Sperm associated antigen 17
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30035047 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 16
Homology
BLAST of Sperm associated antigen 17 vs. Ensembl Human
Match: SPAG17 (sperm associated antigen 17 [Source:HGNC Symbol;Acc:HGNC:26620]) HSP 1 Score: 227.639 bits (579), Expect = 8.490e-59 Identity = 223/822 (27.13%), Postives = 368/822 (44.77%), Query Frame = 2
Query: 4172 LLTSYATDPSTGETLVTRSDGTLLLVSQDAGIFLCEHSDGTRFTKYFNVEKSS-------SSTYEPGEISRAKISELLLIESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDGEIIFTASVNSQMSYLQPDGPFS---------YVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQKYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILHSDSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIYSNPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVN-QSDEEKKIADEILEKF----KVLKFSKESIANAIGSLVNINSTQGEMSSKAF-ETNAKTALPKNPSH----RKLDVILEQXXXXXXXXXXXRASLKNHIVPKYFTSIYGKDFISKQAMDLQSLNKSMKLFPISREG---TDVNDLKDKN-EF----LNETETSEV-SLLSPKFQVDYLEQSPENSTKNSKNLLKPKLIIQKRATNYLYYDSNLFIPNEPKRLPELS------------NINLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIANNLEKNSSNVSLLEHFLQITTNTDQLIIPIVGRVMTAENY 6496
LL+ ATDP G + TR D +++ +D G + +H+DGTR T ++ V + +T P ++R + + +ES +Y VI + + G +++ G + + + D ++ +S + Y PF Y+ TS VICE++D EG F V +G + +K LE D + K +S+ SM + N Q Y +H PR F + DGSG ELLR DI + L + + N +E +Q P + T+L + PW+ + T++P L R +TFP E KT GPPFG + KGL I S + V P + L Q RQF + I +V+ L DY IL++ +++ + + +++EE+ A ++L+ K+ + +K + L ++ K +T K K H ++ +++ + E +KN I+P +F S + + S Q L SL+K + F E T V D D N +F ++E ++S V SL P+ D + + +N T+N L K + A +Y EP ++P S + LP+YL + +P +P A V+D V KV T+S+ + + + G ++ P+ V G+LKEG Y +KL N G + FK+KQP STG+ V + PGP+AAG+ +LNI ++A A E + + + H ++I T + L +P+ V+T+ NY
Sbjct: 1417 LLSFQATDPVNGTVMTTREDKVVIVERKD-GTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQ--VKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYY-----PFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKK----------LEDDLN-EKTEGYDSLSSMHLEKNHQ-QIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVK-KEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQL----VSAPGAILKSPSVL---QMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQS-QYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTEN---LTKSP----EEAESY-----------EPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQP---LAKVQDSVGGKVNTSSVASAAINN--AKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVG-EDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNY 2185 HSP 2 Score: 184.111 bits (466), Expect = 1.568e-45 Identity = 130/464 (28.02%), Postives = 232/464 (50.00%), Query Frame = 2
Query: 23 MSKKRNKSPAANIASGNKWESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDF----GNPKSKKGKE-----APLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDM-KNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGSQVPGKRSPEP-PSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYEMQIENLKSFWTNINPVLNDNKT---LLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPV---------KTSVAQTHENTTPRESIKEGVQASPDNTDSHMNFTFDKFNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTP 1345
M+ K+ K N +S WE ++ AA+ N+++W S++ ++ N +D I + + + RK F++++ + I Q IN+ G+ SKK K+ APL +E+ K+ +D E L P + K +K+ +L K +D + + D + K KS + K + K ++ GK PE VK+ +L++RG+ D +YIDDEPDDG+ Y ++ G + LLA +A++G TS++K++ + + P + V Q+ + S+ ++ E I+ LK+FW + PVLN+ K L + + + K FP + + ++ +K ++FE++A LMYD+LD KRQ ++YL+ +LI +P ++ + P K+ P + + ++ YYN+LL + E S+ L + ML+QV T + P
Sbjct: 1 MAPKKEKGGTVNTSS-KIWEPSLIAAQFNQNDWQASIAFVVGNQIEDDLLIQALTVAVQVPQRKLFSMVSWQDILQQINEINTLVGSASSKKAKKPVGGNAPLYYEVLTAAKAIMDSGEKLTLPLIGKLLKFQLLQIKFKDQQRRENEKKVIEDKPKLEKDKGKAKSPKEKKAPSAKPAKGKGKDQPEANAPVKKTTQLKRRGEDDHTNRYIDDEPDDGAQHYIIVVGFNNPQLLAIMAELGIPITSVIKISSENYEPLQTHLAAVNQQQEVLLQSEDLEAEKLKKENAIKELKTFWKYLEPVLNNEKPETNLFDVARLEYMVKAADFPSDWSDGEMMLKLGTDIFENIACLMYDILDWKRQHQHYLESMQLINVPQVVNEKPVLEAMPTSEAPQPAVPAPGKKKAQYEEPQAPPPVTSVITTEVDMRYYNYLLNPIREEFISVPLILHCMLEQVVATEEDLVP 463 HSP 3 Score: 85.1149 bits (209), Expect = 1.810e-15 Identity = 41/119 (34.45%), Postives = 70/119 (58.82%), Query Frame = 2
Query: 2222 LDYLSNLRKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEAIYQANLLNQELQIVETEIK 2578
L+ + ++R+ +W F E ++P VLLQ++ ++ + +D Y + + L+V HNP + + + WN LHSNVGFRNYL+ V I DW +EAIYQ + +N+++ E++
Sbjct: 768 LEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMN-RQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELE 885
BLAST of Sperm associated antigen 17 vs. Ensembl Human
Match: SPAG17 (sperm associated antigen 17 [Source:HGNC Symbol;Acc:HGNC:26620]) HSP 1 Score: 189.504 bits (480), Expect = 3.818e-49 Identity = 185/655 (28.24%), Postives = 299/655 (45.65%), Query Frame = 2
Query: 4610 YVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQKYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILHSDSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIYSNPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVN-QSDEEKKIADEILEKF----KVLKFSKESIANAIGSLVNINSTQGEMSSKAF-ETNAKTALPKNPSH----RKLDVILEQXXXXXXXXXXXRASLKNHIVPKYFTSIYGKDFISKQAMDLQSLNKSMKLFPISREG---TDVNDLKDKN-EF----LNETETSEV-SLLSPKFQVDYLEQSPENSTKNSKNLLKPKLIIQKRATNYLYYDSNLFIPNEPKRLPELS------------NINLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIANNLEKNSSNVSLLEHFLQITTNTDQLIIPIVGRVM 6481
Y+ TS VICE++D EG F V +G + +K LE D + K +S+ SM + N Q Y +H PR F + DGSG ELLR DI + L + + N +E +Q P + T+L + PW+ + T++P L R +TFP E KT GPPFG + KGL I S + V P + L Q RQF + I +V+ L DY IL++ +++ + + +++EE+ A ++L+ K+ + +K + L ++ K +T K K H ++ +++ + E +KN I+P +F S + + S Q L SL+K + F E T V D D N +F ++E ++S V SL P+ D + + +N T+N L K + A +Y EP ++P S + LP+YL + +P +P A V+D V KV T+S+ + + + G ++ P+ V G+LKEG Y +KL N G + FK+KQP STG+ V + PGP+AAG+ +LNI ++A A E + + + H ++I T + L +P+ ++
Sbjct: 51 YIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKK----------LEDDLN-EKTEGYDSLSSMHLEKNHQ-QIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVK-KEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQL----VSAPGAILKSPSVL---QMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQS-QYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTEN---LTKSP----EEAESY-----------EPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQP---LAKVQDSVGGKVNTSSVASAAINNA--KSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVG-EDGAKGSAHISHNIEIMTEHEVLFLPVEANIL 660
BLAST of Sperm associated antigen 17 vs. Ensembl Zebrafish
Match: si:dkey-208b23.5 (si:dkey-208b23.5 [Source:ZFIN;Acc:ZDB-GENE-081104-362]) HSP 1 Score: 182.185 bits (461), Expect = 2.958e-46 Identity = 164/674 (24.33%), Postives = 300/674 (44.51%), Query Frame = 2
Query: 23 MSKKRNKS-------PAANIASGNKWESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEAPLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGSQVPGKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKV--TRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYEMQIENLKSFWTNINPVLND---NKTLLNTPIITLVAKHLMFPVER------ENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPVK----TSVAQTHENT------TPRESIKE----GVQASPDNTDSHMNFTFDKFNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTPIWELNKSDLPKDLNEDSIDVFKKITDNIYFPXXXXXXXXXXXXIPLDDDDENRNKDQNSPIHVLDSITQRDREINTKNFGKNGAL-------GHELQMLVASFRHFDLLK--------------FIPTDDQISRKRAAKLQELINYLNNTGLSAIEINYAIKLFILENLTLSGVDENGVIRINNEDGYFQRNNYQVWDDPY 1885
M KRNKS P A+ WE++++AA E+ W SLS++ +D + I + + +R+ F++++ E + IN+ GNPK++K K+ P+ FE+ E+ K+ +D E + +AK IK+ +L +KNT T + + K + + + GK+ + P +D KL++RG+ + I+YIDDEPDDG +Y +I G + LL+A L +G ++++K+ RD H + + + S+++ L + + + L FW ++ VLN L + + + K + P + EN D ++ +FE +A L+YD LD +RQ+ +YL +LI +P + + Q+ + TPR+ + G++ P++ + ++ YY LL VP E TS+ L + ML+QV + E + D + N D+ + ++ + L D+E + + ++ + +D I+T +N L LQ L FD++K + + + R + QEL+++ + S E+ ++LF+ E+++L+ VDE+G IN + N WD P+
Sbjct: 1 MPPKRNKSSTPSGATPTGAGAANKSWETSLSAAVFEENEWRASLSVVQVEKAEDEQLIGSLVQAVQQPMRRLFSVLSWEDTLEKINELGNPKTRKTKDVPMFFEVTEVAKAVMDAGEEMTVDLIAKLIKFQLLI-----IKNTDVTRRASELKAVEENAHVKVGLNSANKDQGGKGAAKGKKVADIPVPTKDTKLKRRGEEENTIKYIDDEPDDGPQQYILIVGFYEPLLIAVLDSMGIHVSNVIKLGLQRDEH-----SEALEDTEAEEDHQSNEDQEVETLKQKRQQELDVFWKQLDQVLNSENMGSRLSHVARLNYIVKSQLLPQAKCNTEAMENRD-QLAFGAAIFEGVACLVYDSLDWRRQYCHYLSSMRLIQVPAACKSGSPIQQSRPQSSTEVVQTPRKKVASEEDPGIRQEPESP-----VLSTEVDMRYYCDLLNRVPAEITSVPLILHCMLEQVLASEQET----KAGLCDSEERFNRSDQDIMSYMLSSV---------------LSLPQDEEQKKE-------LMKDLGMKDSSISTNQKCRNTLLINHHDERARRLQPLPV-LDDFDVVKTEADIMKQSHEWTSLMSRHTESTTDRLTRAQELLHFCTDEFTSWSELQRLLQLFVFESMSLTTVDESGC--INRSQSH--PPNPTPWDKPF 627 HSP 2 Score: 60.077 bits (144), Expect = 3.555e-8 Identity = 38/126 (30.16%), Postives = 62/126 (49.21%), Query Frame = 2
Query: 2192 QNDEGF-QEILLDYLSNLRKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEAIYQANLLNQELQIVE 2566
+NDE E+ L L R ++ F E + V Q++ + +D + Q R + V+ HNP + W LH++VGFRNYL++V D I+DW + +E +Q + QE ++ E
Sbjct: 649 ENDEQMGNEVTLTDLQETLIRRLEDYNFVEKHSADVFPQVLQSASEMYRCVDTF-QCRDNAVYVICHNPMS-QQRSCKEFWEGSLHTDVGFRNYLEYVADSISDWTKQEEEKWQ---MEQEKKVSE 769
BLAST of Sperm associated antigen 17 vs. Ensembl Zebrafish
Match: si:dkey-208b23.5 (si:dkey-208b23.5 [Source:ZFIN;Acc:ZDB-GENE-081104-362]) HSP 1 Score: 92.8189 bits (229), Expect = 6.734e-19 Identity = 78/317 (24.61%), Postives = 137/317 (43.22%), Query Frame = 2
Query: 4496 IFKHSDNTEIEYRPDGEIIFTASVNSQMSYLQPDGPFSYVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQKYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILHSDSKIKP-WKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIYSNPIMAKNVQKPKKSFRQDHWLNP-------MQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVN-QSDEEKKIADEIL 5419
I+ HS + DG ++++ + + P G YV N ++V+C++ DSE F V +G V N E + ET+ D+ ++N+ + H PRLF +N DGS SELLR D+ D L + D + + + +S + T+L IK W ++P L R ++FP E KT GP FG ++G L+ P + +V + P + RQ P + + R K+ Y +++LQ+ ++ +++ + ++ +EK +E+L
Sbjct: 27 IYPHSGGV-LHIDKDGGAVYSSKASKEQPERDPTG--QYVMNHRADVLCQVTDSEWNHFQVCADGHTAVTTN-------ESEKRKTETETDV----QVNA-------------GFKTHPPRLFVVNEDGSASELLRAGDVEDLLQKAYCDSSIAVLTDPLPDSQESFGITLLRPFPDIKSHWLSSKQDDDIIPTNLKSRKWESFPASEKKTPGPVFGTTLGCSLQFKEKPSKSDSVH-----------VQPVLECPEVLLVRQITQNPPVTESTRRKLQNKVKAYLEQLLQREAQWEKMQLKDPRTAQEKSHQNELL 305
BLAST of Sperm associated antigen 17 vs. Ensembl Xenopus
Match: mstn.1 (myostatin, gene 1 [Source:Xenbase;Acc:XB-GENE-483324]) HSP 1 Score: 197.978 bits (502), Expect = 8.289e-50 Identity = 164/639 (25.67%), Postives = 288/639 (45.07%), Query Frame = 2
Query: 110 EDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEAPLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGSQVPGKRSPE-PPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYEMQI------ENLKSFWTNINPVLNDNKT---LLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALP-VKTSVAQTHENTTPRESIKEGVQASPDN-----TD-----------SHMNFTFD------KFNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTPIWELNKSDLPKDLNE----------DSIDVFKKITDNIY----FPXXXXXXXXXXXXIPLDDDDE-NRNKDQNSPIHVLDSITQRDREINTKNFGKNGALGHELQMLVASFRHFDLLKFIPTDDQISRKRAAKLQELINYLNNTGLSAIEINYAIKLFILENLTLSGVDENGVIRINNEDGYFQRNNYQVWDDP 1882
+D+W S++ +IEN P+D ++ +S LR+ F++++ + + + + GNPK KK KE+PL +E+ E K LD E LP +AK +K+ L K +D++ + K + K+ +K +AKS G + + GK+ PE PP V++ L++RG+ + +YIDDEPDDG+ Y ++ G L+A LA++G + I++++ + +N D + L Q+ ++L++FW + P+LN LL + + K FP + + DL + A EL E++A LMYD LD +RQ + YL +LI +P V + +N P + + +SP TD S M+ + + + +Y LL D+P E S+ + + +L+QV T + P + P L+ DS+ + K N+Y + + L+ D+ Q P LD + Q ++ LL+F + + +R A++ EL++Y + +++A KLF LE+L L+G+D+ G + + + Q++ WDDP
Sbjct: 17 QDDWRVSIAFVIENRPEDEAHTKALSQAVSVPLRRLFSVVSWADMIRQVQELGNPKVKKTKESPLYWEVTEAAKLLLDHGEKLPLSLIAKLLKFQFLNIKQKDLQR------REAEKKAPEDKSKNKPTKAKSPGARSAGKSKGKKGPEAPPPVQKVTSLKRRGEEEETNRYIDDEPDDGAQHYIIVLGFYQPQLVALLAELGISVSCIIRISSQNY------------TSLPANEPDPTVAPEVLEAEQLRKVTLTKSLETFWKYLEPILNSGPPGSRLLEVARLHYMVKERHFPQDWGDSDLLLAYATELDENVACLMYDCLDWRRQHRRYLRNLRLIRVPSVSVGKSPDRQNVPPEAQEADPIPSSPTGRRKTQTDVAPPAASLPPSSPMHMEVEGPSGAAEVDTRFYTDLLSDLPEELQSVPVILHCVLEQVVATTEDLVPPSQAAPKPRPDGLDPNIASHMISVLDSLSLSNKEKKNLYNAFRAQEAEGERAEERRPELLNHHDKIGHRAAQAQPPSALDPVRIE-------------------QEIIGKLPLAQLLRFPQPAAERNSRRLAQIHELMHYCTSDLTPWEGVSWAFKLFTLESLPLTGLDDLGEVEGSGVE--VQQDGDIPWDDP 616 HSP 2 Score: 142.51 bits (358), Expect = 5.189e-33 Identity = 197/832 (23.68%), Postives = 319/832 (38.34%), Query Frame = 2
Query: 3677 EIGRYITSDGRVVTIFKNELIKVLFPDGTVAXXXXXXXXXXXXDDEVEEFKIENINVKESPNSKRKTKQSKSISVEPQTPLNTVLVEDSKPSCNYSRKWT---------ITLSNGEVFETVANLNSIFNPKPLIETIETKLDDEATTDENNDILEANQPTEKERSFKSLQTKERLLTSYATDPSTGETLVTRSDGTLLLVSQDAGIFLCEHSDGTRFTKYFNVEKSS--SSTYEPGEISRAKIS--ELLLIESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDGEIIFTASVNSQM--SYLQPD-GPFSYVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQKYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILHSDSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGL------EIYSNPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVNQSDEEKKIADEILEKFKVLKFSKESIANAIGSLVNINSTQGEMSSKAFETNAKTALPKNPSHRKLDVILEQXXXXXXXXXXXRASLKNHIVPKYFTSIYGKDFISKQAM--DLQSLNKSMKLFP----------ISREGTDVNDLKDKNEFLNETETSE---VSLLSPKFQVDYLEQSPENSTKNSKNLLKPKLIIQKRATNYLYYDSNLFIPNEPKRLPELSNINLPNYLYAERPSIEPNI 6061
E R IT+ G V+ + KVLFPDGT++ S S I ++P + S EP ++SK + RK + + ET +S N KP I T + ++ LE N L++ ATDP G ++TR D + ++ D G + EH+DGTR T ++ + E GE+ I E + +ES + VI++ G +L+ G + S + + DG+ +++ ++ + S Q D P SY+ + T+ VICE++D EG F V ++G V I EE + + D T + + V YD H PR F +N DGSG E+LR R++ D L + D RE P I T+L + PW + ++P L R FPP E GPP G+ V KGL E S+P K PK +Q RQ Y I K+ R L Y + + K+ E++ +++E + + VL F + ++ I SL + + A + + P HR E + +E++ A ++ +P YF S G+ F+ Q D+ L+ + FP G+D + E + +E S+ S +P+ + ++ P +NS+ II R + N + P++ + LP + + +P+ PNI
Sbjct: 1190 EASRVITAQGSVIKYMLDGSTKVLFPDGTISRSPDSGP----------------IVQPQTPPPSSSGEASAHAETEPPPQSLPTTPQESKEAAAEVRKGKSGQKVPVGKMEIPEPSFPETSPLTSSATNVKPAGTWITTTSSGQQIGTRGSERLELNP----------------LMSYMATDPVNGTVMITREDQVVTVLKSD-GTKIVEHADGTRITTFYQETEMPVLRDHMETGEVPPTVIKRVEFVRVESPDFATVILNQEENICCVVFGDGTEVLATPQGTYQVHPSMSGTLTINQDGDAVYSPRTSASILASPRQKDLPPGSYIMSHTAPVICEVLDPEGNLFQVLVDGSTSVHIASGESGEEEEDGEKGLAEAD-------------TREPSPEV--YDLHVPRFFIVNADGSGGEILRNREVEDFLASCYSDPATAVIREPAPELPGIQFITVLKPLPETSPWVVQKETSDLVPPNLISRPWHNFPPIERVRAGPPLGIGVWKGLSIGAKGEARSHPPALKC---PKV----------LQIRQLSCYEPISKEQRERLQLSLKGYIDHVRK-----KEAELMEAANKEPRTENSTDLLKLVLSFPESRESSQISSLDQVQDDIVTLYESAVAPSPEPPAPPAQPHRHGGDWQEIRREIQEQKETLEA-MRGRDIPPYFQSEMGRAFLDTQVSVPDMDLLSSRLPPFPQSQEREEQQESGSSGSDPAADLNHGEGPSSSEESDEPTRSSSAPRRDTEAAKEPPPYVPENSEPNFNWAEIIPPRNYYPMARSLNFDVTGRPRK----GKVKLPTSILSSKPASVPNI 1950 HSP 3 Score: 74.3294 bits (181), Expect = 2.846e-12 Identity = 41/123 (33.33%), Postives = 72/123 (58.54%), Query Frame = 2
Query: 2198 DEGFQEILLDYLSNLRKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEAIYQANLLNQELQIVE 2566
+E Q L D + ++R+ S+WC+ E + +L Q+V + +D Y + + L+V+HNP +P + + SW+ LHSNV FRNYL+ V + I++W R++E YQ + +EL+ ++
Sbjct: 664 EEAAQTQLGD-IRRTQRRSLSDWCYWEHFNSELLKQVVCDAAQPYRCVDYYYHSQDNSLLLVMHNPMNPYHQ-SEESWDMALHSNVSFRNYLELVAESISNWVREEEEKYQEEMAVRELEALK 784 HSP 4 Score: 54.6842 bits (130), Expect = 2.783e-6 Identity = 32/121 (26.45%), Postives = 61/121 (50.41%), Query Frame = 2
Query: 3005 FISYHLDNDLAHFMGMVAHLFPSDGSIIRVFKMQKLLDTTSVRVSVITTQSHVFTIHILDPTYSPDSQIDNFDQILEQFSHSKNICNYGASTCTFNDGITIAASNNGYSGSQLKNKSPIGA 3367
F+ Y++ ++L G HLFP+DG I+ + ++ V+V ++ H F +HI +PT +Q + + + K++ +G+ + T +GI ++ S+ G SG + K P A
Sbjct: 934 FLGYNMGDNLIQVSGGSRHLFPTDGGQIQAEHVHFEKGSSFVKVKLL-KDGHTFLVHITNPTRP--TQEEQEETVWRPNITKKSVSEFGSFSATLENGIQLSLSHYGASGKGPEEKDPALA 1051
BLAST of Sperm associated antigen 17 vs. Ensembl Mouse
Match: Spag17 (sperm associated antigen 17 [Source:MGI Symbol;Acc:MGI:1921612]) HSP 1 Score: 154.066 bits (388), Expect = 1.427e-36 Identity = 129/473 (27.27%), Postives = 230/473 (48.63%), Query Frame = 2
Query: 23 MSKKRNKSPAANIASGNKWESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGN----PKSKKGKEA-----PLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGG-----KKGSQVPGK-RSPEPP----SVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYEMQIENLKSFWTNINPVLNDNKT---LLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPVKTSVAQTHENTTPRESI---------KEGVQASPDNTDSHMNFTF-DKFNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTP 1345
M+ K+ K + A+ WE ++ AA LN+++W S++ ++ N +D I+ ++L + RK F++++ E I Q +++ + +KKGK+ PL++E+ K ++ E L P + K +K +L K++D + + K V+ + KSE+ K G K S PGK +S + P +V++ +L++RG+ D YIDDEPDDG+ Y ++ G + LLA + ++G TS++K++ + + P + V Q+ + ++ + I+ L+ FW + P+LN+ K L + + + K FP + + + + E+FE++A LMYD LD KRQ +YL +LI +P S E T E K+ Q + + F + ++ YYN LL +P E S+ L + M++QV T + P
Sbjct: 1 MAPKKEKPTGS--ANYKIWEPSLIAAHLNQNDWKASIAFVVGNRVEDDLLIHALDLAVRLPQRKLFSIVSWEDILQQMDEIQSLAESASAKKGKKPTSVNLPLHYEVFLAAKIIMESGEKLTLPLIGKLLKCQLLHIKSKDQQRRENEKKMVEERT--------KSEKDKGKGKSPKEKKVASAKPGKGKSKDQPEATVTVRKTTQLKRRGEDDEAKSYIDDEPDDGAQYYIIVVGFNNPQLLAIMTELGIPITSVIKISSENYEPLQTHLAAVRQQQEAVLQPEDIEAEKLKRKNSIKELEVFWKYLEPILNNEKLEIHLFDVARLQYMVKATYFPSDWSDNEQMLALGTEIFENIACLMYDSLDWKRQHHHYLQSMQLINVPQVVSEKTVLEAITIPEPPPSTAPAPTGKKKAQYEESHAPPTVAFIITTEVDMRYYNDLLNPIPEEFISVSLILHCMVEQVVATEEDLIP 463 HSP 2 Score: 133.265 bits (334), Expect = 3.269e-30 Identity = 164/642 (25.55%), Postives = 250/642 (38.94%), Query Frame = 2
Query: 4187 ATDPSTGETLVTRSDGTLLLVSQDAGIFLCEHSDGTRFTKYFNV-------EKSSSSTYEPGEISRAKISELLLIESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDGEIIFTASVNSQMSYLQPDG--------------PFS---------YVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQKYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILHSDSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIYSNP---IMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVNQSDEEKKIADEILEKFKVLKFSKESIANAIGSLVNINSTQGEMSSKAFETNAKTALPKNPSHRKLDVILEQXXXXXXXXXXXRASLKNHIVPKYFTSIYGKDFISKQAMDLQSLNKSMKLFPISREGTDVNDLKDKNEFLNETETSEV-----SLLSPKF--QVDYLEQS----PENSTKNSKNLLKPKLIIQKRATNYLYYDSNLFIPNE 5980
ATDP G + TR D +++ +D G + +H+DGTR T ++ V +T P ++R + + IES Y +I TN + + C + G D T I +P G N+ Y+ D PF Y+ TS VICE+ D EG F V +G + KK LE D ++ ++ S+ S+ + N ++Q Y +H PR F + DGSG ELLR DI + L + + +E +Q P ++ T+L + W + T++P L R + FP E KT GPPFG + KGL I S I A ++ PK +Q RQF + I +V+ L DY IL++ +++ + + EE++ NA L + MS E K+ + K +H L V+ +Q + PK Y K+F+ K+ L + R N++K +L + +T EV S LS F + DYLE+ P KN L+ A LY DS L + E
Sbjct: 1440 ATDPINGTVMTTREDKVIIVEKKD-GTRVVDHADGTRITTFYQVYEDHITPSNDEETTEGPRTVTRQ--VKCMRIESSHYATII---TNCEDSSCCATFG---------------DGTSIIAKPQGSYQVLPP-NTGCLYIDKDCSATYCHESSNNLYHPFQKREQLRASRYIMKHTSEVICEVQDPEGNTFQVMADGSVSTTLPKKK----------LEDDFNV-QMEGYESLSSLHLEKN-HMQIYGEHVPRFFVVYTDGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEYPGALSITVLRPFHEASQWIMKKELDTIVPPNLQSRSWERFPSVEKKTPGPPFGTQIWKGLSIGSKQLTNIPAPILEGPKV----------LQMRQFIQHEVIKNEVKLKLQISLKDYINHILKKEDELQEMTVKDSRTEEER-------------------GNAADLLKLV------MSFPKMEETTKSHMTKVAAH--LTVLFKQSMAS---------------APKCSPDSYSKEFLEKKWRSLSQGTSWKEKLEQQR-----NNIKKTQSYLMQIKTKEVTPYFKSELSSLFKSKYDYLEKFSKSLPPFVKKNEAKLMT--------AVPDLYSDSTLTVDTE 1982 HSP 3 Score: 100.138 bits (248), Expect = 3.093e-20 Identity = 56/163 (34.36%), Postives = 90/163 (55.21%), Query Frame = 2
Query: 6008 INLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIANNLEKNSSNVSLLEHFLQITTNTDQLIIPIVGRVMTAENY 6496
+ LP Y+ + +P +P A VEDPV +V T+S+ + + G ++ P V G+LKEG Y +KL N G + F++KQP STG+ V + PGP+AAGL +L + ++A+A E + + + H ++I T D L +P+ V+T+ NY
Sbjct: 2126 VRLPYYMMSSKPKSQP---YAKVEDPVGGRVNTSSIASAAMYNP--NASPFGFHLLPPSVKFGVLKEGHTYATIVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQAELKVELFAMAVG-EDGAKGSAHISHNIEIMTEHDVLFLPVEATVLTSSNY 2282 HSP 4 Score: 85.5001 bits (210), Expect = 1.016e-15 Identity = 41/112 (36.61%), Postives = 65/112 (58.04%), Query Frame = 2
Query: 2243 RKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEAIYQANLLNQELQIVETEIK 2578
++R+ +W F E+++P VLLQ++ ++ + +D Y + + L+V HNP + H WN LHSNVGFRNYL+ V I DW +EA YQ + +EL + E++
Sbjct: 776 QQRSLMDWSFTEYFQPKVLLQVLQEAHQQYRCVDSYYHTQDNSLLLVFHNPMNLQRLQCEH-WNIALHSNVGFRNYLELVAKSIEDWVTQEEAKYQEAKMAEELNRIRIELE 886
BLAST of Sperm associated antigen 17 vs. UniProt/SwissProt
Match: sp|Q6Q759|SPG17_HUMAN (Sperm-associated antigen 17 OS=Homo sapiens OX=9606 GN=SPAG17 PE=2 SV=1) HSP 1 Score: 227.639 bits (579), Expect = 4.077e-58 Identity = 223/822 (27.13%), Postives = 368/822 (44.77%), Query Frame = 2
Query: 4172 LLTSYATDPSTGETLVTRSDGTLLLVSQDAGIFLCEHSDGTRFTKYFNVEKSS-------SSTYEPGEISRAKISELLLIESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDGEIIFTASVNSQMSYLQPDGPFS---------YVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQKYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILHSDSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIYSNPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVN-QSDEEKKIADEILEKF----KVLKFSKESIANAIGSLVNINSTQGEMSSKAF-ETNAKTALPKNPSH----RKLDVILEQXXXXXXXXXXXRASLKNHIVPKYFTSIYGKDFISKQAMDLQSLNKSMKLFPISREG---TDVNDLKDKN-EF----LNETETSEV-SLLSPKFQVDYLEQSPENSTKNSKNLLKPKLIIQKRATNYLYYDSNLFIPNEPKRLPELS------------NINLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIANNLEKNSSNVSLLEHFLQITTNTDQLIIPIVGRVMTAENY 6496
LL+ ATDP G + TR D +++ +D G + +H+DGTR T ++ V + +T P ++R + + +ES +Y VI + + G +++ G + + + D ++ +S + Y PF Y+ TS VICE++D EG F V +G + +K LE D + K +S+ SM + N Q Y +H PR F + DGSG ELLR DI + L + + N +E +Q P + T+L + PW+ + T++P L R +TFP E KT GPPFG + KGL I S + V P + L Q RQF + I +V+ L DY IL++ +++ + + +++EE+ A ++L+ K+ + +K + L ++ K +T K K H ++ +++ + E +KN I+P +F S + + S Q L SL+K + F E T V D D N +F ++E ++S V SL P+ D + + +N T+N L K + A +Y EP ++P S + LP+YL + +P +P A V+D V KV T+S+ + + + G ++ P+ V G+LKEG Y +KL N G + FK+KQP STG+ V + PGP+AAG+ +LNI ++A A E + + + H ++I T + L +P+ V+T+ NY
Sbjct: 1417 LLSFQATDPVNGTVMTTREDKVVIVERKD-GTRIVDHADGTRITTFYQVYEDQIILPDDQETTEGPRTVTRQ--VKCMRVESSRYATVIANCEDSSCCATFGDGTTIIAKPQGTYQVLPPNTGSLYIDKDCSAVYCHESSSNIYY-----PFQKREQLRAGRYIMRHTSEVICEVLDPEGNTFQVMADGSISTILPEKK----------LEDDLN-EKTEGYDSLSSMHLEKNHQ-QIYGEHVPRFFVMYADGSGMELLRDSDIEEYLSLAYKESNTVVLQEPVQEQPGTLTITVLRPFHEASPWQVK-KEDTIVPPNLRSRSWETFPSVEKKTPGPPFGTQIWKGLCIESKQL----VSAPGAILKSPSVL---QMRQFIQHEVIKNEVKLRLQVSLKDYINYILKKEDELQEMMVKDSRTEEERGNAADLLKLVMSFPKMEETTKSHVTEVAAHLTDLFKQSLATPPKCPPDTFGKDFFEKTWRHTASSKRWKEKIDKTRKEIETTQNYLMDIKNRIIPPFFKSELNQLYQS-QYNHLDSLSKKLPSFTKKNEDANETAVQDTSDLNLDFKPHKVSEQKSSSVPSLPKPEISADKKDFTAQNQTEN---LTKSP----EEAESY-----------EPVKIPTQSLLQDVAGQTRKEKVKLPHYLLSSKPKSQP---LAKVQDSVGGKVNTSSVASAAINN--AKSSLFGFHLLPSSVKFGVLKEGHTYATVVKLKNVGVDFCRFKVKQPPPSTGLKVTYKPGPVAAGMQTELNIELFATAVG-EDGAKGSAHISHNIEIMTEHEVLFLPVEATVLTSSNY 2185 HSP 2 Score: 184.111 bits (466), Expect = 7.528e-45 Identity = 130/464 (28.02%), Postives = 232/464 (50.00%), Query Frame = 2
Query: 23 MSKKRNKSPAANIASGNKWESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDF----GNPKSKKGKE-----APLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDM-KNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGSQVPGKRSPEP-PSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYEMQIENLKSFWTNINPVLNDNKT---LLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPV---------KTSVAQTHENTTPRESIKEGVQASPDNTDSHMNFTFDKFNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTP 1345
M+ K+ K N +S WE ++ AA+ N+++W S++ ++ N +D I + + + RK F++++ + I Q IN+ G+ SKK K+ APL +E+ K+ +D E L P + K +K+ +L K +D + + D + K KS + K + K ++ GK PE VK+ +L++RG+ D +YIDDEPDDG+ Y ++ G + LLA +A++G TS++K++ + + P + V Q+ + S+ ++ E I+ LK+FW + PVLN+ K L + + + K FP + + ++ +K ++FE++A LMYD+LD KRQ ++YL+ +LI +P ++ + P K+ P + + ++ YYN+LL + E S+ L + ML+QV T + P
Sbjct: 1 MAPKKEKGGTVNTSS-KIWEPSLIAAQFNQNDWQASIAFVVGNQIEDDLLIQALTVAVQVPQRKLFSMVSWQDILQQINEINTLVGSASSKKAKKPVGGNAPLYYEVLTAAKAIMDSGEKLTLPLIGKLLKFQLLQIKFKDQQRRENEKKVIEDKPKLEKDKGKAKSPKEKKAPSAKPAKGKGKDQPEANAPVKKTTQLKRRGEDDHTNRYIDDEPDDGAQHYIIVVGFNNPQLLAIMAELGIPITSVIKISSENYEPLQTHLAAVNQQQEVLLQSEDLEAEKLKKENAIKELKTFWKYLEPVLNNEKPETNLFDVARLEYMVKAADFPSDWSDGEMMLKLGTDIFENIACLMYDILDWKRQHQHYLESMQLINVPQVVNEKPVLEAMPTSEAPQPAVPAPGKKKAQYEEPQAPPPVTSVITTEVDMRYYNYLLNPIREEFISVPLILHCMLEQVVATEEDLVP 463 HSP 3 Score: 85.1149 bits (209), Expect = 8.694e-15 Identity = 41/119 (34.45%), Postives = 70/119 (58.82%), Query Frame = 2
Query: 2222 LDYLSNLRKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEAIYQANLLNQELQIVETEIK 2578
L+ + ++R+ +W F E ++P VLLQ++ ++ + +D Y + + L+V HNP + + + WN LHSNVGFRNYL+ V I DW +EAIYQ + +N+++ E++
Sbjct: 768 LEDIKKTQQRSLMDWSFTEHFKPKVLLQVLQEAHKQYRCVDSYYHTQDNSLLLVFHNPMN-RQRLHCEYWNIALHSNVGFRNYLELVAKSIQDWITKEEAIYQESKMNEKIIRTRAELE 885
BLAST of Sperm associated antigen 17 vs. UniProt/SwissProt
Match: sp|Q5S003|SPG17_MOUSE (Sperm-associated antigen 17 OS=Mus musculus OX=10090 GN=Spag17 PE=1 SV=2) HSP 1 Score: 154.066 bits (388), Expect = 9.980e-36 Identity = 129/473 (27.27%), Postives = 230/473 (48.63%), Query Frame = 2
Query: 23 MSKKRNKSPAANIASGNKWESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGN----PKSKKGKEA-----PLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGG-----KKGSQVPGK-RSPEPP----SVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYEMQIENLKSFWTNINPVLNDNKT---LLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPVKTSVAQTHENTTPRESI---------KEGVQASPDNTDSHMNFTF-DKFNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTP 1345
M+ K+ K + A+ WE ++ AA LN+++W S++ ++ N +D I+ ++L + RK F++++ E I Q +++ + +KKGK+ PL++E+ K ++ E L P + K +K +L K++D + + K V+ + KSE+ K G K S PGK +S + P +V++ +L++RG+ D YIDDEPDDG+ Y ++ G + LLA + ++G TS++K++ + + P + V Q+ + ++ + I+ L+ FW + P+LN+ K L + + + K FP + + + + E+FE++A LMYD LD KRQ +YL +LI +P S E T E K+ Q + + F + ++ YYN LL +P E S+ L + M++QV T + P
Sbjct: 1 MAPKKEKPTGS--ANYKIWEPSLIAAHLNQNDWKASIAFVVGNRVEDDLLIHALDLAVRLPQRKLFSIVSWEDILQQMDEIQSLAESASAKKGKKPTSVNLPLHYEVFLAAKIIMESGEKLTLPLIGKLLKCQLLHIKSKDQQRRENEKKMVEERT--------KSEKDKGKGKSPKEKKVASAKPGKGKSKDQPEATVTVRKTTQLKRRGEDDEAKSYIDDEPDDGAQYYIIVVGFNNPQLLAIMTELGIPITSVIKISSENYEPLQTHLAAVRQQQEAVLQPEDIEAEKLKRKNSIKELEVFWKYLEPILNNEKLEIHLFDVARLQYMVKATYFPSDWSDNEQMLALGTEIFENIACLMYDSLDWKRQHHHYLQSMQLINVPQVVSEKTVLEAITIPEPPPSTAPAPTGKKKAQYEESHAPPTVAFIITTEVDMRYYNDLLNPIPEEFISVSLILHCMVEQVVATEEDLIP 463 HSP 2 Score: 133.265 bits (334), Expect = 2.287e-29 Identity = 164/642 (25.55%), Postives = 250/642 (38.94%), Query Frame = 2
Query: 4187 ATDPSTGETLVTRSDGTLLLVSQDAGIFLCEHSDGTRFTKYFNV-------EKSSSSTYEPGEISRAKISELLLIESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDGEIIFTASVNSQMSYLQPDG--------------PFS---------YVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQKYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILHSDSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIYSNP---IMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVNQSDEEKKIADEILEKFKVLKFSKESIANAIGSLVNINSTQGEMSSKAFETNAKTALPKNPSHRKLDVILEQXXXXXXXXXXXRASLKNHIVPKYFTSIYGKDFISKQAMDLQSLNKSMKLFPISREGTDVNDLKDKNEFLNETETSEV-----SLLSPKF--QVDYLEQS----PENSTKNSKNLLKPKLIIQKRATNYLYYDSNLFIPNE 5980
ATDP G + TR D +++ +D G + +H+DGTR T ++ V +T P ++R + + IES Y +I TN + + C + G D T I +P G N+ Y+ D PF Y+ TS VICE+ D EG F V +G + KK LE D ++ ++ S+ S+ + N ++Q Y +H PR F + DGSG ELLR DI + L + + +E +Q P ++ T+L + W + T++P L R + FP E KT GPPFG + KGL I S I A ++ PK +Q RQF + I +V+ L DY IL++ +++ + + EE++ NA L + MS E K+ + K +H L V+ +Q + PK Y K+F+ K+ L + R N++K +L + +T EV S LS F + DYLE+ P KN L+ A LY DS L + E
Sbjct: 1440 ATDPINGTVMTTREDKVIIVEKKD-GTRVVDHADGTRITTFYQVYEDHITPSNDEETTEGPRTVTRQ--VKCMRIESSHYATII---TNCEDSSCCATFG---------------DGTSIIAKPQGSYQVLPP-NTGCLYIDKDCSATYCHESSNNLYHPFQKREQLRASRYIMKHTSEVICEVQDPEGNTFQVMADGSVSTTLPKKK----------LEDDFNV-QMEGYESLSSLHLEKN-HMQIYGEHVPRFFVVYTDGSGVELLRDSDIEEYLSLAYGESTTVVLQEPVQEYPGALSITVLRPFHEASQWIMKKELDTIVPPNLQSRSWERFPSVEKKTPGPPFGTQIWKGLSIGSKQLTNIPAPILEGPKV----------LQMRQFIQHEVIKNEVKLKLQISLKDYINHILKKEDELQEMTVKDSRTEEER-------------------GNAADLLKLV------MSFPKMEETTKSHMTKVAAH--LTVLFKQSMAS---------------APKCSPDSYSKEFLEKKWRSLSQGTSWKEKLEQQR-----NNIKKTQSYLMQIKTKEVTPYFKSELSSLFKSKYDYLEKFSKSLPPFVKKNEAKLMT--------AVPDLYSDSTLTVDTE 1982 HSP 3 Score: 100.138 bits (248), Expect = 2.164e-19 Identity = 56/163 (34.36%), Postives = 90/163 (55.21%), Query Frame = 2
Query: 6008 INLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIANNLEKNSSNVSLLEHFLQITTNTDQLIIPIVGRVMTAENY 6496
+ LP Y+ + +P +P A VEDPV +V T+S+ + + G ++ P V G+LKEG Y +KL N G + F++KQP STG+ V + PGP+AAGL +L + ++A+A E + + + H ++I T D L +P+ V+T+ NY
Sbjct: 2126 VRLPYYMMSSKPKSQP---YAKVEDPVGGRVNTSSIASAAMYNP--NASPFGFHLLPPSVKFGVLKEGHTYATIVKLKNVGVDFCRFRVKQPPPSTGLKVTYKPGPVAAGLQAELKVELFAMAVG-EDGAKGSAHISHNIEIMTEHDVLFLPVEATVLTSSNY 2282 HSP 4 Score: 85.5001 bits (210), Expect = 7.104e-15 Identity = 41/112 (36.61%), Postives = 65/112 (58.04%), Query Frame = 2
Query: 2243 RKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEAIYQANLLNQELQIVETEIK 2578
++R+ +W F E+++P VLLQ++ ++ + +D Y + + L+V HNP + H WN LHSNVGFRNYL+ V I DW +EA YQ + +EL + E++
Sbjct: 776 QQRSLMDWSFTEYFQPKVLLQVLQEAHQQYRCVDSYYHTQDNSLLLVFHNPMNLQRLQCEH-WNIALHSNVGFRNYLELVAKSIEDWVTQEEAKYQEAKMAEELNRIRIELE 886
BLAST of Sperm associated antigen 17 vs. TrEMBL
Match: R7TXJ7 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_206219 PE=4 SV=1) HSP 1 Score: 528.094 bits (1359), Expect = 1.581e-149 Identity = 548/2041 (26.85%), Postives = 906/2041 (44.39%), Query Frame = 2
Query: 104 LNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEAPLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGSQVPGKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKV-----TRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYEMQIENLKSFWTNINPVL---NDNKTLLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALP--------VKTSVAQTHENTTPRESIKEG-VQASPDNTDSHMNFTFDKFNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTPIWELNKSDLPKDLNEDSID--VFKKITDNIYFPXXXXXXXXXXXXIPLDDDDENRNKDQ----NSPIHVL---------DSITQRDREINTKNFGKNGALGHELQMLVASFRHFDLLKFIPTDDQISRKRAAKLQELINYLNNTGLSAIEINYAIKLFILENLTLSGVDENGVIRINNEDGYFQRNNYQVWDDPYMILNDVAQSIIDEENKTLKXXXXXXXXXXXXXXXXXPALSFIKNENFTEGILKXXXXXXXXXXPENIKSRRKSSVHF---LETERVESP----------------TMSTETQMDIMAFINNQNDEGFQEILLDYLSNLRKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEAIYQANLLNQEL-QIV-ETEIKETEVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTQELSLIVPGSLKAQKLXXXXXXXXXXXXXXXXXXXRQLSAQK-KTEKDLAKNVEKTKDSKRPXXXXXXXXXXXXXXFEDSGENFDKENYQEIYTDRIDGEEYWPFISYHLDNDLAHFMGMVAHLFPSDGSIIRVFKMQKLLDTTSVRVSVITTQSHVFTIHILDP-----------------------TYSPDSQIDNFDQIL------EQFSHSKNICNYGASTCTFNDGITIAASNNGYSGSQLKNKS----PIGAPNDEISSHRSNDLTVNGNKEEIFQNDTNETNEKIITQKNPFVNHMDYIDSPN------------IILTTPDGVQVSVGYESA-----ENENQRNLLLFMCKFESSLKPSVPPELSSIQA---------PEIGRYITSDGRVVTIFKNELIKVLFPDGTVAXXXXXXXXXXXXDDEVEEFKIENINVKESPNSKRKTKQSKSISVEPQTPLNTVLVEDSKPSCNYSRKWTITLSNGEVFETVANLNSIFNPKPLIETIETKLDDEATTDENNDILEANQPTEKERSFKSLQTKERLLTSYATDPSTGETLVTRSDGTLLLVSQDAGIFLCEHSDGTRF-TKYFNVEKSSSSTYEPGEISRAKISELLLI--ESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDGEIIFTASVNSQMSYLQPDGPFSYVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQK----YDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTIL--HSDSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIYSNPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKL---IDYNKKILQQRQLYKDLEIVN-QSDEEKKIADEILEKFKVLKFSKESIANAIGSLVNINSTQGEMSSKAFETNAKTALPKNPSHRKLDVILEQXXXXXXXXXXXRASLKNHIVPKYFTSIYGKDFISKQAMDLQSLNKSMKLFPISREGTDVNDLKDKNEFLNETETSEVSLLSPKFQ 5848
L +D W+ S++ +I + +D E ++++ I+ G R+ F++ T +++ +++ND GNPK+KK KE P EICE K HLD E +P P LAK IK+ +L K +D+K + K KKA ER KS G KG + +P+PPS + D KL+KRG+ D D +YIDDEPDDG Y +I G LL+ LAD+ SI++ R I P + E ++++ ++ E + + E + + FW +I ++ + L + + + K + P E+ LK + ++++E +A +MYDL+D KRQW+NYL+ L+ +P T + TP ++ G S DS + ++ +Y L+ VP ES+SI L M ML+QV T ++ P S+ P D +D V I+ + K+ L +D++ Q + P ++ D IT+R + K A+ EL ML DL KF +Q++++RAA LQEL+++ GL+ +EI +A+ F+LE + LS +E+ ++ D F + N WD P+ + + DE+ L + + S + GILK S++P + SVHF E V P + S T D M+ +N++ +KR +WCF E ++P +LLQ+++Q++ PY+D Y RR + LVVLHNP H + W LHSNVGFRNY+D V I +W R +E+ YQA +L +E+ QI+ + E + ++ K + +S SS+ E T I GSLKA K +Q+K+ + E+E +K++KR SA+K K+ KD+ + +++K KRP S+ S+KS+ K D+ E+ D E +WPFI Y + N+L H G + LFP+DG I+ K + + T SV+ +V+ HVFT+HILDP T D N ++ + E+ + + ++G+ + F+DG+ ++ S G SG + P AP S + D + +K + D T + +++ + + +++P+ + L+ PDG+ ++ ES+ ++E+ R+LL ++ S P + IQA E+ R I +DG V + I V+F DG V+ S + + +E+P K+ +++S V + P VE+ + + W TL +G E VA+ ++ P+ + ATDP TG+T+ TR D + + D G + EH+DGTR T +E + E GE ++ L LI E Y V D T G+ + ++ G + H D + DG ++ +++ P Y+ +S+VICE +D+ G F V+ GE V + + D S +ET+ +D +QK Y QHAP F I+ DGSG+ELLRY+D+ + +++ D + + + + +I T+L + + + W Y T++P GL RD K+ PPKE+ GP FG +VG+GL I S K + + +Q RQ Y + ++R+ L ++ KK L+Q++L L++++ +SDEE+ A+++ + V ++ + + + +A ++A EQ+K +E E + +LK H VPKYF S GK F+ QA D++SL K +L +R G +N +++T + +SP Q
Sbjct: 87 LKKDKWNASVAFVIGHQAEDYEHLSHLVAAINAGTRRLFSIFTKDELYEMVNDLGNPKAKKAKEIPQFSEICEPCKMHLDNGEDIPMPLLAKLIKFRLLDIKQKDLKRRETEKRIGGDKDKGGKKADKGKERTKSPGKGKGGKK----TPDPPSARGDTKLKKRGEEDIDNKYIDDEPDDGPQHYIMICGFHQPRLLSHLADLSICVDSIIRFKSEDYARFIPDPTDEDLEMDKDEKMLA----LEEVRSQVKEKLSKEIAQFWKDILLIMRAASPASPLHDVAQLEYIVKESIIPPNMEDNGLKTQFGVDMYEDIACMMYDLMDAKRQWRNYLESLHLLPVPEYGLPPPSPDTKDGTSQPAVTPAPTMMTGKADNSSQPGDSCEPKLSHEVDMRFYQDLMNTVPQESSSIPLIMHCMLEQVVATEEDREP-----PSETPHPTRADGLDDTVASHISSMAF-------------KLALSEDEQQALSQQLDTVSCPAPLMKQPLLLSYHDDITRRLNHL--KPVDDFDAMKAELSML-GFLPMADLAKFKQPSEQLAKERAASLQELLHFCITGGLTQLEIEHAMCQFVLECVELSETNEDSIVCQRPRD--FPQYNIP-WDHPFPFFQGMILA-CDEDAIPLDESS-----------------SSTRKSRPGSGILK------SSRTPSATSRKPIHSVHFERDGEGNEVVQPGDDKTPADTPSNYDHGSGSVPTNTDPMSVVNDK---------------AQKRVLDQWCFAEHHKPKILLQVMNQALSTHPYMDTYYHRRDNSLLVVLHNP-HSSELQSFTKWEHSLHSNVGFRNYMDHVAGSIEEWTRAEESKYQAAMLAKEVDQIMNDAEEASSPPTSGKGGKKKERSKSPKKSTSRAKSSSRSSSVERPTSGNMYIRQGSLKAWKEDQDKVRAEEDEKERQKQEKRAKSAEKRKSAKDIGDDDKESK--KRPGSRQSSKSRQSKEDIVQ-----DQTLSDEVAPD----EPFWPFIGYDVGNNLIHASGSLTTLFPADGGQIKTEKTEFIQGTLSVKTNVL-KDGHVFTVHILDPKKGGEDEEAKEEEEEAMEKGELETQEKDESDKNGERKVVAEKQEEKKAEKPCVGHFGSFSALFSDGLNVSLSTYGASGMPINGSKYEPVPYIAPAVRSPSPQGKDGSPTKHKRDK-SKDKAATPDPSAQRESELLAKEEEVEAPSEPEPVPHQPFQQLFLSCPDGLNLTYFLESSLGIHPKSEDSRHLL---------VRQSYPYKTKGIQACEGARKNAMTEVSRIINADGTVTKTNTDGTITVMFADGAVSHYCTSSALLPGSRNASPTRVGSAKSDRETPTRKKGSRRSGKKEVVLEQP-----VEEDRADKAF---WITTLPSG---ERVASQGRTYSKVPV---------------------------------------KMAKICVATDPQTGQTMHTRDDHVVTIYMPD-GSMVVEHADGTRINTLQHQLELKVEAEGETGEDGETRVKNLKLISVECPGYATVKFDSDAGHCSTELGNGDCVYTSLDGTYDLHHHDGGCLHMETDGTSVYFPMPANEVDMPNPTQQLQYIMRHSSDVICETVDNMGNTFTVSNAGEGSVVLAQGQD------SVAMETE----------------GGESDLIQKQVVTYKQHAPHFFIIHKDGSGTELLRYQDVASYVSDAESDPSTAILMDPLPDHMGVIGITVLKPYMNGISESWLKNYEMDTIIPAGLISRDFKSVPPKEVVKPGPKFGTNVGQGLAIGS-------AMKGQPVLPMLKCPSVLQLRQILQYKPVTSELRTKLGTSLKMYAEFVKKRLEQQEL---LQVIDPRSDEERSAAEQLQGQVLVPGLAQGDVKGLYEKVTAPPVPSPPPNPQAKRSSAD---------------WEQDKRILAEEKECKRALKQHDVPKYFDSEIGKSFLLSQAPDMESLTK--QLADDTRRG--------RNPVISQTSSESSPQISPDQQ 1925 HSP 2 Score: 113.235 bits (282), Expect = 6.750e-21 Identity = 60/125 (48.00%), Postives = 77/125 (61.60%), Query Frame = 2
Query: 6002 SNINLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIA 6376
S LP Y+ AERP PN VEDPVRRKV T SL G + + L + RG ++ P ++ G LKEG Y + L NTG ++ FK+KQP STG+ V + GPIAAGL+R+L + IYAIA
Sbjct: 2065 SGTLLPGYMRAERPGAVPNDKYIEVEDPVRRKVNTISLTGATPHGKHLLKHMRGCHLLPEAINFGWLKEGNTYAMSVLLKNTGVDTCRFKVKQPPPSTGLNVIYKLGPIAAGLNRELIVEIYAIA 2189
BLAST of Sperm associated antigen 17 vs. TrEMBL
Match: V3ZPH0 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_232733 PE=4 SV=1) HSP 1 Score: 414.075 bits (1063), Expect = 6.958e-113 Identity = 453/1684 (26.90%), Postives = 760/1684 (45.13%), Query Frame = 2
Query: 23 MSK--KRNKSPAANIASGNKWESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEAPLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGSQVPGKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKV-TRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLY----EMQIEN-LKSFWTNINPVL---NDNKTLLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPVKTSVAQTHENTTPRESIKE----------GVQASPDNTDSHMNFTFDKFNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTPIWELNKSDLPKDLNEDSIDVFKKITDNIYFPXXXXXXXXXXXXIPLDDDDENRNKDQNSPIHVLDSITQRDREINTKNFGKNGALG-HELQMLVASFRHF-DLLKFIPTDDQISRKRAAKLQELINYLNNTGLSAIEINYAIKLFILENLTLSGVDENGVIRINNEDGYFQRNNYQVWDDPYMILND-------------------------VAQSIIDEENKTLKXXXXXXXXXXXXXXXXXPALSFIKNENFTEGILKXXXXXXXXXXPENIKSRRKSSVHFLETERVESPTMSTETQMDIMAFINNQNDEGFQEILLDYLSNL---RKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEAIYQANLLNQELQIVETEIKETEVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTQELS-LIVPGSLKAQKLXXXXXXXXXXXXXXXXXXXRQLSAQKKTEKDLAKNVEKTKDSKRPXXXXXXXXXXXXXXFEDSGENFDKENYQEIYTDRIDGEEYWPFISYHLDNDLAHFMGMVAHLFPSDGSIIRVFKMQKLLDTTSVRVSVITTQSHVFTIHILDP----------------TYSPDSQIDNFDQILEQFSHSKNIC--------NYGASTCTFNDGITIAASNNGYSGSQLKNK--------------SPIGAPNDEISSHRSNDLTV--------------NGNKEEIFQNDTNETNEKIITQKNPFVNHMDYIDSPNIILTTPDGVQVSVGYES-----AENENQRNLLLFMCKFESSLKPSVPPELSSIQA----------PEIGRYITSDGRVVTIFKNELIKVLFPDGTVAXXXXXXXXXXXXDDEVEEFKIENINVKESPNSK--RKTKQSKSISVEPQTPLNTVLVEDSKPSCNYSRKWTITLSNGEVFETVANLNSIFNPKPLIETIETKLDDEATTDENNDILEANQPTEKERSFKSLQTKERLLTSYATDPSTGETLVTRSDGTLLLVSQDAGIFLCEHSDGTRFTKYFNVEKSS-SSTYEPGE--ISRAKISELLLIESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDGEIIFTASVNSQMSYLQPDGPFSYVFNPTSNVICEIIDSEGTEFIVNINGEFDV 4702
MSK KR KS A G+KW+ + + L E+NW +++ ++ N P+D I + + G RK F+LI+ + + Q + D GNPK KK KE P NFEICE K HLD + +P P LAK IK+ +L K +D+K + KK + + + + K ++ GK S+ GK++PEPPS K+ +KLRKRG+ D + +++DDEPDDG+ Y +I G L L+++G I+++ ++D I Q N D++ ++ L Q++ LK FW +I VL +DN L + I+ K ++FP E+ + K+ LFE +A +YDL+D +R + YL+ KL +PV V Q H +S V A+ DS ++ YY+ L+ VP ES S+ L + ML+QV T K P E LN + + ++ L ++ +P N+P L + + + +L +++ + F F +++K ++++RAA+LQELI++ N ++ EI+ A K F+ E + L+ D NG I +G ++ WDDPY + ++ E ++ + D++S +S P+ S K +GILK SRSQSP + R +SVHF++ E P + E + F + ++ ++ + D + + +KRN E+C E+YEP+VLLQ++ + Y LP+++ Y +R ++VLH+P H +H W+ LHSN+GFRNY+++V + I +W ++++A Y A +L++E++ ++ E + + + K + KK ++++S TSR SS E+ ++++ S I GSLKAQK + + + ++E+K +LS K+++ K ++ K+ KRP S+ S KSKT E E+ + ++E E+YWPF + + N+L H G+ LFPSDG IRV + + + TTS+ +V+ H+F++HIL+P + + + + EQ K+ ++G+ T T NDG+T+A S G +G + K +P+ P + +G+KEE ++D N +N+ + LT PDG+ V ES A++ R LL +K S P + IQA E R I SDG VV + ++VL+ DGT++ + S++ + +SP K R SK++S + Q V++ + C +W T NGE + +V ++ + KP ++ A+DP T +++ TR D ++ VS G + EHSDGTR T Y+ E GE IS + ++ + +E Y V + + + T G+ ++ G ++ +H D I+ +G + + A ++ LQ + YV ++VICE +D+EG F V G+F V
Sbjct: 1 MSKAGKRVKSGTAP-QGGSKWDIALQSTPLEEENWKSNITFVVGNSPEDYSHIKLLGQAVLGGTRKLFSLISKDDLIQEVQDLGNPKVKKTKENPQNFEICEACKLHLDLGDEIPLPLLAKLIKYRLLNIKQKDLKRR-ELEKKANAPEAASGQDKGKKDKGGKDRGKSPSKKGGKKTPEPPSAKEGSKLRKRGEEDVESKFLDDEPDDGAQHYVIIYGFHQPHLFTHLSEVGINVDCILRISSQDYSI---------FQQPSTENNPDEKAANAELILKEERKQLKKELKKFWKDILLVLQKNSDNSKLHDIAILDYEIKTILFPSNIEDPEQKLNFCTTLFEDVACKIYDLIDSRRLYHTYLENLKLSHVPV---VGQPHIQHIDGQSTASAATPAPGGAAPVSATALPPDSATEAP--SVDMRYYSDLMNCVPQESASVPLILHCMLEQVVATEENKEPPSEQVPPQRSDGLNPQLASYLSSMAFKLALSEDEHKSLGETMDLP--------EIPPNAPPQPLLMNYHDENTVRCHHLKPQYSLDPKQVEEDMLQFSPFSEVVKLERPTTPVAKERAARLQELIHFCANEMMTQAEIDRAFKQFLFECMDLTTTDSNGFIMTLEGEGL--EHSAIPWDDPYPFFKGMIPHHEKHLDIQVPSESSASSYEEKINNDMVVPETRSSRVGFVDDIIDNVSQTKSRPSSSDSK-----KGILK---PRSRSQSPTHGGRSRSNSVHFMKEEGEPLPLNADE----LKEFEGIKEEDIKEKTIEDSMMEVVDAQKRNLDEFCISEYYEPNVLLQVLRDAAYYLPFVNTYYNKRDHSLMLVLHSP-HNSELQSHVDWHTELHSNMGFRNYIEYVQESIGEWLKEKQAEYDAKMLSKEVEKLQFEDEAAALLAEKMKQPDSPKKSSRARTKSPKTSRNSSVEKLASEKSSQFIRAGSLKAQK-------EEADKIKAEEEEKERLSQMKRSKSSQKKEEKEEKEKKRPGSRGSAKSKTSTKEIEPQQESIPLDLHEE--------EKYWPFTGFDVGNNLIHVSGITTSLFPSDGGQIRVERTEFVQGTTSINSTVL-KDGHIFSVHILEPKEDEIISEDEEEEQQLDKDEEEEKKDLPEKCEQEKFEKDGAEGKKVVKSSFGSVTATLNDGMTLAVSQFGSTGEVPEGKKYEPQPYMPPVSSPTPVSQPASPGKGKKGEKTKEKGVPTPEPPQIQAEDGDKEEDTKDDQNSSNQSFQ----------------QLFLTCPDGLHVRYFLESSIGIKADSVLDRRLL---------VKQSYPYKTKGIQACESRRKKYMLSEQSRVIASDGTVVKTMVDGTVEVLYADGTISSRVGKWPLASSRNSSPQR---NTSGRSDSPTKKNVRSKGSSKNLSAKKQ---EEAPVDEEEKKC----RWITTYPNGEKY-SVRTDGTVEDLKP------------------------------------------VMICLASDPETRQSMATRDD-KVVTVSYPDGTTIVEHSDGTRITTYYCESAVPLDDEDETGETPISDVRTTKFVKVECPAYATVEFNCSTSENLTIFGNNTSVNVFPDGFYMIQHIDGGRIQVDTEGTVTYYARLDRPKDNLQRE--LQYVLRHNADVICETVDTEGNIFNVKHTGDFSV 1548 HSP 2 Score: 134.806 bits (338), Expect = 1.850e-27 Identity = 67/169 (39.64%), Postives = 106/169 (62.72%), Query Frame = 2
Query: 5993 PELSNINLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIANNLEKNSSNVSLLEHFLQITTNTDQLIIPIVGRVMTAENYQ 6499
P +++LP + RP+ E NI +E+PVRRKV T + G +E + RGLN+ P +V+ G+L+EG Y++ + L NTG ++ +KIKQP +TG+ V + PGP+AAG+ +L + ++AIA + S V +EH LQITT TD+L +P+ ++TA+ Y+
Sbjct: 1993 PRSDSVSLPESIMGARPNAEKNIKFLEIEEPVRRKVKNTMITGATEKGRAELEDMRGLNILPDKVNFGVLREGYTYSYSVYLKNTGVDTCRYKIKQPPPATGLKVIYKPGPVAAGMRAELRLELFAIAVG-VEGESGVGGIEHNLQITTETDKLYLPVRATILTAQEYE 2160 HSP 3 Score: 67.0106 bits (162), Expect = 8.164e-7 Identity = 64/259 (24.71%), Postives = 110/259 (42.47%), Query Frame = 2
Query: 4961 CREEIQNSPKIIAHTILHS--DSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIYSNPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVN-QSDEEKKIADEILEKFKVLKFSKESIANAIGSLVNI--------NSTQGEMSSKAFETNAKTALPKNPSHRKLDVILEQXXXXXXXXXXXRASLKNHIVPKYFTSIYGKDFISKQA 5704
C++ I P + +L + W +Y +++P G+ RD+ PPKE K GPPFG +VGKGL I S QK + + ++ RQ Y I +R L Y + ++ + + + +++ + +S +E+ +A ++ +S+A +GS +T E+ A + P RK E+++ E+E RA L+N +PKYF S G F Q+
Sbjct: 1558 CKDPITECPGVFGINVLKPFLGGPSQQWFKDYDLDSIIPNGIRSRDLTNLPPKEYKKPGPPFGTNVGKGLAIGS-------TQKTSRRVAILKCPDILEIRQLVQYEPITDPLRIKLCNGLRSYAEYVMARNKESEMMQVNDPRSKKEQMLAHDL----------NKSVARQLGSGCEELQEQPSYDPTTVKEIYESATTAPEPSPPPTPMPKRKAS-DWERDQKEIALELEGRAMLRNKTIPKYFDSELGMAFFQLQS 1798
BLAST of Sperm associated antigen 17 vs. TrEMBL
Match: A0A430QR50 (Uncharacterized protein OS=Schistosoma bovis OX=6184 GN=DC041_0006870 PE=4 SV=1) HSP 1 Score: 333.183 bits (853), Expect = 7.716e-88 Identity = 433/1671 (25.91%), Postives = 700/1671 (41.89%), Query Frame = 2
Query: 2231 LSNLRKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSN-VGFRNYLDFVHDKITDWNRDQEAIYQANLLNQE-LQIVETEIKETEVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTQEL---------SLIVPGSLKAQKLXXXXXXXXXXXXXXXXXXXRQLSAQKKTEKDLAKNVEKTKDSKRPXXXXXXXXXXXXXXFEDSGENFDKENYQEIYTDRIDGEEYWPFISYHLDNDLAHFMGMVAHLFPSDGSIIRVFKMQKLLDTTSVRVSVITTQSHVFTIHILDPTYSPDSQIDNFDQILEQFSHSKNICN----------YGASTCTFNDGITIAASNNGYSGSQLKNKSPIGAPNDEISSHRSNDLTVNGNKE-----EIFQNDTNETNEKIITQKNPFVNHMDYIDSPNIILTTPDGVQVSVGY-----------ESAE------------------NENQRNLLL-----FMCKFESSLKPSVPPELS-------SIQAPEIGRYITSDGRVVTIF-------KNELIKVLFPDG-------TVAXXXXXXXXXXXXDDEVEEFKIENINVKESP---------------------------NSKRKTKQSKSISVEPQTPLNTVLVEDSKPSCNYSRK-----WTITLSNGEVF-----ETVANLNSIFNPKPLIETIETKLDDEATTDENNDILE-ANQPTEKERSFKSLQTKERLLTSYATDPSTGETLVTRSDGTLLLVSQDAGI---FLCEHSDGTRFTK-YFNVEKSSSSTYEPGEISRAKISELLL-IESKQYPAVIIDLTNEKIETRCGSMGALLSN----SCGHHIFKHSDNTEIEYRPDGEIIFTA------SVNSQMSY----------LQPDGPFSYVFNPTSNVICEIIDSEGTEFIVN------INGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTND-SNDNVQKYDQHAPRLFTINP-DGSGSELLRY----------------RDIVDTLV--------------NSDIDENYF---HCREEIQNSPKIIAHTILHSDSKIKPWKYEYSRKTVLPKGLTCRDMKTF-------PPKEIKTDGPPFGMSVGKGLEIY------------SNPIMAKNVQKPKKSFRQDHWLNP------MQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQ-----RQLYKDLEIVNQSDEEKKIADEILEKFKVLKFSKESIANAIGS--LVNINSTQGEMSSKAFETNAKTALPKNPSHRKLDVILEQXXXXXXXXXXXRASLKNHIVPKYFTSIYGKDFISKQAMDLQSLNKSMKLFPISREGTDVNDLKDKNEFLNETETSEVSLLSPKFQVDYL-------EQSPENSTKNSKN--LLKPKLI----------IQKRATN---YLYYD-----------SNLFIPNEPKRLPELSNINLPNYLYAERPSIEP--------------NIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIY-AIANNLEKNSSNVSLLEHFLQITTNTD 6448
L+ + + +EW EE EP+VLLQ +H Y+ PY+D+Y + D ++ H+PF + NH++W W H N +GFR YL + I +W R+QEAIY+A L+ E LQ + TE K K +KS + GS +EE+ Q I+PGSLKA + +QE+ K EE +KE KR SA+K TE++++ ++ KR S K ++S + Q++ T R E +WPF Y + N L H G V H+FP+DG I+ + + ++RVS++ HVFTIH +D P+ +N ++I ++ C+ + + T F+DGI +A S ++ P PN +I S++ N N + + I N N + TQ PF H I L+TPDG QV+V + E AE N+ Q N LL + K SS + + ++ S + E+ R+ITSDG V+ I + I++L+PDG TVA ++ +E K + IN++ P K+ +++ + T + T L S P + W ITL +GE F + + N+ S P ++ + D ND ++ P++ SF+ A DP+TGETL TR + L+ V + +H+DGTR T+ EK + S A ++ + E +PAVI++ + + E S GHHI +H + ++ PD + +T+ ++N Y L+ P V+ + C ++ + V+ +N + +I +N++ E++ + ++D ++N N+ SND YD+H PRLF +P EL+ + R ++ L S D +++ + RE +QN+P++ T++ S + KY Y ++ ++P+GL+ RD +T P +I+ G GKGL+I + QK K R++H N R+F +P I ++ F L Y + L + Q L + QS E + D I VL +K+ I++ I + + +++S+ + ++ + E + T +P+ ++ +L QE ++ RA+L++ P YF S G F+ Q D++ L+K L P N ++ L E T +S P L E E T N+ N L KLI + R T + YYD + +P + L + + +P YLYA+RPS+ N+ QAL+EDPVRR+ TS+IGG Q R+ RGL + P+R++ G+L G+ +F +KL+N GPE++ FKIKQ +GI V + PGPI AGLSR+L I + + LE SN + + LQ T N D
Sbjct: 876 LNKAKNIHLNEWSVEEKLEPNVLLQRLHSLNYEKPYLDVYKRLYDDSLFIISHHPFDRTIRSNHYTWCSWFHVNKIGFRPYLQLIEGHIRNWTREQEAIYEAAKLSAEILQTDDIHGSSTEQSPTKPIKKNSPKSQKGAKSNRNRSISGSESEESIVQNEIKSLLGDPNEFILPGSLKAAQRDQERAKLKKDLEERQKETKRVKSAKKPTEREISPTEKRKTSGKRNKSPHPKSPVHKNDQDDESIQP------QQLRTKRTSNE-FWPFTGYDISNLLPHITGEVTHMFPTDGGTIKTQRNEIPTGQANLRVSLMK-DGHVFTIHKIDGPPEPNIPYENPNEIKLNDQENEEGCDGLKTFSSPELFSSITACFSDGICLAVS-------RVNKIDPQPNPNYQIESNQLNKFNKNSDSDNNNDCRIRPNSVNLNDNSETTQNKPF-QHF-------IHLSTPDGAQVAVYHCVNEVSNRAIDECAEENIPSKEALINPESKCQTNDGQNNALLNSQHSILLKL-SSARTCINDSINIDDLKKRSDKEYEVTRFITSDGIVIQILMPSCTSSASCAIRILYPDGAIMERGTTVAEELRRNENPQFTLSNPQEEKQQEINIQSEPAMPSALQVINIQPRKSSSKRKSSRRKIEEKKTEDDARNETCLVSTDIATALTPSSTPRSTTNDSDDNLPWLITLLSGERFWFKLIKPLTNIGSSVEDTPTTNASDSVSHESVNLDTLNDAVKYETYPSKPMESFR------------AYDPATGETLYTRPEDNLIAVEPHPDSPFKLIVQHADGTRLTQILLASEKLNDECNSVDLNSNANKPQMFIRTECVGFPAVILNKSTGEFEISLFGKSQFSSTFHFTPKGHHILQHFNGGQLNINPDSSVYYTSQSLEIVNLNEPTVYEMRSKDMESLLECTDPHKNVYTVDNWANCNVLLTGNKRHTVDEDLNTEVNQIYSTEITNENNQQIEDSFHTVLQNKDNADNNEINE--PHNNEISNDGCCLYDEHIPRLFYFDPIKRIAIELIHHEEYKCLLRRAHNIQSPRHHINKLTMEEKLMYMNTKSQNTSTSDSDFYEGLYLREPMQNNPRVFGLTLMKSLPTNETGKY-YVQENIIPEGLSSRDFQTITHSSNSNPHSDIRK----LGERAGKGLDIIMPSLEYLGLKLPETNVTLLPAQKGKSIKRENHKFNAYYQALHANVRRFTEFPLITSEINQRFLGVLCAYTEYYLNRLNEWCSQQPTRLSEIMQS-ELRLFLDPIPTVSSVLSRNKQ-ISDPIENENITDVSSSPSQQTTVSQEISQPTN-KYSPARQEAFKLLRQEIEKAMRD---RAALRSTHTPNYFRSQEGIGFLLSQIPDVKKLSK---LVPELSNLPSQNPKQNDKSTLTEQSTLILSDSPPNIPSPLLRTIENDNELITEKITGNTNNSHLTMNKLIYNDGYKTGMKMSTRETTKKCFYYYDIDPSQIKMIDNHSTHVPLDAVALAKQKQLRVPKYLYAKRPSLPKCNNGVNGISHATNFNVKQALIEDPVRRRALFTSIIGGPPFGQFSLRRMRGLRLTPSRINVGVLPYGVTRSFSVKLVNWGPETAYFKIKQLPIQSGIRVFYTPGPIPAGLSRRLAIEVSNQRQDRLESYPSNQQIDDRGLQQTANHD 2494 HSP 2 Score: 144.821 bits (364), Expect = 1.834e-30 Identity = 122/459 (26.58%), Postives = 225/459 (49.02%), Query Frame = 2
Query: 35 RNKSPAANIASGNKWESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEAPLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGSQVPGKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYEMQ---------IENLKSFWTNINPVLNDN--KTLLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPVKTSVAQTHENTTPRESI---KEGVQASPDNTDSHM--------NFTFDKFNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTP 1345
++K+P A+I + WES + L+++ W SLS++ ++ + E I+ + I G R++FT+IT E + I F K+K K+AP FEIC + K+ ++ + L L+K +K++ + KT D+K + + K D ++ K G++V +S EPP K +KL KRG+ + +Y+ DEPD+G SRY ++ LL L + SI++ +H+ ++ + + +++ + S+ +L +M+ E L+ +W + ++ D+ L N + K + P + + + +F ++ ++YDL+D +RQWKNYL+ K+I LP+ + +++ + + + K + PDN D D ++ +YN +LQ +PIESTS+ L + A+L+QV E P
Sbjct: 9 KSKTPRASIGVAD-WESKLCDLNLDDEVWKMSLSILSPSLLTENEIISILAASIVNGQRQRFTVITYEDVLNQI--FTQCKAKSQKDAPEFFEICNVAKNAIETVDELNPQTLSKVLKFMFNSIKTLDIKQQS-----LRKSIPKTNHMEDTKKKENPKDKKSGAKV--IKSQEPPVNKAKSKLYKRGEEALEEKYLGDEPDNGISRYILLVNFEKCGLLEELCRLKIDIHSIIR----LHVNDQKQLQILITKQKAEKMWGSKGSEQDLEQMRSEEEALLLAEEQLRQYWRTTSILMRDHVYDRLGNIATLDYNVKTELLPDDLNGAENRASFGATMFNEISTILYDLIDFRRQWKNYLEHIKVIHLPICPPLLNENDSVSSNQLLKMSKPAIGLHPDNLDKTAIKSEDCGDILAEDFVDMRFYNEILQGLPIESTSVELILHAVLEQVIANENEILP 453
BLAST of Sperm associated antigen 17 vs. TrEMBL
Match: A0A433UDD6 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_000422 PE=4 SV=1) HSP 1 Score: 330.102 bits (845), Expect = 6.462e-87 Identity = 263/903 (29.13%), Postives = 434/903 (48.06%), Query Frame = 2
Query: 23 MSKKRNKSPAANI----ASGNKWESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEAPLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGSQVP---GKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYEMQIENLKSFWTNINPVLN---DNKTLLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPV----KTSVAQTHENTTPRESIKEGVQAS--PDNTDSHMNFTFDKFNID--YYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTPIWELNKSDLPKDLNEDSIDVFKKITDNIYFPXXXXXXXXXXXXIPLDDDDENRNKDQNSPIHVLDSITQRDREINTKNFGKNGALGHELQMLVASFRHFDLLKFIPTDDQISRKRAAKLQELINYLNNTGLSAIEINYAIKLFILENLTLSGVDENGVIRINNEDGYFQRNNYQVWDDPY----MILNDVAQSIID------EENKTLKXXXXXXXXXXXXXXXXXPALSFIKNENF---TEGILKXXXXXXXXXXPENIKSRRKSSVHFLETERVESPTMSTETQ---------MDIMAFINNQNDEGFQEIL-LDYLSNLRKRNFSEWCFE---------------EFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEAIYQANLLNQELQIV 2563
MSK + + ++ A G KW+ I++ E++W+ ++ ++ +D++ I +N+ + TG RK F++IT E + + D GNPK+KK KE P N EICE K ++D E +P P LAK IKW +L K D+K + +KA K + GGK+ ++ P GK++PEP + K +KLRKRG+ D D +YIDDEP+DG Y +++G D + L +I S++ + + P + D++ E+Q E L FWT++ P+L D +L + + K LM P + + + K++ + LFE +AV++YDLLD +R +K YL+ KL +PV +++ TP ++ GV ++ P + +D YYN L+ VP ES S+ L + ML+Q+ + P E P +N + + + L L++P D+ + I+V D I+ R + + ++G N A ++ + F + PT + SR RAA+LQELI+Y LS EI+ A K F+ E++ L+ VD NG I ++G Q WDDPY ++L + +D + AA + S+ S A ++ + F G +SR ++ RK S+ L + R SP T + D++A +E Q+I +D N+R + E E E ++P +LLQI+ ++ Y LP+ +IY +R + ++VL+NPF + +NH W+K LHSN+GFRNYLD V + I++W +++EA YQA++L++E++ +
Sbjct: 1 MSKAGKRGKSGDVKGGPAGGGKWDQAISSGVFQEESWNPCVTWVVGQQAEDSKHIESLNIIVQTGTRKLFSVITKELLYADVRDLGNPKNKKVKETPANSEICEACKPYVDTEEPIPLPLLAKLIKWKLLAIKATDLKR---REAEGKALKAKAVSEKKKGNKDAKDGGKERAKSPKKGGKKTPEPQTAKDGSKLRKRGEEDSDNKYIDDEPEDGCQHYILVTGFHDPRIFQFLEEIKVTVDSVIGIRSQDYTPLKRPPPPEAEPPAEPTEEDRK-----FKELQDE-LCLFWTDLLPLLQRLPDASSLHDIARLDYEVKALMVPSDPMDTEQKVQYSTSLFEDVAVMIYDLLDARRLYKTYLENLKLTRIPVYGLEESTAITPVPPPTPGAAVAPGVTSAVEPGGAAAAPESRIPPPPVDMRYYNDLMNTVPQESVSVPLILHCMLEQITANEEGREPPSEQAPPIRPDGVNFHLASYLTGVAFKLGLTESEHQMLSEVLELPSPPPDQPGPPEL---INVQDDISNRTQHLQ-PHYGFNPAAVEKVMLQFVPFAKLWHHQQRPTSAE-SRLRAARLQELIHYCVTEELSKSEIDRAFKQFVFESMDLATVDSNGFILDKGKEGLHQ--TAVPWDDPYPFFKVMLPQNKRPSLDIRCSSLSSEDSRSATAAQHRRASLQQERSRDAPAYTNGKMFGGTGRGACTPEIDTSRESRRGSLDESRKKSI--LVSARTSSPRDGTPKESRSNSVHWDADVLA------EEPIQDIQDVDMPPNIRTKTVEEGVLEMVEAQQRNLDQWCFAEHFKPDILLQILREASYMLPFHEIYHHKRDETHMLVLYNPFGAEL-HNHVDWHKELHSNMGFRNYLDCVAESISEWLKEKEAEYQAHVLSKEVESI 878 HSP 2 Score: 162.155 bits (409), Expect = 1.009e-35 Identity = 117/437 (26.77%), Postives = 209/437 (47.83%), Query Frame = 2
Query: 4187 ATDPSTGETLVTRSDGTLLLVSQDAGIFLCEHSDGTRFTKYFNVEKSSSSTYEPGEISRAKISELLL----IESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDGEIIFTASVNSQMSYLQPDGPFSYVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQK----YDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILHSDSKIKP--------WKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIYS---NPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVNQSDEEKKIADEILEK-FKVL 5437
ATDPST +T+ TR D ++ VS G + EH+DGTR T Y+ +++ S E E+ A +S L +E + V + + + T GS ++ G++I HS IE +G +++ + + +L P+ YV +++I E +D +G F V G+++V + +D ++++DS ++ K Y QHAPR F I+ DGSG+ELLRY+D+ + L+ ++ + ++ + + P + T+L KP W Y +++++P G+ CRD+KT PPKE GP FG +VG+GL + + P+ ++ P+K ++ RQ Y + +RS + L Y + ++ + + E + D+ + + ++IL + KVL
Sbjct: 1459 ATDPSTSQTMATRDD-HVITVSYPDGTTVVEHADGTRITVYYR--ETAMSVEENDEVCSAIVSTQTLKFVKVECPAFATVEFNTSTSENLTVFGSGTSINVFPDGYYILHHSKGGRIEVDTEGTMVYFPRRSRFLEHLMPERELQYVLRHNADIILETVDPDGNVFNVRSTGDYNVIPVQADD--------------------------ALSDDSEEHTSKKIAHYGQHAPRFFIIHGDGSGTELLRYQDVAEYLMLAEQNPATAVLKDVLPDHPGVKGITVL------KPYLLGLSEKWFKNYDQESIVPSGIRCRDLKTLPPKEFVKPGPKFGTNVGQGLNVGAANTGPVRVPILKCPRK----------LELRQVVQYKPMADSLRSVLRKGLRSYAEAVVGRMR----AEALTNIDDIRTVDEQILAQDLKVL 1846 HSP 3 Score: 137.117 bits (344), Expect = 3.756e-28 Identity = 70/164 (42.68%), Postives = 99/164 (60.37%), Query Frame = 2
Query: 6008 INLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSLIGGSETSQILQ-RKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIANNLEKNSSNVSLLEHFLQITTNTDQLIIPIVGRVMTAENY 6496
+ LP + RP PN+ +EDPVRRKV+T+ G +E ++ Q RGL P V+ G+LKEG Y + + L NTG ++ FKI+QP +TG+ V + PGP+AAG+ +L + +YAIAN +E S V L H L+I T TD L +PIV ++TA Y
Sbjct: 2129 VPLPGSIKGGRPGAMPNVKYHTIEDPVRRKVFTSLTAGAAERGRLPQLAGMRGLICQPEGVNFGVLKEGCTYRYAVMLRNTGVDTCRFKIRQPPPATGLRVIYKPGPVAAGMCVELALELYAIANGVE-GESGVGSLGHVLEIITETDLLELPIVATILTAHEY 2291 HSP 4 Score: 66.2402 bits (160), Expect = 1.433e-6 Identity = 29/66 (43.94%), Postives = 40/66 (60.61%), Query Frame = 2
Query: 2990 EEYWPFISYHLDNDLAHFMGMVAHLFPSDGSIIRVFKMQKLLDTTSVRVSVITTQSHVFTIHILDP 3187
E YWPF Y N L H G+ LFPSDG I+ + + + TT++R SV+ HVF+IH+L+P
Sbjct: 1018 EPYWPFYGYDSGNQLIHVSGITTTLFPSDGGTIKTERTEFVQGTTAIR-SVLQKDGHVFSIHVLNP 1082
BLAST of Sperm associated antigen 17 vs. TrEMBL
Match: A0A094ZYX3 (Sperm-associated antigen 17 OS=Schistosoma haematobium OX=6185 GN=MS3_06764 PE=4 SV=1) HSP 1 Score: 319.316 bits (817), Expect = 1.635e-83 Identity = 424/1656 (25.60%), Postives = 699/1656 (42.21%), Query Frame = 2
Query: 2210 QEILLDYLSNLRKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSN-VGFRNYLDFVHDKITDWNRDQEAIYQANLLNQE-LQIVETEIKETEVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTQEL---------SLIVPGSLKAQKLXXXXXXXXXXXXXXXXXXXRQLSAQKKTEKDLAKNVEKTKDSKRPXXXXXXXXXXXXXXFEDSGENFDKENYQEIYTDRIDGEEYWPFISYHLDNDLAHFMGMVAHLFPSDGSIIRVFKMQKLLDTTSVRVSVITTQSHVFTIHILDPTYSPDSQIDNFDQILEQFSHSKNICNYGASTCTFNDGITIAASNNGYSGSQLKNKSPIGAPNDEISSHR-----SNDLTVNGNKEEIFQNDTNETNEKIITQKNPFVNHMDYIDSPNIILTTPDGVQVSVGY-----------ESAE------------------NENQRNLLL-----FMCKFESSLKPSVPPELS-------SIQAPEIGRYITSDGRVVTIF-------KNELIKVLFPDG-------TVAXXXXXXXXXXXXDDEVEEFKIENINVKESP---------------------------NSKRKTKQSKSISVEPQTPLNTVLVEDSKPSCNYSRK-----WTITLSNGE-----VFETVANLNSIFNPKPLIETIET------KLDDEATTDENNDILEANQPTEKERSFKSLQTKERLLTSYATDPSTGETLVTRSDGTLLLVSQDAGI---FLCEHSDGTRFTKYFNVEKSSSSTYEPGEI-SRAKISELLL-IESKQYPAVIIDLTNEKIETRCGSMGALLSN----SCGHHIFKHSDNTEIEYRPDGEIIFTA------SVNSQMSY----------LQPDGPFSYVFNPTSNVICEIIDSEGTE-FIVN------INGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTND-SNDNVQKYDQHAPRLFTINP-DGSGSELLRY----------------RDIVDTLV--------------NSDIDENYF---HCREEIQNSPKIIAHTILHSDSKIKPWKYEYSRKTVLPKGLTCRDMKTF-------PPKEIKTDGPPFGMSVGKGLEIY------------SNPIMAKNVQKPKKSFRQDHWLNP------MQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQ-----RQLYKDLEIVNQSDEEKKIADEILEKFKVLKFSKESIANAIGS--LVNINSTQGEMSSKAFETNAKTALPKNPSHRKLDVILEQXXXXXXXXXXXRASLKNHIVPKYFTSIYGKDFISKQAMDLQSLNKSMKLFPISREGTDVNDLKDKNEFLNETET-SEVSLLSPKFQVDYLEQSPEN-------STKNSKNLLKPKLIIQKRATNY-----LYYDSN--LFIPNEPKRLPELSNINLPNYLYAERPSIEP--------------NIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIY-AIANNLEKNSSNVSLLEHFLQITTNTD 6448
QE L+ + + +EW EE EP+VLLQ +H Y+ PY+D+Y + D ++ H+PF + NH++W W H N +GFR YL + I +W R+QEAIY+A L+ E LQ + TE + K +KS+ + GS +EE+ Q I+PGSLKA + +QE+ K EE +KE KR SA+K TE++++ ++ KR S K ++S + Q++ T R E +WPF Y + N L H G V H+FP+DG I+ + + ++RVS++ HVFTIH +D P+ +N ++I ND +N+ P PN +I S++ N + N N I N N + TQ PF + + + +TPDG QV+V + E AE N+ Q N LL + K SS + + ++ S + E+ R+ITSDG V+ I + I++L+PDG TVA ++ +E K + IN++ P K+ +++ + T + T L S P + W ITL +GE +F+ + N+ S P ++ LD ++ N+ + P++ SF+ A DP+TGETL TR + L+ V + +H+DGTR T+ + + P ++ S A ++ + E +PAVI++ + + E S GHHI +H + ++ PD + +T+ ++N Y L+ P V+ + C ++ + G + VN +N + ++ +N++ E++ + ++D ++N N+ SND YD+H PRLF +P EL+ + R ++ L S D +++ + RE +QN+PK+ T++ S S + KY Y ++ ++P+GL+ RD +T P +I+ G GKGL+I + QK K R+ H N R+F +P I ++ F L Y + L + Q L + QS E + D I VL +K+ I+ I + + +++S+ + ++ + E + T +P+ ++ +L QE ++ RA+L++ P YF S G F+ Q D++ L+K L P ++++L +N N+ T +E S L L SP N + +N L+ K+ ++ +Y D N +P + L + + +P YLYA+RPS+ N+ QAL+EDPVRR+ TS+IGG Q R+ RGL + P+R++ G+L G+ +F +KL+N GPE++ FKIKQ +GI V + PGPI AGLSR+L I + + LE SN + + L+ T N D
Sbjct: 832 QENFGTLLNKAKNIHLNEWSVEEKLEPNVLLQRLHSLNYEKPYLDVYKRLYDDSLFIISHHPFDRTIRSNHYTWCSWFHVNKIGFRPYLQLIEGHIRNWTREQEAIYEAAKLSAEILQTDDINGSSTEQSPTKPIKKNSPRSQKGAKSKRNRSISGSESEESIVQNEIKSLLGDPNEFILPGSLKAAQRDQERAKLKKDLEERQKETKRVKSAKKSTEREISPTQKRKTSGKRNKSPHPKSPVQKNDQDDESIQQ------QQLRTKRTSNE-FWPFTGYDISNLLPHITGEVTHMFPTDGGTIKTQRNEIPTGQANLRVSLMK-DGHVFTIHKIDGPPEPNIPYENPNEI------------------KLNDQ---------------ENEQP--NPNYQIESNQLNKSNKNSDSDNNNDCRIRPNSVNLNDNSETTQNKPFQHFIHF--------STPDGAQVAVYHCVNEVSNRAIDECAEENIPSKEALINPESKCQTNDGQNNALLNSQHSILLKL-SSARTCINDSINIDDLKKRSDKEYEVTRFITSDGIVIQILMPSCTSSASCAIRILYPDGAIMERGTTVAEELRRNENPQFTLSNPQEEKQQEINIQSEPAMPSALQVINIQPRKSSSKRKSSRRKIEEKKTENDARNETCLVSTDIATALTPSSTPRSTTNDSDDNLPWLITLLSGERFWFKLFKPLTNIGSSVEDTPTTNASDSVSHESVNLDTLSSKRLNDAVKYETYPSKPMESFR------------AYDPATGETLYTRPEDNLIAVEPHPDSPFKLIVQHADGTRLTQILLASEKLNDECNPVDLNSNANKPQMFIRTECVGFPAVILNKSTGEFEVSLFGKSQFSSTFHFTPKGHHILQHFNGGQLNINPDSSVYYTSQSLEIVNLNEPTVYEMRSKDMESLLECTDPHKNVYTVDNWANCNVLLTVGNKKHTVNEDLNTEVNQIYSTELTNENNQQIEDSFHTVLQNKDNADNNEINE--PHNNEISNDGCCLYDEHIPRLFYFDPIKRIAIELIHHEEYKCLLRRAHNIQSPRHHINKLTMEEKLMYMNTKSQNTSTSDSDFYEGLYLREPMQNNPKVFGLTLMKSLSTNETGKY-YVQENIIPEGLSSRDFQTITHSSNSNPHSDIRK----LGERAGKGLDIIMPSLEYLGLKLPETNVTLLPAQKGKSIKREIHKFNAYYQALHANVRRFTEFPLITSEINQRFLGVLCAYTEYYLNRLNEWCSQQPTRLSEIMQS-ELRLFLDPIPTVSSVLSRNKQ-ISYPIENENITDVSSSPSQQTTVSQEISQPTN-KYSPARQEAFKLLRQEIEKAMRD---RAALRSTHTPNYFRSQEGIGFLLSQIPDVKKLSK---LVP------ELSNLPSRNPKQNDKSTLTEQSTL-------ILSDSPPNIPSPLLRTIENDNELITEKITGNTNNSHLTMNKLIYNDDNHSTHVPLDVVALAKQKQLRVPKYLYAKRPSLPKCNNGVNGISHATNFNVKQALIEDPVRRRALFTSIIGGPPFGQFSLRRMRGLRLTPSRINVGVLPYGVTRSFSVKLVNWGPETAYFKIKQLPIQSGIRVFYTPGPIPAGLSRRLTIEVSNQRQDRLESYPSNQQIDDRGLKQTANHD 2394 HSP 2 Score: 129.798 bits (325), Expect = 6.897e-26 Identity = 110/417 (26.38%), Postives = 199/417 (47.72%), Query Frame = 2
Query: 161 DTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEAPLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGSQVPGKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYEMQ---------IENLKSFWTNINPVLNDN--KTLLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPVKTSVAQTHENTTPRESIKE---GVQASPDNTDSHM--------NFTFDKFNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTP 1345
+ E I+ + I G R++FT+IT E + I F K+K K+AP FEIC + K+ ++ + L L+K +K+++ + KT D+K + + K D ++ K G++V S EPP K+ +KL KRG+ + +Y+ DEPD+G SRY ++ LL L + SI++ +H+ ++ + + +++ + S+ +L +M+ E L+ +W + ++ D+ L N + K + P + + + +F ++ ++YDL+D +RQWKNYL+ K+I LP+ ++ + + +K + PDN D D ++ +YN +LQ +PIESTS+ L + A+L+QV E P
Sbjct: 13 ENEIISILAASIVNGQRQRFTVITYEDVLNQI--FTQCKAKSQKDAPEFFEICNVAKNAIETVDELNPQTLSKVLKFMLNSIKTLDIKQQS-----LRKSIPKTNHMEDTKKKENPKDKKSGAKV--IISQEPPVNKEKSKLYKRGEEALEEKYLGDEPDNGISRYILLVNFEKCGLLEELCRLKIDIHSIIR----LHVNDQKQLQILITKQKAEKMWGSKGSEQDLEQMRSEEEALLLAEEQLRQYWRTTSILMRDHVYDRLGNIATLDYNVKTELLPDDLNGAENRASFGATMFNEISTILYDLIDFRRQWKNYLEHIKVIHLPICPPSLNENDPVSSNQLLKMSKLAIGLHPDNLDKPAIKSEDCGDILAEDFVDMRFYNEILQGLPIESTSVELILHAVLEQVIANENEILP 416
BLAST of Sperm associated antigen 17 vs. Ensembl Cavefish
Match: si:dkey-208b23.5 (si:dkey-208b23.5 [Source:ZFIN;Acc:ZDB-GENE-081104-362]) HSP 1 Score: 140.198 bits (352), Expect = 4.269e-33 Identity = 110/420 (26.19%), Postives = 197/420 (46.90%), Query Frame = 2
Query: 77 WESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEAPLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSG--GKKGSQ-VPGKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYEMQIENLKSFWTNINPVLNDNKT---LLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPVKTSVAQTHENTTPRESIKEGVQASPDNTDSHMNFTFDKFNIDYYNFLLQDVPIESTSIYLQMSAMLDQV 1318
W+S + A E++W S+ +++ +D +N + + LRK FTL++ E + IN+ GNPK++K K+ P+ +E+ E+ +S LD E L +AK +K+ +L K D++ +K G+ + A A DK+ + + G KKG + P K + KL++RG+ + +YI DEPDDG Y +++G L++ L +G + ++++++ D + +DKE+ + ++L FWT ++ VLN L + + + + P + E+ + +FE +A L+YD LD +R + YL+ KLI +P + A E G+ A D + Y+ LL +P + S+ L + ML+QV
Sbjct: 23 WDSALCTAGFEEESWKASVCLVVAGSVEDEVCVNALKRAVQQPLRKLFTLLSWENTLEKINELGNPKTRKIKDLPMYYEVMELARSELDTGEELSVDLVAKLVKFQLLNIKNSDLQRRA-VEEKAKGRAAPASAAKDKTAKVSAKGEKAKKGLEAAPAK----------ETKLKRRGEEEETTKYI-DEPDDGPQHYILMTGFQQPQLISLLDSLGILVSTVIRLISD--------------PPQDPAAADKEHLKS------AQDLDVFWTKLDTVLNVGGVGSRLHDVAKLDYRVERSLLPTDPEDTQ---SLGVTVFEGVACLLYDSLDWRRHHQLYLNSMKLIHVP-RVGRADRPERV--------GLSAEVD--------------MRLYSDLLNQMPAKIVSVPLILHCMLEQV 384 HSP 2 Score: 53.5286 bits (127), Expect = 2.709e-6 Identity = 25/103 (24.27%), Postives = 47/103 (45.63%), Query Frame = 2
Query: 2222 LDYLSNLRKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEAIYQ 2530
++ L + R W E ++PH+ Q++ +D + D + HNP P + + W LH+++ F NYL+ V +++ DW R ++ Q
Sbjct: 573 MEELQKMLTRRLRHWTITEHHDPHIFPQVLQAVSETYRCVDTFTSSSDDTIFTISHNPMGPQ-RSSKECWEVCLHTDIRF-NYLEHVAERVRDWTRAEDQKQQ 673
BLAST of Sperm associated antigen 17 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009916.1 (pep scaffold:Pmarinus_7.0:GL476547:717219:757547:-1 gene:ENSPMAG00000008955.1 transcript:ENSPMAT00000009916.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 195.667 bits (496), Expect = 4.579e-50 Identity = 160/637 (25.12%), Postives = 284/637 (44.58%), Query Frame = 2
Query: 110 EDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEAPLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGSQV---PGKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYE----MQIENLKSFWTNINPVL---NDNKTLLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALP-VKTSVAQTHENTTPRESIKEGVQA------SPDNTDSHMNFTFDK---FNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTPIWELNKSDLPKDLNEDSIDVFKKITDNIYFPXXXXXXXXXXXXIPLDDDDENRNKDQNSPI------HVLDSITQRDREINTKNFGKNGALGHELQMLVASFRHFDLLKFIPTD-----------DQISRKRAAKLQELINYLNNTG--LSAIEINYAIKLFILENLTLSGVDENGVIRINNEDGYFQRNNYQVWDDPYMILNDV 1903
+D W S++M++ +D I + I LR+ FT IT + + IN+ GNPK K+ K+ P+ +EI E+ K+ +D +A+ P LAK IK+++L K +D + NK + K + + E KS K+G GK+ PEPP +K+D KL +RG+ Y DDEPDDG Y + G + LL L ++G ++++ V+ + + ++ N D S ++ E ++E L++FW ++PVL +L + + + P + +++ +K LFE +A LMYDLLD ++ +++YL+ +L+ +P + S+ +T ++ G+ A P T ++ ++ +Y LL +P E S+ L + +L+QV T + P +L L+ D + + FP DD+E+ + + H+ I + EI ++ A G ++ + D+L+ +P D +R RA QEL+ Y + + + +A++ F+ ENL LS VDE G + + ++ N WD+P +V
Sbjct: 11 KDGWQASVAMVVCGRVEDEAHIPAMLQAIRVPLRRLFTAITWDDVMTQINELGNPKGKRPKDLPVYYEILEVAKTAVDAGQAISLPVLAKLIKFMLLGVKQKDQQWREAKNKAAEEKAKEMAEVDCGKEPVKSPVKKRGKSAKGNKGKKLPEPPQIKKDTKLMRRGEQGHLRCYSDDEPDDGPQHYVFLMGFLQPQLLPMLKELGVTVSAVICVSSQTYT---EDKPAQAHATKMLNSCDLSLSGGDIVEELLAGRVEELQAFWRYLSPVLEGVGHGYSLDTVARLQYTVHNSLLPEDPADQNTMVKFGAALFEDMACLMYDLLDWRQHYRHYLNNMELLQIPTIGQSLVRTDNEAMQASEVRNGISANGKRKGQPSETTDLPPLVREEPSAVDMRFYRDLLDPIPPECVSVPLMIHCILEQVVATEEGRPPPSQLRDPPRADGLHPDLANSLSAAANR--FPLS-------------PDDNESLAALFGTSLCSLGQEHIKPKILSFNDEIMSRTHHLQAADGLDVAQI-----EKDMLRRLPVCGMLLDPGLHKVDNATRIRA---QELLQYCSGCAGQVEQASMQHAVRQFVFENLELSSVDEAGELTVVHQ----ADNVALAWDNPLAFAKNV 617 HSP 2 Score: 192.971 bits (489), Expect = 3.214e-49 Identity = 207/820 (25.24%), Postives = 345/820 (42.07%), Query Frame = 2
Query: 4187 ATDPSTGETLVTRSDGTLLLVSQDAGIFLCEHSDGTRFTKYF-NVEKSSSSTYEPGE--ISRAKISELLLIESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHH----------IFKHSDNTEIEYRPDGEIIFTASVNSQMSYLQPD-GPFSYVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQKYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILHSDSK--IKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGL------EIYSNPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVNQSDEEKKIADEILEKFKVLKFSKESIANAIGSLVNINSTQGEMSSKAFETNAKTALPKNP-------------------SHRKLDVILEQXXXXXXXXXXXR---ASLKNHIVPKYFTSIYGKDFISKQAMDLQSLNKSMKLFP--------ISREGTDVNDLKDKNEFLNETETSEVSLLSPKFQVDYLEQSPENSTKNSKNLLKPKLIIQKRATNYLYYDSNLFIPNEPKRLPELSNINLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSL--IGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIANNLEKNSSNVSLLEHFLQITTNTDQLIIPIVGRVMT 6484
ATDP TG ++TR D + + D G + +H DGTR T Y ++ +S E GE + + + + +ES P + T GS L + G + +F SD E Y P + + +++S P P +Y + + CE +D G F V NGE + E+ E Q +YD HAPRLF I+ DGSG+EL+R D L ++ R+ + + P + T+L K + W + + P+ L R +TFP +E K GP FG +G+GL E+ P ++ V+K R RQ YP + ++R L +Y +++LQ+ + ++++I ++ ++ADE ++LK S+ N + + E++ +E + T P P S R + E+ +++ + + +LK+ I+ YF+S G+ F+ QAMDL++L+K + P + E D L D E S V ++ P F + + ST + + L + AT + L + +R + LP + A RP N A V + + ++S+ +G S S++LQ G + P RV+ G L++G + +I+L N G E FK+K P SSTGI V + PGP+AAG IHI I LE + N+ + + +QI T + L++P+ +++
Sbjct: 1388 ATDPQTGAVMITREDKVVTVTDAD-GRIVADHVDGTRITTYHRHILDHNSDEQETGEGMVRTVRRVKCVRVESVGLPTLQCRPDEGSCITLFGSGTVLHAKPDGTYQLCPSGGEGSLFIASDG-EARYSPGR--VSSPALDSLPGTRDPALTPGTYFLRHVAPLACEAVDVYGNHFQVLCNGEASTSLAGAAGAGAEDERGDGEEAQ--------------------KPARYDTHAPRLFAIHADGSGTELIRREDAERFLSWAEGHPITTVIRDTLPDHPAVQGVTVLSPVEKDVRQRWLLQKQESDITPQSLRSRQWRTFPAREEKKAGPRFGTCIGRGLTIDRPCELNVRPAVSPCVEKRPDVLRA---------RQLLCYPAVTTEMRCKLQLCLKEYLEQLLQREKQREEMKI-----QDPRLADEKTHISQLLKLVL-SMPEDSPRYYNEDERRAELAD-LYEQSFTTQPPSFPPDAQAEQEYFKKSLWREEASQRTQALWAEKIAKERQEVEQAKEDIQALKHKIITPYFSSELGRTFLLSQAMDLEALSKEVPHTPRKPSPALDCTSEDGDSCHL-DYRPMCWAAEGSAVPMM-PAFTTSVFIAN-KMSTMTVPAVAERHL---RDATRHHAKSLALDVTGSARR----ERVRLPAAVLASRPRSVHNEKVARVSQSHPKIMESSSITNVGASARSEVLQ----GFTLLPTRVNFGCLRQGSSNVRRIRLQNVGTEQHRFKVKAPPSSTGIRVIYTPGPVAAG------IHIELI---LELKAENLGPVSYEVQIQTEAETLLVPVTANILS 2144 HSP 3 Score: 63.5438 bits (153), Expect = 5.944e-10 Identity = 34/91 (37.36%), Postives = 54/91 (59.34%), Query Frame = 2
Query: 2249 RNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVL-HNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQE 2518
R +W + E YEP VL+Q++ ++ + +D Y + +DG L++ HNP D + W+ LH+NV FR+YL+ V + +TDW R QE
Sbjct: 751 RCLDDWNYTERYEPDVLIQVLVEASERYICMDTY-RLNLDGSLLLFFHNPMS-DELQSLEYWDIALHTNVSFRDYLEHVAELLTDWLRGQE 839
BLAST of Sperm associated antigen 17 vs. Ensembl Nematostella
Match: EDO30301 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T172]) HSP 1 Score: 117.472 bits (293), Expect = 5.325e-26 Identity = 190/918 (20.70%), Postives = 347/918 (37.80%), Query Frame = 2
Query: 2990 EEYWPFISYHLDNDLAHFMGMVAHLFPSDGSIIRVFKMQKLLDTTSVRVSVITTQSHVFTIHILDP----------------TYSPD------SQIDNFDQILEQFSHSKN---------------ICNYGASTCTFNDGITIAASNNGYSGSQLKNKSPIGAPNDEISSHRSNDLTVN------------GNKEEIFQNDTN---------------------ETNEKII--TQKNPFVNHMDYIDSPNIILTTPDGVQV---------SVGYESAENENQRNLLLFMCKF---ESSLKPSVPPELSSIQAPEIGRYITSDGRVVTIFKNELIKVLFPDGTVAXXXXXXXXXXXXDDEVEEFKIENINVKESPNSKRKTKQSKSISVEPQTPLNTVLVEDSKPSCNYSRKWTITLSNGEVFETVANLNSIFNPKPLIETIETKLDDEATTDENNDILEANQPTEKERSFKSLQTKERLLTSYATDPSTGETLVTRSDGTLLLVSQDAGIFLCEHSDGTRFTKYFNVEKSSSSTYEPGEISRAKIS----ELLLIESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDG-----------EIIFT-ASVNSQMSYLQPDGPFS-----YVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQKYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFH--CREEIQNSPKIIAHTILHSDSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIYSNPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVNQSDEEKKIADEILE 5422
EE++ FI Y + ++L H G ++ LFP DG IRV K + +VRVSV ++F +H L+P T P+ + +D ++ +E+ N ++ + F+DG+T+ S G SG +L K +G D ++ D + G+ + + D + E N++ QK PF N + +T PDG+ + S+G QR + K E+ LKP++ E R + +DG ++ + + ++VLFPDG +A K ++ +K + S EPQ P + + S+ S+ P P+I E + AT ++ I+ QP + ++ + +L S T P +G+ L TR D ++ V + G F+ EH+DG+R T ++ ++ + + S+A+ S + + +E + ++ + +T G+ + + + G + D + + G +I+F A S+ S D P YV CE+ D++G+ F V NG V+ + + P +Q + +++D K L S + AP + +LV SD D N + C + ++ H+ + K W + T++P+ L RD K+ P E KT GP FG +VG G+ + + P K +P + + ++FRQ Y + R + + +Y++ + ++ + + + +E +K+A E LE
Sbjct: 484 EEHFDFIGYDVGDNLIHASGSLSTLFPCDGGQIRVDKTSYVQGPQNVRVSVYK-DGNIFVLHFLEPTEEILTIEEIEGAQEITEKPEETATDVASVDIKEEGIEKSETEGNEKKTKEEPKPKGPKPFSDFSSFVAQFSDGMTLTHSGYGPSG-RLHGK--VGKEPDSLAGPVPTDPSATTHGTPSPQPAKVGSPKARKKADEDAKRLEELQQQQQEEERARLVEEANQRAAEERQKKPFQN---------LYITCPDGLHLEYFNDGTYDSIGDRGGVGVRQRYPVRGGGKQHCDEARLKPAME---------EQSRTVLTDGTIIKLMTDGSVQVLFPDGALA-------------------KCPSLPIKTQEPVSKPNSASGPRPQEPQQPKKLIRPQGSQ-------------------------KSVEEPMPVISVPEKIIKWTATGNDGRRIM--TQPDGE------VEVVDPVLMSSVTCPESGQVLRTRED-NVVSVERPDGTFIVEHADGSRITTFYRHQQQQTQAPDNETGSQAQYSTVSGKFVQVECPGFSTLVFECKTGSCQTLFGNGVEMKTAADGETVIDRLDGSRLHLDAKGVMSFVPRELADKIVFPPAKTTSEPSDTPSDTPSDTPAGVYVMRTNRQNCCELQDTKGSRFCVISNGNVTVERDCA-ENPESRDAQTVTSEED--KPASLESTVVT--------------AP--------------------LTSLVPSDCDGNIYVNVCDDGFED------HSYVDECGISKIWLRQKDEDTIIPRALRERDFKSLPSHEDKTPGPKFGTNVGAGVSVGTVP---KQEAQPLITCPRA-----LEFRQLIQYRPLTLHDRQLIVKAIENYSEYVEKREKRLDSILPQDPREESEKLAAEQLE 1275 HSP 2 Score: 107.071 bits (266), Expect = 7.533e-23 Identity = 52/123 (42.28%), Postives = 74/123 (60.16%), Query Frame = 2
Query: 6008 INLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIA 6376
+ LP + + RP PN +EDPVRR+V+T S+ GG++ S RG + P +V G LKEG Y + L N G +S FK+KQP STG+ V + PG +AAG+S KL++ I+A+A
Sbjct: 1582 VRLPTSILSSRPGALPNTQFMEIEDPVRRRVHTVSVAGGNQLSDGRMENLRGFELLPPKVSFGTLKEGCTYINTVLLKNVGIDSCRFKVKQPPPSTGLRVLYNPGLVAAGMSAKLDVEIFAVA 1704 HSP 3 Score: 89.3521 bits (220), Expect = 1.677e-17 Identity = 40/90 (44.44%), Postives = 56/90 (62.22%), Query Frame = 2
Query: 2249 RNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQE 2518
RN +WCF E + + LLQ++ ++ + LPY+D Y +R L+ LHNP D + + SWN LHSNVGFRNYLD V I W +++E
Sbjct: 254 RNLDDWCFMEHLDHNTLLQVLKEAQFTLPYLDTYYHKRDHTLLLALHNPVGQDGR-SSSSWNSKLHSNVGFRNYLDHVVSSIDSWVQEKE 342
BLAST of Sperm associated antigen 17 vs. Ensembl Nematostella
Match: EDO30653 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T054]) HSP 1 Score: 104.76 bits (260), Expect = 2.849e-23 Identity = 81/261 (31.03%), Postives = 132/261 (50.57%), Query Frame = 2
Query: 5 NRIISKMSK-KRNKSPAANIASGNKWESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEA-------PLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGS-----QVPGKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSS--RYFVISGLVDHLLLASLADIGCITTSIVKV 742
++I KMSK +R KS + N S +WE + A NED+W S+ N D ++ ++ I+TGLRK F++IT + + + ++G P++ GK P E+CE K+ +D+ E +P +AK +K+ +L K +D + K S K + SG + S + S PPS K+++KL+KRGD + + IDDEPD+ S Y +I G++ +L L+DIG +I++V
Sbjct: 69 SQIQRKMSKSRRGKSASTNEIS--RWEQELLQAPFNEDSWKSSVVFTAPNSVDDNIFLDILSDVIATGLRKLFSVITYDGLIKDAREYGKPQTGGGKGKGAASSKPPPFHEVCEPVKALVDQGEPIPPALMAKLLKFKLLHIKQKDFDRREEERKAAKAAESGKKDEKGGKKSHSPSGKRSKSPGKVKKGKKGESDMPPSPKKESKLKKRGDVEDTFKTIDDEPDENGSPQHYVIIHGVLMAPVLQHLSDIGISVDAIIRV 327
BLAST of Sperm associated antigen 17 vs. Ensembl Nematostella
Match: EDO27462 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T968]) HSP 1 Score: 83.9593 bits (206), Expect = 3.259e-17 Identity = 40/90 (44.44%), Postives = 56/90 (62.22%), Query Frame = 2
Query: 2249 RNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQE 2518
RN +WCF E + + LLQ++ ++ + LPY+D Y +R L+ LHNP D + + SWN LHSNVGFRNYLD V I W +++E
Sbjct: 166 RNLDDWCFMEHLDHNTLLQVLKEAQFTLPYLDTYYHKRDHTLLLALHNPVGQDGRSS-SSWNSKLHSNVGFRNYLDHVVSSIDSWVQEKE 254
BLAST of Sperm associated antigen 17 vs. Ensembl Nematostella
Match: EDO27342 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T9I3]) HSP 1 Score: 58.151 bits (139), Expect = 1.085e-8 Identity = 49/175 (28.00%), Postives = 83/175 (47.43%), Query Frame = 2
Query: 113 DNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEA-------PLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGS-----QVPGKRSPEPPSVKQDNKLRKRGD 601
D+W S+ N D ++ ++ I+TGLRK F++IT + + + ++G P++ GK P E+CE K+ +D+ E +P +AK +K+ +L K +D + K S K + SG + S + S PPS K+++KL+KRGD
Sbjct: 1 DSWKSSVVFTAPNSVDDNIFLDILSDVIATGLRKLFSVITYDGLIKDAREYGKPQTGGGKGKGAASSKPPPFHEVCEPVKALVDQGEPIPPALMAKLLKFKLLHIKQKDFDRREEERKAAKAAESGKKDEKGGKKSHSPSGKRSKSPGKVKKGKKGESDMPPSPKKESKLKKRGD 175
BLAST of Sperm associated antigen 17 vs. Ensembl Medaka
Match: ENSORLT00000034404.1 (sperm associated antigen 17 [Source:NCBI gene;Acc:101170055]) HSP 1 Score: 134.035 bits (336), Expect = 1.234e-30 Identity = 111/434 (25.58%), Postives = 214/434 (49.31%), Query Frame = 2
Query: 56 NIASGNK---WESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEAPLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGSQVPGKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYE----MQIENLKSFWTNINPVLNDNKTLLNTPIITLVA-KHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPVKTSVAQTHENTTPRESIKEGVQASPDNTDSHMNFTFDKFNID--YYNFLLQDVPIESTSIYLQMSAMLDQVEIT 1327
N+A+ +K E+++ A+L+ED+W +S+++E P D + I + + RK FTL+T I I++ GN K KK + + E+ + + LD E +P +AK +K +L K+ D ++++ + +K D +++ ++S K ++ P K P +++ KL +RG+ +P YIDDEPD G Y ++ G LL+A+L IG +++K+ D E +++ + S+ + + + E +Q L FW+ + VL+ L ++ + +V +++ ++ + +++ +FE +A L+YD L +R+ ++Y +K +L+ +P + P + SP NT + + +D YYN LL +P E+ S+ L + ML+QV I+
Sbjct: 12 NVATADKDTGEEASLLGAQLDEDSWRSCVSLVVEKSPADEDLIQALASAVQRPQRKLFTLLTRNSIVTKISELGNTKGKKPEYVTVFIELLQSARVWLDAGEDIPVDLMAKILKLQLLQVKSSDQHRR--EAEQIEAEKAKGDPPPDIADKTRAS--DKRAKTPSK--PVTSVMEKKTKLLRRGEVEPPT-YIDDEPDWGPQHYVLLVGFYQPLLIAALDGIGVHVANVIKLCCD-----RTQIRRKEQEQQSCDDSEPSQAASAISEAELAVQATKLDLFWSGMRAVLDSG--LPHSRLHDVVPLSYIVQDFHTQDPNDRMELGSRIFEGVANLIYDCLQWRREHQHYRNKLQLLHVPSISGSGYKPAEDAPTSPFQ-----SPINTKKPVCEESQRNQVDMCYYNSLLDLIPPEACSVPLMLHCMLEQVVIS 426 HSP 2 Score: 68.9366 bits (167), Expect = 8.161e-11 Identity = 69/348 (19.83%), Postives = 142/348 (40.80%), Query Frame = 2
Query: 4376 ELLLIESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDGEIIFTASVN---SQMSYLQPDGPFSYVFNPTSNVICEIIDSEGTEFIVNINGEFDV-KINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQKYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILH--SDSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIYSNPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVNQSDEEKK 5401
+++++E + V++ + + N+ G + S ++ + DG+ I+++ +Q +++ + +Y + T V C I D +G F V +G+ V K++ + +SQ +++ G + K+ H PRLF ++ DGSG+E L + L + D +E + N P TIL S W V P L R FP E ++ GP FG ++G L + + +P +S + ++ R+ + K +R + +L + + +++ QL ++ ++ + EE++
Sbjct: 1295 QVVMVEKEGQATVVMHPQRHTAQAFLADGTVVTGNNQGEYEVFPSSAGFLQIQRDGKCIYSSDHQVSPNQKAFILTNEAGTYTMSHTDTVACYITDDDGNHFQVMEDGQISVLKLSSAS------SSQEKFEEEEGGATSRF---------------KHKHHCPRLFLLHADGSGTEFLSSHCVEKWLDEAYSDPTVALLKEPLPNQPDAFGITILKPSPQSVWSQWLLGKQVPDVTPPNLKNRSWHDFPGMEKRSPGPSFGTNIGVSLTLREKKAGPGALLQPMRSCPK-----VLEMRELREHRAFTKPLRDAVDSRLKKFIESLMEGEQLSEERKVKDPRTEEEQ 1616 HSP 3 Score: 64.6994 bits (156), Expect = 1.237e-9 Identity = 35/115 (30.43%), Postives = 61/115 (53.04%), Query Frame = 2
Query: 2180 FINNQNDEGFQEILLDY-LSNLRKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEA 2521
+N ++ EG ++ L L R+R+ ++ + E + VL Q++ + + +D + D + HNP +PD + WN LH++V FR YL+ V D ITDW R++E+
Sbjct: 637 LLNLKSKEGSSQLDLSSDLQRCRRRSLNDRHYTEHHSADVLQQVLLLASEEYCCLDAFGGTHNDTLYIFCHNPMNPDRQCQEF-WNVALHTDVKFRKYLEHVADTITDWTREEES 750
BLAST of Sperm associated antigen 17 vs. Ensembl Medaka
Match: ENSORLT00000006642.2 (sperm associated antigen 17 [Source:NCBI gene;Acc:101170055]) HSP 1 Score: 82.8037 bits (203), Expect = 4.563e-15 Identity = 71/282 (25.18%), Postives = 135/282 (47.87%), Query Frame = 2
Query: 503 KSSGGKKGSQVPGKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYE----MQIENLKSFWTNINPVLNDNKTLLNTPIITLVA-KHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPVKTSVAQTHENTTPRESIKEGVQASPDNTDSHMNFTFDKFNID--YYNFLLQDVPIESTSIYLQMSAMLDQVEIT 1327
K+ K ++ P K P +++ KL +RG+ +P YIDDEPD G Y ++ G LL+A+L IG +++K+ D E +++ + S+ + + + E +Q L FW+ + VL+ L ++ + +V +++ ++ + +++ +FE +A L+YD L +R+ ++Y +K +L+ +P + P + SP NT + + +D YYN LL +P E+ S+ L + ML+QV I+
Sbjct: 41 KTRASDKRAKTPSK--PVTSVMEKKTKLLRRGEVEPPT-YIDDEPDWGPQHYVLLVGFYQPLLIAALDGIGVHVANVIKLCCD-----RTQIRRKEQEQQSCDDSEPSQAASAISEAELAVQATKLDLFWSGMRAVLDSG--LPHSRLHDVVPLSYIVQDFHTQDPNDRMELGSRIFEGVANLIYDCLQWRREHQHYRNKLQLLHVPSISGSGYKPAEDAPTSPFQ-----SPINTKKPVCEESQRNQVDMCYYNSLLDLIPPEACSVPLMLHCMLEQVVIS 307 HSP 2 Score: 78.9518 bits (193), Expect = 6.193e-14 Identity = 49/160 (30.63%), Postives = 85/160 (53.12%), Query Frame = 2
Query: 6002 SNINLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIANNLEKNSSNVSLLEHFLQITTNTDQLIIPIVGRVM 6481
S + LPN + + + PN + VE+PVRRK T SL ++ I+ RG + P VD G+++ G T + + N G ++ F +KQP +TG+ V + P+AAGL +L + ++A+++ L + S + + + I T TD +P+ V+
Sbjct: 1728 SRVRLPNCIMSSKARRVPNQNFLTVEEPVRRKCRTASL---ADPRAII----RGFQLLPWSVDFGVVQAGTLSTAAVLMKNVGVDTCRFHVKQPPLATGLRVLYNSRPVAAGLQTELKVQLFAMSSLLAETESKKYISQDII-ILTETDIFYLPVTAIVV 1879 HSP 3 Score: 68.9366 bits (167), Expect = 6.954e-11 Identity = 69/348 (19.83%), Postives = 142/348 (40.80%), Query Frame = 2
Query: 4376 ELLLIESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDGEIIFTASVN---SQMSYLQPDGPFSYVFNPTSNVICEIIDSEGTEFIVNINGEFDV-KINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQKYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILH--SDSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIYSNPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVNQSDEEKK 5401
+++++E + V++ + + N+ G + S ++ + DG+ I+++ +Q +++ + +Y + T V C I D +G F V +G+ V K++ + +SQ +++ G + K+ H PRLF ++ DGSG+E L + L + D +E + N P TIL S W V P L R FP E ++ GP FG ++G L + + +P +S + ++ R+ + K +R + +L + + +++ QL ++ ++ + EE++
Sbjct: 1203 QVVMVEKEGQATVVMHPQRHTAQAFLADGTVVTGNNQGEYEVFPSSAGFLQIQRDGKCIYSSDHQVSPNQKAFILTNEAGTYTMSHTDTVACYITDDDGNHFQVMEDGQISVLKLSSAS------SSQEKFEEEEGGATSRF---------------KHKHHCPRLFLLHADGSGTEFLSSHCVEKWLDEAYSDPTVALLKEPLPNQPDAFGITILKPSPQSVWSQWLLGKQVPDVTPPNLKNRSWHDFPGMEKRSPGPSFGTNIGVSLTLREKKAGPGALLQPMRSCPK-----VLEMRELREHRAFTKPLRDAVDSRLKKFIESLMEGEQLSEERKVKDPRTEEEQ 1524 HSP 4 Score: 64.6994 bits (156), Expect = 1.331e-9 Identity = 35/115 (30.43%), Postives = 61/115 (53.04%), Query Frame = 2
Query: 2180 FINNQNDEGFQEILLDY-LSNLRKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEA 2521
+N ++ EG ++ L L R+R+ ++ + E + VL Q++ + + +D + D + HNP +PD + WN LH++V FR YL+ V D ITDW R++E+
Sbjct: 545 LLNLKSKEGSSQLDLSSDLQRCRRRSLNDRHYTEHHSADVLQQVLLLASEEYCCLDAFGGTHNDTLYIFCHNPMNPDRQCQEF-WNVALHTDVKFRKYLEHVADTITDWTREEES 658
BLAST of Sperm associated antigen 17 vs. Planmine SMEST
Match: SMESG000038878.1 (SMESG000038878.1) HSP 1 Score: 4072.7 bits (10561), Expect = 0.000e+0 Identity = 2156/2168 (99.45%), Postives = 2161/2168 (99.68%), Query Frame = 2
Query: 23 MSKKRNKSPAANIASGNKWESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEAPLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNXXXXXXXXXXXXXTDKSERAKSSGGKKGSQVPGKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPXXXXXXXXXXXXRISNGSDKENSDNNLYEMQIENLKSFWTNINPVLNDNKTLLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPVKTSVAQTHENTTPRESIKEGVQASPDNTDSHMNFTFDKFNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTPIWELNKSDLPKDLNEDSIDVFKKITDNIYFPXXXXXXXXXXXXIPLDDDDENRNKDQNSPIHVLDSITQRDREINTKNFGKNGALGHELQMLVASFRHFDLLKFIPTDDQISRKRAAKLQELINYLNNTGLSAIEINYAIKLFILENLTLSGVDENGVIRINNEDGYFQRNNYQVWDDPYMILNDVAQSIIDEENKTLKXXXXXXXXXXXXXXXXXPALSFIKNENFTEGILKXXXXXXXXXXPENIKSRRKSSVHFLETERVESPTMSTETQMDIMAFINNQNDEGFQEILLDYLSNLRKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEAIYQANLLNQELQIVETEIKETEVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTQELSLIVPGSLKAQKLXXXXXXXXXXXXXXXXXXXRQLSAQKKTEKDLAKNVEKTKDSKRPXXXXXXXXXXXXXXFEDSGENFDKENYQEIYTDRIDGEEYWPFISYHLDNDLAHFMGMVAHLFPSDGSIIRVFKMQKLLDTTSVRVSVITTQSHVFTIHILDPTYSPDSQIDNFDQILEQFSHSKNICNYGASTCTFNDGITIAASNNGYSGSQLKNKSPIGAPNDEISSHRSNDLTVNGNKEEIFQNDTNETNEKIITQKNPFVNHMDYIDSPNIILTTPDGVQVSVGYESAENENQRNLLLFMCKFESSLKPSVPPELSSIQAPEIGRYITSDGRVVTIFKNELIKVLFPDGTVAXXXXXXXXXXXXDDEVEEFKIENINVKESPNSKRKTKQSKSISVEPQTPLNTVLVEDSKPSCNYSRKWTITLSNGEVFETVANLNSIFNPKPLIETIETKLDDEATTDENNDILEANQPTEKERSFKSLQTKERLLTSYATDPSTGETLVTRSDGTLLLVSQDAGIFLCEHSDGTRFTKYFNVEKSSSSTYEPGEISRAKISELLLIESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDGEIIFTASVNSQMSYLQPDGPFSYVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVQKYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILHSDSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIYSNPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVNQSDEEKKIADEILEKFKVLKFSKESIANAIGSLVNINSTQGEMSSKAFETNAKTALPKNPSHRKLDVILEQXXXXXXXXXXXRASLKNHIVPKYFTSIYGKDFISKQAMDLQSLNKSMKLFPISREGTDVNDLKDKNEFLNETETSEVSLLSPKFQVDYLEQSPENSTKNSKNLLKPKLIIQKRATNYLYYDSNLFIPNEPKRLPELSNINLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIANNLEKNSSNVSLLEHFLQITTNTDQLIIPIVGRVMTAENYQSIYPISKTD 6526
MSKKRNKSPAANIASGNKWESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEAPLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNKKVDGKVSKAKKATDKSERAKSSGGKKGSQVPGKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPNENLNEEVENQERI NGSDKENSDNNLYEMQIENLKSFWTNINP+LNDNKTLLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPVKTSVAQTHENTTPRESIKEGVQASPDNTDSHMNFTFDKFNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTPIWELNKSDLPKDLNEDSIDVFKKITDNIY PKESLLELKTSLKIPLDDDDENRNKDQN+PIHVLDSITQRDREINTKNFGKNGALGHELQMLVASFRHFDLLKFIPTDDQISRKRAAKLQELINYLNNTGLSAIEINYAIKLFILENLTLSGVDENGVIRINNEDGYFQRNNYQVWDDPYMILNDVAQSIIDEENKTLKDEAASSEKDSISIIESEPALSFIKNENFTEGILKSSRVSSRSQSPENIKSRRKSSVHFLETERVESPTMSTETQMDIMAFINNQNDEGFQEILLDYLSNLRKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEAIYQANLLNQELQIVETEIKETEVKGKSSKTNEKGKKPKPSKSRSRSTSRGSSAEETSTQELSLIVPGSLKAQKLEQEKINKAKAEEEMKKEQKRQLSAQKKTEKDLAKNVEKTKDSKRPSSKASNKSKTKKTAFEDSGENFDKENYQEIYTDRIDGEEYWPFISYHLDNDLAHFMGMVAHLFPSDGSIIRVFKMQKLLDTTSVRVSVITTQSHVFTIHILDPTYSPDSQIDNFDQILEQFSHSKNICNYGASTCTFNDGITIAASNNGYSGSQLKNKSPIGAPNDEISSHRSNDLTVNGNKEEIFQNDTNETNEKIITQKNPFVNHMDYIDSPNIILTTPDGVQVSVGYESAENEN RNLLLFMCKFESSLKPSVPPELSSIQAPEIGRYITSDGRVVTIFKNELIKVLFPDGTVALSQLSMQSSVSQDDEVEEFKIENINVKESPNSKRKTKQSKSISVEPQTPLNTV VEDSKPS NYSRKWTITLSNGEVFETVANLNSIFNPKPLIETIETKLDDEATTDENNDILEANQPTEKERSFKSLQTKERLLTSYATDPSTGETLVTRSDGTLLLVSQDAGIFLCEHSDGTRFTKYFNVEKSSSSTYEPGEISRAKISELLLIESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDGEIIFTASVNSQMSYLQPDGPFSYVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNV KYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILHSDSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEI+SNPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVNQS+EEKKIADEILEKFKVLKFSKESIANAIGSLVNINSTQGEMSSKAFETNAKTAL KNPSHRKLDVILEQEKINKEKEIENRASLKNHIVPKYFTSIYGKDFISKQAMDLQSLNKSMKLFPISREGTDVNDLK+KNEFLNETETSEVSLLSPKFQVDYLEQSPENSTKNSKNLLKPKLIIQKRATNYLYYDSNLFIPNEPKRLPELSNINLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIANNLEKNSSNVSLLEHFLQITTNTDQLIIPIVGRVMTAENYQSIYPISKTD
Sbjct: 1 MSKKRNKSPAANIASGNKWESNITAAELNEDNWSYSLSMIIENIPQDTETINYINLGISTGLRKKFTLITLEKITQLINDFGNPKSKKGKEAPLNFEICEIGKSHLDKNEALPAPYLAKFIKWIMLTNKTEDMKNTGDTNKKVDGKVSKAKKATDKSERAKSSGGKKGSQVPGKRSPEPPSVKQDNKLRKRGDPDPDIQYIDDEPDDGSSRYFVISGLVDHLLLASLADIGCITTSIVKVTRDIHIPNENLNEEVENQERIPNGSDKENSDNNLYEMQIENLKSFWTNINPILNDNKTLLNTPIITLVAKHLMFPVERENEDLKIKCAMELFESLAVLMYDLLDLKRQWKNYLDKSKLIALPVKTSVAQTHENTTPRESIKEGVQASPDNTDSHMNFTFDKFNIDYYNFLLQDVPIESTSIYLQMSAMLDQVEITAGEKTPIWELNKSDLPKDLNEDSIDVFKKITDNIYLPKESLLELKTSLKIPLDDDDENRNKDQNAPIHVLDSITQRDREINTKNFGKNGALGHELQMLVASFRHFDLLKFIPTDDQISRKRAAKLQELINYLNNTGLSAIEINYAIKLFILENLTLSGVDENGVIRINNEDGYFQRNNYQVWDDPYMILNDVAQSIIDEENKTLKDEAASSEKDSISIIESEPALSFIKNENFTEGILKSSRVSSRSQSPENIKSRRKSSVHFLETERVESPTMSTETQMDIMAFINNQNDEGFQEILLDYLSNLRKRNFSEWCFEEFYEPHVLLQIVHQSIYKLPYIDIYDQRRVDGKLVVLHNPFHPDTKYNHHSWNKWLHSNVGFRNYLDFVHDKITDWNRDQEAIYQANLLNQELQIVETEIKETEVKGKSSKTNEKGKKPKPSKSRSRSTSRGSSAEETSTQELSLIVPGSLKAQKLEQEKINKAKAEEEMKKEQKRQLSAQKKTEKDLAKNVEKTKDSKRPSSKASNKSKTKKTAFEDSGENFDKENYQEIYTDRIDGEEYWPFISYHLDNDLAHFMGMVAHLFPSDGSIIRVFKMQKLLDTTSVRVSVITTQSHVFTIHILDPTYSPDSQIDNFDQILEQFSHSKNICNYGASTCTFNDGITIAASNNGYSGSQLKNKSPIGAPNDEISSHRSNDLTVNGNKEEIFQNDTNETNEKIITQKNPFVNHMDYIDSPNIILTTPDGVQVSVGYESAENENHRNLLLFMCKFESSLKPSVPPELSSIQAPEIGRYITSDGRVVTIFKNELIKVLFPDGTVALSQLSMQSSVSQDDEVEEFKIENINVKESPNSKRKTKQSKSISVEPQTPLNTVPVEDSKPSFNYSRKWTITLSNGEVFETVANLNSIFNPKPLIETIETKLDDEATTDENNDILEANQPTEKERSFKSLQTKERLLTSYATDPSTGETLVTRSDGTLLLVSQDAGIFLCEHSDGTRFTKYFNVEKSSSSTYEPGEISRAKISELLLIESKQYPAVIIDLTNEKIETRCGSMGALLSNSCGHHIFKHSDNTEIEYRPDGEIIFTASVNSQMSYLQPDGPFSYVFNPTSNVICEIIDSEGTEFIVNINGEFDVKINKKNDKPFEEASQVLETDQDLGKLRKLNSVISMTNDSNDNVLKYDQHAPRLFTINPDGSGSELLRYRDIVDTLVNSDIDENYFHCREEIQNSPKIIAHTILHSDSKIKPWKYEYSRKTVLPKGLTCRDMKTFPPKEIKTDGPPFGMSVGKGLEIFSNPIMAKNVQKPKKSFRQDHWLNPMQFRQFYHYPTIGKDVRSSFSEKLIDYNKKILQQRQLYKDLEIVNQSEEEKKIADEILEKFKVLKFSKESIANAIGSLVNINSTQGEMSSKAFETNAKTALHKNPSHRKLDVILEQEKINKEKEIENRASLKNHIVPKYFTSIYGKDFISKQAMDLQSLNKSMKLFPISREGTDVNDLKEKNEFLNETETSEVSLLSPKFQVDYLEQSPENSTKNSKNLLKPKLIIQKRATNYLYYDSNLFIPNEPKRLPELSNINLPNYLYAERPSIEPNIHQALVEDPVRRKVYTTSLIGGSETSQILQRKTRGLNVFPARVDTGLLKEGLNYTFKIKLINTGPESSCFKIKQPHSSTGIWVEFLPGPIAAGLSRKLNIHIYAIANNLEKNSSNVSLLEHFLQITTNTDQLIIPIVGRVMTAENYQSIYPISKTD 2168 The following BLAST results are available for this feature:
BLAST of Sperm associated antigen 17 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 2
BLAST of Sperm associated antigen 17 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Sperm associated antigen 17 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Sperm associated antigen 17 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of Sperm associated antigen 17 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of Sperm associated antigen 17 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 1
BLAST of Sperm associated antigen 17 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 2
BLAST of Sperm associated antigen 17 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Sperm associated antigen 17 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of Sperm associated antigen 17 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of Sperm associated antigen 17 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Sperm associated antigen 17 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 4
BLAST of Sperm associated antigen 17 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 2
BLAST of Sperm associated antigen 17 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30035047 ID=SMED30035047|Name=Sperm associated antigen 17|organism=Schmidtea mediterranea sexual|type=transcript|length=6693bpback to top protein sequence of SMED30035047-orf-1 >SMED30035047-orf-1 ID=SMED30035047-orf-1|Name=SMED30035047-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=2193bp MSKKRNKSPAANIASGNKWESNITAAELNEDNWSYSLSMIIENIPQDTETback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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