SMED30034644
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30034644 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30034644 aligns in the following genomic locations:
Homology
BLAST of SMED30034644 vs. Planmine SMEST
Match: SMESG000040931.1 (SMESG000040931.1) HSP 1 Score: 781.171 bits (2016), Expect = 0.000e+0 Identity = 419/431 (97.22%), Postives = 422/431 (97.91%), Query Frame = 2 Query: 215 MKIVILGMISCNIINAVIVIGFFIWTIRKMKPNFRNPANNFLFYSLSIPLFIVSIVFLMLIPTNCLMELYNNXXXXXXXXXXXXXXXXKNLTGFNEINDICQNSVVFITLIGIINHFFFHLIAFHTYSFIEMPPNISLYSQIGYSVKQYDNFLLSNRVLFEGFLFLFCFVCIAHLKSHKFINKSWSISNSQMVSFSQANEENQNFVFTSKVPSLAILSAIVYLIVTLLAIVIVHVSHDFGFRGDSCILSTIFPVPAYLFSIYLMILQMKEMAIWRKRKKDEMSIDIKIMWIGVFGQFLVIFINIFNVHTDEAISSIQLIEYCIMLFQIIFQTVVIGVTLNLEWQPAKKESKLWELLHPPVGLLSMNLSFFLFTIYILLTNLCTNPAAKSSMGNNLIKLGINSISQPLAILHRLHSSFIFGKLLYHTSQKRY 1507 MKIVI GMISCNIINAVIVIGFFIWTIRKMKPNFRNPANNFLFYSLSIPLFIVSIVFLM IPTNCLMELYNNILEIVSLSS+MLLIVLKNLTGFNEINDICQNSVVFITLIGIINHFFFHLIAFHTYSFIEMPPNISLYSQIGYSVKQYDNF SNRVLFEGFLFLFCFVCIAHL SHKFINKS SISNSQMVSFSQANEENQNFVFTSKVPSLAILSAIVYLIVTLLAI+IV VSH FGFRGDSCILSTIFPVPAYLFSIYLMILQMKEMAIWRKRKKDEMSIDIKIMWIGVFGQFL+IFINIFNVHTDEAISSIQLIEYCIMLFQIIFQTVVIGVTLNLEWQPAKKESKLWELLHPPVGLLSMNLSFFLFTIYILLTN CTNPAAKSSMGNNLIKLGINSISQPLAILHRLHSSFIFGKLLYHTSQKRY Sbjct: 1 MKIVISGMISCNIINAVIVIGFFIWTIRKMKPNFRNPANNFLFYSLSIPLFIVSIVFLMSIPTNCLMELYNNILEIVSLSSNMLLIVLKNLTGFNEINDICQNSVVFITLIGIINHFFFHLIAFHTYSFIEMPPNISLYSQIGYSVKQYDNFWYSNRVLFEGFLFLFCFVCIAHLVSHKFINKSSSISNSQMVSFSQANEENQNFVFTSKVPSLAILSAIVYLIVTLLAIIIVQVSHYFGFRGDSCILSTIFPVPAYLFSIYLMILQMKEMAIWRKRKKDEMSIDIKIMWIGVFGQFLLIFINIFNVHTDEAISSIQLIEYCIMLFQIIFQTVVIGVTLNLEWQPAKKESKLWELLHPPVGLLSMNLSFFLFTIYILLTNFCTNPAAKSSMGNNLIKLGINSISQPLAILHRLHSSFIFGKLLYHTSQKRY 431
BLAST of SMED30034644 vs. Planmine SMEST
Match: SMESG000040891.1 (SMESG000040891.1) HSP 1 Score: 169.859 bits (429), Expect = 2.296e-46 Identity = 153/524 (29.20%), Postives = 257/524 (49.05%), Query Frame = 2 Query: 74 MKSHFENLIFLNIVVTVSLLYFSFGFYFNFSWACWQLDYKSNETEKYMK-----IVILGMISC-NIINAVIVIGFFIWTIRKMKPNFRNPANNFLFYSLSIPLFIVSIVFLMLIPTNCLMELYNNXXXXXXXXXXXXXXXXKNLTGFNEIN-----------DICQNSVVFITLIGIINHFFFHLIAFHTYSFIEMPPNISLYSQI-GYSVKQYDNFLLSNRV--LFEGFLFLFCFVCIAHL--------KSHKFINK-SWSISNSQMVSFSQANEEN----QNFVFTS--------KVPSLAILSAIVYLIVTLLAIVIVHV---SHDFGFRGDSCILSTIFPVPAYLFSIYLMILQMKEMAIWRKRKKDEMSIDIKIMWIGVFGQFLVIF--INIFNVHTDEAISSIQLIEYCIMLFQIIFQTVVIGVTLNLEWQPAKKESKLW-ELLHPPVGLLSMNLSFFLFTIYILLTNLCTNPAAKSSMGNNLIKL---GINSISQPLAILHRLHSSFIFGKLLYHTS 1495 M ++ IF ++ L YF GF+ + WAC +LD K + E+ V+ G+I+C N I +I I ++ RN N LFY L PLF+ +IVF +L T M+L+ N L ++ M L + N IN + V+ + IGIIN+F FH+ +FI + + YS+ S K + +R+ + + LFC C+ +L S K ++ S +S + + + EN +N V S ++P L+++ I+ + V+LLA+ I++ S F G LSTIFP+P + S++L+ +Q+ EM W K KK+E +D ++WIG GQ + F + ++ D + + + Y +++ Q I QT+ I ++L+ EW E K W E + P VG+LS+N+SF + ++IL+ ++ AK+++ +K+ G +S+ + L IL RLHSS IF K++ +T+ Sbjct: 1 MNPEYKYEIFEKLLQLTGLFYFVSGFFTTYCWACKELD-KVSPHEQINNFWNPIAVVSGIINCFNFILTIITIS----QGKRQVQTERNIEGNLLFYVLCAPLFLATIVFTILEITVFSMKLHENDLTMLMSIGEMFLFIGTFFLILN-INFKFWIFLGSKMKLLNRFVMLTSFIGIINYFMFHMTDQQITNFISIASDYDAYSKYYNNSTKPREGPFHFSRIKKYVQPLVILFCCTCMVYLIKFYFSRTSSQKNLDDNSTELSEETLDKINDSETENLIIRENEVVRSNTKNPPSFRLPPLSLVLGILLITVSLLAVAILNSLLKSTSLYFTGQ--YLSTIFPIPIDILSLFLLYMQIIEMERWPKIKKNENIVDSTLVWIGAIGQITIAFSQLELYTFIRDPGLVAAKDCYYFVLILQTILQTLTISLSLDREW--ISPERKSWQEYICPAVGILSINISFLIINVFILMGDVLNEQMAKNNITELFLKMFGNGFSSLGETLGILQRLHSSLIFIKIIIYTN 514
BLAST of SMED30034644 vs. Planmine SMEST
Match: SMESG000020517.1 (SMESG000020517.1) HSP 1 Score: 166.777 bits (421), Expect = 2.932e-45 Identity = 146/494 (29.55%), Postives = 247/494 (50.00%), Query Frame = 2 Query: 128 LLYFSFGFYFNFSWACWQLDYKSN--ETEKYMKIVILGMISCNIINAVIVIGFFIWTIRKMKPNFRNPANNFLFYSLSIPLFIVSIVFLMLIPTNCLMEL--YNNXXXXXXXXXXXXXXXXKNLTGF--------NEINDICQNSVVFITLIGIINHFFFHLIAFHTYSFIEMP---PNISLYSQIGYSVKQYDN--FLLSNRVLFEGFLFLFCFVCIAHL-----KSHKFINKSWSISNSQMVSFSQANEE------NQNFVFTSKV------PSLAILSAIVYLIVTLLAIVIVH-VSHDFGFRGDSCILSTIFPVPAYLFSIYLMILQMKEMAIWRKRKKDEMSIDIKIMWIGVFGQFLVIF--INIFNVHTDEAISSIQLIEYCIMLFQIIFQTVVIGVTLNLEWQPAKKESKLW-ELLHPPVGLLSMNLSFFLFTIYILLTNLCTNPAAKSSMGNNLIKL---GINSISQPLAILHRLHSSFIFGKLLY 1486 LLYF GF N+ W C +LD KS+ ++ + + + ++ V V+ F W++ K K + +N L Y++ IP F+ +IV+ + ME N+ + + L I + L F N I + VV + + GI+N +H+ +FI++ P +S + + S + DN L+ + F+ LFCFV +++L +H I ++ + ++ + A+EE N F+ KV P ++ AI+++ VT I I + + + F + ++S+IFP+P YLFSI+L+I+QM ++ W K D+ ID I+W+G GQ + I I++ + +S + EY I+ FQ +FQT+VI + L+ +W K ES W E L PPVG+LS+NLSF +F+++ ++ N + +++ G S LAIL RLHSS IFGK+++ Sbjct: 18 LLYFVMGFLVNYLWICKELDMKSDFKRILEFYNPIAMATSLMDFVSLVSVLILFKWSVSKKKTDLEKLESNILLYTIVIPTFLATIVYSSVKVILSAMEFSEKNDFILFSVMGEICLFISVIILIQFFLKSKFIVNYFQRIYIDFVVLLNIFGIVNLICYHMSVQQINNFIKIASKFPTLSKFYNLSIS-QPTDNESSLIKINSFLQPFVILFCFVILSYLILFYNSAHYEIQETEKLLIDEINLRTGASEEDIIASSNPTISFSFKVFQSINLPPFSLGLAILFITVTFFIIAIANNMMNSPKFATEGQLISSIFPIPIYLFSIFLLIIQMNDICRWEKLGTDKYLIDRMILWVGATGQVVEIVAQISMNSYQYYPKLSGWIIAEYAILFFQTMFQTLVISLALDRKWSQPKLES--WHEYLCPPVGMLSLNLSFMIFSLFDIINTKSLNVFSVELNPYLFMRIFTNGFQSFGVALAILQRLHSSLIFGKMIF 508
BLAST of SMED30034644 vs. Planmine SMEST
Match: SMESG000040908.1 (SMESG000040908.1) HSP 1 Score: 133.265 bits (334), Expect = 2.631e-33 Identity = 151/520 (29.04%), Postives = 263/520 (50.58%), Query Frame = 2 Query: 74 MKSHFENLIFLNIVVTVSLLYFSFGFYFNFSWACWQLDYKSNETEKYM----KIVILGMISCNIINAVIVIGFFIWTIRKMKPNFRNPAN-NFLFYSLSIPLFIVSIVFLMLIPTN---------------CLMEL---YNNXXXXXXXXXXXXXXXXKNLTGFNEINDICQNSVVFITLIGIINHFFFHLIAFHTYSFIEMPPNISLYSQIG-YSVKQYDNFLLSNRVLFEGFLFLFCFVCIAHL---------------KSHKFINKSWSISNSQMVSFSQANEENQNFVFTSKVPSLAILSAIVYLIVTLLAIVIVHV-SHDFGFRGDSCILSTIFPVPAYLFSIYLMILQMKEMAIWRKRKKDEMSIDIKIMWIGVFGQFLVIFINI-FNVHTDEAISSIQLIEYCIMLFQIIFQTVVIGVTLNLEWQPAKKESKLW-ELLHPPVGLLSMNLSFFLFTIYILLTNLCTNPAAKSSMGNNLIKL---GINSISQPLAILHRLHSSFIFGKLLYHTSQ 1498 M+++++ I I+ L+YF G ++SWA +LDYK+ + + I+G+++ + +I+IGFFI+ K K ++ N LFY LSIPL +VS+ +L+L+ C EL I+ I ++ S L+I K + F + IT+IG++N+FFFH+++ Y+FI + N S+Y +I ++ S R + + LFC +C+ +L ++ ++I S+ N Q +S + N N++F+ ++P ++++ I+ + ++L I+I++ +D I+S F YL SI L+ILQ+ EM WRK +++ +D + WIGV GQ ++ I N++ E S+ LIEY ++ QI+ Q V VTL L+ Q + K W E + P VG+LS+NL +++ ++ N + + S + I++ G S + L+IL RLHS IFGK++ +Q Sbjct: 1 METNYKFDILEKILEFSCLIYFVLGCLTSYSWASKELDYKTLNSRILLFWEPIAAIMGIVN---VFHIILIGFFIYKNSKKKIMKKSHKEINILFYILSIPLLLVSMSYLILLIIRDFHCQETIGKFSIFLCFGELGFFIATIVFITFINISKLIIPTKQMAQFFRF-------LKLITIIGMVNYFFFHIVSQQIYNFISVASNTSIYPKINNRNIPALPFDFDSLRNYMQPLVILFCCICVIYLVIIFRLANANNPSISENDEYIQLIESVHNQQNLSQTSKNS-NKSFMKNLQLPPISLILGILLITSSILIIIIINSFLNDKLLYKSGQIISATFASFIYLISIVLLILQIFEMKRWRKNEENFNIVDKILTWIGVCGQLMISTAQISLNIYKFEPASAFLLIEYFFLIVQILLQYVT--VTLALDRQCIPPKIKTWQEFICPAVGILSINLGLLFLSLFDVI-NANSQKSFNSYETDMFIQIFGAGFQSFGETLSILQRLHSLLIFGKIIIIINQ 506
BLAST of SMED30034644 vs. Planmine SMEST
Match: SMESG000040922.1 (SMESG000040922.1) HSP 1 Score: 116.701 bits (291), Expect = 1.196e-27 Identity = 141/507 (27.81%), Postives = 240/507 (47.34%), Query Frame = 2 Query: 74 MKSHFENLIFLNIVVTVSLLYFSFGFYFNFSWACWQLDYKSNETEKYM---KIVILGMISCNIINAVIVIGFFIWTIRKMKPNFRNPANNFLFYSLSIPLFIVSIVFLMLIPTNCLMELYNNXXXXXXXXXXXXXXX--------XKNLTGFN-EINDICQNSVVFI---TLIGIINHFFFHLIAFHTYSFIEMPPNISLYSQI-GYSV--KQYDNFLLSN-RVLFEGFLFLFCFVCIAHLKSHKFINKSWSI-----SNSQ-MVSFSQANEENQNFVFTSKVPSLAILSAIVYLIVT------LLAIVIVHVSHDFGFRGDSCILSTIFPVPAYLFSIYLMILQMKEMAIWRKRKKDEMSIDIKIMWIGVFGQFLVIFINIFN--VHTDEAISSIQLIEYCIMLFQIIFQTVVIGVTLNLEWQPAKKESKLW-ELLHPPVGLLSMNLSFFLFTIYILLTNLCTNPAAKSSMGNNLIKLGINSISQP---LAILHRLHSSFIFGKLL 1483 M + + +F I+ +L YF G+ N+ WAC +LDY S+ E + I ++ IS II +I+I + R PN L Y+L +P F+ ++ F M I ++ Y N + + S +I+ K ++ N E+N Q V FI LIG N + A +F+++ N S Y Q G S+ + D + LSN LFC +C+ L + NKS + ++SQ +V ++ +N +V SL + ++ L + L I+I + + + ++ST+FP+P Y +I L+ +Q+ E+ W+K +E ++D ++WIG GQ ++ F +F + E S++ + +Y ++L QI QTV + + LN +W K +S W E L PP+ LLS+N+ F ++ ++ K+ +++ N P LAI HR+HSS IFGK++ Sbjct: 1 MNESYWSDLFEQILNFFALSYFILGYLTNYLWACNRLDYTSSYKEIFKFWNPIAVITGISNIIIGILIIIALNVRINRVSIPNHFEYQT--LIYTLLVPSFLGTVTF-MAIRIILTIKSYMNFNDFTMVLSFSEMILFCCGIGLIRKKISMVNPEVNCYLQKLVEFINKSALIGFCNCLIHFMSAQQIDNFVKVASNFSFYFQFYGKSLTNRTVDYYNLSNINSYLVPIAILFCCLCVICLFFNLLNNKSQNTLTEDQNDSQVIVQWTSTTNLLENEPIFDRVRSLNMSPLLLILALIFLAISIFLTIIINSFLNSYQYNITGQLISTVFPIPFYFLAIILLAIQINEIMEWKKISTNEFTVDKMMLWIGAAGQLVICFAKLFMEFIERTENFSALIIAKYGLLLVQIFIQTVALNMALNRKWLRPKLKS--WQEKLFPPISLLSLNMGFLFISMLNDFNRNTSHSTVKNIDKRLFLRIFGNGFQLPGLILAIFHRMHSSVIFGKMI 502 The following BLAST results are available for this feature:
BLAST of SMED30034644 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30034644 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30034644 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30034644 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30034644 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30034644 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30034644 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30034644 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30034644 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30034644 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30034644 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30034644 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30034644 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30034644 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30034644 ID=SMED30034644|Name=SMED30034644|organism=Schmidtea mediterranea sexual|type=transcript|length=1588bpback to top protein sequence of SMED30034644-orf-1 >SMED30034644-orf-1 ID=SMED30034644-orf-1|Name=SMED30034644-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=479bp MKSHFENLIFLNIVVTVSLLYFSFGFYFNFSWACWQLDYKSNETEKYMKIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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