Tubulin beta chain

Overview
NameTubulin beta chain
Smed IDSMED30034573
Length (bp)2309
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Tubulin beta chain (SMED30034573) t-SNE clustered cells

Violin plots show distribution of expression levels for Tubulin beta chain (SMED30034573) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Tubulin beta chain (SMED30034573) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Tubulin beta chain (SMED30034573) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30034573

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30034573SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 Contig2059GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30034573SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 Contig2059GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
headSMED30034573SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 Contig2059GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
pharynxSMED30034573SMESG000047291.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 SMESG000047284.1 Contig5821GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30034573SMESG000047291.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 SMESG000047284.1 Contig5821GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30034573SMESG000047291.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 SMESG000047284.1 Contig5821GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
central nervous systemSMED30034573SMESG000047291.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 SMESG000047284.1 Contig5821GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
headSMED30034573SMESG000047291.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 SMESG000047284.1 Contig5821GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30034573SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 SMED_32051_V2_1rGPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30034573SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 SMED_32051_V2_1rGPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB4B (tubulin beta 4B class IVb [Source:HGNC Symbol;Acc:HGNC:20771])

HSP 1 Score: 839.721 bits (2168), Expect = 0.000e+0
Identity = 413/427 (96.72%), Postives = 424/427 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 1379
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB2B (tubulin beta 2B class IIb [Source:HGNC Symbol;Acc:HGNC:30829])

HSP 1 Score: 837.795 bits (2163), Expect = 0.000e+0
Identity = 410/430 (95.35%), Postives = 425/430 (98.84%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATG KYVPRA+L+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSV+PSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 833.943 bits (2153), Expect = 0.000e+0
Identity = 408/429 (95.10%), Postives = 425/429 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQL+RI+VYYNEATGGKYVPRA+L+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 833.943 bits (2153), Expect = 0.000e+0
Identity = 408/429 (95.10%), Postives = 425/429 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQL+RI+VYYNEATGGKYVPRA+L+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 833.943 bits (2153), Expect = 0.000e+0
Identity = 408/429 (95.10%), Postives = 425/429 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQL+RI+VYYNEATGGKYVPRA+L+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-4 (Tubulin beta-4 chain [Source:UniProtKB/Swiss-Prot;Acc:P41937])

HSP 1 Score: 830.091 bits (2143), Expect = 0.000e+0
Identity = 406/430 (94.42%), Postives = 424/430 (98.60%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+Y+GDSDLQLERINVYYNEA+GGKYVPRA L+DLEPG MDSVR+GPFGQLFRPDNF+FGQSGAGNNWAKGHYTEGAELVD+VLD VRKEAESCDCLQGFQ+THSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYR+LTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRG+KM+ATF+GNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGAYNGDSDLQLERINVYYNEASGGKYVPRACLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAESCDCLQGFQMTHSLGGGTGSGMGTLLISKIREEYPDRIMMTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSMTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRSLTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGVKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATA 430          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: ben-1 (Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:Q18817])

HSP 1 Score: 812.372 bits (2097), Expect = 0.000e+0
Identity = 397/430 (92.33%), Postives = 418/430 (97.21%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIG+KFWEVISDEHGI P G+Y G+SDLQLERINVYYNEA GGKYVPRAVL+DLEPG MDSVRSGPFGQLFRPDNF+FGQSGAGNNWAKGHYTEGAELVD+VLD VRKEAE CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM++FSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKL+ PTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPL+++G+Q YRALTV ELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSM+EVD+QM+NVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 
Sbjct:    1 MREIVHVQAGQCGNQIGAKFWEVISDEHGIQPDGTYKGESDLQLERINVYYNEANGGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLSNPTYGDLNHLVSVTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGAQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDDQMMNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATA 430          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: mec-7 (Tubulin beta-1 chain [Source:UniProtKB/Swiss-Prot;Acc:P12456])

HSP 1 Score: 808.52 bits (2087), Expect = 0.000e+0
Identity = 399/426 (93.66%), Postives = 413/426 (96.95%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 1376
            MREIVH+QAGQCGNQIGSKFWEVISDEHGIDP+G Y GDSDLQLERINVYYNEA   KYVPRAVL+DLEPG MDSVRSGPFGQLFRPDN++FGQSGAGNNWAKGHYTEGAELVD+VLD VRKEAES DCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTD TFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSR +QQYRA+TVPELTQQ FDAKNMMAACDPRHGRYLT AA+FRGRMSMKEVDEQMLN+QNKNSSYFV+WIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ
Sbjct:    1 MREIVHIQAGQCGNQIGSKFWEVISDEHGIDPSGQYVGDSDLQLERINVYYNEAGSNKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNYVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAESTDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDSTFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRSNQQYRAITVPELTQQCFDAKNMMAACDPRHGRYLTAAAIFRGRMSMKEVDEQMLNIQNKNSSYFVDWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 426          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-2 (Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P52275])

HSP 1 Score: 805.823 bits (2080), Expect = 0.000e+0
Identity = 391/430 (90.93%), Postives = 417/430 (96.98%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIGSKFWEVISDEHGI P G++ G++DLQLERI+VYYNEA  GKYVPRAVL+DLEPG MDSVRSGPFGQLFRPDNF+FGQSGAGNNWAKGHYTEGAELVD+VLD +RKEAE CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM++FSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDIC+RTLKLT PTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPL+++G+Q YRALTV ELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSM+EVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATF+GNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDL+SEYQQYQEAT 
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQPDGTFKGETDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICYRTLKLTNPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGTQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLISEYQQYQEATA 430          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-2 (Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P52275])

HSP 1 Score: 805.823 bits (2080), Expect = 0.000e+0
Identity = 391/430 (90.93%), Postives = 417/430 (96.98%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIGSKFWEVISDEHGI P G++ G++DLQLERI+VYYNEA  GKYVPRAVL+DLEPG MDSVRSGPFGQLFRPDNF+FGQSGAGNNWAKGHYTEGAELVD+VLD +RKEAE CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM++FSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDIC+RTLKLT PTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPL+++G+Q YRALTV ELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSM+EVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATF+GNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDL+SEYQQYQEAT 
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQPDGTFKGETDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICYRTLKLTNPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGTQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLISEYQQYQEATA 430          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub56D (gene:FBgn0284243 transcript:FBtr0086536)

HSP 1 Score: 835.484 bits (2157), Expect = 0.000e+0
Identity = 409/429 (95.34%), Postives = 424/429 (98.83%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVH+QAGQCGNQIG+KFWE+ISDEHGID TG+YHGDSDLQLERINVYYNEA+GGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM T+SVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLN+QNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDATGAYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 429          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub85D (gene:FBgn0003889 transcript:FBtr0082046)

HSP 1 Score: 822.772 bits (2124), Expect = 0.000e+0
Identity = 406/430 (94.42%), Postives = 419/430 (97.44%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIG KFWEVISDEH ID TG+Y+GDSDLQLERINVYYNEATG KYVPRA+L+DLEPG MDSVRSG FGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKE+E CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLN+QNKNSS+FVEWIPNN KTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 
Sbjct:    1 MREIVHIQAGQCGNQIGGKFWEVISDEHCIDATGTYYGDSDLQLERINVYYNEATGAKYVPRAILVDLEPGTMDSVRSGAFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSFFVEWIPNNCKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATA 430          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub56D (gene:FBgn0284243 transcript:FBtr0086537)

HSP 1 Score: 795.038 bits (2052), Expect = 0.000e+0
Identity = 392/410 (95.61%), Postives = 405/410 (98.78%), Query Frame = 3
Query:  156 FWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            FWE+ISDEHGID TG+YHGDSDLQLERINVYYNEA+GGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM T+SVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLN+QNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT
Sbjct:   29 FWEIISDEHGIDATGAYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 438          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub60D (gene:FBgn0003888 transcript:FBtr0072270)

HSP 1 Score: 793.497 bits (2048), Expect = 0.000e+0
Identity = 389/435 (89.43%), Postives = 418/435 (96.09%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAT------GGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIV+LQAGQCGNQIG+KFWE+IS+EHGID  G Y GDSDLQLER++VYYNEA+      GGKYVPRA+L+DLEPG M+SVRSGP+GQLFRPDNF++GQSGAGNNWAKGHYTEGAELVD+VLD VRKE E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM T+SVVPSPKVSDTVVEPYNATLS+HQLVENTDET+CIDNEALYDICFRTLK++ P+YGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQML VQNKNSSYFVEWIPNN+KTAVCDIPP+GLKMS+TFIGN+TAIQELFKR+SEQF+AMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT
Sbjct:    1 MREIVNLQAGQCGNQIGAKFWEIISEEHGIDSNGIYVGDSDLQLERVSVYYNEASAVTRSSGGKYVPRAILLDLEPGTMESVRSGPYGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELVDNVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKVSNPSYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLAVQNKNSSYFVEWIPNNVKTAVCDIPPKGLKMSSTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 435          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub60D (gene:FBgn0003888 transcript:FBtr0343443)

HSP 1 Score: 793.112 bits (2047), Expect = 0.000e+0
Identity = 389/434 (89.63%), Postives = 418/434 (96.31%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAT-----GGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIV+LQAGQCGNQIG+KFWE+IS+EHGID  G Y GDSDLQLER++VYYNEA+     GGKYVPRA+L+DLEPG M+SVRSGP+GQLFRPDNF++GQSGAGNNWAKGHYTEGAELVD+VLD VRKE E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM T+SVVPSPKVSDTVVEPYNATLS+HQLVENTDET+CIDNEALYDICFRTLK++ P+YGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQML VQNKNSSYFVEWIPNN+KTAVCDIPP+GLKMS+TFIGN+TAIQELFKR+SEQF+AMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT
Sbjct:    1 MREIVNLQAGQCGNQIGAKFWEIISEEHGIDSNGIYVGDSDLQLERVSVYYNEASVTRSSGGKYVPRAILLDLEPGTMESVRSGPYGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELVDNVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKVSNPSYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLAVQNKNSSYFVEWIPNNVKTAVCDIPPKGLKMSSTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 434          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: tubb4b (tubulin, beta 4B class IVb [Source:ZFIN;Acc:ZDB-GENE-030131-8625])

HSP 1 Score: 842.417 bits (2175), Expect = 0.000e+0
Identity = 414/429 (96.50%), Postives = 426/429 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGSYHGDSDLQL+RINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLDRINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 842.032 bits (2174), Expect = 0.000e+0
Identity = 414/429 (96.50%), Postives = 426/429 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 842.032 bits (2174), Expect = 0.000e+0
Identity = 414/429 (96.50%), Postives = 426/429 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 842.032 bits (2174), Expect = 0.000e+0
Identity = 414/429 (96.50%), Postives = 426/429 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 841.647 bits (2173), Expect = 0.000e+0
Identity = 414/429 (96.50%), Postives = 426/429 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: reps2 (RALBP1 associated Eps domain containing 2 [Source:Xenbase;Acc:XB-GENE-985000])

HSP 1 Score: 843.188 bits (2177), Expect = 0.000e+0
Identity = 415/429 (96.74%), Postives = 426/429 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: znf330 (zinc finger protein 330 [Source:Xenbase;Acc:XB-GENE-1010548])

HSP 1 Score: 837.025 bits (2161), Expect = 0.000e+0
Identity = 410/430 (95.35%), Postives = 425/430 (98.84%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATG K+VPRA+L+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSV+PSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: tubb (tubulin beta class I [Source:NCBI gene;Acc:448742])

HSP 1 Score: 833.943 bits (2153), Expect = 0.000e+0
Identity = 408/429 (95.10%), Postives = 425/429 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQL+RI+VYYNEATGGKYVPRA+L+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: ubxn11 (UBX domain protein 11 [Source:Xenbase;Acc:XB-GENE-985480])

HSP 1 Score: 833.173 bits (2151), Expect = 0.000e+0
Identity = 407/427 (95.32%), Postives = 424/427 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 1379
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEA+GGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSV+D VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYR+LTVPELTQQMFD+KNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVMDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRSLTVPELTQQMFDSKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: tubb3 (tubulin beta 3 class III [Source:NCBI gene;Acc:395045])

HSP 1 Score: 803.127 bits (2073), Expect = 0.000e+0
Identity = 394/430 (91.63%), Postives = 414/430 (96.28%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDP G+Y GDSDLQLERI+VYYNEA+  KYVPRA+L+DLEPG MDSVRSG FG LFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLD VRKE E+CDCLQGFQLTHSLGGGTGSGMGTLLISK+REEYPDRIM TFSVVPSPKVSDTVVEPYNATLS+HQLVENTDET+CIDNEALYDICFRTLKL TPTYGDLNHLVSATMSGVTT LRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLT+RGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVA +FRGRMSMKEVDEQML +Q+KNSSYFVEWIPNN+K AVCDIPPRGLKMS+TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPCGNYIGDSDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRGLKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb4b (tubulin, beta 4B class IVB [Source:MGI Symbol;Acc:MGI:1915472])

HSP 1 Score: 839.721 bits (2168), Expect = 0.000e+0
Identity = 413/427 (96.72%), Postives = 424/427 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 1379
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb2b (tubulin, beta 2B class IIB [Source:MGI Symbol;Acc:MGI:1920960])

HSP 1 Score: 837.795 bits (2163), Expect = 0.000e+0
Identity = 410/430 (95.35%), Postives = 425/430 (98.84%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATG KYVPRA+L+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSV+PSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb5 (tubulin, beta 5 class I [Source:MGI Symbol;Acc:MGI:107812])

HSP 1 Score: 833.943 bits (2153), Expect = 0.000e+0
Identity = 408/429 (95.10%), Postives = 425/429 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQL+RI+VYYNEATGGKYVPRA+L+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb2a (tubulin, beta 2A class IIA [Source:MGI Symbol;Acc:MGI:107861])

HSP 1 Score: 832.787 bits (2150), Expect = 0.000e+0
Identity = 408/430 (94.88%), Postives = 423/430 (98.37%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEA G KYVPRA+L+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSV+PSPKVSDTVVEPYNATLSVHQLVENTDET+ IDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb4a (tubulin, beta 4A class IVA [Source:MGI Symbol;Acc:MGI:107848])

HSP 1 Score: 829.706 bits (2142), Expect = 0.000e+0
Identity = 407/427 (95.32%), Postives = 423/427 (99.06%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 1379
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGG YVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVD+VLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREE+PDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQML+VQ+KNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P30883|TBB4_XENLA (Tubulin beta-4 chain OS=Xenopus laevis OX=8355 GN=tubb4 PE=2 SV=1)

HSP 1 Score: 843.958 bits (2179), Expect = 0.000e+0
Identity = 415/430 (96.51%), Postives = 426/430 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGAYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P11833|TBB_PARLI (Tubulin beta chain OS=Paracentrotus lividus OX=7656 PE=2 SV=1)

HSP 1 Score: 842.803 bits (2176), Expect = 0.000e+0
Identity = 414/430 (96.28%), Postives = 426/430 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:    1 MREIVHMQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P68372|TBB4B_MOUSE (Tubulin beta-4B chain OS=Mus musculus OX=10090 GN=Tubb4b PE=1 SV=1)

HSP 1 Score: 839.721 bits (2168), Expect = 0.000e+0
Identity = 413/427 (96.72%), Postives = 424/427 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 1379
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P68371|TBB4B_HUMAN (Tubulin beta-4B chain OS=Homo sapiens OX=9606 GN=TUBB4B PE=1 SV=1)

HSP 1 Score: 839.721 bits (2168), Expect = 0.000e+0
Identity = 413/427 (96.72%), Postives = 424/427 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 1379
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|Q3MHM5|TBB4B_BOVIN (Tubulin beta-4B chain OS=Bos taurus OX=9913 GN=TUBB4B PE=2 SV=1)

HSP 1 Score: 839.721 bits (2168), Expect = 0.000e+0
Identity = 413/427 (96.72%), Postives = 424/427 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 1379
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A2U9BBA2 (Ras GTPase-activating-like protein IQGAP1 OS=Scophthalmus maximus OX=52904 GN=SMAX5B_007995 PE=3 SV=1)

HSP 1 Score: 855.129 bits (2208), Expect = 0.000e+0
Identity = 410/429 (95.57%), Postives = 426/429 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGSYHGDSDLQL+RINVYYNEA+GGKYVPRAVL+DLEPG MD+VRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTP+YGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYR+LTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLDRINVYYNEASGGKYVPRAVLVDLEPGTMDAVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPSYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRSLTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A369S2L5 (Tubulin beta chain OS=Trichoplax sp. H2 OX=287889 GN=TrispH2_006238 PE=3 SV=1)

HSP 1 Score: 847.425 bits (2188), Expect = 0.000e+0
Identity = 416/436 (95.41%), Postives = 429/436 (98.39%), Query Frame = 3
Query:   78 LNLFKNKMREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            L   K KMREIVHLQAGQCGNQIG+KFWEVISDEHG+DPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMS+TF+GNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:   16 LQFLKVKMREIVHLQAGQCGNQIGAKFWEVISDEHGVDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSSTFVGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 451          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A1I8FZR8 (Tubulin beta chain OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 847.425 bits (2188), Expect = 0.000e+0
Identity = 415/430 (96.51%), Postives = 426/430 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMS+TFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:   61 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSSTFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 490          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A5S6QE49 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1)

HSP 1 Score: 847.04 bits (2187), Expect = 0.000e+0
Identity = 413/430 (96.05%), Postives = 426/430 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEA+GGKYVPRA+L+DLEPG MDSVR+GPFG LFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLD VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQ YRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNS+YFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRAGPFGTLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQGYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSAYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 430          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A267E855 (Tubulin beta chain (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021796g2 PE=3 SV=1)

HSP 1 Score: 846.655 bits (2186), Expect = 0.000e+0
Identity = 415/430 (96.51%), Postives = 427/430 (99.30%), Query Frame = 3
Query:   96 KMREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            +MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMS+TFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:   33 RMREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSSTFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 462          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: zgc:55461 (tubulin beta-4B chain [Source:NCBI gene;Acc:103024068])

HSP 1 Score: 842.032 bits (2174), Expect = 0.000e+0
Identity = 414/429 (96.50%), Postives = 426/429 (99.30%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGAYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: TUBB2A (tubulin beta-2A chain [Source:NCBI gene;Acc:103041736])

HSP 1 Score: 826.239 bits (2133), Expect = 0.000e+0
Identity = 403/433 (93.07%), Postives = 424/433 (97.92%), Query Frame = 3
Query:   90 KNKMREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            +N MREIVH+QAGQCGNQIG+KFWEVISDEHGID TGSY G+S+LQLERINVYYNEATG K+VPRA+L+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKE+E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSV+PSPKVSDTVVEPYNATLSVHQLVENTDETF IDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRAL+VPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQML+VQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELF+R+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:   75 QNNMREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGSYQGESELQLERINVYYNEATGSKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETFSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLSVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFRRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 507          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: TUBB1 (tubulin beta 1 class VI [Source:HGNC Symbol;Acc:HGNC:16257])

HSP 1 Score: 683.33 bits (1762), Expect = 0.000e+0
Identity = 341/429 (79.49%), Postives = 371/429 (86.48%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVHLQ GQCGNQIGSK  E+      ID                        GGKYVPRA+L+DLEPG MDSVR    GQLFRPDNFI G SGAGNNWAKGHYTEGAELV+ V+D VR EAESCDCLQGFQ  HSLGGGTGSGMGTL+I+KIREEYPDRIM +FS++PSPKVSDTVVEPYNATLS+HQL+ENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVS TMSGVTT LRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLT+RGSQQYRALTVPELTQQMFDA+NMM ACDPR GRYLTVA +FRGRMS KEVDEQML +Q KNS+YFV+WIP+N+K AVCDIPPRGLKM++TFIGN+TAIQE+F+RV EQF  MFRRKAFLHWYTGEGMDEMEFTEAE+N+NDLVSEYQQYQ+AT
Sbjct:    1 MREIVHLQIGQCGNQIGSKVTEI----RLID----------------------TCGGKYVPRALLVDLEPGTMDSVRGSRIGQLFRPDNFIHGNSGAGNNWAKGHYTEGAELVEQVVDRVRNEAESCDCLQGFQFVHSLGGGTGSGMGTLIINKIREEYPDRIMNSFSIMPSPKVSDTVVEPYNATLSIHQLIENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDARNMMTACDPRRGRYLTVAGIFRGRMSTKEVDEQMLAIQQKNSNYFVDWIPHNVKVAVCDIPPRGLKMASTFIGNNTAIQEIFRRVGEQFAMMFRRKAFLHWYTGEGMDEMEFTEAENNLNDLVSEYQQYQDAT 403          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: ENSAMXT00000019550.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000026.1:1405913:1407004:-1 gene:ENSAMXG00000018987.2 transcript:ENSAMXT00000019550.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 644.81 bits (1662), Expect = 0.000e+0
Identity = 323/335 (96.42%), Postives = 332/335 (99.10%), Query Frame = 3
Query:  375 GQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 1379
            GQSGAGNNWAKGHYTEGAELVDSVLD VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:   12 GQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 346          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: ENSAMXT00000030346.1 (tubulin alpha-1B chain [Source:NCBI gene;Acc:111195381])

HSP 1 Score: 358.992 bits (920), Expect = 4.777e-116
Identity = 181/434 (41.71%), Postives = 267/434 (61.52%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQL--ERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPR--------GLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 1370
            MRE + +  GQ G QIG+  WE+   EHGI P G    D  +    +  N +++E   GK+VPRAV +DLEP ++D VR+G + QLF P+  I G+  A NN+A+GHYT G E++D VLD +RK A+ C  LQGF + HS GGGTGSG  +LL+ ++  +Y  +    FS+ P+P+VS  VVEPYN+ L+ H  +E++D  F +DNEA+YDIC R L +  PTY +LN L+S  +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPRHG+Y+    L+RG +  K+V+  +  ++ K +  FV+W P   K  +   PP          ++ +   + N+TAI E + R+  +F  M+ ++AF+HWY GEGM+E EF+EA  +M  L  +Y++
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEE 434          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: tubb2 (tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GENE-050522-384])

HSP 1 Score: 701.434 bits (1809), Expect = 0.000e+0
Identity = 350/375 (93.33%), Postives = 366/375 (97.60%), Query Frame = 3
Query:  153 KFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM-TTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRK 1274
            +FWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEA+GGKYVPRA+L+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEY  +++ T  +  PSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRK
Sbjct:    1 QFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYIIQVLYTVVASGPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRK 375          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: TUBA3C (tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:12408])

HSP 1 Score: 300.056 bits (767), Expect = 1.799e-95
Identity = 152/348 (43.68%), Postives = 219/348 (62.93%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQL--ERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIP 1136
            MRE + +  GQ G QIG+  WE+   EHGI P G    D  +    +  N +++E   GK+VPRAV +DLEP ++D VR+G + QLF P+  I G+  A NN+A+GHYT G E+VD VLD +RK A+ C  LQGF + HS GGGTGSG  +LL+ ++  +Y  +    F++ P+P+VS  VVEPYN+ L+ H  +E++D  F +DNEA+YDIC R L +  PTY +LN L+   +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPRHG+Y+    L+RG +  K+V+  +  ++ K +  FV+W P
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCP 348          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009623.1 (pep scaffold:Pmarinus_7.0:GL485233:4234:10583:1 gene:ENSPMAG00000008696.1 transcript:ENSPMAT00000009623.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 249.98 bits (637), Expect = 3.659e-79
Identity = 135/159 (84.91%), Postives = 150/159 (94.34%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHG-DSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREE 572
            MREIVH+QAGQCGNQ+GSKFWEVIS+EHGID TGSY G ++ LQ+ERINVYY+EAT GK+VPRA+++DLEPG MD+VRSGP G +FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAE+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREE
Sbjct:    1 MREIVHVQAGQCGNQVGSKFWEVISEEHGIDRTGSYRGYNARLQMERINVYYSEATSGKFVPRAIMVDLEPGTMDAVRSGPLGHMFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREE 159          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: tubg1 (tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-836])

HSP 1 Score: 256.914 bits (655), Expect = 6.821e-78
Identity = 143/439 (32.57%), Postives = 239/439 (54.44%), Query Frame = 3
Query:  102 REIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQ--SGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSP-KVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGS-QQYRALTVPELTQQMFDAKNMMAACDPRHGR-------YLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPP---RGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGM---DEMEFTEAESNMNDLVSEYQ 1367
            REI+ LQ GQCGNQIG +FW+ +  EHGI P G     +    +R +V++ +A    Y+PRAVL+DLEP +++S+ +  +  L+ P+N    +   GAGNNWA GH ++G ++ + + D + +EA+  D L+             S      + K    +P +++ T+SV P+  ++SD VV+PYN+ L++ +L +N D    +DN AL  I    L +  P++  +N LVS  MS  TT LR+PG +N DL  L  +++P PRLHF + G+ PLT+  S    R  TV ++ +++   KN+M +     GR       Y+ +  + +G +   +V + +  ++ +  + F+ W P +I+ A+    P      ++S   + N T+I  LF R  +Q+  + +R+AFL  +  E M   D  EF ++   +  L+ EYQ
Sbjct:    3 REIITLQLGQCGNQIGFEFWKQLCAEHGISPDGILEDFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVINSIVNSSYANLYNPENIYLSEHGGGAGNNWASGH-SQGEKIHEEIFDIIDREADGSDSLE-----KCPLTRRLSATNWNELCKYFCRFPKKLIQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVST----GRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLTSAHRVSGLMMANHTSISSLFDRTCKQYDKLRKREAFLDQFRKEDMFKEDLSEFDDSREVVQQLIDEYQ 431          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001139.1 (pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 gene:ENSPMAG00000001018.1 transcript:ENSPMAT00000001139.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 234.187 bits (596), Expect = 1.858e-70
Identity = 129/352 (36.65%), Postives = 202/352 (57.39%), Query Frame = 3
Query:  102 REIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQL---ERINVYYNEATGGKYVPRAVLIDLEPGIMD---SVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPN 1139
            R+ + +  GQ G Q+G+  WE+   EHGI P G      D+        N +++E   GK+VPRA+ IDLEP ++     +R+   G   R    + G+    +   +G    G ++   VL   R ++E C  LQGF L HS GGGTGSG  +LL+ ++  +Y  +    F + P+P+VS  VVEPYNA L+ H  +E++D +F +DNEA+YDIC + L +  P+Y +LN L++  +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPR+G+Y+    L+RG +  K+V+  +  ++++ +  FV+W P 
Sbjct:    3 RDCISIHIGQAGVQMGNACWELYCLEHGIKPDGHLATHPDVTSVVDSSFNTFFSETGSGKHVPRAIFIDLEPSVIGEPCGLRTLTVGGXLRMSMMLAGREVRSSRIWRGQKGGGRKICSRVLRRNRPQSERCTGLQGFLLFHSFGGGTGSGFTSLLMERLSADYGKKSKLEFCIYPAPQVSTAVVEPYNAILTTHTTLEHSDCSFMVDNEAIYDICRKKLDIDFPSYFNLNRLIAQMVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLTTYAPVISAEKAYHEQLSVAEITNACFEPSNQMVKCDPRNGKYMACCLLYRGDIVPKDVNTAIAIIKSRRAVRFVDWCPT 354          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB2 (Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria [Source:SGD;Acc:S000001857])

HSP 1 Score: 686.411 bits (1770), Expect = 0.000e+0
Identity = 330/430 (76.74%), Postives = 386/430 (89.77%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREI+H+  GQCGNQIG+ FWE I  EHG+D  G+YHG  D+Q ER+NVY+NEA+ GK+VPR++ +DLEPG +D+VR+   G LFRPDN+IFGQS AGN WAKGHYTEGAELVDSV+D +R+EAE CD LQGFQ+THSLGGGTGSGMGTLLISKIREE+PDR+M TFSV+PSPK SDTVVEPYNATLSVHQLVE++DETFCIDNEALYDIC RTLKL  P+YGDLN+LVS+ MSGVTT LR+PGQLN+DLRKLAVN+VPFPRLHFF+ G+APLT+ GSQ +R+LTVPELTQQMFDAKNMMAA DPR+GRYLTVAA FRG++S+KEV+++M  VQ+KNS YFVEWIPNN++TAVC + P+GL M+ATFI NST+IQELFKRV +QF+AMF+RKAFLHWYT EGMDE+EF+EAESNMNDLVSEYQQYQEATV
Sbjct:    1 MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYHGHDDIQKERLNVYFNEASSGKWVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTEGAELVDSVMDVIRREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVLPSPKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGSQSFRSLTVPELTQQMFDAKNMMAAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSDYFVEWIPNNVQTAVCSVAPQGLDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWYTSEGMDELEFSEAESNMNDLVSEYQQYQEATV 430          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB1 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication [Source:SGD;Acc:S000004550])

HSP 1 Score: 352.443 bits (903), Expect = 1.705e-114
Identity = 182/435 (41.84%), Postives = 272/435 (62.53%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQL-----ERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRG--------LKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEY 1364
            MRE++ +  GQ G QIG+  WE+ S EHGI P G  H +  L       E  + +++E   GK+VPRA+ +DLEP ++D VR+GP+  LF P+  I G+  A NN+A+GHYT G E++  VLD +RK A+ CD LQGF  THSLGGGTGSG+G+LL+ ++  EY  +    F+V P+P+VS +VVEPYN  L+ H  +E+ D TF +DNEA+YD+C R L +  P++ +LN+L++  +S VT  LRF G LN DL +   N+VP+PR+HF +  ++P+ S+    + + +V E+T   F+  N M  CDPR G+Y+    L+RG +  ++V   +  V+NK +   V+W P   K  +C  PP          +  +   + N+T+I E +KR+  +F  M+ ++AF+HWY GEGM+E EFTEA  ++  L  +Y
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKPDG--HLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRNGPYKDLFHPEQLISGKEDAANNYARGHYTVGREILGDVLDRIRKLADQCDGLQGFLFTHSLGGGTGSGLGSLLLEELSAEYGKKSKLEFAVYPAPQVSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDMCKRNLDIPRPSFANLNNLIAQVVSSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYSPVLSKSKAFHESNSVSEITNACFEPGNQMVKCDPRDGKYMATCLLYRGDVVTRDVQRAVEQVKNKKTVQLVDWCPTGFKIGICYEPPTATPNSQLATVDRAVCMLSNTTSIAEAWKRIDRKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDY 433          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB3 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication [Source:SGD;Acc:S000004593])

HSP 1 Score: 347.051 bits (889), Expect = 2.260e-112
Identity = 182/435 (41.84%), Postives = 270/435 (62.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQL-----ERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMS--------ATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEY 1364
            MRE++ +  GQ G QIG+  WE+ S EHGI   G  H +  L       E  + +++E   GK+VPRA+ +DLEP ++D VR+G F +LF P+  I G+  A NN+A+GHYT G E+VD V + +RK A+ CD LQGF  THSLGGGTGSG+G+LL+  +  EY  +    F+V P+P++S +VVEPYN  L+ H  +E+ D TF +DNEA+YDIC R L ++ P++ +LN L++  +S VT  LRF G LN DL +   N+VP+PR+HF +  +AP+ S+    + + +V E+T   F+  N M  CDP  G+Y+    L+RG +  ++V   +  V+NK +   V+W P   K  +C  PP  +  S           + N+TAI + +KR+ ++F  M+ ++AF+HWY GEGM+E EFTEA  ++  L  +Y
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKEDG--HLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRTGRFKELFHPEQLINGKEDAANNYARGHYTVGREIVDEVEERIRKMADQCDGLQGFLFTHSLGGGTGSGLGSLLLENLSYEYGKKSKLEFAVYPAPQLSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDICKRNLGISRPSFSNLNGLIAQVISSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPILSKKRATHESNSVSEITNACFEPGNQMVKCDPTKGKYMANCLLYRGDVVTRDVQRAVEQVKNKKTVQMVDWCPTGFKIGICYEPPSVIPSSELANVDRAVCMLSNTTAIADAWKRIDQKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDY 433          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB4 (Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004202])

HSP 1 Score: 174.481 bits (441), Expect = 9.986e-48
Identity = 130/444 (29.28%), Postives = 217/444 (48.87%), Query Frame = 3
Query:  105 EIVHLQAGQCGNQIGSKFWEVISDEHGID-------PTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGA--GNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTS---RGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPN----NI--KTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWY-TGEGMDEM-----EFTEAESNMNDLVSEY 1364
            EI+ LQAGQCGN +G   W  ++ EH I        P  S   D D +      ++ E +  K+ PRA+++D EP ++  V +  F   F P N      GA  GN+WA G Y  G    D +L+ + KE +S D  +GFQL HS+ GGTGSG+G+ L+  + + YP +I+TT+SV P+ + S+ VV+ YN  L++ +L+E++D T   DN +L +I  +  +         N L+S  +S VT  +RFP  + + +  +   ++P P LHF  P F P TS        ++  +  ++   + D  N + +    +  Y  V     G +  +++   M  +Q +    F  W  +    NI  ++    + P   ++S   + N + +  +F+     F  +F + AFL+ Y  G+    M     EF E+   +  L+ +Y
Sbjct:    4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSSTERDDDTK-----PFFRENSRNKFTPRAIMMDSEPSVIADVEN-TFRGFFDPRNTWVASDGASAGNSWANG-YDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDRYPKKILTTYSVFPA-RSSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQLISTIISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGHSSYDVMLDLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTKLQQR--IKFPSWSSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQSMQNVQDEFAESREVVQSLMEDY 437          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO39945 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8I3])

HSP 1 Score: 840.877 bits (2171), Expect = 0.000e+0
Identity = 413/430 (96.05%), Postives = 425/430 (98.84%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRA+L+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:    1 MREIVHCQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO35958 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJX9])

HSP 1 Score: 837.025 bits (2161), Expect = 0.000e+0
Identity = 411/429 (95.80%), Postives = 425/429 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIV LQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPR+VLIDLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAEL+DSVLD VRKEAESCDC+QGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFS+VPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVSLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRSVLIDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELMDSVLDVVRKEAESCDCIQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSIVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO42890 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S027])

HSP 1 Score: 820.076 bits (2117), Expect = 0.000e+0
Identity = 404/429 (94.17%), Postives = 418/429 (97.44%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVHLQAGQCGNQIG+KFW    +EHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDC+QGFQLTHSLGGGTGSGM TLLI KIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRL FF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDP HGRYLTVAA+FRGRMSMKE+DEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHLQAGQCGNQIGAKFW----NEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCIQGFQLTHSLGGGTGSGMVTLLILKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLPFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPLHGRYLTVAAMFRGRMSMKELDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 425          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO49518 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGG3])

HSP 1 Score: 681.019 bits (1756), Expect = 0.000e+0
Identity = 324/430 (75.35%), Postives = 377/430 (87.67%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTG--SYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEA 1382
            MREI+++Q GQCGNQIG++FW  I++EHGIDPTG  S   D+  + +RI+VY++E  GG+YVPR++L+DLEPG MD+VR GP G +FRPDNF+  QSGA NNWAKGHY+EGAEL+D++ D +RKEAE+CDC+QG QL  +LGGGTGSG+GTL++SKIREEYPDRI++ FSVVPSPKVS+TVVEPYNATLSVHQLVE  DE F  DNEALYDIC RTLKL  P Y DLN LVS  M G+T  LRFPGQLN DLRKLAVNMVPFPRLHFF+PGFAPL SR +QQY+ ++V ELTQQMFDA+NMM ACDPRHGRYLTVAA+FRG+MSM+EVDEQMLN QNKNS +FVEWIPNN+KT VCDI P+GLKM+ATF+GN+TAIQELFKRV EQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNM+DL++EYQQYQEA
Sbjct:    1 MREIINIQVGQCGNQIGTEFWRDIAEEHGIDPTGAKSLIEDTHGRFDRIDVYFHEGNGGRYVPRSILVDLEPGTMDAVRGGPLGNMFRPDNFVNSQSGAANNWAKGHYSEGAELIDNIFDVLRKEAENCDCMQGVQLIQALGGGTGSGLGTLILSKIREEYPDRILSAFSVVPSPKVSETVVEPYNATLSVHQLVEVVDEDFMFDNEALYDICCRTLKLAKPNYADLNQLVSMAMCGLTASLRFPGQLNGDLRKLAVNMVPFPRLHFFMPGFAPLASRANQQYQNVSVEELTQQMFDARNMMTACDPRHGRYLTVAAIFRGKMSMREVDEQMLNTQNKNSPFFVEWIPNNVKTTVCDIAPKGLKMAATFVGNNTAIQELFKRVGEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMHDLIAEYQQYQEA 430          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO36734 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHY4])

HSP 1 Score: 655.596 bits (1690), Expect = 0.000e+0
Identity = 329/339 (97.05%), Postives = 336/339 (99.12%), Query Frame = 3
Query:  369 IFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            + GQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MLGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 339          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tubb2 (tubulin beta-2B chain [Source:NCBI gene;Acc:101162245])

HSP 1 Score: 830.091 bits (2143), Expect = 0.000e+0
Identity = 406/430 (94.42%), Postives = 424/430 (98.60%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGSY GDSDLQLERINVYYNEA+G KYVPRA+L+DLEPG MDSVRSGPFGQLFRPDNF+FGQSGAGNNWAKGHYTEGAELVD+VLD VRKE+E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSV+PSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRAL+VPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSM+EVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELF+R+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYQGDSDLQLERINVYYNEASGSKYVPRAILVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFRRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tubb5 (tubulin beta chain [Source:NCBI gene;Acc:101157969])

HSP 1 Score: 828.55 bits (2139), Expect = 0.000e+0
Identity = 405/427 (94.85%), Postives = 423/427 (99.06%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 1379
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQL+RI+VYYNEATGGKYVPRA+L+DLEPG MDSVRSGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000045809.1 (tubulin beta-6 chain [Source:NCBI gene;Acc:101166121])

HSP 1 Score: 741.11 bits (1912), Expect = 0.000e+0
Identity = 358/429 (83.45%), Postives = 395/429 (92.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 1385
            MREIVH+Q GQCGNQIGSKFWEVIS+EHGI+ TG Y GDS LQLERINVY++EA GGKYVPRA+L+DLEPG MDS+R    G LFRPDNFI G SGAGNNWAKGHYTEGAELV+ V+D VR E+E CDCLQGFQL HSLGGGTGSGMGTL+I+KI+EE+PDRIM TFS++PSPKVSDTVVEPYNATLSVHQL+EN D TFCIDNEALYDICFRTLKLTTPTYGDLNHLV  TMSGVTT LRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLT+RGSQQYRALTVPELTQQMFDA+NMM ACDPR GRYLTVA +FRG+MS KEVDEQML +Q KNS+YFV+WIP+N+K AVCDIPP+GL+M++TFIGN+TAIQE+F+RV EQF+ MFRRKAFLHWYTGEGMDEMEFTEAESN+NDLVSEYQQYQEAT
Sbjct:    1 MREIVHVQIGQCGNQIGSKFWEVISEEHGINATGIYEGDSTLQLERINVYFSEAYGGKYVPRALLVDLEPGTMDSIRGSRIGALFRPDNFIHGNSGAGNNWAKGHYTEGAELVEQVVDRVRHESEGCDCLQGFQLVHSLGGGTGSGMGTLIINKIKEEFPDRIMNTFSIMPSPKVSDTVVEPYNATLSVHQLLENADATFCIDNEALYDICFRTLKLTTPTYGDLNHLVCMTMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDARNMMTACDPRRGRYLTVAGIFRGKMSTKEVDEQMLAIQQKNSTYFVDWIPHNVKVAVCDIPPKGLRMASTFIGNNTAIQEIFRRVGEQFSLMFRRKAFLHWYTGEGMDEMEFTEAESNLNDLVSEYQQYQEAT 429          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000033163.1 (tubulin alpha-1A chain [Source:NCBI gene;Acc:105354455])

HSP 1 Score: 361.303 bits (926), Expect = 6.169e-117
Identity = 183/434 (42.17%), Postives = 267/434 (61.52%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQL--ERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPR--------GLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 1370
            MRE + +  GQ G QIG+  WE+   EHGI P G    D  +    +  N +++E   GK+VPRAV +DLEP ++D VR+G + QLF P+  I G+  A NN+A+GHYT G E++D VLD +RK A+ C  LQGF + HS GGGTGSG  +LL+ ++  +Y  +    FS+ P+P+VS  VVEPYNA L+ H  +E++D  F +DNEA+YDIC R L +  PTY +LN L+S  +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPRHG+Y+    L+RG +  K+V+  +  ++ K S  FV+W P   K  +   PP          ++ +   + N+TAI E + R+  +F  M+ ++AF+HWY GEGM+E EF+EA  +M  L  +Y++
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEE 434          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000020578.2 (tubulin alpha-1A chain [Source:NCBI gene;Acc:105354455])

HSP 1 Score: 361.303 bits (926), Expect = 6.169e-117
Identity = 183/434 (42.17%), Postives = 267/434 (61.52%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQL--ERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPR--------GLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 1370
            MRE + +  GQ G QIG+  WE+   EHGI P G    D  +    +  N +++E   GK+VPRAV +DLEP ++D VR+G + QLF P+  I G+  A NN+A+GHYT G E++D VLD +RK A+ C  LQGF + HS GGGTGSG  +LL+ ++  +Y  +    FS+ P+P+VS  VVEPYNA L+ H  +E++D  F +DNEA+YDIC R L +  PTY +LN L+S  +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPRHG+Y+    L+RG +  K+V+  +  ++ K S  FV+W P   K  +   PP          ++ +   + N+TAI E + R+  +F  M+ ++AF+HWY GEGM+E EF+EA  +M  L  +Y++
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEE 434          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000044907.1 (SMESG000044907.1)

HSP 1 Score: 854.358 bits (2206), Expect = 0.000e+0
Identity = 424/430 (98.60%), Postives = 429/430 (99.77%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREI+HLQAGQCGNQIG+KFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVD+QMLNVQNKNSSYFVEWIPNN+KTAVCDI PRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV
Sbjct:    1 MREILHLQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDDQMLNVQNKNSSYFVEWIPNNVKTAVCDIAPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 430          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000047291.1 (SMESG000047291.1)

HSP 1 Score: 848.195 bits (2190), Expect = 0.000e+0
Identity = 421/430 (97.91%), Postives = 426/430 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTG+YHGDSDLQLERINVY+NEATGGKYVPRAVLIDLEPG MDSVRSGPFGQLFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000044888.1 (SMESG000044888.1)

HSP 1 Score: 848.195 bits (2190), Expect = 0.000e+0
Identity = 421/430 (97.91%), Postives = 426/430 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTG+YHGDSDLQLERINVY+NEATGGKYVPRAVLIDLEPG MDSVRSGPFGQLFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000047284.1 (SMESG000047284.1)

HSP 1 Score: 846.655 bits (2186), Expect = 0.000e+0
Identity = 419/430 (97.44%), Postives = 426/430 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIGSKFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVR+GPFGQLFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:    1 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000046374.1 (SMESG000046374.1)

HSP 1 Score: 846.655 bits (2186), Expect = 0.000e+0
Identity = 419/430 (97.44%), Postives = 426/430 (99.07%), Query Frame = 3
Query:   99 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATV 1388
            MREIVH+QAGQCGNQIGSKFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPG MDSVR+GPFGQLFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT 
Sbjct:    1 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
The following BLAST results are available for this feature:
BLAST of Tubulin beta chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TUBB4B0.000e+096.72tubulin beta 4B class IVb [Source:HGNC Symbol;Acc:... [more]
TUBB2B0.000e+095.35tubulin beta 2B class IIb [Source:HGNC Symbol;Acc:... [more]
TUBB0.000e+095.10tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
TUBB0.000e+095.10tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
TUBB0.000e+095.10tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of Tubulin beta chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tbb-40.000e+094.42Tubulin beta-4 chain [Source:UniProtKB/Swiss-Prot... [more]
ben-10.000e+092.33Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:Q... [more]
mec-70.000e+093.66Tubulin beta-1 chain [Source:UniProtKB/Swiss-Prot... [more]
tbb-20.000e+090.93Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot... [more]
tbb-20.000e+090.93Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot... [more]
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BLAST of Tubulin beta chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
betaTub56D0.000e+095.34gene:FBgn0284243 transcript:FBtr0086536[more]
betaTub85D0.000e+094.42gene:FBgn0003889 transcript:FBtr0082046[more]
betaTub56D0.000e+095.61gene:FBgn0284243 transcript:FBtr0086537[more]
betaTub60D0.000e+089.43gene:FBgn0003888 transcript:FBtr0072270[more]
betaTub60D0.000e+089.63gene:FBgn0003888 transcript:FBtr0343443[more]
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BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tubb4b0.000e+096.50tubulin, beta 4B class IVb [Source:ZFIN;Acc:ZDB-GE... [more]
zgc:554610.000e+096.50zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+096.50zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+096.50zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+096.50zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
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BLAST of Tubulin beta chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
reps20.000e+096.74RALBP1 associated Eps domain containing 2 [Source:... [more]
znf3300.000e+095.35zinc finger protein 330 [Source:Xenbase;Acc:XB-GEN... [more]
tubb0.000e+095.10tubulin beta class I [Source:NCBI gene;Acc:448742][more]
ubxn110.000e+095.32UBX domain protein 11 [Source:Xenbase;Acc:XB-GENE-... [more]
tubb30.000e+091.63tubulin beta 3 class III [Source:NCBI gene;Acc:395... [more]
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BLAST of Tubulin beta chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tubb4b0.000e+096.72tubulin, beta 4B class IVB [Source:MGI Symbol;Acc:... [more]
Tubb2b0.000e+095.35tubulin, beta 2B class IIB [Source:MGI Symbol;Acc:... [more]
Tubb50.000e+095.10tubulin, beta 5 class I [Source:MGI Symbol;Acc:MGI... [more]
Tubb2a0.000e+094.88tubulin, beta 2A class IIA [Source:MGI Symbol;Acc:... [more]
Tubb4a0.000e+095.32tubulin, beta 4A class IVA [Source:MGI Symbol;Acc:... [more]
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BLAST of Tubulin beta chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P30883|TBB4_XENLA0.000e+096.51Tubulin beta-4 chain OS=Xenopus laevis OX=8355 GN=... [more]
sp|P11833|TBB_PARLI0.000e+096.28Tubulin beta chain OS=Paracentrotus lividus OX=765... [more]
sp|P68372|TBB4B_MOUSE0.000e+096.72Tubulin beta-4B chain OS=Mus musculus OX=10090 GN=... [more]
sp|P68371|TBB4B_HUMAN0.000e+096.72Tubulin beta-4B chain OS=Homo sapiens OX=9606 GN=T... [more]
sp|Q3MHM5|TBB4B_BOVIN0.000e+096.72Tubulin beta-4B chain OS=Bos taurus OX=9913 GN=TUB... [more]
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BLAST of Tubulin beta chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2U9BBA20.000e+095.57Ras GTPase-activating-like protein IQGAP1 OS=Scoph... [more]
A0A369S2L50.000e+095.41Tubulin beta chain OS=Trichoplax sp. H2 OX=287889 ... [more]
A0A1I8FZR80.000e+096.51Tubulin beta chain OS=Macrostomum lignano OX=28230... [more]
A0A5S6QE490.000e+096.05Uncharacterized protein OS=Trichuris muris OX=7041... [more]
A0A267E8550.000e+096.51Tubulin beta chain (Fragment) OS=Macrostomum ligna... [more]
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BLAST of Tubulin beta chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:554610.000e+096.50tubulin beta-4B chain [Source:NCBI gene;Acc:103024... [more]
TUBB2A0.000e+093.07tubulin beta-2A chain [Source:NCBI gene;Acc:103041... [more]
TUBB10.000e+079.49tubulin beta 1 class VI [Source:HGNC Symbol;Acc:HG... [more]
ENSAMXT00000019550.20.000e+096.42pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000030346.14.777e-11641.71tubulin alpha-1B chain [Source:NCBI gene;Acc:11119... [more]
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BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tubb20.000e+093.33tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GE... [more]
TUBA3C1.799e-9543.68tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:1240... [more]
ENSPMAT00000009623.13.659e-7984.91pep scaffold:Pmarinus_7.0:GL485233:4234:10583:1 ge... [more]
tubg16.821e-7832.57tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-... [more]
ENSPMAT00000001139.11.858e-7036.65pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 g... [more]
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BLAST of Tubulin beta chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
TUB20.000e+076.74Beta-tubulin; associates with alpha-tubulin (Tub1p... [more]
TUB11.705e-11441.84Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB32.260e-11241.84Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB49.986e-4829.28Gamma-tubulin; involved in nucleating microtubules... [more]
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BLAST of Tubulin beta chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO399450.000e+096.05Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO359580.000e+095.80Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO428900.000e+094.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495180.000e+075.35Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO367340.000e+097.05Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Tubulin beta chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tubb20.000e+094.42tubulin beta-2B chain [Source:NCBI gene;Acc:101162... [more]
tubb50.000e+094.85tubulin beta chain [Source:NCBI gene;Acc:101157969... [more]
ENSORLT00000045809.10.000e+083.45tubulin beta-6 chain [Source:NCBI gene;Acc:1011661... [more]
ENSORLT00000033163.16.169e-11742.17tubulin alpha-1A chain [Source:NCBI gene;Acc:10535... [more]
ENSORLT00000020578.26.169e-11742.17tubulin alpha-1A chain [Source:NCBI gene;Acc:10535... [more]
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BLAST of Tubulin beta chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000044907.10.000e+098.60SMESG000044907.1[more]
SMESG000047291.10.000e+097.91SMESG000047291.1[more]
SMESG000044888.10.000e+097.91SMESG000044888.1[more]
SMESG000047284.10.000e+097.44SMESG000047284.1[more]
SMESG000046374.10.000e+097.44SMESG000046374.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034573 ID=SMED30034573|Name=Tubulin beta chain|organism=Schmidtea mediterranea sexual|type=transcript|length=2309bp
TTTTAAACATATAGTTTCTGTTGTTAAGACTGACAACACTTTTATAACAA
AGAAAATATAAATACTCACGTCCCTCATTAAATTTATTCAAAAACAAAAT
GAGAGAAATCGTTCATTTACAAGCTGGTCAATGCGGTAACCAAATCGGTT
CTAAATTTTGGGAAGTGATTTCTGATGAACATGGTATCGATCCAACCGGA
TCTTATCATGGAGATTCCGATCTTCAGTTAGAACGAATTAATGTTTATTA
TAATGAAGCAACTGGGGGAAAATATGTTCCACGTGCTGTATTAATTGATT
TAGAACCTGGAATAATGGACTCTGTTAGATCGGGGCCGTTTGGTCAACTA
TTCAGACCGGATAATTTTATTTTCGGACAGAGTGGAGCTGGTAATAATTG
GGCCAAAGGTCATTATACCGAAGGAGCTGAATTGGTTGATTCTGTACTCG
ACTGTGTTCGTAAAGAAGCCGAATCTTGTGACTGTTTGCAAGGTTTTCAA
TTGACTCATTCATTAGGAGGAGGAACAGGATCTGGCATGGGAACTCTTCT
TATTTCAAAAATAAGAGAAGAGTATCCAGATAGAATTATGACGACATTTT
CTGTTGTGCCTTCTCCAAAAGTATCCGATACTGTTGTCGAACCATACAAC
GCCACCCTTTCTGTTCATCAACTGGTTGAGAATACAGATGAAACATTCTG
TATCGACAACGAGGCGTTGTACGACATTTGTTTTAGAACATTGAAATTGA
CAACTCCAACCTACGGGGATTTGAATCATTTAGTGAGTGCTACAATGAGT
GGAGTGACAACATGTTTGAGATTCCCTGGTCAATTGAATGCCGATTTGAG
AAAATTAGCAGTTAACATGGTTCCATTCCCTCGTTTGCATTTCTTTATTC
CAGGTTTTGCTCCATTGACCAGTCGAGGAAGTCAACAATATCGTGCATTG
ACCGTTCCAGAATTAACTCAACAAATGTTCGATGCCAAGAACATGATGGC
TGCTTGTGATCCAAGACATGGTCGTTACTTAACAGTAGCTGCTTTGTTCA
GAGGTCGTATGTCAATGAAAGAAGTCGATGAACAAATGTTGAACGTACAA
AACAAGAATAGCAGCTATTTCGTTGAATGGATTCCAAATAACATCAAAAC
CGCAGTTTGTGATATTCCACCAAGAGGATTAAAGATGTCTGCCACATTTA
TTGGTAATAGCACTGCTATTCAAGAGCTATTCAAGCGGGTTAGTGAACAA
TTTACCGCTATGTTTCGTCGAAAGGCTTTCTTGCATTGGTATACTGGTGA
AGGTATGGATGAGATGGAATTCACTGAAGCTGAATCAAACATGAATGATT
TAGTAAGTGAATATCAACAATATCAAGAGGCAACTGTTGAGGATGAAGGC
GATTTTGAAGAGGAAGAAGCAGAGGACCATTAAATTCATATTTATTAATT
TTTTAAGTTGTGGGTTTAAAATAAAAAATATGTTTATTTATAATATTTGT
TTCAAATATTTAATTATTGACTTTTATAATAATAATTGATGTCGAAATTC
GAACTGAAAGCTTCCAGTCTGCCAAACACTAGGAAGCTAAGCAAACAACT
AAAACCGATGCCTGATTTGAAATTCAAGCTGTCTGGACACATTAATCCGT
ACCTTCCGCCAATTCTAGAGCCTAAAATGCCCAATGACATTCTGGAAACA
ATAGAAAAGCACATAGAAATTTTCAAAAATGAAAATTCGTTTTATTTTCT
TTCAATTTCCGAATCAAAACTCTGTCACATTGCAAAAGATTATAATTCTT
TTTTCAAGAAATTTTTAAGGAATATTGTAATAGATGTAGTTGTTACAAAG
CGATTTGATTTTTCTGAACATGACAGTCGAGGGTATTCAATCCTTTTAAT
TACCCATGTCATAGGATTAGCAATAAAGTATTTTCAAGCGTTATTTTTCC
CAAAATCGAAAGTTGTTGTTTATGCAGGGATAGTCGAGAGGAAAGACTTA
ACCAGCTTTACTAACAGCAGCCGCTGCTTATGGGTCCCCAAAGATGGTGA
CACATTTATCAATTTGAAATTAAAATTCGAAGCTGCATATTTGGTGGCCA
CCACTTATATCATGAGTAGTTATAAAAATTGTTCCAGGGAATAGTGCAAA
AAATGTATTTAATAAATATTTTTTAAATTCAGTTATAATTTCAAATTTAG
TTGGTCATTGTTGGTACTTAGACTTTCTTGTACATTCAGATTAATAGAGC
TTTTGTTTATTAAACAATTTGTGCTATTCAATTGGATACGCTTGAGTTAA
AATGAAATA
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protein sequence of SMED30034573-orf-1

>SMED30034573-orf-1 ID=SMED30034573-orf-1|Name=SMED30034573-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=445bp
MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLERINVY
YNEATGGKYVPRAVLIDLEPGIMDSVRSGPFGQLFRPDNFIFGQSGAGNN
WAKGHYTEGAELVDSVLDCVRKEAESCDCLQGFQLTHSLGGGTGSGMGTL
LISKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETF
CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADL
RKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMM
AACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIK
TAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTG
EGMDEMEFTEAESNMNDLVSEYQQYQEATVEDEGDFEEEEAEDH*
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protein sequence of SMED30034573-orf-2

>SMED30034573-orf-2 ID=SMED30034573-orf-2|Name=SMED30034573-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=202bp
MSKFELKASSLPNTRKLSKQLKPMPDLKFKLSGHINPYLPPILEPKMPND
ILETIEKHIEIFKNENSFYFLSISESKLCHIAKDYNSFFKKFLRNIVIDV
VVTKRFDFSEHDSRGYSILLITHVIGLAIKYFQALFFPKSKVVVYAGIVE
RKDLTSFTNSSRCLWVPKDGDTFINLKLKFEAAYLVATTYIMSSYKNCSR
E*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0000103central nervous system
PLANA:0000418head
PLANA:0000429neoblast
Vocabulary: INTERPRO
TermDefinition
IPR036525Tubulin/FtsZ_GTPase_sf
IPR037103Tubulin/FtsZ_C_sf
IPR000217Tubulin
IPR002453Beta_tubulin
IPR018316Tubulin/FtsZ_2-layer-sand-dom
IPR003008Tubulin_FtsZ_GTPase
IPR023123Tubulin_C
IPR017975Tubulin_CS
IPR008280Tub_FtsZ_C
Vocabulary: molecular function
TermDefinition
GO:0003924GTPase activity
GO:0005525GTP binding
GO:0005200structural constituent of cytoskeleton
GO:0000166nucleotide binding
Vocabulary: biological process
TermDefinition
GO:0007017microtubule-based process
GO:0007010cytoskeleton organization
Vocabulary: cellular component
TermDefinition
GO:0005874microtubule
GO:0005737cytoplasm
GO:0005856cytoskeleton
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 403..423
NoneNo IPR availablePANTHERPTHR11588:SF340TUBULIN BETA CHAINcoord: 1..435
NoneNo IPR availableCDDcd02187beta_tubulincoord: 1..421
e-value: 0.0
score: 888.067
IPR000217TubulinPRINTSPR01161TUBULINcoord: 88..99
score: 76.26
coord: 171..184
score: 79.11
coord: 185..205
score: 69.69
coord: 365..393
score: 67.67
coord: 101..125
score: 67.92
coord: 146..167
score: 57.47
coord: 5..25
score: 73.73
coord: 127..145
score: 79.22
coord: 46..65
score: 68.78
IPR000217TubulinPANTHERPTHR11588TUBULINcoord: 1..435
IPR002453Beta tubulinPRINTSPR01163BETATUBULINcoord: 225..234
score: 83.43
coord: 207..219
score: 85.45
coord: 306..324
score: 76.48
coord: 260..276
score: 80.57
coord: 324..338
score: 89.56
coord: 372..383
score: 83.43
coord: 83..94
score: 88.81
coord: 407..425
score: 87.67
coord: 240..253
score: 89.06
coord: 147..159
score: 90.86
coord: 342..365
score: 78.19
coord: 36..53
score: 81.78
coord: 103..121
score: 83.19
IPR018316Tubulin/FtsZ, 2-layer sandwich domainSMARTSM00865Tubulin_C_4coord: 241..378
e-value: 2.9E-48
score: 176.3
IPR018316Tubulin/FtsZ, 2-layer sandwich domainPFAMPF03953Tubulin_Ccoord: 256..377
e-value: 4.4E-42
score: 143.2
IPR003008Tubulin/FtsZ, GTPase domainSMARTSM00864Tubulin_4coord: 42..239
e-value: 1.8E-69
score: 246.8
IPR003008Tubulin/FtsZ, GTPase domainPFAMPF00091Tubulincoord: 2..206
e-value: 7.4E-68
score: 228.6
IPR036525Tubulin/FtsZ, GTPase domain superfamilyGENE3DG3DSA:3.40.50.1440coord: 1..258
e-value: 2.1E-125
score: 419.6
IPR036525Tubulin/FtsZ, GTPase domain superfamilySUPERFAMILYSSF52490Tubulin nucleotide-binding domain-likecoord: 1..238
IPR023123Tubulin, C-terminalGENE3DG3DSA:1.10.287.600coord: 369..439
e-value: 5.5E-35
score: 121.1
IPR037103Tubulin/FtsZ, C-terminal domain superfamilyGENE3DG3DSA:3.30.1330.20coord: 259..368
e-value: 8.6E-57
score: 192.2
IPR017975Tubulin, conserved sitePROSITEPS00227TUBULINcoord: 135..141
IPR008280Tubulin/FtsZ, C-terminalSUPERFAMILYSSF55307Tubulin C-terminal domain-likecoord: 239..424