNQFLTNEIKQEKANAFFCNPPEGFKVVKTDGCDIVIRKEYQDGVSVDVEINLAGSVSPSIPEDDVSAPEKMEDDAPLEAHPDLRIKLTKPSGRSLVFNCSLPG-HDADKQLSEDESNNLPTYSVDSVEVERIPGYFVYTDLFDDDMYNHTMQLLVERGLNADFQQELQNFCTSEEHKLYLRFLDEFQN 235
[Close]
BLAST of SMED30035074 vs. TrEMBL
Match: A0A504Y8Z1 (Complement component 1 Q subcomponent-binding protein mitochondrial OS=Fasciola gigantica OX=46835 GN=FGIG_09024 PE=4 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 1.713e-34
Identity = 72/196 (36.73%), Postives = 122/196 (62.24%), Query Frame = 3 
Query:  183 YSSEVETKLADILKGEITYEQKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSVDFD-------QTNPEKDI--LKSYPDFTITMDRNSGSNVTFHCTLTNDNEQDEELADTENSI--YRVESIEVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKS 737
            +SS+V+ +  ++L  EI  E++ +Y  +PP GF+V + +G+EI L++ Y DGV V V  +LAGSV+ D        ++ ++D+  L+++PD  I + + SG +V F+C+L + + Q +  A+ + ++  Y V+S+E+  +  Y+V +D  DD++    M LL ER  D  FQ E Q +CT EEH LYLKFL++  +
Sbjct:   47 FSSQVDRQFTEVLSNEIKQEKENMYQCQPPKGFNVTKMEGTEIVLQKQYSDGVLVEVDINLAGSVNPDIEDTDNMPSDKKEDLPTLEAHPDIRIRLTKPSGKSVIFNCSLPSRDTQQQLSAEGDQNLPTYSVDSVEMEGVSGYFVYTDLFDDNMYDHTMQLLMERKLDANFQDELQDYCTAEEHKLYLKFLDEFHA 242
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BLAST of SMED30035074 vs. TrEMBL
Match: A0A430Q506 (Complement component 1 Q subcomponent-binding protein, mitochondrial OS=Schistosoma bovis OX=6184 GN=DC041_0012342 PE=4 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 4.188e-34
Identity = 79/226 (34.96%), Postives = 125/226 (55.31%), Query Frame = 3 
Query:   93 FSKPFSVQCINGMNTVIHKTQNNLSFKKSYYSSEVETKLADILKGEITYEQKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSV-------DFDQTNPEKD--ILKSYPDFTITMDRNSGSNVTFHCTLTNDNEQDEELADTENSI--YRVESIEVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKS 737
             SKP +++ +   +T    +Q  + F   Y  S V+ +L   L  EI  E+   +   PP GF V+++ G E+ + + Y DGV V V  +LAGSV       +   T   +D   L+++PD  I + + SG ++ F+C+L   + + +   D  N +  Y V+S+E+  IP Y+V +D  DD +    M LL ERG D +FQ+E Q FCT+EEH LYLKFL++ ++
Sbjct:   24 LSKPSTIKPLT-TSTWFCSSQGKI-FGCLYECSLVDRQLNQFLTNEIKQEKANAFFCDPPEGFKVVKTNGCEVVIRKEYQDGVFVDVEINLAGSVTPSISEDEMSTTEKSEDDTALEAHPDLRIKLTKPSGRSLVFNCSLPGHDAEKQLSEDESNDLPTYSVDSVEIERIPGYFVYTDLFDDDMYNHTMQLLVERGLDADFQEELQNFCTSEEHKLYLKFLDEFQN 247
[Close]
BLAST of SMED30035074 vs. TrEMBL
Match: A0A0R3VUT3 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS1107 PE=4 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 6.361e-34
Identity = 75/219 (34.25%), Postives = 121/219 (55.25%), Query Frame = 3 
Query:  114 QCINGMNTVIHKTQNNLSFKKSYYSSEVETKLADILKGEITYEQKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSV---DFDQTNPEKD------ILKSYPDFTITMDRNSGSNVTFHCTL-TNDNEQDEELADTEN-SIYRVESIEVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKS 737
             C  G+       Q   + +  +++S+V+ + + IL  EI  E +  +  +PP GF + +  G+ I +++ +PDGV + V  DLAGS+   D +   PE+        L++ P+  I + + SG +V FHC+L ++D E      D+ N   + V+ +EV  IP Y+V +D  DD++   IM LL ERG D +FQ++ Q F T EEH LY  FLE+ K+
Sbjct:   20 HCSYGLRRSRSYIQCVSTLRPRFFASDVDRQFSAILAEEIKQEGENSFACRPPEGFEIDKQDGTTIIMKKAFPDGVVLEVEMDLAGSIAPEDVENEGPEEKNQEEAPPLEARPELKIRLRKPSGRSVLFHCSLPSSDEEATTSEQDSSNFPTFSVDMVEVEKIPGYFVYTDLFDDNMYDHIMRLLTERGVDYDFQRQVQDFATAEEHKLYRSFLEEFKA 238
[Close]
BLAST of SMED30035074 vs. Ensembl Sea Lamprey
Match: c1qbp (complement component 1, q subcomponent binding protein [Source:NCBI gene;Acc:334619])

HSP 1 Score: 48.9062 bits (115), Expect = 2.475e-7
Identity = 52/198 (26.26%), Postives = 87/198 (43.94%), Query Frame = 3 
Query:  207 LADILKGEITYEQK-RVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSVDFDQTNP--------EKDILKSYPDFTITMDR-NSGSNVTFHCTLTNDNEQDEELADTENSIYRVESIEVSDIP---------NYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKSVL 743
              + LK EI  E+  + + + P      +E  G+E  L +    G  + VT ++  S+  +   P        E+  L S P+F + +++ NS  N+ F C   +D     E  D +  I+ +   EVS  P         +Y + +D LD ++   +MD L +RG D  F  E     T  EH  Y+ FLE++   +
Sbjct:   19 FTEFLKDEIKEEKNIQKHKALPKVAGWEIEFNGTEAKLLKKL-QGETITVTFNINNSIPPNADEPAEEKKDESEEPDLVSTPNFVVEVNKSNSKLNLVFDCHYPDDEIAHGEEGDEDGDIFAIR--EVSFQPTAEGEWKDTSYTLNTDTLDWTLYDHMMDFLADRGVDNTFADELVELSTALEHQEYIHFLEELNGFV 213
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BLAST of SMED30035074 vs. Ensembl Nematostella
Match: EDO44585 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVJ1])

HSP 1 Score: 59.6918 bits (143), Expect = 1.550e-10
Identity = 55/207 (26.57%), Postives = 101/207 (48.79%), Query Frame = 3 
Query:  189 SEVETKLADILKGEITYEQKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSVDFDQT---NPEKDILK-----------SYPDFTITMDRNSGSNVTFHCTLTNDN-EQDEELADT--ENSIYRVESIEVSDIPN-------YYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKS 737
            ++ ++ L   LK EI +EQ     + P +     +   + + LER + +G KV++T D+  +V+ DQ+   + E D  +           SYP FT+ + +++GS + F C          +E AD   E  ++++ ++ V D PN       Y   ++N+D ++   ++++L ERG D  F      F T  EH  Y+KFL+ ++ 
Sbjct:   64 TQGDSDLLSFLKDEIKFEQDNSR-NVPKSELFQAKVDDTIVRLEREF-NGEKVVITFDINQNVNEDQSYVSDAESDNERPANEDTAGNIVSYPSFTVDVTKHTGSTLRFTCEYNQGVIPPADEGADNREEEDVFQIINVCVMDSPNSADNKAIYAAETENIDPNLYTMLLNMLAERGVDNSFGHWLLDFSTAIEHQHYIKFLKNLQG 268
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BLAST of SMED30035074 vs. Ensembl Medaka
Match: c1qbp (complement C1q binding protein [Source:NCBI gene;Acc:101171102])

HSP 1 Score: 52.373 bits (124), Expect = 1.667e-7
Identity = 57/209 (27.27%), Postives = 93/209 (44.50%), Query Frame = 3 
Query:  189 SEVETKLADILKGEITYE---QKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSVD-----------FDQTNPEKDILKSYPDFTITMDRNSGSN-VTFHCTLTNDNEQDEELADTENSIYRVESIEVSDIP---------NYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKSVL 743
            +E +    + L  EI  E   QK   + K   G+  LE  G+E  L R +  G KV VT ++  S+              +   E+  + S P+F + + + +G + + F C    D E      + E+ I+ +   EVS  P         +Y + +D+LD ++   +MD L +RG D  F  E     T  EH  Y+KFLE ++S +
Sbjct:   86 TEGDKAFGNFLSDEIKEEKKIQKNKTLPKMSGGWE-LEMNGTEAKLTR-HLSGEKVTVTFNVNNSIPPSFEEEEEAQQQQKPAEEEPEIVSTPNFVVEVTKQNGKHTLVFDCHFPED-EMSHGEGEEESDIFAIR--EVSFQPEGDSEWKETSYTLNTDSLDWALYDHLMDFLADRGIDNTFTDELMELSTAIEHQEYIKFLEDLQSFV 289
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BLAST of SMED30035074 vs. Planmine SMEST
Match: SMESG000068982.1 (SMESG000068982.1)

HSP 1 Score: 479.559 bits (1233), Expect = 8.540e-174
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 3 
Query:   60 MIRSFLKFSKCFSKPFSVQCINGMNTVIHKTQNNLSFKKSYYSSEVETKLADILKGEITYEQKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSVDFDQTNPEKDILKSYPDFTITMDRNSGSNVTFHCTLTNDNEQDEELADTENSIYRVESIEVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKSVLLGKIRF 761
            MIRSFLKFSKCFSKPFSVQCINGMNTVIHKTQNNLSFKKSYYSSEVETKLADILKGEITYEQKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSVDFDQTNPEKDILKSYPDFTITMDRNSGSNVTFHCTLTNDNEQDEELADTENSIYRVESIEVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKSVLLGKIRF
Sbjct:    1 MIRSFLKFSKCFSKPFSVQCINGMNTVIHKTQNNLSFKKSYYSSEVETKLADILKGEITYEQKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSVDFDQTNPEKDILKSYPDFTITMDRNSGSNVTFHCTLTNDNEQDEELADTENSIYRVESIEVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKSVLLGKIRF 234
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BLAST of SMED30035074 vs. Planmine SMEST
Match: SMESG000068982.1 (SMESG000068982.1)

HSP 1 Score: 431.024 bits (1107), Expect = 7.326e-155
Identity = 211/211 (100.00%), Postives = 211/211 (100.00%), Query Frame = 3 
Query:  129 MNTVIHKTQNNLSFKKSYYSSEVETKLADILKGEITYEQKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSVDFDQTNPEKDILKSYPDFTITMDRNSGSNVTFHCTLTNDNEQDEELADTENSIYRVESIEVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKSVLLGKIRF 761
            MNTVIHKTQNNLSFKKSYYSSEVETKLADILKGEITYEQKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSVDFDQTNPEKDILKSYPDFTITMDRNSGSNVTFHCTLTNDNEQDEELADTENSIYRVESIEVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKSVLLGKIRF
Sbjct:    1 MNTVIHKTQNNLSFKKSYYSSEVETKLADILKGEITYEQKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTADLAGSVDFDQTNPEKDILKSYPDFTITMDRNSGSNVTFHCTLTNDNEQDEELADTENSIYRVESIEVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTEFQKEFQAFCTNEEHVLYLKFLEKIKSVLLGKIRF 211
The following BLAST results are available for this feature:
BLAST of SMED30035074 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
C1QBP6.811e-629.21complement C1q binding protein [Source:HGNC Symbol... [more]
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BLAST of SMED30035074 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035074 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
P326.586e-725.25gene:FBgn0034259 transcript:FBtr0086892[more]
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BLAST of SMED30035074 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
c1qbp5.943e-728.21complement component 1, q subcomponent binding pro... [more]
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BLAST of SMED30035074 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035074 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
C1qbp7.499e-627.32complement component 1, q subcomponent binding pro... [more]
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BLAST of SMED30035074 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035074 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2MC674.545e-3836.07Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A5K4FBN86.349e-3637.02Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A504Y8Z11.713e-3436.73Complement component 1 Q subcomponent-binding prot... [more]
A0A430Q5064.188e-3434.96Complement component 1 Q subcomponent-binding prot... [more]
A0A0R3VUT36.361e-3434.25Uncharacterized protein OS=Taenia asiatica OX=6051... [more]
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BLAST of SMED30035074 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035074 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
c1qbp2.475e-726.26complement component 1, q subcomponent binding pro... [more]
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BLAST of SMED30035074 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035074 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO445851.550e-1026.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30035074 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
c1qbp1.667e-727.27complement C1q binding protein [Source:NCBI gene;A... [more]
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BLAST of SMED30035074 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000068982.18.540e-174100.00SMESG000068982.1[more]
SMESG000068982.17.326e-155100.00SMESG000068982.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035074 ID=SMED30035074|Name=SMED30035074|organism=Schmidtea mediterranea sexual|type=transcript|length=860bp
GTTTGTTTTATTACAGCTACTACCATTTTAGTTGATAATTTCAAATTTTG
CTAATAATCATGATTAGATCTTTTCTTAAATTTTCTAAATGTTTTTCAAA
GCCATTTTCTGTTCAATGCATAAATGGCATGAATACAGTTATACATAAAA
CCCAAAACAATTTGTCTTTTAAGAAATCATATTATTCTAGTGAAGTTGAA
ACAAAACTCGCTGACATCTTAAAAGGCGAAATTACTTACGAACAAAAGCG
GGTTTATGTAAGCAAACCACCTAACGGATTTTCTGTTCTTGAGTCTAAAG
GTTCTGAAATTACTCTTGAACGAACTTATCCAGATGGAGTTAAAGTACTT
GTGACAGCAGATTTAGCTGGCTCTGTTGATTTTGATCAAACAAATCCTGA
AAAGGATATCCTAAAATCATATCCTGATTTTACGATCACAATGGATCGAA
ACTCAGGAAGCAATGTTACATTTCACTGCACTTTGACTAATGATAATGAA
CAAGATGAAGAATTGGCAGATACGGAAAATTCTATTTACCGAGTTGAGTC
TATTGAAGTATCAGATATTCCAAATTACTACGTGTTGTCAGATAATTTAG
ATGACAGCGTCAGTCAAGGGATAATGGATTTGTTGTTTGAGAGAGGATTC
GATACAGAATTTCAAAAGGAATTCCAAGCATTTTGCACGAATGAAGAGCA
TGTATTGTATTTGAAGTTTTTGGAGAAAATTAAATCCGTTTTACTCGGTA
AAATAAGATTCTAAGCATTTGTTGACTTGTTGCCTTATTTGATAACTTTT
TATAGTGATTTATTTTATCGATTAGGTAAATTTTAAAGTTTTGTAACAAT
TATAGTAACA
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protein sequence of SMED30035074-orf-1

>SMED30035074-orf-1 ID=SMED30035074-orf-1|Name=SMED30035074-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=235bp
MIRSFLKFSKCFSKPFSVQCINGMNTVIHKTQNNLSFKKSYYSSEVETKL
ADILKGEITYEQKRVYVSKPPNGFSVLESKGSEITLERTYPDGVKVLVTA
DLAGSVDFDQTNPEKDILKSYPDFTITMDRNSGSNVTFHCTLTNDNEQDE
ELADTENSIYRVESIEVSDIPNYYVLSDNLDDSVSQGIMDLLFERGFDTE
FQKEFQAFCTNEEHVLYLKFLEKIKSVLLGKIRF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036561MAM33_sf
IPR003428MAM33
Vocabulary: cellular component
TermDefinition
GO:0005759mitochondrial matrix
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003428Mitochondrial glycoproteinPFAMPF02330MAM33coord: 54..226
e-value: 8.6E-10
score: 38.9
IPR003428Mitochondrial glycoproteinPANTHERPTHR10826COMPLEMENT COMPONENT 1coord: 35..226
IPR036561Mitochondrial glycoprotein superfamilyGENE3DG3DSA:3.10.280.10coord: 42..228
e-value: 5.5E-33
score: 116.2
IPR036561Mitochondrial glycoprotein superfamilySUPERFAMILYSSF54529Mitochondrial glycoprotein MAM33-likecoord: 43..227
NoneNo IPR availablePANTHERPTHR10826:SF1COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIALcoord: 35..226