SMED30034869

Overview
NameSMED30034869
Smed IDSMED30034869
Length (bp)501
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30034869

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 22

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30034869h1SMcG0014521 SmedASXL_072889SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30034869h1SMcG0014521 SmedASXL_072889SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30034869h1SMcG0014523 SmedASXL_072889SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30034869h1SMcG0014523 SmedASXL_072889SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30034869h1SMcG0014523 SmedASXL_014255SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30034869h1SMcG0014523 SmedASXL_014255SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30034869h1SMcG0014523 SmedASXL_014254SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30034869h1SMcG0014523 SmedASXL_014254SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30034869h1SMcG0014523 SmedASXL_013078SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30034869h1SMcG0014523 SmedASXL_013078SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30034869h1SMcG0014523 SmedASXL_011698SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30034869h1SMcG0014523 SmedASXL_011698SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30034869h1SMcG0014523 dd_Smed_v6_1382_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30034869h1SMcG0014523 dd_Smed_v6_1119_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
mouthSMED30034869h1SMcG0014523 dd_Smed_v6_5849_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult colorimetric in situ hybridization evidence
parapharyngeal regionSMED30034869h1SMcG0014523 dd_Smed_v6_5849_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult colorimetric in situ hybridization evidence
parapharyngeal regionSMED30034869h1SMcG0014523 dd_Smed_v6_5849_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
tail regionSMED30034869h1SMcG0014523 dd_Smed_v6_5849_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult colorimetric in situ hybridization evidence
parapharyngeal regionSMED30034869h1SMcG0014523 dd_Smed_v6_8432_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30034869h1SMcG0014523 dd_Smed_v6_8432_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult colorimetric in situ hybridization evidence
head regionSMED30034869h1SMcG0014523 OX_Smed_1.0.00911ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30034869h1SMcG0014523 OX_Smed_1.0.21355ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30034869 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005992:22401..22901 -2496
v31.005992:22659..23159 -2487
v31.005992:22143..22643 -2487
v31.005992:22917..23415 -2441
v31.005992:23175..23674 -1888
v31.005992:23433..23900 -1134
Homology
[Close]
BLAST of SMED30034869 vs. TrEMBL
Match: A0A1S6KMJ1 (Uncharacterized protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 1.861e-59
Identity = 111/166 (66.87%), Postives = 134/166 (80.72%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            +V IKN K  E NLK I+ETSNK+I+ L E+NWE LVKYID+S+R EVI KI TFIT V+NKQYEKID +LINW+  II   R+V++VHIKNEK+T +N K IT+TSNKY+NFL ELNWE LVK I+E+ RPEV KKI TFITLVQNKQYEKID +L+N +  II+
Sbjct:  244 EVQIKNTKTEEVNLKEISETSNKFIDILVEINWEELVKYIDQSNRLEVINKIKTFITFVKNKQYEKIDTNLINWINHIITIFRQVKEVHIKNEKITNDNRKLITDTSNKYVNFLIELNWEELVKQIEETKRPEVTKKIKTFITLVQNKQYEKIDSELLNLIVEIIN 409

HSP 2 Score: 186.037 bits (471), Expect = 2.167e-52
Identity = 97/152 (63.82%), Postives = 119/152 (78.29%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEK 457
            +++I N    ++NLK ITE S +YI  L EL WE L+KYID  +RPEV+ KI TFI  VQNKQ+E+ID  L+NW+T II TLR+V+Q+HIKNE+VT EN K ITETSNKY+N+L +L WE L+KYIDE +RPE  KKI TFITLVQNKQYEK
Sbjct:  501 EINITNTNNKQQNLKVITELSIQYIQLLNELKWEELIKYIDAKNRPEVVDKIKTFIKFVQNKQFEQIDTGLLNWITRIITTLREVKQIHIKNEEVTTENKKIITETSNKYVNYLIDLKWEELIKYIDEKNRPEFTKKIKTFITLVQNKQYEK 652

HSP 3 Score: 179.874 bits (455), Expect = 5.478e-50
Identity = 97/165 (58.79%), Postives = 126/165 (76.36%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            +VHIKNEK+T +N K IT+TSNKY+NFL ELNWE LVK I+E+ RPEV KKI TFITLVQNKQYEKID +L+N +  II+TLR+V+ + I+NEK      + + + ++ Y+  L E+ WE L+KYIDE SRPE+IKKI  F+ L QNK+Y++ID  L+NWL  II
Sbjct:  330 EVHIKNEKITNDNRKLITDTSNKYVNFLIELNWEELVKQIEETKRPEVTKKIKTFITLVQNKQYEKIDSELLNLIVEIINTLREVK-IKIENEKSNLSYRERVIKLTDSYLEILNEVKWEELMKYIDEQSRPEIIKKIKNFVILAQNKKYDEIDSTLLNWLNQII 493

HSP 4 Score: 176.022 bits (445), Expect = 1.182e-48
Identity = 105/159 (66.04%), Postives = 127/159 (79.87%), Query Frame = 2 
Query:   29 TEENL---KTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            TEEN+   KTIT+ SN ++  L EL WE L+KYIDESSRPEV+ KI TF+TLV+NKQYEKID+DLI W+T II TL+K ++V IKN K  E NLK I+ETSNK+I+ L E+NWE LVKYID+S+R EVI KI TFIT V+NKQYEKID +LINW+  II
Sbjct:  164 TEENIIDIKTITDKSNNFLITLTELKWEELLKYIDESSRPEVLNKIKTFVTLVENKQYEKIDIDLITWITEIITTLKKAKEVQIKNTKTEEVNLKEISETSNKFIDILVEINWEELVKYIDQSNRLEVINKIKTFITFVKNKQYEKIDTNLINWINHII 322

HSP 5 Score: 163.31 bits (412), Expect = 7.493e-44
Identity = 84/165 (50.91%), Postives = 116/165 (70.30%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            ++ I+NEK      + + + ++ Y+  L E+ WE L+KYIDE SRPE+IKKI  F+ L QNK+Y++ID  L+NWL  II  LR++R+++I N    ++NLK ITE S +YI  L EL WE L+KYID  +RPEV+ KI TFI  VQNKQ+E+ID  L+NW+T II
Sbjct:  415 KIKIENEKSNLSYRERVIKLTDSYLEILNEVKWEELMKYIDEQSRPEIIKKIKNFVILAQNKKYDEIDSTLLNWLNQIIVMLRRIREINITNTNNKQQNLKVITELSIQYIQLLNELKWEELIKYIDAKNRPEVVDKIKTFIKFVQNKQFEQIDTGLLNWITRII 579

HSP 6 Score: 90.1225 bits (222), Expect = 8.319e-18
Identity = 60/129 (46.51%), Postives = 83/129 (64.34%), Query Frame = 2 
Query:   95 NWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEEN---LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDL 472
            NW  ++       R + I +I T I LV+  + +KID   + WL   ++ + KV+++       TEEN   +KTIT+ SN ++  L EL WE L+KYIDESSRPEV+ KI TF+TLV+NKQYEKID+DL
Sbjct:  109 NWAKIINETKNIDRVKWIDRINTIIKLVKLNEVDKID---LTWLKE-LEKIFKVKEIP-----STEENIIDIKTITDKSNNFLITLTELKWEELLKYIDESSRPEVLNKIKTFVTLVENKQYEKIDIDL 228
[Close]
BLAST of SMED30034869 vs. Planmine SMEST
Match: SMESG000008843.1 (SMESG000008843.1)

HSP 1 Score: 315.849 bits (808), Expect = 3.959e-100
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID
Sbjct: 2747 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 2912

HSP 2 Score: 315.849 bits (808), Expect = 3.959e-100
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID
Sbjct: 2833 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 2998

HSP 3 Score: 315.849 bits (808), Expect = 3.959e-100
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID
Sbjct: 2919 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 3084

HSP 4 Score: 314.694 bits (805), Expect = 1.159e-99
Identity = 165/166 (99.40%), Postives = 165/166 (99.40%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFI LVQNKQYEKIDVDLINWLTTIID
Sbjct: 3005 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFIALVQNKQYEKIDVDLINWLTTIID 3170

HSP 5 Score: 275.404 bits (703), Expect = 4.851e-86
Identity = 144/166 (86.75%), Postives = 153/166 (92.17%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            Q+HIKNE+VT EN K ITETSNKY+N+L +L WE L+KYIDE +RPE  KKI TFITLVQNKQYEKIDVDLINWLT II TLRK+R+VHIKN KVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID
Sbjct: 2508 QIHIKNEEVTTENKKIITETSNKYVNYLIDLKWEELIKYIDEKNRPEFTKKIKTFITLVQNKQYEKIDVDLINWLTEIIPTLRKIREVHIKNAKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 2673

HSP 6 Score: 271.166 bits (692), Expect = 1.812e-84
Identity = 147/166 (88.55%), Postives = 147/166 (88.55%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPE                   IDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID
Sbjct: 2680 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPE-------------------IDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 2826

HSP 7 Score: 267.7 bits (683), Expect = 3.003e-83
Identity = 145/166 (87.35%), Postives = 146/166 (87.95%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            +VHIKN KVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPE                   IDVDLINWLTTIID
Sbjct: 2594 EVHIKNAKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPE-------------------IDVDLINWLTTIID 2740

HSP 8 Score: 259.61 bits (662), Expect = 1.561e-80
Identity = 136/165 (82.42%), Postives = 148/165 (89.70%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFI LVQNKQYEKIDVDLINWLTTIIDTLRKV QVH KNEK+++ENLK ITETSNKYI+ L E  WE+L+KYIDES+RPEVI KI  FI  V+NK+YEKID  LIN++  I+
Sbjct: 3091 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFIALVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQIL 3255

HSP 9 Score: 208.379 bits (529), Expect = 1.501e-62
Identity = 109/165 (66.06%), Postives = 131/165 (79.39%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            +++I N    ++NLK ITE S +YI  L EL WE L+KYID  +RPEV+ KI TFI  VQNKQ+E+ID  L+NW+T II TLR+V+Q+HIKNE+VT EN K ITETSNKY+N+L +L WE L+KYIDE +RPE  KKI TFITLVQNKQYEKIDVDLINWLT II
Sbjct: 2422 EINITNTNNKQQNLKVITELSIQYIQLLNELKWEELIKYIDAKNRPEVVDKIKTFIKFVQNKQFEQIDTGLLNWITRIITTLREVKQIHIKNEEVTTENKKIITETSNKYVNYLIDLKWEELIKYIDEKNRPEFTKKIKTFITLVQNKQYEKIDVDLINWLTEII 2586

HSP 10 Score: 204.527 bits (519), Expect = 2.990e-61
Identity = 111/166 (66.87%), Postives = 134/166 (80.72%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            +V IKN K  E NLK I+ETSNK+I+ L E+NWE LVKYID+S+R EVI KI TFIT V+NKQYEKID +LINW+  II   R+V++VHIKNEK+T +N K IT+TSNKY+NFL ELNWE LVK I+E+ RPEV KKI TFITLVQNKQYEKID +L+N +  II+
Sbjct: 2165 EVQIKNTKTEEVNLKEISETSNKFIDILVEINWEELVKYIDQSNRLEVINKIKTFITFVKNKQYEKIDTNLINWINHIITIFRQVKEVHIKNEKITNDNRKLITDTSNKYVNFLIELNWEELVKQIEETKRPEVTKKIKTFITLVQNKQYEKIDSELLNLIVEIIN 2330

HSP 11 Score: 197.208 bits (500), Expect = 1.186e-58
Identity = 102/165 (61.82%), Postives = 131/165 (79.39%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            QVH KNEK+++ENLK ITETSNKYI+ L E  WE+L+KYIDES+RPEVI KI  FI  V+NK+YEKID  LIN++  I+ TL++VR +HIKNEK+ +EN+  I +TS KYI  L EL WE L++YIDE+SRPE+IKKI  FI+ V+NK Y+ IDVDL+N++  II
Sbjct: 3177 QVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHIKNEKIKKENVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQII 3341

HSP 12 Score: 179.489 bits (454), Expect = 1.780e-52
Identity = 97/165 (58.79%), Postives = 126/165 (76.36%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            +VHIKNEK+T +N K IT+TSNKY+NFL ELNWE LVK I+E+ RPEV KKI TFITLVQNKQYEKID +L+N +  II+TLR+V+ + I+NEK      + + + ++ Y+  L E+ WE L+KYIDE SRPE+IKKI  F+ L QNK+Y++ID  L+NWL  II
Sbjct: 2251 EVHIKNEKITNDNRKLITDTSNKYVNFLIELNWEELVKQIEETKRPEVTKKIKTFITLVQNKQYEKIDSELLNLIVEIINTLREVK-IKIENEKSNLSYRERVIKLTDSYLEILNEVKWEELMKYIDEQSRPEIIKKIKNFVILAQNKKYDEIDSTLLNWLNQII 2414

HSP 13 Score: 175.637 bits (444), Expect = 3.442e-51
Identity = 105/159 (66.04%), Postives = 127/159 (79.87%), Query Frame = 2 
Query:   29 TEENL---KTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            TEEN+   KTIT+ SN ++  L EL WE L+KYIDESSRPEV+ KI TF+TLV+NKQYEKID+DLI W+T II TL+K ++V IKN K  E NLK I+ETSNK+I+ L E+NWE LVKYID+S+R EVI KI TFIT V+NKQYEKID +LINW+  II
Sbjct: 2085 TEENIIDIKTITDKSNNFLITLTELKWEELLKYIDESSRPEVLNKIKTFVTLVENKQYEKIDIDLITWITEIITTLKKAKEVQIKNTKTEEVNLKEISETSNKFIDILVEINWEELVKYIDQSNRLEVINKIKTFITFVKNKQYEKIDTNLINWINHII 2243

HSP 14 Score: 163.31 bits (412), Expect = 7.802e-47
Identity = 84/165 (50.91%), Postives = 116/165 (70.30%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            ++ I+NEK      + + + ++ Y+  L E+ WE L+KYIDE SRPE+IKKI  F+ L QNK+Y++ID  L+NWL  II  LR++R+++I N    ++NLK ITE S +YI  L EL WE L+KYID  +RPEV+ KI TFI  VQNKQ+E+ID  L+NW+T II
Sbjct: 2336 KIKIENEKSNLSYRERVIKLTDSYLEILNEVKWEELMKYIDEQSRPEIIKKIKNFVILAQNKKYDEIDSTLLNWLNQIIVMLRRIREINITNTNNKQQNLKVITELSIQYIQLLNELKWEELIKYIDAKNRPEVVDKIKTFIKFVQNKQFEQIDTGLLNWITRII 2500

HSP 15 Score: 108.997 bits (271), Expect = 7.878e-28
Identity = 67/164 (40.85%), Postives = 97/164 (59.15%), Query Frame = 2 
Query:    5 VHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            +HIKNEK+ +EN+  I +TS KYI  L EL WE L++YIDE+SRPE+IKKI  FI+ V+NK Y+ IDVDL+N++  II TL+K+R     + +        I              NW  L++ I        I +I   ++++++K+Y+KID D I  L  II
Sbjct: 3264 IHIKNEKIKKENVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRKDIIKKIQIKLPG----------NWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWIEELQKII 3417

HSP 16 Score: 90.1225 bits (222), Expect = 3.004e-21
Identity = 60/129 (46.51%), Postives = 83/129 (64.34%), Query Frame = 2 
Query:   95 NWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEEN---LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDL 472
            NW  ++       R + I +I T I LV+  + +KID   + WL   ++ + KV+++       TEEN   +KTIT+ SN ++  L EL WE L+KYIDESSRPEV+ KI TF+TLV+NKQYEKID+DL
Sbjct: 2030 NWAKIINETKNIDRVKWIDRINTIIKLVKLNEVDKID---LTWLKE-LEKIFKVKEIP-----STEENIIDIKTITDKSNNFLITLTELKWEELLKYIDESSRPEVLNKIKTFVTLVENKQYEKIDIDL 2149

HSP 17 Score: 70.8626 bits (172), Expect = 1.427e-14
Identity = 45/135 (33.33%), Postives = 76/135 (56.30%), Query Frame = 2 
Query:   68 KYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDL 472
            +Y+  L+E NW  L+K++D+  R  +I  I   I +V  K+Y++ID + I  L  I+  +RK++    +N K+    L  IT    + +N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L
Sbjct:  436 RYVKLLKEWNWPELMKFVDQKERNNLIIGIEKMIGIVDQKRYKEIDNNFIQKLRFIVRFMRKIK---YRNNKIKPLTLNIITRNVKRLVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENL 567

HSP 18 Score: 62.003 bits (149), Expect = 1.457e-11
Identity = 41/133 (30.83%), Postives = 77/133 (57.89%), Query Frame = 2 
Query:   95 NWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTI 493
            NW  L++ I        I +I   ++++++K+Y+KID D I  L  II  + +  ++ +K E +  E   +I +  +  +  L+E  WE  + YIDES+R +V K +   I++V+++Q+EK+++ L+NW   I
Sbjct: 3370 NWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWIEELQKIISLISE-EEIKLKKEVILNEG--SIAKLKSN-VKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQI 3498

HSP 19 Score: 51.6026 bits (122), Expect = 5.849e-8
Identity = 52/199 (26.13%), Postives = 86/199 (43.22%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKV-----------------------------------------------------RQVHIKNEKVTEE--NLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDL 472
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK+                                                     R + I  +K T+    +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L
Sbjct: 1310 IKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENL 1508

HSP 20 Score: 49.2914 bits (116), Expect = 4.063e-7
Identity = 33/103 (32.04%), Postives = 55/103 (53.40%), Query Frame = 2 
Query:   83 LRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESS 391
            L+E  WE  + YIDES+R +V K +   I++V+++Q+EK+++ L+NW   I     K+    I N  V +   KT  +  N  +N    +     + Y+DES 
Sbjct: 3448 LQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDA-ITNGYVFDSAKKTWVK--NIVVNSRMRMKRSAGLNYMDESQ 3547

HSP 21 Score: 48.9062 bits (115), Expect = 4.425e-7
Identity = 47/156 (30.13%), Postives = 81/156 (51.92%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDVDLINWLTTIID 499
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+  +     II+
Sbjct: 1771 IKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKKPIIDIKVQKERLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNKIIN 1918

HSP 22 Score: 48.521 bits (114), Expect = 6.109e-7
Identity = 45/145 (31.03%), Postives = 77/145 (53.10%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDV 466
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+
Sbjct:  830 IKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKRPIIDIKVQKERLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDL 966

HSP 23 Score: 48.521 bits (114), Expect = 6.109e-7
Identity = 45/145 (31.03%), Postives = 77/145 (53.10%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDV 466
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+
Sbjct: 1150 IKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKRPIIDIKVQKERLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDL 1286

HSP 24 Score: 48.521 bits (114), Expect = 6.467e-7
Identity = 45/145 (31.03%), Postives = 77/145 (53.10%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDV 466
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+
Sbjct:  990 IKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKKPIIDIKVQKERLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDL 1126

HSP 25 Score: 48.521 bits (114), Expect = 6.467e-7
Identity = 45/145 (31.03%), Postives = 77/145 (53.10%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDV 466
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+
Sbjct: 1451 IKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKKPIIDIKVQKERLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDL 1587

HSP 26 Score: 47.7506 bits (112), Expect = 1.069e-6
Identity = 48/169 (28.40%), Postives = 84/169 (49.70%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDVDLINWLTTIID 499
            ++  +N K+    L  IT    + +N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+  +     II+
Sbjct:  497 KIKYRNNKIKPLTLNIITRNVKRLVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKRPIIDIKVQKERLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIIN 657

HSP 27 Score: 46.595 bits (109), Expect = 3.458e-6
Identity = 45/145 (31.03%), Postives = 77/145 (53.10%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDV 466
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+
Sbjct: 1611 IKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKRPIIDIKVQKEKLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDL 1747
[Close]
BLAST of SMED30034869 vs. Planmine SMEST
Match: SMESG000008835.1 (SMESG000008835.1)

HSP 1 Score: 185.267 bits (469), Expect = 1.862e-54
Identity = 101/164 (61.59%), Postives = 123/164 (75.00%), Query Frame = 2 
Query:    8 HIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            H+ NEK    NLKTITETS KYIN L EL WE L KYIDE +R  VI KI TFI  V+NK++E ID+DL+NW+  II TLRK R+++  NEKV  +NLKT+TETS KY N+L EL WE LV++ID SSR EVI KI TFI+ VQNK ++KIDV+L NW+  II+
Sbjct: 2195 HVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRKAREINETNEKVKIKNLKTLTETSLKYFNYLIELKWEELVQHIDSSSRVEVINKIKTFISFVQNKSFDKIDVNLANWIAEIIN 2358

HSP 2 Score: 183.726 bits (465), Expect = 5.048e-54
Identity = 100/163 (61.35%), Postives = 117/163 (71.78%), Query Frame = 2 
Query:    8 HIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            H+ NEK    NLKTITETS KYIN L EL WE L KYIDE +R  VI KI TFI  V+NK++E ID+DL+NW+  II TLR  R+ H+ NEK    NLKTITETS KYIN L EL WE L KYIDE +R  VI KI TFI  V+NK++E ID+DL+NW+  II
Sbjct: 2023 HVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRNARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEII 2185

HSP 3 Score: 183.726 bits (465), Expect = 5.048e-54
Identity = 100/163 (61.35%), Postives = 117/163 (71.78%), Query Frame = 2 
Query:    8 HIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            H+ NEK    NLKTITETS KYIN L EL WE L KYIDE +R  VI KI TFI  V+NK++E ID+DL+NW+  II TLR  R+ H+ NEK    NLKTITETS KYIN L EL WE L KYIDE +R  VI KI TFI  V+NK++E ID+DL+NW+  II
Sbjct: 2109 HVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRNARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEII 2271

HSP 4 Score: 170.629 bits (431), Expect = 2.389e-49
Identity = 91/154 (59.09%), Postives = 113/154 (73.38%), Query Frame = 2 
Query:   35 ENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            +N+K+IT+ S K+I+ L EL WE L KYIDE +R  VI KI TFI  V+NK++E ID+DL+NW+  II TLRK R+ H+ NEK    NLKTITETS KYIN L EL WE L KYIDE +R  VI KI TFI  V+NK++E ID+DL+NW+  II
Sbjct: 1946 KNIKSITDKSLKFIDSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRKARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEII 2099

HSP 5 Score: 135.961 bits (341), Expect = 2.485e-37
Identity = 77/153 (50.33%), Postives = 107/153 (69.93%), Query Frame = 2 
Query:   38 NLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            +L  +TE S   I  L +LNWE LVKYI E ++PEV+ KI TFI  V+NK+++ ID + IN++T II T +K+++  IK E    +N+K+IT+ S K+I+ L EL WE L KYIDE +R  VI KI TFI  V+NK++E ID+DL+NW+  II
Sbjct: 1863 DLAVVTEKSISLITDLNQLNWEELVKYIGEPNQPEVVNKIKTFIFNVKNKKFQNIDGETINFITEIISTFKKIKENRIKTE--NTKNIKSITDKSLKFIDSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEII 2013

HSP 6 Score: 114.005 bits (284), Expect = 1.294e-29
Identity = 74/161 (45.96%), Postives = 101/161 (62.73%), Query Frame = 2 
Query:   17 NEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            NEKV  +NLKT+TETS KY N+L EL WE LV++ID SSR EVI KI TFI+ VQNK ++KIDV+L NW+  II+ LRK+R   I   KVTE     ITE     I      NW+ L++ I+         +I   +T++++K ++KID + I  L  I++
Sbjct: 2284 NEKVKIKNLKTLTETSLKYFNYLIELKWEELVQHIDSSSRVEVINKIKTFISFVQNKSFDKIDVNLANWIAEIINILRKIR---IGQSKVTENKTLKITEN----IQLTLPSNWQELLQRINGIDYQLWTSRIDQLLTIMKSKNHQKIDFEWIGELEKIVN 2437

HSP 7 Score: 108.612 bits (270), Expect = 1.013e-27
Identity = 62/167 (37.13%), Postives = 109/167 (65.27%), Query Frame = 2 
Query:    2 QVHIKNE-KVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            ++H K+  K+  +  K I     K +N+L + NW+ L+KY+ ++ + + + KI   I+L++NK Y+ ID DL+ W++TI   L+ VR++  K  K  +ENLK + + S+K+++ L +L W  ++  I+  ++PE+ +KI  FITLVQN++Y+KID + +NWL  II+
Sbjct: 3163 KIHQKHHRKIVSKERKRIVHKVIKRVNWLEKWNWKNLMKYVVDNHKRDTLVKIKYIISLIKNKDYDNIDEDLLKWISTIKTKLKIVRKIK-KETKNIDENLKIVMKKSHKFVDILNQLRWIDIIDEIEGDNKPEIKEKIKRFITLVQNREYDKIDTEFLNWLFEIIE 3328

HSP 8 Score: 83.5741 bits (205), Expect = 4.355e-19
Identity = 54/156 (34.62%), Postives = 95/156 (60.90%), Query Frame = 2 
Query:   32 EENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTE-ENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            +ENLK + + S+K+++ L +L W  ++  I+  ++PE+ +KI  FITLVQN++Y+KID + +NWL  II+ ++K +Q      K+TE +N K +       IN     NW+ L++ ++         +I   ++LVQ+K+Y+KID + I+ L  I+
Sbjct: 3259 DENLKIVMKKSHKFVDILNQLRWIDIIDEIEGDNKPEIKEKIKRFITLVQNREYDKIDTEFLNWLFEIIENIKKTKQ------KLTETDNTKNVKVIEKIQINL--PSNWKELLQNVEGIDVNLWTARIENLLSLVQSKEYQKIDFEWIDELQKIV 3406

HSP 9 Score: 67.781 bits (164), Expect = 1.531e-13
Identity = 56/213 (26.29%), Postives = 92/213 (43.19%), Query Frame = 2 
Query:   23 KVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQV----HIKNEKVTEENLKTITETSNKY--------------------------------------------------INFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            KV E  +KT+ E     +N L++ NWE  +KYIDE  R EV + +   IT+++N +Y+K+DV  ++W   I +   K+       ++ +EK T+  +KT+ E  N                                                    IN L+  NWE  ++YIDE  R  +   I   I +++N + +K+D D I+W   I +
Sbjct: 2885 KVNEVTIKTVKEN----VNSLKQWNWETYLKYIDEDKREEVQRNVNNVITIIENVEYDKLDVKSVDWFNQIFEVFNKMYYATTNGYVYDEK-TKSWVKTVVEVENNTRKRRCILLNLLHGAAKKLIPHISIGLNIGGKPRRPRPNYNQVSIQTIRTNINSLKLWNWENFLQYIDEDKREPIQTNINKIINVIENGESDKLDPDTIDWFIQIFE 3092

HSP 10 Score: 65.0846 bits (157), Expect = 1.171e-12
Identity = 44/115 (38.26%), Postives = 69/115 (60.00%), Query Frame = 2 
Query:  152 KITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            +I T   L+ +KQ +KID    +WL  +++      +    + ++   +L  +TE S   I  L +LNWE LVKYI E ++PEV+ KI TFI  V+NK+++ ID + IN++T II
Sbjct: 1821 RIKTITELINSKQTDKIDY---SWLLELLEIFNFPDE---PDREIPTIDLAVVTEKSISLITDLNQLNWEELVKYIGEPNQPEVVNKIKTFIFNVKNKKFQNIDGETINFITEII 1929

HSP 11 Score: 65.0846 bits (157), Expect = 1.194e-12
Identity = 37/118 (31.36%), Postives = 69/118 (58.47%), Query Frame = 2 
Query:  146 IKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            IK+I   + +++  +++KID   I+ L  ++D + +     ++  KV E  +KT+ E     +N L++ NWE  +KYIDE  R EV + +   IT+++N +Y+K+DV  ++W   I +
Sbjct: 2840 IKRIQLLLPMIELNEFDKIDFGWIDELQKLVDLITEESIKTVEKPKVNEVTIKTVKEN----VNSLKQWNWETYLKYIDEDKREEVQRNVNNVITIIENVEYDKLDVKSVDWFNQIFE 2953

HSP 12 Score: 63.5438 bits (153), Expect = 5.176e-12
Identity = 45/140 (32.14%), Postives = 80/140 (57.14%), Query Frame = 2 
Query:   95 NWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKY-----INFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            NW+ L++ ++         +I   ++LVQ+K+Y+KID +   W    ID L+K+  V + + +VTE   K +   S K      IN L++ NWE L+KYI+E++R +V   +   I +V+N++++KID   + W   I++
Sbjct: 3359 NWKELLQNVEGIDVNLWTARIENLLSLVQSKEYQKIDFE---W----IDELQKI--VQLISTQVTESKEKFVLPESTKTKIKENINSLKQWNWESLLKYIEETNRKQVETDVKKMIVIVENEEFDKIDSQFVQWFNQIVE 3489

HSP 13 Score: 62.7734 bits (151), Expect = 7.309e-12
Identity = 50/190 (26.32%), Postives = 79/190 (41.58%), Query Frame = 2 
Query:   74 INFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKT-------------------------------------------------ITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTII 496
            IN L+  NWE  ++YIDE  R  +   I   I +++N + +K+D D I+W   I +  +K+    I+N  V + N +T                                                 I + S+KY+  L EL W  L+  ID  ++  +  KI  FI  V  ++Y KI+   +NWL+ II
Sbjct: 2603 INSLKLWNWENFLQYIDEDKREPIQTNINKIIYVIENGESDKLDPDTIDWFIQIFEEFKKI-NYAIENGYVYDPNTRTWTKNTVEVENKIRKKRCLFNIFKGFHRLLPRISIRGRIGHVNREDKLNFIIDMSHKYLVILNELRWNELIDSIDGFNKRSIFVKINKFIHYVNTRKYHKINRKFLNWLSQII 2791

HSP 14 Score: 59.3066 bits (142), Expect = 1.463e-10
Identity = 45/166 (27.11%), Postives = 86/166 (51.81%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            ++ I   KVTE     ITE     I      NW+ L++ I+         +I   +T++++K ++KID + I  L  I++ + +   + +K E    E   T  E    +++ L+E NW  L+ YIDE++R ++  +I   I++V++  +EK+++  +NW   I +
Sbjct: 2362 KIRIGQSKVTENKTLKITEN----IQLTLPSNWQELLQRINGIDYQLWTSRIDQLLTIMKSKNHQKIDFEWIGELEKIVNLISQ-ESIKMKKEVFLNE---TSIEKLKGFVSQLKEWNWNSLLIYIDENNRQQIQNEIQGLISVVESGNFEKLEIQTVNWFYQIFE 2519

HSP 15 Score: 55.0694 bits (131), Expect = 3.487e-9
Identity = 49/164 (29.88%), Postives = 80/164 (48.78%), Query Frame = 2 
Query:   32 EENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLR--KVRQVHIKNEKVTEENLKTITETSNKYINFLRELNW-EVLVKYIDESSRPEV-----IKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            E+ L  I + S+KY+  L EL W  L+  ID  ++  +  KI  FI  V  ++Y KI+   +NWL+ II TL+  K+R      +K  E   K +    +         NW EV+VK       P +     IK+I   + +++  +++KID   I+ L  ++D
Sbjct: 2723 EDKLNFIIDMSHKYLVILNELRWNELIDSIDGFNKRSIFVKINKFIHYVNTRKYHKINRKFLNWLSQIIITLQNTKIRLTGSMKQKRIEIAKKVLVNLPS---------NWKEVIVKL------PNINVDLWIKRIQLLLPMIELNEFDKIDFGWIDELQKLVD 2871

HSP 16 Score: 50.447 bits (119), Expect = 1.416e-7
Identity = 22/61 (36.07%), Postives = 40/61 (65.57%), Query Frame = 2 
Query:   74 INFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKV 256
            IN L++ NWE L+KYI+E++R +V   +   I +V+N++++KID   + W   I++   K+
Sbjct: 3434 INSLKQWNWESLLKYIEETNRKQVETDVKKMIVIVENEEFDKIDSQFVQWFNQIVELFNKI 3494

HSP 17 Score: 49.6766 bits (117), Expect = 2.832e-7
Identity = 29/97 (29.90%), Postives = 56/97 (57.73%), Query Frame = 2 
Query:   47 TITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKT----ITETSN 325
            T  E    +++ L+E NW  L+ YIDE++R ++  +I   I++V++  +EK+++  +NW   I +T  K+++  + N  V +  +KT    IT+  N
Sbjct: 2455 TSIEKLKGFVSQLKEWNWNSLLIYIDENNRQQIQNEIQGLISVVESGNFEKLEIQTVNWFYQIFETFSKIKEF-MNNGYVYDSKIKTWVKHITQNQN 2550

HSP 18 Score: 48.9062 bits (115), Expect = 4.728e-7
Identity = 47/156 (30.13%), Postives = 81/156 (51.92%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDVDLINWLTTIID 499
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+  +     II+
Sbjct: 1063 IKVITKDVKKFVNRLIKWNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKRPIIDIKVQKERLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIIN 1210

HSP 19 Score: 48.9062 bits (115), Expect = 4.728e-7
Identity = 47/156 (30.13%), Postives = 81/156 (51.92%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDVDLINWLTTIID 499
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+  +     II+
Sbjct: 1543 IKVITKDVKKFVNRLIKWNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKRPIIDIKVQKERLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIIN 1690

HSP 20 Score: 46.9802 bits (110), Expect = 2.114e-6
Identity = 32/149 (21.48%), Postives = 75/149 (50.34%), Query Frame = 2 
Query:   71 YINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLREL------NWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIID 499
            ++ +L++  W  L+K++  + +  V K +     +++ +++EKI+++L+  +  ++  L+++ ++HIK               S K  N +R L      +W  ++  ++   R + I++      L+Q K+Y +IDV+ +     IID
Sbjct:  279 HVEWLKKWKWMELLKFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVKNVKFVMRLLKRLWRIHIK-------------YGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNEIDVNWLAEFKPIID 414

HSP 21 Score: 46.595 bits (109), Expect = 2.972e-6
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 2 
Query:   74 INFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETS 322
            IN L+  NWE  ++YIDE  R  +   I   I +++N + +K+D D I+W   I +  +K+    I+N  V + N +T T+ +
Sbjct: 3037 INSLKLWNWENFLQYIDEDKREPIQTNINKIINVIENGESDKLDPDTIDWFIQIFEEFKKI-NYAIENGYVYDPNTRTWTKNT 3118

HSP 22 Score: 45.8246 bits (107), Expect = 5.444e-6
Identity = 46/156 (29.49%), Postives = 80/156 (51.28%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDVDLINWLTTIID 499
            +K IT+   ++I  L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+  +     II+
Sbjct:  743 VKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKKPIIDIKVQKERLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIIN 890

HSP 23 Score: 45.8246 bits (107), Expect = 5.444e-6
Identity = 46/156 (29.49%), Postives = 80/156 (51.28%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDVDLINWLTTIID 499
            +K IT+   ++I  L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+  +     II+
Sbjct: 1223 VKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKKPIIDIKVQKERLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIIN 1370

HSP 24 Score: 45.4394 bits (106), Expect = 7.437e-6
Identity = 44/145 (30.34%), Postives = 76/145 (52.41%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDV 466
            +K IT+   ++I  L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+
Sbjct:  903 VKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKKPIIDIKVQKERLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDL 1039

HSP 25 Score: 45.4394 bits (106), Expect = 7.437e-6
Identity = 44/145 (30.34%), Postives = 76/145 (52.41%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDV 466
            +K IT+   ++I  L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+
Sbjct: 1383 VKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKKPIIDIKVQKERLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDL 1519

HSP 26 Score: 45.0542 bits (105), Expect = 9.155e-6
Identity = 44/147 (29.93%), Postives = 76/147 (51.70%), Query Frame = 2 
Query:   68 KYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK---KITTFITLVQNKQYEKIDVDLINWLTTIID 499
            K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK     IKN+K    ++K   E     +  + + NW+   K I+E+   + +K   +I   I L++  Q +KID+  +     II+
Sbjct:  592 KFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKRPIIDIKVQKERLLNKLKLIIDTNWQ---KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIIN 730
The following BLAST results are available for this feature:
BLAST of SMED30034869 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034869 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034869 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034869 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034869 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034869 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034869 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034869 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A1S6KMJ11.861e-5966.87Uncharacterized protein (Fragment) OS=Schmidtea me... [more]
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BLAST of SMED30034869 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034869 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034869 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034869 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034869 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034869 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000008843.13.959e-100100.00SMESG000008843.1[more]
SMESG000008835.11.862e-5461.59SMESG000008835.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034869 ID=SMED30034869|Name=SMED30034869|organism=Schmidtea mediterranea sexual|type=transcript|length=501bp
GCAGGTTCACATTAAAAATGAAAAAGTTACCGAAGAAAATTTGAAAACAA
TAACTGAGACATCAAATAAATACATCAATTTCTTGAGAGAACTGAATTGG
GAAGTACTAGTGAAATATATAGATGAAAGCAGTCGGCCAGAAGTGATCAA
GAAAATAACAACTTTTATCACTCTTGTTCAAAATAAACAATATGAAAAGA
TAGATGTTGATCTAATAAATTGGTTAACGACAATTATAGATACATTGAGA
AAAGTAAGGCAGGTTCACATTAAAAATGAAAAAGTTACCGAAGAAAATTT
GAAAACAATAACTGAGACATCAAATAAATACATCAATTTCTTGAGAGAAC
TGAATTGGGAAGTACTAGTGAAATATATAGATGAAAGCAGTCGGCCAGAA
GTGATCAAGAAAATAACAACTTTTATCACTCTTGTTCAAAATAAACAATA
TGAAAAGATAGATGTTGATCTAATAAATTGGTTAACGACAATTATAGATA
C
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protein sequence of SMED30034869-orf-1

>SMED30034869-orf-1 ID=SMED30034869-orf-1|Name=SMED30034869-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=166bp
QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK
KITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENL
KTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQY
EKIDVDLINWLTTIID
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region