SMED30035024

Overview
NameSMED30035024
Smed IDSMED30035024
Length (bp)386
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30035024 (SMED30035024) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30035024 (SMED30035024) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30035024 (SMED30035024) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30035024 (SMED30035024) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035024

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30035024SMESG000074987.1 SmedASXL_079065SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30035024SMESG000074987.1 dd_Smed_v4_3607_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30035024 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001926:51628..52052 +1869
v31.019296:1331..1755 +1851
Homology
[Close]
BLAST of SMED30035024 vs. Ensembl Human
Match: MRPS33 (mitochondrial ribosomal protein S33 [Source:HGNC Symbol;Acc:HGNC:16634])

HSP 1 Score: 62.003 bits (149), Expect = 5.153e-13
Identity = 34/85 (40.00%), Postives = 53/85 (62.35%), Query Frame = -3 
Query:  133 SNYGKKIAQLSSQIFMEINNPS-PQLRKVLKLFKNKP--RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELR 378
            S Y  ++++LS+++F E+  P+  +  KV+KLF   P  ++    DWY        + + LR LGLYRDEHQDF +E +RL++LR
Sbjct:    5 SEYAFRMSRLSARLFGEVTRPTNSKSMKVVKLFSELPLAKKKETYDWYPNHHTYAELMQTLRFLGLYRDEHQDFMDEQKRLKKLR 89
[Close]
BLAST of SMED30035024 vs. Ensembl Human
Match: MRPS33 (mitochondrial ribosomal protein S33 [Source:HGNC Symbol;Acc:HGNC:16634])

HSP 1 Score: 62.003 bits (149), Expect = 5.153e-13
Identity = 34/85 (40.00%), Postives = 53/85 (62.35%), Query Frame = -3 
Query:  133 SNYGKKIAQLSSQIFMEINNPS-PQLRKVLKLFKNKP--RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELR 378
            S Y  ++++LS+++F E+  P+  +  KV+KLF   P  ++    DWY        + + LR LGLYRDEHQDF +E +RL++LR
Sbjct:    5 SEYAFRMSRLSARLFGEVTRPTNSKSMKVVKLFSELPLAKKKETYDWYPNHHTYAELMQTLRFLGLYRDEHQDFMDEQKRLKKLR 89
[Close]
BLAST of SMED30035024 vs. Ensembl Human
Match: MRPS33 (mitochondrial ribosomal protein S33 [Source:HGNC Symbol;Acc:HGNC:16634])

HSP 1 Score: 62.003 bits (149), Expect = 5.153e-13
Identity = 34/85 (40.00%), Postives = 53/85 (62.35%), Query Frame = -3 
Query:  133 SNYGKKIAQLSSQIFMEINNPS-PQLRKVLKLFKNKP--RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELR 378
            S Y  ++++LS+++F E+  P+  +  KV+KLF   P  ++    DWY        + + LR LGLYRDEHQDF +E +RL++LR
Sbjct:    5 SEYAFRMSRLSARLFGEVTRPTNSKSMKVVKLFSELPLAKKKETYDWYPNHHTYAELMQTLRFLGLYRDEHQDFMDEQKRLKKLR 89
[Close]
BLAST of SMED30035024 vs. Ensembl Human
Match: MRPS33 (mitochondrial ribosomal protein S33 [Source:HGNC Symbol;Acc:HGNC:16634])

HSP 1 Score: 62.003 bits (149), Expect = 5.153e-13
Identity = 34/85 (40.00%), Postives = 53/85 (62.35%), Query Frame = -3 
Query:  133 SNYGKKIAQLSSQIFMEINNPS-PQLRKVLKLFKNKP--RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELR 378
            S Y  ++++LS+++F E+  P+  +  KV+KLF   P  ++    DWY        + + LR LGLYRDEHQDF +E +RL++LR
Sbjct:    5 SEYAFRMSRLSARLFGEVTRPTNSKSMKVVKLFSELPLAKKKETYDWYPNHHTYAELMQTLRFLGLYRDEHQDFMDEQKRLKKLR 89
[Close]
BLAST of SMED30035024 vs. Ensembl Human
Match: MRPS33 (mitochondrial ribosomal protein S33 [Source:HGNC Symbol;Acc:HGNC:16634])

HSP 1 Score: 58.151 bits (139), Expect = 1.426e-11
Identity = 32/79 (40.51%), Postives = 50/79 (63.29%), Query Frame = -3 
Query:  133 IAQLSSQIFMEINNPS-PQLRKVLKLFKNKP--RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELR 360
            +++LS+++F E+  P+  +  KV+KLF   P  ++    DWY        + + LR LGLYRDEHQDF +E +RL++LR
Sbjct:    1 MSRLSARLFGEVTRPTNSKSMKVVKLFSELPLAKKKETYDWYPNHHTYAELMQTLRFLGLYRDEHQDFMDEQKRLKKLR 79
[Close]
BLAST of SMED30035024 vs. Ensembl Fly
Match: mRpS33 (gene:FBgn0038426 transcript:FBtr0083316)

HSP 1 Score: 73.559 bits (179), Expect = 1.086e-17
Identity = 39/95 (41.05%), Postives = 64/95 (67.37%), Query Frame = -3 
Query:  109 MASNYGKKIAQLSSQIFMEINNPSPQLR-KVLKLFKNKP--RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRK 384
            + + Y +++  LS++IF E+   + +   KV+++F  +P  ++   ++WY    E   + ++LRD GL+RDEHQDFKEEM+RLR+LRGK A P+K
Sbjct:   11 VGTQYARRMNYLSNRIFGEVARTTNEKSMKVVRMFSEEPIHKRDYVINWYPRHVETHLLMKNLRDYGLFRDEHQDFKEEMKRLRKLRGK-APPKK 104
[Close]
BLAST of SMED30035024 vs. Ensembl Zebrafish
Match: mrps33 (mitochondrial ribosomal protein S33 [Source:ZFIN;Acc:ZDB-GENE-040715-6])

HSP 1 Score: 77.7962 bits (190), Expect = 2.263e-19
Identity = 45/94 (47.87%), Postives = 66/94 (70.21%), Query Frame = -3 
Query:  109 SNYGKKIAQLSSQIFMEINNPS-PQLRKVLKLFKNKP---RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRK 378
            SNY  ++A+LS++IF +++  +  +  KV++LFK  P   R+ +  DWY P     S+T+ LR +GL+RDEHQDFKEEM+RLR+LRGK  +P K
Sbjct:    5 SNYALRMARLSARIFGDVSRQTDARSLKVVELFKEPPLAQRKEV-YDWYPPHKIYYSMTQRLRYMGLFRDEHQDFKEEMRRLRKLRGK-GRPNK 96
[Close]
BLAST of SMED30035024 vs. Ensembl Zebrafish
Match: mrps33 (mitochondrial ribosomal protein S33 [Source:ZFIN;Acc:ZDB-GENE-040715-6])

HSP 1 Score: 77.7962 bits (190), Expect = 2.263e-19
Identity = 45/94 (47.87%), Postives = 66/94 (70.21%), Query Frame = -3 
Query:  109 SNYGKKIAQLSSQIFMEINNPS-PQLRKVLKLFKNKP---RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRK 378
            SNY  ++A+LS++IF +++  +  +  KV++LFK  P   R+ +  DWY P     S+T+ LR +GL+RDEHQDFKEEM+RLR+LRGK  +P K
Sbjct:    5 SNYALRMARLSARIFGDVSRQTDARSLKVVELFKEPPLAQRKEV-YDWYPPHKIYYSMTQRLRYMGLFRDEHQDFKEEMRRLRKLRGK-GRPNK 96
[Close]
BLAST of SMED30035024 vs. Ensembl Xenopus
Match: MRPS33 (mitochondrial ribosomal protein S33 [Source:NCBI gene;Acc:100379725])

HSP 1 Score: 83.9593 bits (206), Expect = 1.312e-21
Identity = 46/94 (48.94%), Postives = 66/94 (70.21%), Query Frame = -3 
Query:  109 SNYGKKIAQLSSQIFMEINNPSPQLR-KVLKLFKNKP---RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRK 378
            S+Y  ++++LS++IF E+  P+ Q   KV+K+F  +P   R+ +  DWY P     S+ R+LR LGLYRDEH+DFKEEM+RLR+LRGK   P+K
Sbjct:    5 SSYALRMSRLSARIFGEVVRPTDQRSMKVVKMFSEEPLAKRKEV-FDWYPPHNTYVSLMRNLRYLGLYRDEHEDFKEEMRRLRKLRGK-GTPKK 96
[Close]
BLAST of SMED30035024 vs. Ensembl Mouse
Match: Mrps33 (mitochondrial ribosomal protein S33 [Source:MGI Symbol;Acc:MGI:1338046])

HSP 1 Score: 68.9366 bits (167), Expect = 7.120e-16
Identity = 40/93 (43.01%), Postives = 60/93 (64.52%), Query Frame = -3 
Query:  109 SNYGKKIAQLSSQIFMEINNPS-PQLRKVLKLFKNKP--RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRK 378
            S Y  ++++LS++IF E+   +  +  KV+ LF  +P  ++    DWY       ++  +LR LGLYRDEHQDFK+E +RL++LRGK  KPRK
Sbjct:    5 SEYALRMSRLSARIFGEVARSTDSKSMKVVSLFSEQPLAKKKETYDWYPNHNTYFALMGNLRFLGLYRDEHQDFKDEQRRLKKLRGK-GKPRK 96
[Close]
BLAST of SMED30035024 vs. UniProt/SwissProt
Match: sp|P82926|RT33_BOVIN (28S ribosomal protein S33, mitochondrial OS=Bos taurus OX=9913 GN=MRPS33 PE=1 SV=2)

HSP 1 Score: 70.0922 bits (170), Expect = 1.567e-15
Identity = 40/93 (43.01%), Postives = 60/93 (64.52%), Query Frame = -3 
Query:  109 SNYGKKIAQLSSQIFMEINNPS-PQLRKVLKLFKNKP--RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRK 378
            S Y  ++++LS+++F E+  P+  +  KV+KLF  +P  ++    DWY       ++   LR +GLYRDEHQDFK+E  RL++LRGK  KPRK
Sbjct:    5 SEYALRMSRLSARLFSEVARPTDSKSMKVVKLFSEQPLAKRKETYDWYPNHNTYFALMGILRSVGLYRDEHQDFKDEQLRLKKLRGK-VKPRK 96
[Close]
BLAST of SMED30035024 vs. UniProt/SwissProt
Match: sp|Q9D2R8|RT33_MOUSE (28S ribosomal protein S33, mitochondrial OS=Mus musculus OX=10090 GN=Mrps33 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 4.981e-15
Identity = 40/93 (43.01%), Postives = 60/93 (64.52%), Query Frame = -3 
Query:  109 SNYGKKIAQLSSQIFMEINNPS-PQLRKVLKLFKNKP--RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRK 378
            S Y  ++++LS++IF E+   +  +  KV+ LF  +P  ++    DWY       ++  +LR LGLYRDEHQDFK+E +RL++LRGK  KPRK
Sbjct:    5 SEYALRMSRLSARIFGEVARSTDSKSMKVVSLFSEQPLAKKKETYDWYPNHNTYFALMGNLRFLGLYRDEHQDFKDEQRRLKKLRGK-GKPRK 96
[Close]
BLAST of SMED30035024 vs. UniProt/SwissProt
Match: sp|Q9Y291|RT33_HUMAN (28S ribosomal protein S33, mitochondrial OS=Homo sapiens OX=9606 GN=MRPS33 PE=1 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.475e-12
Identity = 34/85 (40.00%), Postives = 53/85 (62.35%), Query Frame = -3 
Query:  133 SNYGKKIAQLSSQIFMEINNPS-PQLRKVLKLFKNKP--RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELR 378
            S Y  ++++LS+++F E+  P+  +  KV+KLF   P  ++    DWY        + + LR LGLYRDEHQDF +E +RL++LR
Sbjct:    5 SEYAFRMSRLSARLFGEVTRPTNSKSMKVVKLFSELPLAKKKETYDWYPNHHTYAELMQTLRFLGLYRDEHQDFMDEQKRLKKLR 89
[Close]
BLAST of SMED30035024 vs. TrEMBL
Match: T1F9A6 (Uncharacterized protein OS=Helobdella robusta OX=6412 GN=20205405 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 2.246e-20
Identity = 50/93 (53.76%), Postives = 67/93 (72.04%), Query Frame = -3 
Query:  109 SNYGKKIAQLSSQIFMEINNPSPQ-LRKVLKLFKNKP--RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRK 378
            SNY K+IA+LS++IF E+  P+P  + KV+K+F  KP  +Q    ++Y    ELTS+ + LR LGLYRDEH DFK+EM+RLR LRGK  KP+K
Sbjct:    6 SNYAKQIARLSARIFGEVVRPTPSPMLKVVKIFSAKPLDKQPEFYNYYPRHVELTSLMKRLRFLGLYRDEHLDFKDEMKRLRALRGK-VKPKK 97
[Close]
BLAST of SMED30035024 vs. TrEMBL
Match: A0A0L8FIP5 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22018476mg PE=4 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.946e-19
Identity = 46/96 (47.92%), Postives = 70/96 (72.92%), Query Frame = -3 
Query:  109 MASNYGKKIAQLSSQIFMEINNPSPQ-LRKVLKLFKNKPRQ---HIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRK 384
            MASNY +++A+LS++IF E+  P P+   ++ +LFK++P     H+ +D+Y P+ E+ S+   LR+ GL+RDEH DFK+EM+RLR LRGK  KP+K
Sbjct:    1 MASNYAQRMARLSARIFGEVVRPIPKKTNRLFRLFKDRPYNMDPHV-VDYYPPLREINSLMLRLRNHGLFRDEHLDFKDEMKRLRVLRGK-TKPKK 94
[Close]
BLAST of SMED30035024 vs. TrEMBL
Match: A0A1B8XY61 (Mitochondrial ribosomal protein S33 OS=Xenopus tropicalis OX=8364 GN=mrps33 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 2.060e-18
Identity = 46/94 (48.94%), Postives = 66/94 (70.21%), Query Frame = -3 
Query:  109 SNYGKKIAQLSSQIFMEINNPSPQLR-KVLKLFKNKP---RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRK 378
            S+Y  ++++LS++IF E+  P+ Q   KV+K+F  +P   R+ +  DWY P     S+ R+LR LGLYRDEH+DFKEEM+RLR+LRGK   P+K
Sbjct:    5 SSYALRMSRLSARIFGEVVRPTDQRSMKVVKMFSEEPLAKRKEV-FDWYPPHNTYVSLMRNLRYLGLYRDEHEDFKEEMRRLRKLRGK-GTPKK 96
[Close]
BLAST of SMED30035024 vs. TrEMBL
Match: A0A1U7RSM9 (28S ribosomal protein S33, mitochondrial OS=Alligator sinensis OX=38654 GN=MRPS33 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 5.536e-18
Identity = 46/93 (49.46%), Postives = 63/93 (67.74%), Query Frame = -3 
Query:  109 SNYGKKIAQLSSQIFMEINNP-SPQLRKVLKLFKNKP--RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRK 378
            SNY  ++A+LS++IF E+ +P S +  KV+KLF  +P  ++    DWY P    + + R +R LGLYRDEH DF+EEM+RLR LRGK  KP K
Sbjct:    5 SNYALQMARLSARIFGEVVSPTSNKSMKVVKLFSEQPVGKKKEVFDWYPPHHSYSGLMRQIRFLGLYRDEHADFQEEMKRLRRLRGK-GKPMK 96
[Close]
BLAST of SMED30035024 vs. TrEMBL
Match: A0A1L8GQH2 (Uncharacterized protein OS=Xenopus laevis OX=8355 GN=XELAEV_18019799mg PE=4 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 8.457e-18
Identity = 46/94 (48.94%), Postives = 65/94 (69.15%), Query Frame = -3 
Query:  109 SNYGKKIAQLSSQIFMEINNPSPQLR-KVLKLFKNKP---RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRK 378
            S+Y  ++++LS++IF E+  P+ Q   KV+K+F   P   R+ +  DWY P     S+ R+LR LGLYRDEH+DFKEEM+RLR+LRGK   P+K
Sbjct:    5 SSYALRMSRLSARIFGEVVRPTDQRSMKVVKMFSEPPLAKRKEV-YDWYPPHNTYVSLMRNLRYLGLYRDEHEDFKEEMRRLRKLRGK-GTPKK 96
[Close]
BLAST of SMED30035024 vs. Ensembl Cavefish
Match: mrps33 (mitochondrial ribosomal protein S33 [Source:NCBI gene;Acc:103036627])

HSP 1 Score: 67.0106 bits (162), Expect = 2.793e-15
Identity = 38/83 (45.78%), Postives = 55/83 (66.27%), Query Frame = -3 
Query:  142 SNYGKKIAQLSSQIFMEI-NNPSPQLRKVLKLFKNKP---RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLR 378
            SNY  ++A+LS++IF ++      +  KV++LFK  P   R+ +  DWY       S+T+ LR +GL+RDEHQDFKEEM+RLR
Sbjct:    5 SNYAMRMARLSARIFGDVVRTTDSKSMKVVQLFKEPPMAQRKEV-YDWYPQHKIYYSLTQKLRYMGLFRDEHQDFKEEMRRLR 86
[Close]
BLAST of SMED30035024 vs. Ensembl Sea Lamprey
Match: mrps33 (mitochondrial ribosomal protein S33 [Source:ZFIN;Acc:ZDB-GENE-040715-6])

HSP 1 Score: 63.1586 bits (152), Expect = 1.957e-14
Identity = 34/81 (41.98%), Postives = 52/81 (64.20%), Query Frame = -3 
Query:  145 SNYGKKIAQLSSQIFMEINNPS-PQLRKVLKLFKNKP--RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRL 378
            SNY + +A+LS++IF E+  P+     KV+ L   +P  +Q   +++Y    E + + R LR  GLYRDEH+DF+EEM+RL
Sbjct:    8 SNYARHMARLSARIFGEVARPTDASSMKVVALMSERPMAKQPDVVNYYPAHQEYSQLMRTLRFHGLYRDEHEDFREEMKRL 88
[Close]
BLAST of SMED30035024 vs. Ensembl Nematostella
Match: EDO34082 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQA5])

HSP 1 Score: 65.4698 bits (158), Expect = 4.160e-15
Identity = 35/87 (40.23%), Postives = 52/87 (59.77%), Query Frame = -3 
Query:  127 MASNYGKKIAQLSSQIFMEINNP-SPQLRKVLKLFKNKPRQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGK 384
            M++NY +K+A L ++IF ++  P + Q  KV+  F  +P       +Y P+ +  S+   LR LG YRD+H DFKEEM   R+ RGK
Sbjct:    1 MSTNYARKMALLQARIFGKLTRPVTKQSYKVVTHFSRRPLGGAINSYYPPLKQFNSLLLRLRHLGFYRDDHLDFKEEMAVRRKERGK 87
[Close]
BLAST of SMED30035024 vs. Ensembl Medaka
Match: mrps33 (mitochondrial ribosomal protein S33 [Source:NCBI gene;Acc:111946956])

HSP 1 Score: 75.485 bits (184), Expect = 1.371e-18
Identity = 43/94 (45.74%), Postives = 65/94 (69.15%), Query Frame = -3 
Query:  109 SNYGKKIAQLSSQIFMEINNPSPQLR-KVLKLFKNKP---RQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRK 378
            SNY  ++A+LSS+IF E+ +P+     KV+++F+  P   R+ +  DWY       ++T+ LR +GL+RDEH+DFKEEM+RLR+LRGK   P+K
Sbjct:    5 SNYALRMARLSSRIFGEVAHPTDSRSLKVVQMFQEPPLAKRKEV-YDWYPQHKIYYAMTQKLRYMGLFRDEHEDFKEEMRRLRKLRGK-GTPKK 96
[Close]
BLAST of SMED30035024 vs. Planmine SMEST
Match: SMESG000074987.1 (SMESG000074987.1)

HSP 1 Score: 206.838 bits (525), Expect = 1.409e-70
Identity = 99/99 (100.00%), Postives = 99/99 (100.00%), Query Frame = -3 
Query:   88 MASNYGKKIAQLSSQIFMEINNPSPQLRKVLKLFKNKPRQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRKFNQSQVK 384
            MASNYGKKIAQLSSQIFMEINNPSPQLRKVLKLFKNKPRQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRKFNQSQVK
Sbjct:    1 MASNYGKKIAQLSSQIFMEINNPSPQLRKVLKLFKNKPRQHIQLDWYSPVCELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRKFNQSQVK 99
The following BLAST results are available for this feature:
BLAST of SMED30035024 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MRPS335.153e-1340.00mitochondrial ribosomal protein S33 [Source:HGNC S... [more]
MRPS335.153e-1340.00mitochondrial ribosomal protein S33 [Source:HGNC S... [more]
MRPS335.153e-1340.00mitochondrial ribosomal protein S33 [Source:HGNC S... [more]
MRPS335.153e-1340.00mitochondrial ribosomal protein S33 [Source:HGNC S... [more]
MRPS331.426e-1140.51mitochondrial ribosomal protein S33 [Source:HGNC S... [more]
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BLAST of SMED30035024 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035024 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mRpS331.086e-1741.05gene:FBgn0038426 transcript:FBtr0083316[more]
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BLAST of SMED30035024 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mrps332.263e-1947.87mitochondrial ribosomal protein S33 [Source:ZFIN;A... [more]
mrps332.263e-1947.87mitochondrial ribosomal protein S33 [Source:ZFIN;A... [more]
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BLAST of SMED30035024 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MRPS331.312e-2148.94mitochondrial ribosomal protein S33 [Source:NCBI g... [more]
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BLAST of SMED30035024 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Mrps337.120e-1643.01mitochondrial ribosomal protein S33 [Source:MGI Sy... [more]
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BLAST of SMED30035024 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|P82926|RT33_BOVIN1.567e-1543.0128S ribosomal protein S33, mitochondrial OS=Bos ta... [more]
sp|Q9D2R8|RT33_MOUSE4.981e-1543.0128S ribosomal protein S33, mitochondrial OS=Mus mu... [more]
sp|Q9Y291|RT33_HUMAN2.475e-1240.0028S ribosomal protein S33, mitochondrial OS=Homo s... [more]
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BLAST of SMED30035024 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1F9A62.246e-2053.76Uncharacterized protein OS=Helobdella robusta OX=6... [more]
A0A0L8FIP51.946e-1947.92Uncharacterized protein OS=Octopus bimaculoides OX... [more]
A0A1B8XY612.060e-1848.94Mitochondrial ribosomal protein S33 OS=Xenopus tro... [more]
A0A1U7RSM95.536e-1849.4628S ribosomal protein S33, mitochondrial OS=Alliga... [more]
A0A1L8GQH28.457e-1848.94Uncharacterized protein OS=Xenopus laevis OX=8355 ... [more]
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BLAST of SMED30035024 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrps332.793e-1545.78mitochondrial ribosomal protein S33 [Source:NCBI g... [more]
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BLAST of SMED30035024 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrps331.957e-1441.98mitochondrial ribosomal protein S33 [Source:ZFIN;A... [more]
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BLAST of SMED30035024 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035024 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO340824.160e-1540.23Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30035024 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrps331.371e-1845.74mitochondrial ribosomal protein S33 [Source:NCBI g... [more]
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BLAST of SMED30035024 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000074987.11.409e-70100.00SMESG000074987.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035024 ID=SMED30035024|Name=SMED30035024|organism=Schmidtea mediterranea sexual|type=transcript|length=386bp
TTTTAAAAGGCTAAATTTTTTATTTAAGACAATCCTTTATTAAATAAAAA
CTAAATAATTTAATTGCACTGAAGTTTCCTATTATTACTTTACTTGCGAT
TGGTTAAATTTACGTGGTTTAGCAAATTTACCACGTAATTCTCTCAAGCG
TTGCATTTCTTCTTTGAAATCTTGATGTTCATCTCGATAAAGACCTAAAT
CTCGAAGATGTCTAGTAATACTGGTAAGCTCGCAAACTGGAGAATACCAA
TCAAGTTGAATATGTTGACGTGGCTTATTTTTAAATAATTTCAAAACTTT
TCGCAATTGCGGAGAGGGATTATTTATTTCCATAAAAATTTGACTAGATA
ATTGGGCAATTTTCTTACCGTAATTCGAAGCCATAA
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protein sequence of SMED30035024-orf-1

>SMED30035024-orf-1 ID=SMED30035024-orf-1|Name=SMED30035024-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp
MASNYGKKIAQLSSQIFMEINNPSPQLRKVLKLFKNKPRQHIQLDWYSPV
CELTSITRHLRDLGLYRDEHQDFKEEMQRLRELRGKFAKPRKFNQSQVK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR013219Ribosomal_S27/S33_mit