SMED30035012

Overview
NameSMED30035012
Smed IDSMED30035012
Length (bp)2495
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30035012 (SMED30035012) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30035012 (SMED30035012) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30035012 (SMED30035012) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30035012 (SMED30035012) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30035012

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30035012h1SMcG0009270 dd_Smed_v4_16634_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30035012h1SMcG0009270 dd_Smed_v4_16634_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30035012 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000180:139366..141860 +12475
Homology
[Close]
BLAST of SMED30035012 vs. Planmine SMEST
Match: SMESG000049841.1 (SMESG000049841.1)

HSP 1 Score: 1312.36 bits (3395), Expect = 0.000e+0
Identity = 748/748 (100.00%), Postives = 748/748 (100.00%), Query Frame = 1 
Query:  247 MDRLTKCLMNGFQNDKNGGIFNYISKVINGSSYERLSESSTWVNYVKSIFVSKSSNKTYLTLAXXXXXXXXXXXXXGVVLMRRCLRKLKANPSDLRLSKNFSRFIYFILGFLTISFILKVAXXXXXXXXXXXXYRSGNQSLLQFQSFINHSLIDFQKKINLXXXXXXXXXXXXXXXFAIMSRLMVDLQVKTLFDEMPDFIDNLMLVNNSFHRISLYSKQHHFQVLKSIGXXXXXXXXXXXXXXXXXRHKNCAEIDNLLQIITNNKPNTNLKFDSSIFITKVHELVPMEVDILKIKREFDEINRDMKKVAPEALADFKRDIPLDLIEVKKNQIFIMMSNSAVKIQKIKEKSSKNSKYIDTHLRSLEVFAIIIYTLINFFEYLIILKFTFNEKMSKTIVFFNLSNILMILFITVASVLLVFSFLILTAIYNESFRYFENENENLSIIDRILSTNINNIYIDIKTKIIENLGEYRAFIILPITLPNQVLGTIKRNCTHSKSIFTILKFDSIVDFEALKKSDFFQKLILKSKMSSDTKIINLLKKISIPDIKKEYENIIYDLLRKILNKNDNEINLKSVLGILQYINNTKQLMREIRASKCPKLTAIPTQNLFSGVDNVIFTNYKILQTLFKDFQAIERNLFLPEKFKKLFPDIYQSQLNIKNKSLIIVNFHRIFDQEIRSTFDKLFSSLKDFYQIKILKNLADCKLFEDFVKDLSSFSVVTISINTILVGAXXXXXXXXXXXXXXYANFYK 2490
            MDRLTKCLMNGFQNDKNGGIFNYISKVINGSSYERLSESSTWVNYVKSIFVSKSSNKTYLTLAILLSLFFIFLSIIGVVLMRRCLRKLKANPSDLRLSKNFSRFIYFILGFLTISFILKVAFFSSTFSTNSTFYRSGNQSLLQFQSFINHSLIDFQKKINLNSLIENSTTIINSTIFAIMSRLMVDLQVKTLFDEMPDFIDNLMLVNNSFHRISLYSKQHHFQVLKSIGKLESISDLLSDKSLNLSRHKNCAEIDNLLQIITNNKPNTNLKFDSSIFITKVHELVPMEVDILKIKREFDEINRDMKKVAPEALADFKRDIPLDLIEVKKNQIFIMMSNSAVKIQKIKEKSSKNSKYIDTHLRSLEVFAIIIYTLINFFEYLIILKFTFNEKMSKTIVFFNLSNILMILFITVASVLLVFSFLILTAIYNESFRYFENENENLSIIDRILSTNINNIYIDIKTKIIENLGEYRAFIILPITLPNQVLGTIKRNCTHSKSIFTILKFDSIVDFEALKKSDFFQKLILKSKMSSDTKIINLLKKISIPDIKKEYENIIYDLLRKILNKNDNEINLKSVLGILQYINNTKQLMREIRASKCPKLTAIPTQNLFSGVDNVIFTNYKILQTLFKDFQAIERNLFLPEKFKKLFPDIYQSQLNIKNKSLIIVNFHRIFDQEIRSTFDKLFSSLKDFYQIKILKNLADCKLFEDFVKDLSSFSVVTISINTILVGALLEILFFFIFIIIIYANFYK
Sbjct:    1 MDRLTKCLMNGFQNDKNGGIFNYISKVINGSSYERLSESSTWVNYVKSIFVSKSSNKTYLTLAILLSLFFIFLSIIGVVLMRRCLRKLKANPSDLRLSKNFSRFIYFILGFLTISFILKVAFFSSTFSTNSTFYRSGNQSLLQFQSFINHSLIDFQKKINLNSLIENSTTIINSTIFAIMSRLMVDLQVKTLFDEMPDFIDNLMLVNNSFHRISLYSKQHHFQVLKSIGKLESISDLLSDKSLNLSRHKNCAEIDNLLQIITNNKPNTNLKFDSSIFITKVHELVPMEVDILKIKREFDEINRDMKKVAPEALADFKRDIPLDLIEVKKNQIFIMMSNSAVKIQKIKEKSSKNSKYIDTHLRSLEVFAIIIYTLINFFEYLIILKFTFNEKMSKTIVFFNLSNILMILFITVASVLLVFSFLILTAIYNESFRYFENENENLSIIDRILSTNINNIYIDIKTKIIENLGEYRAFIILPITLPNQVLGTIKRNCTHSKSIFTILKFDSIVDFEALKKSDFFQKLILKSKMSSDTKIINLLKKISIPDIKKEYENIIYDLLRKILNKNDNEINLKSVLGILQYINNTKQLMREIRASKCPKLTAIPTQNLFSGVDNVIFTNYKILQTLFKDFQAIERNLFLPEKFKKLFPDIYQSQLNIKNKSLIIVNFHRIFDQEIRSTFDKLFSSLKDFYQIKILKNLADCKLFEDFVKDLSSFSVVTISINTILVGALLEILFFFIFIIIIYANFYK 748
[Close]
BLAST of SMED30035012 vs. Planmine SMEST
Match: SMESG000049841.1 (SMESG000049841.1)

HSP 1 Score: 1292.72 bits (3344), Expect = 0.000e+0
Identity = 740/740 (100.00%), Postives = 740/740 (100.00%), Query Frame = 1 
Query:  271 MNGFQNDKNGGIFNYISKVINGSSYERLSESSTWVNYVKSIFVSKSSNKTYLTLAXXXXXXXXXXXXXGVVLMRRCLRKLKANPSDLRLSKNFSRFIYFILGFLTISFILKVAXXXXXXXXXXXXYRSGNQSLLQFQSFINHSLIDFQKKINLXXXXXXXXXXXXXXXFAIMSRLMVDLQVKTLFDEMPDFIDNLMLVNNSFHRISLYSKQHHFQVLKSIGXXXXXXXXXXXXXXXXXRHKNCAEIDNLLQIITNNKPNTNLKFDSSIFITKVHELVPMEVDILKIKREFDEINRDMKKVAPEALADFKRDIPLDLIEVKKNQIFIMMSNSAVKIQKIKEKSSKNSKYIDTHLRSLEVFAIIIYTLINFFEYLIILKFTFNEKMSKTIVFFNLSNILMILFITVASVLLVFSFLILTAIYNESFRYFENENENLSIIDRILSTNINNIYIDIKTKIIENLGEYRAFIILPITLPNQVLGTIKRNCTHSKSIFTILKFDSIVDFEALKKSDFFQKLILKSKMSSDTKIINLLKKISIPDIKKEYENIIYDLLRKILNKNDNEINLKSVLGILQYINNTKQLMREIRASKCPKLTAIPTQNLFSGVDNVIFTNYKILQTLFKDFQAIERNLFLPEKFKKLFPDIYQSQLNIKNKSLIIVNFHRIFDQEIRSTFDKLFSSLKDFYQIKILKNLADCKLFEDFVKDLSSFSVVTISINTILVGAXXXXXXXXXXXXXXYANFYK 2490
            MNGFQNDKNGGIFNYISKVINGSSYERLSESSTWVNYVKSIFVSKSSNKTYLTLAILLSLFFIFLSIIGVVLMRRCLRKLKANPSDLRLSKNFSRFIYFILGFLTISFILKVAFFSSTFSTNSTFYRSGNQSLLQFQSFINHSLIDFQKKINLNSLIENSTTIINSTIFAIMSRLMVDLQVKTLFDEMPDFIDNLMLVNNSFHRISLYSKQHHFQVLKSIGKLESISDLLSDKSLNLSRHKNCAEIDNLLQIITNNKPNTNLKFDSSIFITKVHELVPMEVDILKIKREFDEINRDMKKVAPEALADFKRDIPLDLIEVKKNQIFIMMSNSAVKIQKIKEKSSKNSKYIDTHLRSLEVFAIIIYTLINFFEYLIILKFTFNEKMSKTIVFFNLSNILMILFITVASVLLVFSFLILTAIYNESFRYFENENENLSIIDRILSTNINNIYIDIKTKIIENLGEYRAFIILPITLPNQVLGTIKRNCTHSKSIFTILKFDSIVDFEALKKSDFFQKLILKSKMSSDTKIINLLKKISIPDIKKEYENIIYDLLRKILNKNDNEINLKSVLGILQYINNTKQLMREIRASKCPKLTAIPTQNLFSGVDNVIFTNYKILQTLFKDFQAIERNLFLPEKFKKLFPDIYQSQLNIKNKSLIIVNFHRIFDQEIRSTFDKLFSSLKDFYQIKILKNLADCKLFEDFVKDLSSFSVVTISINTILVGALLEILFFFIFIIIIYANFYK
Sbjct:    1 MNGFQNDKNGGIFNYISKVINGSSYERLSESSTWVNYVKSIFVSKSSNKTYLTLAILLSLFFIFLSIIGVVLMRRCLRKLKANPSDLRLSKNFSRFIYFILGFLTISFILKVAFFSSTFSTNSTFYRSGNQSLLQFQSFINHSLIDFQKKINLNSLIENSTTIINSTIFAIMSRLMVDLQVKTLFDEMPDFIDNLMLVNNSFHRISLYSKQHHFQVLKSIGKLESISDLLSDKSLNLSRHKNCAEIDNLLQIITNNKPNTNLKFDSSIFITKVHELVPMEVDILKIKREFDEINRDMKKVAPEALADFKRDIPLDLIEVKKNQIFIMMSNSAVKIQKIKEKSSKNSKYIDTHLRSLEVFAIIIYTLINFFEYLIILKFTFNEKMSKTIVFFNLSNILMILFITVASVLLVFSFLILTAIYNESFRYFENENENLSIIDRILSTNINNIYIDIKTKIIENLGEYRAFIILPITLPNQVLGTIKRNCTHSKSIFTILKFDSIVDFEALKKSDFFQKLILKSKMSSDTKIINLLKKISIPDIKKEYENIIYDLLRKILNKNDNEINLKSVLGILQYINNTKQLMREIRASKCPKLTAIPTQNLFSGVDNVIFTNYKILQTLFKDFQAIERNLFLPEKFKKLFPDIYQSQLNIKNKSLIIVNFHRIFDQEIRSTFDKLFSSLKDFYQIKILKNLADCKLFEDFVKDLSSFSVVTISINTILVGALLEILFFFIFIIIIYANFYK 740
[Close]
BLAST of SMED30035012 vs. Planmine SMEST
Match: SMESG000049797.1 (SMESG000049797.1)

HSP 1 Score: 342.043 bits (876), Expect = 2.330e-105
Identity = 246/734 (33.51%), Postives = 409/734 (55.72%), Query Frame = 1 
Query:  271 MNGFQNDKNGGIFNYISKVINGSSYERLSESSTWVNYVKSIFVSKSSNKTYLTLAXXXXXXXXXXXXXGVV-------------LMRRCLRKLKANPSDLRLSKNFSRFIYFILGFLTISFILKVAXXXXXXXXXXXXYRSGNQSLLQFQSFINHSLIDFQKKINLXXXXXXXXXXXXXXXFAIMSRLMVDLQVKTLFDEMPDFIDNLMLVNNSFHRISLYSKQHHFQVLKSIGXXXXXXXXXXXXXXXXXRHKNCAEIDNLLQIITNNKPNTNLKFDSSIFITKVHELVPMEVDILKIKREFDEINRDMKKVAPEALADFKRDIPLDLIEVKKNQIFIMMSNSAVKIQKIKEKSSKNSKYIDTHLRSLEVFAIIIYTLINFFEYLIILKFTFNEKMSKTIVFFNLSNILMILFITVASVLLVFSFLILTAIYNESFRYFENENENLSIIDRILSTNINNIYIDIKTKIIENLGEYRAFIILPITLPNQVLGTIKRNCTHSKSIFTILKFDSIVDFEALKKSDFFQKLILKSKMSSDTKIINLLKKISIPDIKKEYENIIYDLLRKILNKNDNEINLKSVLGILQYINNTKQLMREIRASKCP----KLTAIPTQNLFSGVDNVIFTNYKILQTLFKDFQAIERNLFLPEKFKKLFPDIYQSQLNIKNKSLIIVNFHRIFDQEIRSTFDKLFSSLKDFYQIKILKNLADCKLFEDFVKDLSSFSVVTISINTIL 2421
            MN FQ ++NGGIF Y+S  +  S Y  LS  +   NY+ S     SSNK YL   IL ++ F    II ++               +RC+R  K+       SK   RF YF++  + I    +       F   +  +   NQS+   + FIN+SLID Q KI+L  +IE S   INSTI  I+S+L  DL +  LF  +P FID ++ +N S +RIS +++Q+  ++LK +GK+E IS+   ++  N +R + C++ +  L  +T NKP   LK +S IFI K+ E + + +D+ +IK+   ++++ +K +  + L DF   +P  +I  K  +I + MS S  KI K+ ++    S  I+  L  +++ A + Y +IN F+ ++I  FTF+ K  +  ++++   I  ++  +  SV+    F+ILT  YNE+FRYFEN+N N  I+D ILS  +N+ +     K    LG ++    +P  LP +++  IK +C  ++SI +IL F SIV+ + +++S+ ++++  + K  ++ K  +L++ +S+P I ++YE+II++ L KILN+ND E NL+++  IL Y    + L  EI  S C     ++  I  ++  S   N +    KI+  +++DFQ I+ NL LPE+F+K FP +  S  +++N++LII  FH IF  +I    +K+      FYQ  I  N+ DC +F  F   LS +  + +S+N +L
Sbjct:    1 MNNFQPNRNGGIFVYMSDYVIQSCYSCLSNRTIISNYLNSYLDLTSSNKFYLATTILATIIFTTFLIITIILIHSFIKKYIKSRFHKRCVRFRKS--LTFETSKFNRRFSYFMVSLIVIGMASRTVTIFPVFLEKTNVHEITNQSVFHLEEFINNSLIDSQNKIDLMPIIELSKKSINSTIIDIVSQLKTDLNIDFLFKSIPRFIDQIISINTSCYRISNFNQQNRTEILKFLGKIEFISNSFMEEERNANRFEECSKTNQTLFRLTRNKPIGELKIESLIFIKKLQETLDVNIDLNEIKKTLQDLSKTLKDLPSKTLTDFWATLPFGVINSKSTEISVQMSESYKKINKVSKEIVTFSGEINLILTIVKIVASVFYVVINIFQLVLIFAFTFDLKFKRNYLWYHSYQIFTMVISSFLSVVFFVCFIILTISYNEAFRYFENDNNNSKILDGILSQTVNSNWEQFAVKSGNFLGNFQTIFKIPTKLPEKLIRKIKTDCVTNQSILSILNFQSIVNIDLIRQSEIYRRIQFEIKSRANQKFDSLIRNLSVPKIDQKYESIIFNFLEKILNRNDTENNLQTIQSILNYFQTFQILTNEILDSNCTDFGNQIYRIFNKHFISD-KNQMKKFMKIVDLIYRDFQIIKNNLNLPERFRKSFPRVLASLNDLQNRNLIIETFHEIFRSKISRLSEKILILFDSFYQNLIFSNVGDCSIFMRFYSSLSGYPRIILSMNIML 731
The following BLAST results are available for this feature:
BLAST of SMED30035012 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035012 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035012 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035012 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035012 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035012 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035012 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035012 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035012 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035012 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035012 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035012 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035012 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30035012 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000049841.10.000e+0100.00SMESG000049841.1[more]
SMESG000049841.10.000e+0100.00SMESG000049841.1[more]
SMESG000049797.12.330e-10533.51SMESG000049797.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30035012 ID=SMED30035012|Name=SMED30035012|organism=Schmidtea mediterranea sexual|type=transcript|length=2495bp
ATAAAACCAATTTATACACCGTGTAATTTATTGCAAAATCTAGTTTTTAT
TATTGATTAAATAATCATCATCGTCACAATTTTTGATCTGTTACTAAATG
AACGAATCAAATTCAATAGCACTGTGCTATTTCATTTATTTAATGAGTAG
TATTTACTATTACTGTGAAAATTAATTAGTGATTGCTGATCAGTAACAAA
AAGCCTCGTAACCATTTAGCTCAATTTTTACGTCATCCAAAATCAAATGG
ATCGTTTAACAAAGTGCTTAATGAATGGCTTTCAAAACGATAAGAATGGC
GGGATATTCAACTACATTTCAAAAGTGATCAACGGATCATCCTATGAACG
ACTATCAGAATCGTCCACTTGGGTCAATTATGTTAAATCTATTTTCGTTT
CGAAATCTTCAAATAAAACTTATTTAACTCTAGCAATACTCTTATCTCTA
TTTTTTATTTTCTTATCGATAATTGGCGTTGTTTTAATGAGAAGGTGTCT
GAGAAAATTAAAAGCAAATCCGTCAGATTTACGCCTATCCAAAAATTTTA
GCAGATTCATATATTTTATTTTAGGTTTTTTAACAATAAGTTTTATATTA
AAAGTTGCATTTTTTTCATCGACGTTTTCCACGAATTCCACTTTCTACCG
TTCCGGGAATCAATCTTTATTACAATTCCAATCGTTCATAAATCATTCTT
TGATTGACTTTCAGAAAAAAATCAATTTAAATTCATTAATAGAAAACTCT
ACGACAATAATCAATTCGACAATTTTTGCAATTATGTCCCGGCTGATGGT
GGACCTTCAAGTGAAGACCTTATTTGATGAAATGCCAGATTTCATAGATA
ATCTCATGTTAGTCAATAATTCTTTTCATAGAATTTCTCTTTACAGCAAA
CAGCACCATTTTCAAGTGCTAAAATCAATCGGTAAACTCGAATCGATCTC
AGATCTTTTGTCCGATAAGAGTTTGAATTTGTCTCGGCACAAAAATTGTG
CTGAAATCGATAATTTATTACAAATTATTACAAACAACAAACCGAACACA
AATTTGAAATTTGATAGTTCGATTTTTATCACCAAAGTTCACGAATTAGT
CCCCATGGAGGTGGATATCCTCAAAATCAAAAGAGAATTCGATGAAATTA
ATAGAGACATGAAGAAAGTGGCACCTGAAGCTTTGGCTGATTTCAAAAGA
GATATTCCTTTAGATTTGATTGAAGTAAAGAAAAATCAAATTTTCATTAT
GATGTCCAACAGTGCTGTTAAAATCCAAAAGATAAAAGAAAAATCTTCAA
AGAATTCAAAATACATTGACACTCATTTGAGATCCTTAGAGGTCTTCGCT
ATTATAATCTACACCTTGATAAATTTCTTTGAATATCTTATCATTTTAAA
ATTCACTTTCAACGAAAAGATGTCAAAAACAATTGTTTTCTTTAATCTTT
CGAATATTTTGATGATTTTATTTATAACCGTTGCATCGGTTTTGTTAGTG
TTCTCCTTTCTCATACTAACCGCCATTTATAATGAAAGTTTCAGATATTT
TGAGAACGAAAATGAAAACCTTTCAATTATCGACAGGATCTTGTCAACAA
ATATTAACAACATATACATCGATATCAAAACGAAAATCATTGAGAATTTA
GGAGAATATCGAGCATTCATTATCTTACCAATAACTCTCCCAAATCAAGT
CTTGGGCACAATAAAACGTAATTGCACTCATTCTAAATCCATTTTCACTA
TTTTAAAATTCGATTCCATTGTCGATTTTGAAGCACTTAAAAAGTCTGAT
TTTTTTCAAAAGCTCATTTTGAAATCCAAAATGAGTTCTGACACAAAAAT
TATCAATTTATTAAAGAAAATATCGATTCCTGACATTAAAAAAGAATACG
AAAACATAATTTATGACTTACTTCGAAAGATTCTCAATAAAAATGACAAT
GAAATCAACCTGAAATCGGTTTTAGGAATTTTACAATATATCAACAACAC
AAAACAACTGATGCGTGAAATTCGGGCTTCCAAATGTCCAAAATTAACAG
CTATTCCCACACAAAACTTATTTTCGGGAGTGGACAATGTAATTTTCACA
AACTACAAAATACTTCAGACCCTGTTCAAAGATTTTCAAGCGATAGAGAG
AAATTTATTTTTACCCGAAAAATTTAAAAAACTTTTCCCCGATATTTATC
AATCTCAGCTGAACATCAAAAACAAAAGTTTAATTATCGTAAATTTCCAC
AGAATTTTCGATCAAGAAATCAGATCAACATTCGACAAACTGTTCAGCTC
TTTGAAGGACTTTTACCAGATAAAAATATTAAAAAATTTAGCAGATTGCA
AACTTTTTGAGGATTTTGTCAAAGATCTATCTAGTTTTTCTGTTGTGACG
ATATCTATTAATACTATTTTAGTCGGTGCACTTTTGGAGATTTTATTTTT
TTTTATATTTATAATTATAATTTATGCTAATTTTTACAAATAAAT
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protein sequence of SMED30035012-orf-1

>SMED30035012-orf-1 ID=SMED30035012-orf-1|Name=SMED30035012-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=749bp
MDRLTKCLMNGFQNDKNGGIFNYISKVINGSSYERLSESSTWVNYVKSIF
VSKSSNKTYLTLAILLSLFFIFLSIIGVVLMRRCLRKLKANPSDLRLSKN
FSRFIYFILGFLTISFILKVAFFSSTFSTNSTFYRSGNQSLLQFQSFINH
SLIDFQKKINLNSLIENSTTIINSTIFAIMSRLMVDLQVKTLFDEMPDFI
DNLMLVNNSFHRISLYSKQHHFQVLKSIGKLESISDLLSDKSLNLSRHKN
CAEIDNLLQIITNNKPNTNLKFDSSIFITKVHELVPMEVDILKIKREFDE
INRDMKKVAPEALADFKRDIPLDLIEVKKNQIFIMMSNSAVKIQKIKEKS
SKNSKYIDTHLRSLEVFAIIIYTLINFFEYLIILKFTFNEKMSKTIVFFN
LSNILMILFITVASVLLVFSFLILTAIYNESFRYFENENENLSIIDRILS
TNINNIYIDIKTKIIENLGEYRAFIILPITLPNQVLGTIKRNCTHSKSIF
TILKFDSIVDFEALKKSDFFQKLILKSKMSSDTKIINLLKKISIPDIKKE
YENIIYDLLRKILNKNDNEINLKSVLGILQYINNTKQLMREIRASKCPKL
TAIPTQNLFSGVDNVIFTNYKILQTLFKDFQAIERNLFLPEKFKKLFPDI
YQSQLNIKNKSLIIVNFHRIFDQEIRSTFDKLFSSLKDFYQIKILKNLAD
CKLFEDFVKDLSSFSVVTISINTILVGALLEILFFFIFIIIIYANFYK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 58..80
NoneNo IPR availableTMHMMTMhelixcoord: 401..423
NoneNo IPR availableTMHMMTMhelixcoord: 366..388
NoneNo IPR availableTMHMMTMhelixcoord: 724..746
NoneNo IPR availableTMHMMTMhelixcoord: 101..123