Membrane magnesium transporter 1

Overview
NameMembrane magnesium transporter 1
Smed IDSMED30034770
Length (bp)722
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Membrane magnesium transporter 1 (SMED30034770) t-SNE clustered cells

Violin plots show distribution of expression levels for Membrane magnesium transporter 1 (SMED30034770) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Membrane magnesium transporter 1 (SMED30034770) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Membrane magnesium transporter 1 (SMED30034770) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30034770

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30034770SMESG000074780.1 SMESG000074771.1 dd_Smed_v4_4513_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30034770SMESG000074780.1 SMESG000074771.1 dd_Smed_v4_4513_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30034770SMESG000074780.1 SMESG000074771.1 OX_Smed_1.0.17627ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Human
Match: MMGT1 (membrane magnesium transporter 1 [Source:HGNC Symbol;Acc:HGNC:28100])

HSP 1 Score: 89.3521 bits (220), Expect = 5.066e-22
Identity = 46/94 (48.94%), Postives = 68/94 (72.34%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFL 332
            K ++ IGL  L HAA+SA QHRSY+RLTE+   SLP D+++Q+++AF + C G V + G+FK++ AT++  +K +D + NHPSFY F HR + L
Sbjct:    7 KGLVGIGLFALAHAAFSAAQHRSYMRLTEKEDESLPIDIVLQTLLAFAVTCYGIVHIAGEFKDMDATSELKNKTFDTLRNHPSFYVFNHRGRVL 100
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Celegans
Match: emc-5 (EMC Endoplasmic Membrane protein Complex (Yeast EMC) homolog [Source:UniProtKB/TrEMBL;Acc:E1B6R8])

HSP 1 Score: 75.0998 bits (183), Expect = 6.790e-17
Identity = 42/94 (44.68%), Postives = 57/94 (60.64%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFL 332
            ++I I+ L  L H AYSA QHR YLRLTEQ F +LPSD++ Q+V++ I    GT  +   F  IK      D++ D   N PSF +F +RAK +
Sbjct:    7 RLITIVSLLSLLHCAYSAAQHRFYLRLTEQPFINLPSDVVAQTVISLIALIYGTSFLANPFLFIKKDQHH-DRSCDEALNCPSFITFDNRAKAM 99
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Fly
Match: CG15168 (gene:FBgn0032732 transcript:FBtr0081135)

HSP 1 Score: 94.3597 bits (233), Expect = 1.372e-24
Identity = 48/102 (47.06%), Postives = 68/102 (66.67%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSS 356
            K++LI G   L HAA+SA  HR+YLRLTEQ ++SLP D+++Q+V++ ++     + + G+FKEI+AT D   K WD +GN PSFYSF HR + L     P  
Sbjct:    8 KLLLIAGFASLAHAAFSAAHHRTYLRLTEQEWNSLPLDIILQTVISLVIVIYNIIEIVGNFKEIRATVDMQQKTWDTLGNFPSFYSFNHRGRALNPGYTPPE 109
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Zebrafish
Match: mmgt1 (membrane magnesium transporter 1 [Source:NCBI gene;Acc:402891])

HSP 1 Score: 87.8113 bits (216), Expect = 1.143e-21
Identity = 45/103 (43.69%), Postives = 72/103 (69.90%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQ 359
            K ++ IGL  L HAA+SA QHRSY+RLTE+   +LP D+++Q++++F++ C G V + G+FK++ A+++  +K +D + NHPSFY F HR + L  F  P  +
Sbjct:    7 KGVVGIGLFALAHAAFSAAQHRSYMRLTEKENETLPIDIVLQTLLSFVITCYGIVHISGEFKDMDASSELKNKTFDTLRNHPSFYLFNHRGRVL--FRTPEQE 107
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Zebrafish
Match: mmgt1 (membrane magnesium transporter 1 [Source:NCBI gene;Acc:402891])

HSP 1 Score: 87.8113 bits (216), Expect = 1.143e-21
Identity = 45/103 (43.69%), Postives = 72/103 (69.90%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQ 359
            K ++ IGL  L HAA+SA QHRSY+RLTE+   +LP D+++Q++++F++ C G V + G+FK++ A+++  +K +D + NHPSFY F HR + L  F  P  +
Sbjct:    7 KGVVGIGLFALAHAAFSAAQHRSYMRLTEKENETLPIDIVLQTLLSFVITCYGIVHISGEFKDMDASSELKNKTFDTLRNHPSFYLFNHRGRVL--FRTPEQE 107
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Zebrafish
Match: mmgt1 (membrane magnesium transporter 1 [Source:NCBI gene;Acc:402891])

HSP 1 Score: 74.7146 bits (182), Expect = 5.590e-17
Identity = 30/79 (37.97%), Postives = 54/79 (68.35%), Query Frame = 3 
Query:  123 LRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQ 359
            +RLTE+   +LP D+++Q++++F++ C G V + G+FK++ A+++  +K +D + NHPSFY F HR + L  F  P  +
Sbjct:    1 MRLTEKENETLPIDIVLQTLLSFVITCYGIVHISGEFKDMDASSELKNKTFDTLRNHPSFYLFNHRGRVL--FRTPEQE 77
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Xenopus
Match: mmgt1 (membrane magnesium transporter 1 [Source:NCBI gene;Acc:549307])

HSP 1 Score: 90.5077 bits (223), Expect = 1.759e-22
Identity = 48/103 (46.60%), Postives = 73/103 (70.87%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQ 359
            K ++ IGL  L HAA+SA QHRSY+RLTE+   +LP D+++Q+++AFI+ C G V + G+FK++ AT++  +K +D + NHPSFY F HR + +  F  P S+
Sbjct:    7 KGLVGIGLFALAHAAFSAAQHRSYMRLTEKEDETLPIDIVLQTLLAFIVACYGIVHIAGEFKDMDATSELRNKTFDTLRNHPSFYVFNHRGRVM--FQPPESE 107
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Xenopus
Match: mmgt1 (membrane magnesium transporter 1 [Source:NCBI gene;Acc:549307])

HSP 1 Score: 82.8037 bits (203), Expect = 1.993e-19
Identity = 36/82 (43.90%), Postives = 58/82 (70.73%), Query Frame = 3 
Query:  114 RSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQ 359
            RSY+RLTE+   +LP D+++Q+++AFI+ C G V + G+FK++ AT++  +K +D + NHPSFY F HR + +  F  P S+
Sbjct:   33 RSYMRLTEKEDETLPIDIVLQTLLAFIVACYGIVHIAGEFKDMDATSELRNKTFDTLRNHPSFYVFNHRGRVM--FQPPESE 112
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Mouse
Match: Mmgt2 (membrane magnesium transporter 2 [Source:MGI Symbol;Acc:MGI:2448491])

HSP 1 Score: 95.5153 bits (236), Expect = 1.210e-24
Identity = 45/102 (44.12%), Postives = 69/102 (67.65%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSS 356
            KV++ +GL  L HAA+SA QHRS+ RLTE+ +  LP+D+++Q+++AF + C G V   GDF++  AT++  D  +D + N PSFY F HR+ + + F  P S
Sbjct:    7 KVLMGVGLFALTHAAFSAAQHRSHARLTEKKYEPLPADIVLQTLLAFALTCYGVVHTAGDFRDRDATSELKDMTFDTLRNRPSFYVF-HRSGY-RLFQRPDS 106
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Mouse
Match: Mmgt2 (membrane magnesium transporter 2 [Source:MGI Symbol;Acc:MGI:2448491])

HSP 1 Score: 95.5153 bits (236), Expect = 1.210e-24
Identity = 45/102 (44.12%), Postives = 69/102 (67.65%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSS 356
            KV++ +GL  L HAA+SA QHRS+ RLTE+ +  LP+D+++Q+++AF + C G V   GDF++  AT++  D  +D + N PSFY F HR+ + + F  P S
Sbjct:    7 KVLMGVGLFALTHAAFSAAQHRSHARLTEKKYEPLPADIVLQTLLAFALTCYGVVHTAGDFRDRDATSELKDMTFDTLRNRPSFYVF-HRSGY-RLFQRPDS 106
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Mouse
Match: Mmgt1 (membrane magnesium transporter 1 [Source:MGI Symbol;Acc:MGI:2384305])

HSP 1 Score: 88.5817 bits (218), Expect = 6.781e-22
Identity = 45/94 (47.87%), Postives = 68/94 (72.34%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFL 332
            K ++ +GL  L HAA+SA QHRSY+RLTE+   SLP D+++Q+++AF + C G V + G+FK++ AT++  +K +D + NHPSFY F HR + L
Sbjct:    7 KGLVGVGLFALAHAAFSAAQHRSYMRLTEKEDESLPIDIVLQTLLAFAVTCYGIVHIAGEFKDMDATSELKNKTFDTLRNHPSFYVFNHRGRVL 100
[Close]
BLAST of Membrane magnesium transporter 1 vs. UniProt/SwissProt
Match: sp|Q8R3L0|MMGT2_MOUSE (Membrane magnesium transporter 2 OS=Mus musculus OX=10090 GN=Mmgt2 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 8.463e-24
Identity = 45/102 (44.12%), Postives = 69/102 (67.65%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSS 356
            KV++ +GL  L HAA+SA QHRS+ RLTE+ +  LP+D+++Q+++AF + C G V   GDF++  AT++  D  +D + N PSFY F HR+ + + F  P S
Sbjct:    7 KVLMGVGLFALTHAAFSAAQHRSHARLTEKKYEPLPADIVLQTLLAFALTCYGVVHTAGDFRDRDATSELKDMTFDTLRNRPSFYVF-HRSGY-RLFQRPDS 106
[Close]
BLAST of Membrane magnesium transporter 1 vs. UniProt/SwissProt
Match: sp|Q05AU4|MMG1B_XENLA (Membrane magnesium transporter 1-B OS=Xenopus laevis OX=8355 GN=mmgt1-b PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 8.469e-22
Identity = 48/103 (46.60%), Postives = 73/103 (70.87%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQ 359
            K ++ IGL  L HAA+SA QHRSY+RLTE+   +LP D+++Q+++AFI+ C G V + G+FK++ AT++  +K +D + NHPSFY F HR + +  F  P S+
Sbjct:    7 KGLVGIGLFALAHAAFSAAQHRSYMRLTEKEDETLPIDIVLQTLLAFIVACYGIVHIAGEFKDMDATSELRNKTFDTLRNHPSFYVFNHRGRVM--FQPPESE 107
[Close]
BLAST of Membrane magnesium transporter 1 vs. UniProt/SwissProt
Match: sp|Q28HV5|MMGT1_XENTR (Membrane magnesium transporter 1 OS=Xenopus tropicalis OX=8364 GN=mmgt1 PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 9.318e-22
Identity = 48/103 (46.60%), Postives = 73/103 (70.87%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQ 359
            K ++ IGL  L HAA+SA QHRSY+RLTE+   +LP D+++Q+++AFI+ C G V + G+FK++ AT++  +K +D + NHPSFY F HR + +  F  P S+
Sbjct:    7 KGLVGIGLFALAHAAFSAAQHRSYMRLTEKEDETLPIDIVLQTLLAFIVACYGIVHIAGEFKDMDATSELRNKTFDTLRNHPSFYVFNHRGRVM--FQPPESE 107
[Close]
BLAST of Membrane magnesium transporter 1 vs. UniProt/SwissProt
Match: sp|Q6GQ49|MMG1A_XENLA (Membrane magnesium transporter 1-A OS=Xenopus laevis OX=8355 GN=mmgt1-a PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.584e-21
Identity = 48/103 (46.60%), Postives = 72/103 (69.90%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQ 359
            K ++ IGL  L HAA+SA QHRSY+RLTE+   +LP D+++Q+++AFI+ C G V + G+FK + AT++  +K +D + NHPSFY F HR + +  F  P S+
Sbjct:    7 KGLVGIGLFALAHAAFSAAQHRSYMRLTEKEDETLPIDIVLQTLLAFIVACYGIVHIAGEFKHMDATSELRNKTFDTLRNHPSFYVFNHRGRIM--FQPPESE 107
[Close]
BLAST of Membrane magnesium transporter 1 vs. UniProt/SwissProt
Match: sp|Q8N4V1|MMGT1_HUMAN (Membrane magnesium transporter 1 OS=Homo sapiens OX=9606 GN=MMGT1 PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.433e-21
Identity = 46/94 (48.94%), Postives = 68/94 (72.34%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFL 332
            K ++ IGL  L HAA+SA QHRSY+RLTE+   SLP D+++Q+++AF + C G V + G+FK++ AT++  +K +D + NHPSFY F HR + L
Sbjct:    7 KGLVGIGLFALAHAAFSAAQHRSYMRLTEKEDESLPIDIVLQTLLAFAVTCYGIVHIAGEFKDMDATSELKNKTFDTLRNHPSFYVFNHRGRVL 100
[Close]
BLAST of Membrane magnesium transporter 1 vs. TrEMBL
Match: T1G6Y2 (Uncharacterized protein OS=Helobdella robusta OX=6412 GN=20216829 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 8.627e-30
Identity = 51/98 (52.04%), Postives = 69/98 (70.41%), Query Frame = 3 
Query:   39 MTNGKVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFL 332
            M+ GK+I+ +G+  LFHAAYSA QHRSYLR+TEQ F +LP D+LIQ++ A ++ C G   V G F+EI+A AD  ++ WD + +  SFY F HR K L
Sbjct:    1 MSFGKIIVTVGMITLFHAAYSAAQHRSYLRMTEQEFTNLPMDILIQTLFALVLTCCGVCKVVGSFREIRAVADLENRTWDSMNSRTSFYVFNHRGKKL 98
[Close]
BLAST of Membrane magnesium transporter 1 vs. TrEMBL
Match: R4G3S9 (Putative transmembrane protein 32 OS=Rhodnius prolixus OX=13249 PE=2 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.988e-29
Identity = 56/104 (53.85%), Postives = 66/104 (63.46%), Query Frame = 3 
Query:   39 MTNGKVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVP 350
            MT  K+I  +GL +L HAAYSA QHRSYLR+T Q F SLP D+LIQ +V+  +   G V V GDFKEI+AT     K W+   N PSFY F HR K L    VP
Sbjct:    1 MTTYKIITFLGLFLLLHAAYSAAQHRSYLRITGQEFTSLPLDILIQGIVSLFITMYGIVYVAGDFKEIRATVALESKTWETQRNLPSFYIFNHRGKMLSTTYVP 104
[Close]
BLAST of Membrane magnesium transporter 1 vs. TrEMBL
Match: A0A0A9WPY8 (Membrane magnesium transporter 1 OS=Lygus hesperus OX=30085 GN=mmgt1_0 PE=4 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 2.748e-29
Identity = 52/101 (51.49%), Postives = 66/101 (65.35%), Query Frame = 3 
Query:   42 TNGKVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFG 344
            T  K I  IGL  LFHAAYSA QHRS+LRLTEQ F SLP D+++Q +V+  +   G + + GDFKEI+AT +   K W+   N PSFY F HR K L+ + 
Sbjct:    4 TYHKTITAIGLIFLFHAAYSAAQHRSFLRLTEQEFTSLPVDVIVQGIVSLFLTMYGVMYIAGDFKEIRATEELQSKTWETQRNLPSFYCFNHRGKLLRPYS 104
[Close]
BLAST of Membrane magnesium transporter 1 vs. TrEMBL
Match: A0A183VIF1 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS1148 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 3.209e-29
Identity = 50/97 (51.55%), Postives = 72/97 (74.23%), Query Frame = 3 
Query:   36 VMTNGKVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAK 326
            ++   +  L++GL  L HAAYSA QHRSY+RLTEQ + SLP D+++Q V++F++ CIG V + G+ KEI+A A+F +K WD +GN PSFY+F HR +
Sbjct:    1 MVNTSRAALVVGLCGLIHAAYSAAQHRSYIRLTEQEYSSLPIDIVVQIVLSFVVVCIGLVGLTGNLKEIEAAAEFKEKTWDSLGNRPSFYTFHHRKQ 97
[Close]
BLAST of Membrane magnesium transporter 1 vs. TrEMBL
Match: A0A1I8M8U4 (Uncharacterized protein OS=Musca domestica OX=7370 GN=101898582 PE=4 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 6.346e-29
Identity = 50/101 (49.50%), Postives = 69/101 (68.32%), Query Frame = 3 
Query:   48 GKVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVP 350
             K++L+ G   L HAAYSA  +R+YLRLTEQ F  LP D+++Q+VV+ I+     + + G+FKEI+AT D   K+WD +GN PSFY+F HR K L +  VP
Sbjct:    7 NKILLVAGFASLLHAAYSAAHYRTYLRLTEQEFTKLPLDIVVQTVVSLIVVIYNVIQIVGNFKEIRATVDMQGKSWDTLGNFPSFYTFNHRGKALSSTYVP 107
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Cavefish
Match: mmgt1 (membrane magnesium transporter 1 [Source:NCBI gene;Acc:103029443])

HSP 1 Score: 85.8853 bits (211), Expect = 4.840e-21
Identity = 45/103 (43.69%), Postives = 71/103 (68.93%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQ 359
            K ++ +GL  L HAA+SA QHRSY+RLTE+   +LP D+++Q+++AF++ C G V + G+FK++ A+++   K +D + NHPSFY F HR + L  F  P  +
Sbjct:    7 KGVVGLGLFALAHAAFSAAQHRSYMRLTEKENETLPIDIVLQTLLAFVITCCGIVHISGEFKDMDASSELKHKTFDTLRNHPSFYLFNHRGRVL--FSSPDQE 107
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Sea Lamprey
Match: mmgt1 (membrane magnesium transporter 1 [Source:NCBI gene;Acc:402891])

HSP 1 Score: 78.9518 bits (193), Expect = 1.878e-19
Identity = 34/81 (41.98%), Postives = 53/81 (65.43%), Query Frame = 3 
Query:  114 RSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSS 356
            RSY RLTEQ   SLPSD+++Q+++  ++   G V + G+F+++ ATA+   K ++ + N PSFY+F HR + L  FG P  
Sbjct:    1 RSYTRLTEQENESLPSDIVLQTLLGLLVAVYGVVNIAGEFRDMDATAELKYKTFENMSNRPSFYTFNHRGRAL--FGQPEG 79
[Close]
BLAST of Membrane magnesium transporter 1 vs. Ensembl Nematostella
Match: EDO43490 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYN2])

HSP 1 Score: 109.383 bits (272), Expect = 1.150e-30
Identity = 48/103 (46.60%), Postives = 70/103 (67.96%), Query Frame = 3 
Query:   48 GKVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSS 356
            G+V++  G+  + HA YSAVQ R+Y +L E+ F SLP D+ IQ V+  ++ C+G V+V G+FKEIKA A+   K W+ +GN PSFY+F HR + + A  +P S
Sbjct:    5 GRVLVFFGVLAILHAGYSAVQCRTYYKLLEEEFVSLPLDIYIQCVLGMLLGCVGVVLVAGEFKEIKAAAEMASKTWESLGNRPSFYTFNHRGRAMFA-NLPQS 106
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BLAST of Membrane magnesium transporter 1 vs. Ensembl Medaka
Match: mmgt1 (membrane magnesium transporter 1 [Source:NCBI gene;Acc:101164072])

HSP 1 Score: 86.6557 bits (213), Expect = 2.804e-21
Identity = 44/103 (42.72%), Postives = 71/103 (68.93%), Query Frame = 3 
Query:   51 KVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQ 359
            K ++ +GL  L HAA+SA QHRSY+RLTE+   +LP D+ +Q++++F++ C G V + G+FK++ A+++  +K +D + NHPSFY F HR + L  F  P  +
Sbjct:    7 KGVVGVGLFALAHAAFSAAQHRSYMRLTEKENETLPIDIALQALLSFVITCYGIVHIAGEFKDMDASSELKNKTFDTLRNHPSFYLFNHRGRVL--FRTPEEE 107
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BLAST of Membrane magnesium transporter 1 vs. Planmine SMEST
Match: SMESG000074780.1 (SMESG000074780.1)

HSP 1 Score: 225.713 bits (574), Expect = 5.176e-76
Identity = 108/108 (100.00%), Postives = 108/108 (100.00%), Query Frame = 3 
Query:   39 MTNGKVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQL 362
            MTNGKVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQL
Sbjct:    1 MTNGKVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQL 108
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BLAST of Membrane magnesium transporter 1 vs. Planmine SMEST
Match: SMESG000074771.1 (SMESG000074771.1)

HSP 1 Score: 225.713 bits (574), Expect = 5.176e-76
Identity = 108/108 (100.00%), Postives = 108/108 (100.00%), Query Frame = 3 
Query:   39 MTNGKVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQL 362
            MTNGKVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQL
Sbjct:    1 MTNGKVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVAFIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKAFGVPSSQL 108
The following BLAST results are available for this feature:
BLAST of Membrane magnesium transporter 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MMGT15.066e-2248.94membrane magnesium transporter 1 [Source:HGNC Symb... [more]
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BLAST of Membrane magnesium transporter 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
emc-56.790e-1744.68EMC Endoplasmic Membrane protein Complex (Yeast EM... [more]
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BLAST of Membrane magnesium transporter 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG151681.372e-2447.06gene:FBgn0032732 transcript:FBtr0081135[more]
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BLAST of Membrane magnesium transporter 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
mmgt11.143e-2143.69membrane magnesium transporter 1 [Source:NCBI gene... [more]
mmgt11.143e-2143.69membrane magnesium transporter 1 [Source:NCBI gene... [more]
mmgt15.590e-1737.97membrane magnesium transporter 1 [Source:NCBI gene... [more]
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BLAST of Membrane magnesium transporter 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mmgt11.759e-2246.60membrane magnesium transporter 1 [Source:NCBI gene... [more]
mmgt11.993e-1943.90membrane magnesium transporter 1 [Source:NCBI gene... [more]
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BLAST of Membrane magnesium transporter 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Mmgt21.210e-2444.12membrane magnesium transporter 2 [Source:MGI Symbo... [more]
Mmgt21.210e-2444.12membrane magnesium transporter 2 [Source:MGI Symbo... [more]
Mmgt16.781e-2247.87membrane magnesium transporter 1 [Source:MGI Symbo... [more]
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BLAST of Membrane magnesium transporter 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8R3L0|MMGT2_MOUSE8.463e-2444.12Membrane magnesium transporter 2 OS=Mus musculus O... [more]
sp|Q05AU4|MMG1B_XENLA8.469e-2246.60Membrane magnesium transporter 1-B OS=Xenopus laev... [more]
sp|Q28HV5|MMGT1_XENTR9.318e-2246.60Membrane magnesium transporter 1 OS=Xenopus tropic... [more]
sp|Q6GQ49|MMG1A_XENLA1.584e-2146.60Membrane magnesium transporter 1-A OS=Xenopus laev... [more]
sp|Q8N4V1|MMGT1_HUMAN2.433e-2148.94Membrane magnesium transporter 1 OS=Homo sapiens O... [more]
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BLAST of Membrane magnesium transporter 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1G6Y28.627e-3052.04Uncharacterized protein OS=Helobdella robusta OX=6... [more]
R4G3S91.988e-2953.85Putative transmembrane protein 32 OS=Rhodnius prol... [more]
A0A0A9WPY82.748e-2951.49Membrane magnesium transporter 1 OS=Lygus hesperus... [more]
A0A183VIF13.209e-2951.55Uncharacterized protein OS=Trichobilharzia regenti... [more]
A0A1I8M8U46.346e-2949.50Uncharacterized protein OS=Musca domestica OX=7370... [more]
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BLAST of Membrane magnesium transporter 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mmgt14.840e-2143.69membrane magnesium transporter 1 [Source:NCBI gene... [more]
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BLAST of Membrane magnesium transporter 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mmgt11.878e-1941.98membrane magnesium transporter 1 [Source:NCBI gene... [more]
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BLAST of Membrane magnesium transporter 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Membrane magnesium transporter 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO434901.150e-3046.60Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Membrane magnesium transporter 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mmgt12.804e-2142.72membrane magnesium transporter 1 [Source:NCBI gene... [more]
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BLAST of Membrane magnesium transporter 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000074780.15.176e-76100.00SMESG000074780.1[more]
SMESG000074771.15.176e-76100.00SMESG000074771.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034770 ID=SMED30034770|Name=Membrane magnesium transporter 1|organism=Schmidtea mediterranea sexual|type=transcript|length=722bp
TTTTTTTTTCATTGGTTTAAAATTTTTTTTATATTGTGATGACGAATGGC
AAAGTCATTTTAATAATCGGGTTAACAGTTCTGTTTCATGCTGCATATTC
TGCTGTTCAACATCGATCATACTTACGCTTAACAGAACAAAACTTTCATA
GCTTACCAAGTGACCTATTAATTCAGAGCGTAGTAGCGTTTATTATGTGT
TGCATTGGAACAGTTATTGTTTGTGGGGATTTTAAAGAAATTAAAGCTAC
CGCAGATTTTGTTGATAAAAATTGGGATATAGTTGGAAATCATCCATCAT
TTTATTCATTCAGACACCGGGCTAAATTTTTGAAAGCATTTGGTGTTCCA
TCTAGTCAATTGTGATTTAATTTTTTACTTTTAGTTTATTTGTGTAATAA
ACTAATTATGTATTATATTTAGAATTTAAAAGGTTTATTATTAGTGTATT
TTTTTTAAAATGTAAAAATTTTGTCAAAATTATCAGTGACGTTTTTGCTT
TTGAACTGAGAAATCGTTTTTCTTTGTTGATAGTATTTTTTGGTACTCTA
ATAATTTCCTGTCGTTGATTTTAGTTTGAAGTTTTCCATTTGTTTATTAT
TTTCAAATAACTTTTATCTGCAAGGAAAGTTTTGGTATTAGCATGGAAGC
TAATATTTTAGTTCAATTTTGTATAGCGGAATTTTTACATTAAAGTTTAA
AATAAATCGTTTTGGTATTTAT
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protein sequence of SMED30034770-orf-1

>SMED30034770-orf-1 ID=SMED30034770-orf-1|Name=SMED30034770-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=109bp
MTNGKVILIIGLTVLFHAAYSAVQHRSYLRLTEQNFHSLPSDLLIQSVVA
FIMCCIGTVIVCGDFKEIKATADFVDKNWDIVGNHPSFYSFRHRAKFLKA
FGVPSSQL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0000139Golgi membrane
GO:0005769early endosome
GO:0005794Golgi apparatus
GO:0031901early endosome membrane
GO:0072546ER membrane protein complex
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR018937Magnesium_transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018937Magnesium transporterPFAMPF10270MMgTcoord: 9..77
e-value: 1.1E-14
score: 54.5
NoneNo IPR availablePANTHERPTHR21181FAMILY NOT NAMEDcoord: 3..99
NoneNo IPR availablePANTHERPTHR21181:SF7MEMBRANE MAGNESIUM TRANSPORTER 1coord: 3..99
NoneNo IPR availableTMHMMTMhelixcoord: 44..63
NoneNo IPR availableTMHMMTMhelixcoord: 7..29