Tetraspanin

Overview
NameTetraspanin
Smed IDSMED30034859
Length (bp)1275
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Tetraspanin (SMED30034859) t-SNE clustered cells

Violin plots show distribution of expression levels for Tetraspanin (SMED30034859) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Tetraspanin (SMED30034859) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Tetraspanin (SMED30034859) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30034859

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30034859SMESG000056555.1 SmedASXL_008228SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30034859SMESG000056555.1 dd_Smed_v4_3116_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30034859SMESG000056555.1 dd_Smed_v4_3116_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30034859SMESG000056555.1 dd_Smed_v4_3116_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30034859SMESG000056555.1 dd_Smed_v4_3116_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30034859SMESG000056555.1 dd_Smed_v4_3116_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30034859SMESG000056555.1 dd_Smed_v4_3116_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30034859SMESG000056555.1 dd_Smed_v6_3116_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30034859SMESG000056555.1 OX_Smed_1.0.17237ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30034859SMESG000056555.1 dd_Smed_v6_3116_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Tetraspanin vs. TrEMBL
Match: A0A1I8I249 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 7.877e-12
Identity = 48/158 (30.38%), Postives = 80/158 (50.63%), Query Frame = 2
Query:  614 KSIVEGILKDMIIKYEGFFSANPESFLINVMNIGLNCCGAINYQDFNA-SVKFNRTYSGTFTYGGKTETYNVQLNYPISCCQFKEFQFV-NSRECIENKGSNNKDKGCVTSLFDSIQNYTTM--TIQIICGIIGYQLLILLAASVCLVSLIKEAGVKP 1075
            KS V+  ++  + K+ G  S N ++ L N++ I + CCG  N  DF   S K+NRTYS +      +  Y   L YP++CC+F    F+     C  N  +++  +GC  ++FD I+ Y  +      + GI+GY+ L++  A +   +      VKP
Sbjct:    2 KSSVKSEMEQSLKKFTGISSKNIQTLLQNLLQIFVRCCGVNNGTDFETMSPKWNRTYSMS----ESSFNYTFNLTYPVTCCKFNLTSFLPEDANCPLNNTNSHYRRGCFEAMFDFIEQYMKLNAVFYALGGILGYEALLIAGAIIIHRASDDSNKVKP 155          
BLAST of Tetraspanin vs. TrEMBL
Match: A0A1I8I7S1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 9.270e-12
Identity = 60/201 (29.85%), Postives = 102/201 (50.75%), Query Frame = 2
Query:  497 CSVCCKARILLIVYITXXXXXXXXXXXXXXXXXXDPTKAKSIVEGILKDMIIKYEGFFSANPESFLINVMNIGLNCCGAINYQDFNA-SVKFNRTYSGTFTYGGKTETYNVQLNYPISCCQFKEFQFVNSRE-CIENKG---SNNKDKGCVTSLFDSIQNYTTMTIQI--ICGIIGYQLLILLAASVCLVSLIKEAGVKPI 1078
            C  CC  R  LI+Y  ++ ++++ Q+IVI I+ +     K      +++ + K+ G  S N +S L N++++ + CCG  N  DF   S  +NRTYS           Y+  L YP++CC+F    F    E C  N     ++N  KGC  ++F  I +Y  +      + G++G+Q L+++   + LV   K+ G KP+
Sbjct:  507 CGACCTVRGCLIIYAVMVGIMLIGQVIVISIWFSSNNLFKENARSAMEESLKKFTGISSKNIQSLLQNLVHVFVGCCGVTNGTDFQTMSPDWNRTYS--------VSGYSFNLTYPLTCCKFDLSTFKPKDEYCPYNNTTPETSNYKKGCYEAIFTFIDDYLPLDYLFYGLGGVLGFQCLLIVGVVILLVHDQKDNG-KPM 698          
BLAST of Tetraspanin vs. TrEMBL
Match: A0A1I8IBF5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.470e-11
Identity = 61/201 (30.35%), Postives = 102/201 (50.75%), Query Frame = 2
Query:  497 CSVCCKARILLIVYITXXXXXXXXXXXXXXXXXXDPTKAKSIVEGILKDMIIKYEGFFSANPESFLINVMNIGLNCCGAINYQDFNA-SVKFNRTYSGTFTYGGKTETYNVQLNYPISCCQFKEFQFVNSRE-CIENKG---SNNKDKGCVTSLFDSIQNYTTMTIQI--ICGIIGYQLLILLAASVCLVSLIKEAG-VKP 1075
            C  CC  R  LI+Y  ++ ++++ Q+IVI I+ +     K      +++ + K+ G  S N +S L N++++ + CCG  N  DF   S  +NRTYS           Y+  L YP++CC+F    F    E C  N     ++N  KGC  ++F  I +Y  +      + G++G+Q L+++   + LV   K+ G VKP
Sbjct:   80 CGACCTVRGCLIIYAVMVGIMLIGQVIVISIWFSSNNLFKENARSAMEESLKKFTGISSKNIQSLLQNLVHVFVGCCGVTNGTDFQTMSPDWNRTYS--------VSGYSFNLTYPLTCCKFDLSTFKPKDEYCPYNNTTPETSNYKKGCYEAIFTFIDDYLPLDYLFYGLGGVLGFQCLLIVGVVILLVHDQKDNGKVKP 272          
BLAST of Tetraspanin vs. TrEMBL
Match: A0A1I8HMV2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006782g1 PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 3.068e-10
Identity = 61/201 (30.35%), Postives = 102/201 (50.75%), Query Frame = 2
Query:  497 CSVCCKARILLIVYITXXXXXXXXXXXXXXXXXXDPTKAKSIVEGILKDMIIKYEGFFSANPESFLINVMNIGLNCCGAINYQDFNA-SVKFNRTYSGTFTYGGKTETYNVQLNYPISCCQFKEFQFVNSRE-CIENKG---SNNKDKGCVTSLFDSIQNYTTMTIQI--ICGIIGYQLLILLAASVCLVSLIKEAG-VKP 1075
            C  CC  R  LI+Y  ++ ++++ Q+IVI I+ +     K      +++ + K+ G  S N +S L N++++ + CCG  N  DF   S  +NRTYS           Y+  L YP++CC+F    F    E C  N     ++N  KGC  ++F  I +Y  +      + G++G+Q L+++   + LV   K+ G VKP
Sbjct:  107 CGACCTVRGCLIIYAVMVGIMLIGQVIVISIWFSSNNLFKENARSAMEESLKKFTGISSKNIQSLLQNLVHVFVGCCGVTNGTDFQTMSPDWNRTYS--------VSGYSFNLTYPLTCCKFDLSTFKPKDEYCPYNNTTPETSNYKKGCYEAIFTFIDDYLPLDYLFYGLGGVLGFQCLLIVGVVILLVHDQKDNGKVKP 299          
BLAST of Tetraspanin vs. TrEMBL
Match: A0A267DLD0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006782g2 PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 3.214e-10
Identity = 61/201 (30.35%), Postives = 102/201 (50.75%), Query Frame = 2
Query:  497 CSVCCKARILLIVYITXXXXXXXXXXXXXXXXXXDPTKAKSIVEGILKDMIIKYEGFFSANPESFLINVMNIGLNCCGAINYQDFNA-SVKFNRTYSGTFTYGGKTETYNVQLNYPISCCQFKEFQFVNSRE-CIENKG---SNNKDKGCVTSLFDSIQNYTTMTIQI--ICGIIGYQLLILLAASVCLVSLIKEAG-VKP 1075
            C  CC  R  LI+Y  ++ ++++ Q+IVI I+ +     K      +++ + K+ G  S N +S L N++++ + CCG  N  DF   S  +NRTYS           Y+  L YP++CC+F    F    E C  N     ++N  KGC  ++F  I +Y  +      + G++G+Q L+++   + LV   K+ G VKP
Sbjct:  107 CGACCTVRGCLIIYAVMVGIMLIGQVIVISIWFSSNNLFKENARSAMEESLKKFTGISSKNIQSLLQNLVHVFVGCCGVTNGTDFQTMSPDWNRTYS--------VSGYSFNLTYPLTCCKFDLSTFKPKDEYCPYNNTTPETSNYKKGCYEAIFTFIDDYLPLDYLFYGLGGVLGFQCLLIVGVVILLVHDQKDNGKVKP 299          
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000056555.1 (SMESG000056555.1)

HSP 1 Score: 376.326 bits (965), Expect = 3.628e-130
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 2
Query:  302 TAAKDFVVDXXXXXXXXXXXXXXXXXXXXVNGAQMFTYXXXXXXXXXXXXXXXXXXXXXXXXXXMCSVCCKARILLIVYITXXXXXXXXXXXXXXXXXXDPTKAKSIVEGILKDMIIKYEGFFSANPESFLINVMNIGLNCCGAINYQDFNASVKFNRTYSGTFTYGGKTETYNVQLNYPISCCQFKEFQFVNSRECIENKGSNNKDKGCVTSLFDSIQNYTTMTIQIICGIIGYQLLILLAASVCLVSLIKEAGVKPI 1078
            TAAKDFVVDKLSPLLKSFFDSLSSNSNLNVNGAQMFTYLLNSLSSVAIALIIIGAIFFIISIIAMCSVCCKARILLIVYITILSVIVLAQIIVIIIFAADPTKAKSIVEGILKDMIIKYEGFFSANPESFLINVMNIGLNCCGAINYQDFNASVKFNRTYSGTFTYGGKTETYNVQLNYPISCCQFKEFQFVNSRECIENKGSNNKDKGCVTSLFDSIQNYTTMTIQIICGIIGYQLLILLAASVCLVSLIKEAGVKPI
Sbjct:   15 TAAKDFVVDKLSPLLKSFFDSLSSNSNLNVNGAQMFTYLLNSLSSVAIALIIIGAIFFIISIIAMCSVCCKARILLIVYITILSVIVLAQIIVIIIFAADPTKAKSIVEGILKDMIIKYEGFFSANPESFLINVMNIGLNCCGAINYQDFNASVKFNRTYSGTFTYGGKTETYNVQLNYPISCCQFKEFQFVNSRECIENKGSNNKDKGCVTSLFDSIQNYTTMTIQIICGIIGYQLLILLAASVCLVSLIKEAGVKPI 273          
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000056558.1 (SMESG000056558.1)

HSP 1 Score: 204.142 bits (518), Expect = 8.100e-63
Identity = 112/197 (56.85%), Postives = 151/197 (76.65%), Query Frame = 2
Query:  494 MCSVCCKARILLIVYITXXXXXXXXXXXXXXXXXXDPTKAKSIVEGILKDMIIKYEGFFSANPESFLINVMNIGLNCCGAINYQDFNASVKFNRTYSGTFTYGGKTETYNVQLNYPISCCQFKEFQFVNSRECIENKGSNNKDKGCVTSLFDSIQNYTTMTIQIICGIIGYQLLILLAASVCLVSLIKEAG--VKPI 1078
            MCSVCCK++ L+I+Y++I+S +   QIIV+II+AA+P  +K ++  +LK+MI KY GF +  PESFL+N +NI +NCCGA  YQDFN+S+ FNRT+SG FTYG K++TYNV L YPISCC+FK+FQFVN   C++N+  NNKD GC  SLF+SI+ Y     Q++ GIIGYQ LI++AA VCLVSL ++    VKP+
Sbjct:   79 MCSVCCKSQCLIIIYLSIISAVFAIQIIVVIIYAANPEMSKKMINDLLKNMIAKYRGFTNTEPESFLVNFLNIFMNCCGATGYQDFNSSINFNRTFSGNFTYGSKSQTYNVYLYYPISCCKFKDFQFVNMNGCLQNQTDNNKDSGCTESLFNSIKEYLIKVYQVVGGIIGYQSLIIIAAVVCLVSLKQDLAQNVKPV 275          
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000056558.1 (SMESG000056558.1)

HSP 1 Score: 204.527 bits (519), Expect = 1.126e-62
Identity = 112/197 (56.85%), Postives = 151/197 (76.65%), Query Frame = 2
Query:  494 MCSVCCKARILLIVYITXXXXXXXXXXXXXXXXXXDPTKAKSIVEGILKDMIIKYEGFFSANPESFLINVMNIGLNCCGAINYQDFNASVKFNRTYSGTFTYGGKTETYNVQLNYPISCCQFKEFQFVNSRECIENKGSNNKDKGCVTSLFDSIQNYTTMTIQIICGIIGYQLLILLAASVCLVSLIKEAG--VKPI 1078
            MCSVCCK++ L+I+Y++I+S +   QIIV+II+AA+P  +K ++  +LK+MI KY GF +  PESFL+N +NI +NCCGA  YQDFN+S+ FNRT+SG FTYG K++TYNV L YPISCC+FK+FQFVN   C++N+  NNKD GC  SLF+SI+ Y     Q++ GIIGYQ LI++AA VCLVSL ++    VKP+
Sbjct:   98 MCSVCCKSQCLIIIYLSIISAVFAIQIIVVIIYAANPEMSKKMINDLLKNMIAKYRGFTNTEPESFLVNFLNIFMNCCGATGYQDFNSSINFNRTFSGNFTYGSKSQTYNVYLYYPISCCKFKDFQFVNMNGCLQNQTDNNKDSGCTESLFNSIKEYLIKVYQVVGGIIGYQSLIIIAAVVCLVSLKQDLAQNVKPV 294          
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000041117.1 (SMESG000041117.1)

HSP 1 Score: 73.559 bits (179), Expect = 1.961e-14
Identity = 59/192 (30.73%), Postives = 91/192 (47.40%), Query Frame = 2
Query:  506 CCKARILLIVYITXXXXXXXXXXXXXXXXXXDPTK--AKSIVEGILKDMIIKYEGFFSANPESFLINVMNIGLNCCGAINYQDFNASVKFNRTYSGTFTYGGKTETYNVQLNYPISCCQFKE---FQFVNSRECIENKGSNNKDKGCVTSLFDSIQNYTTMTIQIICGIIGYQLLILLAASVCLVSLIKEAG 1066
            CC  +++LIV    L    LA I V+  + ++PT      +   I  D+I KY    S  P+S LINV+ I  NCCG  NY D+N +  F R  +        ++  N  + +P+SCC +K+       +   CI+ + S+  + GC T L   ++ Y           IG  + IL+AA +  + LIK A 
Sbjct:  102 CCTLKLILIVICIFLICFTLALIGVVAYYGSNPTDRILVPLTNTIQNDIISKYRDMNSTEPQSMLINVIQITANCCGVYNYTDYNNATNFYRQATA-------SDNKNYPIIFPLSCCDYKQDFTIDMASRDSCIKTQTSSKMNMGCYTRLEPDLRKYLGYCF----ATIGITIGILVAAIIVTIILIKCAA 282          
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000005510.1 (SMESG000005510.1)

HSP 1 Score: 68.5514 bits (166), Expect = 8.506e-13
Identity = 38/114 (33.33%), Postives = 59/114 (51.75%), Query Frame = 2
Query:  623 VEGILKDMIIKYEGFFSANPESFLINVMNIGLNCCGAINYQDFNASVKFNRTYSGTFTYGGKTETYNVQLNYPISCCQFKEFQFVNSRECIENKGSNNKDKGCVTSLFDSIQNY 964
            VE  L  +I KYEG+ + N ES +INV+    +CCG  NY DF+ + K+     G                +P+SCC+ +E ++VN  EC +   S +  KGC +S+   ++ Y
Sbjct:  143 VEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLG--------------FKFPLSCCESREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKY 242          
The following BLAST results are available for this feature:
BLAST of Tetraspanin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8I2497.877e-1230.38Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8I7S19.270e-1229.85Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8IBF59.470e-1130.35Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HMV23.068e-1030.35Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DLD03.214e-1030.35Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Tetraspanin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000056555.13.628e-130100.00SMESG000056555.1[more]
SMESG000056558.18.100e-6356.85SMESG000056558.1[more]
SMESG000056558.11.126e-6256.85SMESG000056558.1[more]
SMESG000041117.11.961e-1430.73SMESG000041117.1[more]
SMESG000005510.18.506e-1333.33SMESG000005510.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034859 ID=SMED30034859|Name=Tetraspanin|organism=Schmidtea mediterranea sexual|type=transcript|length=1275bp
TGATATTTAACCGTGTATTCATACAATCACAGTTTTTCTATTGTAATTGT
AACAATGTCAATAAAAAATGAATATAAAATGATAAAAATATTTTCAGTGA
TTTTGATTGGATGACATTCGCTCTGTTATTCGTATCATTTCTTAGCAACG
GAACTCTATAATTGTAGAATTATTTTGTTCAAATCATTTTCATCGACAGA
AAATGGGGCTATTCTATTGCTGTTGTTGCACACTTTTGATCCTGATTAAC
ATAATATATATGATAATCGGTATCGTTCTTCTAGTTCTTGGAATACTTTT
AACAGCAGCTAAAGATTTCGTTGTGGATAAACTCAGTCCACTTTTGAAAT
CATTTTTCGACAGCCTCTCTTCAAATTCAAATCTAAATGTCAACGGAGCA
CAAATGTTTACGTATTTGCTTAATTCATTAAGCTCGGTGGCCATTGCCTT
AATAATAATTGGAGCAATATTCTTTATAATATCTATAATTGCAATGTGCT
CAGTCTGCTGCAAAGCGAGAATTCTTCTTATTGTGTACATAACAATATTA
TCGGTCATAGTTTTGGCTCAAATTATTGTCATTATTATATTCGCCGCAGA
TCCAACAAAAGCTAAATCTATTGTTGAAGGCATACTAAAAGATATGATAA
TCAAATATGAAGGATTTTTTTCGGCAAATCCAGAAAGTTTCTTAATTAAT
GTTATGAACATAGGACTTAATTGTTGTGGTGCCATAAACTATCAAGATTT
TAATGCATCTGTAAAATTTAATAGAACTTATTCTGGAACATTTACATATG
GAGGAAAAACTGAAACATATAATGTTCAACTTAATTATCCAATATCTTGT
TGCCAGTTCAAAGAATTCCAATTTGTCAACTCTCGGGAGTGCATAGAGAA
CAAAGGATCCAATAATAAAGACAAGGGTTGCGTTACAAGTTTATTTGATA
GTATTCAAAACTACACTACAATGACTATTCAAATAATTTGTGGCATAATT
GGCTATCAATTACTGATACTTCTTGCGGCGTCAGTATGTCTTGTATCATT
AATCAAGGAAGCTGGAGTAAAGCCAATTTAAACATTTTAAGATGTGACAT
TTTCTATTTCATAACACAAAATCCCATTTTCATTGATTTTGCTGATTGAT
TTTACTCACGATAATTGATCAAACTGAGTTTTAGTTTTGTTTAAACAAAC
GTTTTTCATATTTTGTTTCATTGAAACATTGTGATATAGAAAATATTTTA
GACTTATGAATTTGATTTAATTAGT
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protein sequence of SMED30034859-orf-1

>SMED30034859-orf-1 ID=SMED30034859-orf-1|Name=SMED30034859-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=115bp
MTDNIVMYTIRRILALQQTEHIAIIDIIKNIAPIIIKAMATELNELSKYV
NICAPLTFRFEFEERLSKNDFKSGLSLSTTKSLAAVKSIPRTRRTIPIII
YIMLIRIKSVQQQQ*
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protein sequence of SMED30034859-orf-2

>SMED30034859-orf-2 ID=SMED30034859-orf-2|Name=SMED30034859-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=293bp
MGLFYCCCCTLLILINIIYMIIGIVLLVLGILLTAAKDFVVDKLSPLLKS
FFDSLSSNSNLNVNGAQMFTYLLNSLSSVAIALIIIGAIFFIISIIAMCS
VCCKARILLIVYITILSVIVLAQIIVIIIFAADPTKAKSIVEGILKDMII
KYEGFFSANPESFLINVMNIGLNCCGAINYQDFNASVKFNRTYSGTFTYG
GKTETYNVQLNYPISCCQFKEFQFVNSRECIENKGSNNKDKGCVTSLFDS
IQNYTTMTIQIICGIIGYQLLILLAASVCLVSLIKEAGVKPI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000099neuron
PLANA:0000418head
PLANA:0000420parapharyngeal region
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR018499Tetraspanin/Peripherin
IPR000301Tetraspanin
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018499Tetraspanin/PeripherinPFAMPF00335Tetraspanincoord: 10..280
e-value: 6.4E-24
score: 84.9
IPR000301TetraspaninPIRSFPIRSF002419Tetraspanincoord: 2..290
e-value: 2.4E-24
score: 83.5
NoneNo IPR availablePANTHERPTHR19282:SF216TETRASPANIN-1coord: 5..279
NoneNo IPR availablePANTHERPTHR19282TETRASPANINcoord: 5..279
NoneNo IPR availableTMHMMTMhelixcoord: 79..101
NoneNo IPR availableTMHMMTMhelixcoord: 262..284
NoneNo IPR availableTMHMMTMhelixcoord: 163..180
NoneNo IPR availableTMHMMTMhelixcoord: 108..130
NoneNo IPR availableTMHMMTMhelixcoord: 12..34