Tetraspanin

Overview
NameTetraspanin
Smed IDSMED30034552
Length (bp)1266
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Tetraspanin (SMED30034552) t-SNE clustered cells

Violin plots show distribution of expression levels for Tetraspanin (SMED30034552) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Tetraspanin (SMED30034552) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Tetraspanin (SMED30034552) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30034552

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30034552h1SMcG0004272 SmedASXL_009981SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30034552h1SMcG0004272 SmedASXL_009981SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30034552h1SMcG0004272 dd_Smed_v6_4902_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30034552h1SMcG0004272 OX_Smed_1.0.08430ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Tetraspanin vs. Ensembl Human
Match: TSPAN3 (tetraspanin 3 [Source:HGNC Symbol;Acc:HGNC:17752])

HSP 1 Score: 55.4546 bits (132), Expect = 2.366e-8
Identity = 33/110 (30.00%), Postives = 58/110 (52.73%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKY 1043
            K+ N V++++ K+ + Y G N  +  S  I+ +Q Q HCCG++NYSD++  + W         PLSCC  RE      + C  +   PS    +GC + +  +L++ + +
Sbjct:   46 KVENEVDRSIQKVYKTYNGTNP-DAASRAIDYVQRQLHCCGIHNYSDWENTD-WFKETKNQSVPLSCC--RE----TASNCNGSLAHPSDLYAEGCEALVVKKLQEIMMH 147
[Close]
BLAST of Tetraspanin vs. Ensembl Human
Match: TSPAN3 (tetraspanin 3 [Source:HGNC Symbol;Acc:HGNC:17752])

HSP 1 Score: 54.6842 bits (130), Expect = 2.564e-8
Identity = 35/111 (31.53%), Postives = 58/111 (52.25%), Query Frame = 3 
Query:  732 YVEKNLYKLIEK-YEGYNATNVE--SLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKYAF 1049
            +VE  + + I+K Y+ YN TN +  S  I+ +Q Q HCCG++NYSD++  + W         PLSCC  RE      + C  +   PS    +GC + +  +L++ + +  
Sbjct:   21 WVENEVDRSIQKVYKTYNGTNPDAASRAIDYVQRQLHCCGIHNYSDWENTD-WFKETKNQSVPLSCC--RE----TASNCNGSLAHPSDLYAEGCEALVVKKLQEIMMHVI 124
[Close]
BLAST of Tetraspanin vs. Ensembl Human
Match: TSPAN3 (tetraspanin 3 [Source:HGNC Symbol;Acc:HGNC:17752])

HSP 1 Score: 55.0694 bits (131), Expect = 5.877e-8
Identity = 33/110 (30.00%), Postives = 58/110 (52.73%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKY 1043
            K+ N V++++ K+ + Y G N  +  S  I+ +Q Q HCCG++NYSD++  + W         PLSCC  RE      + C  +   PS    +GC + +  +L++ + +
Sbjct:  110 KVENEVDRSIQKVYKTYNGTNP-DAASRAIDYVQRQLHCCGIHNYSDWENTD-WFKETKNQSVPLSCC--RE----TASNCNGSLAHPSDLYAEGCEALVVKKLQEIMMH 211
[Close]
BLAST of Tetraspanin vs. Ensembl Human
Match: TSPAN3 (tetraspanin 3 [Source:HGNC Symbol;Acc:HGNC:17752])

HSP 1 Score: 54.299 bits (129), Expect = 9.168e-8
Identity = 33/113 (29.20%), Postives = 58/113 (51.33%), Query Frame = 3 
Query:  711 GNQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKY 1043
            G   + N V++++ K+ + Y G N  +  S  I+ +Q Q HCCG++NYSD++  + W         PLSCC  RE      + C  +   PS    +GC + +  +L++ + +
Sbjct:   82 GLATVENEVDRSIQKVYKTYNGTNP-DAASRAIDYVQRQLHCCGIHNYSDWENTD-WFKETKNQSVPLSCC--RE----TASNCNGSLAHPSDLYAEGCEALVVKKLQEIMMH 186
[Close]
BLAST of Tetraspanin vs. Ensembl Zebrafish
Match: tspan3a (tetraspanin 3a [Source:ZFIN;Acc:ZDB-GENE-030131-7787])

HSP 1 Score: 55.0694 bits (131), Expect = 3.378e-8
Identity = 39/134 (29.10%), Postives = 67/134 (50.00%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKK---YLKYAFLTLGLSITILAVLDTMFIL 1106
            K+ + V  ++ K+  +Y G N T+  S  I+ +Q Q HCCG+ N++D+ R  +W         PLSCC+       N++ C  +   P     +GC   +  +LK+   Y+ +A LT   +I +L +L    +L
Sbjct:  110 KVEDEVNHSIQKVYNEYNGTN-TDAPSRAIDYVQRQLHCCGITNFADW-RNTRWFKESKNNSVPLSCCKP------NVSNCTGSLLHPGDLYPEGCEELVVKKLKEIMMYVIWAALTFA-AIQMLGMLCACVVL 234
[Close]
BLAST of Tetraspanin vs. Ensembl Zebrafish
Match: tspan3a (tetraspanin 3a [Source:ZFIN;Acc:ZDB-GENE-030131-7787])

HSP 1 Score: 55.0694 bits (131), Expect = 3.378e-8
Identity = 39/134 (29.10%), Postives = 67/134 (50.00%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKK---YLKYAFLTLGLSITILAVLDTMFIL 1106
            K+ + V  ++ K+  +Y G N T+  S  I+ +Q Q HCCG+ N++D+ R  +W         PLSCC+       N++ C  +   P     +GC   +  +LK+   Y+ +A LT   +I +L +L    +L
Sbjct:  110 KVEDEVNHSIQKVYNEYNGTN-TDAPSRAIDYVQRQLHCCGITNFADW-RNTRWFKESKNNSVPLSCCKP------NVSNCTGSLLHPGDLYPEGCEELVVKKLKEIMMYVIWAALTFA-AIQMLGMLCACVVL 234
[Close]
BLAST of Tetraspanin vs. Ensembl Zebrafish
Match: tspan3a (tetraspanin 3a [Source:ZFIN;Acc:ZDB-GENE-030131-7787])

HSP 1 Score: 55.0694 bits (131), Expect = 3.378e-8
Identity = 39/134 (29.10%), Postives = 67/134 (50.00%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKK---YLKYAFLTLGLSITILAVLDTMFIL 1106
            K+ + V  ++ K+  +Y G N T+  S  I+ +Q Q HCCG+ N++D+ R  +W         PLSCC+       N++ C  +   P     +GC   +  +LK+   Y+ +A LT   +I +L +L    +L
Sbjct:  110 KVEDEVNHSIQKVYNEYNGTN-TDAPSRAIDYVQRQLHCCGITNFADW-RNTRWFKESKNNSVPLSCCKP------NVSNCTGSLLHPGDLYPEGCEELVVKKLKEIMMYVIWAALTFA-AIQMLGMLCACVVL 234
[Close]
BLAST of Tetraspanin vs. Ensembl Zebrafish
Match: tspan3b (tetraspanin 3b [Source:ZFIN;Acc:ZDB-GENE-070424-42])

HSP 1 Score: 51.9878 bits (123), Expect = 4.352e-7
Identity = 33/113 (29.20%), Postives = 53/113 (46.90%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKYAFL 1052
            K+   V  ++ K+  +Y+G N T+  S  I+ +Q Q HCCG++NYSD+     W         P+SCC+        ++ C  T   P     +GC   +  +LK  + Y  L
Sbjct:  110 KVEAVVNHSIQKVYNEYKGTN-TDAPSRAIDYVQRQLHCCGIHNYSDWMNTH-WFIESKNNSVPVSCCKP------TISNCTGTLMRPGDLYPEGCEVLVVKKLKDIMLYVIL 214
[Close]
BLAST of Tetraspanin vs. Ensembl Zebrafish
Match: tspan3b (tetraspanin 3b [Source:ZFIN;Acc:ZDB-GENE-070424-42])

HSP 1 Score: 51.9878 bits (123), Expect = 4.352e-7
Identity = 33/113 (29.20%), Postives = 53/113 (46.90%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKYAFL 1052
            K+   V  ++ K+  +Y+G N T+  S  I+ +Q Q HCCG++NYSD+     W         P+SCC+        ++ C  T   P     +GC   +  +LK  + Y  L
Sbjct:  110 KVEAVVNHSIQKVYNEYKGTN-TDAPSRAIDYVQRQLHCCGIHNYSDWMNTH-WFIESKNNSVPVSCCKP------TISNCTGTLMRPGDLYPEGCEVLVVKKLKDIMLYVIL 214
[Close]
BLAST of Tetraspanin vs. Ensembl Xenopus
Match: lrrk2 (leucine-rich repeat kinase 2 [Source:Xenbase;Acc:XB-GENE-5768572])

HSP 1 Score: 56.225 bits (134), Expect = 2.041e-8
Identity = 52/182 (28.57%), Postives = 92/182 (50.55%), Query Frame = 3 
Query:  546 GAIAFSIGVLFIAIAILGYLSLC---CCGAKCFXXXXXXXXXXXXXXXXXXXXXYGAQGNQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCE--SREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKYLKYA-FLTLGLSIT 1073
              I  +I V    IAILG  S     CCG  CF+++I  I  A + ++++     G     KI   +EKN+  L   ++ YN  +V S  ++ IQ +  CCG  NY+D++  + +   K     P+SCC+  +++ + V + + KD +     T+ C   ++  + + L+Y+ F+ LG +I 
Sbjct:   53 SVIILAIAVFMFVIAILGCCSTTQESCCGLGCFMFLISIIFAAGVAAMIL-----GLVFVDKINPELEKNMDNL---FKTYNGVDVSSSTVDFIQERLECCGRKNYTDWEETDWYQTNK---SLPMSCCKKNAQDCQRV-IGQQKDIY-----TEACEPKLENLIHQVLRYSMFVILGFAIV 217
[Close]
BLAST of Tetraspanin vs. Ensembl Xenopus
Match: lrrk2 (leucine-rich repeat kinase 2 [Source:Xenbase;Acc:XB-GENE-5768572])

HSP 1 Score: 55.8398 bits (133), Expect = 2.687e-8
Identity = 53/184 (28.80%), Postives = 93/184 (50.54%), Query Frame = 3 
Query:  546 GAIAFSIGVLFIAIAILGYLSLC---CCGAKCFXXXXXXXXXXXXXXXXXXXXXYGAQGNQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCE--SREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKYLKYA-FLTLGLSITIL 1079
              I  +I V    IAILG  S     CCG  CF+++I  I  A + ++++     G     KI   +EKN+  L   ++ YN  +V S  ++ IQ +  CCG  NY+D++  + +   K     P+SCC+  +++ + V + + KD +     T+ C   ++  + + L+Y+ F+ LG +I  L
Sbjct:   60 SVIILAIAVFMFVIAILGCCSTTQESCCGLGCFMFLISIIFAAGVAAMIL-----GLVFVDKINPELEKNMDNL---FKTYNGVDVSSSTVDFIQERLECCGRKNYTDWEETDWYQTNK---SLPMSCCKKNAQDCQRV-IGQQKDIY-----TEACEPKLENLIHQVLRYSMFVILGFAIVEL 226
[Close]
BLAST of Tetraspanin vs. Ensembl Xenopus
Match: fam110d (family with sequence similarity 110 member D [Source:Xenbase;Acc:XB-GENE-920630])

HSP 1 Score: 48.9062 bits (115), Expect = 5.111e-6
Identity = 31/112 (27.68%), Postives = 58/112 (51.79%), Query Frame = 3 
Query:  726 FNYVEK---NLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCC-ESREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKYLKYAF 1049
            F Y+++    L  ++E +E Y+ +  + + +N IQ +  CCGL+NY+D++ A  W   +  F  P +CC E+    Y N  E K  +      +GC+  +  +L  ++ + F
Sbjct:  105 FLYMDRMDFELKPMLEAFENYDGSKSD-VTVNKIQKELRCCGLHNYTDWE-ATSWY--RQNFTIPKTCCNETYTTCYGNTTESKAFY-----QEGCFDKLHHRLNYFMTWLF 207
[Close]
BLAST of Tetraspanin vs. Ensembl Xenopus
Match: gpr88 (G protein-coupled receptor 88 [Source:Xenbase;Acc:XB-GENE-984668])

HSP 1 Score: 48.9062 bits (115), Expect = 5.584e-6
Identity = 33/112 (29.46%), Postives = 53/112 (47.32%), Query Frame = 3 
Query:  762 EKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCC----ESREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKYLKYAFLTLGLSITILAV 1085
            + Y G N+T V S   N I     CCG+    DF+ A ++         P +CC    +SR  + V+ AEC     +    +GCYS I   ++ Y+   ++   L+I +LA+
Sbjct:  123 KHYHGDNSTEVFSSTWNSIMITFGCCGVNGPEDFNDAHRFRAMHPFAPVPEACCRREVQSRAGKIVSRAECLTGGENYQNRQGCYSVIVNSVEPYV---YIAGALAIGVLAI 231
[Close]
BLAST of Tetraspanin vs. Ensembl Mouse
Match: Tspan3 (tetraspanin 3 [Source:MGI Symbol;Acc:MGI:1928098])

HSP 1 Score: 55.8398 bits (133), Expect = 2.077e-8
Identity = 34/110 (30.91%), Postives = 59/110 (53.64%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKY 1043
            K+ N V++++ K+ + Y G N ++  S  I+ +Q Q HCCG++NYSD++  + W         PLSCC  RE        C  +  +PS    +GC + +  +L++ L +
Sbjct:  110 KVENEVDRSIQKVYKTYNGTN-SDAASRAIDYVQRQLHCCGIHNYSDWENTD-WFKETKNQSVPLSCC--RE----TAKSCNGSLANPSDLYAEGCEALVVKKLQEILMH 211
[Close]
BLAST of Tetraspanin vs. Ensembl Mouse
Match: Tspan3 (tetraspanin 3 [Source:MGI Symbol;Acc:MGI:1928098])

HSP 1 Score: 55.8398 bits (133), Expect = 2.116e-8
Identity = 34/110 (30.91%), Postives = 59/110 (53.64%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKY 1043
            K+ N V++++ K+ + Y G N ++  S  I+ +Q Q HCCG++NYSD++  + W         PLSCC  RE        C  +  +PS    +GC + +  +L++ L +
Sbjct:  113 KVENEVDRSIQKVYKTYNGTN-SDAASRAIDYVQRQLHCCGIHNYSDWENTD-WFKETKNQSVPLSCC--RE----TAKSCNGSLANPSDLYAEGCEALVVKKLQEILMH 214
[Close]
BLAST of Tetraspanin vs. UniProt/SwissProt
Match: sp|Q9QY33|TSN3_MOUSE (Tetraspanin-3 OS=Mus musculus OX=10090 GN=Tspan3 PE=1 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.453e-7
Identity = 34/110 (30.91%), Postives = 59/110 (53.64%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKY 1043
            K+ N V++++ K+ + Y G N ++  S  I+ +Q Q HCCG++NYSD++  + W         PLSCC  RE        C  +  +PS    +GC + +  +L++ L +
Sbjct:  110 KVENEVDRSIQKVYKTYNGTN-SDAASRAIDYVQRQLHCCGIHNYSDWENTD-WFKETKNQSVPLSCC--RE----TAKSCNGSLANPSDLYAEGCEALVVKKLQEILMH 211
[Close]
BLAST of Tetraspanin vs. UniProt/SwissProt
Match: sp|Q3SZR9|TSN3_BOVIN (Tetraspanin-3 OS=Bos taurus OX=9913 GN=TSPAN3 PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.916e-7
Identity = 33/110 (30.00%), Postives = 59/110 (53.64%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKY 1043
            K+ N V++++ K+ + Y G N  +  S  I+ +Q Q HCCG++NYSD++  + W         PLSCC  RE      + C  +  +PS    +GC + +  +L++ + +
Sbjct:  110 KVENEVDRSIQKVYKTYNGTNP-DAASRAIDYVQRQLHCCGIHNYSDWENTD-WFKETKNQSVPLSCC--RE----TASSCNGSLANPSDLYAEGCEALVVKKLQEIMMH 211
[Close]
BLAST of Tetraspanin vs. UniProt/SwissProt
Match: sp|Q5RE11|TSN3_PONAB (Tetraspanin-3 OS=Pongo abelii OX=9601 GN=TSPAN3 PE=2 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 2.822e-7
Identity = 33/110 (30.00%), Postives = 58/110 (52.73%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKY 1043
            K+ N V++++ K+ + Y G N  +  S  I+ +Q Q HCCG++NYSD++  + W         PLSCC  RE      + C  +   PS    +GC + +  +L++ + +
Sbjct:  110 KVENEVDRSIQKVYKTYNGTNP-DAASRAIDYVQRQLHCCGIHNYSDWENTD-WFKETKNQSVPLSCC--RE----TASNCNGSLAHPSDLYAEGCEALVVKKLQEIMMH 211
[Close]
BLAST of Tetraspanin vs. UniProt/SwissProt
Match: sp|O60637|TSN3_HUMAN (Tetraspanin-3 OS=Homo sapiens OX=9606 GN=TSPAN3 PE=2 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 2.822e-7
Identity = 33/110 (30.00%), Postives = 58/110 (52.73%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKY 1043
            K+ N V++++ K+ + Y G N  +  S  I+ +Q Q HCCG++NYSD++  + W         PLSCC  RE      + C  +   PS    +GC + +  +L++ + +
Sbjct:  110 KVENEVDRSIQKVYKTYNGTNP-DAASRAIDYVQRQLHCCGIHNYSDWENTD-WFKETKNQSVPLSCC--RE----TASNCNGSLAHPSDLYAEGCEALVVKKLQEIMMH 211
[Close]
BLAST of Tetraspanin vs. TrEMBL
Match: A0A419PZD4 (Tetraspanin-6 (Fragment) OS=Clonorchis sinensis OX=79923 GN=Tspan6 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.035e-10
Identity = 50/208 (24.04%), Postives = 98/208 (47.12%), Query Frame = 3 
Query:  444 DLSGHMKKLLQNIHVNANGIKSFEEVISA--NSATIGAIAFSIGVLFIAIAILGYLSLCCCGAKCFXXXXXXXXXXXXXXXXXXXXXYGAQGNQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRK-------LGFKFPLSCCESREMRYVNLAECKDTWTS--PSLTKGCYSSIKPQLKKY 1034
             + G +K+ +  ++ +   I++ E    A   +A  G I FS G +   + + G +  CC  A C    +  +I   ++ ++ +A  Y  +    +FN V + L + + +Y    + +  S++ NV+    HCCGL+N SDFDRA+ +  R+       +   +P++CC+      +    C   + S   ++ +GC+  + P L  Y
Sbjct:   41 QIRGFLKQAIVTLYGHKTAIEATELAQHALRITAPFGMIIFSFGTIIFLLCVFGCIGACCNSALCLKIYLGFLIFFTVLEIVALAFYYSRRST--VFNIVRRILKQSLLRYRSKESRDPHSMLFNVMMPPLHCCGLHNASDFDRAKDF-DRQFNTTTGLINITYPVTCCKMDSAYVIQDNTCPGRFDSNNSNIDRGCWEQLYPHLVYY 245
[Close]
BLAST of Tetraspanin vs. TrEMBL
Match: A0A099ZMK9 (Tetraspanin (Fragment) OS=Tinamus guttatus OX=94827 GN=N309_14827 PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 3.082e-9
Identity = 42/122 (34.43%), Postives = 61/122 (50.00%), Query Frame = 3 
Query:  714 NQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPSL--TKGCYSSIKPQLKKYLKYAFLT-LGLSI 1070
             +K+   V+  +  + EKY+G NA   ES ++N +Q Q HCCG+ NYSD+  + +W         PLSCC         L+ C  +   P    TKGC   ++  L+  L YA L  LG +I
Sbjct:   91 REKVKTDVQDTMRSVFEKYDGKNA---ESTVLNYLQEQLHCCGVRNYSDW-TSTQWFNSTGKNSVPLSCCRQ------GLSNCTGSLDQPQELNTKGCAGELESGLQSVLSYAMLVILGFAI 202
[Close]
BLAST of Tetraspanin vs. TrEMBL
Match: A0A075AI31 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_02737 PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 5.047e-9
Identity = 59/223 (26.46%), Postives = 108/223 (48.43%), Query Frame = 3 
Query:  447 LSGHMKKLLQNIHVNANGIKSFEEV--ISANSATIGAIAFSIGVLFIAIAILGYLSLCCCGAKCFXXXXXXXXXXXXXXXXXXXXXYGAQGNQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDF---DRAEKWVGRK-LGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKY-AFLTLGLSITILAVL 1088
            ++G +  LLQ+I V  +  +  E +  I   ++ IG   F +G +F  ++I+GY    CC  K  +Y+  A++  L ++++    +Y A    KI +YV +   K ++ Y    A  V+SL + +IQ    CCG+ + +DF      + + G+   G   P+ CC   +   +    C  T+TS +  +  GC   +K     Y+ Y A+  +G  + +L V+
Sbjct:   43 INGFLNPLLQSISVTQDAAQVTELIGRILTATSPIGITLFVLGAIFAIVSIIGYCG-ACCNYKVLLYLYAALVGVLALAVMACFSIYFAN-KDKIADYVVEVFAKSVKAYTSMEANTVDSLTVGLIQPPLKCCGVTSSADFVNLSSEDTYGGQSYTGLTAPIPCCMMNDKYQITGVGCPATFTSANSYVHVGCKDPLKSNFIYYMNYLAYGLIGAFVILLLVV 263
[Close]
BLAST of Tetraspanin vs. TrEMBL
Match: R7VAV1 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_216192 PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 7.114e-9
Identity = 57/244 (23.36%), Postives = 122/244 (50.00%), Query Frame = 3 
Query:  444 DLSGHMKKLLQNIHVNANGIKSFEEVISANSATIGAIAFSIGVLFIAIAILGYLSLC--CCGAKCFXXXXXXXXXXXXXXXXXXXXXYGAQGNQKIFNYVEKN-LYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRK----------LGFKFPLSCCESREMRYVN-LAECKDTWTSPS-----LTKGCYSSIKPQLKKYLKYAFLTLG--LSITILAVLDTMFILFL 1112
            +L    +++L  I  + +  ++ +E++        ++  +I ++ + I +L  L+ C  CC  KC +   I ++  +++   +   +YG     K+   ++K+ L  L EKYEG    N+ES+++N++Q Q  CCG+ N +DFD  + W   +          +  + P +CC+   +   + + + ++  T+P+     +  GCY ++K ++K +  Y    +G  +++ +L VL    IL+L
Sbjct:   47 NLKDTFEEVLNAITGDKSNAEAAQELVDQFFGLFMSLGVAILMIGLLIFVLTILASCGSCCMVKCMLIGYIVLLVVVILVQAICLGLYGTD---KLDAPIKKSSLKSLKEKYEGNKGDNLESIMLNLVQYQFECCGINNVTDFDDVDNWDKSEGYEHNGQTYVVEVELPTTCCKMTGIFPDDVILQDQNCPTAPTSVNSYMATGCYEAVKAEMKTFQVYILGFVGGCIALELLFVLLAALILYL 287
[Close]
BLAST of Tetraspanin vs. TrEMBL
Match: A0A074ZGR1 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_09476 PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 9.085e-9
Identity = 47/207 (22.71%), Postives = 96/207 (46.38%), Query Frame = 3 
Query:  444 DLSGHMKKLLQNIHVNANGIKSFEEVISA--NSATIGAIAFSIGVLFIAIAILGYLSLCCCGAKCFXXXXXXXXXXXXXXXXXXXXXYGAQGNQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGR------KLGFKFPLSCCESREMRYVNLAECKDTWTS--PSLTKGCYSSIKPQLKKY 1034
             + G +K+ +  ++ +   I++ E    A   +A  G I FS G +   + + G +   C  A C    +  +I   ++ ++ +A  Y  +    +FN V + L + + +Y    + +  S++ NV+    HCCGL+N SDFD+A+ +  +       +   +P++CC+      +    C   + S   ++ +GC+  + P L  Y
Sbjct:   41 QIRGFLKQAIVMLYGHKTAIEATELAQHALRITAPFGMIIFSFGTIIFLLCVFGCIGASCNSALCLKIYLGFLIFFTVLEIVALAFYYSRRST--VFNIVRRILKQSLLRYRSKESRDAHSMLFNVMMPPLHCCGLHNASDFDQAKDFDHQYNTTTGLINITYPVTCCKMDHSYVIQDNTCPGKFDSNNSNIDRGCWEQLYPHLVYY 245
[Close]
BLAST of Tetraspanin vs. Ensembl Cavefish
Match: tspan36 (tetraspanin 36 [Source:NCBI gene;Acc:437021])

HSP 1 Score: 55.8398 bits (133), Expect = 1.648e-8
Identity = 37/123 (30.08%), Postives = 64/123 (52.03%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPSL--TKGCYSSIKPQLKKYLKYAFLTLG-LSITIL 1079
            KI   +EK++  + +KY+G NA   E+  ++ +Q+Q  CCG+ NY+D+  +  W         P+SCC++        + C   +  P L   +GC + ++  L+  L YA L  G LSI ++
Sbjct:  109 KIKGDLEKSMSDVFQKYDGKNA---ETAAVDYLQSQLQCCGVNNYTDWS-SRPWFTSH-NSTVPVSCCKAN-------STCTGQFDKPELLNVQGCEAKLEKLLQDVLSYAMLFFGMLSICVI 219
[Close]
BLAST of Tetraspanin vs. Ensembl Cavefish
Match: tspan3a (tetraspanin 3 [Source:NCBI gene;Acc:103046031])

HSP 1 Score: 55.4546 bits (132), Expect = 1.952e-8
Identity = 42/132 (31.82%), Postives = 66/132 (50.00%), Query Frame = 3 
Query:  735 VEKNLYKLIEK-YEGYNATNVE--SLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKK---YLKYAFLTLGLSITILAVLDTMFIL 1106
            VE+ +   I+K Y  YN TN +  S  I+ +Q Q HCCG+ NY D+ R   W         PLSCC+       N++ C  + + P     +GC + +  +LK+   Y+ +A LT   +I +L +L    +L
Sbjct:   87 VEEEVNHSIQKVYNEYNGTNSDAPSRAIDYVQRQLHCCGITNYLDW-RNTSWFNESRNNSVPLSCCKP------NVSNCTGSLSRPGDLYPEGCEALVVKKLKEIMMYVIWAALTFA-AIQMLGMLCACVVL 210
[Close]
BLAST of Tetraspanin vs. Ensembl Cavefish
Match: tspan3a (tetraspanin 3 [Source:NCBI gene;Acc:103046031])

HSP 1 Score: 55.4546 bits (132), Expect = 2.809e-8
Identity = 42/132 (31.82%), Postives = 66/132 (50.00%), Query Frame = 3 
Query:  735 VEKNLYKLIEK-YEGYNATNVE--SLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKK---YLKYAFLTLGLSITILAVLDTMFIL 1106
            VE+ +   I+K Y  YN TN +  S  I+ +Q Q HCCG+ NY D+ R   W         PLSCC+       N++ C  + + P     +GC + +  +LK+   Y+ +A LT   +I +L +L    +L
Sbjct:  113 VEEEVNHSIQKVYNEYNGTNSDAPSRAIDYVQRQLHCCGITNYLDW-RNTSWFNESRNNSVPLSCCKP------NVSNCTGSLSRPGDLYPEGCEALVVKKLKEIMMYVIWAALTFA-AIQMLGMLCACVVL 236
[Close]
BLAST of Tetraspanin vs. Ensembl Cavefish
Match: tspan3a (tetraspanin 3 [Source:NCBI gene;Acc:103046031])

HSP 1 Score: 55.0694 bits (131), Expect = 3.168e-8
Identity = 42/132 (31.82%), Postives = 66/132 (50.00%), Query Frame = 3 
Query:  735 VEKNLYKLIEK-YEGYNATNVE--SLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKK---YLKYAFLTLGLSITILAVLDTMFIL 1106
            VE+ +   I+K Y  YN TN +  S  I+ +Q Q HCCG+ NY D+ R   W         PLSCC+       N++ C  + + P     +GC + +  +LK+   Y+ +A LT   +I +L +L    +L
Sbjct:  112 VEEEVNHSIQKVYNEYNGTNSDAPSRAIDYVQRQLHCCGITNYLDW-RNTSWFNESRNNSVPLSCCKP------NVSNCTGSLSRPGDLYPEGCEALVVKKLKEIMMYVIWAALTFA-AIQMLGMLCACVVL 235
[Close]
BLAST of Tetraspanin vs. Ensembl Cavefish
Match: tspan36 (tetraspanin 36 [Source:NCBI gene;Acc:437021])

HSP 1 Score: 53.9138 bits (128), Expect = 7.025e-8
Identity = 33/115 (28.70%), Postives = 59/115 (51.30%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPSL--TKGCYSSIKPQLKKYLKYAFLTL 1058
            KI   +EK++  + +KY+G NA   E+  ++ +Q+Q  CCG+ NY+D+  +  W         P+SCC++        + C   +  P L   +GC + ++  L+  L YA L +
Sbjct:  109 KIKGDLEKSMSDVFQKYDGKNA---ETAAVDYLQSQLQCCGVNNYTDWS-SRPWFTSH-NSTVPVSCCKAN-------STCTGQFDKPELLNVQGCEAKLEKLLQDVLSYAMLVI 211
[Close]
BLAST of Tetraspanin vs. Ensembl Sea Lamprey
Match: tspan4a (tetraspanin 4a [Source:ZFIN;Acc:ZDB-GENE-040718-38])

HSP 1 Score: 50.0618 bits (118), Expect = 2.664e-7
Identity = 41/138 (29.71%), Postives = 67/138 (48.55%), Query Frame = 3 
Query:  723 IFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSD-FDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTS-PSLTKGCYSSIKPQLKKYLKYAFLTLGLSITILAVLDTMFILFLIFENRS 1130
            I +Y +K+L   ++ Y       + +   N++QT  HCCG+ NY+D FD  +K        + P SCC    + Y N   C    TS  ++  GCY  +K  L+++L    +  GL I ++ VL   F + +  + RS
Sbjct:  112 IDSYAQKDLIHGLKLYGQQGNVGLTN-AWNIVQTDFHCCGVGNYTDWFDVFKK-------KQVPDSCC----LEYSN--GCGSILTSITNINSGCYDKVKAWLQEHLMVVGI-FGLCIALIQVLGMAFAMIMYCQLRS 234
[Close]
BLAST of Tetraspanin vs. Ensembl Nematostella
Match: EDO39847 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S901])

HSP 1 Score: 65.4698 bits (158), Expect = 3.545e-12
Identity = 67/211 (31.75%), Postives = 104/211 (49.29%), Query Frame = 3 
Query:  507 SFEEVISANSATIGAIAFSIGVLFIAIAILGYLSLCCCGAKCFXXXXXXXXXXXXXXXXXXXXXYGA---QGNQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKYLKYAFLTLGLSITILAVLDTMFILFLIFENRS 1130
            ++ E+ S N AT   +  ++G +   +A LG     CCGA      ++    A L+ LL++ IV GA       KI N ++K++ K IE Y G N  +     IN +QT+  CCG  NY+D+    K    K     P SCC+S E     +   KD       TKGC++ IK +++K LK     L +++ ++ +L  +F L LI   +S
Sbjct:   41 AYVEIASVNYATGPRVVIAVGFIIALVAFLG-----CCGAWKENKCMLICFFAFLLLLLILEIVGGALAYNNKDKIENRLDKDILKAIENYPGKNEKS-----INDMQTKFKCCGADNYTDWQSNIK---MKNSSSVPDSCCKS-EKAGCGVGGVKD--PKDIYTKGCFTIIKGEIEKSLK-PIGGLAIAVLVIQLLGMVFALLLICRIKS 234
[Close]
BLAST of Tetraspanin vs. Ensembl Medaka
Match: tspan3b (tetraspanin-3 [Source:NCBI gene;Acc:101165292])

HSP 1 Score: 61.2326 bits (147), Expect = 3.168e-10
Identity = 42/134 (31.34%), Postives = 69/134 (51.49%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKK---YLKYAFLTLGLSITILAVLDTMFIL 1106
            K+ + V  ++ K+  +Y G N ++  S  I+ +Q Q HCCG++NYSD+ R   W         P+SCC+       N++ C  T T P     +GC + +  +LK+   Y+ +A LT   SI +L +L    +L
Sbjct:  110 KVEDQVNHSIQKVYNEYSGIN-SDAPSRAIDYVQKQLHCCGIHNYSDW-RNTHWFIESKNNSVPVSCCQP------NISNCTGTLTRPGDLYQEGCEALVVKKLKEIMMYVIWAALTFA-SIQMLGMLCACVVL 234
[Close]
BLAST of Tetraspanin vs. Ensembl Medaka
Match: tspan3a (tetraspanin-3 [Source:NCBI gene;Acc:101170088])

HSP 1 Score: 53.5286 bits (127), Expect = 1.101e-7
Identity = 32/114 (28.07%), Postives = 59/114 (51.75%), Query Frame = 3 
Query:  720 KIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKYAFLT 1055
            KI + V  ++  + E+Y G N +N +S  I+  Q Q  CCG++NY+D+ +  +W  +      P+SCC++       ++ C  + T P     +GC + +  +LK+ + Y   T
Sbjct:  110 KIEDEVNSSIVVVYEEYNGTN-SNSQSRAIDYTQRQLQCCGIHNYTDWLKT-RWYEKTKNNSVPISCCKT------TVSGCTGSLTHPEDLFKEGCEALVVKKLKEIMMYVIWT 215
[Close]
BLAST of Tetraspanin vs. Ensembl Medaka
Match: tspan3a (tetraspanin-3 [Source:NCBI gene;Acc:101170088])

HSP 1 Score: 52.7582 bits (125), Expect = 1.540e-7
Identity = 32/117 (27.35%), Postives = 59/117 (50.43%), Query Frame = 3 
Query:  711 GNQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPS--LTKGCYSSIKPQLKKYLKYAFLT 1055
            G   I + V  ++  + E+Y G N+ N +S  I+  Q Q  CCG++NY+D+ +  +W  +      P+SCC++       ++ C  + T P     +GC + +  +LK+ + Y   T
Sbjct:   82 GLTTIEDEVNSSIVVVYEEYNGTNS-NSQSRAIDYTQRQLQCCGIHNYTDWLKT-RWYEKTKNNSVPISCCKT------TVSGCTGSLTHPEDLFKEGCEALVVKKLKEIMMYVIWT 190
[Close]
BLAST of Tetraspanin vs. Ensembl Medaka
Match: tspan7 (tetraspanin 6 [Source:NCBI gene;Acc:101163337])

HSP 1 Score: 48.521 bits (114), Expect = 4.413e-6
Identity = 25/89 (28.09%), Postives = 43/89 (48.31%), Query Frame = 3 
Query:  771 EGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKYL 1037
              YN TN  S+ ++ IQ    CCG+ NY+D+  A  ++ + +    PLSCC+      V + +  D       T GC++ +   ++  L
Sbjct:  131 RSYNGTNSLSVALDTIQRTLRCCGVKNYTDWKEAPYFISKGI----PLSCCKDNGNCTVEIMKDLDKAGKEVFTTGCFALVTTVMESNL 215
[Close]
BLAST of Tetraspanin vs. Ensembl Medaka
Match: tspan7 (tetraspanin 6 [Source:NCBI gene;Acc:101163337])

HSP 1 Score: 48.1358 bits (113), Expect = 5.670e-6
Identity = 25/87 (28.74%), Postives = 43/87 (49.43%), Query Frame = 3 
Query:  777 YNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKYL 1037
            YN TN  S+ ++ IQ    CCG+ NY+D+  A  ++ + +    PLSCC+      V + +  D       T GC++ +   ++  L
Sbjct:  133 YNGTNSLSVALDTIQRTLRCCGVKNYTDWKEAPYFISKGI----PLSCCKDNGNCTVEIMKDLDKAGKEVFTTGCFALVTTVMESNL 215
[Close]
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000005510.1 (SMESG000005510.1)

HSP 1 Score: 439.499 bits (1129), Expect = 4.826e-155
Identity = 237/238 (99.58%), Postives = 238/238 (100.00%), Query Frame = 3 
Query:  417 MNSIMPKIYDLSGHMKKLLQNIHVNANGIKSFEEVISANSATIGAIAFSIGVLFIAIAILGYLSLCCCGAKCFXXXXXXXXXXXXXXXXXXXXXYGAQGNQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKYLKYAFLTLGLSITILAVLDTMFILFLIFENRS 1130
            MNSIMPKIYDLSGHMKKLLQNIHVNANGIKSFEEVISANSATIGAIAFSIGVLFIAIAILGYLSLCCCGAKCFIYVIIAIINALLVSLLVIAIVYGAQGNQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKYLKYAF+TLGLSITILAVLDTMFILFLIFENRS
Sbjct:   37 MNSIMPKIYDLSGHMKKLLQNIHVNANGIKSFEEVISANSATIGAIAFSIGVLFIAIAILGYLSLCCCGAKCFIYVIIAIINALLVSLLVIAIVYGAQGNQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKFPLSCCESREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKYLKYAFVTLGLSITILAVLDTMFILFLIFENRS 274
[Close]
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000041117.1 (SMESG000041117.1)

HSP 1 Score: 134.035 bits (336), Expect = 4.559e-36
Identity = 79/245 (32.24%), Postives = 140/245 (57.14%), Query Frame = 3 
Query:  417 MNSIMPKIYDLSGHMKKLLQN--IHVNANGIKSFEEVISANSATIGAIAFSIGVLFIAIAILGYLSLCCCGAKCFXXXXXXXXXXXXXXXXXXXXXYGAQGNQKIFNYVEKNLY-KLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWV-------GRKLGFKFPLSCCESREMRYVNLA---ECKDTWTSPSLTKGCYSSIKPQLKKYLKYAFLTLGLSITIL--AVLDTMFIL 1106
            MN  +  IYDL   ++ L++   I  +A+G+ S    +  N+++IG IAF  G + IA++++G +++CCC  K  + VI   +    ++L+ +   YG+    +I   +   +   +I KY   N+T  +S++INVIQ  A+CCG+YNY+D++ A  +         +     FPLSCC+ ++   +++A    C  T TS  +  GCY+ ++P L+KYL Y F T+G++I IL  A++ T+ ++
Sbjct:   37 MNYFLKNIYDLQAQIQNLIEKLGIKTDASGVISL---VQQNASSIGIIAFIAGCILIALSVIGIVAVCCCTLKLILIVICIFLICFTLALIGVVAYYGSNPTDRILVPLTNTIQNDIISKYRDMNSTEPQSMLINVIQITANCCGVYNYTDYNNATNFYRQATASDNKNYPIIFPLSCCDYKQDFTIDMASRDSCIKTQTSSKMNMGCYTRLEPDLRKYLGYCFATIGITIGILVAAIIVTIILI 278
[Close]
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000056555.1 (SMESG000056555.1)

HSP 1 Score: 69.707 bits (169), Expect = 3.968e-13
Identity = 39/114 (34.21%), Postives = 61/114 (53.51%), Query Frame = 3 
Query:  735 VEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKW---------VGRK-----LGFKFPLSCCESREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKY 1034
            VE  L  +I KYEG+ + N ES +INV+    +CCG  NY DF+ + K+          G K     +   +P+SCC+ +E ++VN  EC +   S +  KGC +S+   ++ Y
Sbjct:  122 VEGILKDMIIKYEGFFSANPESFLINVMNIGLNCCGAINYQDFNASVKFNRTYSGTFTYGGKTETYNVQLNYPISCCQFKEFQFVNSRECIENKGSNNKDKGCVTSLFDSIQNY 235
[Close]
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000056558.1 (SMESG000056558.1)

HSP 1 Score: 65.4698 bits (158), Expect = 9.524e-12
Identity = 51/192 (26.56%), Postives = 96/192 (50.00%), Query Frame = 3 
Query:  537 ATIGAIAFSIGVLFIAIAILGYLSLCCCGAKCFXXXXXXXXXXXXXXXXXXXXXYGA--QGNQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKF---------------PLSCCESREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKYLKYAFLTLG 1061
             +I  I   +G +   ++I+G  S+CC  ++C I + ++II+A+    +++ I+Y A  + ++K+ N + KN+   I KY G+  T  ES ++N +    +CCG   Y DF+ +  +  R     F               P+SCC+ ++ ++VN+  C    T  +   GC  S+   +K+YL   +  +G
Sbjct:   58 VSISVILIIVGSILFVLSIIGMCSVCC-KSQCLIIIYLSIISAVFAIQIIVVIIYAANPEMSKKMINDLLKNM---IAKYRGFTNTEPESFLVNFLNIFMNCCGATGYQDFNSSINF-NRTFSGNFTYGSKSQTYNVYLYYPISCCKFKDFQFVNMNGCLQNQTDNNKDSGCTESLFNSIKEYLIKVYQVVG 244
[Close]
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000056558.1 (SMESG000056558.1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.153e-11
Identity = 51/192 (26.56%), Postives = 96/192 (50.00%), Query Frame = 3 
Query:  537 ATIGAIAFSIGVLFIAIAILGYLSLCCCGAKCFXXXXXXXXXXXXXXXXXXXXXYGA--QGNQKIFNYVEKNLYKLIEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKF---------------PLSCCESREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKYLKYAFLTLG 1061
             +I  I   +G +   ++I+G  S+CC  ++C I + ++II+A+    +++ I+Y A  + ++K+ N + KN+   I KY G+  T  ES ++N +    +CCG   Y DF+ +  +  R     F               P+SCC+ ++ ++VN+  C    T  +   GC  S+   +K+YL   +  +G
Sbjct:   77 VSISVILIIVGSILFVLSIIGMCSVCC-KSQCLIIIYLSIISAVFAIQIIVVIIYAANPEMSKKMINDLLKNM---IAKYRGFTNTEPESFLVNFLNIFMNCCGATGYQDFNSSINF-NRTFSGNFTYGSKSQTYNVYLYYPISCCKFKDFQFVNMNGCLQNQTDNNKDSGCTESLFNSIKEYLIKVYQVVG 263
The following BLAST results are available for this feature:
BLAST of Tetraspanin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
TSPAN32.366e-830.00tetraspanin 3 [Source:HGNC Symbol;Acc:HGNC:17752][more]
TSPAN32.564e-831.53tetraspanin 3 [Source:HGNC Symbol;Acc:HGNC:17752][more]
TSPAN35.877e-830.00tetraspanin 3 [Source:HGNC Symbol;Acc:HGNC:17752][more]
TSPAN39.168e-829.20tetraspanin 3 [Source:HGNC Symbol;Acc:HGNC:17752][more]
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BLAST of Tetraspanin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tspan3a3.378e-829.10tetraspanin 3a [Source:ZFIN;Acc:ZDB-GENE-030131-77... [more]
tspan3a3.378e-829.10tetraspanin 3a [Source:ZFIN;Acc:ZDB-GENE-030131-77... [more]
tspan3a3.378e-829.10tetraspanin 3a [Source:ZFIN;Acc:ZDB-GENE-030131-77... [more]
tspan3b4.352e-729.20tetraspanin 3b [Source:ZFIN;Acc:ZDB-GENE-070424-42... [more]
tspan3b4.352e-729.20tetraspanin 3b [Source:ZFIN;Acc:ZDB-GENE-070424-42... [more]
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BLAST of Tetraspanin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
lrrk22.041e-828.57leucine-rich repeat kinase 2 [Source:Xenbase;Acc:X... [more]
lrrk22.687e-828.80leucine-rich repeat kinase 2 [Source:Xenbase;Acc:X... [more]
fam110d5.111e-627.68family with sequence similarity 110 member D [Sour... [more]
gpr885.584e-629.46G protein-coupled receptor 88 [Source:Xenbase;Acc:... [more]
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BLAST of Tetraspanin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Tspan32.077e-830.91tetraspanin 3 [Source:MGI Symbol;Acc:MGI:1928098][more]
Tspan32.116e-830.91tetraspanin 3 [Source:MGI Symbol;Acc:MGI:1928098][more]
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BLAST of Tetraspanin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q9QY33|TSN3_MOUSE1.453e-730.91Tetraspanin-3 OS=Mus musculus OX=10090 GN=Tspan3 P... [more]
sp|Q3SZR9|TSN3_BOVIN1.916e-730.00Tetraspanin-3 OS=Bos taurus OX=9913 GN=TSPAN3 PE=2... [more]
sp|Q5RE11|TSN3_PONAB2.822e-730.00Tetraspanin-3 OS=Pongo abelii OX=9601 GN=TSPAN3 PE... [more]
sp|O60637|TSN3_HUMAN2.822e-730.00Tetraspanin-3 OS=Homo sapiens OX=9606 GN=TSPAN3 PE... [more]
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BLAST of Tetraspanin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A419PZD41.035e-1024.04Tetraspanin-6 (Fragment) OS=Clonorchis sinensis OX... [more]
A0A099ZMK93.082e-934.43Tetraspanin (Fragment) OS=Tinamus guttatus OX=9482... [more]
A0A075AI315.047e-926.46Uncharacterized protein OS=Opisthorchis viverrini ... [more]
R7VAV17.114e-923.36Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A074ZGR19.085e-922.71Uncharacterized protein OS=Opisthorchis viverrini ... [more]
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BLAST of Tetraspanin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tspan361.648e-830.08tetraspanin 36 [Source:NCBI gene;Acc:437021][more]
tspan3a1.952e-831.82tetraspanin 3 [Source:NCBI gene;Acc:103046031][more]
tspan3a2.809e-831.82tetraspanin 3 [Source:NCBI gene;Acc:103046031][more]
tspan3a3.168e-831.82tetraspanin 3 [Source:NCBI gene;Acc:103046031][more]
tspan367.025e-828.70tetraspanin 36 [Source:NCBI gene;Acc:437021][more]
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BLAST of Tetraspanin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tspan4a2.664e-729.71tetraspanin 4a [Source:ZFIN;Acc:ZDB-GENE-040718-38... [more]
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BLAST of Tetraspanin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO398473.545e-1231.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Tetraspanin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tspan3b3.168e-1031.34tetraspanin-3 [Source:NCBI gene;Acc:101165292][more]
tspan3a1.101e-728.07tetraspanin-3 [Source:NCBI gene;Acc:101170088][more]
tspan3a1.540e-727.35tetraspanin-3 [Source:NCBI gene;Acc:101170088][more]
tspan74.413e-628.09tetraspanin 6 [Source:NCBI gene;Acc:101163337][more]
tspan75.670e-628.74tetraspanin 6 [Source:NCBI gene;Acc:101163337][more]
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BLAST of Tetraspanin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000005510.14.826e-15599.58SMESG000005510.1[more]
SMESG000041117.14.559e-3632.24SMESG000041117.1[more]
SMESG000056555.13.968e-1334.21SMESG000056555.1[more]
SMESG000056558.19.524e-1226.56SMESG000056558.1[more]
SMESG000056558.11.153e-1126.56SMESG000056558.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034552 ID=SMED30034552|Name=Tetraspanin|organism=Schmidtea mediterranea sexual|type=transcript|length=1266bp
CAAATTCGGCTATTTCGAAAATTTTAATCTGATAATACCGATGAAAAATA
CAGCTTGATTAAAATTAATTACTTTTGAAAGTCGTTTGAAAACACATGAG
TAATAAGTTCACAACCGTTTAATCCCGGTATATGCGATTTCTAATATGTT
GCTATTGACAACGGAGTTGGTTCGAATGCTCGACTAGTAGTTTTCGTATT
TGTCAAACGATTATGTAATGCGTGTGCCATCAATTTGATTGGTCGTATTT
TTTCATATTTAAACACGAGCTTCTTTATTACTTATTAATTTATAGATATT
TTTTTTAAATGTGTCTTGTTACAGGAATAATTAAATGTATTCTCGGAATT
GTAATTGGGATTATTCTGGTTTCCGGGTTGATTTTAATGGGCGTCGGTGG
AGTATTACTATGGGGCATGAATTCGATTATGCCGAAAATTTATGATTTAT
CAGGCCATATGAAAAAATTGCTGCAAAATATTCATGTTAATGCTAATGGT
ATAAAATCATTTGAAGAAGTTATTTCCGCAAATTCAGCTACAATAGGAGC
AATCGCATTTTCTATAGGGGTCCTATTCATTGCGATAGCGATTCTAGGAT
ATTTATCCCTGTGCTGTTGTGGAGCAAAATGTTTCATCTATGTGATAATT
GCAATAATTAACGCTCTACTGGTTTCACTCCTCGTCATAGCAATCGTTTA
TGGGGCTCAGGGTAACCAAAAGATATTCAATTATGTGGAAAAGAATCTTT
ATAAACTGATTGAAAAATACGAAGGATACAATGCGACAAATGTTGAATCC
TTGATAATAAATGTTATTCAGACCCAGGCTCACTGCTGCGGCTTGTACAA
TTATTCTGATTTTGACAGAGCTGAAAAGTGGGTCGGTCGCAAACTTGGGT
TCAAGTTTCCATTAAGTTGTTGCGAATCCAGAGAAATGAGATACGTCAAT
CTCGCGGAATGCAAAGACACTTGGACCTCTCCATCGTTAACCAAAGGTTG
TTATTCATCCATTAAACCTCAGTTAAAAAAATACCTCAAATATGCCTTCC
TCACATTGGGATTGTCCATAACTATTCTTGCGGTTTTGGATACGATGTTC
ATTTTATTTTTGATATTTGAAAATCGAAGTTGATATGATATACAATTTTA
TACTGATTGTGGGATTAATTATTAACTAATATTTTCTGGGCATTATGATT
ATTTTATAGATACTTATTATGCCAAAGATACTTATTATACAAGCCTTTTA
AATGGATATATAACTG
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protein sequence of SMED30034552-orf-1

>SMED30034552-orf-1 ID=SMED30034552-orf-1|Name=SMED30034552-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=275bp
MCLVTGIIKCILGIVIGIILVSGLILMGVGGVLLWGMNSIMPKIYDLSGH
MKKLLQNIHVNANGIKSFEEVISANSATIGAIAFSIGVLFIAIAILGYLS
LCCCGAKCFIYVIIAIINALLVSLLVIAIVYGAQGNQKIFNYVEKNLYKL
IEKYEGYNATNVESLIINVIQTQAHCCGLYNYSDFDRAEKWVGRKLGFKF
PLSCCESREMRYVNLAECKDTWTSPSLTKGCYSSIKPQLKKYLKYAFLTL
GLSITILAVLDTMFILFLIFENRS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR018499Tetraspanin/Peripherin
IPR008952Tetraspanin_EC2_sf
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018499Tetraspanin/PeripherinPFAMPF00335Tetraspanincoord: 8..261
e-value: 1.9E-18
score: 67.1
IPR008952Tetraspanin, EC2 domain superfamilyGENE3DG3DSA:1.10.1450.10coord: 132..242
e-value: 4.2E-7
score: 31.7
IPR008952Tetraspanin, EC2 domain superfamilySUPERFAMILYSSF48652Tetraspanincoord: 137..241
NoneNo IPR availablePANTHERPTHR19282:SF120TETRASPANIN-36coord: 65..255
NoneNo IPR availablePANTHERPTHR19282TETRASPANINcoord: 65..255
NoneNo IPR availableTMHMMTMhelixcoord: 79..101
NoneNo IPR availableTMHMMTMhelixcoord: 248..270
NoneNo IPR availableTMHMMTMhelixcoord: 13..35
NoneNo IPR availableTMHMMTMhelixcoord: 108..130