SCP domain-containing protein

Overview
NameSCP domain-containing protein
Smed IDSMED30034978
Length (bp)1151
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SCP domain-containing protein (SMED30034978) t-SNE clustered cells

Violin plots show distribution of expression levels for SCP domain-containing protein (SMED30034978) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SCP domain-containing protein (SMED30034978) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SCP domain-containing protein (SMED30034978) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30034978

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30034978h1SMcG0003846 SmedASXL_014797SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parenchymaSMED30034978h1SMcG0003846 dd_Smed_v4_1141_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30034978h1SMcG0003846 dd_Smed_v6_1141_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30034978h1SMcG0003846 OX_Smed_1.0.02514ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SCP domain-containing protein vs. Ensembl Human
Match: PI16 (peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGNC:21245])

HSP 1 Score: 64.6994 bits (156), Expect = 1.053e-10
Identity = 53/194 (27.32%), Postives = 86/194 (44.33%), Query Frame = 2
Query:  308 APTSSISD--VKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCT-SKYCNGYKQLVNARTTEIGCAVSNCKK----GETFADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDS 856
             P  +++D   +L+++L N  R  +   A  M  +RW+ ELA  A+  + QC  G +           G+      +   + +     + E+++++  YN    TC+  + C  Y Q+V A+T  IGC    C+K     ET  +   CN+ PP  +   RPY++G T C  C        Y C   LCE   S
Sbjct:   23 GPVGALTDEEKRLMVELHNLYRAQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKER--------GRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEG-TPCSQCPS-----GYHCKNSLCEPIGS 202          
BLAST of SCP domain-containing protein vs. Ensembl Human
Match: PI16 (peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGNC:21245])

HSP 1 Score: 64.6994 bits (156), Expect = 1.053e-10
Identity = 53/194 (27.32%), Postives = 86/194 (44.33%), Query Frame = 2
Query:  308 APTSSISD--VKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCT-SKYCNGYKQLVNARTTEIGCAVSNCKK----GETFADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDS 856
             P  +++D   +L+++L N  R  +   A  M  +RW+ ELA  A+  + QC  G +           G+      +   + +     + E+++++  YN    TC+  + C  Y Q+V A+T  IGC    C+K     ET  +   CN+ PP  +   RPY++G T C  C        Y C   LCE   S
Sbjct:   23 GPVGALTDEEKRLMVELHNLYRAQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKER--------GRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEG-TPCSQCPS-----GYHCKNSLCEPIGS 202          
BLAST of SCP domain-containing protein vs. Ensembl Human
Match: PI16 (peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGNC:21245])

HSP 1 Score: 64.6994 bits (156), Expect = 1.053e-10
Identity = 53/194 (27.32%), Postives = 86/194 (44.33%), Query Frame = 2
Query:  308 APTSSISD--VKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCT-SKYCNGYKQLVNARTTEIGCAVSNCKK----GETFADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDS 856
             P  +++D   +L+++L N  R  +   A  M  +RW+ ELA  A+  + QC  G +           G+      +   + +     + E+++++  YN    TC+  + C  Y Q+V A+T  IGC    C+K     ET  +   CN+ PP  +   RPY++G T C  C        Y C   LCE   S
Sbjct:   23 GPVGALTDEEKRLMVELHNLYRAQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKER--------GRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEG-TPCSQCPS-----GYHCKNSLCEPIGS 202          
BLAST of SCP domain-containing protein vs. Ensembl Human
Match: GLIPR1L1 (GLIPR1 like 1 [Source:HGNC Symbol;Acc:HGNC:28392])

HSP 1 Score: 60.8474 bits (146), Expect = 5.339e-10
Identity = 58/198 (29.29%), Postives = 90/198 (45.45%), Query Frame = 2
Query:  296 CQVAAPTS---SISDVKLI---LDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGS----DNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKK-GETFADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCE 844
            C VA  +S   SI+D   I   ++  N  R K   PA  M  + W+  LA+ A+  +NQC+       D +  C +A     +         +S      I  +YN+   Y++D+ +C S+ C  Y QLV A +  +GCAV+ C   G      + CN+ P        PY +GE+ C  C K +     +C K+LC+
Sbjct:   16 CLVATTSSKIPSITDPHFIDNCIEAHNEWRGKVNPPAADMKYMIWDKGLAKMAKAWANQCKFEHNDCLDKSYKCYAA--FEYVGENIWLGGIKSFTPRHAITAWYNETQFYDFDSLSC-SRVCGHYTQLVWANSFYVGCAVAMCPNLGGASTAIFVCNYGPAGNFANMPPYVRGES-CSLCSKEE-----KCVKNLCK 204          
BLAST of SCP domain-containing protein vs. Ensembl Human
Match: GLIPR1L1 (GLIPR1 like 1 [Source:HGNC Symbol;Acc:HGNC:28392])

HSP 1 Score: 60.077 bits (144), Expect = 8.552e-10
Identity = 58/198 (29.29%), Postives = 89/198 (44.95%), Query Frame = 2
Query:  296 CQVAAPTS---SISDVKLI---LDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGS----DNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKK-GETFADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCE 844
            C VA  +S   SI+D   I   ++  N  R K   PA  M  + W+  LA+ A+  +NQC+       D +  C +A     +         +S      I  +YN+   Y++D+ +C S+ C  Y QLV A +  +GCAV+ C   G      + CN+ P        PY +GE+ C  C K +     +C K+LC 
Sbjct:   16 CLVATTSSKIPSITDPHFIDNCIEAHNEWRGKVNPPAADMKYMIWDKGLAKMAKAWANQCKFEHNDCLDKSYKCYAA--FEYVGENIWLGGIKSFTPRHAITAWYNETQFYDFDSLSC-SRVCGHYTQLVWANSFYVGCAVAMCPNLGGASTAIFVCNYGPAGNFANMPPYVRGES-CSLCSKEE-----KCVKNLCR 204          
BLAST of SCP domain-containing protein vs. Ensembl Fly
Match: CG8483 (gene:FBgn0038126 transcript:FBtr0082782)

HSP 1 Score: 49.6766 bits (117), Expect = 2.469e-6
Identity = 41/153 (26.80%), Postives = 67/153 (43.79%), Query Frame = 2
Query:  380 ALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQ---ISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETFADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYR 823
            A  M +I W+ ELA RAQ  ++ C+   D        + MGQ   I ++T   +     +   I  ++N+   Y++      S     Y QLV   T+ +GC  +  K    +   Y CN+ P   +  + PYE G+  C S Y +  +  Y+
Sbjct:   62 AENMREIVWDDELAARAQKWADNCQFRHDPHRTINRFT-MGQNLAIIWSTAPLDADDGDFPSRIQSWFNEVQKYSF--GDAWSPKTGHYSQLVWGETSLVGCGYAEYKDTSKYNKLYVCNYGPGGNVVGYNPYEVGKPSC-STYGMKPSSRYQ 210          
BLAST of SCP domain-containing protein vs. Ensembl Zebrafish
Match: crispld1b (cysteine-rich secretory protein LCCL domain containing 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1204])

HSP 1 Score: 67.3958 bits (163), Expect = 7.768e-12
Identity = 56/193 (29.02%), Postives = 90/193 (46.63%), Query Frame = 2
Query:  320 SISDVKLILDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYD---------TNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF------ADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLC 841
            S SD++LILDL N +R +   PA  M  + W+TEL + A++ ++ C      ++L T    +GQ   A    ++     T  +  +Y++   ++Y             C+   C  Y QLV A + +IGCA++ C     +      A Y  CN+ PP   + H PY+ G T C +C     +Y   C  +LC
Sbjct:   59 SQSDMQLILDLHNKLRGQVYPPASNMEYMVWDTELERSAEHWAHTCLWEHGPSHLLTR---IGQNLGAHWGRDRPP---TFHVQAWYDEVRDFSYPYPQECNPHCPYRCSGPVCTHYTQLVWATSNKIGCAINVCYNMNVWGMIWAKAVYLVCNYSPPGNWWGHAPYKHG-TPCSAC---PPSYGGGCRNNLC 241          
BLAST of SCP domain-containing protein vs. Ensembl Zebrafish
Match: CU914487.1 (peptidase inhibitor 16-like [Source:NCBI gene;Acc:101883528])

HSP 1 Score: 56.225 bits (134), Expect = 3.638e-9
Identity = 35/88 (39.77%), Postives = 48/88 (54.55%), Query Frame = 2
Query:  551 TQFILEFYNQKSGYNYDTNTCTS-KYCNGYKQLVNARTTEIGCAVSNCKKGETF----ADYYFCNFYPP--IYQHRPYEKGETKCDSC 793
            T+ +++++N+   YNY+TN C   K C  Y QLV A TT+IGCA   C   E      A    C++YP   I   +PYE GE+ C  C
Sbjct:   51 TKAVMDWFNENLDYNYNTNDCAEDKMCGHYTQLVWANTTKIGCASYFCDTLEKLHFEKATLLICDYYPQGNIEGQKPYESGES-CSKC 137          
BLAST of SCP domain-containing protein vs. Ensembl Zebrafish
Match: crispld1a (cysteine-rich secretory protein LCCL domain containing 1a [Source:ZFIN;Acc:ZDB-GENE-090612-1])

HSP 1 Score: 53.9138 bits (128), Expect = 1.982e-7
Identity = 57/227 (25.11%), Postives = 96/227 (42.29%), Query Frame = 2
Query:  320 SISDVKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTN---------TCTSKYCNGYKQLVNARTTEIGCAVSNCKK----GETFAD--YYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLC-----EICDSQSSD-CQGKEVLKLAIEAGLCEDL 925
            + SD++ ILDL N +R  +  PA  M  + W+ EL + A+  +  C        L      + QI         +    T  +  +Y++   Y++             C+   C  Y QLV A ++ IGCA++ C      G+ +A   Y  CN+ P    + + PY+ G T C +C     +Y   C ++LC       CD++  D C+G    +    AG  + +
Sbjct:   63 TASDMQAILDLHNKLRGQVYPPASNMEYMVWDNELERSAEEWAETCLWEHGPAGL------LPQIGQNLGVHWGRYRPPTSHVQAWYDEVKDYSFPYPQECNPHCPFRCSGPVCTHYTQLVWATSSRIGCAINVCYNMNVWGQIWAKAVYLVCNYSPKGNWWGYAPYKHG-TSCSAC---PPSYGGVCRENLCYKAQTVTCDTKLRDQCKGTTCNRYECPAGCLDSM 279          
BLAST of SCP domain-containing protein vs. Ensembl Zebrafish
Match: crispld1a (cysteine-rich secretory protein LCCL domain containing 1a [Source:ZFIN;Acc:ZDB-GENE-090612-1])

HSP 1 Score: 53.5286 bits (127), Expect = 2.501e-7
Identity = 52/207 (25.12%), Postives = 88/207 (42.51%), Query Frame = 2
Query:  320 SISDVKLILDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTN---------TCTSKYCNGYKQLVNARTTEIGCAVSNCKK----GETFAD--YYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDSQSSDCQGKE 883
            + SD++ ILDL N +R +   PA  M  + W+ EL + A+  +  C        L      + QI         +    T  +  +Y++   Y++             C+   C  Y QLV A ++ IGCA++ C      G+ +A   Y  CN+ P    + + PY+ G T C +C     +Y   C ++LC   D ++     +E
Sbjct:   63 TASDMQAILDLHNKLRGQVYPPASNMEYMVWDNELERSAEEWAETCLWEHGPAGL------LPQIGQNLGVHWGRYRPPTSHVQAWYDEVKDYSFPYPQECNPHCPFRCSGPVCTHYTQLVWATSSRIGCAINVCYNMNVWGQIWAKAVYLVCNYSPKGNWWGYAPYKHG-TSCSAC---PPSYGGVCRENLCYKGDGKNRQSPSEE 259          
BLAST of SCP domain-containing protein vs. Ensembl Zebrafish
Match: glipr1a (GLI pathogenesis-related 1a [Source:ZFIN;Acc:ZDB-GENE-110309-2])

HSP 1 Score: 48.521 bits (114), Expect = 4.592e-6
Identity = 56/235 (23.83%), Postives = 93/235 (39.57%), Query Frame = 2
Query:  356 NNIRMKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSA------SPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCT-SKYCNGYKQLVNARTTEIGCAVSNCKKGETFADYYFCNFYPPIY-----------QHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDSQSSDCQGKEVLKLAIEAGLCEDLEPGFCELNASVCPNIDLLPDEYKVMMT 1006
            N   +   A  M  + W+  LA  A+  +  C L   N +L  +       + +G+  +A     + ++K    +  + N+   YNY+ N C   K C  Y Q+V A + ++GCAV  C  G   A+ +F N    I+              PY++G + C  C   D     +C ++LC  C  +      +  L L I   L          L+ S   N D   ++ K  +T
Sbjct:   42 NRSSVSPTAANMRYMTWDAALAVTARAWARFC-LFKHNIHLREAKRVHPTFTTVGENIWAGAPYSRFTVK--SAVFSWVNELKDYNYNNNQCNDKKVCGHYTQVVWADSYKVGCAVQTCPNG--VAETHFSNIQGVIFVCNYATAGNFAGRSPYKQGAS-CSGCGGSD-----KCERNLCRKCKKK------QHTLHLIISCTL---------HLSTSSAGNTDRDAEQSKTHLT 250          
BLAST of SCP domain-containing protein vs. Ensembl Xenopus
Match: CRISPLD1 (cysteine-rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:100490431])

HSP 1 Score: 57.3806 bits (137), Expect = 1.841e-8
Identity = 52/191 (27.23%), Postives = 80/191 (41.88%), Query Frame = 2
Query:  326 SDVKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTN---------TCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF------ADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLC 841
            SD+KLILDL N +R  +  PA  M  + W+ EL + A+  +  C       +L      +GQ   A     +     T  +  +Y++   Y +             C+   C  Y QLV A ++ IGCA++ C     +      A Y  CN+ P    + H PY+ G   C +C     +Y   C  +LC
Sbjct:   60 SDMKLILDLHNKLRGEVYPPASNMEFMIWDVELERSAEAWAETCLWEHGPADLLPV---IGQNLGAHWGRYRPP---TYHVQAWYDEVRDYTFPYPQECDPYCPFRCSGPVCTHYTQLVWATSSRIGCAINLCHNMNVWGQIWPKAIYLVCNYSPKGNWWGHAPYKHGH-PCSAC---PPSYGGGCKDNLC 240          
BLAST of SCP domain-containing protein vs. Ensembl Xenopus
Match: CRISPLD1 (cysteine-rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:100490431])

HSP 1 Score: 56.6102 bits (135), Expect = 4.027e-8
Identity = 52/191 (27.23%), Postives = 80/191 (41.88%), Query Frame = 2
Query:  326 SDVKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTN---------TCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF------ADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLC 841
            SD+KLILDL N +R  +  PA  M  + W+ EL + A+  +  C       +L      +GQ   A     +     T  +  +Y++   Y +             C+   C  Y QLV A ++ IGCA++ C     +      A Y  CN+ P    + H PY+ G   C +C     +Y   C  +LC
Sbjct:   60 SDMKLILDLHNKLRGEVYPPASNMEFMIWDVELERSAEAWAETCLWEHGPADLLPV---IGQNLGAHWGRYRPP---TYHVQAWYDEVRDYTFPYPQECDPYCPFRCSGPVCTHYTQLVWATSSRIGCAINLCHNMNVWGQIWPKAIYLVCNYSPKGNWWGHAPYKHGH-PCSAC---PPSYGGGCKDNLC 240          
BLAST of SCP domain-containing protein vs. Ensembl Xenopus
Match: CRISPLD1 (cysteine-rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:100490431])

HSP 1 Score: 55.8398 bits (133), Expect = 5.878e-8
Identity = 52/191 (27.23%), Postives = 80/191 (41.88%), Query Frame = 2
Query:  326 SDVKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTN---------TCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF------ADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLC 841
            SD+KLILDL N +R  +  PA  M  + W+ EL + A+  +  C       +L      +GQ   A     +     T  +  +Y++   Y +             C+   C  Y QLV A ++ IGCA++ C     +      A Y  CN+ P    + H PY+ G   C +C     +Y   C  +LC
Sbjct:   60 SDMKLILDLHNKLRGEVYPPASNMEFMIWDVELERSAEAWAETCLWEHGPADLLPV---IGQNLGAHWGRYRPP---TYHVQAWYDEVRDYTFPYPQECDPYCPFRCSGPVCTHYTQLVWATSSRIGCAINLCHNMNVWGQIWPKAIYLVCNYSPKGNWWGHAPYKHGH-PCSAC---PPSYGGGCKDNLC 240          
BLAST of SCP domain-containing protein vs. Ensembl Xenopus
Match: R3HDML (R3H domain containing-like [Source:NCBI gene;Acc:100492715])

HSP 1 Score: 54.6842 bits (130), Expect = 5.917e-8
Identity = 44/155 (28.39%), Postives = 70/155 (45.16%), Query Frame = 2
Query:  320 SISDVKLILDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYD---------TNTCTSKYCNGYKQLVNARTTEIGCAVSNCKK----GETF--ADYYFCNF 733
            S  D+  +LD  N +R K   PA  M  + W+  LA+ A+  +NQC+       L      +GQ + +  S   +S+     +  +Y+++  Y++           N CT   C  Y Q+V A +  IGCAV+ C      G T+  A Y  CN+
Sbjct:   59 SPRDMSALLDYHNQVRSKVFPPAANMEYMVWDERLAKSAESWANQCKWDHGPNQLMRY---IGQ-NLSVHSGRYRSI--VDLVKGWYDERQHYSFPHPRECNPSCPNKCTGAVCTHYTQMVWASSNRIGCAVNICTNINVWGSTWRQASYLVCNY 207          
BLAST of SCP domain-containing protein vs. Ensembl Xenopus
Match: R3HDML (R3H domain containing-like [Source:NCBI gene;Acc:100492715])

HSP 1 Score: 53.9138 bits (128), Expect = 9.673e-8
Identity = 44/155 (28.39%), Postives = 70/155 (45.16%), Query Frame = 2
Query:  320 SISDVKLILDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYD---------TNTCTSKYCNGYKQLVNARTTEIGCAVSNCKK----GETF--ADYYFCNF 733
            S  D+  +LD  N +R K   PA  M  + W+  LA+ A+  +NQC+       L      +GQ + +  S   +S+     +  +Y+++  Y++           N CT   C  Y Q+V A +  IGCAV+ C      G T+  A Y  CN+
Sbjct:   54 SPRDMSALLDYHNQVRSKVFPPAANMEYMVWDERLAKSAESWANQCKWDHGPNQLMRY---IGQ-NLSVHSGRYRSI--VDLVKGWYDERQHYSFPHPRECNPSCPNKCTGAVCTHYTQMVWASSNRIGCAVNICTNINVWGSTWRQASYLVCNY 202          
BLAST of SCP domain-containing protein vs. Ensembl Mouse
Match: Glipr1l3 (GLI pathogenesis-related 1 like 3 [Source:MGI Symbol;Acc:MGI:3620621])

HSP 1 Score: 74.3294 bits (181), Expect = 9.356e-15
Identity = 57/172 (33.14%), Postives = 86/172 (50.00%), Query Frame = 2
Query:  314 TSSISDVKLI---LDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSE----KQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF-ADYYFCNFYPP--IYQHRPYEKGETKCDSC 793
              SI D K I   L++ N +R K   PA  M+++ W+ +LA+ A+  + +C+LG    N CTS      + Y  +       +   +    +  +YN+ + YN+  NTC SK C  Y Q+  A+T +IGCAVSNC     + A  + CN+ P       RPY KG+  C  C
Sbjct:   33 VPSILDPKFIDAFLNIHNELRRKVQPPAADMNQVIWDQKLAKLAKAWTRECKLGH---NPCTSKQYGCLLDYDFIGENIYLGEIETQPEDVVNNWYNENTDYNFVDNTC-SKICRNYTQVFWAKTFKIGCAVSNCPNLTRYSAGLFVCNYSPTGNFLDFRPYRKGDP-CSMC 199          
BLAST of SCP domain-containing protein vs. Ensembl Mouse
Match: Glipr1l1 (GLI pathogenesis-related 1 like 1 [Source:MGI Symbol;Acc:MGI:1916536])

HSP 1 Score: 72.7886 bits (177), Expect = 2.851e-14
Identity = 54/177 (30.51%), Postives = 92/177 (51.98%), Query Frame = 2
Query:  314 TSSISDVKLI---LDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASP--------MGQ-ISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF-ADYYFCNFYPP--IYQHRPYEKGETKCDSC 793
              +I+D K I   L++ N +R K   PA  M+++ W+ +LA+ A+  + +C+L     N C             +G+ I    + ++ + +     ++ +YN+   +N+D NTC S+ C  Y Q+V A+T +IGCAVSNC   + F A  + CN+ P       RPY +G++ C  C
Sbjct:   33 VPTITDPKFIDAFLNIHNELRRKVQPPAADMNQLFWDQQLAKLAKAWTRECKLAH---NPCIKQRYECLEDYDFIGENIYLGRIETQPEDV-----VINWYNESKYFNFDFNTC-SEMCGHYTQVVWAKTVKIGCAVSNCPNLKGFSAGLFVCNYSPAGNFIGFRPYTRGDS-CSMC 199          
BLAST of SCP domain-containing protein vs. Ensembl Mouse
Match: Pi16 (peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:1921366])

HSP 1 Score: 59.6918 bits (143), Expect = 8.668e-10
Identity = 49/200 (24.50%), Postives = 91/200 (45.50%), Query Frame = 2
Query:  302 VAAPTSSISD--VKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTC-TSKYCNGYKQLVNARTTEIGCAVSNCK--KGETFADYYF--CNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDSQSSD 868
               PT+++++   + ++DL N  R  +  PA  M ++RW+ ELA  A+  + +C  G +           G+      +   + +     +  ++ +   YN+ T TC  ++ C  Y Q+V ++T  IGC    C+  +G   A+ +   CN+ PP  +   +PY++G T C  C        Y C   LC   + + +D
Sbjct:   23 ATGPTTALTEDEKQTMVDLHNQYRAQVSPPASDMLQMRWDDELAAFAKAYAQKCVWGHNKER--------GRRGENLFAITDEGMDVPLAVGNWHEEHEYYNFSTATCDPNQMCGHYTQVVWSKTERIGCGSHFCETLQGVEEANIHLLVCNYEPPGNVKGRKPYQEG-TPCSQC-----PLGYSCENSLCGAENPEKAD 208          
BLAST of SCP domain-containing protein vs. Ensembl Mouse
Match: Pi16 (peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:1921366])

HSP 1 Score: 60.077 bits (144), Expect = 9.195e-10
Identity = 49/192 (25.52%), Postives = 88/192 (45.83%), Query Frame = 2
Query:  302 VAAPTSSISD--VKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTC-TSKYCNGYKQLVNARTTEIGCAVSNCK--KGETFADYYF--CNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCE 844
               PT+++++   + ++DL N  R  +  PA  M ++RW+ ELA  A+  + +C  G +           G+      +   + +     +  ++ +   YN+ T TC  ++ C  Y Q+V ++T  IGC    C+  +G   A+ +   CN+ PP  +   +PY++G T C  C        Y C   LCE
Sbjct:   23 ATGPTTALTEDEKQTMVDLHNQYRAQVSPPASDMLQMRWDDELAAFAKAYAQKCVWGHNKER--------GRRGENLFAITDEGMDVPLAVGNWHEEHEYYNFSTATCDPNQMCGHYTQVVWSKTERIGCGSHFCETLQGVEEANIHLLVCNYEPPGNVKGRKPYQEG-TPCSQC-----PLGYSCENSLCE 200          
BLAST of SCP domain-containing protein vs. Ensembl Mouse
Match: Pi16 (peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:1921366])

HSP 1 Score: 60.077 bits (144), Expect = 9.195e-10
Identity = 49/192 (25.52%), Postives = 88/192 (45.83%), Query Frame = 2
Query:  302 VAAPTSSISD--VKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTC-TSKYCNGYKQLVNARTTEIGCAVSNCK--KGETFADYYF--CNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCE 844
               PT+++++   + ++DL N  R  +  PA  M ++RW+ ELA  A+  + +C  G +           G+      +   + +     +  ++ +   YN+ T TC  ++ C  Y Q+V ++T  IGC    C+  +G   A+ +   CN+ PP  +   +PY++G T C  C        Y C   LCE
Sbjct:   23 ATGPTTALTEDEKQTMVDLHNQYRAQVSPPASDMLQMRWDDELAAFAKAYAQKCVWGHNKER--------GRRGENLFAITDEGMDVPLAVGNWHEEHEYYNFSTATCDPNQMCGHYTQVVWSKTERIGCGSHFCETLQGVEEANIHLLVCNYEPPGNVKGRKPYQEG-TPCSQC-----PLGYSCENSLCE 200          
BLAST of SCP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9DAG6|GPRL1_MOUSE (GLIPR1-like protein 1 OS=Mus musculus OX=10090 GN=Glipr1l1 PE=1 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.994e-13
Identity = 54/177 (30.51%), Postives = 92/177 (51.98%), Query Frame = 2
Query:  314 TSSISDVKLI---LDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASP--------MGQ-ISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF-ADYYFCNFYPP--IYQHRPYEKGETKCDSC 793
              +I+D K I   L++ N +R K   PA  M+++ W+ +LA+ A+  + +C+L     N C             +G+ I    + ++ + +     ++ +YN+   +N+D NTC S+ C  Y Q+V A+T +IGCAVSNC   + F A  + CN+ P       RPY +G++ C  C
Sbjct:   33 VPTITDPKFIDAFLNIHNELRRKVQPPAADMNQLFWDQQLAKLAKAWTRECKLAH---NPCIKQRYECLEDYDFIGENIYLGRIETQPEDV-----VINWYNESKYFNFDFNTC-SEMCGHYTQVVWAKTVKIGCAVSNCPNLKGFSAGLFVCNYSPAGNFIGFRPYTRGDS-CSMC 199          
BLAST of SCP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q58D34|PI16_BOVIN (Peptidase inhibitor 16 OS=Bos taurus OX=9913 GN=PI16 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.832e-10
Identity = 50/180 (27.78%), Postives = 81/180 (45.00%), Query Frame = 2
Query:  335 KLILDLLNNI---RMKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTS-KYCNGYKQLVNARTTEIGCAVSNCKK----GETFADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCE 844
            K ++  L+N+   ++  PA  M ++RW+ ELA  A+  + QC  G +           G+      +   + L     + E+++++  YN    +C + + C  Y Q+V A+T  IGC    C+K     ET      CN+ PP  +   RPY++G T C  C        Y C   LCE
Sbjct:   33 KHVMVELHNLYRTQVSPPATNMLQMRWDEELAAFAKAYAQQCVWGHNKER--------GRRGENLFAITGEGLDVPLAMEEWHHEREHYNLSAISCAAGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIHLLVCNYEPPGNVKGQRPYQEG-TPCSQC-----PLGYHCKNSLCE 198          
BLAST of SCP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6UXB8|PI16_HUMAN (Peptidase inhibitor 16 OS=Homo sapiens OX=9606 GN=PI16 PE=1 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 5.058e-10
Identity = 53/194 (27.32%), Postives = 86/194 (44.33%), Query Frame = 2
Query:  308 APTSSISD--VKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCT-SKYCNGYKQLVNARTTEIGCAVSNCKK----GETFADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDS 856
             P  +++D   +L+++L N  R  +   A  M  +RW+ ELA  A+  + QC  G +           G+      +   + +     + E+++++  YN    TC+  + C  Y Q+V A+T  IGC    C+K     ET  +   CN+ PP  +   RPY++G T C  C        Y C   LCE   S
Sbjct:   23 GPVGALTDEEKRLMVELHNLYRAQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKER--------GRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEG-TPCSQCPS-----GYHCKNSLCEPIGS 202          
BLAST of SCP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6UWM5|GPRL1_HUMAN (GLIPR1-like protein 1 OS=Homo sapiens OX=9606 GN=GLIPR1L1 PE=1 SV=2)

HSP 1 Score: 60.077 bits (144), Expect = 4.107e-9
Identity = 58/198 (29.29%), Postives = 89/198 (44.95%), Query Frame = 2
Query:  296 CQVAAPTS---SISDVKLI---LDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGS----DNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKK-GETFADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCE 844
            C VA  +S   SI+D   I   ++  N  R K   PA  M  + W+  LA+ A+  +NQC+       D +  C +A     +         +S      I  +YN+   Y++D+ +C S+ C  Y QLV A +  +GCAV+ C   G      + CN+ P        PY +GE+ C  C K +     +C K+LC 
Sbjct:   16 CLVATTSSKIPSITDPHFIDNCIEAHNEWRGKVNPPAADMKYMIWDKGLAKMAKAWANQCKFEHNDCLDKSYKCYAA--FEYVGENIWLGGIKSFTPRHAITAWYNETQFYDFDSLSC-SRVCGHYTQLVWANSFYVGCAVAMCPNLGGASTAIFVCNYGPAGNFANMPPYVRGES-CSLCSKEE-----KCVKNLCR 204          
BLAST of SCP domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9ET66|PI16_MOUSE (Peptidase inhibitor 16 OS=Mus musculus OX=10090 GN=Pi16 PE=1 SV=2)

HSP 1 Score: 59.3066 bits (142), Expect = 2.961e-8
Identity = 49/190 (25.79%), Postives = 88/190 (46.32%), Query Frame = 2
Query:  308 APTSSISD--VKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTC-TSKYCNGYKQLVNARTTEIGCAVSNCK--KGETFADYYF--CNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCE 844
             PT+++++   + ++DL N  R  +  PA  M ++RW+ ELA  A+  + +C  G +           G+      +   + +     +  ++ +   YN+ T TC  ++ C  Y Q+V ++T  IGC    C+  +G   A+ +   CN+ PP  +   +PY++G T C  C        Y C   LCE
Sbjct:   25 GPTTALTEDEKQTMVDLHNQYRAQVSPPASDMLQMRWDDELAAFAKAYAQKCVWGHNKER--------GRRGENLFAITDEGMDVPLAVGNWHEEHEYYNFSTATCDPNQMCGHYTQVVWSKTERIGCGSHFCETLQGVEEANIHLLVCNYEPPGNVKGRKPYQEG-TPCSQC-----PLGYSCENSLCE 200          
BLAST of SCP domain-containing protein vs. TrEMBL
Match: T1FCL9 (SCP domain-containing protein OS=Helobdella robusta OX=6412 GN=20206568 PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 2.429e-15
Identity = 53/151 (35.10%), Postives = 79/151 (52.32%), Query Frame = 2
Query:  308 APTSSISDVKLILDLLNNIRMKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTS-KYCNGYKQLVNARTTEIGCAVSNCKKGETFADY-----YFCNFYPP 742
            A   S SDVK I+D  N +R KE + +M  I+W+++LA  A   +  C           +   +GQ  YA+ +S   ++ +   I  +Y++K  Y+YD+N+C S K C  Y Q+V A TTE+GCAV  C KG     +       CN+ PP
Sbjct:   19 AEVPSASDVKKIVDKHNLLRKKEGSSKMLPIKWSSKLANYASAWAQNCAFEHGQPAEAQNEDDIGQNLYASSTS---NVDYESAIQNWYDEKPDYSYDSNSCRSGKMCGHYTQVVWATTTEVGCAVQMCNKGIEGLPWPRGHIIVCNYLPP 166          
BLAST of SCP domain-containing protein vs. TrEMBL
Match: A0A3P8YYP9 (SCP domain-containing protein OS=Esox lucius OX=8010 PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 2.302e-12
Identity = 53/158 (33.54%), Postives = 83/158 (52.53%), Query Frame = 2
Query:  335 KLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTN-TCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETFADYYFCNFYPP--IYQHRPYEKGETKCDSC 793
            K I+DL N++R  ++ PA  M ++ WN E A  AQ  +NQC +     +   + S  G+  +  MS+ ++   WT  I  +Y++ + YNYD+N +   K    Y Q+V A + EIGCAV+ C     +  YY C + PP      +PY  G ++C  C
Sbjct:   32 KEIVDLHNDLRRGVQPPARNMLQMSWNNEAAINAQKWANQCTMSHSPADQRRTTSGCGENLF--MSTNER--PWTSAIQSWYSEVNNYNYDSNGSINGKVVGHYTQVVWATSKEIGCAVAKCPN-SVWKFYYVCQYCPPGNYVGRKPYNTG-SRCADC 183          
BLAST of SCP domain-containing protein vs. TrEMBL
Match: A0A183AIH1 (SCP domain-containing protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS6756 PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.177e-11
Identity = 45/146 (30.82%), Postives = 73/146 (50.00%), Query Frame = 2
Query:  341 ILDLLNNIRMK---------EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNT--NLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF--ADYYFCNFYP 739
            IL L N  R +          PA  M+ +RWN  LA +AQ +++QCR+G D+           +GQ ++A +   K S+   + +  ++ +   Y++ +  C S  C  Y QL+ A+TT++GC V +C  G TF       CN+ P
Sbjct:   28 ILQLHNEYRQQIRSCSATGQPPAKSMALLRWNENLAMKAQRLADQCRVGHDSAEERQTDPWQWVGQ-NWAGVQDVKTSVPSLKAVKMWFEEYVNYDFGSGQCRSGMCGHYTQLIWAKTTDVGCGVRDCGSGATFPYGLSIVCNYGP 172          
BLAST of SCP domain-containing protein vs. TrEMBL
Match: A0A0A0A5H2 (Cysteine-rich secretory protein 2 (Fragment) OS=Charadrius vociferus OX=50402 GN=N301_07789 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.446e-11
Identity = 54/170 (31.76%), Postives = 81/170 (47.65%), Query Frame = 2
Query:  305 AAPTSSISDVKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNG-YKQLVNARTTEIGCAVSNCKKGETFADYYFCNFYPP----IYQHRPYEKGETKCDSC 793
            A  TS I   KLI+D  N +R  +K  A  M K+ W+   A  AQ  +NQC L     N+ T+  P G+  + + +       W+  I  +YN++  + Y T   T     G Y QL+   + +IGCAV+ C + + F  +Y C + PP    +    PY+ G  KC  C
Sbjct:    1 ALSTSKIDQQKLIVDKHNTLRRGVKPTASNMLKMEWDPAAANNAQKWANQCTLSHSPANMRTTTVPCGENLFMSTA----PFSWSDAIQAWYNEEKDFEYGTGAKTQGAVIGHYTQLIWYNSYQIGCAVAYCPRNQ-FNYFYVCQYCPPGNNAMQIATPYKSGP-KCADC 164          
BLAST of SCP domain-containing protein vs. TrEMBL
Match: A0A1W2P7V2 (GLI pathogenesis-related 1-like 3 OS=Mus musculus OX=10090 GN=Glipr1l3 PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.940e-11
Identity = 57/172 (33.14%), Postives = 86/172 (50.00%), Query Frame = 2
Query:  314 TSSISDVKLI---LDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSE----KQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF-ADYYFCNFYPP--IYQHRPYEKGETKCDSC 793
              SI D K I   L++ N +R K   PA  M+++ W+ +LA+ A+  + +C+LG    N CTS      + Y  +       +   +    +  +YN+ + YN+  NTC SK C  Y Q+  A+T +IGCAVSNC     + A  + CN+ P       RPY KG+  C  C
Sbjct:   33 VPSILDPKFIDAFLNIHNELRRKVQPPAADMNQVIWDQKLAKLAKAWTRECKLGH---NPCTSKQYGCLLDYDFIGENIYLGEIETQPEDVVNNWYNENTDYNFVDNTC-SKICRNYTQVFWAKTFKIGCAVSNCPNLTRYSAGLFVCNYSPTGNFLDFRPYRKGDP-CSMC 199          
BLAST of SCP domain-containing protein vs. Ensembl Cavefish
Match: crispld1b (cysteine rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:103033853])

HSP 1 Score: 61.2326 bits (147), Expect = 6.933e-10
Identity = 54/193 (27.98%), Postives = 86/193 (44.56%), Query Frame = 2
Query:  320 SISDVKLILDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYD---------TNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF------ADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLC 841
            S SD++LILDL N +R +   PA  M  + W+TEL + A+  ++ C       +L      +GQ   A    ++     T  +  +Y++   ++Y             C+   C  Y QLV A + +IGCA++ C     +      A Y  CN+ PP   + H PY+ G   C +C     +Y   C  +LC
Sbjct:   58 SASDMQLILDLHNKLRGQVYPPASNMEYMVWDTELERSAEDWAHTCLWEHGPPHLLRQ---IGQNLGAHWGRDRPP---TFHVQAWYDEVQYFSYPYPQECDPYCPYRCSGPVCTHYTQLVWATSNKIGCAINVCYNMNVWGMIWAKAVYLVCNYSPPGNWWGHAPYKHGH-PCSAC---PASYGGGCRDNLC 240          
BLAST of SCP domain-containing protein vs. Ensembl Cavefish
Match: crispld1b (cysteine rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:103033853])

HSP 1 Score: 60.8474 bits (146), Expect = 8.906e-10
Identity = 54/194 (27.84%), Postives = 86/194 (44.33%), Query Frame = 2
Query:  320 SISDVKLILDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYD---------TNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF------ADYYFCNFYPP---IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLC 841
            S SD++LILDL N +R +   PA  M  + W+TEL + A+  ++ C       +L      +GQ   A    ++     T  +  +Y++   ++Y             C+   C  Y QLV A + +IGCA++ C     +      A Y  CN+ PP    + H PY+ G   C +C     +Y   C  +LC
Sbjct:   58 SASDMQLILDLHNKLRGQVYPPASNMEYMVWDTELERSAEDWAHTCLWEHGPPHLLRQ---IGQNLGAHWGRDRPP---TFHVQAWYDEVQYFSYPYPQECDPYCPYRCSGPVCTHYTQLVWATSNKIGCAINVCYNMNVWGMIWAKAVYLVCNYSPPPGNWWGHAPYKHGH-PCSAC---PASYGGGCRDNLC 241          
BLAST of SCP domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000031557.1 (peptidase inhibitor 16 [Source:NCBI gene;Acc:103029846])

HSP 1 Score: 58.9214 bits (141), Expect = 1.268e-9
Identity = 53/186 (28.49%), Postives = 87/186 (46.77%), Query Frame = 2
Query:  329 DVKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQC--RLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCT-SKYCNGYKQLVNARTTEIGCAVSNCKKGETF----ADYYFCNFYP--PIYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICD 853
            D + I++L N+ R  ++ PA  M  ++W+ ELA  A+  + +C      D+ N+      MG+  + T       L   + + +++ +   Y+YD NTC  ++ C  Y Q+V A+T E+GC    C+  E      A    CN+ P       +PY+ GE +C  C   DD  L  C    C+  D
Sbjct:   29 DQQRIVNLHNHHRSTVEPPAADMIHMKWSGELALIAKEYATKCIWEHNPDSQNI------MGENLFITTGP----LDIEKALSDWFMEHKHYSYDNNTCAENEMCGHYTQMVWAKTKEVGCGSHLCETVEALGFKNATLLVCNYLPLGNYMGQKPYQVGE-QCSKC--PDD--LKNCVDQACDSTD 199          
BLAST of SCP domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000036030.1 (peptidase inhibitor 16 [Source:NCBI gene;Acc:103029846])

HSP 1 Score: 58.9214 bits (141), Expect = 1.429e-9
Identity = 53/184 (28.80%), Postives = 88/184 (47.83%), Query Frame = 2
Query:  329 DVKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCT-SKYCNGYKQLVNARTTEIGCAVSNCKKGETF----ADYYFCNFYP--PIYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICD 853
            D + I++L N+ R  ++ PA  M  ++W+ ELA  A+  + +C +   N +   S + MG+  + T       L   + + +++ +   Y+YD NTC  ++ C  Y Q+V A+T E+GC    C+  E      A    CN+ P       +PY+ GE +C  C   DD  L  C    C+  D
Sbjct:   29 DQQRIVNLHNHHRSTVEPPAADMIHMKWSGELALIAKEYATKC-IWEHNPD---SQNIMGENLFITTGP----LDIEKALSDWFMEHKHYSYDNNTCAENEMCGHYTQMVWAKTKEVGCGSHLCETVEALGFKNATLLVCNYLPLGNYMGQKPYQVGE-QCSKC--PDD--LKNCVDQACDSTD 199          
BLAST of SCP domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000029824.1 (peptidase inhibitor 16 [Source:NCBI gene;Acc:103029846])

HSP 1 Score: 58.9214 bits (141), Expect = 1.678e-9
Identity = 53/186 (28.49%), Postives = 87/186 (46.77%), Query Frame = 2
Query:  329 DVKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQC--RLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCT-SKYCNGYKQLVNARTTEIGCAVSNCKKGETF----ADYYFCNFYP--PIYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICD 853
            D + I++L N+ R  ++ PA  M  ++W+ ELA  A+  + +C      D+ N+      MG+  + T       L   + + +++ +   Y+YD NTC  ++ C  Y Q+V A+T E+GC    C+  E      A    CN+ P       +PY+ GE +C  C   DD  L  C    C+  D
Sbjct:   29 DQQRIVNLHNHHRSTVEPPAADMIHMKWSGELALIAKEYATKCIWEHNPDSQNI------MGENLFITTGP----LDIEKALSDWFMEHKHYSYDNNTCAENEMCGHYTQMVWAKTKEVGCGSHLCETVEALGFKNATLLVCNYLPLGNYMGQKPYQVGE-QCSKC--PDD--LKNCVDQACDSTD 199          
BLAST of SCP domain-containing protein vs. Ensembl Sea Lamprey
Match: CRISP3 (cysteine rich secretory protein 3 [Source:HGNC Symbol;Acc:HGNC:16904])

HSP 1 Score: 58.151 bits (139), Expect = 5.817e-10
Identity = 53/179 (29.61%), Postives = 80/179 (44.69%), Query Frame = 2
Query:  338 LILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLG-SDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQ--KSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETFADYYFCNFYPPIYQ-----HRPYEKGETKCDSCYKLDDNYLYRCTKDLCE 844
             I+DL N++R  +   A  M ++ W  E    A   +N C  G S +    T+    G+  +  MSS      W   I ++YN+    G+ YD  + T+     Y QLV   + ++GCAV+ C K    A Y +   Y P+       +RPY+ G T C SC K        C  +LC+
Sbjct:   46 FIVDLHNDLRRNVTPSAGNMLQMMWKKEAYLNALSWANNCTFGHSPDARRKTTQWTCGENIF--MSSAP--FTWNYAIRDWYNEVISPGFQYDVGSTTTGAVGHYTQLVWYNSFQLGCAVNYCPKNPGVAKYLYVCHYCPMGNLNTRINRPYDLG-TSCASCPK-------NCVNNLCD 212          
BLAST of SCP domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000374.1 (pep scaffold:Pmarinus_7.0:GL476650:75849:87118:-1 gene:ENSPMAG00000000334.1 transcript:ENSPMAT00000000374.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.9062 bits (115), Expect = 1.134e-6
Identity = 46/188 (24.47%), Postives = 74/188 (39.36%), Query Frame = 2
Query:  335 KLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYD---------TNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGET------FADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLC 841
            + + +  N +R  +   A  M  + W+ ELA  A+  + QC+ G   ++   +   +GQ     + SE+        +  +++++  Y Y           +TC    C  Y QLV A +  +GCAV  C           +A Y  CN+ P        PY +G T C  C      Y   C   LC
Sbjct:    7 RALTEYHNRLRASVHPQAADMEHMSWDWELAGMAEAWAKQCKWGHGPSDQLDA---IGQNLAVYVGSER---PLAYHVQAWFDEEEWYQYPEEKECSPECPHTCRGPICTHYTQLVWAESRRVGCAVHTCLDISVWDAVWPYALYLVCNYSPKGNWIGEAPYRQG-TPCSRC---PPRYGGSCRGGLC 184          
BLAST of SCP domain-containing protein vs. Ensembl Medaka
Match: crispld1b (cysteine-rich secretory protein LCCL domain-containing 1 [Source:NCBI gene;Acc:101169145])

HSP 1 Score: 66.2402 bits (160), Expect = 1.517e-11
Identity = 54/193 (27.98%), Postives = 86/193 (44.56%), Query Frame = 2
Query:  320 SISDVKLILDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTN---------TCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF------ADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLC 841
            S  D+ LI+DL N +R +   PA  M  + W+ EL + A+Y ++ CR      ++ T    +GQ        ++     T  +  +Y++   Y+Y  +          C+   C  Y QLV A +  IGCA++ C     +      A Y  CN+ PP   + H PY+ G T C +C     +Y   C  +LC
Sbjct:   62 SQEDMHLIVDLHNKLRGQVYPPASNMEYMMWDYELERSAEYWAHMCRWEHGPNHMLTQ---IGQNLGVHWGRDRPP---TYHVQAWYDEVRHYSYPYSQECNPHCPLRCSGPVCTHYTQLVWATSNRIGCAINVCYNMNVWGMIWAKAVYLVCNYSPPGNWWGHAPYKYG-TPCSAC---PASYAGGCRDNLC 244          
BLAST of SCP domain-containing protein vs. Ensembl Medaka
Match: crispld1b (cysteine-rich secretory protein LCCL domain-containing 1 [Source:NCBI gene;Acc:101169145])

HSP 1 Score: 66.2402 bits (160), Expect = 2.072e-11
Identity = 54/193 (27.98%), Postives = 86/193 (44.56%), Query Frame = 2
Query:  320 SISDVKLILDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTN---------TCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF------ADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLC 841
            S  D+ LI+DL N +R +   PA  M  + W+ EL + A+Y ++ CR      ++ T    +GQ        ++     T  +  +Y++   Y+Y  +          C+   C  Y QLV A +  IGCA++ C     +      A Y  CN+ PP   + H PY+ G T C +C     +Y   C  +LC
Sbjct:  113 SQEDMHLIVDLHNKLRGQVYPPASNMEYMMWDYELERSAEYWAHMCRWEHGPNHMLTQ---IGQNLGVHWGRDRPP---TYHVQAWYDEVRHYSYPYSQECNPHCPLRCSGPVCTHYTQLVWATSNRIGCAINVCYNMNVWGMIWAKAVYLVCNYSPPGNWWGHAPYKYG-TPCSAC---PASYAGGCRDNLC 295          
BLAST of SCP domain-containing protein vs. Ensembl Medaka
Match: crispld1a (cysteine rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:101172850])

HSP 1 Score: 60.077 bits (144), Expect = 1.612e-9
Identity = 52/191 (27.23%), Postives = 83/191 (43.46%), Query Frame = 2
Query:  326 SDVKLILDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNT---------CTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF------ADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLC 841
            SD +LILDL N +R +   PA  M  + W+TEL + A+  +  C       +L      + QI     +   +    T  +  +Y++   Y++             C+   C  Y QLV A ++ IGCA++ C   + +      A Y  CN+ P    + H PY+ G T C +C     +Y   C  +LC
Sbjct:   59 SDAQLILDLHNKLRGQVYPPASNMEYMMWDTELERSAEEWAETCLWEHGPASL------LPQIGQNLGAHWGRYQPPTFHVQAWYDEVKDYSFPYPQECNPYCPFRCSGPVCTHYTQLVWATSSRIGCAINLCHNMDVWGQIWAKAVYLVCNYSPKGNWWGHAPYKHG-TPCSAC---PPSYGGGCRDNLC 239          
BLAST of SCP domain-containing protein vs. Ensembl Medaka
Match: crispld1a (cysteine rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:101172850])

HSP 1 Score: 60.077 bits (144), Expect = 1.730e-9
Identity = 52/191 (27.23%), Postives = 83/191 (43.46%), Query Frame = 2
Query:  326 SDVKLILDLLNNIRMK--EPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNT---------CTSKYCNGYKQLVNARTTEIGCAVSNCKKGETF------ADYYFCNFYPP--IYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLC 841
            SD +LILDL N +R +   PA  M  + W+TEL + A+  +  C       +L      + QI     +   +    T  +  +Y++   Y++             C+   C  Y QLV A ++ IGCA++ C   + +      A Y  CN+ P    + H PY+ G T C +C     +Y   C  +LC
Sbjct:   87 SDAQLILDLHNKLRGQVYPPASNMEYMMWDTELERSAEEWAETCLWEHGPASL------LPQIGQNLGAHWGRYQPPTFHVQAWYDEVKDYSFPYPQECNPYCPFRCSGPVCTHYTQLVWATSSRIGCAINLCHNMDVWGQIWAKAVYLVCNYSPKGNWWGHAPYKHG-TPCSAC---PPSYGGGCRDNLC 267          
BLAST of SCP domain-containing protein vs. Ensembl Medaka
Match: CLEC18A (C-type lectin domain family 18 member A [Source:HGNC Symbol;Acc:HGNC:30388])

HSP 1 Score: 51.6026 bits (122), Expect = 2.796e-7
Identity = 35/149 (23.49%), Postives = 69/149 (46.31%), Query Frame = 2
Query:  320 SISDVKLILDLLNNIR--MKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQ-ISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCT-SKYCNGYKQLVNARTTEIGCAVSNCKKGETFADYYFCNFYPPIYQH 754
             I    +I+ L N +R  ++  A  M K+ W+  +A  A+  + +C  G+D   L     P  + I + T  S  +   ++  I  ++ + + + + +  C  +  C  Y QLV A ++ +GCA   C +     + + C +YP ++ H
Sbjct:    2 GIKQRSVIVALHNRLRSRVRPMAANMQKMEWDGRMAILAEKQAMRC--GAD---LSPQNGPFSRHIGWNTHLSTHRIASFSDIIHAWFEEGNDFFFLSGKCRENAACQHYTQLVWATSSHVGCASQLCLRDGDIWEIFVCAYYPGLWDH 145          
BLAST of SCP domain-containing protein vs. Planmine SMEST
Match: SMESG000004631.1 (SMESG000004631.1)

HSP 1 Score: 648.277 bits (1671), Expect = 0.000e+0
Identity = 311/313 (99.36%), Postives = 312/313 (99.68%), Query Frame = 2
Query:   71 MIIFQVAFLFAVACSVAQCQYAYRPKTENGEFCMIPFEYQGKIFHDCTTEGDSKAWCRPASDKWGYCSNTSDFSKCQVAAPTSSISDVKLILDLLNNIRMKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETFADYYFCNFYPPIYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDSQSSDCQGKEVLKLAIEAGLCEDLEPGFCELNASVCPNIDLLPDEYKVMMTS 1009
            MI FQVAFLFA+ACSVAQCQYAYRPKTENGEFCMIPFEYQGKIFHDCTTEGDSKAWCRPASDKWGYCSNTSDFSKCQVAAPTSSISDVKLILDLLNNIRMKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETFADYYFCNFYPPIYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDSQSSDCQGKEVLKLAIEAGLCEDLEPGFCELNASVCPNIDLLPDEYKVMMTS
Sbjct:    1 MINFQVAFLFALACSVAQCQYAYRPKTENGEFCMIPFEYQGKIFHDCTTEGDSKAWCRPASDKWGYCSNTSDFSKCQVAAPTSSISDVKLILDLLNNIRMKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETFADYYFCNFYPPIYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDSQSSDCQGKEVLKLAIEAGLCEDLEPGFCELNASVCPNIDLLPDEYKVMMTS 313          
BLAST of SCP domain-containing protein vs. Planmine SMEST
Match: SMESG000004629.1 (SMESG000004629.1)

HSP 1 Score: 431.795 bits (1109), Expect = 6.531e-152
Identity = 199/314 (63.38%), Postives = 251/314 (79.94%), Query Frame = 2
Query:   71 MIIFQVAFLFAVACSVAQCQYAYRPKTENGEFCMIPFEYQGKIFHDCTTEGDSKAWCRPASDKWGYCSNTSDFSKCQVAAPTSSISDVKLILDLLNNIRMKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETFADYYFCNFYPPIYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDSQSSDCQGKEVLKLAIEA-GLCEDLEPGFCELNASVCPNIDLLPDEYKVMMTS 1009
            MI F+  FL  + CS+   +  YRPKTENGEFC IPFE   K++HDCT E DSK WC+ + D   YC+N +DFS C V  PT+S+++VK +L+L+NNIR KEPA+RM+KIRWNTELA RAQYMS+QC++GSDNTNLC+S SPMGQIS+ +MS++ Q  KW+QFILEFYNQKS YN+DTN C  KYCNGYKQLVN++TT+IGCAV++C     ++DYYFCNFYPP+Y  +PYEKGE  C+SCYKL+DNY YRC   LCEICD+ S+DC+GKE L+  +E+ GLCEDL+  FC+LN SVCPNIDLLP++ K+M+ S
Sbjct:    1 MIEFRNLFLLGI-CSMVFVEALYRPKTENGEFCKIPFEKNDKVYHDCTKEEDSKPWCKNSEDVVSYCANVTDFSNCNVGTPTTSVAEVKSVLELVNNIRTKEPAVRMAKIRWNTELAIRAQYMSDQCQVGSDNTNLCSSDSPMGQISFMSMSTDPQPKKWSQFILEFYNQKSSYNFDTNECNGKYCNGYKQLVNSKTTDIGCAVTSCNLDGVYSDYYFCNFYPPVYMGKPYEKGEGNCESCYKLNDNYSYRCNGKLCEICDAPSNDCKGKEELQKQVESGGLCEDLDSSFCKLNESVCPNIDLLPEDQKLMVQS 313          
BLAST of SCP domain-containing protein vs. Planmine SMEST
Match: SMESG000004629.1 (SMESG000004629.1)

HSP 1 Score: 423.32 bits (1087), Expect = 9.132e-149
Identity = 192/292 (65.75%), Postives = 240/292 (82.19%), Query Frame = 2
Query:  137 YRPKTENGEFCMIPFEYQGKIFHDCTTEGDSKAWCRPASDKWGYCSNTSDFSKCQVAAPTSSISDVKLILDLLNNIRMKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETFADYYFCNFYPPIYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDSQSSDCQGKEVLKLAIEA-GLCEDLEPGFCELNASVCPNIDLLPDEYKVMMTS 1009
            YRPKTENGEFC IPFE   K++HDCT E DSK WC+ + D   YC+N +DFS C V  PT+S+++VK +L+L+NNIR KEPA+RM+KIRWNTELA RAQYMS+QC++GSDNTNLC+S SPMGQIS+ +MS++ Q  KW+QFILEFYNQKS YN+DTN C  KYCNGYKQLVN++TT+IGCAV++C     ++DYYFCNFYPP+Y  +PYEKGE  C+SCYKL+DNY YRC   LCEICD+ S+DC+GKE L+  +E+ GLCEDL+  FC+LN SVCPNIDLLP++ K+M+ S
Sbjct:    8 YRPKTENGEFCKIPFEKNDKVYHDCTKEEDSKPWCKNSEDVVSYCANVTDFSNCNVGTPTTSVAEVKSVLELVNNIRTKEPAVRMAKIRWNTELAIRAQYMSDQCQVGSDNTNLCSSDSPMGQISFMSMSTDPQPKKWSQFILEFYNQKSSYNFDTNECNGKYCNGYKQLVNSKTTDIGCAVTSCNLDGVYSDYYFCNFYPPVYMGKPYEKGEGNCESCYKLNDNYSYRCNGKLCEICDAPSNDCKGKEELQKQVESGGLCEDLDSSFCKLNESVCPNIDLLPEDQKLMVQS 299          
BLAST of SCP domain-containing protein vs. Planmine SMEST
Match: SMESG000004630.1 (SMESG000004630.1)

HSP 1 Score: 305.064 bits (780), Expect = 8.614e-103
Identity = 136/247 (55.06%), Postives = 182/247 (73.68%), Query Frame = 2
Query:  137 YRPKTENGEFCMIPFEYQGKIFHDCTTEGDSKAWCRPASDKWGYCSNTSDFSKCQVAAPTSSISDVKLILDLLNNIRMKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYDTNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETFADYYFCNFYPPIYQHRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDSQSSDCQG 877
            YRPKT+ G+FC IPFE  GK++HDC T+   K WC+ +    G+C+N  DFSKC+V+ P  ++ +V+ IL+++NNIR KEPALRM+KIRWNTELA  AQY+S+QC++ S N NLC+SA PMGQIS+ TMSS  Q  KW QFI + +NQK  YN+D N C SK CNGYKQLVNA+TT+IGC V++C +   + DYY+C+F+ P Y  RPYEKG   C++C  L++ Y+++C  +LCEI DS  S  +G
Sbjct:   22 YRPKTDKGDFCKIPFEKNGKVYHDCGTDRGKKPWCQNSKGSVGHCANVQDFSKCKVSQPKVTVKEVESILEIVNNIRSKEPALRMAKIRWNTELALTAQYLSDQCKIKSSNPNLCSSALPMGQISFMTMSSSPQPKKWNQFIQDVFNQKPSYNFDKNKCKSKNCNGYKQLVNAKTTDIGCGVTSCNRPGEYIDYYYCHFFTPAYLDRPYEKGNKGCNTCNLLNEKYVFKCNNNLCEIVDSPPSKFKG 268          
BLAST of SCP domain-containing protein vs. Planmine SMEST
Match: SMESG000050444.1 (SMESG000050444.1)

HSP 1 Score: 157.147 bits (396), Expect = 3.407e-44
Identity = 106/335 (31.64%), Postives = 163/335 (48.66%), Query Frame = 2
Query:   74 IIFQVAFLFAVACSVAQCQYA-YRPKTENGEFCMIPFEYQGKIFHDCTTEGDSKAWCRPASD--KWGYCS-NTSDFSKCQVAAPTSSISDVKLILDLLNNIRMKEPALRMSKIRWNTELAQRAQYMSNQCRLGSDNTNLCTSASPMGQISYATMSSEKQS--LKWTQFILEFYNQKSGYNYDTNTCT-SKYCNGYKQLVNARTTEIGCAVSNCKKGETFAD-----YYFCNFYPP--IYQHRPYEK--GETKCDSCYKLDDNYLYRCTKD-LC----------------EICDSQSSDCQGK-EVLKLAIEAGLCEDLEPGFCELNASVCPNIDL 976
            I F V  +F     +AQ Q   Y+ +T  G+ C IPF Y+GKI+HDC TEG    WC    +  KW YC  +  ++  CQ+  P  ++ DV+ IL + N  R K PALRM K+ WN+ELAQ AQ  +++C     +  LC    P+GQ  + + + +     L WT  I  +  ++  Y+ DT++C   K C  +  +    T  +GC  + C  GE          Y+CN+Y    +   +P+ K  GE KC  C+  + N  +RC +D LC                E CDS   DC G  ++ K+     +C D +   C++N ++C  + +
Sbjct:   14 ICFIVIAIFHGLVDLAQGQLTIYKAETTTGQRCTIPFVYKGKIYHDCITEGSDSPWCYVDKEFKKWEYCKPHPKEYDGCQIRNPNVTLEDVEEILRVHNKFRSKLPALRMKKLEWNSELAQIAQKAADKCAHVHIDVTLCGDREPVGQNLFYSYNDDDYPGLLSWTDIIGHWSGEEKNYDIDTHSCLPGKLCGHFTAVATDYTNLVGCGRNFC-YGELLGKKRYSIQYYCNYYEAGNVGGQKPFIKSTGE-KCKDCHLFNQNERFRCDEDQLCGKLMDIFIDFHSSFHSETCDSTMKDCMGMVDLKKMEASGNICVDTDIN-CQVNINLCAALKM 345          
The following BLAST results are available for this feature:
BLAST of SCP domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PI161.053e-1027.32peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGN... [more]
PI161.053e-1027.32peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGN... [more]
PI161.053e-1027.32peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGN... [more]
GLIPR1L15.339e-1029.29GLIPR1 like 1 [Source:HGNC Symbol;Acc:HGNC:28392][more]
GLIPR1L18.552e-1029.29GLIPR1 like 1 [Source:HGNC Symbol;Acc:HGNC:28392][more]
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BLAST of SCP domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCP domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG84832.469e-626.80gene:FBgn0038126 transcript:FBtr0082782[more]
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BLAST of SCP domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
crispld1b7.768e-1229.02cysteine-rich secretory protein LCCL domain contai... [more]
CU914487.13.638e-939.77peptidase inhibitor 16-like [Source:NCBI gene;Acc:... [more]
crispld1a1.982e-725.11cysteine-rich secretory protein LCCL domain contai... [more]
crispld1a2.501e-725.12cysteine-rich secretory protein LCCL domain contai... [more]
glipr1a4.592e-623.83GLI pathogenesis-related 1a [Source:ZFIN;Acc:ZDB-G... [more]
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BLAST of SCP domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CRISPLD11.841e-827.23cysteine-rich secretory protein LCCL domain contai... [more]
CRISPLD14.027e-827.23cysteine-rich secretory protein LCCL domain contai... [more]
CRISPLD15.878e-827.23cysteine-rich secretory protein LCCL domain contai... [more]
R3HDML5.917e-828.39R3H domain containing-like [Source:NCBI gene;Acc:1... [more]
R3HDML9.673e-828.39R3H domain containing-like [Source:NCBI gene;Acc:1... [more]
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BLAST of SCP domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Glipr1l39.356e-1533.14GLI pathogenesis-related 1 like 3 [Source:MGI Symb... [more]
Glipr1l12.851e-1430.51GLI pathogenesis-related 1 like 1 [Source:MGI Symb... [more]
Pi168.668e-1024.50peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:... [more]
Pi169.195e-1025.52peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:... [more]
Pi169.195e-1025.52peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:... [more]
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BLAST of SCP domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9DAG6|GPRL1_MOUSE1.994e-1330.51GLIPR1-like protein 1 OS=Mus musculus OX=10090 GN=... [more]
sp|Q58D34|PI16_BOVIN2.832e-1027.78Peptidase inhibitor 16 OS=Bos taurus OX=9913 GN=PI... [more]
sp|Q6UXB8|PI16_HUMAN5.058e-1027.32Peptidase inhibitor 16 OS=Homo sapiens OX=9606 GN=... [more]
sp|Q6UWM5|GPRL1_HUMAN4.107e-929.29GLIPR1-like protein 1 OS=Homo sapiens OX=9606 GN=G... [more]
sp|Q9ET66|PI16_MOUSE2.961e-825.79Peptidase inhibitor 16 OS=Mus musculus OX=10090 GN... [more]
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BLAST of SCP domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1FCL92.429e-1535.10SCP domain-containing protein OS=Helobdella robust... [more]
A0A3P8YYP92.302e-1233.54SCP domain-containing protein OS=Esox lucius OX=80... [more]
A0A183AIH11.177e-1130.82SCP domain-containing protein OS=Echinostoma capro... [more]
A0A0A0A5H21.446e-1131.76Cysteine-rich secretory protein 2 (Fragment) OS=Ch... [more]
A0A1W2P7V21.940e-1133.14GLI pathogenesis-related 1-like 3 OS=Mus musculus ... [more]
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BLAST of SCP domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
crispld1b6.933e-1027.98cysteine rich secretory protein LCCL domain contai... [more]
crispld1b8.906e-1027.84cysteine rich secretory protein LCCL domain contai... [more]
ENSAMXT00000031557.11.268e-928.49peptidase inhibitor 16 [Source:NCBI gene;Acc:10302... [more]
ENSAMXT00000036030.11.429e-928.80peptidase inhibitor 16 [Source:NCBI gene;Acc:10302... [more]
ENSAMXT00000029824.11.678e-928.49peptidase inhibitor 16 [Source:NCBI gene;Acc:10302... [more]
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BLAST of SCP domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CRISP35.817e-1029.61cysteine rich secretory protein 3 [Source:HGNC Sym... [more]
ENSPMAT00000000374.11.134e-624.47pep scaffold:Pmarinus_7.0:GL476650:75849:87118:-1 ... [more]
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BLAST of SCP domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCP domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SCP domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
crispld1b1.517e-1127.98cysteine-rich secretory protein LCCL domain-contai... [more]
crispld1b2.072e-1127.98cysteine-rich secretory protein LCCL domain-contai... [more]
crispld1a1.612e-927.23cysteine rich secretory protein LCCL domain contai... [more]
crispld1a1.730e-927.23cysteine rich secretory protein LCCL domain contai... [more]
CLEC18A2.796e-723.49C-type lectin domain family 18 member A [Source:HG... [more]
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BLAST of SCP domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000004631.10.000e+099.36SMESG000004631.1[more]
SMESG000004629.16.531e-15263.38SMESG000004629.1[more]
SMESG000004629.19.132e-14965.75SMESG000004629.1[more]
SMESG000004630.18.614e-10355.06SMESG000004630.1[more]
SMESG000050444.13.407e-4431.64SMESG000050444.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034978 ID=SMED30034978|Name=SCP domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1151bp
TAATAATAATAATATAAATATTAAAAATAGAAATGATAATATTAACAATA
ACAGTTCTTTCCTAGATAGGATGATAATTTTCCAAGTTGCTTTTTTATTT
GCCGTAGCTTGTTCAGTGGCGCAATGTCAGTACGCTTATAGACCAAAAAC
TGAAAATGGTGAATTCTGCATGATTCCATTCGAGTATCAAGGCAAAATCT
TTCATGACTGCACAACTGAAGGAGATTCCAAAGCATGGTGTCGGCCTGCA
TCAGATAAATGGGGCTATTGTTCCAATACCTCAGATTTTAGTAAGTGTCA
AGTTGCCGCACCGACATCGTCAATATCAGATGTGAAATTAATTTTGGATT
TATTGAACAACATTCGGATGAAAGAGCCTGCTTTGAGAATGTCAAAAATT
CGATGGAATACTGAATTAGCTCAAAGAGCTCAATATATGAGTAACCAGTG
TAGACTGGGGAGTGATAATACTAATTTATGTACGTCAGCGTCACCGATGG
GCCAAATAAGTTACGCCACAATGTCCTCTGAAAAGCAATCATTGAAGTGG
ACTCAATTTATTCTCGAGTTTTATAATCAAAAATCTGGTTATAACTATGA
TACAAATACATGCACATCAAAATACTGCAATGGATATAAACAACTTGTCA
ATGCTCGAACTACAGAAATCGGATGTGCAGTTTCCAATTGCAAAAAAGGC
GAAACTTTTGCAGATTATTATTTCTGTAACTTTTATCCACCAATATATCA
ACATAGACCATATGAAAAAGGAGAAACAAAATGTGATAGTTGTTATAAAT
TGGATGATAATTATTTGTATCGTTGCACTAAAGATTTATGCGAAATTTGT
GATTCTCAAAGTTCTGATTGTCAAGGAAAAGAAGTACTGAAGCTAGCGAT
AGAAGCTGGTCTCTGTGAGGATTTGGAACCAGGTTTTTGCGAACTGAATG
CAAGCGTATGCCCAAATATTGACTTGCTTCCAGATGAATATAAAGTAATG
ATGACATCGAAGTGCAAGAAAACTTGCAAATTATGCTGATAAATGAATTT
CTTTGACATATATGAATATTGAAAAGAATAAAATATTTATGGTTGACTTT
ATTTTTTGACTCCTTTAATGTTGTAATTAATTAAATAAATTTAAGTCCGG
T
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protein sequence of SMED30034978-orf-1

>SMED30034978-orf-1 ID=SMED30034978-orf-1|Name=SMED30034978-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=346bp
NNNNINIKNRNDNINNNSSFLDRMIIFQVAFLFAVACSVAQCQYAYRPKT
ENGEFCMIPFEYQGKIFHDCTTEGDSKAWCRPASDKWGYCSNTSDFSKCQ
VAAPTSSISDVKLILDLLNNIRMKEPALRMSKIRWNTELAQRAQYMSNQC
RLGSDNTNLCTSASPMGQISYATMSSEKQSLKWTQFILEFYNQKSGYNYD
TNTCTSKYCNGYKQLVNARTTEIGCAVSNCKKGETFADYYFCNFYPPIYQ
HRPYEKGETKCDSCYKLDDNYLYRCTKDLCEICDSQSSDCQGKEVLKLAI
EAGLCEDLEPGFCELNASVCPNIDLLPDEYKVMMTSKCKKTCKLC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR036943FN_type2_sf
IPR035940CAP_sf
IPR001283CRISP-related
IPR000562FN_type2_dom
IPR014044CAP_domain
IPR013806Kringle-like
Vocabulary: cellular component
TermDefinition
GO:0005576extracellular region
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000562Fibronectin type II domainSMARTSM00059FN2_2coord: 49..90
e-value: 4.5E-6
score: 31.5
IPR000562Fibronectin type II domainPFAMPF00040fn2coord: 56..90
e-value: 1.8E-8
score: 34.5
IPR000562Fibronectin type II domainPROSITEPS51092FN2_2coord: 51..92
score: 13.508
IPR014044CAP domainSMARTSM00198SCP_3coord: 109..252
e-value: 1.2E-12
score: 58.0
IPR014044CAP domainPFAMPF00188CAPcoord: 115..244
e-value: 6.0E-16
score: 59.4
IPR036943Fibronectin type II domain superfamilyGENE3DG3DSA:2.10.10.10coord: 47..91
e-value: 2.1E-10
score: 42.2
IPR035940CAP superfamilyGENE3DG3DSA:3.40.33.10coord: 93..289
e-value: 6.6E-30
score: 106.1
IPR035940CAP superfamilySUPERFAMILYSSF55797PR-1-likecoord: 93..257
IPR001283Cysteine-rich secretory protein-relatedPANTHERPTHR10334CYSTEINE-RICH SECRETORY PROTEIN-RELATEDcoord: 102..269
NoneNo IPR availablePANTHERPTHR10334:SF344AT27079P-RELATEDcoord: 102..269
NoneNo IPR availableCDDcd05380CAP_eukcoord: 112..245
e-value: 5.07436E-22
score: 88.6655
IPR013806Kringle-like foldSUPERFAMILYSSF57440Kringle-likecoord: 47..91