GCR158

Overview
NameGCR158
Smed IDSMED30034659
Length (bp)1116
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GCR158 (SMED30034659) t-SNE clustered cells

Violin plots show distribution of expression levels for GCR158 (SMED30034659) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GCR158 (SMED30034659) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GCR158 (SMED30034659) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30034659

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30034659SMESG000077660.1 dd_Smed_v4_6018_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30034659SMESG000077660.1 dd_Smed_v4_6018_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30034659SMESG000077660.1 dd_Smed_v4_6018_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30034659SMESG000077660.1 SMESG000077659.1 Contig38439newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30034659SMESG000077660.1 SMESG000077659.1 Contig38439uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30034659SMESG000077660.1 SMESG000077659.1 Contig19746newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30034659SMESG000077660.1 SMESG000077659.1 Contig19746uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of GCR158 vs. TrEMBL
Match: A0A193KUR6 (GCR156 OS=Schmidtea mediterranea OX=79327 GN=gcr156 PE=2 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 3.005e-15
Identity = 64/238 (26.89%), Postives = 113/238 (47.48%), Query Frame = 1 
Query:  331 IAVDGYLAVRDFSISSQMTIFVAIAIDRLLTLTHPQITKRRTNTIIKVLQTAVFFIGLFYLLRFIFLTIDIKCNSKVLLDKYMKLFIGIVGGVLMTTNLIIYLVLFGLLCHRQ----RKESRKFQKSKKLLRTQXXXXXXXXXXXXXXXIPMRPTXXXXXXXXXXXXXXXXXASIRFAKPLFAINLLMIISYLPAILYNVRFVGFHSYSVYYLFFLHALGDPILFFSTRPELKLALLR 1032
            I ++G+L++R+  IS      + +A +R   + HPQ+     + +IK+ +     I + +L+RF  +     C     +D  +K+ IGI   +++   + IY VLFGLLC R      K +  F K       +                        +S+   K        S++FA+ LF+I +LM+ISY P  L NV  + +   ++YYLF+L  LGDP++   +R +++  ++R
Sbjct:   93 ILLEGFLSLRNCLISCHAITLLFMATNRYRAIKHPQMVNDPRSVLIKLYRIIYVTISIPFLVRFALVFCLRDCLLLQEIDNALKMSIGIATALVVMVIICIYSVLFGLLCCRNFPSTSKPTGNFSKIHPYPNER------------------------KSTTVGKVFKRHFGKSVKFAQVLFSITILMMISYAPVFLTNVNLIKYPGQAIYYLFYLPVLGDPLILILSREDIRQEMIR 306
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BLAST of GCR158 vs. TrEMBL
Match: A0A193KUF9 (GCR158 OS=Schmidtea mediterranea OX=79327 GN=gcr158 PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 7.764e-15
Identity = 106/329 (32.22%), Postives = 186/329 (56.53%), Query Frame = 1 
Query:   61 MITNNTCRSHDVKKEDIEVAFFPLYASMFAVAVILNSVLLHLVVKRNRLFLEKRTFDKLFPVXXXXXXXXXXXXXXXXXXKKYTNDTDTYIAVDGYLAVRDFSISSQMTIFVAIAIDRLLTLTHPQITKRRTN--TIIKVLQTAVFFIGLFYLLRFIFLTIDIKCNSKVLLDKYMKLFIGIVGGVLMTTNLIIYLVLFGLLCHRQRKESRKFQKSKKLLRTQXXXXXXXXXXXXXXXIPMRPTXXXXXXXXXXXXXXXXXASIRFAKPLFAINLLMIISYLPAILYNVRFVGFHSYSVY-YLFFLHALGDPILFFSTRPELKLALLRCL 1038
            M++N+T    D   ++ E   F ++  +F ++V LNS+LL+LV+K+N    ++++   LF +LI  DL+ISILILP+I +KK   +   + AV  + A+RD  IS Q+ + VAIAID L+++ +P  +++  N   I ++  T +  I +  L++ I L  ++  N K  ++  +K  + ++G + +   +IIY  LF L+  RQ     K  K K+ L++Q++E   N +            + +      ++   K+   ++F K LF +NLLMI+S+LP  LYN   + F +Y+ + YLF++H+LGD I+F   R +L+  L + L
Sbjct:    1 MLSNSTT---DANIKEGEAIIFSIFIIIFLLSVFLNSILLYLVMKKNYFKFDQQSIHYLFQMLIVCDLLISILILPDILVKKTVTNGKYHPAVILFRAMRDALISIQIFLLVAIAIDGLVSILNPINSRQFWNLSNIKRIFITYLSVIVVLLLVKSILLLSNLDSNVKQNINYAIKFGLSLIGMIFIVMIIIIYSYLFCLIYRRQMDRCDKSMKRKQCLKSQSTETEENFS---------EDESGRNKITVKQWNFGKRIIHMKFVKVLFWVNLLMIVSFLPGCLYNSGLLKFKAYNWFIYLFYVHSLGDAIIFLFIRLDLRKDLTKLL 317
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BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000077660.1 (SMESG000077660.1)

HSP 1 Score: 539.65 bits (1389), Expect = 0.000e+0
Identity = 330/331 (99.70%), Postives = 331/331 (100.00%), Query Frame = 1 
Query:   61 MITNNTCRSHDVKKEDIEVAFFPLYASMFAVAVILNSVLLHLVVKRNRLFLEKRTFDKLFPVXXXXXXXXXXXXXXXXXXKKYTNDTDTYIAVDGYLAVRDFSISSQMTIFVAIAIDRLLTLTHPQITKRRTNTIIKVLQTAVFFIGLFYLLRFIFLTIDIKCNSKVLLDKYMKLFIGIVGGVLMTTNLIIYLVLFGLLCHRQRKESRKFQKSKKLLRTQXXXXXXXXXXXXXXXIPMRPTXXXXXXXXXXXXXXXXXASIRFAKPLFAINLLMIISYLPAILYNVRFVGFHSYSVYYLFFLHALGDPILFFSTRPELKLALLRCLCIKKS 1053
            MITNNTCRSHDVKKEDIEVAFFPLYASMFAVAVILNSVLLHLVVK+NRLFLEKRTFDKLFPVLIFLDLIISILILPNIFIKKYTNDTDTYIAVDGYLAVRDFSISSQMTIFVAIAIDRLLTLTHPQITKRRTNTIIKVLQTAVFFIGLFYLLRFIFLTIDIKCNSKVLLDKYMKLFIGIVGGVLMTTNLIIYLVLFGLLCHRQRKESRKFQKSKKLLRTQNSEENVNENQENLIEIPMRPTNNKRSSLKNKFKSNKKSASIRFAKPLFAINLLMIISYLPAILYNVRFVGFHSYSVYYLFFLHALGDPILFFSTRPELKLALLRCLCIKKS
Sbjct:    1 MITNNTCRSHDVKKEDIEVAFFPLYASMFAVAVILNSVLLHLVVKKNRLFLEKRTFDKLFPVLIFLDLIISILILPNIFIKKYTNDTDTYIAVDGYLAVRDFSISSQMTIFVAIAIDRLLTLTHPQITKRRTNTIIKVLQTAVFFIGLFYLLRFIFLTIDIKCNSKVLLDKYMKLFIGIVGGVLMTTNLIIYLVLFGLLCHRQRKESRKFQKSKKLLRTQNSEENVNENQENLIEIPMRPTNNKRSSLKNKFKSNKKSASIRFAKPLFAINLLMIISYLPAILYNVRFVGFHSYSVYYLFFLHALGDPILFFSTRPELKLALLRCLCIKKS 331
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BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062328.1 (SMESG000062328.1)

HSP 1 Score: 254.603 bits (649), Expect = 3.307e-82
Identity = 168/330 (50.91%), Postives = 217/330 (65.76%), Query Frame = 1 
Query:   73 NTCRSHDVKKEDIEVAFFPLYASMFAVAVILNSVLLHLVVKRNRLFLEKRTFDKLFPVXXXXXXXXXXXXXXXXXXKKYTNDTDTYIAVDGYLAVRDFSISSQMTIFVAIAIDRLLTLTHPQITKRRTNTIIKVLQTAVFFIGLFYLLRFIFLTIDIKCNSKVLLDKYMKLFIGIVGGVLMTTNLIIYLVLFGLLCHRQ---RKESRKFQKSKKL-LRTQXXXXXXXXXXXXXXXIPMRPTXXXXXXXXXXXXXXXXXASIRFAKPLFAINLLMIISYLPAILYNVRFVGFHSYSVYYLFFLHALGDPILFFSTRPELKLALLRCLCIKK 1050
            NTCR HD++K  I+ AF+P+Y+ MF ++V LNS+LL++V+K+++   EK++F KLF VLIFLDLIISILILPNI IK Y+ D DT+I +DGYLA+RDF+ISSQM I V IAIDRLL    P     R   ++KV +       LFY +RF  LT++I C+ K  +D  +KLFIGI GG+LM  NLIIY +LF LLC RQ    K+S      K L ++   S+       +      M   ++          +N+K  SI+F K LF +NLLMIISYLPAILYN   + F S+SVYYLFFLHALGDPILFF TR EL  +LL+ + IK 
Sbjct:   13 NTCRKHDIEKNGIDGAFYPIYSIMFVLSVFLNSILLYIVIKKDKFIHEKKSFHKLFQVLIFLDLIISILILPNILIKMYSKDHDTFIVLDGYLAIRDFAISSQMIILVLIAIDRLLAFLLPLSNFWRIGRVLKVFKIFFILTLLFYSIRFTLLTVNINCDIKKSIDSDVKLFIGIFGGILMVINLIIYCILFALLCRRQIFNHKDSLLSANFKSLNVKELESKSIPKIENKKNSSNAMLLQSDFSQVKFKVIPANRKIGSIKFVKVLFGVNLLMIISYLPAILYNAECIQFPSFSVYYLFFLHALGDPILFFMTRRELLNSLLKIVHIKN 342
[Close]
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062349.1 (SMESG000062349.1)

HSP 1 Score: 229.18 bits (583), Expect = 1.707e-72
Identity = 162/321 (50.47%), Postives = 207/321 (64.49%), Query Frame = 1 
Query:   76 TCRSHDVKKEDIEVAFFPLYASMFAVAVILNSVLLHLVVKRNRLFLEKRTFDKLFPVXXXXXXXXXXXXXXXXXXKKYTNDTDTYIAVDGYLAVRDFSISSQMTIFVAIAIDRLLTLTHPQITKRRTNTIIKVLQTAVFFIGLFYLLRFIFLTIDIKCNSKVLLDKYMKLFIGIVGGVLMTTNLIIYLVLFGLLCHRQRKESRKFQKSKKLLRTQXXXXXXXXXXXXXXXIPMRPTXXXXXXXXXXXXXXXXXASIRFAKPLFAINLLMIISYLPAILYNVRFVGFHSYSVYYLFFLHALGDPILFFSTRPELKLALLRCL 1038
            TCR+HD  K+ IE+AF+P+Y+ MF ++V+LNS+LL++V K      EKR F KLF  LIFLDLIISILILPNI IK++T DTDT I +D +LA+RD SIS+QM   V IAIDRL    +P       NT++ +L+  + F+ L Y+ R I L+I I C +K  +DKY K+ IGI GGV M  NL+IY+ LF LLC RQ    R    S K  R   S+      +EN   I    +    S    K +  ++  SI+FAK L  IN+LMI+SYLP +LYN +F+ F SYSVYYLFFLHALGDPI+F  TR ELK  LL  L
Sbjct:    6 TCRNHDTNKKTIEIAFYPIYSGMFILSVLLNSILLYVVTKIESYAHEKRVFHKLFQFLIFLDLIISILILPNILIKRFTEDTDTNIFLDVFLAIRDLSISAQMITLVLIAIDRLFAFVNPLSNYWVLNTVMWILKVLMLFLSLSYITRLILLSIGISCLNKKKIDKYGKIIIGISGGVFMLINLVIYVSLFSLLCQRQLVRQRNSFNSDKFYRAMQSKSYDENPEENETSIT--KSRRISSLNSRKLRFRRRIGSIKFAKVLLGINILMIVSYLPGVLYNNKFIPFPSYSVYYLFFLHALGDPIIFLLTRRELKNGLLSIL 324
[Close]
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000012027.1 (SMESG000012027.1)

HSP 1 Score: 121.324 bits (303), Expect = 2.280e-31
Identity = 107/326 (32.82%), Postives = 172/326 (52.76%), Query Frame = 1 
Query:   73 NTCRSHDVKKEDIEVAFFPLYASMFAVAVILNSVLLHLVVKRNRLFLEKRTFDKLFPVXXXXXXXXXXXXXXXXXXKKYTNDTDTYIAVDGYLAVRDFSISSQMTIFVAIAIDRLLTLTHPQITKRRTNTIIKVLQTAVFFIGLFYLLRFIFLTIDIKCNSKVLLDKYMKLFIGIVGGVLMTTNLIIYLVLFGLLCHRQRKESRKFQKSKKLLRTQXXXXXXXXXXXXXXXIPMRPTXX-------XXXXXXXXXXXXXXXASIRFAKPLFAINLLMIISYLPAILYNVRFVGFHSYSVY----YLFFLHALGDPILFFSTRPELK 1017
              C+ +  +  + +  F  ++  +F ++V LNS LL LV KRN    +K+   KLF +LI LDL+ISILILPN+ IK++  D  T++AVD + +VRD  IS Q+ + + IA+D L+++ +P  T    N+I K   +    I L ++++ +   +++ C     +++Y KL +G++GG++M T L IY+ LF L+  +Q     KF K +                     +P+R                  K    +K   ++F K LF +NLLM+IS+LP  LYN    G   YS +    YLF++H+LGD I+F   R  +K
Sbjct:    6 EECQRNVTESVESKTVFASIFIFIFLLSVTLNSFLLVLVTKRNYFDFDKQASHKLFQILIALDLVISILILPNVLIKRFAKDP-THLAVDVFRSVRDSVISIQVFLLLLIAVDGLVSILYPIQTFWNLNSITKTFASYSVVISLIFIVKLVVFNVNLSCGLVKKINRYFKLTMGVIGGIIMATILSIYVCLFVLIFRQQFDRHVKFPKQRP-----------------TSPLPVRRNEERNFQDQRTSKFGLEKLMLGQKILQMKFVKVLFLVNLLMVISFLPVSLYN---SGLLRYSYFKWASYLFYIHSLGDAIIFLFVRFNMK 310
[Close]
BLAST of GCR158 vs. Planmine SMEST
Match: SMESG000062345.1 (SMESG000062345.1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.350e-24
Identity = 82/185 (44.32%), Postives = 106/185 (57.30%), Query Frame = 1 
Query:   70 NNTCRSHDVKKEDIEVAFFPLYASMFAVAVILNSVLLHLVVKRNRLFLEKRTFDKLFPVXXXXXXXXXXXXXXXXXXKKYTNDTDTYIAVDGYLAVRDFSISSQMTIFVAIAIDRLLTLTHPQITKRRTNTIIKVLQTAVFFIGLFYLLRFIFLTIDIKCNSKVLLDKYMKLFIGIVGGVLMTTN 624
            N  CR  D  K                  V+ NS+LL++V  R +   +KRTF KLF +LI LDLIIS+LILPNI IK +T DTDT+   D +LA RDF+ISSQM   V IAIDRLL    P     +   ++ +L+  +  I L Y +RFI LTI+I C+ K  +D + K  IGI GG + T N
Sbjct:    9 NGICRKEDTMK------------------VLFNSLLLYVVTNRVKFCHDKRTFHKLFQILIILDLIISLLILPNIIIKGFTKDTDTHWYTDLFLASRDFAISSQMITLVLIAIDRLLAFFVPMSKIWKLEIVMWILEVLLVIIFLSYGVRFILLTINISCSMKKRIDTHGKDIIGISGGDICTIN 175
The following BLAST results are available for this feature:
BLAST of GCR158 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
A0A193KUR63.005e-1526.89GCR156 OS=Schmidtea mediterranea OX=79327 GN=gcr15... [more]
A0A193KUF97.764e-1532.22GCR158 OS=Schmidtea mediterranea OX=79327 GN=gcr15... [more]
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BLAST of GCR158 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR158 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000077660.10.000e+099.70SMESG000077660.1[more]
SMESG000062328.13.307e-8250.91SMESG000062328.1[more]
SMESG000062349.11.707e-7250.47SMESG000062349.1[more]
SMESG000012027.12.280e-3132.82SMESG000012027.1[more]
SMESG000062345.11.350e-2444.32SMESG000062345.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034659 ID=SMED30034659|Name=GCR158|organism=Schmidtea mediterranea sexual|type=transcript|length=1116bp
TTAACCTTTGATTGAAATATAATCAACACAATGAATGTTTGACAAAAATT
ATTCCACACAATGATTACAAATAATACTTGTCGAAGTCACGATGTGAAAA
AGGAGGATATCGAGGTTGCGTTCTTCCCCTTATACGCCTCAATGTTCGCA
GTAGCCGTAATATTAAATTCAGTTCTTCTACATTTGGTTGTCAAAAGAAA
TAGACTGTTTCTCGAGAAGAGAACATTTGATAAATTGTTTCCAGTTCTGA
TTTTTCTGGATTTGATAATTTCGATTTTGATTTTACCGAATATTTTCATC
AAGAAATATACAAACGACACTGATACCTATATTGCAGTTGACGGGTATTT
AGCAGTTCGAGACTTTTCAATATCAAGCCAAATGACAATTTTCGTTGCAA
TCGCAATAGATCGGCTATTAACTCTCACCCATCCGCAGATTACAAAACGA
AGAACAAACACGATTATTAAGGTCTTACAAACCGCAGTCTTTTTCATCGG
TCTATTCTATCTCTTACGATTCATTTTTCTTACAATCGACATCAAATGTA
ACAGTAAGGTATTATTGGATAAATATATGAAACTATTTATTGGTATCGTC
GGTGGAGTTTTAATGACAACGAATTTAATTATTTATCTCGTTTTGTTCGG
ACTTTTATGTCATCGTCAAAGAAAAGAAAGCAGAAAATTTCAGAAATCAA
AGAAATTATTGAGAACACAGAACTCAGAGGAAAATGTCAACGAAAACCAA
GAGAATCTAATTGAAATACCTATGAGACCTACGAATAATAAAAGATCCTC
GTTAAAAAATAAATTTAAATCAAATAAAAAAAGTGCCTCTATCAGATTTG
CGAAACCTCTTTTTGCAATAAACCTTTTAATGATAATTTCATACCTACCA
GCTATTTTGTACAATGTTAGATTTGTTGGTTTTCATTCATATTCTGTATA
TTATTTATTCTTTCTCCATGCTTTGGGTGATCCGATTCTATTTTTTTCAA
CTCGACCGGAATTGAAACTTGCATTACTGAGATGCTTGTGTATTAAAAAG
TCGTGATAAATATTGATTGTTTACACAGTTTCATAATCTAAATAAACAAA
TTTGAATGAAAAAAAA
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protein sequence of SMED30034659-orf-1

>SMED30034659-orf-1 ID=SMED30034659-orf-1|Name=SMED30034659-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=332bp
MITNNTCRSHDVKKEDIEVAFFPLYASMFAVAVILNSVLLHLVVKRNRLF
LEKRTFDKLFPVLIFLDLIISILILPNIFIKKYTNDTDTYIAVDGYLAVR
DFSISSQMTIFVAIAIDRLLTLTHPQITKRRTNTIIKVLQTAVFFIGLFY
LLRFIFLTIDIKCNSKVLLDKYMKLFIGIVGGVLMTTNLIIYLVLFGLLC
HRQRKESRKFQKSKKLLRTQNSEENVNENQENLIEIPMRPTNNKRSSLKN
KFKSNKKSASIRFAKPLFAINLLMIISYLPAILYNVRFVGFHSYSVYYLF
FLHALGDPILFFSTRPELKLALLRCLCIKKS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 39..286
e-value: 1.2E-12
score: 47.8
NoneNo IPR availablePANTHERPTHR24246OLFACTORY RECEPTOR AND ADENOSINE RECEPTORcoord: 17..327
NoneNo IPR availableCDDcd149817tmA_Prostanoid_Rcoord: 102..215
e-value: 0.00143078
score: 37.6102
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 17..330
NoneNo IPR availableTMHMMTMhelixcoord: 21..43
NoneNo IPR availableTMHMMTMhelixcoord: 294..313
NoneNo IPR availableTMHMMTMhelixcoord: 267..289
NoneNo IPR availableTMHMMTMhelixcoord: 58..80
NoneNo IPR availableTMHMMTMhelixcoord: 177..199
NoneNo IPR availableTMHMMTMhelixcoord: 135..157
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 35..311
score: 10.963