G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30034984
Length (bp)1093
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30034984) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30034984) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30034984) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30034984) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30034984

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30034984h1SMcG0007659 SmedASXL_005061SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A3S4ZJY0 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS6891 PE=4 SV=1)

HSP 1 Score: 197.593 bits (501), Expect = 8.017e-54
Identity = 138/371 (37.20%), Postives = 190/371 (51.21%), Query Frame = 1
Query:   40 YFFSWFLLLVNLVSLFGCCLCLGVILKYK-VFTKSTRIWFLALAASDISTILIATSDNYLALTFSN-FHLSTMKYLGFFGCQIRFGAIVAISWTCNILQSGFSIERSISIIKPLQARVFLTEMRSK------------------------------XXXXXXXXXXXXCGFSVTAVSMKFDTKT-----------SECLRKEYLGNVISITKIVYYTDLIVFRTXXXXXXXXXXXXXXXXXKFRTKRSVKYLQ----INRRTSAESNGKDSNISGRESQVFVLLLIMNFMALLTNPLFLIFELIDGEGSENKEFGKLSGDKCLSNILTTVFNCLMYTNNFTNWIFYIVIGTRFKRFTVAIISHWYQKLCLC 1011
            YF+ WF+ L N        LC  V+L+++ VF  STR WFLAL ASD   +L A +D Y  +TF + +H      LG + C IR   IV + W+ N+LQ+G S+ER ISI+ PL++R  LT   ++                                           G ++T + ++   K+           +EC R+    N+  ITKIVY+ DLIVFR IP   M+I S I+A  ++ +++R  + +     + RR  A        +  RES+  +LLL MN  ALLTNPLFLIFEL DGE SE     KL+GD C+S +LTT FN LMY NN TNWIFY+  G RF+R    ++      LC C
Sbjct:   21 YFYVWFMPLANHTGTICSGLCFVVLLRHRRVFASSTRAWFLALTASDFLILLFAGNDMYAEVTFPHAYHYFIGCVLGPYTCIIRLSTIVGLLWSSNLLQAGLSLERLISILAPLRSRALLTGRLARFIITGMISLALVGGIAMNMLSLQPTIFAQLANSTEEAIQVGPAGVNLTELDLEESPKSNDSGSFDYGLLAECRRR----NISHITKIVYHIDLIVFRAIPFATMLISSGILACLIRSQSRRRNELVHNLSLVQRRHRAV-------LQSRESRASLLLLAMNATALLTNPLFLIFELWDGEASEGHPAAKLTGDVCVSAMLTTFFNFLMYFNNQTNWIFYLTFGARFRRHIQDLV------LCRC 374          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A2H1CRY9 (Rhodopsin orphan GPCR OS=Fasciola hepatica OX=6192 GN=D915_001415 PE=4 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 4.209e-53
Identity = 133/349 (38.11%), Postives = 195/349 (55.87%), Query Frame = 1
Query:    1 NSSYFITFQLIKTYFFSWFLLLVNLVSLFGCCLCLGVILKY-KVFTKSTRIWFLALAASDISTILIATSDNYLALTFS-NFHLSTMKYLGFFGCQIRFGAIVAISWTCNILQSGFSIERSISIIKPLQARVFLTEMRSKXXXXXXXXXXXXCGFSVTAVSMKFDTKTSECLR-KEYLGNV--------------------ISITKIVYYTDLIVFRTXXXXXXXXXXXXXXXXXKFRTKRSVKYLQINRRTSAESNGKDSNISGRESQVFVLLLIMNFMALLTNPLFLIFELIDGEGSENKEFGKLSGDKCLSNILTTVFNCLMYTNNFTNWIFYIVIGTRFKRFTVAI 978
            +SS +I    I  YFFSW+++L NL +     LC  V++K+ +VF  STR WF+A+  SD  T+L+A +D Y  + F  ++H    K  G + C +R   I+++ W+ N+LQ+G S+ER  S+I PL+ R   T   ++  I+ +   +  CG ++    + +D K  E    +E  GN                     + +TKIVY+ DLI+FR IP   M++ S IIA  ++ + +R  K +  N           SN    ESQ  +LLL MN   L+TNPLFLIFELIDGEGSE +  GKL+G+ C++ +L  +FNCL+Y NN TNWIFYI  G RF+   V +
Sbjct:   10 DSSRYIADGFIN-YFFSWYIVLANLTATVFSGLCFFVLIKHPRVFASSTRAWFMAITVSDFFTLLLAANDMYAEVMFPMSYHRLLFKVFGRYNCMVRLSLILSLGWSSNLLQTGLSLERLASVIAPLKTRALTTGRLTRFTILGIIIFS--CGIAIGVNILSYDPKYMESGDTQEEPGNEMLLECDRVNGSTLLGEMNVNLDLTKIVYHIDLIMFRAIPFGTMLVSSAIIASLIRCQRRRRTKLMGANSVLQQRQLYSGSN---HESQASLLLLTMNLTTLITNPLFLIFELIDGEGSEGRTSGKLTGNTCVTWVLRQIFNCLLYFNNQTNWIFYIAFGARFRHHIVTL 352          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A504YS73 (Rhodopsin orphan GPCR OS=Fasciola gigantica OX=46835 GN=FGIG_02002 PE=4 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 1.011e-52
Identity = 136/349 (38.97%), Postives = 197/349 (56.45%), Query Frame = 1
Query:    1 NSSYFITFQLIKTYFFSWFLLLVNLVSLFGCCLCLGVILKY-KVFTKSTRIWFLALAASDISTILIATSDNYLALTFS-NFHLSTMKYLGFFGCQIRFGAIVAISWTCNILQSGFSIERSISIIKPLQARVFLTEMRSKXXXXXXXXXXXXCGFSVTAVSMKFDTKTSE---------------CLR---KEYLGNV---ISITKIVYYTDLIVFRTXXXXXXXXXXXXXXXXXKFRTKRSVKYLQINRRTSAESNGKDSNISGRESQVFVLLLIMNFMALLTNPLFLIFELIDGEGSENKEFGKLSGDKCLSNILTTVFNCLMYTNNFTNWIFYIVIGTRFKRFTVAI 978
            +SS +I    I +YFFSW+++L NL +     LC  V++K+ +VF  STR WF+A+  SD  T+L+A +D Y  + F  ++H       G + C +R   I+++ W+ N+LQ+G S+ER  S+I PL+ R   T   ++  I+ +   +  CG ++    + +D K  E               C R      LG +   + +TKIVY+ DLI+FR IP   M+I S IIA  ++ + KR  K +  N           SN   RESQ  +LLL MN   L+TNPLFLIFELIDGEGSE +  GKL+G+ C++ +L  +FNCL+Y NN TNWIFYI  G RF+   V +
Sbjct:   10 DSSRYIADGFI-SYFFSWYIVLANLTATVFSGLCFFVLIKHPRVFASSTRAWFMAITVSDFFTLLLAANDMYAEVMFPMSYHRLLFNVFGRYNCMVRLSLILSLGWSSNLLQTGLSLERLASVIAPLRTRALTTGRLTRFTILGIIIFS--CGIAIGVNILSYDPKYMESGETQKEPGNEMPLACDRVNGSTLLGEMNVNLDLTKIVYHIDLIMFRAIPFGTMLISSAIIASLIRCQRKRRTKLIGANSVLQQRQLYSGSN---RESQASLLLLTMNLTTLITNPLFLIFELIDGEGSEGRTSGKLTGNICVTWVLRQMFNCLLYFNNQTNWIFYIAFGARFRHHIVTL 352          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A430QH60 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma bovis OX=6184 GN=DC041_0001707 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 1.551e-49
Identity = 131/337 (38.87%), Postives = 188/337 (55.79%), Query Frame = 1
Query:   40 YFFSWFLLLVNLVSLFGCCLCLGVILKY-KVFTKSTRIWFLALAASDISTILIATSDNYLALTFSN-FHLSTMKYLGFFGCQIRFGAIVAISWTCNILQSGFSIERSISIIKPLQARVFLTEMRSKXXXXXXXXXXXXCGFSVTAVSMKFDTKT-------------------SECLRK---EYLGNV---ISITKIVYYTDLIVFRTXXXXXXXXXXXXXXXXXKFRTKRSVKYLQINRRTSAESNGKDSNIS---GRESQVFVLLLIMNFMALLTNPLFLIFELIDGEGSENKEFGKLSGDKCLSNILTTVFNCLMYTNNFTNWIFYIVIGTRFK 960
            YFFSW++++ N++ +    LC  V+LK+ +VF  +TR WF+ L ASD   +LIA +D Y  + F++ +H+      G + C IR   IV + WT N+LQ+G SIER  S+I PL+ R+  T   ++  I +L + + + G  +T VS K  +                       C R+   E  GN    I +TK+VY  DLIVFR IP + M+I S +IA  ++++ ++       NR T   S  +  N S    RES+  +LLL MN + LLTNPLFL+FEL DGEGSE +   KL+GD C+S IL   FN L+Y  N  NWI Y+  G RF+
Sbjct:   22 YFFSWYMIIANIIGIIFSGLCFLVLLKHPRVFASTTRAWFITLTASDFFILLIAANDMYAEVIFTHMYHIILGNIFGRYTCLIRVITIVCLLWTSNLLQTGLSIERLASVISPLKTRIIFTGKVTRYTISLLITFSCLSGILITIVSSKPSSSNIFMITNNNLTNYSIDYEALVSCERRNSTELFGNTHESIDLTKLVYNLDLIVFRVIPFITMLISSAVIASLVRYQRRKR------NRLTRISSIIQKRNRSIGYNRESRASLLLLTMNIITLLTNPLFLLFELFDGEGSEGRTAAKLTGDLCISWILRQFFNSLIYFGNQNNWILYLAFGARFR 352          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A094ZIN4 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma haematobium OX=6185 GN=MS3_02621 PE=4 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 1.997e-49
Identity = 131/337 (38.87%), Postives = 188/337 (55.79%), Query Frame = 1
Query:   40 YFFSWFLLLVNLVSLFGCCLCLGVILKY-KVFTKSTRIWFLALAASDISTILIATSDNYLALTFSN-FHLSTMKYLGFFGCQIRFGAIVAISWTCNILQSGFSIERSISIIKPLQARVFLTEMRSKXXXXXXXXXXXXCGFSVTAVSMKFDTKT-------------------SECLRK---EYLGNV---ISITKIVYYTDLIVFRTXXXXXXXXXXXXXXXXXKFRTKRSVKYLQINRRTSAESNGKDSNIS---GRESQVFVLLLIMNFMALLTNPLFLIFELIDGEGSENKEFGKLSGDKCLSNILTTVFNCLMYTNNFTNWIFYIVIGTRFK 960
            YFFSW++++ N++ +    LC  V+LK+ +VF  +TR WF+ L ASD   +LIA +D Y  + F++ +H+      G + C IR   IV + WT N+LQ+G SIER  S+I PL+ R+  T   ++  I +L + + + G  +T VS K  +                       C R+   E  GN    I +TK+VY  DLIVFR IP + M+I S +IA  ++++ ++       NR T   S  +  N S    RES+  +LLL MN + LLTNPLFL+FEL DGEGSE +   KL+GD C+S IL   FN L+Y  N  NWI Y+  G RF+
Sbjct:   22 YFFSWYMIIANIIGIIFSGLCFLVLLKHPRVFASTTRAWFITLTASDFFILLIAANDMYAEVIFTHMYHIILGNIFGRYTCLIRVITIVCLLWTSNLLQTGLSIERLASVISPLKTRIIFTGKVTRYTISLLITFSCLSGILITIVSSKPSSSNIFMITNNNITNYSIDYEALVSCERRNSTELFGNTHESIDLTKLVYNLDLIVFRVIPFITMLISSAVIASLVRYQRRKR------NRLTRISSIIQKRNRSIGYNRESRASLLLLTMNIITLLTNPLFLLFELFDGEGSEGRTAAKLTGDLCISWILRQFFNSLIYFGNQNNWILYLAFGARFR 352          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000055874.1 (SMESG000055874.1)

HSP 1 Score: 651.358 bits (1679), Expect = 0.000e+0
Identity = 356/356 (100.00%), Postives = 356/356 (100.00%), Query Frame = 1
Query:    1 NSSYFITFQLIKTYFFSWFLLLVNLVSLFGCCLCLGVILKYKVFTKSTRIWFLALAASDISTILIATSDNYLALTFSNFHLSTMKYLGFFGCQIRFGAIVAISWTCNILQSGFSIERSISIIKPLQARVFLTEMRSKXXXXXXXXXXXXCGFSVTAVSMKFDTKTSECLRKEYLGNVISITKIVYYTDLIVFRTXXXXXXXXXXXXXXXXXKFRTKRSVKYLQINRRTSAESNGKDSNISGRESQVFVLLLIMNFMALLTNPLFLIFELIDGEGSENKEFGKLSGDKCLSNILTTVFNCLMYTNNFTNWIFYIVIGTRFKRFTVAIISHWYQKLCLCFRFQTKMDAKVSFRSNNAV 1068
            NSSYFITFQLIKTYFFSWFLLLVNLVSLFGCCLCLGVILKYKVFTKSTRIWFLALAASDISTILIATSDNYLALTFSNFHLSTMKYLGFFGCQIRFGAIVAISWTCNILQSGFSIERSISIIKPLQARVFLTEMRSKLVIIILTSVTLICGFSVTAVSMKFDTKTSECLRKEYLGNVISITKIVYYTDLIVFRTIPLLIMIIGSFIIAISMKFRTKRSVKYLQINRRTSAESNGKDSNISGRESQVFVLLLIMNFMALLTNPLFLIFELIDGEGSENKEFGKLSGDKCLSNILTTVFNCLMYTNNFTNWIFYIVIGTRFKRFTVAIISHWYQKLCLCFRFQTKMDAKVSFRSNNAV
Sbjct:    8 NSSYFITFQLIKTYFFSWFLLLVNLVSLFGCCLCLGVILKYKVFTKSTRIWFLALAASDISTILIATSDNYLALTFSNFHLSTMKYLGFFGCQIRFGAIVAISWTCNILQSGFSIERSISIIKPLQARVFLTEMRSKLVIIILTSVTLICGFSVTAVSMKFDTKTSECLRKEYLGNVISITKIVYYTDLIVFRTIPLLIMIIGSFIIAISMKFRTKRSVKYLQINRRTSAESNGKDSNISGRESQVFVLLLIMNFMALLTNPLFLIFELIDGEGSENKEFGKLSGDKCLSNILTTVFNCLMYTNNFTNWIFYIVIGTRFKRFTVAIISHWYQKLCLCFRFQTKMDAKVSFRSNNAV 363          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000009916.1 (SMESG000009916.1)

HSP 1 Score: 254.603 bits (649), Expect = 7.742e-82
Identity = 146/324 (45.06%), Postives = 212/324 (65.43%), Query Frame = 1
Query:    1 NSSYFITFQLIKTYFFSWFLLLVNLVSLFGCCLCLGVILKYKVFTKSTRIWFLALAASDISTILIATSDNYLALTFS-NFHLSTMKYLGFFGCQIRFGAIVAISWTCNILQSGFSIERSISIIKPLQARVFLTEMRSKXXXXXXXXXXXXCGFSVTAVSMKFDTKT---SECLRKEYLGNVISITKIVYYTDLIVFRTXXXXXXXXXXXXXXXXXKFRTKRSVKYLQINRRTSAESNGKDSNISGRESQVFVLLLIMNFMALLTNPLFLIFELIDGEGSENKEFGKLSGDKCLSNILTTVFNCLMYTNNFTNWIFYIVIGTRFK 960
            NSS+++ F+  + Y  SWFL+ +NL ++FG  LC+ VI+KY VF+ +T+ WFLAL  SDI  I +A +D Y  L F+  + L  + +  FFGCQ R+G +VA+SW CN LQ+G S+ER+I II P++ARVF T  RS+++II L  ++ + G  +   +++    +   S C+RK         TK VY+ DLI+FR +P  ++ I + I+ I +K R +   K+     R+S    GK+      E ++F+LL++MN + L TNPLFL+FELIDGE +EN  FGKL+GDKCLS ILT+ FN LMY NN TNWIFYI +G++F+
Sbjct:    8 NSSHWMVFEYFQYYILSWFLITINLSAIFGSGLCICVIVKYNVFSVNTKYWFLALIISDIVIIFVAANDFYFTLIFNEQYKLFLLHFSAFFGCQFRYGLVVAVSWVCNALQTGLSLERTIHIISPIKARVFFTVKRSRIIIIFLVIISGMSGLIINICTLEVKPTSKNVSSCVRKP------DQTKFVYFIDLILFRALPYGVITITTVILIIFIK-RRQNCRKFTL--HRSSRTPGGKNELNLNPERRMFILLIVMNVITLTTNPLFLLFELIDGEATENMVFGKLTGDKCLSTILTSFFNELMYMNNLTNWIFYINVGSKFR 322          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000026034.1 (SMESG000026034.1)

HSP 1 Score: 210.305 bits (534), Expect = 3.765e-64
Identity = 125/318 (39.31%), Postives = 195/318 (61.32%), Query Frame = 1
Query:   40 YFFSWFLLLVNLVSLFGCCLCLGVILKYKVFTKSTRIWFLALAASDISTILIATSDNYLALTFSNFHLSTMKYLGFFGCQIRFGAIVAISWTCNILQSGFSIERSISIIKPLQARVFLTEMRSKXXXXXXXXXXXXCGFSVTAVSM-KFDTKTSECLRKEYLGNVISITKIVYYTDLIVFRTXXXXXXXXXXXXXXXXXKFRTKRSVKYLQINRRTSAESNGKDSNISGRESQVFVLLLIMNFMALLTNPLFLIFELIDGEGSENKEFGKLSGDKCLSNILTTVFNCLMYTNNFTNWIFYIVIGTRFKRFTVAIISHW 990
            Y FSW   L++L  +FG  +C+ VI+K++VF   T+ WF+ L+ SD   ++I   D Y       +++ T+  +  FGC++R+ AI   SW  N+LQ+G SIER+IS++ P+++R+  T  R+K    I++ +   C  S + + M  F+     C RK +  + I +T  +Y  DLI+FR IP L+M+I S II I MK     S+K  +I        N K S    RE ++ V+L+ MN   L+TNPLFLIFELIDG GSE +++GKL+ ++C+S+    +FN L+Y NN TNWIFY++IG +F+ + + I+S++
Sbjct:   21 YCFSWSFALLSLAGIFGSLMCIIVIVKHRVFIHGTKYWFIYLSMSDFLMLIIPACDLYFYYMLEEYYIKTLNRMSIFGCELRYFAINFFSWNANVLQAGLSIERTISVLNPIRSRIIFTTGRTK---FIISVLIGFCFVSSSVIDMLTFERVNGTCRRKTHPNSSIDVTHSIYTIDLILFRAIPFLVMLISSIIIVILMKL---HSIKRDRIGHHQMGNPNKKSSQ-KYREFKMSVILISMNVATLVTNPLFLIFELIDGAGSEGQKYGKLTKNRCVSDFFNLLFNSLIYFNNQTNWIFYVLIGAKFQHYFLMILSNF 331          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000066767.1 (SMESG000066767.1)

HSP 1 Score: 190.274 bits (482), Expect = 4.980e-57
Identity = 124/350 (35.43%), Postives = 206/350 (58.86%), Query Frame = 1
Query:   25 QLIKTYFFSWFLLLVNLVSLFGCCLCLGVILKYKVFTKSTRIWFLALAASDISTILIATSDNYLALTFSNFHLSTMKYLGFFGCQIRFGAIVAISWTCNILQSGFSIERSISIIKPLQARVFLTEMRSKXXXXXXXXXXXXCGFSVTAVSMKFDTKTSECLRKEYLGNVISITKIVYYTDLIVFRTXXXXXXXXXXXXXXXXXK-FRTKRSVKYLQINRRTSAESNGKDSNISGRESQVFVLLLIMNFMALLTNPLFLIFELIDGEGSENKEFGKLSGDKCLSNILTTVFNCLMYTNNFTNWIFYIVIGTRFKRFTVAIISHWYQKLCLCFRFQTKMDAK-VSFRSNNAV 1068
            ++   Y  SW  L++ +  L G  LCL V+LKY VF+K+T+ WF +L  +DI  +L+   D Y  +     + S +   G FGC+IR+  I ++SW  N+LQS  SIERS S+++P++AR+  T+ ++K +I+I+   +L+ G     ++ +   + +  +         +I+ I+Y  DL +FR IP +IM   S+II I ++  + KR+   ++I R + A S         +E ++FVLL+ MN + LLTNPLF +FE IDG G+EN  +G  +GD C+S +L  +FNC +Y NN +NWIFYI  G +F+R+++ ++        + FRF +K + + + +  NN +
Sbjct:   19 EIFSQYILSWLFLILTIAGLCGNVLCLSVVLKYPVFSKNTKSWFCSLLIADILILLLPAMDLYFHVLLPELYPSLIARSGVFGCEIRYALIQSLSWLANLLQSSLSIERSYSVLRPMKARMLFTKTKTKSMILIIVMFSLVSGVLTNGLTFQRIPQGNSTICDRVRIERFNISHIIYNIDLFIFRVIPFIIMFTSSWIIIIELRCCKQKRNESNIRI-RNSKATSK--------KEFKMFVLLISMNVLTLLTNPLFALFETIDGVGTENYPYGLWTGDICMSKLLRLIFNCFIYLNNQSNWIFYIATGGKFRRYSLNVLR-------VIFRFHSKGNHESLRYSKNNQL 352          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000066767.1 (SMESG000066767.1)

HSP 1 Score: 190.274 bits (482), Expect = 5.142e-57
Identity = 124/350 (35.43%), Postives = 206/350 (58.86%), Query Frame = 1
Query:   25 QLIKTYFFSWFLLLVNLVSLFGCCLCLGVILKYKVFTKSTRIWFLALAASDISTILIATSDNYLALTFSNFHLSTMKYLGFFGCQIRFGAIVAISWTCNILQSGFSIERSISIIKPLQARVFLTEMRSKXXXXXXXXXXXXCGFSVTAVSMKFDTKTSECLRKEYLGNVISITKIVYYTDLIVFRTXXXXXXXXXXXXXXXXXK-FRTKRSVKYLQINRRTSAESNGKDSNISGRESQVFVLLLIMNFMALLTNPLFLIFELIDGEGSENKEFGKLSGDKCLSNILTTVFNCLMYTNNFTNWIFYIVIGTRFKRFTVAIISHWYQKLCLCFRFQTKMDAK-VSFRSNNAV 1068
            ++   Y  SW  L++ +  L G  LCL V+LKY VF+K+T+ WF +L  +DI  +L+   D Y  +     + S +   G FGC+IR+  I ++SW  N+LQS  SIERS S+++P++AR+  T+ ++K +I+I+   +L+ G     ++ +   + +  +         +I+ I+Y  DL +FR IP +IM   S+II I ++  + KR+   ++I R + A S         +E ++FVLL+ MN + LLTNPLF +FE IDG G+EN  +G  +GD C+S +L  +FNC +Y NN +NWIFYI  G +F+R+++ ++        + FRF +K + + + +  NN +
Sbjct:   29 EIFSQYILSWLFLILTIAGLCGNVLCLSVVLKYPVFSKNTKSWFCSLLIADILILLLPAMDLYFHVLLPELYPSLIARSGVFGCEIRYALIQSLSWLANLLQSSLSIERSYSVLRPMKARMLFTKTKTKSMILIIVMFSLVSGVLTNGLTFQRIPQGNSTICDRVRIERFNISHIIYNIDLFIFRVIPFIIMFTSSWIIIIELRCCKQKRNESNIRI-RNSKATSK--------KEFKMFVLLISMNVLTLLTNPLFALFETIDGVGTENYPYGLWTGDICMSKLLRLIFNCFIYLNNQSNWIFYIATGGKFRRYSLNVLR-------VIFRFHSKGNHESLRYSKNNQL 362          
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3S4ZJY08.017e-5437.20G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A2H1CRY94.209e-5338.11Rhodopsin orphan GPCR OS=Fasciola hepatica OX=6192... [more]
A0A504YS731.011e-5238.97Rhodopsin orphan GPCR OS=Fasciola gigantica OX=468... [more]
A0A430QH601.551e-4938.87G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A094ZIN41.997e-4938.87G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000055874.10.000e+0100.00SMESG000055874.1[more]
SMESG000009916.17.742e-8245.06SMESG000009916.1[more]
SMESG000026034.13.765e-6439.31SMESG000026034.1[more]
SMESG000066767.14.980e-5735.43SMESG000066767.1[more]
SMESG000066767.15.142e-5735.43SMESG000066767.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034984 ID=SMED30034984|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1093bp
AATTCATCGTATTTCATCACTTTTCAACTGATAAAAACGTATTTCTTTTC
CTGGTTTCTGTTACTTGTAAATCTAGTTTCTCTATTTGGTTGTTGTCTTT
GTTTGGGAGTGATTTTGAAGTATAAGGTCTTCACAAAGTCTACAAGGATC
TGGTTCCTAGCGTTGGCAGCTAGTGACATTTCGACGATTTTGATTGCAAC
ATCTGATAACTATTTAGCGTTGACTTTTAGTAACTTTCACTTATCAACTA
TGAAATATTTGGGATTTTTTGGCTGTCAAATACGATTCGGTGCAATCGTT
GCCATTTCATGGACATGCAACATTCTTCAGTCGGGATTCAGTATTGAAAG
AAGTATTAGCATTATCAAACCTCTTCAGGCACGAGTGTTTTTAACTGAGA
TGCGCAGCAAACTCGTTATAATAATTTTGACGTCTGTAACATTGATATGC
GGTTTTTCAGTGACTGCAGTTTCTATGAAGTTCGACACTAAAACCTCGGA
GTGCTTGAGGAAAGAATACTTGGGAAATGTAATTTCTATAACTAAAATTG
TATATTATACGGATCTAATTGTGTTTCGGACGATTCCTTTGCTTATTATG
ATAATTGGATCGTTTATAATAGCCATTTCAATGAAATTCAGAACAAAACG
ATCGGTGAAGTATTTACAAATCAACCGGCGGACAAGTGCCGAAAGTAACG
GCAAAGACTCGAATATTTCTGGACGAGAATCTCAGGTATTTGTTTTGTTG
TTGATCATGAATTTTATGGCTTTGCTCACGAATCCACTGTTCCTCATTTT
TGAATTAATCGACGGAGAAGGATCTGAAAATAAAGAATTCGGAAAATTAT
CAGGTGATAAATGCCTATCAAATATTCTAACAACTGTTTTTAATTGCTTG
ATGTACACGAACAATTTCACCAATTGGATATTTTACATTGTGATTGGGAC
GCGATTTAAGCGTTTTACGGTGGCGATTATCTCGCATTGGTATCAGAAAT
TATGTTTGTGTTTCAGATTTCAAACTAAAATGGATGCCAAAGTGTCATTC
CGATCAAATAACGCGGTGTGAATTTATTGAATTTGATCCGATC
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protein sequence of SMED30034984-orf-1

>SMED30034984-orf-1 ID=SMED30034984-orf-1|Name=SMED30034984-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=357bp
NSSYFITFQLIKTYFFSWFLLLVNLVSLFGCCLCLGVILKYKVFTKSTRI
WFLALAASDISTILIATSDNYLALTFSNFHLSTMKYLGFFGCQIRFGAIV
AISWTCNILQSGFSIERSISIIKPLQARVFLTEMRSKLVIIILTSVTLIC
GFSVTAVSMKFDTKTSECLRKEYLGNVISITKIVYYTDLIVFRTIPLLIM
IIGSFIIAISMKFRTKRSVKYLQINRRTSAESNGKDSNISGRESQVFVLL
LIMNFMALLTNPLFLIFELIDGEGSENKEFGKLSGDKCLSNILTTVFNCL
MYTNNFTNWIFYIVIGTRFKRFTVAIISHWYQKLCLCFRFQTKMDAKVSF
RSNNAV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 6..327
e-value: 7.8E-25
score: 89.5
NoneNo IPR availablePANTHERPTHR24243G-PROTEIN COUPLED RECEPTORcoord: 13..326
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 14..321
e-value: 7.04724E-11
score: 60.3392
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 7..333
NoneNo IPR availableTMHMMTMhelixcoord: 138..157
NoneNo IPR availableTMHMMTMhelixcoord: 87..109
NoneNo IPR availableTMHMMTMhelixcoord: 248..270
NoneNo IPR availableTMHMMTMhelixcoord: 15..37
NoneNo IPR availableTMHMMTMhelixcoord: 50..72
NoneNo IPR availableTMHMMTMhelixcoord: 189..211
NoneNo IPR availableTMHMMTMhelixcoord: 292..314
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 33..312
e-value: 2.8E-9
score: 36.7
IPR000276G protein-coupled receptor, rhodopsin-likePROSITEPS00237G_PROTEIN_RECEP_F1_1coord: 105..121
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 30..312
score: 15.938