DNA polymerase eta
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30034709 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of DNA polymerase eta vs. Ensembl Human
Match: POLH (DNA polymerase eta [Source:HGNC Symbol;Acc:HGNC:9181]) HSP 1 Score: 331.643 bits (849), Expect = 3.349e-103 Identity = 200/495 (40.40%), Postives = 275/495 (55.56%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSL------------TDLGELAMLKDRRDRSI--EGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQM-AAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNS 1510
++ L+DMDCF+VQVE + P K AVVQY +W+GGG IIAVSYEARA GV RSM D+AK+ CPD+ L V E+RGKA+LTKYR A EV++ +S+ A +ERASIDEA++D+T V L L T + L + ++ EG G WLD L T D++L +GA +V++MR + G++CSAGI+H+K AK ACG++KPN+QTLV + L + I KIR+LGGK+G V + L + MG+L+ F+ QL FG+K G WLY +CRG + + V RQL ++IGCSK FPG L T ++V++WL Q+AQE+ ER+++D + N R+ T + VS +R D R S S R L A +S AF V+ NT+G W PPL L L A KF ++ DI+ FL P +S VP+ S + S
Sbjct: 8 VVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGG--------------IIAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVMEIMSRFA-VIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYIEGLPQGPTTAEETVQKEGMRKQGLF---QWLDSLQIDNLTS---PDLQLTVGAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERLTKDRNDNDRVATQLVVS---IRVQGDKRLS-SLRRCCALTRYDAHKMSHDAFTVIKNCNTSG----IQTEWSPPLTMLFLCATKFSASAPSSSTDITSFLSSDP-------SSLPKVPVTSSEAKTQGS 466
BLAST of DNA polymerase eta vs. Ensembl Human
Match: POLH (DNA polymerase eta [Source:HGNC Symbol;Acc:HGNC:9181]) HSP 1 Score: 313.923 bits (803), Expect = 4.748e-100 Identity = 180/428 (42.06%), Postives = 251/428 (58.64%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSL------------TDLGELAMLKDRRDRSI--EGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAG 1312
++ L+DMDCF+VQVE + P K AVVQY +W+GGG IIAVSYEARA GV RSM D+AK+ CPD+ L V E+RGKA+LTKYR A EV++ +S+ A +ERASIDEA++D+T V L L T + L + ++ EG G WLD L+ + D++L +GA +V++MR + G++CSAGI+H+K AK ACG++KPN+QTLV + L + I KIR+LGGK+G V + L + MG+L+ F+ QL FG+K G WLY +CRG + + V RQL ++IGCSK FPG L T ++V++WL Q+AQE+ ER+++D + N R+ T + VS +R D R S S R L A +S AF V+ NT+G
Sbjct: 8 VVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGG--------------IIAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVMEIMSRFA-VIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYIEGLPQGPTTAEETVQKEGMRKQGLF---QWLD---SLQIDNLTSPDLQLTVGAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERLTKDRNDNDRVATQLVVS---IRVQGDKRLS-SLRRCCALTRYDAHKMSHDAFTVIKNCNTSG 410
BLAST of DNA polymerase eta vs. Ensembl Human
Match: POLI (DNA polymerase iota [Source:HGNC Symbol;Acc:HGNC:9182]) HSP 1 Score: 120.168 bits (300), Expect = 1.743e-27 Identity = 92/329 (27.96%), Postives = 155/329 (47.11%), Query Frame = 2
Query: 62 SNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNAS-IDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRF 1045
S+ +IV +D+DCFY QVE+ + P+ K V Q +++ ++ +YEAR GV + M +AK+KCP + L + DLT+YR +V + L + + VER DE F+D+T+ V+ L L + + G+V Y++ +L+++ +RL +G+ + +MRE +YN LG AG+A +K AK GV KPNQQT++ + L+++ + I +I +G K + + L ++++ L FS L G + L G+D+ V+ QS F
Sbjct: 51 SSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQ--------------KYL-----VVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVN------GEDLTRYREMSYKVTELLEEFSPVVERLGFDENFVDLTEMVEKRLQQL------------QSDELSAVTVSGHV-YNNQSINLLDVL-------HIRLLVGSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKCL-EALGINSVRDLQTFSPKILEKELGISVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSF 333
BLAST of DNA polymerase eta vs. Ensembl Human
Match: POLI (DNA polymerase iota [Source:HGNC Symbol;Acc:HGNC:9182]) HSP 1 Score: 97.4413 bits (241), Expect = 2.367e-22 Identity = 69/236 (29.24%), Postives = 119/236 (50.42%), Query Frame = 2
Query: 212 IQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNAS-IDKIRNLGGKMGTFVQQQLNVSTMGQLSAFS 916
+Q ++ +YEAR GV + M +AK+KCP + L + DLT+YR +V + L + + VER DE F+D+T+ V+ L L + + G+V Y++ +L+++ +RL +G+ + +MRE +YN LG AG+A +K AK GV KPNQQT++ + L+++ + I +I +G K + + L ++++ L FS
Sbjct: 14 VQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVN------GEDLTRYREMSYKVTELLEEFSPVVERLGFDENFVDLTEMVEKRLQQL------------QSDELSAVTVSGHV-YNNQSINLLDVL-------HIRLLVGSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKCL-EALGINSVRDLQTFS 222
BLAST of DNA polymerase eta vs. Ensembl Human
Match: POLI (DNA polymerase iota [Source:HGNC Symbol;Acc:HGNC:9182]) HSP 1 Score: 94.7449 bits (234), Expect = 1.654e-19 Identity = 82/315 (26.03%), Postives = 143/315 (45.40%), Query Frame = 2
Query: 62 SNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNAS-IDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVL 1003
S+ +IV +D+DCFY QVE+ + P+ K V Q +++ ++ +YEAR GV + M +AK+KCP + L + DLT+YR +V + + K ++ +DE+ + VS G+V Y++ +L+++ +RL +G+ + +MRE +YN LG AG+A +K AK GV KPNQQT++ + L+++ + I +I +G K + + L ++++ L FS L G + L G+D+ V+
Sbjct: 12 SSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQ--------------KYL-----VVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVN------GEDLTRYREMSYKVTEMVEKRLQQLQ-----------SDELSAVTVS------------------------GHV-YNNQSINLLDVL-------HIRLLVGSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKCL-EALGINSVRDLQTFSPKILEKELGISVAQRIQKLSFGEDNSPVI 257
BLAST of DNA polymerase eta vs. Ensembl Celegans
Match: polh-1 (DNA polymerase eta; POLH (DNA polymerase eta) homolog [Source:UniProtKB/TrEMBL;Acc:G5EFY1]) HSP 1 Score: 237.654 bits (605), Expect = 1.476e-69 Identity = 157/472 (33.26%), Postives = 246/472 (52.12%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTW--RGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVP--ENRGKADLTKYRNAGAEVIKCLSKHAS--CVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCH-AVGY-VSYSDWL--DHLMELRATDEQC-DDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLAD-GSWFPPLLSLGLSAGKFKPQMAAKN-DISRFLVDCPINKS 1447
+I LIDMDCFY QVE + P G+ VVQ++ GG I+AVSYEAR GV R M EAK KCP I + VP E KAD+ KYR+A AEV + L+ + S +E+AS+DEAF+D++ + L L + L + H A G V ++ L D L+E C +++ L + A V+Q+R+ ++ + CSAG+ ++K AK C HKP QQTL+P + +++ I +R GGKMG +Q+ LN++ MG+L + QL +TF ++ +L + G DE V R+ + SI SK FPG + ++ +++ WLD + +E+A+R++ D + N R ++ Y L + +K+L + P + + + + G+N D G W PP+L++ LSA +F+P + A+N I +L + KS
Sbjct: 4 VISLIDMDCFYAQVEQRDNPSLWGQPVIVVQHSRQGIEGG---------------ILAVSYEARPFGVKRGMTVAEAKLKCPQISICHVPIGEYVDKADIQKYRDASAEVFRVLNNYDSQIIIEKASVDEAFLDLSAYTNQKLQELRENEGLEEFLQAAITYLPTTHLATGEDVKENEHLREDVLLEYIENARNCTENLLLLIAAVTVEQIRQQIHEETQFFCSAGVGNNKMMAKLVCARHKPRQQTLIPWFYVREILRLTPIGDVRGFGGKMGNRIQEMLNITLMGELLEVDISQLIETFPNQHE-YLRSVAEGHCDEPVRPRKESSSIAVSKNFPGKLSIRSVLELKKWLDGLTKELAKRLATDQAENKRTAENLV--YSLLTEDGKPQKTLKITSYHP------DTLFEQIWAAMKGLNKTNATKNEDSGPWTPPILNISLSATRFQPGIPAQNRSIHEWLYEKKTRKS 451
BLAST of DNA polymerase eta vs. Ensembl Fly
Match: DNApol-eta (gene:FBgn0037141 transcript:FBtr0078489) HSP 1 Score: 305.449 bits (781), Expect = 3.947e-92 Identity = 171/475 (36.00%), Postives = 261/475 (54.95%), Query Frame = 2
Query: 59 KSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRAT----DEQCD--------------DVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLVD 1429
K + +++L+DMDCF+ QVE K P+Y + AVVQYN WRGGG IIAV+Y ARAKGV R MRGDEAK CP+I L VP R KAD +KYR+AG EV L + +ERAS+DEA++DIT+ V++ + + G A+ + + + AVGY S D+++ + A DE+ D+RL +GA + ++R V GY CSAGIAH+K AK A G++KPN+QT++P + L ++ + KI+ LGGK G V + L + MGQ+ FS L F +K G WL+++ RG D E+V R ++SIGC K+FPG N++ + +++WL +++ E+ +R+ +D N R HM V Y Q DG + ++ SR L++ I + + ++ ++ + + LG+S GKF+ + +N + +
Sbjct: 13 KYDRVVLLVDMDCFFCQVEEKQHPEYRNRPLAVVQYNPWRGGG--------------IIAVNYAARAKGVTRHMRGDEAKDLCPEIVLCQVPNIREKADTSKYRDAGKEVANVLQRFTQLLERASVDEAYLDITETVNHRMQQMQS-GAFAL----QPQELVNTFAVGYPSIGDYVNKITNRFANPYMDDERYQMSYDQNDLPAVRQSDIRLLIGASVAGEVRAAVKKETGYECSAGIAHNKILAKLAAGMNKPNKQTILPLTETASLFDSLPVGKIKGLGGKFGEVVCETLGIKFMGQVVKFSEVDLQRKFDEKNGTWLFNISRGIDLEAVTPRFYSKSIGCCKKFPGRNNITGLKTLQHWLGELSSEINDRLEKDFIENNRRAKHMVVQYVQDIDGEE----VASSRSTALRDYDQESIVRLSLDLIKANTKTFLRPGSESALNNAIKFLGISVGKFETVSSGQNKLQEMFAN 464
BLAST of DNA polymerase eta vs. Ensembl Fly
Match: DNApol-iota (gene:FBgn0037554 transcript:FBtr0081797) HSP 1 Score: 110.923 bits (276), Expect = 5.759e-25 Identity = 135/533 (25.33%), Postives = 216/533 (40.53%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNN-ASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRF-PGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAF--VVLSGVNTAGHCDLADGS----------WFPPLLS---------LGLSAGKFKPQMAAKNDISRFLV---DCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANEESNSSLDPPQQ 1594
I+ +DMD FY QVE P K + Q N ++ +Y ARAKGV + M EA++ CPD+ L + DL YR + L + VE+ DE F+D+T V+ + + A+L+ VG+ +D L D C RLA+G + Q++RE++ LG C AGIA++K AK H+PNQQT++ S M + ++ +G K + + +S++ QL + + FG +T L L G+D V ++IG P S + ++ R L ++ ++VAE + R+P + V + DS+K S +E A I + F + G LADG+ F ++ LGL+ KF+ + + I+ FL+ D + +S+ S +N S+ S T +S SD CA S ++ P Q
Sbjct: 17 IIHLDMDYFYAQVEEIRDPTLRSKALGIQQKNI-------------------VVTCNYVARAKGVTKLMLIAEAQRICPDLVLVN------GEDLAPYRQMSQRIFDLLLNYTPLVEKLGFDENFMDVTALVELRQAHVAE----ALLR----------PPVGHTYPADG----TPLSNCDCGCAQ-RLAIGTRIAQEIREELKLRLGITCCAGIAYNKLLAKLVGSSHEPNQQTVLVSTYAEQFMRELGDLKRVTGIGQKTQCLLLEA-GMSSVEQLQQCDMDVMRKKFGFETATRLRDLAFGRDTSLVRPSGKPKTIGMEDACKPISVRTDVEERFRMLLKRLVEQVAE--------DGRVPIAIKVVLRKF----DSQKKSSH------RETKQANILPSLFKTSMCPGETGVSKVQLADGAQDKLLKIVMRLFERIVDMSKPFNITLLGLAFSKFQERKVGSSSIANFLIKKADLEV-QSITSLTNTSL----------TSPTAESPTSD---ECAFRSSPTTFKPSDQ 472
BLAST of DNA polymerase eta vs. Ensembl Fly
Match: DNApol-iota (gene:FBgn0037554 transcript:FBtr0081798) HSP 1 Score: 110.923 bits (276), Expect = 5.759e-25 Identity = 135/533 (25.33%), Postives = 216/533 (40.53%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNN-ASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRF-PGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAF--VVLSGVNTAGHCDLADGS----------WFPPLLS---------LGLSAGKFKPQMAAKNDISRFLV---DCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANEESNSSLDPPQQ 1594
I+ +DMD FY QVE P K + Q N ++ +Y ARAKGV + M EA++ CPD+ L + DL YR + L + VE+ DE F+D+T V+ + + A+L+ VG+ +D L D C RLA+G + Q++RE++ LG C AGIA++K AK H+PNQQT++ S M + ++ +G K + + +S++ QL + + FG +T L L G+D V ++IG P S + ++ R L ++ ++VAE + R+P + V + DS+K S +E A I + F + G LADG+ F ++ LGL+ KF+ + + I+ FL+ D + +S+ S +N S+ S T +S SD CA S ++ P Q
Sbjct: 17 IIHLDMDYFYAQVEEIRDPTLRSKALGIQQKNI-------------------VVTCNYVARAKGVTKLMLIAEAQRICPDLVLVN------GEDLAPYRQMSQRIFDLLLNYTPLVEKLGFDENFMDVTALVELRQAHVAE----ALLR----------PPVGHTYPADG----TPLSNCDCGCAQ-RLAIGTRIAQEIREELKLRLGITCCAGIAYNKLLAKLVGSSHEPNQQTVLVSTYAEQFMRELGDLKRVTGIGQKTQCLLLEA-GMSSVEQLQQCDMDVMRKKFGFETATRLRDLAFGRDTSLVRPSGKPKTIGMEDACKPISVRTDVEERFRMLLKRLVEQVAE--------DGRVPIAIKVVLRKF----DSQKKSSH------RETKQANILPSLFKTSMCPGETGVSKVQLADGAQDKLLKIVMRLFERIVDMSKPFNITLLGLAFSKFQERKVGSSSIANFLIKKADLEV-QSITSLTNTSL----------TSPTAESPTSD---ECAFRSSPTTFKPSDQ 472
BLAST of DNA polymerase eta vs. Ensembl Fly
Match: DNApol-iota (gene:FBgn0037554 transcript:FBtr0339606) HSP 1 Score: 110.923 bits (276), Expect = 5.759e-25 Identity = 135/533 (25.33%), Postives = 216/533 (40.53%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNN-ASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRF-PGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAF--VVLSGVNTAGHCDLADGS----------WFPPLLS---------LGLSAGKFKPQMAAKNDISRFLV---DCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANEESNSSLDPPQQ 1594
I+ +DMD FY QVE P K + Q N ++ +Y ARAKGV + M EA++ CPD+ L + DL YR + L + VE+ DE F+D+T V+ + + A+L+ VG+ +D L D C RLA+G + Q++RE++ LG C AGIA++K AK H+PNQQT++ S M + ++ +G K + + +S++ QL + + FG +T L L G+D V ++IG P S + ++ R L ++ ++VAE + R+P + V + DS+K S +E A I + F + G LADG+ F ++ LGL+ KF+ + + I+ FL+ D + +S+ S +N S+ S T +S SD CA S ++ P Q
Sbjct: 17 IIHLDMDYFYAQVEEIRDPTLRSKALGIQQKNI-------------------VVTCNYVARAKGVTKLMLIAEAQRICPDLVLVN------GEDLAPYRQMSQRIFDLLLNYTPLVEKLGFDENFMDVTALVELRQAHVAE----ALLR----------PPVGHTYPADG----TPLSNCDCGCAQ-RLAIGTRIAQEIREELKLRLGITCCAGIAYNKLLAKLVGSSHEPNQQTVLVSTYAEQFMRELGDLKRVTGIGQKTQCLLLEA-GMSSVEQLQQCDMDVMRKKFGFETATRLRDLAFGRDTSLVRPSGKPKTIGMEDACKPISVRTDVEERFRMLLKRLVEQVAE--------DGRVPIAIKVVLRKF----DSQKKSSH------RETKQANILPSLFKTSMCPGETGVSKVQLADGAQDKLLKIVMRLFERIVDMSKPFNITLLGLAFSKFQERKVGSSSIANFLIKKADLEV-QSITSLTNTSL----------TSPTAESPTSD---ECAFRSSPTTFKPSDQ 472
BLAST of DNA polymerase eta vs. Ensembl Fly
Match: Rev1 (gene:FBgn0035150 transcript:FBtr0072557) HSP 1 Score: 86.2705 bits (212), Expect = 4.035e-17 Identity = 91/386 (23.58%), Postives = 154/386 (39.90%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAV-------------VQYNTWRGGGYTICELRFIQ-------------------SVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLV-PSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSE 1132
++ IDMDCF+V V L+ P+ G AV V R + RF S+ I + SYEAR KG+ M +A + CP+ L ++P D Y+ + ++++ +E S DE F++ LTDL ++ + + AF V +R++VY+ G CSAG+A +K A+ A KPN Q L+ S+ I M S+D + +G + ++Q ++ G + +L ++ G K G L+ CRG DD + Q+ +++ F G N+++ +L Q+++EV +R+ E
Sbjct: 275 VMHIDMDCFFVSVGLRTRPELRGLPIAVTHSKGGNAATDVPVHPQADRKAELELFAQRFEHHFHDGDKAEKVRSGFDKKMSLSEIASCSYEAREKGIRNGMFVGQALKLCPE--LKTIP-----YDFEGYKEVAFTLYDTVAQYTLNIEAVSCDEMFVE-----------LTDLAH-----------------------------------------ELNVDVMAF-VSHLRQEVYSKTGCPCSAGVAGNKLLARMATKEAKPNGQFLLDSSNDILAYMAPMSLDLLPGVGSSISHKLKQA-GLNNCGDVQNTTLEKMEKVLGKKLGQNLFQNCRGIDDRPLAYEQIRKTVSAEMNF-GIRFTNSVE-CEQFLCQLSEEVTKRLVE 597
BLAST of DNA polymerase eta vs. Ensembl Zebrafish
Match: polh (polymerase (DNA directed), eta [Source:ZFIN;Acc:ZDB-GENE-060421-4949]) HSP 1 Score: 344.739 bits (883), Expect = 3.962e-108 Identity = 191/453 (42.16%), Postives = 261/453 (57.62%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSD----------------WLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKF 1381
++ L+DMDCFYVQVE + P+ K V QY TW+GGG IIAVSYEARA GV R+M D+A++ CPD+ + VPE GKADLT YR A EVI+ +S+ A +ERASIDEA++D+T V L + + ++D + ++ H G+ + + WLD L +T+ D+RLA+GA +V+QMR V G+RCSAGI+H+K AK ACG++KPN+QT++P + L + I KIRNLGGK+G+ + + L+V MG L+ FS QL FGDKTGPWLY LCRG + E V RQL +SIGCSK F G L T +V+YWL Q+A E+ ER+++D N R+ + V Q ++S S SR L A ++ + ++ +NTAG A W P L L LSA KF
Sbjct: 8 VVALVDMDCFYVQVEQRLKPELKNKPCVVAQYKTWKGGG--------------IIAVSYEARAHGVGRNMFADDARKLCPDLQVARVPEAHGKADLTHYREASVEVIEVMSRFA-VIERASIDEAYMDLTASVQERLKNTS-------VQDITAQQLKNTHIQGFPTQQENTHLDRDAQRAAGLQQWLDLLSS--STESSPADLRLAVGALIVEQMRAAVEEHTGFRCSAGISHNKVLAKLACGLNKPNRQTVLPLSSVPQLFSTLPISKIRNLGGKLGSSITETLSVENMGDLTRFSRAQLEQHFGDKTGPWLYDLCRGIEFEPVKPRQLPKSIGCSKNFAGKTCLRTKQQVQYWLHQLALELEERLNKDRDVNGRVARQLTVGVRQA----GGQRSGSFSRCCALVRYDAMKMTNDSLTIIKSLNTAGAHQEA---WSPALTLLHLSASKF 429
BLAST of DNA polymerase eta vs. Ensembl Zebrafish
Match: polh (polymerase (DNA directed), eta [Source:ZFIN;Acc:ZDB-GENE-060421-4949]) HSP 1 Score: 344.739 bits (883), Expect = 5.150e-108 Identity = 191/453 (42.16%), Postives = 261/453 (57.62%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSD----------------WLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKF 1381
++ L+DMDCFYVQVE + P+ K V QY TW+GGG IIAVSYEARA GV R+M D+A++ CPD+ + VPE GKADLT YR A EVI+ +S+ A +ERASIDEA++D+T V L + + ++D + ++ H G+ + + WLD L +T+ D+RLA+GA +V+QMR V G+RCSAGI+H+K AK ACG++KPN+QT++P + L + I KIRNLGGK+G+ + + L+V MG L+ FS QL FGDKTGPWLY LCRG + E V RQL +SIGCSK F G L T +V+YWL Q+A E+ ER+++D N R+ + V Q ++S S SR L A ++ + ++ +NTAG A W P L L LSA KF
Sbjct: 8 VVALVDMDCFYVQVEQRLKPELKNKPCVVAQYKTWKGGG--------------IIAVSYEARAHGVGRNMFADDARKLCPDLQVARVPEAHGKADLTHYREASVEVIEVMSRFA-VIERASIDEAYMDLTASVQERLKNTS-------VQDITAQQLKNTHIQGFPTQQENTHLDRDAQRAAGLQQWLDLLSS--STESSPADLRLAVGALIVEQMRAAVEEHTGFRCSAGISHNKVLAKLACGLNKPNRQTVLPLSSVPQLFSTLPISKIRNLGGKLGSSITETLSVENMGDLTRFSRAQLEQHFGDKTGPWLYDLCRGIEFEPVKPRQLPKSIGCSKNFAGKTCLRTKQQVQYWLHQLALELEERLNKDRDVNGRVARQLTVGVRQA----GGQRSGSFSRCCALVRYDAMKMTNDSLTIIKSLNTAGAHQEA---WSPALTLLHLSASKF 429
BLAST of DNA polymerase eta vs. Ensembl Zebrafish
Match: polh (polymerase (DNA directed), eta [Source:ZFIN;Acc:ZDB-GENE-060421-4949]) HSP 1 Score: 344.739 bits (883), Expect = 5.150e-108 Identity = 191/453 (42.16%), Postives = 261/453 (57.62%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSD----------------WLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKF 1381
++ L+DMDCFYVQVE + P+ K V QY TW+GGG IIAVSYEARA GV R+M D+A++ CPD+ + VPE GKADLT YR A EVI+ +S+ A +ERASIDEA++D+T V L + + ++D + ++ H G+ + + WLD L +T+ D+RLA+GA +V+QMR V G+RCSAGI+H+K AK ACG++KPN+QT++P + L + I KIRNLGGK+G+ + + L+V MG L+ FS QL FGDKTGPWLY LCRG + E V RQL +SIGCSK F G L T +V+YWL Q+A E+ ER+++D N R+ + V Q ++S S SR L A ++ + ++ +NTAG A W P L L LSA KF
Sbjct: 8 VVALVDMDCFYVQVEQRLKPELKNKPCVVAQYKTWKGGG--------------IIAVSYEARAHGVGRNMFADDARKLCPDLQVARVPEAHGKADLTHYREASVEVIEVMSRFA-VIERASIDEAYMDLTASVQERLKNTS-------VQDITAQQLKNTHIQGFPTQQENTHLDRDAQRAAGLQQWLDLLSS--STESSPADLRLAVGALIVEQMRAAVEEHTGFRCSAGISHNKVLAKLACGLNKPNRQTVLPLSSVPQLFSTLPISKIRNLGGKLGSSITETLSVENMGDLTRFSRAQLEQHFGDKTGPWLYDLCRGIEFEPVKPRQLPKSIGCSKNFAGKTCLRTKQQVQYWLHQLALELEERLNKDRDVNGRVARQLTVGVRQA----GGQRSGSFSRCCALVRYDAMKMTNDSLTIIKSLNTAGAHQEA---WSPALTLLHLSASKF 429
BLAST of DNA polymerase eta vs. Ensembl Zebrafish
Match: poli (polymerase (DNA directed) iota [Source:ZFIN;Acc:ZDB-GENE-050417-376]) HSP 1 Score: 117.857 bits (294), Expect = 4.469e-27 Identity = 99/367 (26.98%), Postives = 169/367 (46.05%), Query Frame = 2
Query: 44 DH*KMKSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRR--DRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNAS-IDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSED 1135
D + S +I+ D+DCFY QVE+ P K + Q ++I I+ +Y AR GVN+ M +A +KCP + L +G+ DLT YR +V + L + VER DE F+D+T+ V+ +K+ R D S G H + S DE+ ++RLA+G+ + +MR+ ++++LG AG+A +K AK G KPNQQT + ++LM++ + + K+ +G K + + L + + L + L L FG+ + +L G DD V QS+ F ++T+++V ++++ + ER+ +D
Sbjct: 29 DRVSLTSQRVILHFDLDCFYAQVEMIRNPALRTKPLGIQQ--------------KYI-----IVTSNYVARELGVNKLMSVTDAVEKCPQLVLV-----KGE-DLTHYREMSYKVTELLMSYCPLVERLGFDENFMDVTEMVEK------------RIKETRISDLSFNG-HVYNHESV-----------VVDEE--NIRLAVGSVIAAEMRQAIFSTLGLTGCAGVASNKLLAKLVSGSFKPNQQTTLLPHSTAELMSSLTGLIKVPGIGYKTREKL-KALGLVNVRDLQLYPLSDLVKEFGEMNAKRIQNLACGIDDSPVTPAGPPQSLSDEDSF---KKMSTLEEVLKKIEELLINLTERMHKD 340
BLAST of DNA polymerase eta vs. Ensembl Zebrafish
Match: rev1 (REV1 DNA directed polymerase [Source:ZFIN;Acc:ZDB-GENE-081105-180]) HSP 1 Score: 90.5077 bits (223), Expect = 3.376e-18 Identity = 97/394 (24.62%), Postives = 148/394 (37.56%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAVVQYN-----TWRGGGYTICELRFIQ--------------------------------------SVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERV 1126
++ +DMDCF+V V ++ P +GK AV R G E ++ Q S+ I + SYEAR GV M AKQ CPD+ SVP D Y+ + + L+ + +E S DEA +D T LLV EL + D RSI RED+ G S G++ + A+ A KP Q L+ S+ + D + + + + +G M + + L VST G L SL QL FG +TG L+ CRG DD V S + +S+ + + +++ +L+ ++ EV +R+
Sbjct: 452 VLHVDMDCFFVSVGIRHRPDLIGKPVAVTSNRGPGRVAQRPGANPQLEFQYYQNKQKHYRKEKTDGDLEMTPSPQDGEVPSNGVHEDLAALSMAEIASCSYEARQAGVRNGMFFGRAKQLCPDLQ--SVP-----YDFHAYKEVALAMYEILASYTHNIEALSCDEALVDAT----ALLV------ELGVSPDELARSI-------------------------------------------REDIKEKTGCCASVGMSSNILLARMATRKAKPKGQYLLRSEEVDDFIRDQPVSSLPGVGRSMSSKLTS-LGVSTCGDLQQLSLSQLQREFGPRTGQTLFRFCRGLDDRPVRSEKERKSVSAEMNY--NIRFTQVEEAESFLNNLSMEVQKRL 782
BLAST of DNA polymerase eta vs. Ensembl Xenopus
Match: ngef (neuronal guanine nucleotide exchange factor [Source:Xenbase;Acc:XB-GENE-950834]) HSP 1 Score: 132.494 bits (332), Expect = 1.668e-31 Identity = 110/380 (28.95%), Postives = 172/380 (45.26%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLN---VSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGN 1201
+IV IDMDCFY QVE+ P+ K + Q ++I ++ +YEAR GV + M +A++KCP + L S DLT YR + L + + VER DE +ID+T+ VD L G R+ C G+V YSD + VR+A G+ + ++R +YN LG AG A +K AK G HKPNQQT + + S L+N S+D ++ + G +G ++L +S + L A + L FG + L RG+DD +V+ QSI F +T+ +V+ +++ + + R+S+D R+P + ++ Q N
Sbjct: 51 VIVHIDMDCFYAQVEMIRNPELRNKPLGIQQ--------------KYI-----VVTCNYEARKFGVTKLMLIKDAREKCPQLVLVS------GEDLTPYREMSYRATELLEEFSPQVERLGFDENYIDVTELVDKKLQEERGNG----------RNPGVC---GHV-YSDQ-------KMNVNNWAHVRIAAGSHIASEIRAALYNRLGLTGCAGTASNKLLAKLVSGTHKPNQQTALLHESHSHLIN--SLDHVKQIPG-IGYKTSKRLESLGLSRISDLQACPITLLEKEFGSSVAHRIQMLSRGEDDSAVVPSGPPQSISDEDSF---KKCSTVSEVKIKMEERLRNLLVRISKD----GRIPHTLRLTIRQFSPSN 374
BLAST of DNA polymerase eta vs. Ensembl Xenopus
Match: ngef (neuronal guanine nucleotide exchange factor [Source:Xenbase;Acc:XB-GENE-950834]) HSP 1 Score: 115.161 bits (287), Expect = 6.011e-26 Identity = 96/333 (28.83%), Postives = 152/333 (45.65%), Query Frame = 2
Query: 212 IQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLN---VSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGN 1201
IQ ++ +YEAR GV + M +A++KCP + L S DLT YR + L + + VER DE +ID+T+ VD L G R+ C G+V YSD + VR+A G+ + ++R +YN LG AG A +K AK G HKPNQQT + + S L+N S+D ++ + G +G ++L +S + L A + L FG + L RG+DD +V+ QSI F +T+ +V+ +++ + + R+S+D R+P + ++ Q N
Sbjct: 54 IQQKYIVVTCNYEARKFGVTKLMLIKDAREKCPQLVLVS------GEDLTPYREMSYRATELLEEFSPQVERLGFDENYIDVTELVDKKLQEERGNG----------RNPGVC---GHV-YSDQ-------KMNVNNWAHVRIAAGSHIASEIRAALYNRLGLTGCAGTASNKLLAKLVSGTHKPNQQTALLHESHSHLIN--SLDHVKQIPG-IGYKTSKRLESLGLSRISDLQACPITLLEKEFGSSVAHRIQMLSRGEDDSAVVPSGPPQSISDEDSF---KKCSTVSEVKIKMEERLRNLLVRISKD----GRIPHTLRLTIRQFSPSN 349
BLAST of DNA polymerase eta vs. Ensembl Xenopus
Match: ngef (neuronal guanine nucleotide exchange factor [Source:Xenbase;Acc:XB-GENE-950834]) HSP 1 Score: 87.0409 bits (214), Expect = 3.554e-17 Identity = 72/263 (27.38%), Postives = 126/263 (47.91%), Query Frame = 2
Query: 434 IDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHL-MELRATDEQCDD---VRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLN---VSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGN 1201
I + +I +T + +T +L ++KD R++ C + VS D + M RAT+ ++ VR+A G+ + ++R +YN LG AG A +K AK G HKPNQQT + + S L+N S+D ++ + G +G ++L +S + L A + L FG + L RG+DD +V+ QSI F +T+ +V+ +++ + + R+S+D R+P + ++ Q N
Sbjct: 79 IQQKYIVVTCNYEARKFGVT---KLMLIKDAREK----CPQLVLVSGEDLTPYREMSYRATEMNVNNWAHVRIAAGSHIASEIRAALYNRLGLTGCAGTASNKLLAKLVSGTHKPNQQTALLHESHSHLIN--SLDHVKQIPG-IGYKTSKRLESLGLSRISDLQACPITLLEKEFGSSVAHRIQMLSRGEDDSAVVPSGPPQSISDEDSF---KKCSTVSEVKIKMEERLRNLLVRISKD----GRIPHTLRLTIRQFSPSN 324 HSP 2 Score: 54.299 bits (129), Expect = 6.645e-7 Identity = 34/100 (34.00%), Postives = 48/100 (48.00%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYR 370
+IV IDMDCFY QVE+ P+ K + Q ++I ++ +YEAR GV + M +A++KCP + L S DLT YR
Sbjct: 51 VIVHIDMDCFYAQVEMIRNPELRNKPLGIQQ--------------KYI-----VVTCNYEARKFGVTKLMLIKDAREKCPQLVLVS------GEDLTPYR 125
BLAST of DNA polymerase eta vs. Ensembl Xenopus
Match: trmo (tRNA methyltransferase O [Source:Xenbase;Acc:XB-GENE-1004391]) HSP 1 Score: 85.5001 bits (210), Expect = 1.514e-16 Identity = 85/387 (21.96%), Postives = 137/387 (35.40%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAVVQ-----YNTWRGGGYTICELRFIQ-------------------------------SVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERV 1126
I+ +DMDCF+V V ++ P GK AV T R G E ++ Q S+ I + SYEAR GV M +AKQ CP++ +VP D Y+ + + L+ + +E S DEA DIT L E R T ++ + +R +V G S GI + A+ A KP+ Q + + + D + ++ + +G + + L V T G L ++ +L FG KTG LY CRG DD + + +S+ + + +L +++E+ R+
Sbjct: 414 IIHVDMDCFFVSVAIRNRPDLKGKPVAVTSNRGAGTTTTREGANPQLEFQYYQNRILKGKADQPTSISSDSAQQNGLDLDISHLSMAEIASCSYEARQAGVKNGMFFGKAKQLCPELQ--AVP-----YDFDAYKEVAMTMYRILASYTHDIEAVSCDEALADITG------------------------------------------ILTETRLTPDEASNA-----------IRTEVKEKTGCAASVGIGSNILLARMATRRAKPDGQYHLKPEEVDDFIRGQLVNNLPGVGRTI-EYKLSCLGVKTCGDLQNITMAKLQKEFGPKTGQMLYRFCRGLDDRPIRKEKERKSVSAEINY--GIRFTQTSEAEAFLINLSEEIQRRL 737
BLAST of DNA polymerase eta vs. Ensembl Xenopus
Match: trmo (tRNA methyltransferase O [Source:Xenbase;Acc:XB-GENE-1004391]) HSP 1 Score: 85.5001 bits (210), Expect = 1.805e-16 Identity = 85/387 (21.96%), Postives = 137/387 (35.40%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAVVQ-----YNTWRGGGYTICELRFIQ-------------------------------SVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERV 1126
I+ +DMDCF+V V ++ P GK AV T R G E ++ Q S+ I + SYEAR GV M +AKQ CP++ +VP D Y+ + + L+ + +E S DEA DIT L E R T ++ + +R +V G S GI + A+ A KP+ Q + + + D + ++ + +G + + L V T G L ++ +L FG KTG LY CRG DD + + +S+ + + +L +++E+ R+
Sbjct: 412 IIHVDMDCFFVSVAIRNRPDLKGKPVAVTSNRGAGTTTTREGANPQLEFQYYQNRILKGKADQPTSISSDSAQQNGLDLDISHLSMAEIASCSYEARQAGVKNGMFFGKAKQLCPELQ--AVP-----YDFDAYKEVAMTMYRILASYTHDIEAVSCDEALADITG------------------------------------------ILTETRLTPDEASNA-----------IRTEVKEKTGCAASVGIGSNILLARMATRRAKPDGQYHLKPEEVDDFIRGQLVNNLPGVGRTI-EYKLSCLGVKTCGDLQNITMAKLQKEFGPKTGQMLYRFCRGLDDRPIRKEKERKSVSAEINY--GIRFTQTSEAEAFLINLSEEIQRRL 735
BLAST of DNA polymerase eta vs. Ensembl Mouse
Match: Polh (polymerase (DNA directed), eta (RAD 30 related) [Source:MGI Symbol;Acc:MGI:1891457]) HSP 1 Score: 317.39 bits (812), Expect = 4.013e-98 Identity = 202/486 (41.56%), Postives = 275/486 (56.58%), Query Frame = 2
Query: 65 NPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSL------TDLGELAMLKD-RRDRSIEGC---HAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQM-AAKNDISRFLVDCPINKSVASTSNKSVPIADS 1489
N ++ L+DMDCF+VQVE + P K AVVQY +W+GGG IIAVSYEARA GV R+M D+AK+ CPD+ L V E+RGKA+LTKYR A EV++ +S A +ERASIDEA+ID+T V L L DL ++ R ++E A+ WLD L++ D D+RL +GA +V++MR + + G++CSAGI+H+K AK ACG++KPN+QTLV + L + I KIR+LGGK+G V + L + MG L+ F+ QL FG+K G WLY +CRG + + V RQL ++IGCSK FPG L T ++V++WL Q+A E+ ER+++D + N R+ T + VS +R D R S S R L A +S+ AF + NT+G W PPL L L A KF A DI+ FL S S+ VPIA S
Sbjct: 6 NRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGG--------------IIAVSYEARAFGVTRNMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVMEIMSYFA-VIERASIDEAYIDLTSAVQERLQKLQGQPISADLLPSTYIEGLPRGPTVEETVQKEAIRKQGLLQWLD---SLQSDDPTSPDLRLTVGAMIVEEMRAAIESKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEVLGIEYMGDLTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLALELEERLTKDRNDNDRVATQLVVS---IRFQGDRRLS-SLRRCCALPRYDAHKMSQDAFAAIRNCNTSG----IQTEWSPPLTMLFLCATKFSAAAPPACTDITAFL-------SSDSSCQPKVPIASS 458
BLAST of DNA polymerase eta vs. Ensembl Mouse
Match: Poli (polymerase (DNA directed), iota [Source:MGI Symbol;Acc:MGI:1347081]) HSP 1 Score: 115.931 bits (289), Expect = 2.374e-26 Identity = 89/332 (26.81%), Postives = 151/332 (45.48%), Query Frame = 2
Query: 53 KMKSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNAS-IDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRF 1045
++ S+ +IV +D+DCFY QVE+ + P+ + V Q +++ ++ +YEAR GV + M +AK+KCP + L + DL++YR +V + L + + VER DE F+D+T+ V+ L L + G+V Y++ +L + RL +G+ + +MRE +YN LG AG+A +K AK GV KPNQQT++ + L+++ + I +I +G K + + L ++++ L F + L G + L G+D V QS F
Sbjct: 43 QISSSRVIVHVDLDCFYAQVEMISNPELKDRPLGVQQ--------------KYL-----VVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVN------GEDLSRYREMSYKVTELLEEFSPAVERLGFDENFVDLTEMVEKRLQQLPS------------EEVPSVTVFGHV-YNNQSVNLHNIMHR-------RLVVGSQIAAEMREAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKRL-EVLGINSVHDLQTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTF 328
BLAST of DNA polymerase eta vs. Ensembl Mouse
Match: Polh (polymerase (DNA directed), eta (RAD 30 related) [Source:MGI Symbol;Acc:MGI:1891457]) HSP 1 Score: 102.449 bits (254), Expect = 1.482e-25 Identity = 52/100 (52.00%), Postives = 65/100 (65.00%), Query Frame = 2
Query: 65 NPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTK 364
N ++ L+DMDCF+VQVE + P K AVVQY +W+GGG IIAVSYEARA GV R+M D+AK+ CPD+ L V E+RGKA+LTK
Sbjct: 6 NRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGG--------------IIAVSYEARAFGVTRNMWADDAKKLCPDLLLAQVRESRGKANLTK 91
BLAST of DNA polymerase eta vs. Ensembl Mouse
Match: Poli (polymerase (DNA directed), iota [Source:MGI Symbol;Acc:MGI:1347081]) HSP 1 Score: 100.523 bits (249), Expect = 1.661e-21 Identity = 76/279 (27.24%), Postives = 126/279 (45.16%), Query Frame = 2
Query: 212 IQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNAS-IDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRF 1045
+Q ++ +YEAR GV + M +AK+KCP + L + DL++YR +V + L + + VER DE F+D+T+ V+ L L + G+V Y++ +L + RL +G+ + +MRE +YN LG AG+A +K AK GV KPNQQT++ + L+++ + I +I +G K + + L ++++ L F + L G + L G+D V QS F
Sbjct: 14 VQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVN------GEDLSRYREMSYKVTELLEEFSPAVERLGFDENFVDLTEMVEKRLQQLPS------------EEVPSVTVFGHV-YNNQSVNLHNIMHR-------RLVVGSQIAAEMREAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKRL-EVLGINSVHDLQTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTF 265
BLAST of DNA polymerase eta vs. Ensembl Mouse
Match: Poli (polymerase (DNA directed), iota [Source:MGI Symbol;Acc:MGI:1347081]) HSP 1 Score: 100.523 bits (249), Expect = 1.661e-21 Identity = 76/279 (27.24%), Postives = 126/279 (45.16%), Query Frame = 2
Query: 212 IQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNAS-IDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRF 1045
+Q ++ +YEAR GV + M +AK+KCP + L + DL++YR +V + L + + VER DE F+D+T+ V+ L L + G+V Y++ +L + RL +G+ + +MRE +YN LG AG+A +K AK GV KPNQQT++ + L+++ + I +I +G K + + L ++++ L F + L G + L G+D V QS F
Sbjct: 14 VQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVN------GEDLSRYREMSYKVTELLEEFSPAVERLGFDENFVDLTEMVEKRLQQLPS------------EEVPSVTVFGHV-YNNQSVNLHNIMHR-------RLVVGSQIAAEMREAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKRL-EVLGINSVHDLQTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTF 265
BLAST of DNA polymerase eta vs. UniProt/SwissProt
Match: sp|Q9Y253|POLH_HUMAN (DNA polymerase eta OS=Homo sapiens OX=9606 GN=POLH PE=1 SV=1) HSP 1 Score: 331.643 bits (849), Expect = 1.608e-102 Identity = 200/495 (40.40%), Postives = 275/495 (55.56%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSL------------TDLGELAMLKDRRDRSI--EGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQM-AAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNS 1510
++ L+DMDCF+VQVE + P K AVVQY +W+GGG IIAVSYEARA GV RSM D+AK+ CPD+ L V E+RGKA+LTKYR A EV++ +S+ A +ERASIDEA++D+T V L L T + L + ++ EG G WLD L T D++L +GA +V++MR + G++CSAGI+H+K AK ACG++KPN+QTLV + L + I KIR+LGGK+G V + L + MG+L+ F+ QL FG+K G WLY +CRG + + V RQL ++IGCSK FPG L T ++V++WL Q+AQE+ ER+++D + N R+ T + VS +R D R S S R L A +S AF V+ NT+G W PPL L L A KF ++ DI+ FL P +S VP+ S + S
Sbjct: 8 VVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGG--------------IIAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVMEIMSRFA-VIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYIEGLPQGPTTAEETVQKEGMRKQGLF---QWLDSLQIDNLTS---PDLQLTVGAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERLTKDRNDNDRVATQLVVS---IRVQGDKRLS-SLRRCCALTRYDAHKMSHDAFTVIKNCNTSG----IQTEWSPPLTMLFLCATKFSASAPSSSTDITSFLSSDP-------SSLPKVPVTSSEAKTQGS 466
BLAST of DNA polymerase eta vs. UniProt/SwissProt
Match: sp|Q9JJN0|POLH_MOUSE (DNA polymerase eta OS=Mus musculus OX=10090 GN=Polh PE=1 SV=1) HSP 1 Score: 317.39 bits (812), Expect = 2.807e-97 Identity = 202/486 (41.56%), Postives = 275/486 (56.58%), Query Frame = 2
Query: 65 NPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSL------TDLGELAMLKD-RRDRSIEGC---HAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQM-AAKNDISRFLVDCPINKSVASTSNKSVPIADS 1489
N ++ L+DMDCF+VQVE + P K AVVQY +W+GGG IIAVSYEARA GV R+M D+AK+ CPD+ L V E+RGKA+LTKYR A EV++ +S A +ERASIDEA+ID+T V L L DL ++ R ++E A+ WLD L++ D D+RL +GA +V++MR + + G++CSAGI+H+K AK ACG++KPN+QTLV + L + I KIR+LGGK+G V + L + MG L+ F+ QL FG+K G WLY +CRG + + V RQL ++IGCSK FPG L T ++V++WL Q+A E+ ER+++D + N R+ T + VS +R D R S S R L A +S+ AF + NT+G W PPL L L A KF A DI+ FL S S+ VPIA S
Sbjct: 6 NRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGG--------------IIAVSYEARAFGVTRNMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVMEIMSYFA-VIERASIDEAYIDLTSAVQERLQKLQGQPISADLLPSTYIEGLPRGPTVEETVQKEAIRKQGLLQWLD---SLQSDDPTSPDLRLTVGAMIVEEMRAAIESKTGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEVLGIEYMGDLTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLALELEERLTKDRNDNDRVATQLVVS---IRFQGDRRLS-SLRRCCALPRYDAHKMSQDAFAAIRNCNTSG----IQTEWSPPLTMLFLCATKFSAAAPPACTDITAFL-------SSDSSCQPKVPIASS 458
BLAST of DNA polymerase eta vs. UniProt/SwissProt
Match: sp|Q9VNX1|POLH_DROME (DNApol-eta OS=Drosophila melanogaster OX=7227 GN=DNApol-eta PE=1 SV=1) HSP 1 Score: 305.449 bits (781), Expect = 3.956e-91 Identity = 171/475 (36.00%), Postives = 261/475 (54.95%), Query Frame = 2
Query: 59 KSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRAT----DEQCD--------------DVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLVD 1429
K + +++L+DMDCF+ QVE K P+Y + AVVQYN WRGGG IIAV+Y ARAKGV R MRGDEAK CP+I L VP R KAD +KYR+AG EV L + +ERAS+DEA++DIT+ V++ + + G A+ + + + AVGY S D+++ + A DE+ D+RL +GA + ++R V GY CSAGIAH+K AK A G++KPN+QT++P + L ++ + KI+ LGGK G V + L + MGQ+ FS L F +K G WL+++ RG D E+V R ++SIGC K+FPG N++ + +++WL +++ E+ +R+ +D N R HM V Y Q DG + ++ SR L++ I + + ++ ++ + + LG+S GKF+ + +N + +
Sbjct: 13 KYDRVVLLVDMDCFFCQVEEKQHPEYRNRPLAVVQYNPWRGGG--------------IIAVNYAARAKGVTRHMRGDEAKDLCPEIVLCQVPNIREKADTSKYRDAGKEVANVLQRFTQLLERASVDEAYLDITETVNHRMQQMQS-GAFAL----QPQELVNTFAVGYPSIGDYVNKITNRFANPYMDDERYQMSYDQNDLPAVRQSDIRLLIGASVAGEVRAAVKKETGYECSAGIAHNKILAKLAAGMNKPNKQTILPLTETASLFDSLPVGKIKGLGGKFGEVVCETLGIKFMGQVVKFSEVDLQRKFDEKNGTWLFNISRGIDLEAVTPRFYSKSIGCCKKFPGRNNITGLKTLQHWLGELSSEINDRLEKDFIENNRRAKHMVVQYVQDIDGEE----VASSRSTALRDYDQESIVRLSLDLIKANTKTFLRPGSESALNNAIKFLGISVGKFETVSSGQNKLQEMFAN 464
BLAST of DNA polymerase eta vs. UniProt/SwissProt
Match: sp|Q8H2D5|POLH_ARATH (DNA polymerase eta OS=Arabidopsis thaliana OX=3702 GN=POLH PE=1 SV=1) HSP 1 Score: 283.493 bits (724), Expect = 8.837e-85 Identity = 160/397 (40.30%), Postives = 229/397 (57.68%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELR----ATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGN-DSRKSLSGSRVVPLK 1246
+I +DMDCFYVQVE + P+ G SAVVQYN W+GGG +IAVSYEAR GV RSMRGDEAK CP I L VP RGKADL YR+AG+EV+ L+K C ERASIDE ++D+TD +++L L ++ D + H +G ++ D D +R D D L+ G +V ++R+ V + CSAGIAH+K AK A G++KP QQT+VP + +L+++ I K++ LGGK+GT +Q L V T+G L FS +L + +G TG WL+++ RG E V R L +S G K FPG L ++ V++WL+Q+++E++ER+ D N R+ + + + R + DS K S+ P++
Sbjct: 13 VIAHVDMDCFYVQVEQRKQPELRGLPSAVVQYNEWQGGG--------------LIAVSYEARKCGVKRSMRGDEAKAACPQIQLVQVPVARGKADLNLYRSAGSEVVSILAKSGKC-ERASIDEVYLDLTDAAESMLADAPPES-LELI----DEEVLKSHILG-MNREDGDDFKESVRNWICREDADRRDKLLSCGIIIVAELRKQVLKETEFTCSAGIAHNKMLAKLASGMNKPAQQTVVPYAAVQELLSSLPIKKMKQLGGKLGTSLQTDLGVDTVGDLLQFSETKLQEHYGVNTGTWLWNIARGISGEEVQGRLLPKSHGSGKTFPGPRALKSLSTVQHWLNQLSEELSERLGSDLEQNKRIASTLTLHASAFRSKDSDSHKKFP-SKSCPMR 387
BLAST of DNA polymerase eta vs. UniProt/SwissProt
Match: sp|O42917|ESO1_SCHPO (N-acetyltransferase eso1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eso1 PE=1 SV=1) HSP 1 Score: 139.043 bits (349), Expect = 9.214e-33 Identity = 114/411 (27.74%), Postives = 188/411 (45.74%), Query Frame = 2
Query: 32 LRFWDH*KMKSNPIIVL--IDMDCFYVQVELKAAPQYVGKESAV-VQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDI-----------------HLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLK-DRRDRSIEGCHA--------VGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVS 1177
L++ D +++P+ V+ ID D FY QVE +G + +V + W+G +IAV+Y ARA ++R EAK+KCP++ H P N K L YR+ +++ + KHA V++ASIDE FI++T +V ++ L E LK D ++ A G V + +D E D DDV L A +V+++R+D+Y L Y CSAG++ + +K +KPN+QT++ + I D + + I IR LGGK G + L ++ + S+ L D G GP +++LC G D+ + ++ +S+ +K F + D + W A ++ R E R P +C++
Sbjct: 13 LQYCDKAGTQNSPLRVVAHIDQDAFYAQVE----SVRLGLDHSVPLAVQQWQG----------------LIAVNYAARAANISRHETVTEAKKKCPELCTAHVKTWKAGESEAKYHENPNP-NYYKTCLDPYRHESVKILNIIKKHAPVVKKASIDECFIELTSDVKRIV-----LEEYPYLKIPSEDSNVALPQAPVLLWPAEFGMVIEEEVVDRTKEDYERD--WDDVFLFYAAKIVKEIRDDIYLQLKYTCSAGVSFNPMLSKLVSSRNKPNKQTILTKNAIQDYLVSLKITDIRMLGGKFGEEIINLLGTDSIKDVWNMSMDFLIDKLGQTNGPLVWNLCHGIDNTEITTQVQIKSMLSAKNFSQQKVKSEEDAIN-WFQVFASDLRSRFLE--LEGMRRPKTICLT 392
BLAST of DNA polymerase eta vs. TrEMBL
Match: A0A1S3KD68 (DNA polymerase eta OS=Lingula unguis OX=7574 GN=LOC106180842 PE=4 SV=1) HSP 1 Score: 382.874 bits (982), Expect = 9.800e-120 Identity = 211/469 (44.99%), Postives = 277/469 (59.06%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGY----------------VSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMAAKN-DISRFLV 1426
+I L+DMDCFYVQVE + P GK AVVQYN W+GGG IIAV YEARA GV R MRGDEAK+KCPDI L VPE RGKADLTK+R AGAEVI LSK CVERASIDEA++D+T EV+ L ++G +L + ++ + VG+ DWL + A +Q DVRLA+GA + ++MR VY G+RCSAGIAH+K AKF+CGVHKPN+QT++P + + + K+R GGK G V Q+L V + + +S QL + FG+K G WL+ +CRG++ E+V +RQL +SIGCSK F G N L+T +KVRYWLDQ++ E+ ER+ +D N R+ H+ VS L + N +S SR + + I AF +L NTAG +W P ++ LG+SAGKF N I +FLV
Sbjct: 4 VIALVDMDCFYVQVEQRLNPALKGKPCAVVQYNAWKGGG--------------IIAVGYEARACGVTRQMRGDEAKKKCPDIVLCRVPEVRGKADLTKFREAGAEVIATLSKFCKCVERASIDEAYLDLTAEVEE---RLNEIGNTRILASQ----LQNTYVVGWEGEDSQKTQDGLDLREQGVKDWLSTCYD--AVSDQ--DVRLAVGAVIAEEMRAAVYKETGFRCSAGIAHNKMLAKFSCGVHKPNKQTVLPHSSVHKMFTTLKVTKLRGFGGKFGEEVIQKLGVENVADICKYSEQQLQNYFGEKAGSWLFAVCRGEEHEAVSARQLAKSIGCSKNFTGKNCLDTREKVRYWLDQLSGELEERLIKDKEVNNRVAKHLNVSVRYLSNPN----PVSSSRSCAIVKYERKKICDDAFSLLQKFNTAGP---HQSAWVPAIICLGMSAGKFDEMSNTANPTIKQFLV 440
BLAST of DNA polymerase eta vs. TrEMBL
Match: A0A2R5LBP5 (Putative dna polymerase iota/dna damage inducible protein (Fragment) OS=Ornithodoros turicata OX=34597 PE=4 SV=1) HSP 1 Score: 381.333 bits (978), Expect = 5.169e-119 Identity = 222/507 (43.79%), Postives = 295/507 (58.19%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEV--DNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMA-AKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANEESNSSLDP 1585
IVL+DMDCFYVQVE K APQY GK AV QYN ++GGG +IAV+YEARA G+ R MRGD A + C D+HLF VPE RGKADLT+YRNAG EV++ +S+ + +ERASIDEA++D++D + D +V+ TDL ++ +G V S+WL + A D D LA A LV+++R V GYRCSAGIAH+K AK ACG+HKPNQQT++ + L ++K+RNLGGK+G +++ L V T+G+++ FS L TFG+KTG WLYHL RG D E V R+L +SIGC K F G LNT DKV+YW+ Q+A+E+AER+ D N+R + V Q G + SR PL + I A VLS +NTA ADGSWFPPL ++ L+A KFK + DIS+FL P + S+ ++P S DS S +LR +S ++ P
Sbjct: 8 IVLVDMDCFYVQVEEKLAPQYKGKPCAVSQYNQFQGGG--------------LIAVNYEARAAGIKRGMRGDAAMKLCRDLHLFRVPEVRGKADLTRYRNAGGEVLQVMSQFSGVIERASIDEAYLDLSDVLRDDAEIVAPTDLPNTHVVGSSGTADSDG--DVRAEGVSNWLSSI----AVDSP--DYLLARAAKLVEEIRLAVLRETGYRCSAGIAHNKVLAKLACGLHKPNQQTVLTQGAVPSLFATLPVNKVRNLGGKLGEDIKETLQVETLGEVTRFSQESLITTFGEKTGSWLYHLSRGIDHEPVTLRKLAKSIGCGKNFNGKLALNTRDKVKYWVQQLAEELAERLQRDREQNSRTAQLLVVGVRQNHPGT------AVSRSCPLTCYDSGRIMNDALSVLSKLNTASP---ADGSWFPPLCNISLAASKFKDTIKEGTQDISKFLSAGPSATGMELKSSSTLPETLS----------DSHPSKDTLRDTASKSEETIKP 473
BLAST of DNA polymerase eta vs. TrEMBL
Match: A0A2T7NW77 (UmuC domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_13067 PE=4 SV=1) HSP 1 Score: 374.785 bits (961), Expect = 1.043e-115 Identity = 213/474 (44.94%), Postives = 275/474 (58.02%), Query Frame = 2
Query: 65 NPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAM--------------LKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDK-VRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKF-KPQMAAKNDISRFLVDCPI 1438
N +VL+DMDCFYVQVE + P GK AVVQY T++GGG IIAV YEARA GV RSM GD+AK+ CPDI L VPE RGKADLTKYR AGAEVI+ LS +CVERASIDEA++D+T+E LL S T G L +++ DR+ A + + WL + T+ DV LA A +V++MR ++ + G++CSAGIAH+K AK ACG+HKPN+QT+VP + + + KIR+LGGK+G + ++L V MG L FSL QL +G KTG WL+ CRG D ESV +RQL +SIGCSKRF G L+ +K V YW+ Q+A+EV+ER+ +D N R MCVSY S++GSR L ++ AAF +L NT G C SW P + LGLSA KF + Q A DI+ +
Sbjct: 2 NRAVVLVDMDCFYVQVEQRLNPDLKGKPCAVVQYKTYKGGG--------------IIAVGYEARALGVTRSMMGDDAKKICPDIVLARVPEVRGKADLTKYREAGAEVIEVLSNFCNCVERASIDEAYLDLTEEAKKLLTSQTS-GSLTADQLSNTFVEGFGDDVEEDMDRAQLNEEAARHRNTDKWLKQAHASKETNS--GDVLLAATALIVEEMRATIFKNTGFQCSAGIAHNKMLAKLACGLHKPNKQTIVPQGSVEKIFMTLPVRKIRSLGGKLGASLTEELQVQNMGDLCQFSLQQLQTCYGSKTGLWLHQACRGFDYESVSARQLPKSIGCSKRFLGKERLDKKEKVVLYWIGQLAKEVSERLIKDQEINKRTAKSMCVSYQYCAKPT----SVTGSRACALVSYDHKKMTDAAFGLLQKFNTCGAC---KDSWSPSITVLGLSATKFEEEQSATHQDIATLFASAAV 451
BLAST of DNA polymerase eta vs. TrEMBL
Match: A0A1Z5LFU5 (DNA polymerase eta (Fragment) OS=Ornithodoros moubata OX=6938 PE=4 SV=1) HSP 1 Score: 374.015 bits (959), Expect = 1.118e-115 Identity = 205/469 (43.71%), Postives = 287/469 (61.19%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVD-NLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMA-AKNDISRFLVDCPINKSVASTSNKSV 1474
+VL+DMDCFYVQVE K APQY GK AV QYN ++GGG +IAV+YEARA GV R MRGD A + CPDIHLF VPE RGKADLT+YR+AGAEV++ +S++AS +ERAS+DEA++D+TD + ++LV+ DL ++ + +GC +G + S+WL T + LA A LV+++R + GYRCSAGIAH+K AK ACG+HKPNQQT++P + L + +RNLGGK+G ++++L V T+G+++ FS L +TFG+K G WLY L RG D E V R+L +SIGC K F G LNT +KV+YW+ Q+A+E+AER+ D N R + + Q N + + + SR L + I+ A VL+ +NTA + +W PPL ++ L+A KF+ + + DIS+FL P + T +KSV
Sbjct: 51 VVLVDMDCFYVQVEEKLAPQYKGKPCAVSQYNQFKGGG--------------LIAVNYEARAAGVKRGMRGDAAMKLCPDIHLFRVPEVRGKADLTRYRDAGAEVLQVMSQYASIIERASVDEAYLDLTDVLGADMLVAPGDLPNTHVVST--ESHPDGC--IG--ALSEWL------LGTGVDSPEHLLARAAKLVEEIRAAILQKTGYRCSAGIAHNKVLAKLACGLHKPNQQTILPQSAVPGLFVTLPVQNLRNLGGKLGEDIKEKLQVETLGEVARFSQETLVNTFGEKVGSWLYQLVRGIDHEPVSLRKLAKSIGCGKNFNGKLALNTKEKVKYWVQQLAEELAERLQRDKEQNGRSAQLLVIGVRQ----NHASQGTAISRSCQLTTYDSGKIAHDALAVLTRLNTA-----TNENWTPPLCNISLAASKFRDSLGESTQDISKFLSARPAMSTATLTESKSV 484
BLAST of DNA polymerase eta vs. TrEMBL
Match: W4Y5M1 (UmuC domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1) HSP 1 Score: 372.089 bits (954), Expect = 3.268e-114 Identity = 208/504 (41.27%), Postives = 286/504 (56.75%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGY--VSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSL--SGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMAAKN-DISRFLVDCPINKSVA--STSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANE 1561
+++LIDMDCFYVQVE + P GK +AVVQYN+W+GG IIAVSYEARA GV R MRG EA+ KCP I L +VPENRGKADLT+YR AGAEVI L K++ VERAS+DEA++D+TD+V L + D G++ + I G H V V D +E T RL +GA + ++MR V+ G+RCSAGI+H+K AK ACG HKPN+Q+++P + + + I K RNLGGK+G + + L V MG L ++ QL + +KTG WLY +CRG DDE V RQL +S GCSK FPG N L+T DKV++W +A+EV R+ + TN R H+ VS Q + K L + SR + ++++ I+K F V+ +N AG+ A W P + LG+SA KF ++K+ I FL P + + VP + + ++ TLD + S+ E
Sbjct: 4 VVILIDMDCFYVQVEQRRDPTLKGKPAAVVQYNSWKGGA--------------IIAVSYEARAFGVTRQMRGAEARGKCPAIQLVTVPENRGKADLTRYREAGAEVITVLCKYSDSVERASVDEAYVDVTDQVQTRLAQMEDQGQVLDPEQLHQTFIAG-HDVAEEGVDIEDVRKRGLETWLTSADLATKRLGVGALIAEEMRAAVFKDTGFRCSAGISHNKMLAKIACGFHKPNRQSVLPPEGLDKVYKTIPIRKFRNLGGKLGHSISEDLGVEYMGDLLRYTEKQLQKRYSEKTGTWLYSICRGIDDEPVRPRQLAKSTGCSKTFPGKNALDTRDKVKHWFRSLAEEVEHRLLREEETNNRTAKHLTVSVGQ------AGKPLMTTASRSCAIHQISSEKIAKDCFSVIQNLNHAGNHQAA---WSPAITYLGISASKFVEGGSSKSGSIQSFLSSGPSKPATTLLKEDQQEVPSPERAQDQTSRRTLDRFLTQSTKEPKQE 483
BLAST of DNA polymerase eta vs. Ensembl Cavefish
Match: poli (DNA polymerase iota [Source:NCBI gene;Acc:103044286]) HSP 1 Score: 122.094 bits (305), Expect = 1.883e-28 Identity = 125/456 (27.41%), Postives = 200/456 (43.86%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASID-KIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTA----AGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFL 1423
+I+ D+DCFY QVE+ P K V Q ++I I+ +Y AR + V + M EA +KCPD+ L +G+ DLT YR+ +V + L VER DE F+D+++ V+ + +D SI VG+V + ++ E D RLA+G+ + ++RE + + LG AG+A SK AK G KPNQQT + ++LMN+ S K+ +G + + + L V ++ L F L L FG+ G + +L G D V QS+ F ++T+ +V+ + ++ + ER+ +D RLP + ++ R SR SR P+ + TA G +A +LS V H + G F L L + + + + +N IS F
Sbjct: 45 VILHFDLDCFYAQVEMIRNPALRSKPLGVQQ--------------KYI-----IVTCNYVARERSVTKLMGVKEALEKCPDLVLV-----KGE-DLTHYRDISYKVTELLMSFCPLVERLGFDENFVDVSEMVETRM---------------KDTSISDLSFVGHVYGHEA--------SSVEVGDHSRLAVGSAIAAELREALRSRLGLTSCAGVATSKLLAKLVSGAFKPNQQTTLLPQSSAELMNSLSGPCKVPGIGYRTVEKL-KVLGVVSVRDLQLFPLRDLIREFGEVNGRRIQNLACGVDLSPVTPAGPPQSLSDEDSF---KKISTVREVQSKVTELLNSLCERMCKD----GRLPQTVRLTLR--RASAPSRWFSRESRQCPIPKHTAQKIITGCGEAVPQLLSIVMKLFHKLVNAGDPF-HLTLLNVCFSNLQTKNSGRNAISSFF 441
BLAST of DNA polymerase eta vs. Ensembl Cavefish
Match: poli (DNA polymerase iota [Source:NCBI gene;Acc:103044286]) HSP 1 Score: 121.709 bits (304), Expect = 2.431e-28 Identity = 125/456 (27.41%), Postives = 200/456 (43.86%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASID-KIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTA----AGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFL 1423
+I+ D+DCFY QVE+ P K V Q ++I I+ +Y AR + V + M EA +KCPD+ L +G+ DLT YR+ +V + L VER DE F+D+++ V+ + +D SI VG+V + ++ E D RLA+G+ + ++RE + + LG AG+A SK AK G KPNQQT + ++LMN+ S K+ +G + + + L V ++ L F L L FG+ G + +L G D V QS+ F ++T+ +V+ + ++ + ER+ +D RLP + ++ R SR SR P+ + TA G +A +LS V H + G F L L + + + + +N IS F
Sbjct: 31 VILHFDLDCFYAQVEMIRNPALRSKPLGVQQ--------------KYI-----IVTCNYVARERSVTKLMGVKEALEKCPDLVLV-----KGE-DLTHYRDISYKVTELLMSFCPLVERLGFDENFVDVSEMVETRM---------------KDTSISDLSFVGHVYGHEA--------SSVEVGDHSRLAVGSAIAAELREALRSRLGLTSCAGVATSKLLAKLVSGAFKPNQQTTLLPQSSAELMNSLSGPCKVPGIGYRTVEKL-KVLGVVSVRDLQLFPLRDLIREFGEVNGRRIQNLACGVDLSPVTPAGPPQSLSDEDSF---KKISTVREVQSKVTELLNSLCERMCKD----GRLPQTVRLTLR--RASAPSRWFSRESRQCPIPKHTAQKIITGCGEAVPQLLSIVMKLFHKLVNAGDPF-HLTLLNVCFSNLQTKNSGRNAISSFF 427
BLAST of DNA polymerase eta vs. Ensembl Cavefish
Match: polh (polymerase (DNA directed), eta [Source:ZFIN;Acc:ZDB-GENE-060421-4949]) HSP 1 Score: 102.834 bits (255), Expect = 1.161e-22 Identity = 50/98 (51.02%), Postives = 62/98 (63.27%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTK 364
++ L+DMDCFYVQVE + P+ K V QY TW+GGG IIAVSYEARA GV R+M D+AK+ CPD+ + V E+ GKADLTK
Sbjct: 8 VVALVDMDCFYVQVEQRMNPELRHKPCVVAQYKTWKGGG--------------IIAVSYEARAHGVTRNMFADDAKKLCPDLRVARVRESHGKADLTK 91 HSP 2 Score: 93.9745 bits (232), Expect = 7.941e-20 Identity = 57/141 (40.43%), Postives = 77/141 (54.61%), Query Frame = 2
Query: 959 WLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKF 1381
WLY LCRG + E V RQL +SIGCSK F G L T +V++WL Q+A E+ ER+++D N R+ + V +R D R S S SR L A+ ++ + ++ +NTAG A W P L L LSA KF
Sbjct: 144 WLYDLCRGIEFEPVKPRQLPKSIGCSKNFAGKTALATKKQVQHWLHQLALELEERLNKDREMNGRVAKLLTVG---VRQAGDKRLS-SFSRCCALARYEASKMTADSLAIIKSLNTAGSHQEA---WCPALTCLHLSASKF 277
BLAST of DNA polymerase eta vs. Ensembl Cavefish
Match: polh (polymerase (DNA directed), eta [Source:ZFIN;Acc:ZDB-GENE-060421-4949]) HSP 1 Score: 102.834 bits (255), Expect = 1.369e-22 Identity = 50/98 (51.02%), Postives = 62/98 (63.27%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTK 364
++ L+DMDCFYVQVE + P+ K V QY TW+GGG IIAVSYEARA GV R+M D+AK+ CPD+ + V E+ GKADLTK
Sbjct: 8 VVALVDMDCFYVQVEQRMNPELRHKPCVVAQYKTWKGGG--------------IIAVSYEARAHGVTRNMFADDAKKLCPDLRVARVRESHGKADLTK 91 HSP 2 Score: 93.9745 bits (232), Expect = 1.058e-19 Identity = 57/141 (40.43%), Postives = 77/141 (54.61%), Query Frame = 2
Query: 959 WLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKF 1381
WLY LCRG + E V RQL +SIGCSK F G L T +V++WL Q+A E+ ER+++D N R+ + V +R D R S S SR L A+ ++ + ++ +NTAG A W P L L LSA KF
Sbjct: 144 WLYDLCRGIEFEPVKPRQLPKSIGCSKNFAGKTALATKKQVQHWLHQLALELEERLNKDREMNGRVAKLLTVG---VRQAGDKRLS-SFSRCCALARYEASKMTADSLAIIKSLNTAGSHQEA---WCPALTCLHLSASKF 277
BLAST of DNA polymerase eta vs. Ensembl Cavefish
Match: rev1 (REV1 DNA directed polymerase [Source:NCBI gene;Acc:103044976]) HSP 1 Score: 92.4337 bits (228), Expect = 7.748e-19 Identity = 92/396 (23.23%), Postives = 145/396 (36.62%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRG------GGYTICELRFIQ---------------------------------------SVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERV 1126
I+ +DMDCF+V V ++ P+ +GK AV N RG G E+++ Q S+ I + SYEAR GV M +AKQ CP + SVP D Y+ + + L+ + +E S DEA +D T L EL + D L +R DV G S G+ + A+ A KPN Q + + + D + + S+ + +G M + + L V+T G L S+ +L FG +TG L+ CRG DD V S + +S+ + + ++ +L ++ EV +R+
Sbjct: 474 ILHVDMDCFFVSVGIRHRPELIGKPVAVTS-NRGRGVVPQRAGANPQLEMQYYQKKRTQYRAGGADTDIEMTPSPENEETCSNGVEQNDLAALSMAEIASCSYEARKAGVRNGMFFGQAKQLCPGLQ--SVP-----YDFQAYKEVALAMYETLASYTYNIEALSCDEALVDAT----------ALLAELGITPDE-------------------------------------------LASAIRTDVREKTGCSASIGMGSNILLARMATRKAKPNGQYFLRPEEVDDFIRDQSVTSLPGVGRSMSSKLAS-LGVNTCGDLQQVSMVRLQKEFGPRTGQTLFRFCRGLDDRPVRSEKERKSVSAEMNY--NIRFTQDEEAETFLTNLSMEVQKRL 805
BLAST of DNA polymerase eta vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009425.1 (pep scaffold:Pmarinus_7.0:GL478442:69188:73716:-1 gene:ENSPMAG00000008525.1 transcript:ENSPMAT00000009425.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 86.6557 bits (213), Expect = 1.980e-18 Identity = 61/166 (36.75%), Postives = 86/166 (51.81%), Query Frame = 2
Query: 959 WLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLV-DCPINKSVA 1453
WL+ LCRG + E V RQL +SIGCSK F G L+T +KV++WL Q++ E+ ER+S+D N R+ + +R D R SR PL A I+ A +++ NTAG W P + L LSA KF ++ I+ FL D +S+A
Sbjct: 2 WLHDLCRGVEFEPVRPRQLPKSIGCSKNFMGREALDTKEKVQHWLQQLSLELEERLSKDRDMNNRVAKLLTAG---VRLSGDQRY-WGLSRSCPLARYDARRIASDALALIAASNTAG---AQHAEWSPAVTCLYLSATKFSDSASSSGGIASFLTGDASATQSLA 160
BLAST of DNA polymerase eta vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005239.1 (pep scaffold:Pmarinus_7.0:GL477257:209381:216639:1 gene:ENSPMAG00000004738.1 transcript:ENSPMAT00000005239.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 59.3066 bits (142), Expect = 1.872e-9 Identity = 47/165 (28.48%), Postives = 68/165 (41.21%), Query Frame = 2
Query: 62 SNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYN-----TWRGGGYTICELRFIQ---------------------------SVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDIT 460
S+P+I+ +DMDCF+V V ++ P GK AV R G E+++ + S+ I + SYEAR GV M AK CPD+ +VP D YR + + L+ + VE S DEA +D+T
Sbjct: 416 SSPVIMHVDMDCFFVSVGIRDRPDLKGKPVAVTHTQGRGSQRPRPGADPHYEMQYYERKLDRGRTDGRGEGGGGGRVASDTAPMSMAEIASCSYEARRAGVKNGMFFGRAKLLCPDLQ--TVP-----YDFDGYREVAQALYETLASFTNEVEALSCDEAMLDVT 573
BLAST of DNA polymerase eta vs. Ensembl Sea Lamprey
Match: polk (polymerase (DNA directed) kappa [Source:ZFIN;Acc:ZDB-GENE-060526-137]) HSP 1 Score: 53.5286 bits (127), Expect = 1.292e-7 Identity = 70/293 (23.89%), Postives = 110/293 (37.54%), Query Frame = 2
Query: 65 NPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIK-CLSKHASCVERASIDEAFIDITDEVDNLLV----SLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAF-------------------------LVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPN-QQTLVPS-DRISDLMNNASIDKIRNLG 847
N IV +DMD FY VE++ P+ K AV S+ + +Y AR GV +M G AK+ CPD+ + + + KY +V++ L+ + S+DEA++DIT + T + + ++ E + SYS L +T Q A F V++MR + G SAGIA + AK ++KPN Q L P+ D I + ++ I K+ +G
Sbjct: 82 NRTIVHVDMDMFYAAVEMRDFPELKDKPMAV-------------------GSMSMLSTSNYHARRFGVRAAMPGFIAKKLCPDLVICPL-------NFNKYSAVSKQVVREVLADYDPNFLPMSLDEAYLDITAHLAERQAWPESRRTFAAHEGLCQTTDKQTDEANDGASFESYSPEL-----FDSTPPQVGGSENAASGFGASASRNGAPERSSTAVVFGLSVEEAVREMRFRIEQKTGLTASAGIAPNMLLAKVGSDMNKPNGQYRLEPTRDAIRNFVDELPIRKVSGIG 343
BLAST of DNA polymerase eta vs. Ensembl Yeast
Match: RAD30 (DNA polymerase eta; involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; involved in formation of post-replicative damage-induced genome-wide cohesion; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV [Source:SGD;Acc:S000002827]) HSP 1 Score: 81.2629 bits (199), Expect = 1.775e-16 Identity = 108/428 (25.23%), Postives = 172/428 (40.19%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAVV--QYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPD---IH--LFSVPEN----------------------RGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLV-----SLT-DLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLM------------ELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMN--NASIDKIRNLGGKMGTFVQQQLNV--------------STMGQLSAFSLGQLTDTFGDKT------------GPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSE 1132
I IDM+ F+ QVE + KE VV Q+N+ IIAVSY AR G++R EA +KC + IH +F E+ K L YR + +K VERASIDE F+D+ N+L+ LT DL L + R+ I G + + S+ + + E R DDV LALG+ + + +R+ + + LGY S G++ +K K A KP+ QT+V +D + D ++ I LGG +G + L++ GQL F ++ + D++ L+ L RG+ + SR + +S+ +K G + + +D + WL+ E+ R+ +
Sbjct: 26 IAHIDMNAFFAQVEQMRCG--LSKEDPVVCVQWNS-------------------IIAVSYAARKYGISRMDTIQEALKKCSNLIPIHTAVFKKGEDFWQYHDGCGSWVQDPAKQISVEDHKVSLEPYRRESRKALKIFKSACDLVERASIDEVFLDLGRICFNMLMFDNEYELTGDLKLKDALSNIREAFIGGNYDIN--SHLPLIPEKIKSLKFEGDVFNPEGRDLITDWDDVILALGSQVCKGIRDSIKDILGYTTSCGLSSTKNVCKLASNYKKPDAQTIVKNDCLLDFLDCGKFEITSFWTLGGVLGKELIDVLDLPHENSIKHIRETWPDNAGQLKEFLDAKVKQSDYDRSTSNIDPLKTADLAEKLFKLSRGRYGLPLSSRPVVKSMMSNKNLRGKSCNSIVDCIS-WLEVFCAELTSRIQD 429
BLAST of DNA polymerase eta vs. Ensembl Nematostella
Match: EDO42211 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S226]) HSP 1 Score: 303.908 bits (777), Expect = 7.297e-98 Identity = 162/359 (45.13%), Postives = 223/359 (62.12%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGY-VSYSD-----WLDHLMELRATDEQCDDVR-LALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERV 1126
+I L+DMDCFYVQVE + P+ GK AVVQYN W+GGG IIAVSYEARA GV R+MRGDEAKQKCPDI L VP RGKA+L YR+AGAEV++ L+ + ERAS+DEA++D+T+ L +T + E+A + R +IEG + G + SD WL L E +R LA GA + +MR V G+ CSAG+A +K AK CG++KP++QTL+P +++L + K+R+LGGK+G+ + +LNV MG L F + L FG K G LY+LCRG D+E V +RQL +S+ C K FPG L+T +V++W++Q+ +E+ ER+
Sbjct: 7 VIALVDMDCFYVQVEQRRCPELKGKPCAVVQYNAWKGGG--------------IIAVSYEARACGVTRNMRGDEAKQKCPDIALVQVPVLRGKANLAHYRDAGAEVLEVLTTYCDNCERASVDEAYLDLTNSAQKKLSVMT-VPEIAE-EVWRTTAIEGLSSKGSDTTISDELQKEWLSQL-------EASPYLRNLAAGAVIAAEMRHAVLEKTGFTCSAGVATNKVLAKLCCGLNKPDKQTLLPHSAVTNLFKTMPLRKVRHLGGKLGSQLHSELNVEFMGDLLQFKINDLKAQFGSKNGELLYNLCRGIDNEPVRARQLPKSVSCGKNFPGKTKLSTCQQVKFWMEQLVEELHERL 342
BLAST of DNA polymerase eta vs. Ensembl Nematostella
Match: EDO38905 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBP1]) HSP 1 Score: 127.487 bits (319), Expect = 9.080e-32 Identity = 97/365 (26.58%), Postives = 179/365 (49.04%), Query Frame = 2
Query: 65 NPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCH-AVGYVSYSDWLDHLMELRATDEQCD---DVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLG----GKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSED 1135
N +IV ID+DCFY QVE+ P + V Q + ++ +Y ARA G+ + + EAK+KCPD+ + + DLTKYR + V K L + A VER +DE ++D+++ ++++R + G + AVG + + D++++ +++E C RL +G+ + ++R+ +++ +G AGIAH+K AK A +KPN+QTL+ ++R+ LM + K+ +G ++G+ L ++T+ +L S L + F + L+ L G DD V + + +SI + ++L +++ V+ L + + R++ED
Sbjct: 2 NRVIVHIDIDCFYAQVEMIRNPCLRDRPVGVSQKHI-------------------LVTCNYVARAMGLKKLISLAEAKKKCPDLVIIN------GEDLTKYREFSSRVTKLLRRFALRVERLGLDENYVDVSE----------------LVQERMKHAPRGSYIAVGCI----YGDNILK-GSSNEVCPCGCHERLTVGSVIASELRKAIFDEIGLTTCAGIAHNKLLAKIAGEQNKPNKQTLLYTERVEGLMGLLPVRKVPGIGRSTCARLGS-----LGITTIAELQECSKKALLNEFNSQEVRILHALSHGADDSQVSTDSMPKSISEEDSY---SNLCSLESVKTNLRVLIGNLIPRITED 312
BLAST of DNA polymerase eta vs. Ensembl Nematostella
Match: EDO49981 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7REV5]) HSP 1 Score: 80.4925 bits (197), Expect = 1.125e-15 Identity = 97/388 (25.00%), Postives = 153/388 (39.43%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELR---------------------------FIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDI----HLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLN---VSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSE 1132
+I+ IDMDCF+V V ++ P+ GK AV ++N GGG CEL+ F S+ I + SY AR GV M +A++ CPDI +LF KYR + + L ++ +E S DEAF+D+T +D + T+ LA R+ C+A +S + L + C + G F + RED D++ + K ++L G +G + ++LN V T L SL L FG KTG L+ CRG DD + + +S+ + G + V++ ++++A EV +R+ E
Sbjct: 285 VIMHIDMDCFFVSVYVRDHPELDGKPVAVSRFNG--GGG---CELKSSHIVNYEMSFPTGFVADLTSPDKSSAFFNSMSEIASCSYAARECGVRNGMFLGQARKLCPDITCVPYLFD-----------KYRQVSQRLYEILVSYSHDIEAVSCDEAFLDVTHSLD----AGTEPMALAQQIRAEIRAATRCNASAGISSNVLLARM---------CTRIAKPDGQFYLS--RED-----------------------------------------DVIQFMASQKAKDLPG-VGWALSKKLNDMGVETCADLQKLSLQALQKEFGPKTGLSLHRSCRGVDDRPLRVERERKSVSAEINY-GIRFTQDYEGVKF-IEELAGEVHKRLRE 597
BLAST of DNA polymerase eta vs. Ensembl Nematostella
Match: EDO28363 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T6P5]) HSP 1 Score: 66.2402 bits (160), Expect = 2.778e-12 Identity = 42/162 (25.93%), Postives = 81/162 (50.00%), Query Frame = 2
Query: 647 GAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPG--SNHLNTIDKVRYWLDQMAQEVAERV 1126
+ + Q++R+ +++ L R SAGI+ +K AK A ++KPN Q + + + + I+K + GK+ + L ++ +L TFG K+G YH+ RG + V ++ +SI + F S+ + I++ LD++A E+ +R+
Sbjct: 45 ASLIAQEIRQRIWDELTLRASAGISINKFIAKIASDINKPNGQKTINPEEVVSFLEELPINKFFGI-GKVTAAKMHNYGIFKGADLKKWTKEELEATFG-KSGAQYYHIVRGIHNSQVKPNRVRKSIAAERTFTENISSEIYMIER----LDKIAVELEKRM 200
BLAST of DNA polymerase eta vs. Ensembl Nematostella
Match: EDO29212 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T4A2]) HSP 1 Score: 54.299 bits (129), Expect = 1.197e-7 Identity = 72/270 (26.67%), Postives = 104/270 (38.52%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATD-EQCDDVRLALGAFL---------VQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDR--ISDLMNNASIDKIRNLG 847
IV +DMD FY VE++ P K AV S + +Y AR GV +M G AK+ CP HL VP KYR V ++ S+DEA++D+TD L+ E + + R + + G S S+ + A + E V ++G + VQ++R + N SAGIA + AK +KPN Q + DR I + I KI +G
Sbjct: 85 IVHVDMDAFYAAVEMRDDPSLKDKPMAV-------------------GSTSMLSTSNYPARRYGVRAAMPGFIAKKLCP--HLIIVP-----LHFDKYRAVSKIVRGIFREYDPRFVPMSLDEAYLDLTD----YLIERQRKTEKRLFYKKVYR--QTSPSAGVPSESESSEACGSNTAQESENVPSVAESVGEPIEFGNDVKDVVQEIRFRIENETQLTASAGIACNVLLAKVCTDCNKPNGQFYLKPDREEIVSFVKTLPIRKISGIG 322
BLAST of DNA polymerase eta vs. Ensembl Medaka
Match: polh (DNA polymerase eta [Source:NCBI gene;Acc:101174214]) HSP 1 Score: 357.066 bits (915), Expect = 3.123e-113 Identity = 206/500 (41.20%), Postives = 286/500 (57.20%), Query Frame = 2
Query: 71 IIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVG------------YVSYSD---------WLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFL-VDCPINKSVASTSNKSVPIADSNFEDS 1504
++ L+DMDCFYVQVE + P V QY TW+GGG IIAVSYEARA GV R+M D+AK+ CPD+ + V E+ GKADLT YR+A EVI+ +S+ A +ERASIDEA++D+T V L + T+ L + I+G G + Y + W++ L + + C +++L +GA +V++MR V G+RCSAGI+H+K AK ACG++KPN+QT++P + +++L N+ I KIRNLGGKMG + + L V MG L+ FS QL FG+KTG WLY LCRG + E+V RQL +SIGCSK FPG L T ++V+YWL Q+A E+ ER+++D TN R+ + V QL D ++ S SR L AA +S +F ++ +NTAG+ A W PPL L LSA KF + I+ FL +SV++TS S P A +DS
Sbjct: 8 VVALVDMDCFYVQVEQRLNPALRNTPCVVAQYKTWKGGG--------------IIAVSYEARAHGVTRNMWVDDAKKLCPDLQVARVRESHGKADLTHYRDASVEVIEVMSRFA-VIERASIDEAYMDLTAAVQQRLKTTTEEQMSPCL--LKTTYIQGYPQSGLDPPSADASPEEMILYKEELRSKGLQQWMESLPTPSSGELNCAELQLTMGALIVEEMRAAVEKQTGFRCSAGISHNKVLAKLACGLNKPNRQTVLPLESVTELFNSLPIGKIRNLGGKMGASIIETLKVENMGDLTRFSQSQLVQHFGEKTGQWLYDLCRGVESEAVKPRQLPKSIGCSKNFPGKTSLATKEQVQYWLHQLALELEERLNKDKETNGRVAKLLTVGVRQLGD----KRPSSFSRCCALVHYEAAKLSSDSFAIIKSLNTAGNHQAA---WTPPLTLLHLSASKFS-DAPSSGGIAGFLSAGVAPGRSVSATSKVSTPQASELKQDS 482
BLAST of DNA polymerase eta vs. Ensembl Medaka
Match: rev1 (REV1 DNA directed polymerase [Source:NCBI gene;Acc:101172774]) HSP 1 Score: 94.7449 bits (234), Expect = 1.463e-19 Identity = 92/396 (23.23%), Postives = 142/396 (35.86%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAVVQYN-----TWRGGGYTICELRFIQ--------------------------------------SVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSE 1132
I +DMDCF+V V ++ P GK AV R G E ++ Q S+ I + SYEAR GV M +AKQ CP + SVP D Y+ + + L+ + +E S DEA ID + + L +S D L Q +R D+ G S G+ + A+ A KPN Q + S+ + D + + + ++ +G MG + L V+T G L SL QL FG +TG L+ CRG DD V + +S+ + + +++ +L ++ EV +R+ E
Sbjct: 547 IFHVDMDCFFVSVGIRHRPDLKGKPVAVTSNRGHGRVPTRPGANPQVEQQYYQRKHAHPQPEKEGDLLHTSPSQDSPDSYANGVNLDSAALSMAEIASCSYEARQAGVRNGMFFGKAKQLCPSLQ--SVP-----YDFEAYKEVAINMYEILASYTRDIEALSCDEALIDGSALLAELGISPDD-----------------------------------------------------LAQAIRADIKEKTGCSASVGMGSNILLARLATRKAKPNGQYFLKSEEVDDFIRDLPVTRLPGVGHVMGKKLAT-LGVTTCGDLQQVSLSQLQRMFGPRTGQTLFRFCRGLDDRPVRCEKERKSVSAEMNY--NIRFTKVEEAESFLSNLSMEVQKRLRE 879
BLAST of DNA polymerase eta vs. Ensembl Medaka
Match: rev1 (REV1 DNA directed polymerase [Source:NCBI gene;Acc:101172774]) HSP 1 Score: 94.3597 bits (233), Expect = 1.669e-19 Identity = 92/396 (23.23%), Postives = 142/396 (35.86%), Query Frame = 2
Query: 74 IVLIDMDCFYVQVELKAAPQYVGKESAVVQYN-----TWRGGGYTICELRFIQ--------------------------------------SVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSE 1132
I +DMDCF+V V ++ P GK AV R G E ++ Q S+ I + SYEAR GV M +AKQ CP + SVP D Y+ + + L+ + +E S DEA ID + + L +S D L Q +R D+ G S G+ + A+ A KPN Q + S+ + D + + + ++ +G MG + L V+T G L SL QL FG +TG L+ CRG DD V + +S+ + + +++ +L ++ EV +R+ E
Sbjct: 556 IFHVDMDCFFVSVGIRHRPDLKGKPVAVTSNRGHGRVPTRPGANPQVEQQYYQRKHAHPQPEKEGDLLHTSPSQDSPDSYANGVNLDSAALSMAEIASCSYEARQAGVRNGMFFGKAKQLCPSLQ--SVP-----YDFEAYKEVAINMYEILASYTRDIEALSCDEALIDGSALLAELGISPDD-----------------------------------------------------LAQAIRADIKEKTGCSASVGMGSNILLARLATRKAKPNGQYFLKSEEVDDFIRDLPVTRLPGVGHVMGKKLAT-LGVTTCGDLQQVSLSQLQRMFGPRTGQTLFRFCRGLDDRPVRCEKERKSVSAEMNY--NIRFTKVEEAESFLSNLSMEVQKRLRE 888
BLAST of DNA polymerase eta vs. Ensembl Medaka
Match: ENSORLT00000045125.1 (pep primary_assembly:ASM223467v1:9:1943765:1946356:-1 gene:ENSORLG00000024554.1 transcript:ENSORLT00000045125.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 61.2326 bits (147), Expect = 7.984e-11 Identity = 35/87 (40.23%), Postives = 50/87 (57.47%), Query Frame = 2
Query: 212 IQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVD 472
IQ I+ +Y AR +GV + M +AK+KCP++ L RG+ DLT YR A +V + LS VER DE F+D+T+ V+
Sbjct: 14 IQQKYIIVTCNYVARERGVTKLMSVTDAKEKCPELVLV-----RGE-DLTLYREASHQVTELLSSFCPLVERLGFDENFMDVTEMVE 94
BLAST of DNA polymerase eta vs. Planmine SMEST
Match: SMESG000010346.1 (SMESG000010346.1) HSP 1 Score: 1175.23 bits (3039), Expect = 0.000e+0 Identity = 620/639 (97.03%), Postives = 621/639 (97.18%), Query Frame = 2
Query: 56 MKSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANEESNSSLDPPQQHLPALXXXXXXXXXXXXXXXGFFARYMXXXXXXXXXXXXKTQAFXXXXXXXXXXXXXXXKQILMWHAPEHCDWHVAKALQKEISENIYEERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQFFTK 1972
MKSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGG IIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSY+DWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGH DLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLR ANEESNSSLDPPQ HLPALPEPPISPFMPPPKKKGFFARYMENEERIEREEREKTQAFDSPSSSDYYYCDQCCKQILMWHAPEHCDWHVAKALQKEISENIYEERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQF TK
Sbjct: 1 MKSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGG--------------IIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYNDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHRDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRFANEESNSSLDPPQHHLPALPEPPISPFMPPPKKKGFFARYMENEERIEREEREKTQAFDSPSSSDYYYCDQCCKQILMWHAPEHCDWHVAKALQKEISENIYEERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQFLTK 625
BLAST of DNA polymerase eta vs. Planmine SMEST
Match: SMESG000010346.1 (SMESG000010346.1) HSP 1 Score: 1152.5 bits (2980), Expect = 0.000e+0 Identity = 609/628 (96.97%), Postives = 610/628 (97.13%), Query Frame = 2
Query: 89 MDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANEESNSSLDPPQQHLPALXXXXXXXXXXXXXXXGFFARYMXXXXXXXXXXXXKTQAFXXXXXXXXXXXXXXXKQILMWHAPEHCDWHVAKALQKEISENIYEERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQFFTK 1972
MDCFYVQVELKAAPQYVGKESAVVQYNTWRGGG IIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSY+DWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGH DLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLR ANEESNSSLDPPQ HLPALPEPPISPFMPPPKKKGFFARYMENEERIEREEREKTQAFDSPSSSDYYYCDQCCKQILMWHAPEHCDWHVAKALQKEISENIYEERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQF TK
Sbjct: 1 MDCFYVQVELKAAPQYVGKESAVVQYNTWRGGG--------------IIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYNDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHRDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRFANEESNSSLDPPQHHLPALPEPPISPFMPPPKKKGFFARYMENEERIEREEREKTQAFDSPSSSDYYYCDQCCKQILMWHAPEHCDWHVAKALQKEISENIYEERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQFLTK 614
BLAST of DNA polymerase eta vs. Planmine SMEST
Match: SMESG000010346.1 (SMESG000010346.1) HSP 1 Score: 1097.8 bits (2838), Expect = 0.000e+0 Identity = 589/639 (92.18%), Postives = 592/639 (92.64%), Query Frame = 2
Query: 56 MKSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANEESNSSLDPPQQHLPALXXXXXXXXXXXXXXXGFFARYMXXXXXXXXXXXXKTQAFXXXXXXXXXXXXXXXKQILMWHAPEHCDWHVAKALQKEISENIYEERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQFFTK 1972
MKSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGG IIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTK + + I DEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSY+DWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGH DLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLR ANEESNSSLDPPQ HLPALPEPPISPFMPPPKKKGFFARYMENEERIEREEREKTQAFDSPSSSDYYYCDQCCKQILMWHAPEHCDWHVAKALQKEISENIYEERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQF TK
Sbjct: 1 MKSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGG--------------IIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKLSVPMVVAMTPV------------------IADEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYNDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHRDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRFANEESNSSLDPPQHHLPALPEPPISPFMPPPKKKGFFARYMENEERIEREEREKTQAFDSPSSSDYYYCDQCCKQILMWHAPEHCDWHVAKALQKEISENIYEERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQFLTK 607
BLAST of DNA polymerase eta vs. Planmine SMEST
Match: SMESG000010350.1 (SMESG000010350.1) HSP 1 Score: 1072.38 bits (2772), Expect = 0.000e+0 Identity = 579/640 (90.47%), Postives = 581/640 (90.78%), Query Frame = 2
Query: 56 MKSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANEESNSSLDPPQQHLPALXXXXXXXXXXXXXXXGFFARYMXXXXXXXXXXXXKTQAFXXXXXXXXXXXXXXXKQILMWHAPEHCDWHVAKALQKEISENIY-EERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQFFTK 1972
MKSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGG IIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDK+ PWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGH DLADGSWFPPLLSLGLS GKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANEESNSSLDPPQQHLPALPEPPISPFMPPPKKKGFFARYMENEERIEREEREKTQAFDSPSSSDYYYCD CC QI+MWHAPEHCDWHVAKALQKEISENIY EERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQFFTK
Sbjct: 1 MKSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGG--------------IIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKM----------------------------------------PWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHPDLADGSWFPPLLSLGLSVGKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANEESNSSLDPPQQHLPALPEPPISPFMPPPKKKGFFARYMENEERIEREEREKTQAFDSPSSSDYYYCDHCCMQIIMWHAPEHCDWHVAKALQKEISENIYEEERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQFFTK 586
BLAST of DNA polymerase eta vs. Planmine SMEST
Match: SMESG000010350.1 (SMESG000010350.1) HSP 1 Score: 1051.2 bits (2717), Expect = 0.000e+0 Identity = 568/629 (90.30%), Postives = 570/629 (90.62%), Query Frame = 2
Query: 89 MDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELRFIQSVLRIIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKIRNLGGKMGTFVQQQLNVSTMGQLSAFSLGQLTDTFGDKTGPWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHCDLADGSWFPPLLSLGLSAGKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANEESNSSLDPPQQHLPALXXXXXXXXXXXXXXXGFFARYMXXXXXXXXXXXXKTQAFXXXXXXXXXXXXXXXKQILMWHAPEHCDWHVAKALQKEISENIY-EERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQFFTK 1972
MDCFYVQVELKAAPQYVGKESAVVQYNTWRGGG IIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDK+ PWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGH DLADGSWFPPLLSLGLS GKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANEESNSSLDPPQQHLPALPEPPISPFMPPPKKKGFFARYMENEERIEREEREKTQAFDSPSSSDYYYCD CC QI+MWHAPEHCDWHVAKALQKEISENIY EERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQFFTK
Sbjct: 1 MDCFYVQVELKAAPQYVGKESAVVQYNTWRGGG--------------IIAVSYEARAKGVNRSMRGDEAKQKCPDIHLFSVPENRGKADLTKYRNAGAEVIKCLSKHASCVERASIDEAFIDITDEVDNLLVSLTDLGELAMLKDRRDRSIEGCHAVGYVSYSDWLDHLMELRATDEQCDDVRLALGAFLVQQMREDVYNSLGYRCSAGIAHSKTTAKFACGVHKPNQQTLVPSDRISDLMNNASIDKM----------------------------------------PWLYHLCRGQDDESVLSRQLTQSIGCSKRFPGSNHLNTIDKVRYWLDQMAQEVAERVSEDCSTNARLPTHMCVSYHQLRDGNDSRKSLSGSRVVPLKELTAAGISKAAFVVLSGVNTAGHPDLADGSWFPPLLSLGLSVGKFKPQMAAKNDISRFLVDCPINKSVASTSNKSVPIADSNFEDSNSVTLDSIASDSSLRCANEESNSSLDPPQQHLPALPEPPISPFMPPPKKKGFFARYMENEERIEREEREKTQAFDSPSSSDYYYCDHCCMQIIMWHAPEHCDWHVAKALQKEISENIYEEERKSRLAKQNQNSKLKRKQISKPSKSNQLKIDQFFTK 575 The following BLAST results are available for this feature:
BLAST of DNA polymerase eta vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of DNA polymerase eta vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 1
BLAST of DNA polymerase eta vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of DNA polymerase eta vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of DNA polymerase eta vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of DNA polymerase eta vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of DNA polymerase eta vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of DNA polymerase eta vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DNA polymerase eta vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of DNA polymerase eta vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 3
BLAST of DNA polymerase eta vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of DNA polymerase eta vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of DNA polymerase eta vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 4
BLAST of DNA polymerase eta vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30034709 ID=SMED30034709|Name=DNA polymerase eta|organism=Schmidtea mediterranea sexual|type=transcript|length=2011bpback to top protein sequence of SMED30034709-orf-1 >SMED30034709-orf-1 ID=SMED30034709-orf-1|Name=SMED30034709-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=640bp MKSNPIIVLIDMDCFYVQVELKAAPQYVGKESAVVQYNTWRGGGYTICELback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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