SUMO-activating enzyme subunit 1

Overview
NameSUMO-activating enzyme subunit 1
Smed IDSMED30034728
Length (bp)1213
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SUMO-activating enzyme subunit 1 (SMED30034728) t-SNE clustered cells

Violin plots show distribution of expression levels for SUMO-activating enzyme subunit 1 (SMED30034728) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SUMO-activating enzyme subunit 1 (SMED30034728) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SUMO-activating enzyme subunit 1 (SMED30034728) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30034728

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30034728SMESG000036431.1 SmedASXL_009803SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30034728SMESG000036431.1 SmedASXL_009803SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
epidermisSMED30034728SMESG000036431.1 dd_Smed_v4_4443_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30034728SMESG000036431.1 dd_Smed_v4_4443_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30034728SMESG000036431.1 SMED_04010_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30034728SMESG000036431.1 SMED_04010_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Human
Match: SAE1 (SUMO1 activating enzyme subunit 1 [Source:HGNC Symbol;Acc:HGNC:30660])

HSP 1 Score: 175.637 bits (444), Expect = 7.605e-51
Identity = 108/358 (30.17%), Postives = 181/358 (50.56%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIV--VCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNP-----------SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSE------THNYATGTDIIA-----IFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSIKPE 1049
            I+EEE   YDRQIRLWGL+AQ RL+ ++VL++G+ GL  E+ K+L+L G+  + + D   V  ED    FLI   S+G NRA A+  R +NLNP V V+V         ++I  K      +FD V   CC+        ++ + +++ C K +  FF  D FG++G  F +L  HE+V E+ ++ K  +   + P           +  + + + FC   + L++DWS       LKR     F++  +  F ++      +  Y   ++++      + D+        G+  +LL   DF         PV AV+GG++ QEI+KA+S+++ P  NF  F+G+  + +   + P+
Sbjct:   11 ISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDT-------EDIEKKPESFFTQFDAVCLTCCSR-------DVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSL-------GISPDLLP-EDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK 346
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Human
Match: SAE1 (SUMO1 activating enzyme subunit 1 [Source:HGNC Symbol;Acc:HGNC:30660])

HSP 1 Score: 147.132 bits (370), Expect = 1.452e-40
Identity = 83/256 (32.42%), Postives = 133/256 (51.95%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIV--VCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNP-----------SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSE 776
            I+EEE   YDRQIRLWGL+AQ RL+ ++VL++G+ GL  E+ K+L+L G+  + + D   V  ED    FLI   S+G NRA A+  R +NLNP V V+V         ++I  K      +FD V   CC+        ++ + +++ C K +  FF  D FG++G  F +L  HE+V E+ ++ K  +   + P           +  + + + FC   + L++DWS       LKR     F++  ++  + E
Sbjct:   11 ISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDT-------EDIEKKPESFFTQFDAVCLTCCSR-------DVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQELFPRIVE 252
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Human
Match: SAE1 (SUMO1 activating enzyme subunit 1 [Source:HGNC Symbol;Acc:HGNC:30660])

HSP 1 Score: 144.05 bits (362), Expect = 1.111e-39
Identity = 82/247 (33.20%), Postives = 129/247 (52.23%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIV--VCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNP-----------SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIM 749
            I+EEE   YDRQIRLWGL+AQ RL+ ++VL++G+ GL  E+ K+L+L G+  + + D   V  ED    FLI   S+G NRA A+  R +NLNP V V+V         ++I  K      +FD V   CC+        ++ + +++ C K +  FF  D FG++G  F +L  HE+V E+ ++ K  +   + P           +  + + + FC   + L++DWS       LKR     F++
Sbjct:   11 ISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDT-------EDIEKKPESFFTQFDAVCLTCCSR-------DVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLL 243
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Human
Match: SAE1 (SUMO1 activating enzyme subunit 1 [Source:HGNC Symbol;Acc:HGNC:30660])

HSP 1 Score: 144.05 bits (362), Expect = 2.533e-39
Identity = 82/247 (33.20%), Postives = 129/247 (52.23%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIV--VCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNP-----------SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIM 749
            I+EEE   YDRQIRLWGL+AQ RL+ ++VL++G+ GL  E+ K+L+L G+  + + D   V  ED    FLI   S+G NRA A+  R +NLNP V V+V         ++I  K      +FD V   CC+        ++ + +++ C K +  FF  D FG++G  F +L  HE+V E+ ++ K  +   + P           +  + + + FC   + L++DWS       LKR     F++
Sbjct:   11 ISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDT-------EDIEKKPESFFTQFDAVCLTCCSR-------DVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLL 243
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Human
Match: SAE1 (SUMO1 activating enzyme subunit 1 [Source:HGNC Symbol;Acc:HGNC:30660])

HSP 1 Score: 134.806 bits (338), Expect = 1.533e-36
Identity = 72/196 (36.73%), Postives = 110/196 (56.12%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIV--VCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNP 629
            I+EEE   YDRQIRLWGL+AQ RL+ ++VL++G+ GL  E+ K+L+L G+  + + D   V  ED    FLI   S+G NRA A+  R +NLNP V V+V         ++I  K      +FD V   CC+        ++ + +++ C K +  FF  D FG++G  F +L  HE+V E+ ++ K  +   + P
Sbjct:   11 ISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDT-------EDIEKKPESFFTQFDAVCLTCCSR-------DVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGP 192
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Celegans
Match: aos-1 (SUMO-activating enzyme subunit aos-1 [Source:UniProtKB/Swiss-Prot;Acc:Q17820])

HSP 1 Score: 130.954 bits (328), Expect = 1.835e-34
Identity = 111/355 (31.27%), Postives = 167/355 (47.04%), Query Frame = 3 
Query:   51 KITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSR--VENLNPRV---IVE--VYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYV----------REEIEMQKPGEKPANNPSKWISEVLEFCSFDKVLQIDWSLASHARKLKRI-PKLQFIMLAMYNFVSETHNYATGT---DIIAIFDTW--QAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSIKP 1046
            ++++ E+ +YDRQIRLWG++AQN+++N+KVLIIG   L  EV K L L G++++ L D   V+ E+I  NFL + S         A S   + NLN  V   IVE  V +    EI + +  K T+    +VV   SY  + K      +N  CRK++  F     +GW G  F D   H Y+           EE E  K          +++ E   + SF + L  D++     RK KRI P   F++ +M    SE  N  TG    DI  +   W  +  A  H +D+  +    F+   G    P  A +GG++ QE IK+IS+   P  N  ++ G   +    +  P
Sbjct:    9 EVSKAEQAIYDRQIRLWGMEAQNKIRNSKVLIIGGKQLGAEVAKTLSLAGVDEMHLVDHRLVDTEEIGMNFLYDASVDNSKMTKWAASYNFLYNLNRNVKLFIVEEDVLSKNDSEIEEYL-TKFTL----VVVLDESYERTAK------VNNICRKHHIRFISGAIYGWIGYAFFDFDGHAYLVKAKSPDCLNEEESETGKTS-TVVTVDEEFVLETFSYPSFVETLNSDFTAKKIVRKCKRIVPTSYFLVKSMLRASSE--NKLTGVTENDIEKLIPIWNEEVAAGNHTIDMQPVQPDRFDHLFGPNFGPTAACVGGVIGQEAIKSISEGKNPLRNLFIYTGFESTGFMCNFPP 349
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Celegans
Match: ula-1 (NEDD8-activating enzyme E1 regulatory subunit [Source:UniProtKB/Swiss-Prot;Acc:Q18217])

HSP 1 Score: 80.1073 bits (196), Expect = 5.291e-16
Identity = 40/119 (33.61%), Postives = 60/119 (50.42%), Query Frame = 3 
Query:   78 YDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCC 434
            YDRQ+RLWG + Q  + +    ++G + L+ E++K LVL G+    + DD  V + DI  NF +    IG +RA A   ++  LNP V     +  P  +      KLT   F +VV  
Sbjct:   10 YDRQVRLWGEEGQASIGSTSACVLGSDSLATEILKSLVLAGVQSFYVVDDAKVEQADIGQNFFLHADDIGRSRAEATLEKLTELNPSVSGSASSQPPTALAMEDVEKLTT--FSVVVAA 126
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Celegans
Match: ula-1 (NEDD8-activating enzyme E1 regulatory subunit [Source:UniProtKB/Swiss-Prot;Acc:Q18217])

HSP 1 Score: 79.7221 bits (195), Expect = 5.822e-16
Identity = 40/119 (33.61%), Postives = 60/119 (50.42%), Query Frame = 3 
Query:   78 YDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCC 434
            YDRQ+RLWG + Q  + +    ++G + L+ E++K LVL G+    + DD  V + DI  NF +    IG +RA A   ++  LNP V     +  P  +      KLT   F +VV  
Sbjct:   10 YDRQVRLWGEEGQASIGSTSACVLGSDSLATEILKSLVLAGVQSFYVVDDAKVEQADIGQNFFLHADDIGRSRAEATLEKLTELNPSVSGSASSQPPTALAMEDVEKLTT--FSVVVAA 126
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Celegans
Match: uba-1 (UBA (Human ubiquitin) related [Source:UniProtKB/TrEMBL;Acc:Q27481])

HSP 1 Score: 56.6102 bits (135), Expect = 2.089e-8
Identity = 46/182 (25.27%), Postives = 80/182 (43.96%), Query Frame = 3 
Query:   66 EEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKD-NLWLNLNEKCRKYNKSFFGMDSFGWNGIMF--------VDLAHHEYVRE 584
            ++ +Y RQI   G  A   L+ A VLI G+  +  E+ K+L+L G+  + + D       D++  + +  + +G NRA +   R+  LN  V V+V         D +  +  ++ FD+VV   +   + +    W       R +N+     D+ G    +F        +D A  E VRE
Sbjct:  103 DKNLYSRQIYTLGESAMVNLRTASVLISGLGSVGVEIAKNLILGGVRHVTIHDTKLAKWSDLSAQYYLRDADVGHNRATSCYERLAELNDSVNVQVS-------TDELTEEF-VKTFDLVVLTDAARTAQRQIAAW------TRAHNRRILITDARGVFSYIFNDFGDNFRIDDATGEQVRE 270
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Celegans
Match: uba-1 (UBA (Human ubiquitin) related [Source:UniProtKB/TrEMBL;Acc:Q27481])

HSP 1 Score: 56.6102 bits (135), Expect = 2.164e-8
Identity = 46/182 (25.27%), Postives = 80/182 (43.96%), Query Frame = 3 
Query:   66 EEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKD-NLWLNLNEKCRKYNKSFFGMDSFGWNGIMF--------VDLAHHEYVRE 584
            ++ +Y RQI   G  A   L+ A VLI G+  +  E+ K+L+L G+  + + D       D++  + +  + +G NRA +   R+  LN  V V+V         D +  +  ++ FD+VV   +   + +    W       R +N+     D+ G    +F        +D A  E VRE
Sbjct:  104 DKNLYSRQIYTLGESAMVNLRTASVLISGLGSVGVEIAKNLILGGVRHVTIHDTKLAKWSDLSAQYYLRDADVGHNRATSCYERLAELNDSVNVQVS-------TDELTEEF-VKTFDLVVLTDAARTAQRQIAAW------TRAHNRRILITDARGVFSYIFNDFGDNFRIDDATGEQVRE 271
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Fly
Match: Aos1 (gene:FBgn0029512 transcript:FBtr0082593)

HSP 1 Score: 135.191 bits (339), Expect = 6.404e-36
Identity = 104/339 (30.68%), Postives = 165/339 (48.67%), Query Frame = 3 
Query:   51 KITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQ----KPGEKPANNPSKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSETHNYATGTDIIAIFDTWQA-FAVKHGLDINLLDISDF-EECIGV---GNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSI 1040
            ++TE E ++YDRQIRLWGL++Q RL+ AK+LI G+ GL  E+ K+++L+G+N + L DD +V EED  + FL+ R S+  NRA A+ +R   LNP V +        E            +FD+VV     +    +   L ++  CR     F   D +G  G  F  L  H YV + I+ +       +K     S      +++  +   L  D +  S+ RKLKR      ++  +  F + TH           + T +A   +  G+   LL  S   +E +G+      P  AV+GG+V QE+IK ++K  AP  N  +F+  +C+    +I
Sbjct:   13 ELTEAENELYDRQIRLWGLESQKRLRTAKILIAGLCGLGAEITKNIILSGVNSVKLLDDKDVTEEDFCSQFLVPRESLNTNRAEASLTRARALNPMVDISADREPLKEKTSEF-----FGQFDVVV-----VNGATNEELLRIDTICRDLGVKFIATDVWGTFGFYFASLQKHSYVEDVIKHKVVANSEKKKKYETVSIPTQRDVDYPGYSAWLDFDVTEPSYLRKLKRNGPGVLLLSVLQKFRT-THKRDPS------YKTREADLELLRGIRDELLPNSILGDEALGLIFAQISPAVAVVGGVVAQEVIKVVTKLEAPHRNLFVFDPETCAGYVEAI 334
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Fly
Match: APP-BP1 (gene:FBgn0261112 transcript:FBtr0076208)

HSP 1 Score: 80.4925 bits (197), Expect = 5.117e-16
Identity = 50/164 (30.49%), Postives = 78/164 (47.56%), Query Frame = 3 
Query:   78 YDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHH 569
            YDRQIRLWG   Q  L+ A V ++ +  + CE  K LVL GI    + D   V EED+ NNF ++ S +G+++A A    ++ LNP V  +  +     +  N  N    + FD+V+       +  +   L L E+  + N       S G  G + + +  H
Sbjct:   18 YDRQIRLWGEHGQTLLEAATVCLVNVTAVGCETAKGLVLPGIGGFTVADGSTVKEEDLGNNFFLDSSYLGKSKALACMQLLQELNPDVNGDYVDESADFLLANRPN--FFDSFDLVIAS-----NLNEQTLLLLAERLWELNVPLIYCRSLGMLGTIRLQIREH 174
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Fly
Match: APP-BP1 (gene:FBgn0261112 transcript:FBtr0331179)

HSP 1 Score: 80.4925 bits (197), Expect = 5.117e-16
Identity = 50/164 (30.49%), Postives = 78/164 (47.56%), Query Frame = 3 
Query:   78 YDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHH 569
            YDRQIRLWG   Q  L+ A V ++ +  + CE  K LVL GI    + D   V EED+ NNF ++ S +G+++A A    ++ LNP V  +  +     +  N  N    + FD+V+       +  +   L L E+  + N       S G  G + + +  H
Sbjct:   18 YDRQIRLWGEHGQTLLEAATVCLVNVTAVGCETAKGLVLPGIGGFTVADGSTVKEEDLGNNFFLDSSYLGKSKALACMQLLQELNPDVNGDYVDESADFLLANRPN--FFDSFDLVIAS-----NLNEQTLLLLAERLWELNVPLIYCRSLGMLGTIRLQIREH 174
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Fly
Match: Uba4 (gene:FBgn0032054 transcript:FBtr0079683)

HSP 1 Score: 65.0846 bits (157), Expect = 4.146e-11
Identity = 44/173 (25.43%), Postives = 81/173 (46.82%), Query Frame = 3 
Query:   42 IMNKITEEEEQVYDRQIRL--WGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFV 554
            +  K+T ++   Y RQ+ L  +G+  Q +LKN+ VLI+G+ GL C   + L   G   + L D   V   + +   L      G ++A +A+  +  LNP   ++ ++ M +      N    I  +D+V+ CT  +P+     +L L++ C   +K      +   +G + V
Sbjct:   60 VHTKLTNDDIARYSRQLILPDFGVQGQLKLKNSSVLIVGLGGLGCPAAQYLAAAGCGHLGLVDYDEVERSNFHRQILHSEDRCGMSKAESARIALLELNPHCEIQCHSRMLYP----HNAMHIIRGYDVVLDCTDNVPTR----YL-LSDACVMLSKPLVSGSALKMDGQLTV 223
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Fly
Match: Uba4 (gene:FBgn0032054 transcript:FBtr0333414)

HSP 1 Score: 65.0846 bits (157), Expect = 4.146e-11
Identity = 44/173 (25.43%), Postives = 81/173 (46.82%), Query Frame = 3 
Query:   42 IMNKITEEEEQVYDRQIRL--WGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFV 554
            +  K+T ++   Y RQ+ L  +G+  Q +LKN+ VLI+G+ GL C   + L   G   + L D   V   + +   L      G ++A +A+  +  LNP   ++ ++ M +      N    I  +D+V+ CT  +P+     +L L++ C   +K      +   +G + V
Sbjct:   60 VHTKLTNDDIARYSRQLILPDFGVQGQLKLKNSSVLIVGLGGLGCPAAQYLAAAGCGHLGLVDYDEVERSNFHRQILHSEDRCGMSKAESARIALLELNPHCEIQCHSRMLYP----HNAMHIIRGYDVVLDCTDNVPTR----YL-LSDACVMLSKPLVSGSALKMDGQLTV 223
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Zebrafish
Match: sae1 (SUMO1 activating enzyme subunit 1 [Source:NCBI gene;Acc:415148])

HSP 1 Score: 172.17 bits (435), Expect = 1.119e-49
Identity = 112/341 (32.84%), Postives = 171/341 (50.15%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAH-HEYVREEIEMQK---------PGEKPANNP--SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVS----ETHNYATGTDIIAIFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAV 1028
            I+EEE   YDRQIRLWGLDAQ RL+ ++VL++G+ GL  EV K+L+L G+  + L D   V EE     FLI   + G+N A A+  R + LNP V V+          D+        +FD  VC T        +L + +++ C   N   F  D +G+NG MF DL   + YV E+ ++ K           +KP  +P  +  + + + FCS  + L++DW+       LKRIP   F++  +  F +    +    +   D   +           GL  +LL  +    C    + PV AV+GG++ QEI+KA+S+++AP  NF  F+G+  S V
Sbjct:   12 ISEEEAAQYDRQIRLWGLDAQKRLRGSRVLLVGLRGLGAEVAKNLILAGVKGLTLLDHEQVTEESRRAQFLIPVDADGQNHAQASLERAQFLNPMVEVKADTEPVESKPDDF-----FFQFD-AVCLTRC----SRDLMVRVDQLCASRNIKVFCGDVYGYNGYMFSDLGQEYHYVEEKPKVVKGSNEANDGPEAKKPKIDPNETTMVKKTISFCSLKEALEVDWTTEKAKSSLKRIPADYFLLQVLLKFRTDKGRDPQPDSFAEDSQLLLQIRDDVLETMGLSSDLLPNTFVSYCFSEMS-PVCAVVGGVLGQEIVKALSQRDAPHRNFFFFDGLKGSGV 341
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Zebrafish
Match: nae1 (nedd8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:573336])

HSP 1 Score: 85.5001 bits (210), Expect = 1.248e-17
Identity = 45/114 (39.47%), Postives = 62/114 (54.39%), Query Frame = 3 
Query:   45 MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDN 386
            M      +EQ YDRQ+RLWG   Q  L+NA V +I       E++K+LVL GI    + D   V+ ED+ NNF +  S+IG+NRA AA   ++ LN  V        P ++ DN
Sbjct:    1 MTDPKTSKEQKYDRQLRLWGDHGQEALENAHVCLINATASGTEILKNLVLPGIGAFTIVDGHKVSGEDVGNNFFLSSSAIGKNRAQAATELLQELNSDVSGNFVEESPDKLLDN 114
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Zebrafish
Match: nae1 (nedd8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:573336])

HSP 1 Score: 85.5001 bits (210), Expect = 1.248e-17
Identity = 45/114 (39.47%), Postives = 62/114 (54.39%), Query Frame = 3 
Query:   45 MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDN 386
            M      +EQ YDRQ+RLWG   Q  L+NA V +I       E++K+LVL GI    + D   V+ ED+ NNF +  S+IG+NRA AA   ++ LN  V        P ++ DN
Sbjct:    1 MTDPKTSKEQKYDRQLRLWGDHGQEALENAHVCLINATASGTEILKNLVLPGIGAFTIVDGHKVSGEDVGNNFFLSSSAIGKNRAQAATELLQELNSDVSGNFVEESPDKLLDN 114
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Zebrafish
Match: uba7 (ubiquitin-like modifier activating enzyme 7 [Source:ZFIN;Acc:ZDB-GENE-121120-4])

HSP 1 Score: 73.9442 bits (180), Expect = 1.065e-13
Identity = 47/169 (27.81%), Postives = 83/169 (49.11%), Query Frame = 3 
Query:   66 EEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT---IEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLA 563
            +E +Y RQ+ + G DA  R+  A VLI G+ GL  E+ K+++L G+  + + D+  V   D+++ F ++ + +G+NRA  ++ ++ +LN  V V               NKL    + +F +VV  +S +     +  L +   C   N  F   D+ G  G +F D  
Sbjct:    7 DEGLYSRQLYVIGHDAMRRMGKADVLIAGMRGLGVEIAKNVILAGVRTVTIQDEGVVEWRDLSSQFYLKEADLGQNRALCSEKQLSSLNAYVKVSAS-----------TNKLDENFLSKFQVVVLTSSPL-----DEQLRVGAFCHSNNIKFIVADTRGLCGQLFCDFG 159

HSP 2 Score: 61.2326 bits (147), Expect = 1.351e-9
Identity = 37/125 (29.60%), Postives = 58/125 (46.40%), Query Frame = 3 
Query:   78 YDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGI-----NKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNP---------RVIV---EVYNHMPFEICDNINNKL 401
            YD QI ++G D QN+LK  K  ++G   + CE++K+  L G+       I + D  ++   ++N  FL     IG  ++ AA   V+ +NP         RV     EVY H  +   D +   L
Sbjct:  409 YDGQIAVFGSDFQNKLKKQKYFLVGAGAIGCELLKNFALIGLGAGEGGSITVTDMDSIERSNLNRQFLFRSQDIGRPKSEAAAEAVKEMNPFMNIIAQQNRVCAETEEVYTHSFYTGLDGVAAAL 533
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Zebrafish
Match: uba7 (ubiquitin-like modifier activating enzyme 7 [Source:ZFIN;Acc:ZDB-GENE-121120-4])

HSP 1 Score: 73.9442 bits (180), Expect = 1.065e-13
Identity = 47/169 (27.81%), Postives = 83/169 (49.11%), Query Frame = 3 
Query:   66 EEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT---IEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLA 563
            +E +Y RQ+ + G DA  R+  A VLI G+ GL  E+ K+++L G+  + + D+  V   D+++ F ++ + +G+NRA  ++ ++ +LN  V V               NKL    + +F +VV  +S +     +  L +   C   N  F   D+ G  G +F D  
Sbjct:    7 DEGLYSRQLYVIGHDAMRRMGKADVLIAGMRGLGVEIAKNVILAGVRTVTIQDEGVVEWRDLSSQFYLKEADLGQNRALCSEKQLSSLNAYVKVSAS-----------TNKLDENFLSKFQVVVLTSSPL-----DEQLRVGAFCHSNNIKFIVADTRGLCGQLFCDFG 159

HSP 2 Score: 61.2326 bits (147), Expect = 1.351e-9
Identity = 37/125 (29.60%), Postives = 58/125 (46.40%), Query Frame = 3 
Query:   78 YDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGI-----NKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNP---------RVIV---EVYNHMPFEICDNINNKL 401
            YD QI ++G D QN+LK  K  ++G   + CE++K+  L G+       I + D  ++   ++N  FL     IG  ++ AA   V+ +NP         RV     EVY H  +   D +   L
Sbjct:  409 YDGQIAVFGSDFQNKLKKQKYFLVGAGAIGCELLKNFALIGLGAGEGGSITVTDMDSIERSNLNRQFLFRSQDIGRPKSEAAAEAVKEMNPFMNIIAQQNRVCAETEEVYTHSFYTGLDGVAAAL 533
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Xenopus
Match: sae1 (SUMO1 activating enzyme subunit 1 [Source:NCBI gene;Acc:549624])

HSP 1 Score: 195.282 bits (495), Expect = 2.304e-58
Identity = 124/352 (35.23%), Postives = 186/352 (52.84%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQK-----------PGEKPAN-NPSKWI--SEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVS------ETHNYATGTDIIA-----IFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAV 1028
            I+EEE   YDRQIRLWGL+AQ RL+ ++VL++G+ GL  EV K+L+L G+  + L D   V+ ED    FLI   S+G+NRA A+ +R  NLNP V VE          +NIN K      +FD VVC TS       +L + ++  C K+N  FF  D FG++G MF DL  HE+V E+ ++ K           P  K A  +P++ I   + ++FC     L+IDW        LK+ P   F++  +  F +      +  NY   ++++      + D+        G+  +LL   DF         PV AV+GG++ QEI+KA+S+++AP  NF  F+G S + +
Sbjct:    9 ISEEEAAQYDRQIRLWGLEAQKRLRTSRVLLVGMRGLGAEVAKNLILAGVKALTLLDHEQVSSEDSRAQFLIPSGSLGQNRAEASLNRARNLNPMVSVEADT-------ENINQKSDDFFTQFD-VVCLTSC----SRDLLVRVDHICHKHNIKFFTGDVFGYHGYMFADLGEHEFVEEKAKVAKVSKAKQEVEDGPEAKKAKIDPTESILVKKKVQFCPLKDALEIDWHSEKAKSALKKTPTDFFLLQVLMKFRTDKKRDPQPSNYQEDSELLLQICSDVLDSL-------GVSPDLLP-KDFASYCFSEMAPVCAVVGGVLGQEIVKALSQRDAPHNNFFFFDGRSSNGI 340
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Xenopus
Match: gng2 (guanine nucleotide binding protein (G protein), gamma 2 [Source:Xenbase;Acc:XB-GENE-961282])

HSP 1 Score: 81.2629 bits (199), Expect = 4.573e-16
Identity = 53/171 (30.99%), Postives = 84/171 (49.12%), Query Frame = 3 
Query:   66 EEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYV 578
            +EQ YDRQ+RLWG   Q  L++A V +I       E++K+LVL GI    + D   V  ED+ NNF + R SIG+NRA  +   ++ LN  V        P ++ +   +     +F IV+  T+ +P   ++  L L E     N       ++G+ G M + +  H  +
Sbjct:    8 KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIVDGNVVTGEDVGNNFFLTRESIGKNRAQTSMELLQELNDDVTGNFVPESPEQLLEKDPSFFC--KFTIVI--TTQLP---ESTLLRLAETLWDSNIPLLVCRTYGFVGYMRIVVKEHTVI 171
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Xenopus
Match: igsf9b (immunoglobulin superfamily member 9B [Source:Xenbase;Acc:XB-GENE-5887265])

HSP 1 Score: 59.3066 bits (142), Expect = 5.137e-9
Identity = 36/121 (29.75%), Postives = 59/121 (48.76%), Query Frame = 3 
Query:  114 QNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLN 476
            Q  L+  K+L++G  GL CE++K+L L+G  +I + D   ++  ++N  FL     +G  +A  A   +    P   V  Y   PF +  +I N +    F I+VC    I + +   WLN
Sbjct:   30 QFLLETCKILVVGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDVGRPKAEVAAEFINTRIPDCCVTPY---PFTVNSSILNLMVSSLFHIIVCGLDSIIARR---WLN 144
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Xenopus
Match: gng2 (guanine nucleotide binding protein (G protein), gamma 2 [Source:Xenbase;Acc:XB-GENE-961282])

HSP 1 Score: 55.8398 bits (133), Expect = 7.190e-8
Identity = 47/178 (26.40%), Postives = 76/178 (42.70%), Query Frame = 3 
Query:   45 MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYV 578
            M +    +EQ YDRQ+RLWG   Q  L++A V +I       E++K+LVL GI    + D   V  ED              NRA  +   ++ LN  V        P ++ +   +     +F IV+  T+ +P   ++  L L E     N       ++G+ G M + +  H  +
Sbjct:    1 MAQAVHIKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIVDGNVVTGED--------------NRAQTSMELLQELNDDVTGNFVPESPEQLLEKDPSFFC--KFTIVI--TTQLP---ESTLLRLAETLWDSNIPLLVCRTYGFVGYMRIVVKEHTVI 157
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Xenopus
Match: gng2 (guanine nucleotide binding protein (G protein), gamma 2 [Source:Xenbase;Acc:XB-GENE-961282])

HSP 1 Score: 55.8398 bits (133), Expect = 7.347e-8
Identity = 28/71 (39.44%), Postives = 39/71 (54.93%), Query Frame = 3 
Query:   45 MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINN 257
            M +    +EQ YDRQ+RLWG   Q  L++A V +I       E++K+LVL GI    + D   V  ED+ N
Sbjct:    1 MAQAVHIKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIVDGNVVTGEDVGN 71
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Mouse
Match: Sae1 (SUMO1 activating enzyme subunit 1 [Source:MGI Symbol;Acc:MGI:1929264])

HSP 1 Score: 174.481 bits (441), Expect = 1.590e-50
Identity = 105/350 (30.00%), Postives = 179/350 (51.14%), Query Frame = 3 
Query:   78 YDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIV--VCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNP-----------SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSE------THNYATGTDIIA-----IFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSIKPE 1049
            YDRQIRLWGL+AQ RL+ ++VLI+G+ GL  E+ K+L+L G+  + + D   V+ ED    FLI+  S+G NRA A+  R +NLNP V V+V         +++  K      +FD V   CC+        ++ + +++ C + +  FF  D FG++G  F +L  HE+V E+ ++ K  +   + P           +  + + + FC   + L++DWS       LKR     F++  +  F ++      + +Y    +++      +FD+        G+  +LL   DF         PV AV+GG++ QEI+KA+S+++ P  NF  F+G+  S +   + P+
Sbjct:   23 YDRQIRLWGLEAQKRLRASRVLIVGMKGLGAEIAKNLILAGVKGLTMLDHEQVSPEDPGAQFLIQTGSVGRNRAEASLERAQNLNPMVDVKVDT-------EDVEKKPESFFTKFDAVCLTCCSR-------DVIIKVDQICHRNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPEAKRAKLDSSETTMVKKKVLFCPVKEALEVDWSGEKAKAALKRTAPDYFLLQVLLKFRTDKGRDPTSESYKEDAELLLQIRNDVFDSL-------GISPDLLP-DDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGSGIVECLGPQ 350
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Mouse
Match: Sae1 (SUMO1 activating enzyme subunit 1 [Source:MGI Symbol;Acc:MGI:1929264])

HSP 1 Score: 174.481 bits (441), Expect = 1.590e-50
Identity = 105/350 (30.00%), Postives = 179/350 (51.14%), Query Frame = 3 
Query:   78 YDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIV--VCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNP-----------SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSE------THNYATGTDIIA-----IFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSIKPE 1049
            YDRQIRLWGL+AQ RL+ ++VLI+G+ GL  E+ K+L+L G+  + + D   V+ ED    FLI+  S+G NRA A+  R +NLNP V V+V         +++  K      +FD V   CC+        ++ + +++ C + +  FF  D FG++G  F +L  HE+V E+ ++ K  +   + P           +  + + + FC   + L++DWS       LKR     F++  +  F ++      + +Y    +++      +FD+        G+  +LL   DF         PV AV+GG++ QEI+KA+S+++ P  NF  F+G+  S +   + P+
Sbjct:   23 YDRQIRLWGLEAQKRLRASRVLIVGMKGLGAEIAKNLILAGVKGLTMLDHEQVSPEDPGAQFLIQTGSVGRNRAEASLERAQNLNPMVDVKVDT-------EDVEKKPESFFTKFDAVCLTCCSR-------DVIIKVDQICHRNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPEAKRAKLDSSETTMVKKKVLFCPVKEALEVDWSGEKAKAALKRTAPDYFLLQVLLKFRTDKGRDPTSESYKEDAELLLQIRNDVFDSL-------GISPDLLP-DDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGSGIVECLGPQ 350
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Mouse
Match: Sae1 (SUMO1 activating enzyme subunit 1 [Source:MGI Symbol;Acc:MGI:1929264])

HSP 1 Score: 151.754 bits (382), Expect = 3.514e-42
Identity = 96/320 (30.00%), Postives = 161/320 (50.31%), Query Frame = 3 
Query:   78 YDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIV--VCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNP-----------SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSE------THNYATGTDIIA-----IFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIK 959
            YDRQIRLWGL+AQ RL+ ++VLI+G+ GL  E+ K+L+L G+  + + D   V+ ED    FLI+  S+G NRA A+  R +NLNP V V+V         +++  K      +FD V   CC+        ++ + +++ C + +  FF  D FG++G  F +L  HE+V E+ ++ K  +   + P           +  + + + FC   + L++DWS       LKR     F++  +  F ++      + +Y    +++      +FD+        G+  +LL   DF         PV AV+GG++ QEI+K
Sbjct:   23 YDRQIRLWGLEAQKRLRASRVLIVGMKGLGAEIAKNLILAGVKGLTMLDHEQVSPEDPGAQFLIQTGSVGRNRAEASLERAQNLNPMVDVKVDT-------EDVEKKPESFFTKFDAVCLTCCSR-------DVIIKVDQICHRNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPEAKRAKLDSSETTMVKKKVLFCPVKEALEVDWSGEKAKAALKRTAPDYFLLQVLLKFRTDKGRDPTSESYKEDAELLLQIRNDVFDSL-------GISPDLLP-DDFVRYCFSEMAPVCAVVGGILAQEIVK 320
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Mouse
Match: Uba7 (ubiquitin-like modifier activating enzyme 7 [Source:MGI Symbol;Acc:MGI:1349462])

HSP 1 Score: 83.5741 bits (205), Expect = 4.727e-17
Identity = 53/178 (29.78%), Postives = 90/178 (50.56%), Query Frame = 3 
Query:   66 EEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQ 599
            +E++Y RQ+ + GL A  R++ AKVL+ G+ GL  EV K+LVLTG+  + L D       D+     +   S+G NRA A+++++  LN  V + V++        +I   L ++ F +VV   S +   +D   L +   C K+   F   ++ G  G +F D      V +  E++
Sbjct:    2 DEELYSRQLYVLGLPAMQRIQEAKVLLCGLQGLGAEVAKNLVLTGVGSLTLHDPHPTCWADLAAQCFLSEESLGRNRAEASQAQLAQLNEAVQISVHS-------GDITEDL-LQGFQVVVLTDSKL---EDQ--LKVGPLCHKHGVRFLMAETRGLVGRLFCDFGEDFTVLDPTEVE 166
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Mouse
Match: Nae1 (NEDD8 activating enzyme E1 subunit 1 [Source:MGI Symbol;Acc:MGI:2384561])

HSP 1 Score: 83.1889 bits (204), Expect = 7.859e-17
Identity = 52/138 (37.68%), Postives = 71/138 (51.45%), Query Frame = 3 
Query:   66 EEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCT---SYIPSFKDNLW 470
            +EQ YDRQ+RLWG   Q  L++A V +I       E++K+LVL GI    + D   V+ ED  NNF +++SSIG+NRA AA   ++ LN  V        P  + DN  +      F IVV      S +    D LW
Sbjct:    9 KEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNLVSGEDAGNNFFLQKSSIGKNRAQAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFC--RFTIVVATQLLESTLLRLADVLW 144
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. UniProt/SwissProt
Match: sp|Q28DS0|SAE1_XENTR (SUMO-activating enzyme subunit 1 OS=Xenopus tropicalis OX=8364 GN=sae1 PE=2 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 1.221e-57
Identity = 124/352 (35.23%), Postives = 186/352 (52.84%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQK-----------PGEKPAN-NPSKWI--SEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVS------ETHNYATGTDIIA-----IFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAV 1028
            I+EEE   YDRQIRLWGL+AQ RL+ ++VL++G+ GL  EV K+L+L G+  + L D   V+ ED    FLI   S+G+NRA A+ +R  NLNP V VE          +NIN K      +FD VVC TS       +L + ++  C K+N  FF  D FG++G MF DL  HE+V E+ ++ K           P  K A  +P++ I   + ++FC     L+IDW        LK+ P   F++  +  F +      +  NY   ++++      + D+        G+  +LL   DF         PV AV+GG++ QEI+KA+S+++AP  NF  F+G S + +
Sbjct:    9 ISEEEAAQYDRQIRLWGLEAQKRLRTSRVLLVGMRGLGAEVAKNLILAGVKALTLLDHEQVSSEDSRAQFLIPSGSLGQNRAEASLNRARNLNPMVSVEADT-------ENINQKSDDFFTQFD-VVCLTSC----SRDLLVRVDHICHKHNIKFFTGDVFGYHGYMFADLGEHEFVEEKAKVAKVSKAKQEVEDGPEAKKAKIDPTESILVKKKVQFCPLKDALEIDWHSEKAKSALKKTPTDFFLLQVLMKFRTDKKRDPQPSNYQEDSELLLQICSDVLDSL-------GVSPDLLP-KDFASYCFSEMAPVCAVVGGVLGQEIVKALSQRDAPHNNFFFFDGRSSNGI 340
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. UniProt/SwissProt
Match: sp|Q8JGT5|SAE1_XENLA (SUMO-activating enzyme subunit 1 OS=Xenopus laevis OX=8355 GN=sae1 PE=2 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 1.485e-57
Identity = 121/349 (34.67%), Postives = 186/349 (53.30%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQK--------PGEKPAN-NPSKWI--SEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVS------ETHNYATGTDIIA-----IFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAV 1028
            I+EEE   YDRQIRLWGL+AQ RL+ ++VL++G+ GL  EV K+L+L G+  + L D   V+ ED    FLI   S+G+NRA A+ +R  NLNP V VE          +NIN K      +FD+V  C +  PS   +L + +N  C K+N  FF  D +G++G MF DL  HE+V E+ ++ K        P  K A  +P++ I   + ++FC     L+IDW        LK+ P   F++  +  F +      +  +Y   ++++      + D+        G+  +LL   DF         PV AV+GG++ QEI+KA+S ++AP  NF  F+G + + +
Sbjct:    9 ISEEEAAQYDRQIRLWGLEAQKRLRTSRVLLVGMRGLGAEVAKNLILAGVKALTLLDHEQVSSEDSRAQFLIPSGSLGQNRAEASLNRARNLNPMVSVEADT-------ENINQKSDDFFTQFDVV--CLTSCPS---DLLVRVNHICHKHNIKFFTGDVYGYHGSMFADLGEHEFVEEKAKVTKAKPLVEDGPEAKKAKIDPTETILVKKKVQFCPLKDALEIDWRSEKAKSALKKTPTDYFLLQVLMKFRTDKGRDPQPSSYQEDSELLLQICSDVLDSL-------GVSPDLLP-KDFASYCFSEMAPVCAVVGGVLGQEIVKALSLRDAPHNNFFFFDGKTSNGI 337
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. UniProt/SwissProt
Match: sp|A2VE14|SAE1_BOVIN (SUMO-activating enzyme subunit 1 OS=Bos taurus OX=9913 GN=SAE1 PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.308e-51
Identity = 110/350 (31.43%), Postives = 178/350 (50.86%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIV--VCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNP-----------SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSETHNYAT----GTDIIAIFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSIKP 1046
            I+EEE   YDRQIRLWGL+AQ RL+ ++VL++G+ GL  E+ K+L+L G+  + + D   V+ ED    FLI   S+G NRA A+  R +NLNP V V+V         +NI  K      +FD V   CC+        ++ + +++ C K +  FF  D FG++G  F +L  HE+V E+ ++ K  +   + P           +  + + + FCS  + L++DWS       LKR     F++  +  F ++     +    G D   +           G++ +LL   DF         PV AV+GG++ QEI+KA+S+++ P  NF  F+G+  + +   + P
Sbjct:   11 ISEEEAAQYDRQIRLWGLEAQKRLRASQVLLVGMKGLGAEIAKNLILAGVKGLTMLDHEQVSPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDT-------ENIEKKPESFFTQFDAVCLTCCSR-------DVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCSVKEALEVDWSSDKAKAALKRTTPDYFLLQVLLKFRTDKGRDPSSDTFGEDSELLLQIRNDVLDALGVNPDLLP-EDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGP 345
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. UniProt/SwissProt
Match: sp|Q6AXQ0|SAE1_RAT (SUMO-activating enzyme subunit 1 OS=Rattus norvegicus OX=10116 GN=Sae1 PE=2 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 7.827e-51
Identity = 107/350 (30.57%), Postives = 180/350 (51.43%), Query Frame = 3 
Query:   78 YDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIV--VCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNP-----------SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSE------THNYATGTDIIA-----IFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSIKPE 1049
            YDRQIRLWGL+AQ RL+ ++VLI+G+ GL  E+ K+L+L G+  + + D   V+ ED+   FLI   S+G+NRA A+  R +NLNP V V+V         ++I  K      EFD V   CC+        ++ + +++ C + +  FF  D FG++G  F +L  HE+V E+ ++ K  +   + P           +  + + + FC   + L +DWS       LKR     F++  +  F ++      + +Y+   +++      +FD+        G+  +LL   DF         PV AV+GG++ QEI+KA+S+++ P  NF  F+G+  S +   + P+
Sbjct:   22 YDRQIRLWGLEAQKRLRASRVLIVGMKGLGAEIAKNLILAGVKGLTMLDHEQVSPEDLGAQFLIRTGSVGQNRAEASLERAQNLNPMVDVKVDT-------EDIEKKPESFFTEFDAVCLTCCSK-------DVIIKVDQICHRNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVTKVSQGVEDGPDAKRAKLDSSETTMVKKKVLFCPVKEALAVDWSGEKAQAALKRTAPDYFLLQVLLKFRTDKGRDPTSDSYSEDAELLLQIRNDVFDSL-------GVSPDLLP-DDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGSGIVECLGPQ 349
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. UniProt/SwissProt
Match: sp|Q5NVN7|SAE1_PONAB (SUMO-activating enzyme subunit 1 OS=Pongo abelii OX=9601 GN=SAE1 PE=2 SV=2)

HSP 1 Score: 175.637 bits (444), Expect = 3.652e-50
Identity = 108/358 (30.17%), Postives = 181/358 (50.56%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIV--VCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNP-----------SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSE------THNYATGTDIIA-----IFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSIKPE 1049
            I+EEE   YDRQIRLWGL+AQ RL+ ++VL++G+ GL  E+ K+L+L G+  + + D   V  ED    FLI   S+G NRA A+  R +NLNP V V+V         ++I  K      +FD V   CC+        ++ + +++ C K +  FF  D FG++G  F +L  HE+V E+ ++ K  +   + P           +  + + + FC   + L++DWS       LKR     F++  +  F ++      +  Y   ++++      + D+        G+  +LL   DF         PV AV+GG++ QEI+KA+S+++ P  NF  F+G+  + +   + P+
Sbjct:   11 ISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDT-------EDIEKKPESFFTQFDAVCLTCCSR-------DVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSL-------GISPDLLP-EDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK 346
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. TrEMBL
Match: A0A0L8H8Z8 (ThiF domain-containing protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22019850mg PE=4 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 4.332e-59
Identity = 123/356 (34.55%), Postives = 194/356 (54.49%), Query Frame = 3 
Query:   42 IMNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNK--LTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGE-------KPANNPSKWISEVL------EFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSETHNYAT----GTDIIAIF----DTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSI 1040
            +  +++E+E  +YDRQIRLWGL+AQ RL+ AKVL++G++GL  EV K++VL+GIN + L D   V EED  N FL+ R  +G NRA A++   + LNP V + V          N+++K     ++F+ V+C TS       N    +N+ C ++N  FF  D+FG+ G MF DL  HE+  EEI   KP E       +P    +K   E +      EF  F+  L+ DWS   + ++LKR  K+ FIM  M  F+     + +     +D+  +     +  ++ +VK  +  +      F E       PV+A++GG+++QEIIKA+S+K+ P  NF  F+G++ + +   I
Sbjct:    2 VEERLSEDEAALYDRQIRLWGLEAQKRLRAAKVLLVGLSGLGAEVAKNIVLSGINSLTLLDHKKVTEEDRCNQFLVSRDDVGRNRAEASQEATQKLNPLVKITVDT-------SNVDDKPDTFFQQFN-VICITSSC----RNALCRINKLCHEHNIKFFATDAFGYFGFMFSDLGSHEFA-EEIPKPKPKETEESENGEPKEKRAKIAMETVVVKNSSEFVRFESALKFDWSTEENQKRLKRTAKVYFIMQVMLKFLERHGRHPSVKSKTSDMEELMKLRTEVLESLSVKQEVLTDYFVSHCFAEL-----CPVSAIVGGVLSQEIIKAVSQKDKPHNNFFFFDGINGTGLVEQI 339
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. TrEMBL
Match: A0A1S3IQB3 (SUMO-activating enzyme subunit 1 isoform X2 OS=Lingula unguis OX=7574 GN=LOC106166394 PE=4 SV=1)

HSP 1 Score: 199.904 bits (507), Expect = 4.794e-57
Identity = 125/336 (37.20%), Postives = 180/336 (53.57%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNPSKWISE-------VLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSETHN----YATGTDIIAIFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAV 1028
            +TE+E  +YDRQIRLWGLDAQ RL+ A+VL+IG+ GL  E+ K++VL GI  + L D   V EED  + FLI RS IGENRA A+  R   LNP V V   +    E      ++   + FD VVC T+ I   KD L L +N  C +    FF  D FG++G MF DL  HEY  E I+ +K   + +   +K   E        + F    + L +DWS  + ++ LK++PK  FI+  +  F ++        +   DI+ +         +  +  + +D S    C    + PV A++GG+  QEIIKA+S+K+AP  NF  FNG+  S +
Sbjct:    9 LTEDEAALYDRQIRLWGLDAQKRLRAARVLLIGLQGLGAEIAKNIVLAGIKSLTLMDHTVVTEEDACSQFLIPRSKIGENRAEASVERTALLNPMVEVTADSSDIAE-----KDETFFQNFD-VVCATNLI---KDQL-LRINSICHENKIKFFAGDVFGYHGFMFADLNEHEYAEETIKKKKESGEASAKKAKMQEEETVVSKKSMSFVRLKEALAVDWSSENMSKLLKKVPKTFFIIQVLLRFRAQYKRDPSPKSKEEDIVNLLKLRDQVLEELKISKDFVDDSFASYCFAELS-PVCAIVGGVFGQEIIKAVSQKDAPHNNFFFFNGLDGSGM 333
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. TrEMBL
Match: A0A1S3IQQ4 (SUMO-activating enzyme subunit 1 isoform X1 OS=Lingula unguis OX=7574 GN=LOC106166394 PE=4 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 6.463e-57
Identity = 125/336 (37.20%), Postives = 179/336 (53.27%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNPSKWISE-------VLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSETHN----YATGTDIIAIFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAV 1028
            +TE+E  +YDRQIRLWGLDAQ RL+ A+VL+IG+ GL  E+ K++VL GI  + L D   V EED  + FLI RS IGENRA A+  R   LNP V V   +    E      ++   + FD VVC T+ I   KD L L +N  C +    FF  D FG++G MF DL  HEY  E I+ +K     +   +K   E        + F    + L +DWS  + ++ LK++PK  FI+  +  F ++        +   DI+ +         +  +  + +D S    C    + PV A++GG+  QEIIKA+S+K+AP  NF  FNG+  S +
Sbjct:    9 LTEDEAALYDRQIRLWGLDAQKRLRAARVLLIGLQGLGAEISKNIVLAGIKSLTLMDHTVVTEEDACSQFLIPRSKIGENRAEASVERTALLNPMVEVTADSSDIAE-----KDETFFQNFD-VVCATNLI---KDQL-LRINSICHENKIKFFAGDVFGYHGFMFADLNEHEYAEETIKKKKESGDASAKKAKMQEEETVVSKKSMSFVRLKEALAVDWSSENMSKLLKKVPKTFFIIQVLLKFRAQHKRDPSPKSKEEDIVNLLKVRDQVLEELKISKDFVDDSFASYCFAELS-PVCAIVGGVFGQEIIKAVSQKDAPHNNFFFFNGLDGSGM 333
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. TrEMBL
Match: A0A401RNN6 (ThiF domain-containing protein OS=Chiloscyllium punctatum OX=137246 GN=chiPu_0018517 PE=4 SV=1)

HSP 1 Score: 201.06 bits (510), Expect = 8.705e-57
Identity = 119/353 (33.71%), Postives = 186/353 (52.69%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNP-----------SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSET------HNYATGTDIIAIFDT--WQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSIKPE 1049
            I+EEE   YDRQIRLWGLDAQ RL+ ++VL++G+ GL  EV K+L+L G+  + L D      ED  + FLI  SS+G+NRA A+  R +NLNP V V+V         +NI+NK    + +FD +  C +  P    ++ + +++ CR +   FF  D FG++G MF DL  H +V E+ ++ K G    + P           +  I + + FC   + L++DWS       LKR P   F++  +  F S         NY   ++++    +    +  VK  L  +    S F E       PV AV+GG+++QEI+KA+S+++ P  NF  F+G+  + +   I P+
Sbjct:   58 ISEEEAAQYDRQIRLWGLDAQKRLRGSRVLLVGMKGLGAEVAKNLILAGVKALTLLDHQQATPEDSRSQFLIPTSSLGKNRAEASLERAQNLNPMVEVKVDT-------ENISNKSQEFLAQFDAI--CLTCCPR---DVMVRIDQFCRSHGIKFFAGDVFGYHGYMFSDLGEHTFVEEKQKVVKVGSGEEDGPKAKKVKIDPNETTLIKKTVHFCHLKEALEVDWSSEKAKTCLKRTPSDYFLLQVLLKFRSAKARDPLPENYDEDSELLLQLRSKVLDSLGVKSDLLTDEFVSSCFSEL-----APVCAVVGGVLSQEIVKALSQRDLPHNNFFFFDGLKSNGIVDCIGPK 393
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. TrEMBL
Match: A0A2C9JTP8 (ThiF domain-containing protein OS=Biomphalaria glabrata OX=6526 GN=106067859 PE=4 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 2.596e-56
Identity = 130/337 (38.58%), Postives = 188/337 (55.79%), Query Frame = 3 
Query:   45 MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKP--GEKPANNPSKWIS--EVLEFCSFDKVLQIDWSLASHA--RKLKRIPKLQFIMLAMYNFVSETHNYATGTDIIAIFDTWQAFAVKHGLDINLLDIS------DFEECIGVGNV-PVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVS 1016
            M+ ITE+E ++YDRQIRLWGLDAQ RL+ A VL+IG+ GL  EV K+++L+GI  I L D   V+EED  + F +   + G+NRA A+ S  + LNP V V    + P E  DN       +EFD V+C T   PS    L   L++ C + N  FF  D +G+ G  F DL  HEY+ EE+++ KP  GE  +    K I+  E + F   +  LQ DW+  + A  +K+++ P   FI      F+ E H        +   DT +   ++  L +N L +       DF +  G G + PV A++GG++ QEIIKA+S+K+AP  NF  +NGV+
Sbjct:    7 MDLITEDEAELYDRQIRLWGLDAQRRLRAASVLLIGLKGLGAEVAKNIILSGIKAITLMDHNPVDEEDQMSQFFVSTVAPGQNRAEASASNAQLLNPMVTVTTDTNNPAEKDDNF-----FKEFD-VICATCCPPS----LLYRLDKLCFENNIKFFAGDVYGYYGFTFSDLGTHEYLHEEVKVLKPKEGEGQSKTTEKTITSKEKIVFTRLEDELQFDWTADTPAMQKKIRQTPSTFFITRVYQEFL-EVHGRRPCISSLEA-DTKELQNIRLQL-LNKLGVPAKVIPEDFYK-YGFGELSPVCAIVGGILGQEIIKAVSQKDAPLNNFFFYNGVT 329
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Cavefish
Match: sae1 (SUMO1 activating enzyme subunit 1 [Source:NCBI gene;Acc:415148])

HSP 1 Score: 97.4413 bits (241), Expect = 3.956e-23
Identity = 64/209 (30.62%), Postives = 106/209 (50.72%), Query Frame = 3 
Query:  462 NLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAH-HEYVREEIEMQKPGE---------KPANNP--SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSE------THNYATGTDIIAIF--DTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAV 1028
            +L +++++ C   N   F  D FG++G MF DL   H YV E+ ++ KP E         KP  +P  +  + +   FCS  + L+IDW+      +LKR P   F++  +  F ++        +YA  + ++     D  +A     G++ +LL  S    C      PV AV+GG++ QEI+KA+S+++AP  NF  F+G+  S V
Sbjct:   10 DLMMHVDQLCASKNIKVFCGDVFGYHGYMFSDLGQEHHYVEEKPKIVKPSEETNDGPEVKKPKIDPNETTMVKKTASFCSLKEALEIDWTSEEVKGRLKRTPSDYFLLQVLLKFRTDKGRNPDPASYAEDSQLLLQIRDDVLEAL----GVNADLLPDSFVSYCFS-EMAPVCAVVGGVLGQEIVKALSQRDAPHRNFFFFDGLKGSGV 213
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Cavefish
Match: nae1 (NEDD8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:103042317])

HSP 1 Score: 82.0333 bits (201), Expect = 1.595e-16
Identity = 42/114 (36.84%), Postives = 62/114 (54.39%), Query Frame = 3 
Query:   45 MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDN 386
            M++    +EQ YDRQ+RLWG   Q  L+NA   ++  +    E++K+L+L GI    + D   V  ED+ NNF +   SIG+NRA AA   ++ LN  V        P ++ DN
Sbjct:    1 MSEQKTNKEQRYDRQLRLWGDHGQEALENAHTCLLNASATGTEILKNLILPGIGAFTIVDGHKVTGEDVGNNFFLSCESIGKNRAQAATEFLQELNSDVSGNFVEESPDKLLDN 114
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Cavefish
Match: uba1 (ubiquitin like modifier activating enzyme 1 [Source:NCBI gene;Acc:103045813])

HSP 1 Score: 77.0258 bits (188), Expect = 1.115e-14
Identity = 52/187 (27.81%), Postives = 88/187 (47.06%), Query Frame = 3 
Query:   57 TEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKP 617
             E +E +Y RQ+ + G DA  R++++ VLI G+ GL  E+ K+++L G+  + L D       D+++ F +    +G+NRA  +++R+  LN  V V  Y     E+ D+      + +F +VV  +S I     +    + E C          D+ G  G +F D        E I     GE+P
Sbjct:   48 AEIDEGLYSRQLYVLGHDAMKRMQSSNVLISGLRGLGVEIAKNVILGGVKTVTLHDQGVAEWNDLSSQFYLREEDLGKNRAEVSQTRLAELNSYVPVTAYTG---ELTDDY-----LTDFQVVVLTSSTI-----DEQTRIGEFCHSKGIKLIIADTRGLFGQLFCDFG-----EEMIVYDTNGEQP 216
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Cavefish
Match: nae1 (NEDD8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:103042317])

HSP 1 Score: 59.6918 bits (143), Expect = 2.762e-9
Identity = 35/114 (30.70%), Postives = 54/114 (47.37%), Query Frame = 3 
Query:   45 MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDN 386
            M++    +EQ YDRQ+RLWG   Q  L+NA   ++  +    E++K+L+L GI    + D   V  ED+             N+A AA   ++ LN  V        P ++ DN
Sbjct:    2 MSEQKTNKEQRYDRQLRLWGDHGQEALENAHTCLLNASATGTEILKNLILPGIGAFTIVDGHKVTGEDVG------------NKAQAATEFLQELNSDVSGNFVEESPDKLLDN 103
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Cavefish
Match: uba2 (ubiquitin like modifier activating enzyme 2 [Source:NCBI gene;Acc:103037342])

HSP 1 Score: 54.6842 bits (130), Expect = 1.017e-7
Identity = 24/81 (29.63%), Postives = 47/81 (58.02%), Query Frame = 3 
Query:  117 NRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYN 359
            + + + K+L++G  G+ CE++K+LVLTG   I + D   ++  ++N  FL ++  +G+++A  AK  V    P   +  Y+
Sbjct:   14 DSVSSCKILVVGAGGIGCELLKNLVLTGFKNIEVIDLDTIDVSNLNRQFLFQKKHVGKSKAQVAKESVLRFCPSANITAYH 94
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Sea Lamprey
Match: sae1 (SUMO1 activating enzyme subunit 1 [Source:NCBI gene;Acc:415148])

HSP 1 Score: 178.333 bits (451), Expect = 6.277e-53
Identity = 112/343 (32.65%), Postives = 176/343 (51.31%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVRE---EIEMQKPGEKPANNPSKWIS-------EVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSET-HNYATGTDIIAIFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSIKPE 1049
            I+EEE   YDRQIRLWGL+AQ RL+ +K+LI+G+ GL  EV K+L+L G+  +   D+    EE     FL+ +S +GEN A A+  R +NLNP V V        +I        T  +FD +  C +  P    ++ + +N  C + N  FF  D FG++G MF DL  H+YV E   ++E++   + PA    K IS       + +EFC+  + L  DW+     + +K+ P   F+   +  F +E   + A  T      DT         L  ++     +  C    + P+ AV+GG++ QEI+KA+S ++ P+ NF  F+G++ + +   I  E
Sbjct:   12 ISEEEAAQYDRQIRLWGLEAQKRLRASKLLIVGMKGLGAEVAKNLILAGVKSVTFLDNNKATEESTRAQFLVPQSRLGENLAEASLERAQNLNPMVDVRTDKS---DIASKTEEFFT--QFDSI--CLTGCPQ---DVMIKINRICHERNIKFFAGDVFGYHGYMFADLGIHDYVEEKPKKVEVEGAEDGPAAKKRKVISSESTMVKKTVEFCTLVEALDADWTTDQAKKIIKKTPAGYFLTKVLLRFRTEQGRDPAPQTQTE---DTRHDSRRIRELGGDIFPHDFYSSCFSELS-PICAVVGGVLGQEIVKALSGRDEPSNNFFFFDGLAGNGMVDCIGKE 340
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Sea Lamprey
Match: nae1 (nedd8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:573336])

HSP 1 Score: 90.1225 bits (222), Expect = 6.501e-20
Identity = 58/191 (30.37%), Postives = 92/191 (48.17%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVR-------EEIEMQKP 605
            + +E+E+ YDRQ+RLWG   Q  L+NA V +I  +    E++K+LVL GI    + D   V+ EDI NNF +++SSIGE+RA  A + ++ LN     +V  +   E  + +  K         V  T+ +P    +  L L     +         ++G  G M V L  H  V        E++ + +P
Sbjct:    5 LAKEKERRYDRQLRLWGDHGQQALENAHVCLINASATGTEILKNLVLPGIGAFTILDGNKVSGEDIGNNFFLDKSSIGESRAKTAMNLIQELNS----DVSGNFVEEAVEKVLEKEPTFFHKFTVVITTQLPQ---STLLRLAAALWEAGVPLLACRAYGMTGYMRVALREHTVVESHPDNALEDLRLDRP 188
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Sea Lamprey
Match: uba3 (ubiquitin-like modifier activating enzyme 3 [Source:NCBI gene;Acc:406776])

HSP 1 Score: 56.225 bits (134), Expect = 5.470e-9
Identity = 34/105 (32.38%), Postives = 56/105 (53.33%), Query Frame = 3 
Query:  123 LKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNIN--NKLTIEEFDIVVC 431
            L++ K+L++G  GL CE++KDL L+G  +I + D   ++  ++N  FL     +G  +A  A    E +N R  V   N MP+    NI   ++    +F I+VC
Sbjct:   53 LESCKILVLGAGGLGCELLKDLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDVGRPKANVA---AEFINTR--VPGCNVMPYPSFSNIQEYDESFYRQFHIIVC 152
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Yeast
Match: AOS1 (Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000006384])

HSP 1 Score: 116.701 bits (291), Expect = 5.708e-30
Identity = 93/355 (26.20%), Postives = 172/355 (48.45%), Query Frame = 3 
Query:   45 MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICD-NINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQ--KPGEKPANNPSKWISEVL-------EFCSFDKV--------LQIDWSLAS-----HARKLKRIPKLQFIMLAMYNFVSETHNYATGTDIIAIFDTWQAFAV----KHGLDINLLD---ISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSC 1019
            + K++E+E  +YDRQIRLWG+ AQ  +++AKVL+I +  +  E+ K +VL+GI  + + D   V EED+ + F I    +G+ +  A K R+++LNPR+       + F+  D    ++   ++FD+VV     I        + +N   RK N   +   S G    +F+DL   E++ E+ ++Q  +P      + ++ I EV        E  +++++        L    S A+       R+LKR+  +  + L++  +       A        F+  +  A       G+   ++    I  F +  G+   PV A++GG V Q++I  + K+ +P  NF++F+G++ 
Sbjct:    5 VEKLSEDEIALYDRQIRLWGMTAQANMRSAKVLLINLGAIGSEITKSIVLSGIGHLTILDGHMVTEEDLGSQFFIGSEDVGQWKIDATKERIQDLNPRI------ELNFDKQDLQEKDEEFFQQFDLVVATEMQIDE-----AIKINTLTRKLNIPLYVAGSNGLFAYVFIDLI--EFISEDEKLQSVRPTTVGPISSNRSIIEVTTRKDEEDEKKTYERIKTKNCYRPLNEVLSTATLKEKMTQRQLKRVTSILPLTLSLLQYGLNQKGKAIS------FEQMKRDAAVWCENLGVPATVVKDDYIQQFIKQKGIEFAPVAAIIGGAVAQDVINILGKRLSPLNNFIVFDGITL 340
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Yeast
Match: UBA1 (Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000001693])

HSP 1 Score: 78.1814 bits (191), Expect = 4.969e-16
Identity = 48/186 (25.81%), Postives = 91/186 (48.92%), Query Frame = 3 
Query:   60 EEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKP 617
            E +E +Y RQ+ + G +A  +++ + VLI+G+ GL  E+ K++VL G+  + + D   V   D++  F +    IG+ R    ++++  LN         ++P  + D++++   + +F +VV   +   S +D   + +NE C      F   ++ G  G  FVDL     V     +   GE+P
Sbjct:   13 EIDESLYSRQLYVLGKEAMLKMQTSNVLILGLKGLGVEIAKNVVLAGVKSMTVFDPEPVQLADLSTQFFLTEKDIGQKRGDVTRAKLAELNA--------YVPVNVLDSLDDVTQLSQFQVVVATDTV--SLEDK--VKINEFCHSSGIRFISSETRGLFGNTFVDLGDEFTV-----LDPTGEEP 181

HSP 2 Score: 61.6178 bits (148), Expect = 1.018e-10
Identity = 30/100 (30.00%), Postives = 55/100 (55.00%), Query Frame = 3 
Query:   51 KITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINK-----IALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNP 335
            K T+     YD QI ++GLD Q ++ N+KV ++G   + CE++K+  L G+       I + D+ ++ + ++N  FL     +G+N++  A   V  +NP
Sbjct:  408 KTTQPVNSRYDNQIAVFGLDFQKKIANSKVFLVGSGAIGCEMLKNWALLGLGSGSDGYIVVTDNDSIEKSNLNRQFLFRPKDVGKNKSEVAAEAVCAMNP 507
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Yeast
Match: UBA4 (E1-like protein that activates Urm1p before urmylation; also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p [Source:SGD;Acc:S000001153])

HSP 1 Score: 58.9214 bits (141), Expect = 4.446e-10
Identity = 32/104 (30.77%), Postives = 54/104 (51.92%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQI---RLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVY 356
            ++ EE Q Y RQ+      G+  Q +LKN KVL++G  GL C  +  L   G+ +I + D+  V   +++   L + S +G  +  +A+  +  LNP + V  Y
Sbjct:   39 LSLEEYQRYGRQMIVEETGGVAGQVKLKNTKVLVVGAGGLGCPALPYLAGAGVGQIGIVDNDVVETSNLHRQVLHDSSRVGMLKCESARQYITKLNPHINVVTY 142
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Nematostella
Match: EDO49380 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG90])

HSP 1 Score: 178.333 bits (451), Expect = 1.424e-52
Identity = 118/342 (34.50%), Postives = 186/342 (54.39%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT--IEEFDIVVC--CTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPA-----------NNPSKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSETHNYATGTDIIAIFDTWQAFAVKHGL--DIN----LLDISDFE-ECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGV 1013
            ITE E  +YDRQIRLWGLDAQ RL+ +++L++G+ G+  E+ K+LVL+G+  + + D+  V E D  + FL  R ++G+NRA A+ +R + LNP V V           +NI  K    +++FD+VV   C+S       ++ +++ E+CR  N  FF  D FG+ G MF DL  H YV EE +     EK             +  +K + +  EF S    L   +S  +  + LKR+P + FI+  +  F ++ H  A  + +    D  +   +K  +  D+N    L+D  DF   C+   + PV A++GG+V QEI+KA+S K+AP  NF  ++G+
Sbjct:   10 ITEAEAALYDRQIRLWGLDAQKRLRASRILVVGLAGIGAEICKNLVLSGVKSLTMLDNNPVTERDFVSQFLAPREALGKNRAEASLARTQALNPMVAVSADK-------NNITAKADTFLDDFDVVVATGCSS-------DILVSIYERCRAKNIKFFASDVFGFYGYMFADLGKHRYVEEERKTIHSAEKKEKEPAKKKQKIDSTETKTVEKFCEFSSLKNSLSCSFS-ETRVKSLKRLPSVYFILQVILRFRAK-HGRAPDS-LQRTSDEKELNCLKQEVMSDLNISQDLID-QDFACHCLSELS-PVCAIVGGVVGQEIVKAVSGKDAPLNNFFFYDGL 332
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Nematostella
Match: EDO44524 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RV71])

HSP 1 Score: 78.1814 bits (191), Expect = 1.031e-15
Identity = 53/194 (27.32%), Postives = 86/194 (44.33%), Query Frame = 3 
Query:   45 MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVR-------EEIEMQKP 605
            M K   E+E+ YDRQ+RLWG   Q  L+ AK+ +I  +    E++K+L+L GI    + D   V  ED+ NNF + +  IG +RA  A   +  LN  V  E     P E+  +  +    + F +V+          + + L L               S+G+ G M + L  H  +        E++ + +P
Sbjct:    1 MPKTAVEKEKKYDRQLRLWGDHGQAALEQAKICLINASATGTEILKNLILPGIGLFTIVDGEKVTGEDVGNNFFLNKEHIGRSRAECATELLLELNSDVSGEFIEESPEELLTHKQD--FFKPFSVVIAT-----QLSEEILLKLAHVLWSDKVPLLVCRSYGFIGYMRLVLEEHPIIESHPDSAFEDLRLDRP 187
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Nematostella
Match: EDO33332 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSG6])

HSP 1 Score: 72.4034 bits (176), Expect = 1.054e-13
Identity = 53/190 (27.89%), Postives = 88/190 (46.32%), Query Frame = 3 
Query:   66 EEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLT---IEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANN 626
            +E +Y RQ+ + G +A  ++  + VLI G+ GL  E+ K++VL G+  + L D   V   D+ + F + +  +G+NRA  +  R+  LN  V + V              KLT   I +F +VV   S   S ++ LW  +++ C          D+ G  G +F D      V     +   GE+P +N
Sbjct:    4 DEGLYSRQLYVLGHEAMKKMAVSNVLISGLRGLGVEIAKNVVLGGVKSVTLHDTGAVELSDLTSQFFLNKKDVGKNRAEVSHPRIAELNTYVSMSVNTQ-----------KLTEEFINKFQVVVLTES---SLEEQLW--ISDFCHSKGIKLIISDTKGLFGQIFCDFGESFTV-----VDTNGEQPVSN 172

HSP 2 Score: 47.7506 bits (112), Expect = 6.674e-6
Identity = 30/125 (24.00%), Postives = 55/125 (44.00%), Query Frame = 3 
Query:   78 YDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINK-----IALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIV------------EVYNHMPFEICDNINNKL 401
            YD QI ++G + Q +L   K  ++G   + CE +K+  + G+       I   D   + + ++N  FL   + + + ++  A   V+ +NP V +            +VYN   FE    + N L
Sbjct:  401 YDGQIAVFGSEFQKKLGQQKYFVVGAGAIGCEHLKNFAMMGLGSGEGGHIYTTDMDTIEKSNLNRQFLFRPADVQKMKSEVAARAVKVMNPDVNITAHGNRVGPETEKVYNDDFFESLSGVTNAL 525
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Nematostella
Match: EDO38775 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBV9])

HSP 1 Score: 64.6994 bits (156), Expect = 2.859e-11
Identity = 49/178 (27.53%), Postives = 78/178 (43.82%), Query Frame = 3 
Query:   96 LWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSI--GENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCR--KYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKP 617
            + G  A  ++ +A V + G+ GL  E+ K+L L GI  I L D    +  D+ + F +    +    NRA A+  RV  LNP V V    H   +  D  NN   ++ +  V+   + +     ++ L +N  CR  K  K F     +G  G +F D  +   V     +   GE+P
Sbjct:    1 MLGDQAMQKMAHASVFLSGVGGLGVEIAKNLTLAGIKSITLHDTRAASMADLGSQFFLREDDVTSSRNRAVASAGRVAELNPYVSV----HTQTDALDE-NNLDVLKNYQCVILTDAPL-----SVQLKVNSYCRSQKPQKQFISTSLYGIFGCLFCDFGNEFEV-----LDTTGEEP 163
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Nematostella
Match: EDO46454 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPW5])

HSP 1 Score: 57.3806 bits (137), Expect = 6.447e-9
Identity = 26/84 (30.95%), Postives = 52/84 (61.90%), Query Frame = 3 
Query:  117 NRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRV-IVEVYNHM 365
            +++ N+K+L++G  G+ CE++K+LVLTG   I L D   ++  ++N  FL  +  +G+++A  A+      NP   IV +++++
Sbjct:   19 DKVNNSKILVVGAGGIGCELLKNLVLTGFKNIDLIDLDTIDVSNLNRQFLFHKQHVGKSKAKVARESALRFNPDAKIVAIHDNI 102
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Medaka
Match: sae1 (SUMO1 activating enzyme subunit 1 [Source:NCBI gene;Acc:101162772])

HSP 1 Score: 153.295 bits (386), Expect = 1.105e-42
Identity = 105/335 (31.34%), Postives = 172/335 (51.34%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAH-HEYVREEIEMQKPGEKPANNP-----------SKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSETHN----YATGTDIIAIFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNG 1010
            I+EEE   YDRQIRLWGLDAQ RL+ ++VL+ G+ GL  EV K+L+L G+  + L D   V EE     FL+  S+ G+NRA A+  R +NLNP  +VEV+   P  + D  ++     +FD  VC T        +L + +++ C ++    F  D +G+ G MF +L   + YV E+ ++ KP    ++ P           +  + +   FC+  + L++DW+       LK+ P   F++  +  F ++        ++  D   +           GL  +LL+      C    + PV AV+GG++ QE++KA+S+++ P  NF  F+G
Sbjct:    9 ISEEEAAQYDRQIRLWGLDAQKRLRGSRVLLAGLGGLGAEVAKNLILAGVKGLTLLDHEQVTEESCRAQFLVPVSARGQNRAQASLERAQNLNP--MVEVHAD-PGRVEDKPDDFFL--QFD-AVCLT----GCSRDLMVRVDQLCSQHRIKVFCGDVYGYYGYMFCNLGQEYSYVEEKPKVVKPSGDSSDGPGAKKAKVDPNETTMVKKTASFCTLKEALEVDWTTEKAKAALKKTPVDYFLLHVLLKFRTDKGRDPDPQSSEEDGELLRQIRSDVLDALGLSASLLNDDFISYCFSEMS-PVCAVVGGVLGQEVVKALSQRDVPHRNFFFFDG 332
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Medaka
Match: nae1 (NEDD8 activating enzyme E1 subunit 1 [Source:NCBI gene;Acc:101170334])

HSP 1 Score: 83.9593 bits (206), Expect = 3.616e-17
Identity = 45/114 (39.47%), Postives = 60/114 (52.63%), Query Frame = 3 
Query:   45 MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDN 386
            M      +EQ YDRQ+RLWG   Q  L+NA V +I       E++K+LVL GI    + D   V+ ED  NNF +  +SIG NRA AA   ++ LN  V        P ++ DN
Sbjct:    1 MAATKASKEQKYDRQLRLWGDHGQEALENAHVCLINATATGTEILKNLVLPGIGAFTIVDGHTVSGEDAGNNFFLSNNSIGRNRAQAATELLQELNGDVSGNFVEESPDKLLDN 114
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Medaka
Match: sae1 (SUMO1 activating enzyme subunit 1 [Source:NCBI gene;Acc:101162772])

HSP 1 Score: 75.485 bits (184), Expect = 5.101e-15
Identity = 48/198 (24.24%), Postives = 95/198 (47.98%), Query Frame = 3 
Query:  462 NLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAH-HEYVREEIEMQKPGEKPANNPSKWISEV----------LEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSETHN----YATGTDIIAIFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNG 1010
            +L + +++ C ++    F  D +G+ G MF +L   + YV E+ ++ KP    ++ P    ++V          ++FC+  + L++DW+       LK+ P   F++  +  F ++        ++  D   +           GL  +LL+      C    + PV AV+GG++ QE++KA+S+++ P  NF  F+G
Sbjct:   77 DLMVRVDQLCSQHRIKVFCGDVYGYYGYMFCNLGQEYSYVEEKPKVVKPSGDSSDGPGAKKAKVDPNETTMVKKVDFCTLKEALEVDWTTEKAKAALKKTPVDYFLLHVLLKFRTDKGRDPDPQSSEEDGELLRQIRSDVLDALGLSASLLNDDFISYCFSEMS-PVCAVVGGVLGQEVVKALSQRDVPHRNFFFFDG 273

HSP 2 Score: 59.6918 bits (143), Expect = 1.252e-9
Identity = 32/57 (56.14%), Postives = 42/57 (73.68%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCD 224
            I+EEE   YDRQIRLWGLDAQ RL+ ++VL+ G+ GL  EV K+L+L G+  + L D
Sbjct:    9 ISEEEAAQYDRQIRLWGLDAQKRLRGSRVLLAGLGGLGAEVAKNLILAGVKGLTLLD 65
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Medaka
Match: uba7 (ubiquitin-like modifier-activating enzyme 1 [Source:NCBI gene;Acc:101175149])

HSP 1 Score: 73.559 bits (179), Expect = 1.469e-13
Identity = 50/190 (26.32%), Postives = 92/190 (48.42%), Query Frame = 3 
Query:   57 TEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIE-MQKPGEKPAN 623
            +E +E  Y RQ+ + G +A  R+  A VLI G+ GL  E+ K+++L+G+  + + D+ +    D+++ F +    +G+NRA  +  ++ +LNP V V  +          ++ +L +  F IVV   S +   K        + C  +       D+ G  G +F D        E+ E +++ GE PA+
Sbjct:    5 SEIDEGFYSRQLYVLGHEAMRRMGAANVLIAGLKGLGVEIAKNVILSGVKAVTVQDEGSAVWSDLSSQFYLGECHLGQNRASCSLQQLSSLNPHVEVSAHT-------GPLDEELLL-RFQIVVLTDSSLDDQK-----RFGQLCHSHGIKLIVADTKGLCGQLFCDFG------EKFEVLERDGEMPAS 175
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Medaka
Match: uba7 (ubiquitin-like modifier-activating enzyme 1 [Source:NCBI gene;Acc:101175149])

HSP 1 Score: 73.1738 bits (178), Expect = 1.528e-13
Identity = 50/190 (26.32%), Postives = 92/190 (48.42%), Query Frame = 3 
Query:   57 TEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIE-MQKPGEKPAN 623
            +E +E  Y RQ+ + G +A  R+  A VLI G+ GL  E+ K+++L+G+  + + D+ +    D+++ F +    +G+NRA  +  ++ +LNP V V  +          ++ +L +  F IVV   S +   K        + C  +       D+ G  G +F D        E+ E +++ GE PA+
Sbjct:    5 SEIDEGFYSRQLYVLGHEAMRRMGAANVLIAGLKGLGVEIAKNVILSGVKAVTVQDEGSAVWSDLSSQFYLGECHLGQNRASCSLQQLSSLNPHVEVSAHT-------GPLDEELLL-RFQIVVLTDSSLDDQK-----RFGQLCHSHGIKLIVADTKGLCGQLFCDFG------EKFEVLERDGEMPAS 175
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Planmine SMEST
Match: SMESG000036431.1 (SMESG000036431.1)

HSP 1 Score: 683.715 bits (1763), Expect = 0.000e+0
Identity = 333/335 (99.40%), Postives = 334/335 (99.70%), Query Frame = 3 
Query:   45 MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNPSKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSETHNYATGTDIIAIFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSIKPE 1049
            MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIK+LVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNPSKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSETHNYATGTDI AIFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSIKPE
Sbjct:    1 MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKNLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNPSKWISEVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSETHNYATGTDISAIFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVTQEIIKAISKKNAPTGNFVLFNGVSCSAVSTSIKPE 335
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Planmine SMEST
Match: SMESG000016314.1 (SMESG000016314.1)

HSP 1 Score: 85.8853 bits (211), Expect = 6.045e-18
Identity = 62/203 (30.54%), Postives = 95/203 (46.80%), Query Frame = 3 
Query:   54 ITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNPSKWISEVLEFC 662
            +T E+ + YDRQIRLWG + Q  L+NAKV +IG   +  E++K+L+L GI    + DD  V EED++NNF +++S + ++RA      +  LN  VIV         +     N     EF +V+ C        +   ++L+      N       S G    + V +  H  V    E +    KP     K + E +EFC
Sbjct:    3 LTLEKTKRYDRQIRLWGENGQIALENAKVCLIGSGCIGTEILKNLILPGIGSFTIVDDSVVTEEDLSNNFFVQKSDLNQSRAEVTARLLRELNEEVIVNYIEDSVESLM--FRNPEYFLEFSVVIAC-----GLNNKDLVDLDRLLYPENIGLIVCHSIGMVANLRVIIQEHPVV----EAKPDNIKPDLRLDKPLWEFVEFC 194
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Planmine SMEST
Match: SMESG000041261.1 (SMESG000041261.1)

HSP 1 Score: 84.3445 bits (207), Expect = 3.573e-17
Identity = 53/187 (28.34%), Postives = 95/187 (50.80%), Query Frame = 3 
Query:   66 EEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKP-GEKPAN 623
            +E +Y RQ+ + G +    + ++ VLI G+ GL  E+ K++VL G+  + L D   V+E+D+++ + + +  I +NRA A+K  +  LN        +++P  + +      T+++F IVV   S +   K     N+N  C + N  F   ++FG  G +F D        EE  +  P GE+P +
Sbjct:   10 DENLYSRQLYVMGKEGMRNMASSSVLISGLGGLGVEIAKNIVLAGVKSVTLHDTKKVSEDDLSSQYFLRKEHIKQNRATASKLSLSELN--------SYVPIYVIERDLTLETLKQFTIVVLTESSLDEQK-----NINRFCHENNIKFLNANTFGLFGQIFCDFG------EEFIINDPSGEEPQS 177

HSP 2 Score: 50.0618 bits (118), Expect = 2.430e-6
Identity = 31/160 (19.38%), Postives = 73/160 (45.62%), Query Frame = 3 
Query:   78 YDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGI-----NKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNG 542
            Y  QI ++G + Q +L N    ++G   + CE +K+  + GI      KI + D  ++ + ++N  FL     I + ++  A   ++ +NP + +E + +    +C++  +  + + F+ +    + + +    ++  ++  C  Y K      + G  G
Sbjct:  412 YSGQIAIFGSEFQKKLSNLNYFVVGSGAIGCEHLKNFAMMGIGSGPRGKIVVTDMDSIEKSNLNRQFLFRSHDIQKMKSEVAARSIKTMNPEINIESHVN---RVCEDTEHVYSEKFFNSIDGVANALDNVNARMY--MDRLCVFYRKPLLESGTLGTKG 566
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Planmine SMEST
Match: SMESG000070068.1 (SMESG000070068.1)

HSP 1 Score: 76.6406 bits (187), Expect = 8.567e-15
Identity = 62/206 (30.10%), Postives = 102/206 (49.51%), Query Frame = 3 
Query:   60 EEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLW-LNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNPSKWISEVLEFCSFDK 674
            E +E +Y RQ+ + G  A   +   ++LI GINGL  EV K+++L+G++++ L D  ++   D+++ F    S IG NRA      +  +N  V V+        + + ++ KL I ++D+ V C S     K N+    +NE CRK +  F    + G  G +F D    +YV  +I   +P     N+  K   E L + S DK
Sbjct:    4 EIDEGLYSRQLYVLGKKAMQEISRKRILITGINGLGLEVAKNVILSGVSQVTLHDTQSITSSDLSSYFYGNISDIGRNRAEVCHHHLCEINEYVKVDW-------LKEQLSEKL-ISQYDVAVLCDS-----KSNVKNKEINEWCRKSHVKFILGSATGLFGSIFCDFG-DDYVVNDISGTEPEAIEINSIEK-SKEGLVYVSGDK 194

HSP 2 Score: 55.8398 bits (133), Expect = 3.874e-8
Identity = 36/123 (29.27%), Postives = 60/123 (48.78%), Query Frame = 3 
Query:   78 YDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGI-----NKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVY-NHMPFEICDNINNKLTIEEFDIVV 428
            YD QI ++G   Q +L+N K  +IG   + CE++K+  + G+      KI + D   +   ++N  FL     IG++++  A   V  +N  + +E + N +  E  D  NNK      D VV
Sbjct:  410 YDGQIAVFGSQFQKKLENLKYFLIGAGAIGCEMLKNFAMIGVGTGADGKIFVTDMDFIERSNLNRQFLFRNWDIGQSKSLTATKAVLKMNQSLHIEAHENRLGQETEDFYNNKF-FRSLDCVV 531
[Close]
BLAST of SUMO-activating enzyme subunit 1 vs. Planmine SMEST
Match: SMESG000081278.1 (SMESG000081278.1)

HSP 1 Score: 68.1662 bits (165), Expect = 4.833e-12
Identity = 54/195 (27.69%), Postives = 92/195 (47.18%), Query Frame = 3 
Query:   42 IMNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVLTGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVIVEVYNHMPFEICDNINNKLTIE---EFDIVVCCTSYIPSFKDNLWLNLNEKCRKYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKP 617
            I+N I E +E +Y RQ+ + G +A   +  + +LI G+ GL  E+ K+++L G+  + L D+L+  E  +++ + +  ++IG+NRA +    +  LN  V V V             N LT E    F+IVV     + + K +  +N+   C   N       +FG  G +F D      V +       GE+P
Sbjct:   37 IINGI-EIDENLYSRQLYVMGKEAMKNMSKSNILISGMGGLGVEIAKNVILAGVKSVTLHDELSATECSLSSQYYLNENNIGQNRATSCFKHLAELNSYVNVNV-----------DTNPLTKEMLLNFNIVV-----LTNIKQDEIINITGFCHLNNIKLIITRTFGLFGQIFCDFGPSHLVTDST-----GEEP 209
The following BLAST results are available for this feature:
BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SAE17.605e-5130.17SUMO1 activating enzyme subunit 1 [Source:HGNC Sym... [more]
SAE11.452e-4032.42SUMO1 activating enzyme subunit 1 [Source:HGNC Sym... [more]
SAE11.111e-3933.20SUMO1 activating enzyme subunit 1 [Source:HGNC Sym... [more]
SAE12.533e-3933.20SUMO1 activating enzyme subunit 1 [Source:HGNC Sym... [more]
SAE11.533e-3636.73SUMO1 activating enzyme subunit 1 [Source:HGNC Sym... [more]
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BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
aos-11.835e-3431.27SUMO-activating enzyme subunit aos-1 [Source:UniP... [more]
ula-15.291e-1633.61NEDD8-activating enzyme E1 regulatory subunit [So... [more]
ula-15.822e-1633.61NEDD8-activating enzyme E1 regulatory subunit [So... [more]
uba-12.089e-825.27UBA (Human ubiquitin) related [Source:UniProtKB/T... [more]
uba-12.164e-825.27UBA (Human ubiquitin) related [Source:UniProtKB/T... [more]
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BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Aos16.404e-3630.68gene:FBgn0029512 transcript:FBtr0082593[more]
APP-BP15.117e-1630.49gene:FBgn0261112 transcript:FBtr0076208[more]
APP-BP15.117e-1630.49gene:FBgn0261112 transcript:FBtr0331179[more]
Uba44.146e-1125.43gene:FBgn0032054 transcript:FBtr0079683[more]
Uba44.146e-1125.43gene:FBgn0032054 transcript:FBtr0333414[more]
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BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sae11.119e-4932.84SUMO1 activating enzyme subunit 1 [Source:NCBI gen... [more]
nae11.248e-1739.47nedd8 activating enzyme E1 subunit 1 [Source:NCBI ... [more]
nae11.248e-1739.47nedd8 activating enzyme E1 subunit 1 [Source:NCBI ... [more]
uba71.065e-1327.81ubiquitin-like modifier activating enzyme 7 [Sourc... [more]
uba71.065e-1327.81ubiquitin-like modifier activating enzyme 7 [Sourc... [more]
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BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sae12.304e-5835.23SUMO1 activating enzyme subunit 1 [Source:NCBI gen... [more]
gng24.573e-1630.99guanine nucleotide binding protein (G protein), ga... [more]
igsf9b5.137e-929.75immunoglobulin superfamily member 9B [Source:Xenba... [more]
gng27.190e-826.40guanine nucleotide binding protein (G protein), ga... [more]
gng27.347e-839.44guanine nucleotide binding protein (G protein), ga... [more]
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BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Sae11.590e-5030.00SUMO1 activating enzyme subunit 1 [Source:MGI Symb... [more]
Sae11.590e-5030.00SUMO1 activating enzyme subunit 1 [Source:MGI Symb... [more]
Sae13.514e-4230.00SUMO1 activating enzyme subunit 1 [Source:MGI Symb... [more]
Uba74.727e-1729.78ubiquitin-like modifier activating enzyme 7 [Sourc... [more]
Nae17.859e-1737.68NEDD8 activating enzyme E1 subunit 1 [Source:MGI S... [more]
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BLAST of SUMO-activating enzyme subunit 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q28DS0|SAE1_XENTR1.221e-5735.23SUMO-activating enzyme subunit 1 OS=Xenopus tropic... [more]
sp|Q8JGT5|SAE1_XENLA1.485e-5734.67SUMO-activating enzyme subunit 1 OS=Xenopus laevis... [more]
sp|A2VE14|SAE1_BOVIN1.308e-5131.43SUMO-activating enzyme subunit 1 OS=Bos taurus OX=... [more]
sp|Q6AXQ0|SAE1_RAT7.827e-5130.57SUMO-activating enzyme subunit 1 OS=Rattus norvegi... [more]
sp|Q5NVN7|SAE1_PONAB3.652e-5030.17SUMO-activating enzyme subunit 1 OS=Pongo abelii O... [more]
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BLAST of SUMO-activating enzyme subunit 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0L8H8Z84.332e-5934.55ThiF domain-containing protein OS=Octopus bimaculo... [more]
A0A1S3IQB34.794e-5737.20SUMO-activating enzyme subunit 1 isoform X2 OS=Lin... [more]
A0A1S3IQQ46.463e-5737.20SUMO-activating enzyme subunit 1 isoform X1 OS=Lin... [more]
A0A401RNN68.705e-5733.71ThiF domain-containing protein OS=Chiloscyllium pu... [more]
A0A2C9JTP82.596e-5638.58ThiF domain-containing protein OS=Biomphalaria gla... [more]
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BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sae13.956e-2330.62SUMO1 activating enzyme subunit 1 [Source:NCBI gen... [more]
nae11.595e-1636.84NEDD8 activating enzyme E1 subunit 1 [Source:NCBI ... [more]
uba11.115e-1427.81ubiquitin like modifier activating enzyme 1 [Sourc... [more]
nae12.762e-930.70NEDD8 activating enzyme E1 subunit 1 [Source:NCBI ... [more]
uba21.017e-729.63ubiquitin like modifier activating enzyme 2 [Sourc... [more]
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BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
sae16.277e-5332.65SUMO1 activating enzyme subunit 1 [Source:NCBI gen... [more]
nae16.501e-2030.37nedd8 activating enzyme E1 subunit 1 [Source:NCBI ... [more]
uba35.470e-932.38ubiquitin-like modifier activating enzyme 3 [Sourc... [more]
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BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
AOS15.708e-3026.20Subunit of heterodimeric nuclear SUMO activating e... [more]
UBA14.969e-1625.81Ubiquitin activating enzyme (E1); involved in ubiq... [more]
UBA44.446e-1030.77E1-like protein that activates Urm1p before urmyla... [more]
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BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO493801.424e-5234.50Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO445241.031e-1527.32Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO333321.054e-1327.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO387752.859e-1127.53Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO464546.447e-930.95Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SUMO-activating enzyme subunit 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sae11.105e-4231.34SUMO1 activating enzyme subunit 1 [Source:NCBI gen... [more]
nae13.616e-1739.47NEDD8 activating enzyme E1 subunit 1 [Source:NCBI ... [more]
sae15.101e-1524.24SUMO1 activating enzyme subunit 1 [Source:NCBI gen... [more]
uba71.469e-1326.32ubiquitin-like modifier-activating enzyme 1 [Sourc... [more]
uba71.528e-1326.32ubiquitin-like modifier-activating enzyme 1 [Sourc... [more]
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BLAST of SUMO-activating enzyme subunit 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000036431.10.000e+099.40SMESG000036431.1[more]
SMESG000016314.16.045e-1830.54SMESG000016314.1[more]
SMESG000041261.13.573e-1728.34SMESG000041261.1[more]
SMESG000070068.18.567e-1530.10SMESG000070068.1[more]
SMESG000081278.14.833e-1227.69SMESG000081278.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034728 ID=SMED30034728|Name=SUMO-activating enzyme subunit 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1213bp
CTTCCGATCTAAATAATATATTTATTAACCATTAGTACCATATTATGAAC
AAGATTACTGAAGAAGAAGAGCAAGTATACGACCGCCAGATCAGATTGTG
GGGCTTAGACGCCCAAAATAGATTAAAAAATGCTAAAGTTCTTATTATAG
GAATAAATGGACTGTCCTGTGAAGTAATAAAAGATTTAGTATTAACTGGT
ATCAACAAAATAGCATTGTGCGATGACTTAAATGTTAACGAAGAAGATAT
TAATAATAATTTTTTAATTGAACGTTCATCTATTGGTGAAAATAGAGCAT
ATGCAGCCAAAAGCAGAGTCGAAAATTTAAATCCTAGAGTTATAGTTGAA
GTTTATAATCATATGCCATTTGAAATTTGCGACAACATAAATAACAAATT
AACTATTGAAGAATTCGATATAGTTGTTTGCTGTACATCATATATTCCAT
CTTTTAAAGATAATTTGTGGTTAAATCTCAACGAAAAATGTAGAAAATAC
AATAAATCTTTTTTTGGAATGGATTCGTTTGGTTGGAATGGAATTATGTT
TGTAGATTTAGCACACCATGAATATGTGCGAGAGGAAATTGAAATGCAAA
AACCAGGAGAAAAACCAGCTAATAATCCATCCAAATGGATATCTGAAGTC
CTAGAATTCTGTTCATTTGATAAAGTTTTACAAATCGATTGGAGTCTCGC
AAGTCATGCAAGGAAATTGAAAAGGATTCCTAAATTGCAATTTATAATGC
TTGCTATGTATAACTTTGTGTCCGAAACTCATAATTATGCCACTGGGACC
GATATCATCGCCATTTTTGATACTTGGCAAGCCTTTGCTGTCAAACATGG
ATTAGATATTAATCTATTGGATATATCTGATTTCGAGGAGTGCATCGGAG
TCGGTAATGTACCCGTAAATGCAGTACTTGGAGGCCTTGTAACCCAGGAG
ATCATTAAGGCTATTTCGAAGAAAAATGCGCCCACTGGAAATTTTGTCCT
TTTCAATGGCGTTTCTTGTTCAGCTGTTTCGACATCTATCAAACCGGAAT
GAGGATATTCCGGTTTCCTTGCTGTTTATAAATTGCATTTATGTGAATAA
AATATTTTTGGCTTATTTCATAGATTTTTGTTGAACATCGTAATTTATAT
TTTTAAGTTTCAGATTGTGAATTCTCACAGTTGCGGACATGCTAGAATAA
ATCCGTGTAAATA
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protein sequence of SMED30034728-orf-1

>SMED30034728-orf-1 ID=SMED30034728-orf-1|Name=SMED30034728-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=336bp
MNKITEEEEQVYDRQIRLWGLDAQNRLKNAKVLIIGINGLSCEVIKDLVL
TGINKIALCDDLNVNEEDINNNFLIERSSIGENRAYAAKSRVENLNPRVI
VEVYNHMPFEICDNINNKLTIEEFDIVVCCTSYIPSFKDNLWLNLNEKCR
KYNKSFFGMDSFGWNGIMFVDLAHHEYVREEIEMQKPGEKPANNPSKWIS
EVLEFCSFDKVLQIDWSLASHARKLKRIPKLQFIMLAMYNFVSETHNYAT
GTDIIAIFDTWQAFAVKHGLDINLLDISDFEECIGVGNVPVNAVLGGLVT
QEIIKAISKKNAPTGNFVLFNGVSCSAVSTSIKPE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0031510SUMO activating enzyme complex
GO:0005634nucleus
Vocabulary: biological process
TermDefinition
GO:0060216definitive hemopoiesis
GO:0061484hematopoietic stem cell homeostasis
GO:0006464cellular protein modification process
GO:0016925protein sumoylation
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002056medial muscle cell
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR035985Ubiquitin-activating_enz
IPR000594ThiF_NAD_FAD-bd
IPR016040NAD(P)-bd_dom
Vocabulary: molecular function
TermDefinition
GO:0008641small protein activating enzyme activity
GO:0008022protein C-terminus binding
GO:0016874ligase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.720coord: 1..335
e-value: 2.2E-86
score: 292.1
NoneNo IPR availablePANTHERPTHR10953:SF162SUMO-ACTIVATING ENZYME SUBUNIT 1coord: 6..326
NoneNo IPR availablePANTHERPTHR10953UBIQUITIN-ACTIVATING ENZYME E1coord: 6..326
NoneNo IPR availableCDDcd17041Ubl_WDR48coord: 176..219
e-value: 0.00152505
score: 35.3372
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 12..321
e-value: 8.0E-31
score: 107.3
IPR035985Ubiquitin-activating enzymeSUPERFAMILYSSF69572Activating enzymes of the ubiquitin-like proteinscoord: 5..328