Phospholipase A(2)

Overview
NamePhospholipase A(2)
Smed IDSMED30035032
Length (bp)626
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Phospholipase A(2) (SMED30035032) t-SNE clustered cells

Violin plots show distribution of expression levels for Phospholipase A(2) (SMED30035032) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Phospholipase A(2) (SMED30035032) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Phospholipase A(2) (SMED30035032) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30035032

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30035032h1SMcG0018756 Contig52954newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30035032h1SMcG0018756 Contig52954uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30035032h1SMcG0018756 dd_Smed_v4_9181_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30035032h1SMcG0018756 dd_Smed_v4_9181_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30035032h1SMcG0018756 dd_Smed_v4_9181_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
prepharyngeal regionSMED30035032h1SMcG0018756 dd_Smed_v6_9181_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30035032h1SMcG0018756 OX_Smed_1.0.09283ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Phospholipase A(2) vs. Ensembl Human
Match: PLA2G2A (phospholipase A2 group IIA [Source:HGNC Symbol;Acc:HGNC:9031])

HSP 1 Score: 66.6254 bits (161), Expect = 1.693e-13
Identity = 39/119 (32.77%), Postives = 57/119 (47.90%), Query Frame = 2
Query:  113 SNAGLIEFNEMIRKYVKINPGKLVG-----YGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESH 454
            ++  L+ F+ MI    K+  GK        YG +CG G     P D  DRCC  HD CYK  +++    K  FL+YK+    ++ITC       ++LC CDK    C   N   +N+ +
Sbjct:   18 AHGNLVNFHRMI----KLTTGKEAALSYGFYGCHCGVGGRG-SPKDATDRCCVTHDCCYKRLEKRGCGTK--FLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKY 129          
BLAST of Phospholipase A(2) vs. Ensembl Human
Match: PLA2G2A (phospholipase A2 group IIA [Source:HGNC Symbol;Acc:HGNC:9031])

HSP 1 Score: 66.6254 bits (161), Expect = 1.693e-13
Identity = 39/119 (32.77%), Postives = 57/119 (47.90%), Query Frame = 2
Query:  113 SNAGLIEFNEMIRKYVKINPGKLVG-----YGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESH 454
            ++  L+ F+ MI    K+  GK        YG +CG G     P D  DRCC  HD CYK  +++    K  FL+YK+    ++ITC       ++LC CDK    C   N   +N+ +
Sbjct:   18 AHGNLVNFHRMI----KLTTGKEAALSYGFYGCHCGVGGRG-SPKDATDRCCVTHDCCYKRLEKRGCGTK--FLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKY 129          
BLAST of Phospholipase A(2) vs. Ensembl Human
Match: PLA2G2A (phospholipase A2 group IIA [Source:HGNC Symbol;Acc:HGNC:9031])

HSP 1 Score: 63.1586 bits (152), Expect = 1.738e-12
Identity = 35/104 (33.65%), Postives = 50/104 (48.08%), Query Frame = 2
Query:  158 VKINPGKLVG-----YGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESH 454
            +K+  GK        YG +CG G     P D  DRCC  HD CYK  +++    K  FL+YK+    ++ITC       ++LC CDK    C   N   +N+ +
Sbjct:    2 IKLTTGKEAALSYGFYGCHCGVGGRG-SPKDATDRCCVTHDCCYKRLEKRGCGTK--FLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKY 102          
BLAST of Phospholipase A(2) vs. Ensembl Human
Match: PLA2G5 (phospholipase A2 group V [Source:HGNC Symbol;Acc:HGNC:9038])

HSP 1 Score: 58.5362 bits (140), Expect = 1.267e-10
Identity = 40/131 (30.53%), Postives = 58/131 (44.27%), Query Frame = 2
Query:   65 NIVFFMWIYFICFPCLSNAGLIEFNEMIRKYVKINPGKLVG-YGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESH 454
             ++   W      P +   GL++   MI K    N     G YG YCG G     P DG D CC  HD CY   +EK  + +T   +YK+      +TC+        LC CD+ +  C+K N + +N  +
Sbjct:    3 GLLPLAWFLACSVPAV-QGGLLDLKSMIEKVTGKNALTNYGFYGCYCGWGGRGT-PKDGTDWCCWAHDHCYGRLEEKGCNIRTQ--SYKYRFAWGVVTCEPGPFCHVNLCACDRKLVYCLKRNLRSYNPQY 129          
BLAST of Phospholipase A(2) vs. Ensembl Human
Match: PLA2G2D (phospholipase A2 group IID [Source:HGNC Symbol;Acc:HGNC:9033])

HSP 1 Score: 55.4546 bits (132), Expect = 2.413e-9
Identity = 36/113 (31.86%), Postives = 52/113 (46.02%), Query Frame = 2
Query:  116 NAGLIEFNEMIRKYVKINP-GKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQ-TETELCLCDKVINICIKENWKDFNE 448
              G++  N+M+++     P      YG +CG G    +P D  D CCQ HD CY   K +  S   Y   Y++      I C D G   E +LC CDK +  C+K N   + +
Sbjct:   19 QGGILNLNKMVKQVTGKMPILSYWPYGCHCGLGGRG-QPKDATDWCCQTHDCCYDHLKTQGCS--IYKDYYRYNFSQGNIHCSDKGSWCEQQLCACDKEVAFCLKRNLDTYQK 128          
BLAST of Phospholipase A(2) vs. Ensembl Celegans
Match: Y69A2AL.2 (Phospholipase A(2) [Source:UniProtKB/TrEMBL;Acc:Q3V5J7])

HSP 1 Score: 57.7658 bits (138), Expect = 1.495e-10
Identity = 38/100 (38.00%), Postives = 53/100 (53.00%), Query Frame = 2
Query:  188 YGNYCGAGNIPVEPLDGIDRCCQKHDLCYK---LWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCD-KVINICIKENWKDFNE--SHYVRDP 469
            YG  C +G  P +P+DGIDRCCQ H+ CY    L K+  +S+  YF  YKWE    Q  C +  +    +C CD + IN   K  +  F++   +  RDP
Sbjct:   41 YGCSC-SGISPNKPIDGIDRCCQVHNDCYNELLLTKKCQNSNSPYFCLYKWECVYQQPACNNESKCTQSVCQCDEQFINCLAKYPYPTFSKKCQYSERDP 139          
BLAST of Phospholipase A(2) vs. Ensembl Celegans
Match: C07E3.9 (Phospholipase A(2) [Source:UniProtKB/TrEMBL;Acc:Q17790])

HSP 1 Score: 46.595 bits (109), Expect = 1.696e-6
Identity = 34/111 (30.63%), Postives = 45/111 (40.54%), Query Frame = 2
Query:  122 GLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEK---FHSDKTYFLAYKWEVEDNQITCKDVGQ-TETELCLCDKVINICIKENWKDF 442
             L    E+    +  N      YG +CG G    EP+DGID CC  HD CY    +       +  Y   Y W   ++   C D     +  LC CDK++  C    WK F
Sbjct:   25 ALWNLEEVAECELHYNALHYNNYGCWCGIGG-SHEPVDGIDECCMHHDKCYDAAVDNKVCMDVEIEYVDDYSWSCMNSTAICSDKNMGCKAALCDCDKIVVEC----WKKF 130          
BLAST of Phospholipase A(2) vs. Ensembl Zebrafish
Match: zgc:92162 (zgc:92162 [Source:ZFIN;Acc:ZDB-GENE-040718-41])

HSP 1 Score: 50.447 bits (119), Expect = 9.367e-8
Identity = 37/119 (31.09%), Postives = 50/119 (42.02%), Query Frame = 2
Query:  113 SNAGLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVG-QTETELCLCDKVINICIKEN--------WKDF 442
            S  GL+E   +I+     +    V YG YCG G     P D  D CC KHD CY     +F   +T    Y W  +D Q  C  +  +    LC CD+    C+++         W DF
Sbjct:   26 SKRGLLELAGVIKCSTGRSALSYVMYGCYCGLGG-QGWPRDRADWCCHKHDCCYG--DAEFAGCQTKTDRYHWTCDDEQADCDSLNDRCAKILCRCDREAARCLRKAPFNPKYALWPDF 141          
BLAST of Phospholipase A(2) vs. Ensembl Xenopus
Match: ENSXETT00000035968.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:4:7950736:7951885:1 gene:ENSXETG00000016760.1 transcript:ENSXETT00000035968.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.7658 bits (138), Expect = 3.201e-10
Identity = 40/126 (31.75%), Postives = 58/126 (46.03%), Query Frame = 2
Query:  149 RKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESHYVRDP---KQRKLVTSLNELLSAI 517
            R  V+I+   +  YG +CG G     P D +DRCC  HD CY+     F+ D T F AY++E   ++I CK     +   C CDK    C+           + RDP   K   L   ++  ++AI
Sbjct:   17 RDKVQISLFAINLYGCFCGIGG-SGHPQDEVDRCCFLHDCCYQFTNVTFNCDST-FQAYEYECARSRIKCKSSSVCDQMTCECDKKFAECLTAAKPVDEHVLFNRDPACNKPTDLCPPVHPSVAAI 140          
BLAST of Phospholipase A(2) vs. Ensembl Xenopus
Match: ENSXETT00000024628.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:9:31942973:31943890:-1 gene:ENSXETG00000015087.1 transcript:ENSXETT00000024628.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.0694 bits (131), Expect = 2.221e-9
Identity = 30/84 (35.71%), Postives = 39/84 (46.43%), Query Frame = 2
Query:  110 LSNAGLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCK 361
            L   GL++    IR     +  + +GYG +CG G     P+D  DRCC  HD CYK  K K H  +     YKW   D    C+
Sbjct:    8 LQKRGLMQLAAAIRCVTGRSSLRYLGYGCHCGPGGRG-GPIDATDRCCHDHDCCYK--KAKKHRCQPKIGGYKWSCRDRTFQCR 88          
BLAST of Phospholipase A(2) vs. Ensembl Xenopus
Match: eml6 (echinoderm microtubule associated protein like 6 [Source:Xenbase;Acc:XB-GENE-6039541])

HSP 1 Score: 54.6842 bits (130), Expect = 3.956e-9
Identity = 38/118 (32.20%), Postives = 53/118 (44.92%), Query Frame = 2
Query:  110 LSNAGLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQ-TETELCLCDKVINICIKENWKDFNESHYV 460
            L   GLI+    I+     +    +GYG +CG G   V P D  D CC  HD CY+   EK+   KT    Y W  +DN + C D+    +  +C CD     C+ +    FN  H +
Sbjct:   12 LQRRGLIQLAGTIQCGTGRSAVHYIGYGCHCGLGGQGV-PKDNTDWCCHSHDCCYEF-AEKYGC-KTKLGQYSWTCKDNTVKCGDMKDWCQKIVCKCDSKFAECLSKT--RFNSKHIL 124          
BLAST of Phospholipase A(2) vs. Ensembl Xenopus
Match: eml6 (echinoderm microtubule associated protein like 6 [Source:Xenbase;Acc:XB-GENE-6039541])

HSP 1 Score: 53.9138 bits (128), Expect = 7.739e-9
Identity = 39/132 (29.55%), Postives = 59/132 (44.70%), Query Frame = 2
Query:   68 IVFFMWIYFICFPCLSNAGLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQ-TETELCLCDKVINICIKENWKDFNESHYV 460
            I  F++++      +   GLI+    I+     +    +GYG +CG G   V P D  D CC  HD CY+   EK+   KT    Y W  +DN + C D+    +  +C CD     C+ +    FN  H +
Sbjct:   10 ITAFLYVH------VERRGLIQLAGTIQCGTGRSAVHYIGYGCHCGLGGQGV-PKDNTDWCCHSHDCCYEF-AEKYGC-KTKLGQYSWTCKDNTVKCGDMKDWCQKIVCKCDSKFAECLSKT--RFNSKHIL 130          
BLAST of Phospholipase A(2) vs. Ensembl Xenopus
Match: eml6 (echinoderm microtubule associated protein like 6 [Source:Xenbase;Acc:XB-GENE-6039541])

HSP 1 Score: 54.299 bits (129), Expect = 8.172e-9
Identity = 38/118 (32.20%), Postives = 54/118 (45.76%), Query Frame = 2
Query:  110 LSNAGLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQ-TETELCLCDKVINICIKENWKDFNESHYV 460
            L   GLI+    I+     +    +GYG +CG G   V P D  D CC  HD CY+ + EK+   KT    Y W  +DN + C D+    +  +C CD     C+ +    FN  H +
Sbjct:   28 LQRRGLIQLAGTIQCGTGRSAVHYIGYGCHCGLGGQGV-PKDNTDWCCHSHDCCYE-FAEKY-GCKTKLGQYSWTCKDNTVKCGDMKDWCQKIVCKCDSKFAECLSKT--RFNSKHIL 140          
BLAST of Phospholipase A(2) vs. Ensembl Mouse
Match: Pla2g2d (phospholipase A2, group IID [Source:MGI Symbol;Acc:MGI:1341796])

HSP 1 Score: 61.2326 bits (147), Expect = 1.155e-11
Identity = 39/114 (34.21%), Postives = 55/114 (48.25%), Query Frame = 2
Query:  113 SNAGLIEFNEMIRKYV-KINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQ-TETELCLCDKVINICIKENWKDFNE 448
            +  GL+  N+M+     K        YG +CG G    +P D  D CCQKHD CY     K    K+    YK+ +    I C D G   E +LC CDK + +C+K+N   +N+
Sbjct:   17 TQGGLLNLNKMVTHMTGKKAFFSYWPYGCHCGLGG-KGQPKDATDWCCQKHDCCYA--HLKIDGCKSLTDNYKYSISQGTIQCSDNGSWCERQLCACDKEVALCLKQNLDSYNK 127          
BLAST of Phospholipase A(2) vs. Ensembl Mouse
Match: Pla2g5 (phospholipase A2, group V [Source:MGI Symbol;Acc:MGI:101899])

HSP 1 Score: 59.6918 bits (143), Expect = 3.483e-11
Identity = 40/131 (30.53%), Postives = 59/131 (45.04%), Query Frame = 2
Query:   65 NIVFFMWIYFICFPCLSNAGLIEFNEMIRKYVKINPGKLVG-YGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESH 454
             ++   W      P +   GL+E   MI K  + N  K  G YG YCG G     P DG D CCQ HD CY   +EK  + +T   +Y +   +  + C+        LC CD+ +  C++ N   +N  +
Sbjct:    3 GLLTLAWFLACSVPAVP-GGLLELKSMIEKVTRKNAFKNYGFYGCYCGWGGRGT-PKDGTDWCCQMHDRCYGQLEEKDCAIRT--QSYDYRYTNGLVICEHDSFCPMRLCACDRKLVYCLRRNLWTYNPLY 129          
BLAST of Phospholipase A(2) vs. Ensembl Mouse
Match: Pla2g5 (phospholipase A2, group V [Source:MGI Symbol;Acc:MGI:101899])

HSP 1 Score: 59.6918 bits (143), Expect = 3.483e-11
Identity = 40/131 (30.53%), Postives = 59/131 (45.04%), Query Frame = 2
Query:   65 NIVFFMWIYFICFPCLSNAGLIEFNEMIRKYVKINPGKLVG-YGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESH 454
             ++   W      P +   GL+E   MI K  + N  K  G YG YCG G     P DG D CCQ HD CY   +EK  + +T   +Y +   +  + C+        LC CD+ +  C++ N   +N  +
Sbjct:    3 GLLTLAWFLACSVPAVP-GGLLELKSMIEKVTRKNAFKNYGFYGCYCGWGGRGT-PKDGTDWCCQMHDRCYGQLEEKDCAIRT--QSYDYRYTNGLVICEHDSFCPMRLCACDRKLVYCLRRNLWTYNPLY 129          
BLAST of Phospholipase A(2) vs. Ensembl Mouse
Match: Pla2g5 (phospholipase A2, group V [Source:MGI Symbol;Acc:MGI:101899])

HSP 1 Score: 59.6918 bits (143), Expect = 3.483e-11
Identity = 40/131 (30.53%), Postives = 59/131 (45.04%), Query Frame = 2
Query:   65 NIVFFMWIYFICFPCLSNAGLIEFNEMIRKYVKINPGKLVG-YGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESH 454
             ++   W      P +   GL+E   MI K  + N  K  G YG YCG G     P DG D CCQ HD CY   +EK  + +T   +Y +   +  + C+        LC CD+ +  C++ N   +N  +
Sbjct:    3 GLLTLAWFLACSVPAVP-GGLLELKSMIEKVTRKNAFKNYGFYGCYCGWGGRGT-PKDGTDWCCQMHDRCYGQLEEKDCAIRT--QSYDYRYTNGLVICEHDSFCPMRLCACDRKLVYCLRRNLWTYNPLY 129          
BLAST of Phospholipase A(2) vs. Ensembl Mouse
Match: Pla2g5 (phospholipase A2, group V [Source:MGI Symbol;Acc:MGI:101899])

HSP 1 Score: 59.6918 bits (143), Expect = 3.483e-11
Identity = 40/131 (30.53%), Postives = 59/131 (45.04%), Query Frame = 2
Query:   65 NIVFFMWIYFICFPCLSNAGLIEFNEMIRKYVKINPGKLVG-YGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESH 454
             ++   W      P +   GL+E   MI K  + N  K  G YG YCG G     P DG D CCQ HD CY   +EK  + +T   +Y +   +  + C+        LC CD+ +  C++ N   +N  +
Sbjct:    3 GLLTLAWFLACSVPAVP-GGLLELKSMIEKVTRKNAFKNYGFYGCYCGWGGRGT-PKDGTDWCCQMHDRCYGQLEEKDCAIRT--QSYDYRYTNGLVICEHDSFCPMRLCACDRKLVYCLRRNLWTYNPLY 129          
BLAST of Phospholipase A(2) vs. UniProt/SwissProt
Match: sp|P0DMT3|PA2HS_ECHCO (Phospholipase A2 homolog ECO_00035 OS=Echis coloratus OX=64175 PE=1 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.478e-14
Identity = 39/113 (34.51%), Postives = 59/113 (52.21%), Query Frame = 2
Query:  122 GLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESHYV 460
             +IE  +MI +     P   V YG +CG G+   +P D  DRCC  H  CY    +   S KT    YKWE  + +I C++    +  +C CDK + IC++EN K +N+ + +
Sbjct:    1 SVIELGKMIVQLTNKTPASYVSYGCFCGGGDRG-KPKDATDRCCFVHSCCYDTLPD--CSPKTDQYKYKWE--NGEIICENSTSCKKRICECDKAVAICLRENLKTYNKKYKI 108          
BLAST of Phospholipase A(2) vs. UniProt/SwissProt
Match: sp|P0DMT1|PA2HS_ECHPL (Phospholipase A2 homolog EPL_00195 OS=Echis pyramidum leakeyi OX=38415 PE=1 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.958e-13
Identity = 37/113 (32.74%), Postives = 58/113 (51.33%), Query Frame = 2
Query:  122 GLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESHYV 460
             +IE  +MI +     P   V YG +CG G+   +P D  DRCC  H  CY    +   S KT    YKWE  + +I C++    +  +C CDK + IC+++N   +N+ + +
Sbjct:    1 SVIELGKMIIQLTNKTPASYVSYGCFCGGGD-KGKPKDATDRCCFVHSCCYDTLPD--CSPKTDQYKYKWE--NGEIICENSTSCKKRICECDKAVAICLRDNLNTYNKKYRI 108          
BLAST of Phospholipase A(2) vs. UniProt/SwissProt
Match: sp|C1JAR9|PA2_SCOVI (Phospholipase A2 Scol/Pla OS=Scolopendra viridis OX=118503 PE=1 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 4.744e-13
Identity = 47/139 (33.81%), Postives = 64/139 (46.04%), Query Frame = 2
Query:   68 IVFFMWIYFICFPCLSNAGLIE----FNEMIRKYV--KINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEK---FHSDKTYFLAYKWEVEDNQITCKDVGQT-ETELCLCDKVINICIKENWKDFNESH 454
            I     I F  +   +N+  IE    +N     ++  K  P K  GYGN+CG G     P+D IDRCCQ HD CY    E     +     F+ Y W ++D QI C          +C CDK I  C+ +N KD+N  +
Sbjct:    5 IPLLFIISFGLYVSTTNSSPIERRSLWNFFFMTFIGGKRAPWKYDGYGNHCGIGG-KGSPVDSIDRCCQVHDRCYHEVNENECGSYKRNVKFIDYDWYMQDKQIVCDTTDTVCAQAICKCDKDIVECLNQNDKDYNPKY 142          
BLAST of Phospholipase A(2) vs. UniProt/SwissProt
Match: sp|P14555|PA2GA_HUMAN (Phospholipase A2, membrane associated OS=Homo sapiens OX=9606 GN=PLA2G2A PE=1 SV=2)

HSP 1 Score: 66.6254 bits (161), Expect = 8.130e-13
Identity = 39/119 (32.77%), Postives = 57/119 (47.90%), Query Frame = 2
Query:  113 SNAGLIEFNEMIRKYVKINPGKLVG-----YGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESH 454
            ++  L+ F+ MI    K+  GK        YG +CG G     P D  DRCC  HD CYK  +++    K  FL+YK+    ++ITC       ++LC CDK    C   N   +N+ +
Sbjct:   18 AHGNLVNFHRMI----KLTTGKEAALSYGFYGCHCGVGGRG-SPKDATDRCCVTHDCCYKRLEKRGCGTK--FLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKY 129          
BLAST of Phospholipase A(2) vs. UniProt/SwissProt
Match: sp|P0DPT7|PA21_SCODE (Phospholipase A2 SSD387 OS=Scolopendra dehaani OX=2609776 PE=1 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 8.764e-13
Identity = 43/114 (37.72%), Postives = 52/114 (45.61%), Query Frame = 2
Query:  125 LIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEK---FHSDKTYFLAYKWEVEDNQITCKDVGQT-ETELCLCDKVINICIKENWKDFNESH 454
            L  F  M     K +P +  GYGNYCG G     P+D IDRCCQ HD CY    E     +      + Y W +E  +I C          LC CDK I  C  EN KD+N  +
Sbjct:   30 LANFLSMTLTAAKRSPQEYDGYGNYCGWGGEGT-PVDSIDRCCQVHDNCYGTVNENECGHYIRNVKLIDYDWHMEGTEIVCDPKDDACGRALCKCDKDIIDCFNENDKDYNPEY 142          
BLAST of Phospholipase A(2) vs. TrEMBL
Match: A0A218UMK1 (Phospholipase A(2) OS=Lonchura striata domestica OX=299123 GN=PLA2G2E PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 3.918e-14
Identity = 42/129 (32.56%), Postives = 62/129 (48.06%), Query Frame = 2
Query:   68 IVFFMWIYFICFPCLSNAGLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESH 454
            ++  + + F C   L++  L +F  MI++    +P     YG YCG G    +PLD  DRCC  HD CYK       S KT    YK+  + NQITC +    + + C CDK    C +     + +S+
Sbjct:    3 VLLVLTMLFACRLALASCNLAQFAVMIKQKTGKSPLAYNRYGCYCGWGGSK-QPLDATDRCCHAHDCCYKKLAASGCSPKT--ATYKYVFQGNQITCGNGNSCQKQTCACDKKAVECFQRAASSYRKSY 128          
BLAST of Phospholipase A(2) vs. TrEMBL
Match: A0A0L8IC38 (Phospholipase A(2) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22024454mg PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 5.507e-14
Identity = 43/134 (32.09%), Postives = 64/134 (47.76%), Query Frame = 2
Query:   77 FMWIYFICFPCLSNAGLIE-----FNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCY----KLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNES 451
             + ++  CF C  + GL++     F EMI      NP    GYG +CG G    +P+D ID CC+ HD CY    ++        K YF +Y W      I C +  + +   C+CDK  +IC   +  ++NES
Sbjct:    5 LLVLFLGCFLCTVSDGLVKRSLDSFGEMIEAETGRNPFDFNGYGKWCGFGG-SGDPVDSIDNCCRNHDHCYERVNRVQCNSLTHTKVYFASYHWYKHQGLIYCLNSDECQQATCMCDKKASICFYNHLIEYNES 137          
BLAST of Phospholipase A(2) vs. TrEMBL
Match: A0A3M7SJW0 (Phospholipase A(2) OS=Brachionus plicatilis OX=10195 GN=BpHYR1_040149 PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 7.593e-14
Identity = 42/132 (31.82%), Postives = 69/132 (52.27%), Query Frame = 2
Query:   68 IVFFMWIYFICFPCL---SNAGLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVG-QTETELCLCDKVINICIKENWKDFNES 451
            ++F +W +F     L   ++  L++F  M+    + +     GYGNYCG G    +P+D ID CC+ HD CY+     F    TYF  Y +  ++NQI+C     + ++ +C CDK+   C K++   +N S
Sbjct:    4 LIFVIWTFFTFNSILAIPTSRNLLQFKSMVEYLTERSAFDFDGYGNYCGMGG-KGDPVDAIDNCCKNHDQCYRDTSNLFC--YTYFSTYDYSAKNNQISCSLTNTECQSGVCRCDKLAAECFKQHLSIYNPS 132          
BLAST of Phospholipase A(2) vs. TrEMBL
Match: A0A087SVA4 (Phospholipase A(2) (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_08523 PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 3.729e-13
Identity = 43/143 (30.07%), Postives = 72/143 (50.35%), Query Frame = 2
Query:   71 VFFMWIYFICFPCLSNA-------GLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESHYVRDPKQR 478
            V F+W+  + F  +S+         L++  +M      ++P   + YGN+CG G    +P+D ID CCQ HD CY   ++K  + + + + Y W++ +  I C D    E  +C CDK +  CI ++   ++E HY    K R
Sbjct:    8 VIFLWMS-LAFAYVSSGKLRRRQRDLLDLADMFDTLTGLDPTDYIPYGNWCGYGG-QGKPVDHIDSCCQIHDECYGQSEKKCSNVQVHVVQYAWKINNATIICSDEANCEASICKCDKEVVECIAQHSHTYSE-HYRFIRKSR 147          
BLAST of Phospholipase A(2) vs. TrEMBL
Match: A0A0A1WC82 (Phospholipase A2 Group IIA c OS=Echis coloratus OX=64175 PE=2 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 4.492e-13
Identity = 42/127 (33.07%), Postives = 66/127 (51.97%), Query Frame = 2
Query:   80 MWIYFICFPCLSNAGLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESHYV 460
            +WI  +   C+  + +IE  +MI +     P   V YG +CG G+   +P D  DRCC  H  CY    +   S KT    YKWE  + +I C++    +  +C CDK + IC++EN K +N+ + +
Sbjct:    4 LWIVAVWLMCVEGS-VIELGKMIVQLTNKTPASYVSYGCFCGGGDRG-KPKDATDRCCFVHSCCYDTLPD--CSPKTDQYKYKWE--NGEIICENSTSCKKRICECDKAVAICLRENLKTYNKKYKI 124          
BLAST of Phospholipase A(2) vs. Ensembl Cavefish
Match: zgc:92162 (phospholipase A2-like [Source:NCBI gene;Acc:103040974])

HSP 1 Score: 50.8322 bits (120), Expect = 6.764e-8
Identity = 36/119 (30.25%), Postives = 52/119 (43.70%), Query Frame = 2
Query:  113 SNAGLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDV-GQTETELCLCDKVINICIKEN--------WKDF 442
            S  GL+E   +I+     +    + YG YCG G     P D  D CC KHD CY     +F   +T    Y+W  E+ +  C  +  + E  LC CD+    C+++         W DF
Sbjct:   26 SKRGLLELAGVIKCSTGRSALAYMMYGCYCGLGGQGW-PRDRADWCCHKHDCCYG--DAEFEGCQTKTAKYQWTCENKEADCDSLEDKCEKILCRCDREAAKCLRKAPFNRQYALWPDF 141          
BLAST of Phospholipase A(2) vs. Ensembl Cavefish
Match: ENSAMXT00000000319.2 (phospholipase A2 [Source:NCBI gene;Acc:103024514])

HSP 1 Score: 47.7506 bits (112), Expect = 1.049e-6
Identity = 39/124 (31.45%), Postives = 57/124 (45.97%), Query Frame = 2
Query:  110 LSNAGLIEFNEMIRKYVKINPGKL--VGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEK------FHSDKTYFLAYKWEVEDNQITCK-DVGQTETELCLCDKVINICIKENWKDFNESH 454
            L    L +F +MI   +  +   L    YG YCG G     P+D +DRCC+ HD CY    +         +  T   AY  + E+ +ITCK +  + E  +C CD+    C   +  D+NE H
Sbjct:   32 LDYRALWQFRDMIICTIPDSWPALDYSDYGCYCGKGGSGT-PVDDLDRCCEVHDRCYSDAMQHNACWPILDNPYTEIYAYSCDKENKKITCKSNNNECEMFICECDRKAAECFAVS--DYNEEH 152          
BLAST of Phospholipase A(2) vs. Ensembl Cavefish
Match: ENSAMXT00000030049.1 (phospholipase A2-like [Source:NCBI gene;Acc:103028136])

HSP 1 Score: 46.595 bits (109), Expect = 1.381e-6
Identity = 33/115 (28.70%), Postives = 49/115 (42.61%), Query Frame = 2
Query:  122 GLIEFNEMIR-KYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSD--KTYFLAYKWEVEDNQITCKDV-GQTETELCLCDKVINICIKENWKDFNESH 454
             L +F  MI+     +NP     YG +CG G     P D +DRCCQ HD CY   K+         Y   Y     + + +C     + E  +C CD+V   C  +    +N ++
Sbjct:   12 ALWQFGNMIQCAQPGVNPLIYNNYGCFCGLGG-SGSPKDQLDRCCQVHDQCYTNAKKLPECTFLDPYVKVYSHSCSNKKASCSGSNAKCEAAVCECDRVAAHCFAQYNHVYNSAY 125          
BLAST of Phospholipase A(2) vs. Ensembl Cavefish
Match: ENSAMXT00000031293.1 (phospholipase A2-like [Source:NCBI gene;Acc:103028136])

HSP 1 Score: 46.595 bits (109), Expect = 1.931e-6
Identity = 33/115 (28.70%), Postives = 49/115 (42.61%), Query Frame = 2
Query:  122 GLIEFNEMIR-KYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSD--KTYFLAYKWEVEDNQITCKDV-GQTETELCLCDKVINICIKENWKDFNESH 454
             L +F  MI+     +NP     YG +CG G     P D +DRCCQ HD CY   K+         Y   Y     + + +C     + E  +C CD+V   C  +    +N ++
Sbjct:   23 ALWQFGNMIQCAQPGVNPLIYNNYGCFCGLGG-SGSPKDQLDRCCQVHDQCYTNAKKLPECTFLDPYVKVYSHSCSNKKASCSGSNAKCEAAVCECDRVAAHCFAQYNHVYNSAY 136          
BLAST of Phospholipase A(2) vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008199.1 (pep scaffold:Pmarinus_7.0:GL476891:241341:242643:-1 gene:ENSPMAG00000007414.1 transcript:ENSPMAT00000008199.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.5286 bits (127), Expect = 1.080e-9
Identity = 43/147 (29.25%), Postives = 65/147 (44.22%), Query Frame = 2
Query:   86 IYFICFPCLSNAG-----------LIEFNEMIRKYVKINPGKLVGYGNYC---GAGNIPVEPLDGIDRCCQKHDLCY-KLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQT-ETELCLCDKVINICIKEN-----WKDFNESHYVR 463
            ++ +CF  L   G           +  F  MI K V+INP +  GYG  C   G G    +P+D +DRCCQ H  CY +++K    S   ++  Y + +    I C   G   +   CLCDK    C   +     +++FN   Y +
Sbjct:    1 VFVLCFAVLLARGQEIEHKREKRAIWNFAWMIWKVVRINPAEYSGYGCNCLSTGHG----KPVDDVDRCCQAHTKCYEQVYKM---SCSPHWDPYYFSIRGKAIRCTRGGPPCDVHTCLCDKAAVECFARSHFNRRYRNFNRKRYCK 140          
BLAST of Phospholipase A(2) vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008463.1 (pep scaffold:Pmarinus_7.0:GL483377:12502:14271:-1 gene:ENSPMAG00000007657.1 transcript:ENSPMAT00000008463.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.9878 bits (123), Expect = 3.959e-9
Identity = 42/141 (29.79%), Postives = 64/141 (45.39%), Query Frame = 2
Query:   71 VFFMWIYFICFPCLSNAGLIEFNEMIRKYVKINPGKLVGYGNYC---GAGNIPVEPLDGIDRCCQKHDLCY-KLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQT-ETELCLCDKVINICIKEN-----WKDFNESHYVR 463
            +F   IY +     S++  + F  MI K V INP +  GYG  C   G G    +P+D +DRCCQ H  CY +++K    S   ++  Y + +    I C   G   +   C CDK    C   +     +++FN   Y +
Sbjct:    4 LFTFAIYTLIAGAFSSS--LAFALMIWKVVGINPAEYSGYGCNCLSTGHG----KPVDDVDRCCQAHTKCYEQVYKM---SCSPHWDPYYFSIRGKAIRCTRGGPPCDVHTCRCDKAAVECFARSHFNRRYRNFNRKRYCK 135          
BLAST of Phospholipase A(2) vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008209.1 (pep scaffold:Pmarinus_7.0:GL476891:251313:253691:-1 gene:ENSPMAG00000007418.1 transcript:ENSPMAT00000008209.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.8322 bits (120), Expect = 1.304e-8
Identity = 37/113 (32.74%), Postives = 52/113 (46.02%), Query Frame = 2
Query:  134 FNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCY-KLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQT-ETELCLCDKVINICIKENWKDFNESHYVRD 466
            F  MI +    NP    GYG +C  G+    P+D +DRCCQ H+ CY   +     SD     +Y++      ITC+  G   ET  C CDK    C   +  D N  ++ R+
Sbjct:   33 FAWMIYRVTGRNPLDYSGYGCHCIMGS-EGHPVDAVDRCCQVHNRCYDAAFSMGCASD---LDSYEFSTRGKAITCRKQGSPCETLACSCDKAAVECFAIHDFDDNFQNFKRN 141          
BLAST of Phospholipase A(2) vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008454.1 (pep scaffold:Pmarinus_7.0:GL483377:2289:3737:-1 gene:ENSPMAG00000007656.1 transcript:ENSPMAT00000008454.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.1358 bits (113), Expect = 6.407e-8
Identity = 37/123 (30.08%), Postives = 53/123 (43.09%), Query Frame = 2
Query:  122 GLIEFNEMIRKYVKINPGKLVGYGNYC---GAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQT-ETELCLCDKVINICIKEN-----WKDFNESHYVR 463
             +  F  MI K V INP +  GYG  C   G G    +P+D +DRCCQ H  CY+   +   S    +  Y + +    I C   G   +   C CDK    C   +     +++FN   Y +
Sbjct:    1 AIWNFAWMIWKVVGINPAEYSGYGCNCLSTGHG----KPVDDVDRCCQAHTKCYEQVNKMSCSPD--WDPYYFSIRGKAIRCTRGGPPCDVHTCRCDKAAVECFARSHFNRRYRNFNRKRYCK 117          
BLAST of Phospholipase A(2) vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008226.1 (pep scaffold:Pmarinus_7.0:GL476891:263168:265744:1 gene:ENSPMAG00000007428.1 transcript:ENSPMAT00000008226.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.9062 bits (115), Expect = 7.783e-8
Identity = 30/102 (29.41%), Postives = 45/102 (44.12%), Query Frame = 2
Query:  125 LIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQ-TETELCLCDKVINICIKE 427
            +++F  MI   +   P    GYG +CG G     P+D  DRCC+ HD CY   +        Y   Y +  +  +ITC       E ++C CD+  + C   
Sbjct:   35 VVQFGNMIECRMNRQPLDYYGYGCHCGFGGKGA-PVDPTDRCCRSHDCCYN--RAITAGCNPYSDIYHYACKHGRITCDAKNDWCERKICECDRRASACFTH 133          
BLAST of Phospholipase A(2) vs. Ensembl Nematostella
Match: EDO43504 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYD8])

HSP 1 Score: 53.5286 bits (127), Expect = 1.048e-9
Identity = 34/106 (32.08%), Postives = 45/106 (42.45%), Query Frame = 2
Query:  131 EFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCK---DVGQT---ETELCLCDKVINICIKEN 430
            +F  M++           GYGN+CG G     P+DG+DRCC  HD C   W    H  + Y   Y + V D   +C    + G     E  +C CDK    C   N
Sbjct:    1 QFGRMVKCATGRRAWDYNGYGNWCGRGGRGT-PVDGVDRCCMVHDHC---WDRLVHC-RPYSNKYSYSVSDRHPSCSITCNAGNNDSCEQSICNCDKAAAECFARN 101          
BLAST of Phospholipase A(2) vs. Ensembl Nematostella
Match: EDO28179 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T773])

HSP 1 Score: 47.3654 bits (111), Expect = 9.310e-8
Identity = 30/85 (35.29%), Postives = 41/85 (48.24%), Query Frame = 2
Query:  134 FNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDN----QITCKDVGQT 376
            F  MI+     +      YGN+CG G     P+DG+DRCC+ HDLC   W E  H D  + L Y++ +        ITC +   T
Sbjct:    2 FGRMIKCATGRSAWDYNRYGNWCGRGGSGT-PVDGVDRCCRAHDLC---WGEVNHCDPFWNL-YRYRISGKYPSCSITCSEYNHT 81          
BLAST of Phospholipase A(2) vs. Ensembl Nematostella
Match: EDO38422 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCY6])

HSP 1 Score: 48.521 bits (114), Expect = 1.046e-7
Identity = 28/87 (32.18%), Postives = 38/87 (43.68%), Query Frame = 2
Query:  125 LIEFNEMIRKYVKINP-GKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKF---HSDKTYFLAYKWEVEDNQITCKDVGQ 373
            L +   M++  +   P  K V YG YCG G   + P+D +DRCCQ HD CY      +        YF  Y+     N   C   G+
Sbjct:   40 LFQLANMMKCLIPQRPWAKYVAYGCYCGPGGEGI-PVDDVDRCCQAHDTCYDKVSSNYFCGFPGAIYFAQYRTLSSRNMPGCLTCGR 125          
BLAST of Phospholipase A(2) vs. Ensembl Nematostella
Match: EDO34714 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNJ7])

HSP 1 Score: 47.7506 bits (112), Expect = 4.988e-7
Identity = 24/55 (43.64%), Postives = 32/55 (58.18%), Query Frame = 2
Query:  122 GLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWK 286
             L +F+ MI+   K NP     YG YCG G     P+DG+D+CC  HD+CYK  +
Sbjct:   43 SLFDFHAMIKCETKRNPLDYNYYGCYCGFGGRG-SPVDGLDKCCYVHDMCYKASR 96          
BLAST of Phospholipase A(2) vs. Ensembl Nematostella
Match: EDO43503 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYD7])

HSP 1 Score: 44.2838 bits (103), Expect = 2.106e-6
Identity = 34/106 (32.08%), Postives = 49/106 (46.23%), Query Frame = 2
Query:  131 EFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCK---DVGQT---ETELCLCDKVINICIKEN 430
            +F +MI+     +      YGN+CG G     P+DG+DRCC+ HDLC   W E  H      L Y++ +     +C    + G+    E  +C CDK    C   N
Sbjct:    1 QFRKMIKCATGRSAWDYNRYGNWCGRGGGGT-PVDGVDRCCRAHDLC---WGEVKHCHPFSNL-YRYRISGIYPSCSITCNAGKNDSCEQSICNCDKAAAECFARN 101          
BLAST of Phospholipase A(2) vs. Ensembl Medaka
Match: zgc:92162 (phospholipase A2 [Source:NCBI gene;Acc:101170929])

HSP 1 Score: 48.9062 bits (115), Expect = 3.470e-7
Identity = 35/119 (29.41%), Postives = 49/119 (41.18%), Query Frame = 2
Query:  113 SNAGLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDV-GQTETELCLCDKVINICIKEN--------WKDF 442
            +  GL+E    I+     +    + YG YCG G     P D  D CC +HD CY     +     T    Y W  ED +  C D+  + E  LC CD+    C+++         W DF
Sbjct:   27 TRRGLLELAGAIKCSTGRSALAYMMYGCYCGLGG-QGWPRDRADWCCHRHDCCYG--DAELLGCHTKTAQYHWTCEDRKAECDDLKDKCEKMLCKCDREAAKCLRKAPYIQKYALWPDF 142          
BLAST of Phospholipase A(2) vs. Planmine SMEST
Match: SMESG000025979.1 (SMESG000025979.1)

HSP 1 Score: 132.109 bits (331), Expect = 6.532e-39
Identity = 64/135 (47.41%), Postives = 78/135 (57.78%), Query Frame = 2
Query:  116 NAGLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQ----ITCKDVGQTETELCLCDKVINICIKENWKDFNESHYVRDPKQRKLVTSLNELL 508
            N  L+E N M+RKY+ INPGKLVGYGNYCG GN    P+D ID CC+ HD C+ LW EK+   KTY   YKW +  +     I C    + + +LC CDK   IC+K  W  FN  HY      RK +TS N  L
Sbjct:   18 NGSLLELNNMLRKYLHINPGKLVGYGNYCGPGNNGKAPIDAIDMCCKIHDNCFGLWSEKYEDTKTYLTKYKWTLTMHGIKKFIVCGSQTRKKKDLCHCDKEFVICVKSKWHRFNHDHYSDTKNPRKFITSFNIYL 152          
BLAST of Phospholipase A(2) vs. Planmine SMEST
Match: SMESG000025902.1 (SMESG000025902.1)

HSP 1 Score: 131.339 bits (329), Expect = 1.198e-38
Identity = 64/135 (47.41%), Postives = 78/135 (57.78%), Query Frame = 2
Query:  116 NAGLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQ----ITCKDVGQTETELCLCDKVINICIKENWKDFNESHYVRDPKQRKLVTSLNELL 508
            N  L+E N M+RKY+ INPGKLVGYGNYCG GN    P+D ID CC+ HD C+ LW EK+   KTY   YKW +  +     I C    + + +LC CDK   IC+K  W  FN  HY      RK +TS N  L
Sbjct:   18 NGSLLELNNMLRKYLHINPGKLVGYGNYCGPGNNGKAPIDAIDMCCKIHDNCFGLWSEKYEDTKTYLTKYKWTLTMHGIKKFIVCGRQTRKKKDLCQCDKEFVICVKSKWHRFNHDHYSDTKNPRKFITSFNIYL 152          
BLAST of Phospholipase A(2) vs. Planmine SMEST
Match: SMESG000025979.1 (SMESG000025979.1)

HSP 1 Score: 123.25 bits (308), Expect = 9.226e-36
Identity = 60/126 (47.62%), Postives = 73/126 (57.94%), Query Frame = 2
Query:  143 MIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQ----ITCKDVGQTETELCLCDKVINICIKENWKDFNESHYVRDPKQRKLVTSLNELL 508
            M+RKY+ INPGKLVGYGNYCG GN    P+D ID CC+ HD C+ LW EK+   KTY   YKW +  +     I C    + + +LC CDK   IC+K  W  FN  HY      RK +TS N  L
Sbjct:    1 MLRKYLHINPGKLVGYGNYCGPGNNGKAPIDAIDMCCKIHDNCFGLWSEKYEDTKTYLTKYKWTLTMHGIKKFIVCGSQTRKKKDLCHCDKEFVICVKSKWHRFNHDHYSDTKNPRKFITSFNIYL 126          
BLAST of Phospholipase A(2) vs. Planmine SMEST
Match: SMESG000025902.1 (SMESG000025902.1)

HSP 1 Score: 122.479 bits (306), Expect = 1.638e-35
Identity = 60/126 (47.62%), Postives = 73/126 (57.94%), Query Frame = 2
Query:  143 MIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQ----ITCKDVGQTETELCLCDKVINICIKENWKDFNESHYVRDPKQRKLVTSLNELL 508
            M+RKY+ INPGKLVGYGNYCG GN    P+D ID CC+ HD C+ LW EK+   KTY   YKW +  +     I C    + + +LC CDK   IC+K  W  FN  HY      RK +TS N  L
Sbjct:    1 MLRKYLHINPGKLVGYGNYCGPGNNGKAPIDAIDMCCKIHDNCFGLWSEKYEDTKTYLTKYKWTLTMHGIKKFIVCGRQTRKKKDLCQCDKEFVICVKSKWHRFNHDHYSDTKNPRKFITSFNIYL 126          
BLAST of Phospholipase A(2) vs. Planmine SMEST
Match: SMESG000079712.1 (SMESG000079712.1)

HSP 1 Score: 120.168 bits (300), Expect = 2.404e-34
Identity = 57/137 (41.61%), Postives = 82/137 (59.85%), Query Frame = 2
Query:   89 YFICFPCLSNAGLIEFNEMIRKYVKINPGKLVGYGNYCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDNQITCKDVGQTETELCLCDKVINICIKENWKDFNESHYVRDPKQRKLVTSLN 499
            +F+CF  LS   LIEFN M++  +K NP  LVGYG YCG GN+P +P+D ID CC+ HD CY+ W E  H +     +Y W   +  ++C    +   +LC CD+ I  C+++N+K FN  HY R   +R  + +LN
Sbjct:   10 FFLCFT-LSLGSLIEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETW-ENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEHYRRQGIERAALGTLN 144          
The following BLAST results are available for this feature:
BLAST of Phospholipase A(2) vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PLA2G2A1.693e-1332.77phospholipase A2 group IIA [Source:HGNC Symbol;Acc... [more]
PLA2G2A1.693e-1332.77phospholipase A2 group IIA [Source:HGNC Symbol;Acc... [more]
PLA2G2A1.738e-1233.65phospholipase A2 group IIA [Source:HGNC Symbol;Acc... [more]
PLA2G51.267e-1030.53phospholipase A2 group V [Source:HGNC Symbol;Acc:H... [more]
PLA2G2D2.413e-931.86phospholipase A2 group IID [Source:HGNC Symbol;Acc... [more]
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BLAST of Phospholipase A(2) vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Y69A2AL.21.495e-1038.00Phospholipase A(2) [Source:UniProtKB/TrEMBL;Acc:Q... [more]
C07E3.91.696e-630.63Phospholipase A(2) [Source:UniProtKB/TrEMBL;Acc:Q... [more]
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BLAST of Phospholipase A(2) vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phospholipase A(2) vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:921629.367e-831.09zgc:92162 [Source:ZFIN;Acc:ZDB-GENE-040718-41][more]
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BLAST of Phospholipase A(2) vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000035968.13.201e-1031.75pep primary_assembly:Xenopus_tropicalis_v9.1:4:795... [more]
ENSXETT00000024628.12.221e-935.71pep primary_assembly:Xenopus_tropicalis_v9.1:9:319... [more]
eml63.956e-932.20echinoderm microtubule associated protein like 6 [... [more]
eml67.739e-929.55echinoderm microtubule associated protein like 6 [... [more]
eml68.172e-932.20echinoderm microtubule associated protein like 6 [... [more]
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BLAST of Phospholipase A(2) vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pla2g2d1.155e-1134.21phospholipase A2, group IID [Source:MGI Symbol;Acc... [more]
Pla2g53.483e-1130.53phospholipase A2, group V [Source:MGI Symbol;Acc:M... [more]
Pla2g53.483e-1130.53phospholipase A2, group V [Source:MGI Symbol;Acc:M... [more]
Pla2g53.483e-1130.53phospholipase A2, group V [Source:MGI Symbol;Acc:M... [more]
Pla2g53.483e-1130.53phospholipase A2, group V [Source:MGI Symbol;Acc:M... [more]
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BLAST of Phospholipase A(2) vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P0DMT3|PA2HS_ECHCO1.478e-1434.51Phospholipase A2 homolog ECO_00035 OS=Echis colora... [more]
sp|P0DMT1|PA2HS_ECHPL1.958e-1332.74Phospholipase A2 homolog EPL_00195 OS=Echis pyrami... [more]
sp|C1JAR9|PA2_SCOVI4.744e-1333.81Phospholipase A2 Scol/Pla OS=Scolopendra viridis O... [more]
sp|P14555|PA2GA_HUMAN8.130e-1332.77Phospholipase A2, membrane associated OS=Homo sapi... [more]
sp|P0DPT7|PA21_SCODE8.764e-1337.72Phospholipase A2 SSD387 OS=Scolopendra dehaani OX=... [more]
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BLAST of Phospholipase A(2) vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A218UMK13.918e-1432.56Phospholipase A(2) OS=Lonchura striata domestica O... [more]
A0A0L8IC385.507e-1432.09Phospholipase A(2) OS=Octopus bimaculoides OX=3765... [more]
A0A3M7SJW07.593e-1431.82Phospholipase A(2) OS=Brachionus plicatilis OX=101... [more]
A0A087SVA43.729e-1330.07Phospholipase A(2) (Fragment) OS=Stegodyphus mimos... [more]
A0A0A1WC824.492e-1333.07Phospholipase A2 Group IIA c OS=Echis coloratus OX... [more]
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BLAST of Phospholipase A(2) vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
zgc:921626.764e-830.25phospholipase A2-like [Source:NCBI gene;Acc:103040... [more]
ENSAMXT00000000319.21.049e-631.45phospholipase A2 [Source:NCBI gene;Acc:103024514][more]
ENSAMXT00000030049.11.381e-628.70phospholipase A2-like [Source:NCBI gene;Acc:103028... [more]
ENSAMXT00000031293.11.931e-628.70phospholipase A2-like [Source:NCBI gene;Acc:103028... [more]
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BLAST of Phospholipase A(2) vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000008199.11.080e-929.25pep scaffold:Pmarinus_7.0:GL476891:241341:242643:-... [more]
ENSPMAT00000008463.13.959e-929.79pep scaffold:Pmarinus_7.0:GL483377:12502:14271:-1 ... [more]
ENSPMAT00000008209.11.304e-832.74pep scaffold:Pmarinus_7.0:GL476891:251313:253691:-... [more]
ENSPMAT00000008454.16.407e-830.08pep scaffold:Pmarinus_7.0:GL483377:2289:3737:-1 ge... [more]
ENSPMAT00000008226.17.783e-829.41pep scaffold:Pmarinus_7.0:GL476891:263168:265744:1... [more]
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BLAST of Phospholipase A(2) vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phospholipase A(2) vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO435041.048e-932.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO281799.310e-835.29Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO384221.046e-732.18Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO347144.988e-743.64Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO435032.106e-632.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Phospholipase A(2) vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:921623.470e-729.41phospholipase A2 [Source:NCBI gene;Acc:101170929][more]
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BLAST of Phospholipase A(2) vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000025979.16.532e-3947.41SMESG000025979.1[more]
SMESG000025902.11.198e-3847.41SMESG000025902.1[more]
SMESG000025979.19.226e-3647.62SMESG000025979.1[more]
SMESG000025902.11.638e-3547.62SMESG000025902.1[more]
SMESG000079712.12.404e-3441.61SMESG000079712.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035032 ID=SMED30035032|Name=Phospholipase A(2)|organism=Schmidtea mediterranea sexual|type=transcript|length=626bp
TATATACTCATATAAATGAAATAAAATATATTTAATAAACTAGAAAATGA
CATTTTGCAAAGGAAATATTGTCTTTTTCATGTGGATTTATTTTATTTGT
TTTCCTTGCTTGTCAAACGCGGGTCTGATTGAGTTCAATGAAATGATTCG
GAAGTATGTGAAAATTAATCCCGGAAAATTGGTCGGTTATGGAAATTATT
GCGGCGCCGGAAACATCCCAGTCGAACCGTTGGACGGCATTGACAGATGT
TGTCAGAAACATGATTTGTGCTACAAACTATGGAAGGAAAAATTCCATTC
CGACAAGACCTATTTTCTGGCTTATAAATGGGAAGTCGAAGACAATCAGA
TCACTTGCAAAGATGTAGGCCAAACGGAAACCGAACTTTGCCTCTGTGAC
AAAGTAATCAATATCTGCATAAAAGAAAACTGGAAAGATTTCAATGAAAG
TCACTACGTTCGAGATCCAAAACAGAGAAAACTCGTTACATCATTGAATG
AATTACTAAGCGCTATTGAATCTTGATCTATTGCAATCTTAAGAATTTTT
ATTGTTAAAATAATGAATGGAATGCAATTTATTGTCTATGAATAAAAATA
ATAAAAACAATCAAATATTATTTATG
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protein sequence of SMED30035032-orf-1

>SMED30035032-orf-1 ID=SMED30035032-orf-1|Name=SMED30035032-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=160bp
MTFCKGNIVFFMWIYFICFPCLSNAGLIEFNEMIRKYVKINPGKLVGYGN
YCGAGNIPVEPLDGIDRCCQKHDLCYKLWKEKFHSDKTYFLAYKWEVEDN
QITCKDVGQTETELCLCDKVINICIKENWKDFNESHYVRDPKQRKLVTSL
NELLSAIES*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0005543phospholipid binding
GO:0005509calcium ion binding
GO:0004623phospholipase A2 activity
GO:0016787hydrolase activity
Vocabulary: cellular component
TermDefinition
GO:0005615extracellular space
GO:0005783endoplasmic reticulum
GO:0005576extracellular region
Vocabulary: biological process
TermDefinition
GO:0046473phosphatidic acid metabolic process
GO:0070374positive regulation of ERK1 and ERK2 cascade
GO:0016042lipid catabolic process
GO:0006629lipid metabolic process
GO:0006644phospholipid metabolic process
GO:0050482arachidonic acid secretion
Vocabulary: INTERPRO
TermDefinition
IPR036444PLipase_A2_dom_sf
IPR001211PLipase_A2
IPR016090PLipase_A2_dom
IPR033113PLipase_A2_His_AS
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001211Phospholipase A2PRINTSPR00389PHPHLIPASEA2coord: 110..126
score: 31.53
coord: 61..79
score: 54.15
coord: 27..37
score: 39.24
coord: 92..106
score: 27.34
IPR001211Phospholipase A2PANTHERPTHR11716PHOSPHOLIPASE A2 FAMILY MEMBERcoord: 24..140
IPR016090Phospholipase A2 domainSMARTSM00085PA-fincoord: 26..146
e-value: 2.7E-16
score: 70.1
IPR016090Phospholipase A2 domainPFAMPF00068Phospholip_A2_1coord: 27..136
e-value: 2.2E-24
score: 86.1
IPR016090Phospholipase A2 domainCDDcd00125PLA2ccoord: 26..142
e-value: 1.89919E-22
score: 84.2236
IPR036444Phospholipase A2 domain superfamilyGENE3DG3DSA:1.20.90.10coord: 26..148
e-value: 8.4E-27
score: 95.7
IPR036444Phospholipase A2 domain superfamilySUPERFAMILYSSF48619Phospholipase A2, PLA2coord: 27..141
NoneNo IPR availablePANTHERPTHR11716:SF84PHOSPHOLIPASE A2coord: 24..140
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..25
score: 0.81
NoneNo IPR availableTMHMMTMhelixcoord: 7..29
IPR033113Phospholipase A2, histidine active sitePROSITEPS00118PA2_HIScoord: 68..75