cilia- and flagella-associated protein 77

Overview


Name	cilia- and flagella-associated protein 77
Smed ID	SMED30034903
Length (bp)	909

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of cilia- and flagella-associated protein 77 (SMED30034903) t-SNE clustered cells

Violin plots show distribution of expression levels for cilia- and flagella-associated protein 77 (SMED30034903) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of cilia- and flagella-associated protein 77 (SMED30034903) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for cilia- and flagella-associated protein 77 (SMED30034903) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30034903

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30034903	SMESG000042608.1	SmedASXL_001658	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30034903	SMESG000042608.1	dd_Smed_v4_5179_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30034903	SMESG000042608.1	dd_Smed_v4_5179_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30034903	SMESG000042608.1	dd_Smed_v4_5179_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30034903	SMESG000042608.1	dd_Smed_v4_5179_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30034903	SMESG000042608.1	dd_Smed_v4_5179_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neuron	SMED30034903	SMESG000042608.1	dd_Smed_v4_5179_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034903	SMESG000042608.1	Contig42199	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034903	SMESG000042608.1	Contig42199	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
head region	SMED30034903	SMESG000042608.1	dd_Smed_v6_5179_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
head region	SMED30034903	SMESG000042608.1	OX_Smed_1.0.01607	ox_Smed_v2	PMID:24238224 Kao et al., 2013
epidermis	SMED30034903	SMESG000042608.1	dd_Smed_v4_5179_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
ciliated epithelial cell	SMED30034903	SMESG000042608.1	dd_Smed_v4_5179_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Human
Match: CFAP77 (cilia and flagella associated protein 77 [Source:HGNC Symbol;Acc:HGNC:33776])

HSP 1 Score: 95.5153 bits (236), Expect = 2.445e-22
Identity = 65/225 (28.89%), Postives = 118/225 (52.44%), Query Frame = 3

Query:  111 LGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKVFGITFEKG-------SGVPEAMKWPKSYSAFTKE-SSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPRFRNRVDARISSFRNDRIRELSMK 761
            LG  R++M  N L ++ ++G+ +   + +P     GI F  G        GVPEA+     ++ F ++ +  ++  R++I +NR AV+ GLV++RE   +R  +D+   D+  +    +    + P+M +GI  RPSTP  DLL +++   W+      QK +  +++ +++   K  +TRSS LR Y  P     LW MP F+ +V   + +F  +  R+ ++K
Sbjct:   50 LGVVRDSMFQNPLIVKAELGKPRERSYSLP-----GINFNYGLYIRGLDGGVPEAIG---RWNVFKQQPTCPHELTRNYIAMNRGAVKAGLVTARENLLYRQLNDIRISDQDDRRMKKEPP-PLPPNMTFGIRARPSTPFFDLLQHRYLQLWVQEQKATQKAIKLEKKQKVVLG-KLYETRSSQLRKYKPPVKLDTLWHMPHFQ-KVGRHLDTFPTEADRQRALK 263

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Human
Match: CFAP77 (cilia and flagella associated protein 77 [Source:HGNC Symbol;Acc:HGNC:33776])

HSP 1 Score: 85.5001 bits (210), Expect = 1.862e-18
Identity = 58/191 (30.37%), Postives = 101/191 (52.88%), Query Frame = 3

Query:  216 GITFEKG-------SGVPEAM-KWPKSYSAFTKE-SSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPRFRNRVDARISSFRNDRIRELSMK 761
            GI F  G        GVPEA+ +W    + F ++ +  ++  R++I +NR AV+ GLV++RE   +R  +D+   D+  +    +    + P+M +GI  RPSTP  DLL +++   W+      QK +  +++ +++   K  +TRSS LR Y  P     LW MP F+ +V   + +F  +  R+ ++K
Sbjct:  116 GINFNYGLYIRGLDGGVPEAIGRW----NVFKQQPTCPHELTRNYIAMNRGAVKAGLVTARENLLYRQLNDIRISDQDDRRMKKEPP-PLPPNMTFGIRARPSTPFFDLLQHRYLQLWVQEQKATQKAIKLEKKQKVVLG-KLYETRSSQLRKYKPPVKLDTLWHMPHFQ-KVGRHLDTFPTEADRQRALK 299

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Human
Match: CFAP77 (cilia and flagella associated protein 77 [Source:HGNC Symbol;Acc:HGNC:33776])

HSP 1 Score: 54.299 bits (129), Expect = 3.679e-8
Identity = 37/135 (27.41%), Postives = 70/135 (51.85%), Query Frame = 3

Query:  111 LGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKVFGITFEKG-------SGVPEAMKWPKSYSAFTKE-SSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTR 491
            LG  R++M  N L ++ ++G+ +   + +P     GI F  G        GVPEA+     ++ F ++ +  ++  R++I +NR AV+ GLV++RE   +R  +D+   D+  +    +    + P+M +GI  R
Sbjct:   50 LGVVRDSMFQNPLIVKAELGKPRERSYSLP-----GINFNYGLYIRGLDGGVPEAIG---RWNVFKQQPTCPHELTRNYIAMNRGAVKAGLVTARENLLYRQLNDIRISDQDDRRMKKEPP-PLPPNMTFGIRAR 175

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Zebrafish
Match: CFAP77 (cilia and flagella associated protein 77 [Source:NCBI gene;Acc:558957])

HSP 1 Score: 117.857 bits (294), Expect = 3.248e-31
Identity = 75/228 (32.89%), Postives = 127/228 (55.70%), Query Frame = 3

Query:  111 LGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDK-VFGI-TFEKGSGVPEAMKWPKSYSAFTKESSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEK-ELWTMPRFRNRVDARISSFRNDRIRELSMKYDVGEPVS 785
            +G  R++ML+  L ++  +G+SK+     P  + VFG  T  +  GV EA+    ++   T  ++H   ERDFI LNRE V++GLV+++E   +RATHD+ +K  + +        ++SP   + +S RPSTPI +L+  K+   WL+   Q +  +++  +++  +  +  DTR++LLR    P PE    W +PRF  +V A + +FR+   R+ +M     E VS
Sbjct:    6 IGVVRDSMLHRPLLIKPTLGKSKSRGLSYPGPEFVFGTATTVQDGGVQEAIS---NWHTHTMSTTHRAAERDFIALNREGVKSGLVTAKELHQYRATHDIRRKPLTREGFRRSAPPRLSPDDSFFVSNRPSTPISELIEYKYAQRWLEE-QQVRDRVLQAHQHKKAQLGRIQDTRTTLLRKSQ-PLPEAPSTWKLPRF-QKVGAALDTFRDPEARKKAMNAHCSESVS 227

[Close]

Query:  111 LGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDK-VFGI-TFEKGSGVPEAMKWPKSYSAFTKESSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEK-ELWTMPRFRNRVDARISSFRNDRIRELSMKYDVGEPVS 785
            +G  R++ML+  L ++  +G+SK+     P  + VFG  T  +  GV EA+    ++   T  ++H   ERDFI LNRE V++GLV+++E   +RATHD+ +K  + +        ++SP   + +S RPSTPI +L+  K+   WL+   Q +  +++  +++  +  +  DTR++LLR    P PE    W +PRF  +V A + +FR+   R+ +M     E VS
Sbjct:    6 IGVVRDSMLHRPLLIKPTLGKSKSRGLSYPGPEFVFGTATTVQDGGVQEAIS---NWHTHTMSTTHRAAERDFIALNREGVKSGLVTAKELHQYRATHDIRRKPLTREGFRRSAPPRLSPDDSFFVSNRPSTPISELIEYKYAQRWLEE-QQVRDRVLQAHQHKKAQLGRIQDTRTTLLRKSQ-PLPEAPSTWKLPRF-QKVGAALDTFRDPEARKKAMNAHCSESVS 227

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Xenopus
Match: fgfr4 (fibroblast growth factor receptor 4 [Source:Xenbase;Acc:XB-GENE-478699])

HSP 1 Score: 99.7525 bits (247), Expect = 6.946e-24
Identity = 68/217 (31.34%), Postives = 117/217 (53.92%), Query Frame = 3

Query:   57 KKMISLDNEEFVDTATGILGNKRETMLNNCLHMRDKVGRSKTFCHHVPN-DKVFGI-TFEKGSGVPEAMKWPKSYSAFTKESSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKK--DKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPRF 695
            K+  +++NE F        G  R++M +N L  R ++G  +   + +P  D  +G+ ++ +  GV  A+     +++   ++   K ERD++ LNREAV++GLV++ E   +R  H + +   +  TK   ++    +  +M +GISTRPSTPI DLL +++Q  W++     Q + +  +    IK  K  +TR+SLLR Y  P     LW +PRF
Sbjct:   75 KRESAMENERF--------GVVRDSMEDNTLLQRAQLGTVRRASYPLPGADFTYGVKSYIQDGGVALALG---QWNSIEAKTRLRKLERDYVALNREAVKSGLVTAHEHQVYRNHHQIWRPLHEGCTKPQGLN----LPTNMTFGISTRPSTPIGDLLDHRYQRLWVEQQRVAQ-EALLLKSKEKIKKSKVQNTRTSLLRRYQPPGDPAPLWHLPRF 275

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Xenopus
Match: fgfr4 (fibroblast growth factor receptor 4 [Source:Xenbase;Acc:XB-GENE-478699])

HSP 1 Score: 90.8929 bits (224), Expect = 4.822e-21
Identity = 60/190 (31.58%), Postives = 103/190 (54.21%), Query Frame = 3

Query:  138 NNCLHMRDKVGRSKTFCHHVPN-DKVFGI-TFEKGSGVPEAMKWPKSYSAFTKESSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVK--KDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPRF 695
            N   H + ++G  +   + +P  D  +G+ ++ +  GV  A+     +++   ++   K ERD++ LNREAV++GLV++ E   +R  H + +   +  TK   ++    +  +M +GISTRPSTPI DLL +++Q  W++     Q + +  +    IK  K  +TR+SLLR Y  P     LW +PRF
Sbjct:   36 NPVPHNQAQLGTVRRASYPLPGADFTYGVKSYIQDGGVALALG---QWNSIEAKTRLRKLERDYVALNREAVKSGLVTAHEHQVYRNHHQIWRPLHEGCTKPQGLN----LPTNMTFGISTRPSTPIGDLLDHRYQRLWVEQQRVAQ-EALLLKSKEKIKKSKVQNTRTSLLRRYQPPGDPAPLWHLPRF 217

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Mouse
Match: Cfap77 (cilia and flagella associated protein 77 [Source:MGI Symbol;Acc:MGI:2685669])

HSP 1 Score: 102.834 bits (255), Expect = 4.076e-25
Identity = 72/237 (30.38%), Postives = 125/237 (52.74%), Query Frame = 3

Query:   90 VDTATGI----LGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKVFGITFEKG-------SGVPEAMKWPKSYSAFTKE-SSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDL-MEKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPRFRNRVDARISSFRNDRIRELSMK 761
            VD  TG+    LG  R++M  N L ++ ++G+ +     +P     GI F  G        GVPEA+     ++ F ++ +  ++  R++I +NR AV+ GLV++RE   +R  +D+   D+  +         I P+M +GI +RPSTP  DLL +++Q  W+      Q+ + MEK++  ++      +TRSS LR Y  P     LW MP F+ +V + +++F  +  R+ ++K
Sbjct:   39 VDIRTGMENERLGVVRDSMFQNPLIVKAELGKPRERSCSLP-----GINFNYGLYIRGLDGGVPEAIG---HWNVFKQQPTCPHELTRNYIAMNRGAVKAGLVTARENMLYRELNDIRINDQEDRRQKEPP--PIPPNMTFGIRSRPSTPFFDLLQHRYQQLWVQEQKATQQAIKMEKKQKVILGKL--YETRSSQLRKYKPPVKLDALWHMPHFK-KVASHLATFPTEADRQRALK 262

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Mouse
Match: Cfap77 (cilia and flagella associated protein 77 [Source:MGI Symbol;Acc:MGI:2685669])

HSP 1 Score: 98.5969 bits (244), Expect = 1.032e-23
Identity = 67/215 (31.16%), Postives = 112/215 (52.09%), Query Frame = 3

Query:   90 VDTATGI----LGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKVFGITFEKG-------SGVPEAMKWPKSYSAFTKE-SSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPRFR 698
            VD  TG+    LG  R++M  N L ++ ++G+ +     +P     GI F  G        GVPEA+     ++ F ++ +  ++  R++I +NR AV+ GLV++RE   +R  +D+   D+  +         I P+M +GI +RPSTP  DLL +++Q  W+      Q+ +  +++ ++I   K  +TRSS LR Y  P     LW MP F+
Sbjct:   39 VDIRTGMENERLGVVRDSMFQNPLIVKAELGKPRERSCSLP-----GINFNYGLYIRGLDGGVPEAIG---HWNVFKQQPTCPHELTRNYIAMNRGAVKAGLVTARENMLYRELNDIRINDQEDRRQ--KEPPPIPPNMTFGIRSRPSTPFFDLLQHRYQQLWVQEQKATQQAIKMEKKQKVILG-KLYETRSSQLRKYKPPVKLDALWHMPHFK 242

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. UniProt/SwissProt
Match: sp|A2BFC9|CFA77_DANRE (Cilia- and flagella-associated protein 77 OS=Danio rerio OX=7955 GN=cfap77 PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 2.452e-30
Identity = 75/228 (32.89%), Postives = 127/228 (55.70%), Query Frame = 3

Query:  111 LGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDK-VFGI-TFEKGSGVPEAMKWPKSYSAFTKESSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEK-ELWTMPRFRNRVDARISSFRNDRIRELSMKYDVGEPVS 785
            +G  R++ML+  L ++  +G+SK+     P  + VFG  T  +  GV EA+    ++   T  ++H   ERDFI LNRE V++GLV+++E   +RATHD+ +K  + +        ++SP   + +S RPSTPI +L+  K+   WL+   Q +  +++  +++  +  +  DTR++LLR    P PE    W +PRF  +V A + +FR+   R+ +M     E VS
Sbjct:    6 IGVVRDSMLHRPLLIKPTLGKSKSRGLSYPGPEFVFGTATTVQDGGVQEAIS---NWHTHTMSTTHRAAERDFIALNREGVKSGLVTAKELHQYRATHDIRRKPLTREGFRRSAPPRLSPDDSFFVSNRPSTPISELIEYKYAQRWLEE-QQVRDRVLQAHQHKKAQLGRIQDTRTTLLRKSQ-PLPEAPSTWKLPRF-QKVGAALDTFRDPEARKKAMNAHCSESVS 227

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. UniProt/SwissProt
Match: sp|Q6ZQR2|CFA77_HUMAN (Cilia- and flagella-associated protein 77 OS=Homo sapiens OX=9606 GN=CFAP77 PE=1 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 8.943e-18
Identity = 58/191 (30.37%), Postives = 101/191 (52.88%), Query Frame = 3

Query:  216 GITFEKG-------SGVPEAM-KWPKSYSAFTKE-SSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPRFRNRVDARISSFRNDRIRELSMK 761
            GI F  G        GVPEA+ +W    + F ++ +  ++  R++I +NR AV+ GLV++RE   +R  +D+   D+  +    +    + P+M +GI  RPSTP  DLL +++   W+      QK +  +++ +++   K  +TRSS LR Y  P     LW MP F+ +V   + +F  +  R+ ++K
Sbjct:  116 GINFNYGLYIRGLDGGVPEAIGRW----NVFKQQPTCPHELTRNYIAMNRGAVKAGLVTARENLLYRQLNDIRISDQDDRRMKKEPP-PLPPNMTFGIRARPSTPFFDLLQHRYLQLWVQEQKATQKAIKLEKKQKVVLG-KLYETRSSQLRKYKPPVKLDTLWHMPHFQ-KVGRHLDTFPTEADRQRALK 299

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. TrEMBL
Match: R7T8V9 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_21391 PE=4 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 2.646e-44
Identity = 90/242 (37.19%), Postives = 142/242 (58.68%), Query Frame = 3

Query:   78 NEEFVDTATGILGNKRETMLNNCLHMRDKVGR--SKTFCHHVPNDKVFGI-TFEKGSGVPEAMK-WPKSYSAFTKES---SHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNA-IDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDH-----LSQKQKDLMEKERNRMIKSFKPS---DTRSSLLRTYMVPAPEKELWTMPRFRNRVDARISSFRNDRIRELS 755
             E F  TATG+LG KR+TML+N LH+R+K+GR   + + ++      +G+    +  G  EA+  WP     +            ERDFI LN+ AV NG+ +S++ + FRAT+DV KK + TKS   +   RKI  +MVYG++TRP+TPI  L+ +++Q++WL+      L+Q+ K  M++ R + +   KP    +TR+SL+R Y VP  +  LW +P+F  +    + +F  DR R+L+
Sbjct:    5 GEYFEHTATGVLGEKRDTMLHNQLHLRNKLGRPMERGYINYPGEQYRYGVPNATRDGGASEALSGWPNRGGLYFHRKDTIGDEPLERDFISLNKSAVLNGITNSQDQTQFRATNDVRKKVQDTKSEQRVKTPRKIPVNMVYGVATRPATPIDQLIQHRYQDKWLEETRSADLAQRAKSEMQRRRIQALSRLKPGKVCNTRTSLMRVYQVPVEDAPLWHLPKFAKKAQPHLKTFATDRARDLA 246

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. TrEMBL
Match: A0A267EZS7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034181g1 PE=4 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 5.764e-44
Identity = 88/244 (36.07%), Postives = 138/244 (56.56%), Query Frame = 3

Query:   81 EEFVDTATGILGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKVFGI-TFEKGSGVPEAMKWPKSYSAFTKESSH-----NKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKK-DKSTKSNAIDGNRKISP-SMVYGISTRPSTPIHDLLANKFQNEWL-DHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPE---KELWTMPRFRNRVDARISSFRNDRIRELSMKYDVGE 776
            + +  TATG+LG+KR TML N +H R ++ R+KT CHH P +   GI T    S +P+A  W  +  A    SS       +  +DF+ LNR+A R+G  + RE   FRA HD  K+ ++  +++        +P    +G+  RPSTPIH+L+A KFQN+W+ D +S  Q       +  ++   +P +TR+S+LR+Y++PA +     LW MPRF     +R+ +FR++R R L+ +    E
Sbjct:    6 DRYTYTATGVLGDKRPTMLENEIHQRSQLARAKTHCHHFPPNMTMGISTARDHSALPKAFSWTDAPVAANAGSSSLLSRPKQQPKDFVSLNRDAARSGYATMRENRDFRAMHDYRKQPERDRRASGTGAATSRAPVDAAHGVGNRPSTPIHELIAYKFQNDWIRDQVSGNQARAAATPKKPLM--CQPYETRASMLRSYLLPAEQHASASLWKMPRFERSARSRLDTFRSERQRSLAFELHEAE 247

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. TrEMBL
Match: A0A1I8I157 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034181g2 PE=4 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 7.454e-44
Identity = 88/244 (36.07%), Postives = 138/244 (56.56%), Query Frame = 3

Query:   81 EEFVDTATGILGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKVFGI-TFEKGSGVPEAMKWPKSYSAFTKESSH-----NKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKK-DKSTKSNAIDGNRKISP-SMVYGISTRPSTPIHDLLANKFQNEWL-DHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPE---KELWTMPRFRNRVDARISSFRNDRIRELSMKYDVGE 776
            + +  TATG+LG+KR TML N +H R ++ R+KT CHH P +   GI T    S +P+A  W  +  A    SS       +  +DF+ LNR+A R+G  + RE   FRA HD  K+ ++  +++        +P    +G+  RPSTPIH+L+A KFQN+W+ D +S  Q       +  ++   +P +TR+S+LR+Y++PA +     LW MPRF     +R+ +FR++R R L+ +    E
Sbjct:    6 DRYTYTATGVLGDKRPTMLENEIHQRSQLARAKTHCHHFPPNMTMGISTARDHSALPKAFSWTDAPVAANAGSSSLLNRPKQQPKDFVSLNRDAARSGYATMRENRDFRAMHDYRKQPERDRRASGTGAATSRAPVDAAHGVGNRPSTPIHELIAYKFQNDWIRDQVSGNQARAAATPKKPLM--CQPYETRASMLRSYLLPAEQHTSASLWKMPRFERSARSRLDTFRSERQRSLAFELHEAE 247

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. TrEMBL
Match: A0A1I8JH59 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006478g1 PE=4 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.343e-43
Identity = 88/244 (36.07%), Postives = 136/244 (55.74%), Query Frame = 3

Query:   81 EEFVDTATGILGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKVFGI-TFEKGSGVPEAMKWPKSYSAFTKESSH-----NKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSM--VYGISTRPSTPIHDLLANKFQNEWL-DHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPE---KELWTMPRFRNRVDARISSFRNDRIRELSMKYDVGE 776
            + +  TATG+LG+KR TML N +H R ++ R+KT CHH P +   GI T    + +P+A  W  +  A    SS       +  +DF+ LNR+A R+G  + RE   FRA HD  K+ +  +  +  G     P +   +G+  RPSTPIH+L+A KFQN+W+ D +S  Q          ++   +P +TR+S+LR+Y++PA +     LW MPRF     +R+ +FR++R R L+ +    E
Sbjct:    6 DRYTYTATGVLGDKRPTMLENEIHQRSQLARAKTHCHHFPPNMTMGISTTRDHTALPKAFSWTDAPVAANAGSSSLLSRPKQQPKDFVSLNRDAARSGYATMRENRDFRAMHDYRKQPERDRRASGTGAATSRPPVDAAHGVGNRPSTPIHELIAYKFQNDWIRDQVSGNQARAAATPMKPLM--CQPYETRASMLRSYLLPAEQHASASLWKMPRFERSARSRLDTFRSERQRSLAFELHEAE 247

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. TrEMBL
Match: A0A1I8ITQ7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 1.668e-43
Identity = 88/244 (36.07%), Postives = 138/244 (56.56%), Query Frame = 3

Query:   81 EEFVDTATGILGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKVFGI-TFEKGSGVPEAMKWPKSYSAFTKESSH-----NKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKK-DKSTKSNAIDGNRKISP-SMVYGISTRPSTPIHDLLANKFQNEWL-DHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPE---KELWTMPRFRNRVDARISSFRNDRIRELSMKYDVGE 776
            + +  TATG+LG+KR TML N +H R ++ R+KT CHH P +   GI T    S +P+A  W  +  A    SS       +  +DF+ LNR+A R+G  + RE   FRA HD  K+ ++  +++        +P    +G+  RPSTPIH+L+A KFQN+W+ D +S  Q       +  ++   +P +TR+S+LR+Y++PA +     LW MPRF     +R+ +FR++R R L+ +    E
Sbjct:    6 DRYTYTATGVLGDKRPTMLENEIHQRSQLARAKTHCHHFPPNMTMGISTARDHSALPKAFSWTDAPVAANAGSSSLLNRPKQQPKDFVSLNRDAARSGYATMRENRDFRAMHDYRKQPERDRRASGTGAATSRAPVDAAHGVGNRPSTPIHELIAYKFQNDWIRDQVSGNQARAAATPKKPLM--CQPYETRASMLRSYLLPAEQHTSASLWKMPRFERSARSRLDTFRSERQRSLAFELHEAE 247

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Cavefish
Match: si:ch211-215c18.3 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000027.1:2410739:2426522:-1 gene:ENSAMXG00000009656.2 transcript:ENSAMXT00000009927.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:si:ch211-215c18.3)

HSP 1 Score: 94.7449 bits (234), Expect = 6.309e-23
Identity = 60/200 (30.00%), Postives = 106/200 (53.00%), Query Frame = 3

Query:  165 VGRSKTFCHHVPN-DKVFGI-TFEKGSGVPEAMKWPKSYSAFTKESSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPRFRNRVDARISSFRNDRIRELSM 758
            +G+S++     P  D V+G  T  +  GV E   W    ++    SS  K ERDF+ LNRE V++G+V++R+  H+RATHD+ +   + +        +I   + +G+ T PS  I D+L  +F  +WL+    K+K L+ + + + ++  +  DTR++LL+           W +PRF  ++   + +FR+   RE +M
Sbjct:    3 LGKSQSRRLSCPGPDFVYGAPTIVRDGGVAEISNW--DTASLNTSSSARKAERDFVALNREGVKSGMVTARDLRHYRATHDIQRPIVTDRHPPT----RIPQDIPFGVPT-PSESIADMLQYRFAQKWLEEQQAKEKTLLGR-KPKKVQLGRIRDTRTTLLQKARPLEQPPSTWQLPRF-QQIGPALDTFRDPEARERAM 193

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Nematostella
Match: EDO43313 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYZ2])

HSP 1 Score: 125.561 bits (314), Expect = 1.436e-34
Identity = 75/232 (32.33%), Postives = 127/232 (54.74%), Query Frame = 3

Query:  111 LGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKVFGITF-----EKGSGVPEAMKWPKSYSAFTKESSHN--KTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPRFRNRVDARISSFRNDRIRELSMKYDVGEPVS 785
            LG  R+++LNN L  + ++GR    C  +P D   G T+      K SG  +A+ W    SA  ++ SH   K  RDF  LN+ AV+ GL +++E   +RATHDV +KD S         +++ P+MV+G+ T+PSTP++DLL +K+Q+ WL+   + +  +  +++ +        +TR+SLLR Y  P     LW M +F N++     +FR+++ +  +  +   +  S
Sbjct:    9 LGVHRDSLLNNPLLQKTELGRPLRRCFTLPPD---GFTYGKPNGNKDSGAADALVW---RSAPPQQFSHKEKKKPRDFQSLNKSAVQVGLTTAQEHFQYRATHDVRRKD-SGNERTTQKIKRLPPTMVFGVPTKPSTPVYDLLGHKYQDRWLEERRKAEDGMRARQKQKRHVDKNIYETRASLLRKYQPPVDPPPLWHMAKF-NKIPPVTVTFRSEKAKSAAFDHHATDSTS 232

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Medaka
Match: ENSORLT00000029839.1 (cilia and flagella associated protein 77 [Source:NCBI gene;Acc:101172143])

HSP 1 Score: 74.7146 bits (182), Expect = 1.659e-15
Identity = 61/221 (27.60%), Postives = 111/221 (50.23%), Query Frame = 3

Query:  111 LGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKV-FGITFEKGSGVPEAMKWPKSYSAFTKESSHNKTERDFIGLNREAVRNGLVSSREFSHFRA--THDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLM-EKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPRFRNRVDARISSFRNDRIRELSMK 761
            LG  R++ML N L ++  +GR+ +     P   V  GI       V E +    S+              +F+ LNR+AVR G+V+S+E S +RA      ++   S+K N     R + P +++G++ +PS  + DLL++ + + WL+      K+L   +E  + +K   P++TR+ LLR        K+   +P F  +V   +++FR+ + RE +++
Sbjct:    6 LGVVRKSMLTNPLLIKPPLGRACSRGLFFPEPDVPCGIRSHCNGSVAEVL----SHWEVQPRPKELLQSTNFMSLNRDAVRAGVVTSKEMSQYRAQRAKAPIQSWSSSKDNPNASGRSV-PDIMFGVTHKPSDKMADLLSHTYGHLWLE------KELSRPREPGKRVKPLVPAETRTVLLRKSRSLPIIKQPVKLPEF-AQVAPALNTFRDPKDREQALR 214

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. Planmine SMEST
Match: SMESG000042608.1 (SMESG000042608.1)

HSP 1 Score: 505.753 bits (1301), Expect = 0.000e+0
Identity = 242/244 (99.18%), Postives = 243/244 (99.59%), Query Frame = 3

Query:   63 MISLDNEEFVDTATGILGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKVFGITFEKGSGVPEAMKWPKSYSAFTKESSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPRFRNRVDARISSFRNDRIRELSMKYDVGEPVSGIA 794
            MISLDNEEFVDTATGILGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKVFGITFEKGSGVPEAMKWPKSYSAFTKESSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAID NRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPR+RNRVDARISSFRNDRIRELSMKYDVGEPVSGIA
Sbjct:    1 MISLDNEEFVDTATGILGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKVFGITFEKGSGVPEAMKWPKSYSAFTKESSHNKTERDFIGLNREAVRNGLVSSREFSHFRATHDVVKKDKSTKSNAIDANRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPRYRNRVDARISSFRNDRIRELSMKYDVGEPVSGIA 244

[Close]

BLAST of cilia- and flagella-associated protein 77 vs. Planmine SMEST
Match: SMESG000064718.1 (SMESG000064718.1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.851e-15
Identity = 66/241 (27.39%), Postives = 110/241 (45.64%), Query Frame = 3

Query:   54 LKKMISLDNEEFVDTATGILGNKRETMLNNCLHMRDKVGRSKTFCH-HVPNDKVFGITFE-KGSGVPEAMKWPKSYSAFTKESSHNKTERDFIGLNREAVRNGLV--SSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHDLLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPAPEKELWTMPRFRNRVDARISSFRNDRIRELSMKY 764
             KK    D  ++   A G+LG  RE ML      R   G+  T      P    +G+ +    +GV + M+               K+    I   +EA+ NG+   +S+ + H     D V      ++       K    + +G   RPS+P+ D++++K++N+W +   Q+     ++ + +  K  +P D+ +S+LRTY  P P KELW MP+F +RV AR+  F  D  R+   KY
Sbjct:    3 FKKTTLKDLMKYEYVAPGLLGLGRENMLKMETLQRAPTGKFVTRGRPKQPPGFRYGMLYPYDPNGVAKCMQ------------QKVKSNPGGIVARKEAMDNGIKMDTSKHWPHKGKNMDPVAMYWQKRTV------KFPKCITFGRPFRPSSPMGDVMSHKYKNDWDEWAIQENLKATDEWKRKRSKVTRPWDSIASVLRTYTKPKPVKELWHMPKF-SRVPARVLPFWEDDDRKAIKKY 224

The following BLAST results are available for this feature:

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Human
BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Celegans
BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Fly
BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Zebrafish
BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Xenopus
BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Mouse
BLAST of cilia- and flagella-associated protein 77 vs. UniProt/SwissProt
BLAST of cilia- and flagella-associated protein 77 vs. TrEMBL
BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Cavefish
BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Sea Lamprey
BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Yeast
BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Nematostella
BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Medaka
BLAST of cilia- and flagella-associated protein 77 vs. Planmine SMEST

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3


Match Name	E-value	Identity	Description
CFAP77	2.445e-22	28.89	cilia and flagella associated protein 77 [Source:H...	[more]
CFAP77	1.862e-18	30.37	cilia and flagella associated protein 77 [Source:H...	[more]
CFAP77	3.679e-8	27.41	cilia and flagella associated protein 77 [Source:H...	[more]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
CFAP77	3.248e-31	32.89	cilia and flagella associated protein 77 [Source:N...	[more]
CFAP77	3.248e-31	32.89	cilia and flagella associated protein 77 [Source:N...	[more]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2


Match Name	E-value	Identity	Description
fgfr4	6.946e-24	31.34	fibroblast growth factor receptor 4 [Source:Xenbas...	[more]
fgfr4	4.822e-21	31.58	fibroblast growth factor receptor 4 [Source:Xenbas...	[more]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Cfap77	4.076e-25	30.38	cilia and flagella associated protein 77 [Source:M...	[more]
Cfap77	1.032e-23	31.16	cilia and flagella associated protein 77 [Source:M...	[more]

BLAST of cilia- and flagella-associated protein 77 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2


Match Name	E-value	Identity	Description
sp\|A2BFC9\|CFA77_DANRE	2.452e-30	32.89	Cilia- and flagella-associated protein 77 OS=Danio...	[more]
sp\|Q6ZQR2\|CFA77_HUMAN	8.943e-18	30.37	Cilia- and flagella-associated protein 77 OS=Homo ...	[more]

BLAST of cilia- and flagella-associated protein 77 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
R7T8V9	2.646e-44	37.19	Uncharacterized protein OS=Capitella teleta OX=283...	[more]
A0A267EZS7	5.764e-44	36.07	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A1I8I157	7.454e-44	36.07	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A1I8JH59	1.343e-43	36.07	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A1I8ITQ7	1.668e-43	36.07	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
si:ch211-215c18.3	6.309e-23	30.00	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO43313	1.436e-34	32.33	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of cilia- and flagella-associated protein 77 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSORLT00000029839.1	1.659e-15	27.60	cilia and flagella associated protein 77 [Source:N...	[more]

BLAST of cilia- and flagella-associated protein 77 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000042608.1	0.000e+0	99.18	SMESG000042608.1	[more]
SMESG000064718.1	4.851e-15	27.39	SMESG000064718.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30034903 ID=SMED30034903|Name=cilia- and flagella-associated protein 77|organism=Schmidtea mediterranea sexual|type=transcript|length=909bp
CATATTTACATTGTTTTAACTAAATCATCCATAGCGAAATAAAATCCGAA
AACTTAAAGAAAATGATAAGTTTGGATAATGAAGAATTTGTTGATACCGC
TACTGGTATTTTGGGTAATAAGCGAGAAACTATGTTGAATAATTGCTTAC
ATATGAGAGACAAAGTTGGTAGATCAAAGACATTTTGTCACCACGTTCCA
AATGATAAAGTTTTCGGAATAACATTCGAAAAAGGCAGTGGAGTACCAGA
AGCTATGAAATGGCCAAAGTCATACAGTGCTTTTACAAAAGAATCTTCAC
ATAATAAAACTGAAAGAGATTTTATTGGATTGAACAGAGAAGCAGTTCGT
AATGGATTGGTAAGTTCTCGAGAATTCAGTCACTTCAGGGCGACTCACGA
TGTCGTCAAGAAAGATAAATCAACTAAATCAAACGCCATTGATGGAAATC
GAAAAATATCACCAAGTATGGTATATGGAATAAGTACTAGGCCGTCAACC
CCTATTCATGATTTGCTAGCTAATAAATTTCAGAATGAATGGCTGGACCA
TTTAAGTCAAAAACAAAAGGATTTAATGGAAAAAGAAAGAAACAGAATGA
TAAAATCTTTTAAACCAAGTGATACTCGATCCTCATTATTGAGGACATAC
ATGGTTCCGGCACCAGAGAAAGAATTATGGACAATGCCACGATTTAGAAA
CAGGGTTGATGCTCGTATAAGCTCGTTTCGAAACGACCGAATAAGGGAAT
TGTCAATGAAATATGATGTTGGGGAACCAGTTTCGGGAATCGCATAAGAA
ATTGTATTACAATTGTTTCAGTTTGTTTATCATTTGTGCTTTGACATCAT
GAAATTGTATACTTAGAAACCACTTTATCACTTTCTAAGATAATTATTGT
TGACTTTTT

protein sequence of SMED30034903-orf-1

>SMED30034903-orf-1 ID=SMED30034903-orf-1|Name=SMED30034903-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=245bp
MISLDNEEFVDTATGILGNKRETMLNNCLHMRDKVGRSKTFCHHVPNDKV
FGITFEKGSGVPEAMKWPKSYSAFTKESSHNKTERDFIGLNREAVRNGLV
SSREFSHFRATHDVVKKDKSTKSNAIDGNRKISPSMVYGISTRPSTPIHD
LLANKFQNEWLDHLSQKQKDLMEKERNRMIKSFKPSDTRSSLLRTYMVPA
PEKELWTMPRFRNRVDARISSFRNDRIRELSMKYDVGEPVSGIA*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **cellular component**
Term	Definition
GO:0031514	motile cilium
GO:0005929	cilium
GO:0042995	cell projection

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000020	protonephridia
PLANA:0000034	epidermis
PLANA:0000099	neuron
PLANA:0000106	ciliated epithelial cell
PLANA:0000418	head
PLANA:0002032	epidermal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR029147	CFAP77

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029147	Cilia- and flagella-associated protein 77	PFAM	PF14825	DUF4483	coord: 21..170 e-value: 2.6E-38 score: 131.3
IPR029147	Cilia- and flagella-associated protein 77	PANTHER	PTHR28617	FAMILY NOT NAMED	coord: 17..233

Main menu

cilia- and flagella-associated protein 77

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Owlery

blazegraph-runner