Slit-2
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30034592 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of Slit-2 vs. Ensembl Human
Match: LRRC24 (leucine rich repeat containing 24 [Source:HGNC Symbol;Acc:HGNC:28947]) HSP 1 Score: 83.9593 bits (206), Expect = 1.736e-16 Identity = 93/303 (30.69%), Postives = 136/303 (44.88%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLS-LDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKE----VSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELR---CTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGN--NSGRLSCEAINALETNVKWRHKLFASENYYRFQ 1145
GCP+ C+ C S + C +L +PL IP + + N L AL+ LR L L ++ +E AF L L L++NRL+ L SG F GL+ L L L N+ LL+ +F L L +L+ S E++++ L L LS NQL I ++ L+++ L ENP+ C C L WL +K ++ L S R C P L+ Q+L+ +S S+LIC PP V + L N R++C+A + V WR E R Q
Sbjct: 22 GCPAACR---CYSATVECGA-LRLRVVPLGIPPGTQTLFLQDNNI--ARLEPGALAPLA----ALRRLYLHNNSLRALEAGAFRAQPRLLELALTSNRLRGLRSGAFVGLAQLRVLYLAGNQLARLLDFTF-------LHLPELHLQENSIELLEDQALAGLSSLALLDLSRNQLGTISREALQPLASLQVLRLTENPWRCDCALHWLGAWIK---EGGQRLLTSRDRKIMCAEPPRLALQSLLDVSHSSLICIPPSVHVQPLELTANLGEDLRVACQASGYPQPLVTWRKVPQPREGRPRAQ 304
BLAST of Slit-2 vs. Ensembl Human
Match: LRRC24 (leucine rich repeat containing 24 [Source:HGNC Symbol;Acc:HGNC:28947]) HSP 1 Score: 83.1889 bits (204), Expect = 3.436e-16 Identity = 93/303 (30.69%), Postives = 137/303 (45.21%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLS-LDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKE----VSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELR---CTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGN--NSGRLSCEAINALETNVKWRHKLFASENYYRFQ 1145
GCP+ C+ C S + C +L +PL IP + + N L AL+ LR L L ++ +E AF L L L++NRL+ L SG F GL+ L L L N+ LL+ +F L L+ +L+ S E++++ L L LS NQL I ++ L+++ L ENP+ C C L WL +K ++ L S R C P L+ Q+L+ +S S+LIC PP V + L N R++C+A + V WR E R Q
Sbjct: 22 GCPAACR---CYSATVECGA-LRLRVVPLGIPPGTQTLFLQDNNI--ARLEPGALAPLA----ALRRLYLHNNSLRALEAGAFRAQPRLLELALTSNRLRGLRSGAFVGLAQLRVLYLAGNQLARLLDFTFLHLP----RLQELHLQENSIELLEDQALAGLSSLALLDLSRNQLGTISREALQPLASLQVLRLTENPWRCDCALHWLGAWIK---EGGQRLLTSRDRKIMCAEPPRLALQSLLDVSHSSLICIPPSVHVQPLELTANLGEDLRVACQASGYPQPLVTWRKVPQPREGRPRAQ 307
BLAST of Slit-2 vs. Ensembl Human
Match: SLIT2 (slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGNC:11086]) HSP 1 Score: 68.9366 bits (167), Expect = 2.204e-11 Identity = 72/258 (27.91%), Postives = 116/258 (44.96%), Query Frame = 3
Query: 252 VEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLK----SCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
V P+A CP+ C SG V L ++P NIP+ + ++G N T + L H LR+LQL I IE AF L L+ L L+ N L++ +F G + Y RLD +EN I + +FRG V + +L L SC +++ ++ +++ L L L++N ++ + +L L N +C C L WL+ L+ R L ++ C P L G + + + +C
Sbjct: 23 VAPQA-CPAQCS----CSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRH-------LRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLY-RLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISC-IEDGAFRALRD----LEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ--CMGPSHLRGHNVAEVQKREFVC 257 HSP 2 Score: 57.3806 bits (137), Expect = 6.981e-8 Identity = 71/273 (26.01%), Postives = 107/273 (39.19%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLE-GSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKP------PGVKEIDIFLHGNNSGRLSC 1067
CP+ C S +V LT +P N+P+ I I + QN I + A S Y LR + L+ I + P+AF L +L SL L N++ L +F+GL L L N + L +F+ L +L L L N+L I K ++ + L +NPF C C L+WL L A RCT+P+ L+ + + + C PG ++ L G+ L+C
Sbjct: 277 CPAACT----CSNNIVDCRGKGLTEIPTNLPETITEIRLE-QNTIKV-IPPGAFSPYK----KLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDL------------------------HNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGA-----RCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLAC 510 HSP 3 Score: 50.8322 bits (120), Expect = 7.604e-6 Identity = 60/228 (26.32%), Postives = 93/228 (40.79%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNP 947
CP C+ + G V KL +P +IP+ + ++ F T L + P+ LR + + I +IE AF + + L++NRL+ + +FKGL SL L L +N + SF GL SS+ L L L NQ++ + L ++L NPF C C L WL + L+ K++ + RC P
Sbjct: 509 ACPEKCRCE----GTTVDCSNQKLNKIPEHIPQYTAELRLNNNEF--TVLEATGIFKKLPQ---LRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGL--SSVRL----------------------LSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLR-----KKRIVTGNPRCQKP 698
BLAST of Slit-2 vs. Ensembl Human
Match: SLIT2 (slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGNC:11086]) HSP 1 Score: 68.9366 bits (167), Expect = 2.219e-11 Identity = 72/258 (27.91%), Postives = 116/258 (44.96%), Query Frame = 3
Query: 252 VEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLK----SCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
V P+A CP+ C SG V L ++P NIP+ + ++G N T + L H LR+LQL I IE AF L L+ L L+ N L++ +F G + Y RLD +EN I + +FRG V + +L L SC +++ ++ +++ L L L++N ++ + +L L N +C C L WL+ L+ R L ++ C P L G + + + +C
Sbjct: 23 VAPQA-CPAQCS----CSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRH-------LRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLY-RLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISC-IEDGAFRALRD----LEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ--CMGPSHLRGHNVAEVQKREFVC 257 HSP 2 Score: 57.3806 bits (137), Expect = 6.853e-8 Identity = 71/273 (26.01%), Postives = 107/273 (39.19%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLE-GSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKP------PGVKEIDIFLHGNNSGRLSC 1067
CP+ C S +V LT +P N+P+ I I + QN I + A S Y LR + L+ I + P+AF L +L SL L N++ L +F+GL L L N + L +F+ L +L L L N+L I K ++ + L +NPF C C L+WL L A RCT+P+ L+ + + + C PG ++ L G+ L+C
Sbjct: 273 CPAACT----CSNNIVDCRGKGLTEIPTNLPETITEIRLE-QNTIKV-IPPGAFSPYK----KLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDL------------------------HNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGA-----RCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLAC 506 HSP 3 Score: 50.8322 bits (120), Expect = 7.340e-6 Identity = 60/228 (26.32%), Postives = 93/228 (40.79%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNP 947
CP C+ + G V KL +P +IP+ + ++ F T L + P+ LR + + I +IE AF + + L++NRL+ + +FKGL SL L L +N + SF GL SS+ L L L NQ++ + L ++L NPF C C L WL + L+ K++ + RC P
Sbjct: 505 ACPEKCRCE----GTTVDCSNQKLNKIPEHIPQYTAELRLNNNEF--TVLEATGIFKKLPQ---LRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGL--SSVRL----------------------LSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLR-----KKRIVTGNPRCQKP 694
BLAST of Slit-2 vs. Ensembl Human
Match: SLIT2 (slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGNC:11086]) HSP 1 Score: 68.5514 bits (166), Expect = 2.459e-11 Identity = 72/258 (27.91%), Postives = 116/258 (44.96%), Query Frame = 3
Query: 252 VEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLK----SCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
V P+A CP+ C SG V L ++P NIP+ + ++G N T + L H LR+LQL I IE AF L L+ L L+ N L++ +F G + Y RLD +EN I + +FRG V + +L L SC +++ ++ +++ L L L++N ++ + +L L N +C C L WL+ L+ R L ++ C P L G + + + +C
Sbjct: 23 VAPQA-CPAQCS----CSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRH-------LRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLY-RLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISC-IEDGAFRALRD----LEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ--CMGPSHLRGHNVAEVQKREFVC 257 HSP 2 Score: 56.225 bits (134), Expect = 1.625e-7 Identity = 70/267 (26.22%), Postives = 106/267 (39.70%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLE-GSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSC 1067
CP+ C S +V LT +P N+P+ I I + QN I + A S Y LR + L+ I + P+AF L +L SL L N++ L +F+GL L L N + L +F+ L +L L L N+L I K ++ + L +NPF C C L+WL L A RCT+P+ L+ + + + C G ++ L G+ L+C
Sbjct: 277 CPAACT----CSNNIVDCRGKGLTEIPTNLPETITEIRLE-QNTIKV-IPPGAFSPYK----KLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDL------------------------HNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGA-----RCTSPRRLANKRIGQIKSKKFRCS--GTEDYRSKLSGDCFADLAC 502 HSP 3 Score: 50.8322 bits (120), Expect = 8.556e-6 Identity = 60/228 (26.32%), Postives = 93/228 (40.79%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNP 947
CP C+ + G V KL +P +IP+ + ++ F T L + P+ LR + + I +IE AF + + L++NRL+ + +FKGL SL L L +N + SF GL SS+ L L L NQ++ + L ++L NPF C C L WL + L+ K++ + RC P
Sbjct: 501 ACPEKCRCE----GTTVDCSNQKLNKIPEHIPQYTAELRLNNNEF--TVLEATGIFKKLPQ---LRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGL--SSVRL----------------------LSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLR-----KKRIVTGNPRCQKP 690
BLAST of Slit-2 vs. Ensembl Celegans
Match: slt-1 (Slit homolog 1 protein [Source:UniProtKB/Swiss-Prot;Acc:G5EFX6]) HSP 1 Score: 62.7734 bits (151), Expect = 5.696e-10 Identity = 70/252 (27.78%), Postives = 115/252 (45.63%), Query Frame = 3
Query: 264 AGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQ----INLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFK-GLSLDYLRLDNNENLILLEGSFRG-LVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
A CP+ C C+ + C+ +LT +P NIP + I L N ++ GP +NL+ LQL I I ++FS L+ L+ L LS NR++ L +F+ L L +L L N+ ++ + +G + L+L ++ L +I L L L+ N+L+ E R +DL NP+ C C+LRW+ K L+ + + K + C P L G ++ L + + C
Sbjct: 15 ATCPAECV---CVDRTVSCVG-QQLTEVPQNIP----------NDTIRLDLQDNEITKIGPNDFSSLMNLKALQLMDNQIVTIHNQSFSSLVFLQKLRLSRNRIRHLPDNVFQNNLKLTHLDLSENDITVVSDAQLQGPEFLEVLNLDKNHIFCLENNVISSWVS-LEVLTLNGNRLTTF---EEPSNARFRQLDLFNNPWNCDCRLRWMRKWLE-KAEGQNKTV-----CATPLNLQGSSIEILQDKFMTC 242
BLAST of Slit-2 vs. Ensembl Celegans
Match: pxn-1 (Peroxidasin homolog [Source:UniProtKB/Swiss-Prot;Acc:Q1ENI8]) HSP 1 Score: 52.7582 bits (125), Expect = 7.166e-7 Identity = 62/266 (23.31%), Postives = 116/266 (43.61%), Query Frame = 3
Query: 456 NLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELR------CTNPKELSGQTLISLSESNLICKPPG-----VKEIDIFLHGNNSGRLSCEAINALETNVKWRHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSC 1217
N+R L + I ++ L++L L++N++KI+ + L L L L +NE + + ++SL+LK +++ + ++++ LT+L SHN++ + K L L NP+ C C+ +K KAL+ +++ CTNP EL Q L + +S L C P +EI + N+S +L +E + +S + Y D ++I++ + NV+ +C
Sbjct: 53 NVRSLVIRNNRIHKLKRSDLEGFNQLETLVLTHNKIKIIEENVLDHLPELKRLSLAHNELVYIPPLCSDSRPLASLNLKRNHIQFIDEQVLRYFPD-LTQLDFSHNRIQSLRTKLFDNLPALSHAHLHSNPWHCDCRA------------SKVKALLQKVKWEKKVYCTNPVELRHQALDEVDDSALTCARPAEESWTGEEIKLTCAKNSSSKLVVWMYENVEVDS-------SSLDGYEIHD-------TVITVPRKTNVNFMTC 291
BLAST of Slit-2 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330730) HSP 1 Score: 85.5001 bits (210), Expect = 6.863e-17 Identity = 75/248 (30.24%), Postives = 110/248 (44.35%), Query Frame = 3
Query: 264 AGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLV-VSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
A CP C +G V LT++P I + + + G N T+ Y LR+LQLT I IE +F L++L+ LD+SNN + + +FKG SL L+LDNN+ L E +F+GLV + L+L + L +L + I + G L L LS +NPF C C L WL++ L++ L RC +P +L GQ + L + C
Sbjct: 71 ARCPRVCS----CTGLNVDCSHRGLTSVPRKISADVERLELQGNNL---TVIYETDFQ---RLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGL-GRLRALRLS------------------------DNPFACDCHLSWLSRFLRSATR-----LAPYTRCQSPSQLKGQNVADLHDQEFKC 278 HSP 2 Score: 60.4622 bits (145), Expect = 3.905e-9 Identity = 76/252 (30.16%), Postives = 108/252 (42.86%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDN-NENLILLEGSFRGLVVSSL------SLKSCYLKNLSYEIIKEVSG-HLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
CP C+ C G + C E LT++P+ +P + + QNFI T L + S + LR + L+ NI I +A S L L +L L N++K L SG+FKGL L L N NE + + +FR L SL +++S L N +++ +K + HL K NPF C C LRWL L K S RC +PK + + + SL E C
Sbjct: 295 CPHPCR---CADGIVDCRE-KSLTSVPVTLPDDTTELRLE-QNFI-TELPPKSFSSF----RRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQS--LANGTFDAMKSIKTVHLAK-----------------------------NPFICDCNLRWLADYLH-----KNPIETSGARCESPKRMHRRRIESLREEKFKC 500
BLAST of Slit-2 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330729) HSP 1 Score: 85.5001 bits (210), Expect = 7.500e-17 Identity = 75/248 (30.24%), Postives = 110/248 (44.35%), Query Frame = 3
Query: 264 AGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLV-VSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
A CP C +G V LT++P I + + + G N T+ Y LR+LQLT I IE +F L++L+ LD+SNN + + +FKG SL L+LDNN+ L E +F+GLV + L+L + L +L + I + G L L LS +NPF C C L WL++ L++ L RC +P +L GQ + L + C
Sbjct: 71 ARCPRVCS----CTGLNVDCSHRGLTSVPRKISADVERLELQGNNL---TVIYETDFQ---RLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGL-GRLRALRLS------------------------DNPFACDCHLSWLSRFLRSATR-----LAPYTRCQSPSQLKGQNVADLHDQEFKC 278 HSP 2 Score: 60.4622 bits (145), Expect = 4.224e-9 Identity = 76/252 (30.16%), Postives = 108/252 (42.86%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDN-NENLILLEGSFRGLVVSSL------SLKSCYLKNLSYEIIKEVSG-HLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
CP C+ C G + C E LT++P+ +P + + QNFI T L + S + LR + L+ NI I +A S L L +L L N++K L SG+FKGL L L N NE + + +FR L SL +++S L N +++ +K + HL K NPF C C LRWL L K S RC +PK + + + SL E C
Sbjct: 295 CPHPCR---CADGIVDCRE-KSLTSVPVTLPDDTTELRLE-QNFI-TELPPKSFSSF----RRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQS--LANGTFDAMKSIKTVHLAK-----------------------------NPFICDCNLRWLADYLH-----KNPIETSGARCESPKRMHRRRIESLREEKFKC 500
BLAST of Slit-2 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330732) HSP 1 Score: 85.5001 bits (210), Expect = 7.500e-17 Identity = 75/248 (30.24%), Postives = 110/248 (44.35%), Query Frame = 3
Query: 264 AGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLV-VSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
A CP C +G V LT++P I + + + G N T+ Y LR+LQLT I IE +F L++L+ LD+SNN + + +FKG SL L+LDNN+ L E +F+GLV + L+L + L +L + I + G L L LS +NPF C C L WL++ L++ L RC +P +L GQ + L + C
Sbjct: 71 ARCPRVCS----CTGLNVDCSHRGLTSVPRKISADVERLELQGNNL---TVIYETDFQ---RLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGL-GRLRALRLS------------------------DNPFACDCHLSWLSRFLRSATR-----LAPYTRCQSPSQLKGQNVADLHDQEFKC 278 HSP 2 Score: 60.4622 bits (145), Expect = 4.224e-9 Identity = 76/252 (30.16%), Postives = 108/252 (42.86%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDN-NENLILLEGSFRGLVVSSL------SLKSCYLKNLSYEIIKEVSG-HLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
CP C+ C G + C E LT++P+ +P + + QNFI T L + S + LR + L+ NI I +A S L L +L L N++K L SG+FKGL L L N NE + + +FR L SL +++S L N +++ +K + HL K NPF C C LRWL L K S RC +PK + + + SL E C
Sbjct: 295 CPHPCR---CADGIVDCRE-KSLTSVPVTLPDDTTELRLE-QNFI-TELPPKSFSSF----RRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQS--LANGTFDAMKSIKTVHLAK-----------------------------NPFICDCNLRWLADYLH-----KNPIETSGARCESPKRMHRRRIESLREEKFKC 500
BLAST of Slit-2 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330734) HSP 1 Score: 77.0258 bits (188), Expect = 2.897e-14 Identity = 75/250 (30.00%), Postives = 110/250 (44.00%), Query Frame = 3
Query: 264 AGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVS----GHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
A CP C +G V LT++P I + + + G N T+ Y LR+LQLT I IE +F L++L+ L L+NNRLK + F S LRLD + N+I G R + + SL+S L N + E + L L L++N L+ + RL + L +NPF C C L WL++ L++ L RC +P +L GQ + L + C
Sbjct: 71 ARCPRVCS----CTGLNVDCSHRGLTSVPRKISADVERLELQGNNL---TVIYETDFQ---RLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPEN-FVTSSASLLRLDISNNVITTVG--RRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATR-----LAPYTRCQSPSQLKGQNVADLHDQEFKC 302 HSP 2 Score: 62.3882 bits (150), Expect = 1.028e-9 Identity = 76/252 (30.16%), Postives = 108/252 (42.86%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDN-NENLILLEGSFRGLVVSSL------SLKSCYLKNLSYEIIKEVSG-HLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
CP C+ C G + C E LT++P+ +P + + QNFI T L + S + LR + L+ NI I +A S L L +L L N++K L SG+FKGL L L N NE + + +FR L SL +++S L N +++ +K + HL K NPF C C LRWL L K S RC +PK + + + SL E C
Sbjct: 319 CPHPCR---CADGIVDCRE-KSLTSVPVTLPDDTTELRLE-QNFI-TELPPKSFSSF----RRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQS--LANGTFDAMKSIKTVHLAK-----------------------------NPFICDCNLRWLADYLH-----KNPIETSGARCESPKRMHRRRIESLREEKFKC 524
BLAST of Slit-2 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330733) HSP 1 Score: 76.2554 bits (186), Expect = 5.943e-14 Identity = 75/250 (30.00%), Postives = 110/250 (44.00%), Query Frame = 3
Query: 264 AGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVS----GHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
A CP C +G V LT++P I + + + G N T+ Y LR+LQLT I IE +F L++L+ L L+NNRLK + F S LRLD + N+I G R + + SL+S L N + E + L L L++N L+ + RL + L +NPF C C L WL++ L++ L RC +P +L GQ + L + C
Sbjct: 71 ARCPRVCS----CTGLNVDCSHRGLTSVPRKISADVERLELQGNNL---TVIYETDFQ---RLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPEN-FVTSSASLLRLDISNNVITTVG--RRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATR-----LAPYTRCQSPSQLKGQNVADLHDQEFKC 302 HSP 2 Score: 60.4622 bits (145), Expect = 3.956e-9 Identity = 76/252 (30.16%), Postives = 108/252 (42.86%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDN-NENLILLEGSFRGLVVSSL------SLKSCYLKNLSYEIIKEVSG-HLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
CP C+ C G + C E LT++P+ +P + + QNFI T L + S + LR + L+ NI I +A S L L +L L N++K L SG+FKGL L L N NE + + +FR L SL +++S L N +++ +K + HL K NPF C C LRWL L K S RC +PK + + + SL E C
Sbjct: 319 CPHPCR---CADGIVDCRE-KSLTSVPVTLPDDTTELRLE-QNFI-TELPPKSFSSF----RRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQS--LANGTFDAMKSIKTVHLAK-----------------------------NPFICDCNLRWLADYLH-----KNPIETSGARCESPKRMHRRRIESLREEKFKC 524
BLAST of Slit-2 vs. Ensembl Zebrafish
Match: si:dkey-1j5.4 (si:dkey-1j5.4 [Source:ZFIN;Acc:ZDB-GENE-130603-91]) HSP 1 Score: 65.855 bits (159), Expect = 3.778e-11 Identity = 63/213 (29.58%), Postives = 100/213 (46.95%), Query Frame = 3
Query: 384 ISGQNFINTTLSYNALSHYGPEQ----INLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLS-LDYLRLDNNENLILLEGSFRGLV-VSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICK 1004
I +N L NAL+ P I L++L L+ I+ I P+AF L L L++ NN+LK + +G F L+ L+ L LDNN L + GL + L ++ +++L ++ + LT + LS N+L ID + L+ + L +NP+ C C + L L Q + LRC +P+E + + L L CK
Sbjct: 147 IGMRNLSELDLPLNALTALPPNAFKPLIALKVLDLSLNRIQRISPKAFVGLEELLFLNMDNNKLKSIQAGTFGPLTGLEMLVLDNNLLSTLTVSTLEGLSNLQELYVRKNEIESLPADVFLH-TPKLTHVGLSGNRLHAIDGNMLANMQGLKEVFLHDNPWKCDCSINSLVHYLA--QTKTNHSPRQRLRCASPEEFRDRAIQELKSEELSCK 356
BLAST of Slit-2 vs. Ensembl Zebrafish
Match: si:cabz01090165.1 (si:cabz01090165.1 [Source:ZFIN;Acc:ZDB-GENE-161017-71]) HSP 1 Score: 66.6254 bits (161), Expect = 4.528e-11 Identity = 87/374 (23.26%), Postives = 148/374 (39.57%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLV--VSSLSLKSCYLKNLSYEIIKEVSG-----------------------HLTKLYLSHNQLSGIX-----------XXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKE--IDIFLHGNNSGRLSCEAINALETNVKW---RHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNIM 1265
CP C Q L V L +P NI + + + NFI T+L + ++ +L L L+ I I P AF+ L L +L L NRL +L +GL +L +L L NN+ + EG+F+ + + L L L +L ++ I ++ L +L ++ N+L I K + ++ NP C C+L WL R+ +E L + C +P+EL+G+ ++ E +C+PP + +F+ L C ++ E V W KL A+ + D G I + + ++C N+ G+ V ++
Sbjct: 23 CPKRCTCQNLLPSYTVLCAKTGLLFVPPNIDRQTAELRLM-DNFI-TSLRHRDFANMS----SLIHLTLSRNTISQIRPYAFADLQDLHALHLDANRLTVLDDTHLQGLVNLRHLILANNQIHSISEGAFQDFLETLEDLDLSYNNLVDLPWDTIAMLASVNTLSLDHNLIEFVPEGIFSNLHKLARLDMTSNKLKKIPPDPLFLRIPVYAKMKGSPLTALVLSFGGNPLHCNCELVWL------RRLTREDDLET---CASPRELAGKYFWTIREEEFVCEPPMITRHTSKMFVMEGQEVSLRCRSVGDPEPIVHWISPDGKLIANTSRTVCYDN----GSLDILTATVKDSGVFTCIASNAAGEATAPVELV 377
BLAST of Slit-2 vs. Ensembl Zebrafish
Match: si:cabz01090165.1 (si:cabz01090165.1 [Source:ZFIN;Acc:ZDB-GENE-161017-71]) HSP 1 Score: 66.6254 bits (161), Expect = 4.528e-11 Identity = 87/374 (23.26%), Postives = 148/374 (39.57%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLV--VSSLSLKSCYLKNLSYEIIKEVSG-----------------------HLTKLYLSHNQLSGIX-----------XXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKE--IDIFLHGNNSGRLSCEAINALETNVKW---RHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNIM 1265
CP C Q L V L +P NI + + + NFI T+L + ++ +L L L+ I I P AF+ L L +L L NRL +L +GL +L +L L NN+ + EG+F+ + + L L L +L ++ I ++ L +L ++ N+L I K + ++ NP C C+L WL R+ +E L + C +P+EL+G+ ++ E +C+PP + +F+ L C ++ E V W KL A+ + D G I + + ++C N+ G+ V ++
Sbjct: 23 CPKRCTCQNLLPSYTVLCAKTGLLFVPPNIDRQTAELRLM-DNFI-TSLRHRDFANMS----SLIHLTLSRNTISQIRPYAFADLQDLHALHLDANRLTVLDDTHLQGLVNLRHLILANNQIHSISEGAFQDFLETLEDLDLSYNNLVDLPWDTIAMLASVNTLSLDHNLIEFVPEGIFSNLHKLARLDMTSNKLKKIPPDPLFLRIPVYAKMKGSPLTALVLSFGGNPLHCNCELVWL------RRLTREDDLET---CASPRELAGKYFWTIREEEFVCEPPMITRHTSKMFVMEGQEVSLRCRSVGDPEPIVHWISPDGKLIANTSRTVCYDN----GSLDILTATVKDSGVFTCIASNAAGEATAPVELV 377
BLAST of Slit-2 vs. Ensembl Zebrafish
Match: slit2 (slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-010306-3]) HSP 1 Score: 65.4698 bits (158), Expect = 1.631e-10 Identity = 70/262 (26.72%), Postives = 112/262 (42.75%), Query Frame = 3
Query: 249 CVEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLK----SCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPP 1010
C CPS C SG V L ++P NIP+ + + ++ N T + L NLR+LQL I +IE AF L L+ L L+ N L++L +F G + RLD +EN I + +FRG + +L L SC +++ ++ +++ L L L++N +S + +L L N C C + WL+ L+ R L +C P L G + + + +C P
Sbjct: 14 CGAGAQSCPSQCS----CSGTAVDCHGQSLRSVPRNIPRNVERLDLNANNLTKITKADFAGLK-------NLRVLQLMENKISSIERGAFQDLQELERLRLNRNNLQVLPELLFLG-TTKLFRLDLSENQIQGIPRKAFRGSTEIKNLQLDYNQISC-IEDGAFRALRD----LEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLLCDCNVAWLSDWLRQRPR-----LGLYTQCMAPPSLRGHNIAEVQKKEFMCTGP 253 HSP 2 Score: 56.6102 bits (135), Expect = 7.498e-8 Identity = 73/268 (27.24%), Postives = 113/268 (42.16%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKN-LSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSC 1067
CP C S +V LT +P N+P+ I I + EQ +++I I AF+ L+ +DLSNN++ L S F+GL SL+ L L N+ L +G F GL L L + N L + +++ +L L L N+L I K ++ + L +NPF C C L+WL L+ A RCT+P+ L+ + + + C GV++ L G+ L+C
Sbjct: 264 CPELCT----CSNNVVDCRGKGLTEIPTNLPETITEIRL--------------------EQNSIKI----------IPAGAFAPYKRLRRIDLSNNQITELASDSFQGLRSLNSLVLYGNKITELPKGLFDGLFSLQLLLLNANKINCLRVDSFQDLQ-NLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFMCDCHLKWLADYLQDNPIETSGA-----RCTSPRRLANKRIGQIKSKKFRCS--GVEDYRSKLGGDCFADLAC 489 HSP 3 Score: 54.299 bits (129), Expect = 4.917e-7 Identity = 65/228 (28.51%), Postives = 96/228 (42.11%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLS-LDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNP 947
CP C+ + G V KLT +P +IP+ + ++ F T L + P+ LR + L+ I +IE F + L L++NRL+ +H + KGL L L L +N + GSF GL SS+ L S Y NL I G L+ LS ++ NPF C C L WL + L+ K++ + RC +P
Sbjct: 488 ACPEKCRCE----GTTVDCSGQKLTKIPDHIPQYTAELRLNNNEF--TVLEATGIFKKLPQ---LRKINLSNNKITDIEEGTFEGASGVNELILTSNRLEGVHYSMLKGLGGLRTLMLRSNRISCVNNGSFTGL--SSVRLLSLY-DNL---ITSMSPGAFDTLH----SLSTLNLLA--------------NPFNCNCHLAWLGEWLR-----KKRIVTGNPRCQSP 677
BLAST of Slit-2 vs. Ensembl Zebrafish
Match: slit2 (slit homolog 2 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-010306-3]) HSP 1 Score: 65.0846 bits (157), Expect = 1.806e-10 Identity = 70/262 (26.72%), Postives = 112/262 (42.75%), Query Frame = 3
Query: 249 CVEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLK----SCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPP 1010
C CPS C SG V L ++P NIP+ + + ++ N T + L NLR+LQL I +IE AF L L+ L L+ N L++L +F G + RLD +EN I + +FRG + +L L SC +++ ++ +++ L L L++N +S + +L L N C C + WL+ L+ R L +C P L G + + + +C P
Sbjct: 14 CGAGAQSCPSQCS----CSGTAVDCHGQSLRSVPRNIPRNVERLDLNANNLTKITKADFAGLK-------NLRVLQLMENKISSIERGAFQDLQELERLRLNRNNLQVLPELLFLG-TTKLFRLDLSENQIQGIPRKAFRGSTEIKNLQLDYNQISC-IEDGAFRALRD----LEVLTLNNNNISRLSVASFNHMPKLRTFRLHSNNLLCDCNVAWLSDWLRQRPR-----LGLYTQCMAPPSLRGHNIAEVQKKEFMCTGP 253 HSP 2 Score: 56.6102 bits (135), Expect = 8.299e-8 Identity = 73/268 (27.24%), Postives = 113/268 (42.16%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKN-LSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSC 1067
CP C S +V LT +P N+P+ I I + EQ +++I I AF+ L+ +DLSNN++ L S F+GL SL+ L L N+ L +G F GL L L + N L + +++ +L L L N+L I K ++ + L +NPF C C L+WL L+ A RCT+P+ L+ + + + C GV++ L G+ L+C
Sbjct: 264 CPELCT----CSNNVVDCRGKGLTEIPTNLPETITEIRL--------------------EQNSIKI----------IPAGAFAPYKRLRRIDLSNNQITELASDSFQGLRSLNSLVLYGNKITELPKGLFDGLFSLQLLLLNANKINCLRVDSFQDLQ-NLNLLSLYDNKLQTIAKGTFSSLRAIQTLHLAQNPFMCDCHLKWLADYLQDNPIETSGA-----RCTSPRRLANKRIGQIKSKKFRCS--GVEDYRSKLGGDCFADLAC 489 HSP 3 Score: 53.9138 bits (128), Expect = 5.215e-7 Identity = 65/228 (28.51%), Postives = 96/228 (42.11%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLS-LDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNP 947
CP C+ + G V KLT +P +IP+ + ++ F T L + P+ LR + L+ I +IE F + L L++NRL+ +H + KGL L L L +N + GSF GL SS+ L S Y NL I G L+ LS ++ NPF C C L WL + L+ K++ + RC +P
Sbjct: 488 ACPEKCRCE----GTTVDCSGQKLTKIPDHIPQYTAELRLNNNEF--TVLEATGIFKKLPQ---LRKINLSNNKITDIEEGTFEGASGVNELILTSNRLEGVHYSMLKGLGGLRTLMLRSNRISCVNNGSFTGL--SSVRLLSLY-DNL---ITSMSPGAFDTLH----SLSTLNLLA--------------NPFNCNCHLAWLGEWLR-----KKRIVTGNPRCQSP 677
BLAST of Slit-2 vs. Ensembl Xenopus
Match: ENSXETT00000007893.1 (leucine rich repeat and fibronectin type III domain containing 4 [Source:NCBI gene;Acc:101734445]) HSP 1 Score: 70.0922 bits (170), Expect = 5.810e-12 Identity = 92/374 (24.60%), Postives = 156/374 (41.71%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNK-LTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKG-LSLDYLRLDNNENLILLEGSFRG--LVVSSLSLK----------------SCYLKNLSYEIIKEV-SGHLTKLY------LSHNQL-----------SGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKE--IDIFLHGNNSGRLSCEAINALETNVKW---RHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNI 1262
CP +C Q LS L + NK L +P I + + ++ NFI + L+ G L L L+ I+NI+P AF L +L+SL L NRL ++H +G L+L +L ++NN+ + + +F L + L L S + NL + +I+ V G ++LY ++ N+L S + +++ NPF C C+L WL R+ +E + + C +P +L+G+ S+ E C+PP + +++ L C AI E + W K+ N R Y G I + + + Y+C N+ G+ V++
Sbjct: 21 CPFHCTCQN-LSESLSTLCANKGLLYVPPYIDRRTVELRLA-DNFIRVVEQEDFLNMTG-----LVDLTLSRNTIDNIKPYAFGDLESLRSLHLDGNRLTLIHEEALRGMLNLQHLIINNNQLVTIPVATFDDFHLTLEDLDLSYNNLVSVPWEAIQNMVSLHTLNLDHNLIESVMEGTFSELYKLSRLDMTSNRLHTLPPDPLFVRSQTGVISPTPYTSTIVLNFGGNPFHCNCELLWL------RRLVREDDMET---CASPSQLAGRYFWSIPEEEFTCEPPLITRHTHQLWVLEGQRATLKCRAIGDPEPVIHWVSPDDKIMP--NSSRTASYRN--GTLDILVTTIRDDGSYTCIAINAAGESTAQVDL 374
BLAST of Slit-2 vs. Ensembl Xenopus
Match: rrn3 (RRN3 homolog, RNA polymerase I transcription factor [Source:Xenbase;Acc:XB-GENE-990491]) HSP 1 Score: 67.0106 bits (162), Expect = 5.127e-11 Identity = 88/375 (23.47%), Postives = 153/375 (40.80%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNK-LTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLV--VSSLSLKSCYLKNLSYEIIKEVSG-----------------------HLTKLYLSHNQL-----------SGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEID---IFLHGNNSGRLSCEAINALETNVKW---RHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNI 1262
CP YC Q LS L + P K L +P++I + + + G NFI + + + G L L L+ I I+P +F L +L+SL L +NRL L +F+GL +L +L ++NN+ + E +F L+ + L L LK + ++ ++ + L +L L+ N+L S I F + NP C C+L WL R+ +E + + C +P L G+ + E +C+PP + + + L G + L C+A+ + W +L + + D G I I + + ++C N+ G++ V +
Sbjct: 20 CPKYCVCQN-LSESLGTLCPTKGLLFVPMDIDRRTVELRLGG-NFIISINRQDFANMTG-----LVDLTLSRNTISYIQPYSFIDLESLRSLHLDSNRLTNLGDDVFRGLINLQHLIMNNNQLNSIAEDAFEDLLQTLEDLDLSYNNLKTVPWDAVRRMVNLHQLSLDHNLIYYITEGTFQDLNKLARLDLTSNRLQKLPPDPIFARSQISMTSATPFSPPLSLSFGGNPLHCNCELLWL------RRLDREDDMET---CASPSALKGRYFWYVREEEFVCEPPLITQYTQRLLVLEG-QTASLKCKAVGDPLPVIHWVAPDGRLIGNSSRTTVYDN----GTLDILITTSKDYGTFTCIAANAAGELTAPVEL 373
BLAST of Slit-2 vs. Ensembl Xenopus
Match: slit1 (slit guidance ligand 1 [Source:NCBI gene;Acc:100036682]) HSP 1 Score: 67.0106 bits (162), Expect = 7.639e-11 Identity = 74/254 (29.13%), Postives = 113/254 (44.49%), Query Frame = 3
Query: 249 CVEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
CV GCP+ C SG V L +P NIP+ + + G N T ++ N S G +Q LR+LQL I N+E AF + L+ L L+ N+L L +F+ + RLD +ENLI + +FRG + +L L + + + + G L L L++N ++ I +L L N FC C L WL++ L+ R L ++ CT P L G + + + C
Sbjct: 13 CVLCANGCPALCT----CSGTTVDCHGLALKGVPKNIPRSTERLELYGNNI--TRINKNDFS--GLKQ--LRVLQLMENQISNVERGAFDDMKELERLRLNRNQLHTLPELLFQN-NQALSRLDLSENLIQAIPRKTFRGATELKNLQLDKNQISCIEDGAFRALRG-LEVLTLNNNNITAIPVSSFNHMPKLRTFRLHSNNLFCDCHLAWLSQWLRQRPTI---GLFTQ--CTGPSPLRGLNVAEIQKHEFSC 249 HSP 2 Score: 57.7658 bits (138), Expect = 5.218e-8 Identity = 61/209 (29.19%), Postives = 84/209 (40.19%), Query Frame = 3
Query: 333 KLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKEL 956
KLT +P IP+ + ++ TL L P +L+ + L+ I IE AF ++ L L+ N L+ + SG+F+GL L L L NN + SF GL L L L NQ+S I + L ++L NPF C CQL WL L+ R K + RC NP L
Sbjct: 512 KLTKIPDRIPQSTAELRLNNNEI--ATLEATGLFRKLP---HLKKINLSNNKISEIEDAAFEGAASVSELHLTANHLESVRSGMFRGLEGLRTLMLRNNRVSCVHNDSFTGLRNVRL------------------------LSLYDNQISTITPGAFDTLQSLSTLNLLANPFNCNCQLAWLGDWLRKR-----KIVTGNPRCQNPDFL 686 HSP 3 Score: 55.8398 bits (133), Expect = 1.882e-7 Identity = 66/256 (25.78%), Postives = 105/256 (41.02%), Query Frame = 3
Query: 264 AGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEG-SFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC--KPPGVKE 1022
CP+ C C +G + C LTA+P N+P+ + I + ++ A S Y LR + L+ I I P+AF L +L SL L N++ L G+F GL L L N + + +F+ L+NLS L L N++ + K ++ + L +NPF C C L+WL L+ A RC +P+ L+ + + + C K PG ++
Sbjct: 266 GSCPAMCT---CSNGIVDC-RGKGLTAIPANLPETMTEIRLELNGI--KSVPPGAFSPYK----KLRRIDLSNNQISEIAPDAFQGLRSLNSLVLYGNKITDLPKGVFSGLHALQLLLLNANKINCIRADTFQD------------LQNLSL------------LSLYDNKIQSLAKGTFAALRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETSGA-----RCASPRRLANKRIGQIKSKKFRCSGKMPGTED 482
BLAST of Slit-2 vs. Ensembl Xenopus
Match: slit1 (slit guidance ligand 1 [Source:NCBI gene;Acc:100036682]) HSP 1 Score: 66.2402 bits (160), Expect = 1.243e-10 Identity = 72/248 (29.03%), Postives = 111/248 (44.76%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
GCP+ C SG V L +P NIP+ + + G N T ++ N S G +Q LR+LQL I N+E AF + L+ L L+ N+L L +F+ + RLD +ENLI + +FRG + +L L + + + + G L L L++N ++ I +L L N FC C L WL++ L+ R L ++ CT P L G + + + C
Sbjct: 32 GCPALCT----CSGTTVDCHGLALKGVPKNIPRSTERLELYGNNI--TRINKNDFS--GLKQ--LRVLQLMENQISNVERGAFDDMKELERLRLNRNQLHTLPELLFQN-NQALSRLDLSENLIQAIPRKTFRGATELKNLQLDKNQISCIEDGAFRALRG-LEVLTLNNNNITAIPVSSFNHMPKLRTFRLHSNNLFCDCHLAWLSQWLRQRPTI---GLFTQ--CTGPSPLRGLNVAEIQKHEFSC 262 HSP 2 Score: 57.7658 bits (138), Expect = 5.435e-8 Identity = 61/209 (29.19%), Postives = 84/209 (40.19%), Query Frame = 3
Query: 333 KLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKEL 956
KLT +P IP+ + ++ TL L P +L+ + L+ I IE AF ++ L L+ N L+ + SG+F+GL L L L NN + SF GL L L L NQ+S I + L ++L NPF C CQL WL L+ R K + RC NP L
Sbjct: 529 KLTKIPDRIPQSTAELRLNNNEI--ATLEATGLFRKLP---HLKKINLSNNKISEIEDAAFEGAASVSELHLTANHLESVRSGMFRGLEGLRTLMLRNNRVSCVHNDSFTGLRNVRL------------------------LSLYDNQISTITPGAFDTLQSLSTLNLLANPFNCNCQLAWLGDWLRKR-----KIVTGNPRCQNPDFL 703 HSP 3 Score: 54.299 bits (129), Expect = 6.377e-7 Identity = 63/247 (25.51%), Postives = 100/247 (40.49%), Query Frame = 3
Query: 264 AGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEG-SFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
CP+ C C +G + C LTA+P N+P+ + I + ++ A S Y LR + L+ I I P+AF L +L SL L N++ L G+F GL L L N + + +F+ L+NLS L L N++ + K ++ + L +NPF C C L+WL L+ A RC +P+ L+ + + + C
Sbjct: 279 GSCPAMCT---CSNGIVDC-RGKGLTAIPANLPETMTEIRLELNGI--KSVPPGAFSPYK----KLRRIDLSNNQISEIAPDAFQGLRSLNSLVLYGNKITDLPKGVFSGLHALQLLLLNANKINCIRADTFQD------------LQNLSL------------LSLYDNKIQSLAKGTFAALRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETSGA-----RCASPRRLANKRIGQIKSKKFRC 486
BLAST of Slit-2 vs. Ensembl Xenopus
Match: slit1 (slit guidance ligand 1 [Source:NCBI gene;Acc:100036682]) HSP 1 Score: 66.2402 bits (160), Expect = 1.253e-10 Identity = 72/248 (29.03%), Postives = 111/248 (44.76%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
GCP+ C SG V L +P NIP+ + + G N T ++ N S G +Q LR+LQL I N+E AF + L+ L L+ N+L L +F+ + RLD +ENLI + +FRG + +L L + + + + G L L L++N ++ I +L L N FC C L WL++ L+ R L ++ CT P L G + + + C
Sbjct: 32 GCPALCT----CSGTTVDCHGLALKGVPKNIPRSTERLELYGNNI--TRINKNDFS--GLKQ--LRVLQLMENQISNVERGAFDDMKELERLRLNRNQLHTLPELLFQN-NQALSRLDLSENLIQAIPRKTFRGATELKNLQLDKNQISCIEDGAFRALRG-LEVLTLNNNNITAIPVSSFNHMPKLRTFRLHSNNLFCDCHLAWLSQWLRQRPTI---GLFTQ--CTGPSPLRGLNVAEIQKHEFSC 262 HSP 2 Score: 57.7658 bits (138), Expect = 5.161e-8 Identity = 61/209 (29.19%), Postives = 84/209 (40.19%), Query Frame = 3
Query: 333 KLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKEL 956
KLT +P IP+ + ++ TL L P +L+ + L+ I IE AF ++ L L+ N L+ + SG+F+GL L L L NN + SF GL L L L NQ+S I + L ++L NPF C CQL WL L+ R K + RC NP L
Sbjct: 520 KLTKIPDRIPQSTAELRLNNNEI--ATLEATGLFRKLP---HLKKINLSNNKISEIEDAAFEGAASVSELHLTANHLESVRSGMFRGLEGLRTLMLRNNRVSCVHNDSFTGLRNVRL------------------------LSLYDNQISTITPGAFDTLQSLSTLNLLANPFNCNCQLAWLGDWLRKR-----KIVTGNPRCQNPDFL 694 HSP 3 Score: 56.225 bits (134), Expect = 1.753e-7 Identity = 66/256 (25.78%), Postives = 105/256 (41.02%), Query Frame = 3
Query: 264 AGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEG-SFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC--KPPGVKE 1022
CP+ C C +G + C LTA+P N+P+ + I + ++ A S Y LR + L+ I I P+AF L +L SL L N++ L G+F GL L L N + + +F+ L+NLS L L N++ + K ++ + L +NPF C C L+WL L+ A RC +P+ L+ + + + C K PG ++
Sbjct: 274 GSCPAMCT---CSNGIVDC-RGKGLTAIPANLPETMTEIRLELNGI--KSVPPGAFSPYK----KLRRIDLSNNQISEIAPDAFQGLRSLNSLVLYGNKITDLPKGVFSGLHALQLLLLNANKINCIRADTFQD------------LQNLSL------------LSLYDNKIQSLAKGTFAALRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETSGA-----RCASPRRLANKRIGQIKSKKFRCSGKMPGTED 490
BLAST of Slit-2 vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205]) HSP 1 Score: 70.4774 bits (171), Expect = 3.842e-12 Identity = 72/258 (27.91%), Postives = 117/258 (45.35%), Query Frame = 3
Query: 252 VEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLK----SCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
V P+A CP+ C SG V L ++P NIP+ + ++G N T + + L H LR+LQL I IE AF L L+ L L+ N L++ +F G + Y RLD +EN I + +FRG V + +L L SC +++ ++ +++ L L L++N ++ + +L L N +C C L WL+ L+ R L ++ C P L G + + + +C
Sbjct: 23 VAPQA-CPAQCS----CSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRH-------LRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLY-RLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISC-IEDGAFRALRD----LEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ--CMGPSHLRGHNVAEVQKREFVC 257 HSP 2 Score: 57.3806 bits (137), Expect = 5.008e-8 Identity = 70/267 (26.22%), Postives = 106/267 (39.70%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLE-GSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSC 1067
CP+ C S +V LT +P N+P+ I I + QN I + A S Y LR L L+ I + P+AF L +L SL L N++ L +F+GL L L N + L +F+ L +L L L N+L + K ++ + L +NPF C C L+WL L A RCT+P+ L+ + + + C G ++ L G+ L+C
Sbjct: 273 CPAACT----CSNNIVDCRGKGLTEIPTNLPETITEIRLE-QNSIRV-IPPGAFSPYK----KLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDL------------------------HNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGA-----RCTSPRRLANKRIGQIKSKKFRCS--GTEDYRSKLSGDCFADLAC 498 HSP 3 Score: 50.447 bits (119), Expect = 6.404e-6 Identity = 56/228 (24.56%), Postives = 91/228 (39.91%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNP 947
CP C+ + G V +L +P +IP+ + ++ F T L + P+ LR + + I +IE AF + + L++NRL+ + +FKGL SL L L +N + SF GL G + L L NQ++ + L ++L NPF C C L WL + L+ +++ + RC P
Sbjct: 497 ACPEKCRCE----GTTVDCSNQRLNKIPDHIPQYTAELRLNNNEF--TVLEATGIFKKLPQ---LRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGL------------------------GSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLR-----RKRIVTGNPRCQKP 686
BLAST of Slit-2 vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205]) HSP 1 Score: 70.0922 bits (170), Expect = 6.980e-12 Identity = 72/258 (27.91%), Postives = 117/258 (45.35%), Query Frame = 3
Query: 252 VEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLK----SCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
V P+A CP+ C SG V L ++P NIP+ + ++G N T + + L H LR+LQL I IE AF L L+ L L+ N L++ +F G + Y RLD +EN I + +FRG V + +L L SC +++ ++ +++ L L L++N ++ + +L L N +C C L WL+ L+ R L ++ C P L G + + + +C
Sbjct: 23 VAPQA-CPAQCS----CSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRH-------LRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLY-RLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISC-IEDGAFRALRD----LEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ--CMGPSHLRGHNVAEVQKREFVC 257 HSP 2 Score: 58.151 bits (139), Expect = 3.343e-8 Identity = 71/273 (26.01%), Postives = 107/273 (39.19%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLE-GSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKP------PGVKEIDIFLHGNNSGRLSC 1067
CP+ C S +V LT +P N+P+ I I + QN I + A S Y LR L L+ I + P+AF L +L SL L N++ L +F+GL L L N + L +F+ L +L L L N+L + K ++ + L +NPF C C L+WL L A RCT+P+ L+ + + + C PG ++ L G+ L+C
Sbjct: 277 CPAACT----CSNNIVDCRGKGLTEIPTNLPETITEIRLE-QNSIRV-IPPGAFSPYK----KLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDL------------------------HNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGA-----RCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLAC 510
BLAST of Slit-2 vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205]) HSP 1 Score: 69.707 bits (169), Expect = 7.658e-12 Identity = 72/258 (27.91%), Postives = 117/258 (45.35%), Query Frame = 3
Query: 252 VEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLK----SCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
V P+A CP+ C SG V L ++P NIP+ + ++G N T + + L H LR+LQL I IE AF L L+ L L+ N L++ +F G + Y RLD +EN I + +FRG V + +L L SC +++ ++ +++ L L L++N ++ + +L L N +C C L WL+ L+ R L ++ C P L G + + + +C
Sbjct: 23 VAPQA-CPAQCS----CSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRH-------LRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLY-RLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISC-IEDGAFRALRD----LEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ--CMGPSHLRGHNVAEVQKREFVC 257 HSP 2 Score: 56.9954 bits (136), Expect = 7.026e-8 Identity = 70/267 (26.22%), Postives = 106/267 (39.70%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLE-GSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSC 1067
CP+ C S +V LT +P N+P+ I I + QN I + A S Y LR L L+ I + P+AF L +L SL L N++ L +F+GL L L N + L +F+ L +L L L N+L + K ++ + L +NPF C C L+WL L A RCT+P+ L+ + + + C G ++ L G+ L+C
Sbjct: 273 CPAACT----CSNNIVDCRGKGLTEIPTNLPETITEIRLE-QNSIRV-IPPGAFSPYK----KLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDL------------------------HNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGA-----RCTSPRRLANKRIGQIKSKKFRCS--GTEDYRSKLSGDCFADLAC 498
BLAST of Slit-2 vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205]) HSP 1 Score: 69.707 bits (169), Expect = 8.068e-12 Identity = 72/258 (27.91%), Postives = 117/258 (45.35%), Query Frame = 3
Query: 252 VEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLK----SCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
V P+A CP+ C SG V L ++P NIP+ + ++G N T + + L H LR+LQL I IE AF L L+ L L+ N L++ +F G + Y RLD +EN I + +FRG V + +L L SC +++ ++ +++ L L L++N ++ + +L L N +C C L WL+ L+ R L ++ C P L G + + + +C
Sbjct: 23 VAPQA-CPAQCS----CSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRH-------LRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLY-RLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISC-IEDGAFRALRD----LEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ--CMGPSHLRGHNVAEVQKREFVC 257 HSP 2 Score: 56.9954 bits (136), Expect = 7.273e-8 Identity = 70/267 (26.22%), Postives = 106/267 (39.70%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLE-GSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSC 1067
CP+ C S +V LT +P N+P+ I I + QN I + A S Y LR L L+ I + P+AF L +L SL L N++ L +F+GL L L N + L +F+ L +L L L N+L + K ++ + L +NPF C C L+WL L A RCT+P+ L+ + + + C G ++ L G+ L+C
Sbjct: 277 CPAACT----CSNNIVDCRGKGLTEIPTNLPETITEIRLE-QNSIRV-IPPGAFSPYK----KLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDL------------------------HNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGA-----RCTSPRRLANKRIGQIKSKKFRCS--GTEDYRSKLSGDCFADLAC 502
BLAST of Slit-2 vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205]) HSP 1 Score: 69.707 bits (169), Expect = 8.442e-12 Identity = 72/258 (27.91%), Postives = 117/258 (45.35%), Query Frame = 3
Query: 252 VEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLK----SCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
V P+A CP+ C SG V L ++P NIP+ + ++G N T + + L H LR+LQL I IE AF L L+ L L+ N L++ +F G + Y RLD +EN I + +FRG V + +L L SC +++ ++ +++ L L L++N ++ + +L L N +C C L WL+ L+ R L ++ C P L G + + + +C
Sbjct: 23 VAPQA-CPAQCS----CSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRH-------LRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLY-RLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISC-IEDGAFRALRD----LEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ--CMGPSHLRGHNVAEVQKREFVC 257 HSP 2 Score: 57.7658 bits (138), Expect = 3.708e-8 Identity = 71/273 (26.01%), Postives = 107/273 (39.19%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLE-GSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKP------PGVKEIDIFLHGNNSGRLSC 1067
CP+ C S +V LT +P N+P+ I I + QN I + A S Y LR L L+ I + P+AF L +L SL L N++ L +F+GL L L N + L +F+ L +L L L N+L + K ++ + L +NPF C C L+WL L A RCT+P+ L+ + + + C PG ++ L G+ L+C
Sbjct: 277 CPAACT----CSNNIVDCRGKGLTEIPTNLPETITEIRLE-QNSIRV-IPPGAFSPYK----KLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDL------------------------HNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGA-----RCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLAC 510
BLAST of Slit-2 vs. UniProt/SwissProt
Match: sp|Q50LG9|LRC24_HUMAN (Leucine-rich repeat-containing protein 24 OS=Homo sapiens OX=9606 GN=LRRC24 PE=2 SV=2) HSP 1 Score: 83.1889 bits (204), Expect = 1.650e-15 Identity = 93/303 (30.69%), Postives = 137/303 (45.21%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLS-LDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKE----VSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELR---CTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGN--NSGRLSCEAINALETNVKWRHKLFASENYYRFQ 1145
GCP+ C+ C S + C +L +PL IP + + N L AL+ LR L L ++ +E AF L L L++NRL+ L SG F GL+ L L L N+ LL+ +F L L+ +L+ S E++++ L L LS NQL I ++ L+++ L ENP+ C C L WL +K ++ L S R C P L+ Q+L+ +S S+LIC PP V + L N R++C+A + V WR E R Q
Sbjct: 22 GCPAACR---CYSATVECGA-LRLRVVPLGIPPGTQTLFLQDNNI--ARLEPGALAPLA----ALRRLYLHNNSLRALEAGAFRAQPRLLELALTSNRLRGLRSGAFVGLAQLRVLYLAGNQLARLLDFTFLHLP----RLQELHLQENSIELLEDQALAGLSSLALLDLSRNQLGTISREALQPLASLQVLRLTENPWRCDCALHWLGAWIK---EGGQRLLTSRDRKIMCAEPPRLALQSLLDVSHSSLICIPPSVHVQPLELTANLGEDLRVACQASGYPQPLVTWRKVPQPREGRPRAQ 307
BLAST of Slit-2 vs. UniProt/SwissProt
Match: sp|P24014|SLIT_DROME (Protein slit OS=Drosophila melanogaster OX=7227 GN=sli PE=1 SV=2) HSP 1 Score: 75.8702 bits (185), Expect = 6.382e-13 Identity = 75/250 (30.00%), Postives = 110/250 (44.00%), Query Frame = 3
Query: 264 AGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVS----GHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
A CP C +G V LT++P I + + + G N T+ Y LR+LQLT I IE +F L++L+ L L+NNRLK + F S LRLD + N+I G R + + SL+S L N + E + L L L++N L+ + RL + L +NPF C C L WL++ L++ L RC +P +L GQ + L + C
Sbjct: 71 ARCPRVCS----CTGLNVDCSHRGLTSVPRKISADVERLELQGNNL---TVIYETDFQ---RLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPEN-FVTSSASLLRLDISNNVITTVG--RRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATR-----LAPYTRCQSPSQLKGQNVADLHDQEFKC 302 HSP 2 Score: 60.4622 bits (145), Expect = 3.999e-8 Identity = 76/252 (30.16%), Postives = 108/252 (42.86%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDN-NENLILLEGSFRGLVVSSL------SLKSCYLKNLSYEIIKEVSG-HLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
CP C+ C G + C E LT++P+ +P + + QNFI T L + S + LR + L+ NI I +A S L L +L L N++K L SG+FKGL L L N NE + + +FR L SL +++S L N +++ +K + HL K NPF C C LRWL L K S RC +PK + + + SL E C
Sbjct: 319 CPHPCR---CADGIVDCRE-KSLTSVPVTLPDDTTELRLE-QNFI-TELPPKSFSSF----RRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQS--LANGTFDAMKSIKTVHLAK-----------------------------NPFICDCNLRWLADYLH-----KNPIETSGARCESPKRMHRRRIESLREEKFKC 524
BLAST of Slit-2 vs. UniProt/SwissProt
Match: sp|Q9R1B9|SLIT2_MOUSE (Slit homolog 2 protein OS=Mus musculus OX=10090 GN=Slit2 PE=2 SV=2) HSP 1 Score: 69.707 bits (169), Expect = 5.357e-11 Identity = 72/258 (27.91%), Postives = 117/258 (45.35%), Query Frame = 3
Query: 252 VEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLK----SCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
V P+A CP+ C SG V L ++P NIP+ + ++G N T + + L H LR+LQL I IE AF L L+ L L+ N L++ +F G + Y RLD +EN I + +FRG V + +L L SC +++ ++ +++ L L L++N ++ + +L L N +C C L WL+ L+ R L ++ C P L G + + + +C
Sbjct: 23 VAPQA-CPAQCS----CSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRH-------LRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLY-RLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISC-IEDGAFRALRD----LEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ--CMGPSHLRGHNVAEVQKREFVC 257 HSP 2 Score: 56.9954 bits (136), Expect = 4.915e-7 Identity = 70/267 (26.22%), Postives = 106/267 (39.70%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLE-GSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSC 1067
CP+ C S +V LT +P N+P+ I I + QN I + A S Y LR L L+ I + P+AF L +L SL L N++ L +F+GL L L N + L +F+ L +L L L N+L + K ++ + L +NPF C C L+WL L A RCT+P+ L+ + + + C G ++ L G+ L+C
Sbjct: 273 CPAACT----CSNNIVDCRGKGLTEIPTNLPETITEIRLE-QNSIRV-IPPGAFSPYK----KLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDL------------------------HNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGA-----RCTSPRRLANKRIGQIKSKKFRCS--GTEDYRSKLSGDCFADLAC 498
BLAST of Slit-2 vs. UniProt/SwissProt
Match: sp|O94813|SLIT2_HUMAN (Slit homolog 2 protein OS=Homo sapiens OX=9606 GN=SLIT2 PE=1 SV=1) HSP 1 Score: 68.9366 bits (167), Expect = 1.066e-10 Identity = 72/258 (27.91%), Postives = 116/258 (44.96%), Query Frame = 3
Query: 252 VEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLK----SCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
V P+A CP+ C SG V L ++P NIP+ + ++G N T + L H LR+LQL I IE AF L L+ L L+ N L++ +F G + Y RLD +EN I + +FRG V + +L L SC +++ ++ +++ L L L++N ++ + +L L N +C C L WL+ L+ R L ++ C P L G + + + +C
Sbjct: 23 VAPQA-CPAQCS----CSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRH-------LRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLY-RLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISC-IEDGAFRALRD----LEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ--CMGPSHLRGHNVAEVQKREFVC 257 HSP 2 Score: 57.3806 bits (137), Expect = 3.291e-7 Identity = 71/273 (26.01%), Postives = 107/273 (39.19%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLE-GSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKP------PGVKEIDIFLHGNNSGRLSC 1067
CP+ C S +V LT +P N+P+ I I + QN I + A S Y LR + L+ I + P+AF L +L SL L N++ L +F+GL L L N + L +F+ L +L L L N+L I K ++ + L +NPF C C L+WL L A RCT+P+ L+ + + + C PG ++ L G+ L+C
Sbjct: 273 CPAACT----CSNNIVDCRGKGLTEIPTNLPETITEIRLE-QNTIKV-IPPGAFSPYK----KLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDL------------------------HNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGA-----RCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLAC 506
BLAST of Slit-2 vs. UniProt/SwissProt
Match: sp|Q9WVC1|SLIT2_RAT (Slit homolog 2 protein (Fragment) OS=Rattus norvegicus OX=10116 GN=Slit2 PE=1 SV=3) HSP 1 Score: 68.1662 bits (165), Expect = 1.282e-10 Identity = 73/258 (28.29%), Postives = 114/258 (44.19%), Query Frame = 3
Query: 252 VEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLI--LLEGSFRGLV-VSSLSLK----SCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
V P A CP+ C SG V L +P NIP+ + ++G N T + L H LRILQL I IE AF L L+ L L+ N L++ +F G + Y RLD +EN I + +FRG V + +L L SC +++ ++ +++ L L L++N ++ + +L L N +C C L WL+ L+ R L ++ C P L G + + + +C
Sbjct: 23 VAPHA-CPAQCS----CSGSTVDCHGLALRIVPRNIPRNTERLDLNGNNITRITKTDFAGLRH-------LRILQLMENKISTIERGAFHDLKELERLRLNRNNLQLFPELLFLGTAKLY-RLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISC-IEDGAFRALRD----LEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRV---GLYTQ--CMGPSHLRGHNVAEVQKREFVC 257 HSP 2 Score: 55.4546 bits (132), Expect = 1.204e-6 Identity = 70/267 (26.22%), Postives = 105/267 (39.33%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLE-GSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSC 1067
CP C S +V LT +P N+P+ I I + QN I + A S Y LR L L+ I + P+AF L +L SL L N++ L +F+GL L L N + L +F+ L +L L L N+L + K ++ + L +NPF C C L+WL L A RCT+P+ L+ + + + C G ++ L G+ L+C
Sbjct: 277 CPIACT----CSNNIVDCRGKGLTEIPTNLPETITEIRLE-QNSIRV-IPPGAFSPYK----KLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDL------------------------HNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGA-----RCTSPRRLANKRIGQIKSKKFRCS--GTEDYRSKLSGDCFADLAC 502 HSP 3 Score: 53.5286 bits (127), Expect = 4.487e-6 Identity = 58/228 (25.44%), Postives = 92/228 (40.35%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNP 947
CP C+ + G V KL +P +IP+ + ++ F T L + P+ LR + L+ I +IE AF + + L++NRL+ + +FKGL SL L L +N + SF GL G + L L NQ++ + L ++L NPF C C L WL + L+ +++ + RC P
Sbjct: 501 ACPEKCRCE----GTTVDCSNQKLNKIPDHIPQYTAELRLNNNEF--TVLEATGIFKKLPQ---LRKINLSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFTGL------------------------GSVRLLSLYDNQITTVAPGAFGTLHSLSTLNLLANPFNCNCHLAWLGEWLR-----RKRIVTGNPRCQKP 690
BLAST of Slit-2 vs. TrEMBL
Match: I1ZIL5 (Slit-2 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 227.639 bits (579), Expect = 9.754e-64 Identity = 164/474 (34.60%), Postives = 261/474 (55.06%), Query Frame = 3
Query: 264 AGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKA-LMSELRCTN-----PKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSCEAINALETNVKWRHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNIMWPTNN-------ISSFIYNSNEVRYIYPETFXXXXXXXXXXXPIEKFFMRKQFSLFELLISMVATLIVXXXXXXXXXXX---XXXXXXXPNLFQKSQPMTDSAVTSEVQMHNMSQFPSSQELKANQLLDFKTQQ 1637
+ CP C Q C L+C P L +P+N P+ I + +S QNF+ L+Y LS YG E+I L+ L ++ ++ +IE EAFSHLI L+ LDLS+NRL ++ FK LSL L LD+N NL L + SFRGLVV SLS+K C+LK L++E IK++SG+LT L L+ N+L + F+LLF L+ ++L+ N F C+CQL+WL +L+ R ++ + S LR TN PK L G ++ +LS + C+ P ++ IDI + + +SC + + + VKWR++ S +YR+ D + + ++ I + ++ D YSC + N G +D+ I WPT N I+S N+ T L + + ++ KQ++ E+++++ T I+T + L + + ++ N+ + + V SE Q +++ Q E +LLDFKTQQ
Sbjct: 33 SECPELCNQGACKLTMLLCETPG-LLQIPMNPPRGIQKVILSNQNFLYPRLTYTNLSRYGSEEIILKKLTISNCSLTSIESEAFSHLIYLQELDLSSNRLLFIYPNTFKSLSLKTLVLDSNRNLQLAKDSFRGLVVQSLSMKDCHLKILTHESIKQISGYLTTLNLADNRLEYLSNDFDLLFRGLDNLNLENNLFNCSCQLKWLALTLQTRTKRRDNSPKHSLLRLTNPVCEYPKNLKGSSIENLSIVSFQCQIPKLQRIDIKVTSEITAEISCISDDMSNSQVKWRYRYNLSPLFYRYPDQNSGVNQASIIVSKKSEFDFYSCLIINENGNATVDIKIKWPTLNKEYVESPINSPPNTVNQFNLRNNITKSSVILKEFDKLHVNYLWI-KQYTFIEMVLAICGTFIITLVTFILLYVVINYRRKNSRSKNILKG----YEVGVYSESQTYDVPQMSPYHESNTGRLLDFKTQQ 500
BLAST of Slit-2 vs. TrEMBL
Match: A0A5P8I4L0 (PXDN-like protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 186.808 bits (473), Expect = 1.298e-48 Identity = 125/399 (31.33%), Postives = 208/399 (52.13%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHA----KEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSC--EAINALETNVKWRHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNIMWPTNNISSFIYNSNEVRYIYPETFXXXXXXXXXXXPIEKFFMRKQFSLFELLISMVATLIV 1448
CPS C + C +G L C + L +P + + + I +S Q F + L+Y+ S+YGP + + L F + +I FSHL L+ LDLS+N L + FK L L +LRLD+N+ + SFRGL V+ LSL C L L +E+ E+S L KL L++N+L+ IDK+F +LFE L + + ENPF CTC LRWL L +R+ A K + ++ +C P + G+ + L + C P +K ID+F+ +S +L+C E +V W +E+ + ++ ++ +S+ ++ +D Y C ++N+ V + ++I WP +++ + N + + P N N+ + F KQF+L E++ +V T +
Sbjct: 26 CPSVCHEMACQTGVLACTD-GGLLQIPPVLHQKTHKIIVSNQRFSSPHLTYSNFSNYGPPNYKIEKIALQFCELVSIASNCFSHLTDLRVLDLSHNLLGEIPPDTFKALHLQFLRLDDNKMIQFHRFSFRGLNVAELSLFDCNLTELDFEVFSEISERLKKLILTNNKLNVIDKRFRILFENLPDLQINENPFNCTCNLRWLNSVLVSRKKAINLSSSKRIPNQAQCNYPDRVKGKQIADLDLKDFDCDSPRLKLIDVFMTSTSSAKLTCISEPKAHSSPDVIW-----MTEHKNTYVPEIQSIEKASLSVLRKSRIDKYKCVIKNNNDYVTVVISIHWPVKRVNNASQSLNTLG-VSPNDMIPYLPNANSTLHVRSVFYEKQFTLLEMIGGVVGTFTI 417
BLAST of Slit-2 vs. TrEMBL
Match: A0A2H1CUL7 (Leucine Rich repeat-containing domain protein OS=Fasciola hepatica OX=6192 GN=D915_01256 PE=4 SV=1) HSP 1 Score: 140.198 bits (352), Expect = 3.481e-32 Identity = 105/356 (29.49%), Postives = 181/356 (50.84%), Query Frame = 3
Query: 297 CLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGH--LTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQ-HAKEKAL---------------MSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSCEAINALET--NVKWRHKLFASEN----YYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNI-DVNIMWPTNNISS 1289
C++G L C E LT LP + K I + + G F + L+ + + Y + L+ + L IE I+P +F + +LK LDLS N++ + +G F GL LDYLRLD N L L G+F + SLS+ C +++++YE + + H LT L+LS N+L ++ + E +F L+ + L +NPF+C C+L WL + L+ RQ K +AL + C P L+G+ L +L+ + C P +K +++F+ + + +N++ V R ++ S +YR +SP + L E N +DH S + + ++ ++ ++ P+ +ISS
Sbjct: 5 CMAGSLSCQE-GGLTGLPDELAKDIQSMILIGHRFEGSALARHNFTSYPHQTKVLQRITLRNCGIERIQPGSFEPIQSLKQLDLSQNQINFIEAGTFAGLRLDYLRLDENHRLHLAPGAFNDASIVSLSMNQCGIESITYEDLAPLLRHHVLTTLHLSGNRLVTLENRMEPVFLELQSLSLDQNPFYCDCRLSWLAEVLQRRQIRRKSRALKQDSIMGPDAPLDSDLPRPTCRAPDRLAGRPLETLTTEDFFCGLPQLKNLEVFIEEYKEAGENQKLVNSVSQPLAVTLRCQVSGSPEMKLAWYRRSIFSPTVTSQL---EFYNELDHLSKLLSSRAVSSDVAEIRLIQPSPSISS 356
BLAST of Slit-2 vs. TrEMBL
Match: A0A4E0RLI5 (Immunoglobulin superfamily member 10 OS=Fasciola hepatica OX=6192 GN=D915_000869 PE=4 SV=1) HSP 1 Score: 140.969 bits (354), Expect = 2.003e-31 Identity = 105/356 (29.49%), Postives = 181/356 (50.84%), Query Frame = 3
Query: 297 CLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGH--LTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQ-HAKEKAL---------------MSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSCEAINALET--NVKWRHKLFASEN----YYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNI-DVNIMWPTNNISS 1289
C++G L C E LT LP + K I + + G F + L+ + + Y + L+ + L IE I+P +F + +LK LDLS N++ + +G F GL LDYLRLD N L L G+F + SLS+ C +++++YE + + H LT L+LS N+L ++ + E +F L+ + L +NPF+C C+L WL + L+ RQ K +AL + C P L+G+ L +L+ + C P +K +++F+ + + +N++ V R ++ S +YR +SP + L E N +DH S + + ++ ++ ++ P+ +ISS
Sbjct: 44 CMAGSLSCQE-GGLTGLPDELAKDIQSMILIGHRFEGSALARHNFTSYPHQTKVLQRITLRNCGIERIQPGSFEPIQSLKQLDLSQNQINFIEAGTFAGLRLDYLRLDENHRLHLAPGAFNDASIVSLSMNQCGIESITYEDLAPLLRHHVLTTLHLSGNRLVTLENRMEPVFLELQSLSLDQNPFYCDCRLSWLAEVLQRRQIRRKSRALKQDSIMGPDAPLDSDLPRPTCRAPDRLAGRPLETLTTEDFFCGLPQLKNLEVFIEEYKEAGENQKLVNSVSQPLAVTLRCQVSGSPEMKLAWYRRSIFSPTVTSQL---EFYNELDHLSKLLSSRAVSSDVAEIRLIQPSPSISS 395
BLAST of Slit-2 vs. TrEMBL
Match: A0A0X3PLP8 (Ig-like domain-containing protein OS=Schistocephalus solidus OX=70667 GN=TR119428 PE=4 SV=1) HSP 1 Score: 140.584 bits (353), Expect = 2.588e-31 Identity = 119/444 (26.80%), Postives = 208/444 (46.85%), Query Frame = 3
Query: 297 CLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEV--SGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELR---------------------CTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHG-----NNSG--------RLSCEAINALETNVKWRHKLFAS---------ENYYRFQDYSPALGRSLISIESRNNV----DHYSCYVQNSKGKVNIDVNIMW-----------PTNNISSFIYNSNEVRYIYPETFXXXXXXXXXXXPIEKFFMRKQFSLFELLISMVATLIV 1448
CL G + C EP +L +P ++P I + + GQ F + L LS Y ++ + L + ++ IEP+ F L L LD+S N L +H F GL L+ LRLD+N L L G+F L VSSLS+++C L++L+++ ++ + +G L+ L L+ N L+ ++ + E LF L+ + L +NPF C C+L WL+K L+ R+H E+ M + C +P L + L+ L++ + C PP ++ +++ L G N++G RL C A + E V W + S E+ Q SP + ++ + + D +C+ ++ G ++ DV + W + + I + V E+ + ++ FF RKQF++ EL+ +++ T +
Sbjct: 40 CLYGSVSCQEPGRLQMVPNDLPPGIRKVILIGQRFKSRYLQRQNLSSYSTSRVKIVELTIMDCGLQGIEPQTFQGLTNLTKLDVSKNALSRIHRRTFAGLRLELLRLDDNLGLSLSPGAFENLSVSSLSIQNCNLRSLTFKDLEPLIRTGTLSTLRLTGNSLTTLETRLEPLFLSLKALSLSQNPFRCDCELVWLSKMLQ-RKHQDEEQDMPPVPSGFENSLVNDDFTVSTEIYPVCASPPRLYERKLLGLTDEDFFCAPPQLRALELDLKGQQHAINSAGDSAGGAVARLKCIAQGSPELRVGWYRRTQRSDSKQGANFLESLLATQVVSPGVAEIVLHQPTSDGGSLEDDQLTCFGTDTYGNISADVTLSWRNLRESVNAGGASVGTAVAIAENGNVEEPDDESRLRALESER----MDDFFFRKQFTILELIGAVIGTFMA 478
BLAST of Slit-2 vs. Ensembl Cavefish
Match: mxra5a (matrix-remodeling-associated protein 5 [Source:NCBI gene;Acc:103036656]) HSP 1 Score: 71.2478 bits (173), Expect = 2.402e-12 Identity = 75/264 (28.41%), Postives = 119/264 (45.08%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN--TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLV-VSSLSLKSCYLKNLSYEIIKEVS--GH-----LTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEID 1028
CP C C K V L +P +PK + +++ G N +N T S+ + L +L + +I NI AF LI+L+ + +S N+LKI+ F+GL +L L LD+N + +F+GL + L L+ L+ L +S GH L LYLS N L+ + +K LE + L ENP+ C C+++W K+ L+C + L+G L + S K ++E+D
Sbjct: 21 CPRPCA---CPQPKEVHCTFRSLLTVPAGVPKQVERMNL-GFNTMNRITDSSFAGMR-------KLELLMMHGNDIHNIPNGAFRDLISLQMMKVSYNKLKIISRNTFQGLWNLARLHLDHNRLEFIHPDAFQGLTSLRLLQLEGNRLQQLHPATFATLSVLGHFPVSTLKHLYLSENGLTSLSQKMLAGMPHLENLFLHENPWVCDCRMKWFRDW--------SKSAPGVLKCKKDRALAGGQLCPMCSSPKHLKKKDLQELD 265
BLAST of Slit-2 vs. Ensembl Cavefish
Match: mxra5a (matrix-remodeling-associated protein 5 [Source:NCBI gene;Acc:103036656]) HSP 1 Score: 71.2478 bits (173), Expect = 2.408e-12 Identity = 75/264 (28.41%), Postives = 119/264 (45.08%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN--TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLV-VSSLSLKSCYLKNLSYEIIKEVS--GH-----LTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEID 1028
CP C C K V L +P +PK + +++ G N +N T S+ + L +L + +I NI AF LI+L+ + +S N+LKI+ F+GL +L L LD+N + +F+GL + L L+ L+ L +S GH L LYLS N L+ + +K LE + L ENP+ C C+++W K+ L+C + L+G L + S K ++E+D
Sbjct: 29 CPRPCA---CPQPKEVHCTFRSLLTVPAGVPKQVERMNL-GFNTMNRITDSSFAGMR-------KLELLMMHGNDIHNIPNGAFRDLISLQMMKVSYNKLKIISRNTFQGLWNLARLHLDHNRLEFIHPDAFQGLTSLRLLQLEGNRLQQLHPATFATLSVLGHFPVSTLKHLYLSENGLTSLSQKMLAGMPHLENLFLHENPWVCDCRMKWFRDW--------SKSAPGVLKCKKDRALAGGQLCPMCSSPKHLKKKDLQELD 273
BLAST of Slit-2 vs. Ensembl Cavefish
Match: mxra5a (matrix-remodeling-associated protein 5 [Source:NCBI gene;Acc:103036656]) HSP 1 Score: 71.2478 bits (173), Expect = 2.448e-12 Identity = 75/264 (28.41%), Postives = 119/264 (45.08%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN--TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLV-VSSLSLKSCYLKNLSYEIIKEVS--GH-----LTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEID 1028
CP C C K V L +P +PK + +++ G N +N T S+ + L +L + +I NI AF LI+L+ + +S N+LKI+ F+GL +L L LD+N + +F+GL + L L+ L+ L +S GH L LYLS N L+ + +K LE + L ENP+ C C+++W K+ L+C + L+G L + S K ++E+D
Sbjct: 29 CPRPCA---CPQPKEVHCTFRSLLTVPAGVPKQVERMNL-GFNTMNRITDSSFAGMR-------KLELLMMHGNDIHNIPNGAFRDLISLQMMKVSYNKLKIISRNTFQGLWNLARLHLDHNRLEFIHPDAFQGLTSLRLLQLEGNRLQQLHPATFATLSVLGHFPVSTLKHLYLSENGLTSLSQKMLAGMPHLENLFLHENPWVCDCRMKWFRDW--------SKSAPGVLKCKKDRALAGGQLCPMCSSPKHLKKKDLQELD 273
BLAST of Slit-2 vs. Ensembl Cavefish
Match: lrig2 (leucine rich repeats and immunoglobulin like domains 2 [Source:NCBI gene;Acc:103036991]) HSP 1 Score: 68.5514 bits (166), Expect = 1.167e-11 Identity = 83/299 (27.76%), Postives = 134/299 (44.82%), Query Frame = 3
Query: 459 LRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLS-LDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYL-KNLSYEIIK---EVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC----KP--PGVKEIDIFLHGNNSGRLSCEAI--NALETNVKWRHK---LFASE--NYYRFQDYSPALGRSLISIESRNNVD--HYSCYVQNSKGKVNIDVNIMWPTNNISSFI 1295
L L L+F ++ +E AF+ L L++L++ NRL L G+F GL+ + L + NN+ + +E S G+ V L + L KN I K E G L +L LS NQ+ + + + L + C CQL WL++ L + A + C +P L+G+++ ++ +C KP G + L G N L+C A + + WR L+ ++ NY R+Q+ L S++ + + N D HY C V N G N N + SF+
Sbjct: 272 LEELDLSFNHLSRLEDWAFTGLGLLQTLNVGENRLTHLGEGVFSGLANVHTLDIRNNDISLAIEDSV-GMFVGMKKLNTLILQKNKIRTITKRAFEGLGELEQLDLSKNQIMSVHPEAFSHLKLKSLDLNTSS-LLCDCQLTWLSRWLTNSHFQQSCAAV----CAHPAPLAGRSIFNVRPEEFVCNDFPKPQISGNPRTVVALRGTNV-TLNCSAFSSSDSTMSASWRKDREVLYEAQVHNYARYQEQR-LLYTSVLHLLNVNFTDEGHYQCVVSNHFGS-NYSTEAKLTVNELPSFL 561
BLAST of Slit-2 vs. Ensembl Cavefish
Match: slit3 (slit guidance ligand 3 [Source:NCBI gene;Acc:103037708]) HSP 1 Score: 67.781 bits (164), Expect = 2.200e-11 Identity = 67/255 (26.27%), Postives = 110/255 (43.14%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFIN-TTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLV-VSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKE 1022
GCP+ C SG V + ++P IP+ + + N T + ++ L NLR+L L I IE AF L L+ L L+ NRL++L +F+ L L L N+ + SFRG+ V +L L S ++ + + + L L L++N ++ I +L + L N C C L WL+ L+ R K L +C +P + G ++ + + + +C P E
Sbjct: 32 GCPAKCS----CSGSHVECQQQGFRSVPRGIPRNAERLDVDRNNITRITKVDFSGLK-------NLRVLHLENNQISVIERGAFQDLKQLERLRLNRNRLQVLPELLFQSTPKLGRLDLSENQIQAIPRKSFRGITNVKNLQLDSNHISCIEDGAFRALR-DLEILTLNNNNITLIPLSSFNHMPKLRTLRLHSNNLHCDCHLSWLSDWLRQR-----KGLAHFTQCMSPAHMRGLSVPDVQKRDFVCTGPAQSE 269 HSP 2 Score: 55.8398 bits (133), Expect = 1.152e-7 Identity = 64/252 (25.40%), Postives = 101/252 (40.08%), Query Frame = 3
Query: 258 PRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPP 1010
PR CP C S +V + L +LP IPK + + G + ++L L ++ L+ +I ++ P FS++ L +L LS N+++ + F GL SL L L N+ + EG+F HLT L SH L NP +C C+LRWL++ +KA KE + RCT P +++ + L++ + CK P
Sbjct: 724 PR--CPDPCT----CSDTVVRCSNHGLRSLPKGIPKDTTELYLEGNMLTSVPKELSSLKQ-------LSLVDLSNNSISSLTPFIFSNMTQLSTLILSYNQIRCVPVHAFDGLRSLRLLTLHGNDLSTIPEGAFN---------------------------HLTSL--SHLALGA-------------------NPLYCDCELRWLSQWVKA--GFKEPGIA---RCTGPPDMADRLLLTTPLNRFQCKGP 909 HSP 3 Score: 53.1434 bits (126), Expect = 8.877e-7 Identity = 68/252 (26.98%), Postives = 105/252 (41.67%), Query Frame = 3
Query: 255 EPRAGCP--SYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLE-GSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
EPR+ P S C + +V LT +P N+P+ I I + QN I + + A S Y L+ + L+ +I +I +AFS L +L SL L N++ + G+F GL L L N + L +F+ L SL+L L L N+L I K ++ + L +NPF C C L+WL L S RC++P+ L+ + + + C
Sbjct: 269 EPRSCAPQASVCPASCSCTNNIVDCRRKGLTEIPANLPEGIVEIRLE-QNLIKS-IPPGAFSPYK----KLKRIDLSKNHISDIADDAFSGLRSLTSLVLYGNKIAEIPKGLFDGLVSLQLLLLNANKINCLRVNTFKDL--QSLNL----------------------LSLYDNKLQTISKGLFTPLRAIKTLHLAQNPFMCDCHLKWLADYL-----FDNPIETSGARCSHPRRLANKRISQIKGKKFRC 485
BLAST of Slit-2 vs. Ensembl Sea Lamprey
Match: lrig3 (leucine-rich repeats and immunoglobulin-like domains 3 [Source:NCBI gene;Acc:564554]) HSP 1 Score: 71.633 bits (174), Expect = 2.097e-13 Identity = 87/323 (26.93%), Postives = 141/323 (43.65%), Query Frame = 3
Query: 393 QNFINTTLSYNALSHYGPEQI----NLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLS-LDYLRLDNNE---NLILLEGSFRGL-VVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC----KPPGV--KEIDIFLHGNNSGRLSCEAINALETNVK--WRHK---LFASENYYRFQDYSPALG--RSLISIESRNNVD-----HYSCYVQNS-----KGKVNIDVNIM 1265
Q+ + +S NA++ E NL L L+ + + AF L AL+SL L++N + + G F+GLS L L L N+ + ++G+F GL +S LSL +K+++ + + L +L L N + I + L + L + C CQL+WL L R A+ A+ C +P L G++++S+ + N +C KP V E + G+N + C A ++ E+ V WR L R + PA G S VD Y C + NS GK + V+++
Sbjct: 115 QSLRHLHVSRNAITAMDSEAWKFCENLWELDLSHNGLTRLNEAAFVELGALQSLSLAHNAISHIVEGAFRGLSSLRILDLGGNQISWTVEDMQGAFTGLSALSKLSLHGNQIKSIAKKAFVGLE-RLGQLDLMGNAIVSIQANAFNTLKLLHQMKLNTSELLCDCQLKWLPGWLTERGFAQSVAVA----CAHPAWLKGRSVLSVRQENFVCDDYPKPHMVVHPETRAAVRGSNVSFM-CSAASSSESPVTFLWRKDNAPLAVGAEVERLERARPAGGGLGEFTSYLHLRTVDFHHEGRYQCVISNSFGSSYSGKARLTVSVL 431
BLAST of Slit-2 vs. Ensembl Sea Lamprey
Match: pxdn (peroxidasin [Source:ZFIN;Acc:ZDB-GENE-140415-2]) HSP 1 Score: 65.855 bits (159), Expect = 2.089e-11 Identity = 74/269 (27.51%), Postives = 121/269 (44.98%), Query Frame = 3
Query: 474 LTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVS----GHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGV--KEIDIFLHGNNSGRLSCEAINALETNVKWRH---KLFASENYYR--FQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKV 1244
L F I I P AF L L +L L+NN +K + F+ L SL YL L NE + +F+GLV SL+ Y+ E+++ + L +L+L +N+LS I L + L N C C+L WL + L+A A ++ C +P+ L G+++ S++ C+ P + + D+ + N+ L C A + + W H ++ +E+ R QD G +I + Y C +N G+V
Sbjct: 6 LRFNRIREIPPGAFKKLKNLNTLLLNNNHIKRIPRRAFEDLESLKYLYLYKNEIQSIDRHAFKGLV----SLEQLYIHFNHIEVLEPNTFVDLPKLERLFLHNNRLSRIATGTFTQLSSLRRLRLDSNRLVCDCELVWLAELLRA--FAGSGGTLAAATCEHPRRLQGRSVSSITAQEFHCESPRITSEPHDVDVTPGNTVYLRCRAEGNPKPAIIWLHNSNEIAMAEDERRNLLQD-----GTLMIRDTRHTDQGTYQCMARNVAGEV 263
BLAST of Slit-2 vs. Ensembl Sea Lamprey
Match: lrfn5a (leucine rich repeat and fibronectin type III domain containing 5a [Source:ZFIN;Acc:ZDB-GENE-080327-17]) HSP 1 Score: 63.929 bits (154), Expect = 6.211e-11 Identity = 98/403 (24.32%), Postives = 163/403 (40.45%), Query Frame = 3
Query: 267 GCPSYCQQQ---PCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRI-LQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLS-LDYLRLDNNENLILLEGSFRGLVVSSLSLKSCY--LKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXEN-----------------------------------PFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEID---IFLHGNNSGRLSCEAINALETNVKW---RHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNIMWPTNNISSFIYNSNEVRYIYPE 1331
GCP++C Q P LS VC + L +P NI + + + G NFI + + +G + L + L L+ I I P AF+ L +L L NRL + + FKGLS L +L + NN+ + G+F ++S L Y L+ + +E I++++ L L L HN + I+ +L +D+ N P C C+L WL R+ +E L + C +P+ L G+ S+ E +C+PP + + L G + L C+AI + V W K+ ++ D G I + + + ++C N G+ V + +I + SN ++PE
Sbjct: 19 GCPTHCICQNLSPSLST--VCAKIG-LLFVPHNIDRRTVELRL-GDNFITSVRRQD----FG--NMTLMVDLTLSRNTISQIAPYAFADLSSLTFLHFDYNRLTHIGNKSFKGLSNLRHLIISNNQLSDISVGAFDDFLMSLEDLDLAYNNLEKVPWEAIQKMTS-LHTLSLDHNMIDYIEAGTFSHLHKLARLDMTSNKLQKLPPDPLFTRLQLFANKGIHHTPPLVLNFGGNPLHCNCELLWL------RRLTREDDLET---CASPQALVGKYFWSIPEEEFVCEPPLITRHTHRLLVLEGQRA-TLKCKAIGDPDPWVHWISPDEKVISNTTRTVVHDN----GTLEILVTTTKDDGTFTCIASNPAGESTTFVEL-----SIIHLPHLSNITHNVHPE 391
BLAST of Slit-2 vs. Ensembl Sea Lamprey
Match: LINGO2 (leucine rich repeat and Ig domain containing 2 [Source:HGNC Symbol;Acc:HGNC:21207]) HSP 1 Score: 56.6102 bits (135), Expect = 1.193e-8 Identity = 59/203 (29.06%), Postives = 92/203 (45.32%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQIN----LRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLS---------------LDY----------LRLDNNENLILLEGSFRGL-VVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGI 785
GCP+ C+ L +VC ++ A+P IP + LS N L P+ + L L++ I NIEP AF++L L+SL L NNRLK++ G+F L+ LDY L + +NE + + +F+GL + L+L+ C L +L E + + G L+ L L H ++ I
Sbjct: 36 GCPARCECT-ALQKSVVC-HRRRMVAIPEGIPTETKSLD----------LSKNRLRSLDPDDFSNFLLLEELEVNENVIANIEPGAFNNLPFLRSLSLRNNRLKLIPRGVFSRLTNLTHLDISENKIVILLDYMFQDLRNLKRLEVGDNELVYVSHHAFKGLQSLEQLTLEKCNLTSLPTEALSHL-GSLSTLRLRHLNIASI 225
BLAST of Slit-2 vs. Ensembl Sea Lamprey
Match: lrrc4ba (leucine rich repeat containing 4Ba [Source:ZFIN;Acc:ZDB-GENE-080327-25]) HSP 1 Score: 55.8398 bits (133), Expect = 1.924e-8 Identity = 69/289 (23.88%), Postives = 119/289 (41.18%), Query Frame = 3
Query: 456 NLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLS-LDYLRLDNNENLILLEGSFRGL-VVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPP--GVKEIDIFLHGNNSGRLSCEAINALETNVKWRHKLFASENYYRFQDYSPALGRSLISIESRNNVDH--YSCYVQNSKG--KVNIDVNIMWPTNNISSFIY 1298
NLR L L N+ + S L+ L+ L+LS NR++IL G F+GL+ L L + ++E ++ +F L + L+L L +L +++ + L +++L H NP+ C C + WL+ L R++ A RC P + L L S C PP V D+ + + L C A ++ ++V W S + ++ L ++ + D Y+C V NS G + +N+ P + + Y
Sbjct: 153 NLRYLNLGMCNLREVP--KLSPLVRLEELELSGNRIEILRPGSFQGLANLRKLWIMHSEVRLVERNAFDELQSLKELNLAHNSLMSLPHDLFSPLQ-KLERVHLHH------------------------NPWTCDCDILWLSWWL--RENVPNNATCCA-RCALPADRKAINLWELDSSRFTCYPPLIEVPPSDVNISEGMAAELRCRAPSS-ASSVTWITPNGTSMTHGSYKIRISVLHDGTLNFTNVTVQDTGIYTCLVANSVGNSTASATLNVTAPDTGTNGYTY 410
BLAST of Slit-2 vs. Ensembl Nematostella
Match: EDO47109 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNB0]) HSP 1 Score: 63.5438 bits (153), Expect = 1.428e-10 Identity = 59/206 (28.64%), Postives = 92/206 (44.66%), Query Frame = 3
Query: 399 FINTTLSYNALSHYGPEQINLRILQLTFTNIE---NI-EPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFR-GLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
I +S+N LS + E LR L T T + NI A + LDL +N L++ F GLSL LRLD N+ + E + G + L + YL ++ + + ++ + +L L+ N +S I ++L I L NP C C+L WL L +RQ + C +P + GQT + L + +C
Sbjct: 170 LICLDISFNRLSEFKTE--TLRGLSSTLTELRLRSNILRRVAIKRRFNITLLDLGDNLLEVFEVDAFDGLSLRKLRLDGNKLDEIPEAFAKVGHFLQELDMSGNYLHEITSDQLLDLHA-IERLDLNENNISKIRIGALRGMKKLRQISLSGNPLSCDCRLVWLKHVLDSRQDQLHLSSPQSTTCASPASVKGQTFLGLDARSFLC 372 HSP 2 Score: 51.9878 bits (123), Expect = 5.744e-7 Identity = 45/156 (28.85%), Postives = 78/156 (50.00%), Query Frame = 3
Query: 336 LTALPLNIPKIIYHISISGQNFINT----TLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFR-GLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGI 785
L +P ++P+ + G F+NT L A ++Y ++LR+L L+ + I F L LK+L L++NRL+++++ F+GL L YL L +N + +G+FR + L + L E ++ +S LT+L L N L +
Sbjct: 63 LQTIPTSLPRNL------GSLFLNTNHIRALQAKAFANY----LSLRVLDLSNNELSVIANGTFKSLGKLKTLTLNSNRLEVVYAETFRGLRDLRYLSLRHNSIRTIGDGAFRFSAFLICLDISFNRLSEFKTETLRGLSSTLTELRLRSNILRRV 208
BLAST of Slit-2 vs. Ensembl Nematostella
Match: EDO48179 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RK32]) HSP 1 Score: 61.2326 bits (147), Expect = 8.685e-10 Identity = 60/235 (25.53%), Postives = 108/235 (45.96%), Query Frame = 3
Query: 459 LRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLE---GSFRGLV-VSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICK-----------PPGVKEIDIFLHGNNSG---RLSCEAINALETNVKWR 1106
LR L L + +I+ F HL+ALK L+L+NNR+ + G F L SL+ L L NN+ +E G FRGL ++SL L + ++ + ++ L LS N ++ I + ++L+ + L + C C+++W L++R + RC +P+ L +++ +++ +C PP K + G+N R+ N+ ++WR
Sbjct: 348 LRKLNLERNRLSHIQDTTFKHLLALKVLNLANNRIYHISQGSFSDLRSLEGLDLSNNDISWTVEEMNGPFRGLTNLASLRLDGNRITAIAATAFLGLE-NIKYLNLSANIITSIQENSFQGMDKLQKLWLNTSQLMCDCKIKWFGSWLRSRPSTRHTV---RARCLHPQTLYMKSVFNITPDAFVCDTSNPIPQISVHPPSQKA----MRGDNVTLRCRVQVVQRNSSSVRLQWR 574
BLAST of Slit-2 vs. Ensembl Nematostella
Match: EDO35407 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLJ8]) HSP 1 Score: 48.521 bits (114), Expect = 2.868e-6 Identity = 66/235 (28.09%), Postives = 95/235 (40.43%), Query Frame = 3
Query: 459 LRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENL--ILLEGSFRGLV------VSSLSLKSC------------------------------------YLK--NLSYEIIKEVSG-------HLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
L+ L L I + F L++L+ LDL N LK L G+FK L SL L L +N N+ + EG+F GL VS+ +K +LK +LS +I VS L KL L +N + I + + L + L+ NPF C CQL L+ Q+ + C P +L+G+ L+ LS S+L C
Sbjct: 44 LKQLNLGENKIRTVSASMFKGLVSLEKLDLRFNDLKALPRGVFKPLKSLRELFLHDNRNMGSRIEEGAFNGLDNLRKLHVSATGMKKITQGTFGSLMSLEFLDVLHINNNSLTTVPLHALRKVKFLKELDLSANLISYVSSTAFANNSKLQKLLLHNNTIRLIHEDAFSQLKGLRALFLRGNPFQCDCQLNGFHAWLRNNQYLVN--FNGHMYCMTPDKLTGRDLLELSSSDLKC 276
BLAST of Slit-2 vs. Ensembl Medaka
Match: ENSORLT00000000072.2 (pep primary_assembly:ASM223467v1:1:1576629:1604541:-1 gene:ENSORLG00000000055.2 transcript:ENSORLT00000000072.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 68.5514 bits (166), Expect = 4.903e-12 Identity = 71/248 (28.63%), Postives = 106/248 (42.74%), Query Frame = 3
Query: 267 GCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLS-YNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIF-KGLSLDYLRLDNNENLILLEGSFRGLV-VSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLIC 1001
GCP+ C +G V + ++P NIP+ + ++G N T S + L H LR+L L I NIE AF L L+ L L+ NRL L +F K +L L L N + +FRG + +L L ++ + + + L L L++N +S I +L L N C C L WL+ L+ R AL +C++P L G L L +S+ C
Sbjct: 44 GCPAPCT----CAGNTVDCHGRGIQSVPKNIPRGTERLDLNGNNLTVITKSDLSGLRH-------LRVLHLMENQISNIERGAFDELKELERLRLNRNRLSQLPELLFQKNEALSRLDLSENRIQAVPRRAFRGAADLKNLQLDKNHISCVEEGAFRALR-SLEVLTLNNNNISSIPVSSFNHMPKLRTFRLHSNSLRCDCHLAWLSPWLRQR-----PALGLYTQCSSPAALRGLNLAELRKSDFAC 274
BLAST of Slit-2 vs. Ensembl Medaka
Match: si:cabz01090165.1 (leucine-rich repeat and fibronectin type III domain-containing protein 1-like protein [Source:NCBI gene;Acc:101172994]) HSP 1 Score: 67.781 bits (164), Expect = 1.933e-11 Identity = 80/325 (24.62%), Postives = 133/325 (40.92%), Query Frame = 3
Query: 249 CVEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLV--------------------------VSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIX-----------XXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKE--IDIFLHGNNSGRLSCEAINALETNVKW 1103
CV P CP C Q L V L +P NI + + + NFI TTL + ++ +L L L+ I I P F+ L L +L + NRL +L F+GL +L +L L NN+ + EG+F+ + V++LSL ++++ I + L +L ++ N+L I K + ++ NP C C+L WL R+ +E L + C +PK+L+G+ ++ E +C+PP + +F+ L C++I E + W
Sbjct: 20 CV-PSMLCPKRCTCQNLLPSYTVLCAKTGLLFVPPNIDRQTAELRLM-DNFI-TTLRHRDFANMS----SLIHLTLSRNTISQIRPYTFADLQELHALHMDANRLTVLDDSHFQGLVNLRHLILANNQLHSISEGAFQDFLETLEDLDLSYNNLVDIPWDTIALLVSVNTLSLDHNLIESVPEGIFSNLH-KLARLDMTSNKLKKIPPDPLFLRIPVYAKMKGSPLTALVLSFGGNPLHCNCELVWL------RRVTREDDLET---CASPKDLAGKYFWTIREEEFVCEPPMITRHTSKMFVMEGQEVSLRCKSIGDPEPSTHW 327
BLAST of Slit-2 vs. Ensembl Medaka
Match: slitrk5a (SLIT and NTRK like family member 5 [Source:NCBI gene;Acc:101159898]) HSP 1 Score: 63.929 bits (154), Expect = 3.007e-10 Identity = 70/230 (30.43%), Postives = 114/230 (49.57%), Query Frame = 3
Query: 333 KLTAL-PLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGL-VVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIX-XXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPP 1010
+LT + P+ P +YH+ ++G + LS N +Y + IL L +I IE AF+ L LK L L+NN+++IL F GL SL+YL++D N I+ + L ++ + L L +L I + V LT L L N+L +++ + L+ENP+ C+C+L L L++ + AL+ E+ C P L G+ L +S+ L + P
Sbjct: 53 RLTEISPVRFP--MYHLLLTGN--LLKKLSVNDFINY----TGVTILHLGNNDISEIESGAFNGLQGLKRLHLNNNKIEILRDDTFAGLESLEYLQIDYNYVSIIEPNALSRLHQLTVMILNDNLLSSLPPNIFRNVP--LTHLDLRGNRLKMFPYIGLLEHMDKVVELQLEENPWNCSCELIALKAWLESIAYT---ALVGEVVCETPFRLHGRDLDEVSKQELCPRRP 269
BLAST of Slit-2 vs. Ensembl Medaka
Match: pxdn (peroxidasin [Source:NCBI gene;Acc:101162089]) HSP 1 Score: 63.929 bits (154), Expect = 3.378e-10 Identity = 73/273 (26.74%), Postives = 129/273 (47.25%), Query Frame = 3
Query: 465 ILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVS-GHLTKL---YLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGV----KEIDIFLHGNNSGRLSCEAINALETNVKWRHKLFASENYYRFQDYSP--ALGRSLISIESRNNVDH--YSCYVQNSKGKV 1244
IL L F I++++P +FS L L +L L+NN+++ + G F+ L +L YL L NE + +F+GLV SL+ YL + E ++ S HL KL +L +N+++ + ++ + L N C C++ WL LK +A+ + C +P L G+++ +L+ L C+ P + +++D+ L N+ +C A + + W + N +D S L + I+ D Y C +N G+V
Sbjct: 64 ILDLRFNKIKDLQPASFSRLKNLNTLLLNNNQIRRISRGAFEDLENLKYLYLYKNEIQSIDRQAFKGLV----SLEQLYLHFNNIESLEPESFSHLPKLERLFLHNNRITQLVPGTFSHLSAMKRLRLDSNALNCNCEVLWLADLLKL--YAESGNAQAAATCDHPSRLQGRSVATLTAEELNCELPRITSEPQDVDVTL--GNTVYFTCRAEGNPKPQIIW----LRNNNALNMRDDSRLNLLEDGTLMIQDTRETDQGVYQCMAKNVAGEV 324 HSP 2 Score: 51.6026 bits (122), Expect = 2.153e-6 Identity = 30/59 (50.85%), Postives = 39/59 (66.10%), Query Frame = 3
Query: 453 INLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLS-LDYLRLDNN 626
++L L L F NIE++EPE+FSHL L+ L L NNR+ L G F LS + LRLD+N
Sbjct: 132 VSLEQLYLHFNNIESLEPESFSHLPKLERLFLHNNRITQLVPGTFSHLSAMKRLRLDSN 190
BLAST of Slit-2 vs. Ensembl Medaka
Match: lrfn1 (leucine rich repeat and fibronectin type III domain containing 1 [Source:NCBI gene;Acc:101167138]) HSP 1 Score: 63.5438 bits (153), Expect = 4.011e-10 Identity = 82/328 (25.00%), Postives = 128/328 (39.02%), Query Frame = 3
Query: 468 LQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGL-SLDYLRLDNNENLILLEGSFRGLV--VSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHN-----------------------------------QLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKE---IDIFLHGNNSGRLSCEAINALETNVKWRH---KLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNI-MWPTNNISSFIYNSNEVR 1316
L L+ I I P AF L +L++L + NRL I+ S FKGL +L +L L NN+ + SF V + L L + L+ L +E I +S ++ L L HN Q + + + + + NP C C+L WL R+ +E L + C +P+ L + S+ E IC+PP + + ++ L C+A+ + + W+ KL N R Y LIS N Y+C N+ G V I M P + F+ N+ +R
Sbjct: 80 LTLSRNTISQIVPHAFVGLRSLRALHMDGNRLNIIKSDHFKGLINLRHLILGNNQIHEVASTSFDEFVSTIEDLDLSNNNLRGLPWEAIARMS-NINTLTLDHNLIDHIEAGTFTLLTKLVRLDMTSNRLQKLPPDSLFQHAQVLSEVKGSSSSTLAVSFGGNPLHCNCELLWL------RRLTREDDLET---CASPEHLMDKYFWSIQEEEFICEPPLITKHVASKSYVMEGQGVTLKCKAVGDPDPFIHWQSPDGKLV--HNNSRTILYDNGTLDILISTLKDNGA--YNCVASNAAGIATAAVEITMIP---LPLFVNNTGHMR 390
BLAST of Slit-2 vs. Planmine SMEST
Match: SMESG000002157.1 (SMESG000002157.1) HSP 1 Score: 895.19 bits (2312), Expect = 0.000e+0 Identity = 515/528 (97.54%), Postives = 521/528 (98.67%), Query Frame = 3
Query: 111 YIKINSMRF----FYRCKYNLEGVNFSDIKIYSXXXXXXXXXXXXXXXXHCVEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSCEAINALETNVKWRHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNIMWPTNNISSFIYNSNEVRYIYPETFXXXXXXXXXXXPIEKFFMRKQFSLFELLISMVATLIVXXXXXXXXXXXXXXXXXXPNLFQKSQPMTDSAVTSEVQMHNMSQFPSSQELKANQLLDFKTQQILPLVMSQSNWSLKI 1682
Y+ S++F ++RCKYNLEGVNFSDIKIYSITTNIVLIIIHILHNIHCVEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRL+ILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIDKKFELLFERLELIDLKENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSCEAINALETNVKWRHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNIMWPTNNISSFIYNSNEVRYIYPETFDDNQLNLNNDNPIEKFFMRKQFSLFELLISMVATLIVTSLLSTLCFCLFFRHRFLPNLFQKSQPMTDSAVTSEVQMHNMSQFPSSQELKANQLLDFKTQQILPLVMSQSNWSLKI
Sbjct: 28 YLSNPSLKFVFKGYFRCKYNLEGVNFSDIKIYSITTNIVLIIIHILHNIHCVEPRAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLRILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIDKKFELLFERLELIDLKENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSCEAINALETNVKWRHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNIMWPTNNISSFIYNSNEVRYIYPETFDDNQLNLNNDNPIEKFFMRKQFSLFELLISMVATLIVTSLLSTLCFCLFFRHRFLPNLFQKSQPMTDSAVTSEVQMHNMSQFPSSQELKANQLLDFKTQQILPLVMSQSNWSLKI 555
BLAST of Slit-2 vs. Planmine SMEST
Match: SMESG000033458.1 (SMESG000033458.1) HSP 1 Score: 237.269 bits (604), Expect = 6.956e-71 Identity = 167/477 (35.01%), Postives = 263/477 (55.14%), Query Frame = 3
Query: 264 AGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKA-LMSELRCTN-----PKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSCEAINALETNVKWRHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNIMWPTNNISSFIYNSNEVRYIYPET---FXXXXXXXXXXXPIEKF-------FMRKQFSLFELLISMVATLI---VXXXXXXXXXXXXXXXXXXPNLFQKSQPMTDSAVTSEVQMHNMSQFPSSQELKANQLLDFKTQQ 1637
+ CP C Q C L+C P L +P+N P+ I + +S QNF+ L+Y LS YG E+I L+ L ++ ++ +IE EAFSHLI L+ LDLS+NRL ++ FK LSL L LD+N NL L + SFRGLVV SLS+K C+LK L++E IK++SG+LT L L+ N+L + F+LLF L+ ++L+ NPF C+CQL+WL +L+ R ++ + S LR TN PK L G ++ +LS + C+ P ++ IDI + + +SC + + + VKWR++ S +YR+ D + L ++ I + ++ D YSC + N G +D+ I WPT N Y + ++ P T F+ + +++F KQ++ E+++++ T I VT +L + ++ N+ + + V SE Q +++ Q E +LLDFKTQQ
Sbjct: 33 SECPELCNQGACKLTMLLCETPG-LLQIPMNPPRGIQKVILSNQNFLYPRLTYTNLSRYGSEEIILKKLTISNCSLTSIESEAFSHLIYLQELDLSSNRLLFIYPNTFKSLSLKTLVLDSNRNLQLAKDSFRGLVVQSLSMKDCHLKILTHESIKQISGYLTTLNLADNRLEYLSNDFDLLFRGLDNLNLENNPFNCSCQLKWLALTLQTRTKRRDNSPKHSLLRLTNPVCEYPKNLKGSSIENLSIVSFQCQIPKLQRIDIKVTSEITAEISCISDDMSNSQVKWRYRYNLSPLFYRYPDQNSGLNQASIIVSKKSEFDFYSCLIINENGNATVDIKIKWPTLNKE---YVESPIKSP-PNTVNQFNLRNNITKSSVILKEFDKLHVNYLWIKQYTFIEMVLAICGTFIITLVTFILLYVVINYRRKNSRSKNILKGYE----VGVYSESQTYDVPQMSPYHESNTGRLLDFKTQQ 500
BLAST of Slit-2 vs. Planmine SMEST
Match: SMESG000033458.1 (SMESG000033458.1) HSP 1 Score: 208.764 bits (530), Expect = 1.665e-60 Identity = 157/477 (32.91%), Postives = 250/477 (52.41%), Query Frame = 3
Query: 264 AGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKA-LMSELRCTNP-----KELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSCEAINALETNVKWRHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNIMWPTNNISSFIYNSNEVRYIYPET---FXXXXXXXXXXXPIEKF-------FMRKQFSLFELLISMVATLI---VXXXXXXXXXXXXXXXXXXPNLFQKSQPMTDSAVTSEVQMHNMSQFPSSQELKANQLLDFKTQQ 1637
+ CP C Q C L+C P L +P+N P+ I + +S QNF+ L+Y LS YG E+I L+ L ++ ++ +IE EAFSHLI L+ LDLSN+ N NL L + SFRGLVV SLS+K C+LK L++E IK++SG+LT L L+ N+L + F+LLF L+ ++L+ NPF C+CQL+WL +L+ R ++ + S LR TNP K L G ++ +LS + C+ P ++ IDI + + +SC + + + VKWR++ S +YR+ D + L ++ I + ++ D YSC + N G +D+ I WPT N Y + ++ P T F+ + +++F KQ++ E+++++ T I VT +L + ++ N+ + + V SE Q +++ Q E +LLDFKTQQ
Sbjct: 33 SECPELCNQGACKLTMLLCETPG-LLQIPMNPPRGIQKVILSNQNFLYPRLTYTNLSRYGSEEIILKKLTISNCSLTSIESEAFSHLIYLQELDLSNS----------------------NRNLQLAKDSFRGLVVQSLSMKDCHLKILTHESIKQISGYLTTLNLADNRLEYLSNDFDLLFRGLDNLNLENNPFNCSCQLKWLALTLQTRTKRRDNSPKHSLLRLTNPVCEYPKNLKGSSIENLSIVSFQCQIPKLQRIDIKVTSEITAEISCISDDMSNSQVKWRYRYNLSPLFYRYPDQNSGLNQASIIVSKKSEFDFYSCLIINENGNATVDIKIKWPTLNKE---YVESPIKSP-PNTVNQFNLRNNITKSSVILKEFDKLHVNYLWIKQYTFIEMVLAICGTFIITLVTFILLYVVINYRRKNSRSKNILKG----YEVGVYSESQTYDVPQMSPYHESNTGRLLDFKTQQ 478
BLAST of Slit-2 vs. Planmine SMEST
Match: SMESG000026986.1 (SMESG000026986.1) HSP 1 Score: 206.068 bits (523), Expect = 1.603e-59 Identity = 148/477 (31.03%), Postives = 247/477 (51.78%), Query Frame = 3
Query: 261 RAGCPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHAKEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSCEAINALETNVKWRHKLFASENYYRFQDYSPALGRSLISIESRNNVDHYSCYVQNSKGKVNIDVNIMWPTNNISSFIYNSNEV-----------RYIYPETFXXXXXXXXXXXPIEKFFMRKQFSLFELLISMVATLIVXXXXXXXXXXXXXXXXXXPNLFQKSQPMT---DSAVTSEVQMHNMSQF--PSSQELKANQLLDFKTQQIL 1643
+A CP C Q C G L C++ L +P N P + + Q F N L Y LS YG E+I L++L + + IE E+FSHL+ L +DLS N+L++++ FK L+L L+L+ N L L SF+GLVVSSLSL +C L +L ++ IKE++GHL + LS N L ++ +F LLF+RLE I L N C C+++WL + LK++ +K ++ S ++C+ P E+ G+ + L ES +C P +K I + + S L C + +T V W + + + ++ +D + I + + D +SC V+N G V++D+NI WP N + + + I P+T + KQF+ E++IS+ +T +VT++ +L + R + K P + S+ Q+++ ++ P+ ++ QL+DFKTQ+++
Sbjct: 18 QANCPVLCNQNSCTYGFLFCVDIG-LEMIPENPPPKTTQVIVKNQIFKNPILGYKNLSRYGTEEILLKVLTIQNCGLTEIESESFSHLVHLMEMDLSFNQLQMIYPRTFKALNLKSLKLNGNRLLKLTGNSFQGLVVSSLSLANCGLDSLEFDSIKEIAGHLIRFDLSQNDLKSLNFRFYLLFKRLEEIQLGGNQLKCDCRIKWLNRILKSKNQSKA-SIESLIKCSQPYEIRGKAISQLEESQFLCSKPNLKAISVDI-STRSAILYCSSSPNSDTQVSWHLRNAVNYSLHKLEDLIETPFNASIKVNKNSQSDMFSCIVKNENGNVSVDLNIKWPENTSETLTDKGHPILLFSTPSNNSSTSIIPQT--------------TNYLYIKQFTFLEMIISIGSTFVVTTITFSLIYLCLSRRLNIS--IGKQSPCKIGYQGGIYSDTQIYDSPRYSTPNKTFQQSAQLIDFKTQRLI 475
BLAST of Slit-2 vs. Planmine SMEST
Match: SMESG000026559.1 (SMESG000026559.1) HSP 1 Score: 184.111 bits (466), Expect = 3.071e-51 Identity = 128/404 (31.68%), Postives = 206/404 (50.99%), Query Frame = 3
Query: 270 CPSYCQQQPCLSGKLVCIEPNKLTALPLNIPKIIYHISISGQNFINTTLSYNALSHYGPEQINLRILQLTFTNIENIEPEAFSHLIALKSLDLSNNRLKILHSGIFKGLSLDYLRLDNNENLILLEGSFRGLVVSSLSLKSCYLKNLSYEIIKEVSGHLTKLYLSHNQLSGIXXXXXXXXXXXXXXXXXENPFFCTCQLRWLTKSLKARQHA----KEKALMSELRCTNPKELSGQTLISLSESNLICKPPGVKEIDIFLHGNNSGRLSC--EAINALETNVKW--RHKLFASENYYRFQDYSPALGRS---LISIESRNNVDHYSCYVQNSKGKVNIDVNIMWPTNNISSFIYNSNEVRYIYPETFXXXXXXXXXXXPIEKFFMRKQFSLFELLISMVATLIV 1448
CPS C + C +G L C + L +P + + + I +S Q F + L+Y+ S+YGP + + L F + +I FSHL L+ LDLS+N L + FK L L +LRLD+N+ + SFRGL V+ LSL C L L +E+ E+S L KL L++N+L+ IDK+F +LFE L + + ENPF CTC LRWL L +R+ A K + ++ +C P + G+ + L + C P +K ID+F+ +S +L+C E +V W H+ Y P + S +S+ ++ +D Y C ++N+ V + ++I WP +++ + N + + P N N+ + F KQF+L E++ +V T +
Sbjct: 26 CPSVCHEMACQTGVLACTD-GGLLQIPPVLHQKTHKIIVSNQRFSSPHLTYSNFSNYGPPNYKIEKIALQFCELVSIASNCFSHLTDLRVLDLSHNLLGEIPPDTFKALHLQFLRLDDNKMIQFHRFSFRGLNVAELSLFDCNLTELDFEVFSEISERLKKLILTNNKLNVIDKRFRILFENLPDLQINENPFNCTCNLRWLNSVLVSRKKAINLSSSKRIPNQAQCNYPDRVKGKQIADLDLKDFDCDSPRLKLIDVFMTSTSSAKLTCISEPKAHSSPDVIWMAEHR----------NTYVPEIQSSENASLSVLRKSRIDKYKCVIKNNNDYVTVVISIHWPVKRVNNASQSLNTLG-VSPNDMIPYLPNANSTLHVRSVFYEKQFTLLEMIGGIVGTFTI 417 The following BLAST results are available for this feature:
BLAST of Slit-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Slit-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of Slit-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Slit-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Slit-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Slit-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Slit-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Slit-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Slit-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Slit-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Slit-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Slit-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 3
BLAST of Slit-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Slit-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30034592 ID=SMED30034592|Name=Slit-2|organism=Schmidtea mediterranea sexual|type=transcript|length=1769bpback to top protein sequence of SMED30034592-orf-1 >SMED30034592-orf-1 ID=SMED30034592-orf-1|Name=SMED30034592-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=519bp MRFFYRCKYNLEGVNFSDIKIYSITTNIVLIIIHILHNIHCVEPRAGCPSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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