SMED30034542

Overview
NameSMED30034542
Smed IDSMED30034542
Length (bp)1545
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30034542 (SMED30034542) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30034542 (SMED30034542) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30034542

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30034542 dd_Smed_v4_24687_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30034542 dd_Smed_v4_24687_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30034542 vs. Ensembl Human
Match: GTF2IRD2B (GTF2I repeat domain containing 2B [Source:HGNC Symbol;Acc:HGNC:33125])

HSP 1 Score: 77.0258 bits (188), Expect = 3.312e-26
Identity = 46/131 (35.11%), Postives = 80/131 (61.07%), Query Frame = -1 
Query:  667 CCLFVAKSL---KRLVDYFLLLLICIKNY*FSSSNGLS*ILF---LSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 1041
            CC+   +SL   K  +D+  ++ + +K+  +  S GL+   F   L +L+     +LY+TE++WLS+G VLKRF+E L +I  F+ S+ K +P+L+  +W   LAFL  +T  LN+ N+ +QG  +++ +M
Sbjct:  652 CCIIHPESLCAQKLKMDH--VMDVVVKSVNWICSRGLNHSEFTTLLYELDSQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSIDWIRDLAFLVDMTMHLNALNISLQGHSQIVTQM 780

HSP 2 Score: 50.0618 bits (118), Expect = 3.312e-26
Identity = 32/111 (28.83%), Postives = 54/111 (48.65%), Query Frame = -2 
Query:  318 VKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFY 650
            ++AF  KL +    + + N  H P T  L+++N              K+  EFQ R  + + +E ++ +F +PFS  I++V    QME+ +LQ    LK  +    +P FY
Sbjct:  784 IRAFLAKLCLWETHLTRNNLAHFP-TLKLVSRNESDGLNYIPKIAELKT--EFQKRLSDFKLYESELTLFSSPFSTKIDSVHEELQMEVIDLQCNTVLKTKYDKVGIPEFY 891

HSP 3 Score: 32.3426 bits (72), Expect = 3.312e-26
Identity = 11/28 (39.29%), Postives = 20/28 (71.43%), Query Frame = -1 
Query:  217 THPNLRNHALKMVTIIGSTFICEHTFLI 300
            ++P  ++H  K++++ GST+ICE  F I
Sbjct:  897 SYPKYKHHCAKILSMFGSTYICEQLFSI 924
[Close]
BLAST of SMED30034542 vs. Ensembl Human
Match: GTF2IRD2B (GTF2I repeat domain containing 2B [Source:HGNC Symbol;Acc:HGNC:33125])

HSP 1 Score: 76.6406 bits (187), Expect = 4.106e-26
Identity = 45/131 (34.35%), Postives = 80/131 (61.07%), Query Frame = -1 
Query:  667 CCLFVAKSL---KRLVDYFLLLLICIKNY*FSSSNGLS*ILFLS---KLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 1041
            CC+   +SL   K  +D+  ++ + +K+  +  S GL+   F +   +L+     +LY+TE++WLS+G VLKRF+E L +I  F+ S+ K +P+L+  +W   LAFL  +T  LN+ N+ +QG  +++ +M
Sbjct:  819 CCIIHPESLCAQKLKMDH--VMDVVVKSVNWICSRGLNHSEFTTLLYELDSQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSIDWIRDLAFLVDMTMHLNALNISLQGHSQIVTQM 947

HSP 2 Score: 50.0618 bits (118), Expect = 4.106e-26
Identity = 32/111 (28.83%), Postives = 54/111 (48.65%), Query Frame = -2 
Query:  318 VKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFY 650
            ++AF  KL +    + + N  H P T  L+++N              K+  EFQ R  + + +E ++ +F +PFS  I++V    QME+ +LQ    LK  +    +P FY
Sbjct:  951 IRAFLAKLCLWETHLTRNNLAHFP-TLKLVSRNESDGLNYIPKIAELKT--EFQKRLSDFKLYESELTLFSSPFSTKIDSVHEELQMEVIDLQCNTVLKTKYDKVGIPEFY 1058

HSP 3 Score: 32.3426 bits (72), Expect = 4.106e-26
Identity = 11/28 (39.29%), Postives = 20/28 (71.43%), Query Frame = -1 
Query:  217 THPNLRNHALKMVTIIGSTFICEHTFLI 300
            ++P  ++H  K++++ GST+ICE  F I
Sbjct: 1064 SYPKYKHHCAKILSMFGSTYICEQLFSI 1091
[Close]
BLAST of SMED30034542 vs. Ensembl Human
Match: GTF2IRD2 (GTF2I repeat domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30775])

HSP 1 Score: 76.6406 bits (187), Expect = 1.443e-25
Identity = 46/131 (35.11%), Postives = 80/131 (61.07%), Query Frame = -1 
Query:  667 CCLFVAKSL---KRLVDYFLLLLICIKNY*FSSSNGLS*ILF---LSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 1041
            CC+   +SL   K  +D+  ++ + +K+  +  S GL+   F   L +L+     +LY+TE++WLS+G VLKRF+E L +I  F+ S+ K +P+L+  +W   LAFL  +T  LN+ N+ +QG  +++ +M
Sbjct:  652 CCIIHPESLCAQKLKMDH--VMDVVVKSVNWICSRGLNHSEFTTLLYELDSQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSIDWIRDLAFLVDMTMHLNALNISLQGHSQIVTQM 780

HSP 2 Score: 47.7506 bits (112), Expect = 1.443e-25
Identity = 32/113 (28.32%), Postives = 55/113 (48.67%), Query Frame = -2 
Query:  318 VKAFAVKLGILIKDVKKENFCHLPITQNLLAKNH--WFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFY 650
            ++AF  KL +    + + N  H P T  L ++N     ++  K   +      EFQ R  + + +E ++ +F +PFS  I++V    QME+ +LQ    LK  +    +P FY
Sbjct:  784 IRAFLAKLCLWETHLTRNNLAHFP-TLKLASRNESDGLNYIPKIAELQ----TEFQKRLSDFKLYESELTLFSSPFSTKIDSVHEELQMEVIDLQCNTVLKTKYDKVGIPEFY 891

HSP 3 Score: 32.3426 bits (72), Expect = 1.443e-25
Identity = 11/28 (39.29%), Postives = 20/28 (71.43%), Query Frame = -1 
Query:  217 THPNLRNHALKMVTIIGSTFICEHTFLI 300
            ++P  ++H  K++++ GST+ICE  F I
Sbjct:  897 SYPKYKHHCAKILSMFGSTYICEQLFSI 924
[Close]
BLAST of SMED30034542 vs. Ensembl Human
Match: GTF2IRD2 (GTF2I repeat domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30775])

HSP 1 Score: 76.6406 bits (187), Expect = 1.691e-25
Identity = 45/131 (34.35%), Postives = 80/131 (61.07%), Query Frame = -1 
Query:  667 CCLFVAKSL---KRLVDYFLLLLICIKNY*FSSSNGLS*ILFLS---KLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 1041
            CC+   +SL   K  +D+  ++ + +K+  +  S GL+   F +   +L+     +LY+TE++WLS+G VLKRF+E L +I  F+ S+ K +P+L+  +W   LAFL  +T  LN+ N+ +QG  +++ +M
Sbjct:  814 CCIIHPESLCAQKLKMDH--VMDVVVKSVNWICSRGLNHSEFTTLLYELDSQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSIDWIRDLAFLVDMTMHLNALNISLQGHSQIVTQM 942

HSP 2 Score: 47.7506 bits (112), Expect = 1.691e-25
Identity = 32/113 (28.32%), Postives = 55/113 (48.67%), Query Frame = -2 
Query:  318 VKAFAVKLGILIKDVKKENFCHLPITQNLLAKNH--WFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFY 650
            ++AF  KL +    + + N  H P T  L ++N     ++  K   +      EFQ R  + + +E ++ +F +PFS  I++V    QME+ +LQ    LK  +    +P FY
Sbjct:  946 IRAFLAKLCLWETHLTRNNLAHFP-TLKLASRNESDGLNYIPKIAELQ----TEFQKRLSDFKLYESELTLFSSPFSTKIDSVHEELQMEVIDLQCNTVLKTKYDKVGIPEFY 1053

HSP 3 Score: 32.3426 bits (72), Expect = 1.691e-25
Identity = 11/28 (39.29%), Postives = 20/28 (71.43%), Query Frame = -1 
Query:  217 THPNLRNHALKMVTIIGSTFICEHTFLI 300
            ++P  ++H  K++++ GST+ICE  F I
Sbjct: 1059 SYPKYKHHCAKILSMFGSTYICEQLFSI 1086
[Close]
BLAST of SMED30034542 vs. Ensembl Human
Match: EPM2AIP1 (EPM2A interacting protein 1 [Source:HGNC Symbol;Acc:HGNC:19735])

HSP 1 Score: 54.299 bits (129), Expect = 8.468e-11
Identity = 37/150 (24.67%), Postives = 74/150 (49.33%), Query Frame = -2 
Query:  225 HVKAFAVKLGILIKDVKKENFCHLPITQNLL-------AKNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYASSSPRHIPISGTMHSKW*QSLAALLSANTL 653
            H+  F VKL +  + ++++N    P  + ++        ++       ++  +  +  KEF+  FK+LRF ++D+++F NPF+   E      ++EL++LQ   +L + + +  L  FYA  S    PI   +  K    +A+L  +N +
Sbjct:  411 HICTFEVKLNLFQRHIEEKNLTDFPALREVVDELKQQNKEDEKIFDPDRYQMVICRLQKEFERHFKDLRFIKKDLELFSNPFNFKPEYAPISVRVELTKLQANTNLWNEYRIKDLGQFYAGLSAESYPIIKGVACK----VASLFDSNQI 556

HSP 2 Score: 32.7278 bits (73), Expect = 8.468e-11
Identity = 15/59 (25.42%), Postives = 29/59 (49.15%), Query Frame = -1 
Query:  694 WLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQ 870
            WL +G+ LK  + L  +++ FL+S        +D +W     FL  + E L   + +++
Sbjct:  341 WLRRGKTLKLIFSLRKEMEAFLVSVGATTVHFSDKQWLCDFGFLVDIMEHLRELSEELR 399
[Close]
BLAST of SMED30034542 vs. Ensembl Zebrafish
Match: AL928808.1 (pep chromosome:GRCz11:20:17257101:17259573:-1 gene:ENSDARG00000101333.2 transcript:ENSDART00000166397.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL928808.1)

HSP 1 Score: 80.8777 bits (198), Expect = 1.128e-15
Identity = 40/81 (49.38%), Postives = 55/81 (67.90%), Query Frame = -1 
Query:  655 DVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM*HTV 897
            D+L+H  VRWLS+G VL RF+ELL  +K FL  K+K+ PEL+D +W   LAFL  V   LN  NV +QGK K++ ++  +V
Sbjct:  339 DLLFHCAVRWLSRGHVLSRFFELLNPVKLFLAEKHKEYPELHDPQWISDLAFLVDVLHYLNGLNVDLQGKLKMLPDLVQSV 419
[Close]
BLAST of SMED30034542 vs. Ensembl Zebrafish
Match: CR392001.3 (pep chromosome:GRCz11:8:38963323:38965260:-1 gene:ENSDARG00000117159.1 transcript:ENSDART00000181495.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR392001.3)

HSP 1 Score: 63.1586 bits (152), Expect = 6.533e-11
Identity = 32/77 (41.56%), Postives = 44/77 (57.14%), Query Frame = -1 
Query:  667 DVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 897
            D+L H +VRWLS+  VL+RF   L  +K FL SK+   P+L D EW   L F+  +T  LN  N  +Q +G    +M
Sbjct:  332 DLLLHNKVRWLSKDEVLRRFVACLEHVKTFLKSKDLIYPQLEDTEWLEKLHFMVDMTSHLNKLNESLQVRGNTALQM 408

HSP 2 Score: 23.8682 bits (50), Expect = 6.533e-11
Identity = 23/107 (21.50%), Postives = 44/107 (41.12%), Query Frame = -2 
Query:  363 VKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYW----IHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTI-------YQMELSELQNCD 650
            V +F  KL +  +DV++    H P  +    ++H  H     Y     +  ++   F+ RF E R  +  +     P  ID   + T         +ME++++ + D
Sbjct:  412 VLSFERKLTVFARDVQRGTLSHFPSLRE-FKESHQDHTINGDYLHNAIVDMQTA--FESRFCEFRKEKMTLSFPVTPLEIDPSLLSTFPGIIQADLEMEMADISDKD 515
[Close]
BLAST of SMED30034542 vs. Ensembl Xenopus
Match: ENSXETT00000016563.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:4:37379994:37381838:-1 gene:ENSXETG00000009412.1 transcript:ENSXETT00000016563.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.8474 bits (146), Expect = 4.994e-20
Identity = 33/90 (36.67%), Postives = 52/90 (57.78%), Query Frame = -1 
Query:  667 FLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLL---SKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 927
            FL +L+    D+L HT VRWLS G+ L RF+ L  +I  +L      +  +  L D  +   LAFLT +T+ LNS N+ +QG+ + + ++
Sbjct:  330 FLEELDAAYGDLLLHTNVRWLSAGKCLVRFFALRKEIHLYLSDIKCDSYLLEHLTDVSFLTALAFLTDITQFLNSLNLNLQGRDQNVSQL 419

HSP 2 Score: 40.817 bits (94), Expect = 4.994e-20
Identity = 32/104 (30.77%), Postives = 44/104 (42.31%), Query Frame = -2 
Query:  369 SHVKAFAVKLGILIKDVKKENFCHLP----ITQNLLAKNHWFHFQ----TKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQN 656
            SH+ AF  KL +L   + K +  H      + Q +       +F          IH     EF  RF++    E   ++F NP + DI   DT Y MEL ELQ 
Sbjct:  421 SHIAAFRNKLHLLKSFLAKNDLTHFESCCELFQEMKGNGVILNFSQFVPKMDILIH-----EFNTRFQDFEGMEASFKLFNNPINTDIAAQDTKYHMELCELQG 519

HSP 3 Score: 36.1946 bits (82), Expect = 4.994e-20
Identity = 15/34 (44.12%), Postives = 22/34 (64.71%), Query Frame = -1 
Query:  223 FLCLLLSETHPNLRNHALKMVTIIGSTFICEHTF 324
            F  LL  E +P LR+  ++M ++ GST+ICE  F
Sbjct:  533 FFKLLSQEQYPILRDFGMRMASMFGSTYICEKAF 566
[Close]
BLAST of SMED30034542 vs. Ensembl Xenopus
Match: spag1 (sperm associated antigen 1 [Source:Xenbase;Acc:XB-GENE-853609])

HSP 1 Score: 60.8474 bits (146), Expect = 3.336e-9
Identity = 33/90 (36.67%), Postives = 52/90 (57.78%), Query Frame = -1 
Query:  667 FLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLL---SKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 927
            FL +L+    D+L HT VRWLS G+ L RF+ L  +I  +L      +  +  L D  +   LAFLT +T+ LNS N+ +QG+ + + ++
Sbjct:  330 FLEELDAAYGDLLLHTNVRWLSAGKCLVRFFALRKEIHLYLSDIKCDSYLLEHLTDVSFLTALAFLTDITQFLNSLNLNLQGRDQNVSQL 419
[Close]
BLAST of SMED30034542 vs. Ensembl Xenopus
Match: ENSXETT00000010836.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:7:63865670:63866962:-1 gene:ENSXETG00000001503.1 transcript:ENSXETT00000010836.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.0618 bits (118), Expect = 1.842e-6
Identity = 26/65 (40.00%), Postives = 37/65 (56.92%), Query Frame = -1 
Query:  673 LSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLIC 867
            +    V  RF+ LLP+I  F   K++   EL D +W   LAFLT  T  LN+ N+++QG+ K  C
Sbjct:  113 VKDAMVAVRFHYLLPEISQFSEEKDELYSELYDYQWLADLAFLTDKTSKLNNLNLELQGQDKTTC 177
[Close]
BLAST of SMED30034542 vs. Ensembl Mouse
Match: Gtf2ird2 (GTF2I repeat domain containing 2 [Source:MGI Symbol;Acc:MGI:2149780])

HSP 1 Score: 72.7886 bits (177), Expect = 1.164e-23
Identity = 31/76 (40.79%), Postives = 51/76 (67.11%), Query Frame = -1 
Query:  667 VLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 894
            +LYHT ++WL +G VL+RF+E L +I  F+ S+ K VP+L+  +W   LAFL  +T  LN+ +  +QG  +++ +M
Sbjct:  699 LLYHTALKWLGRGLVLRRFFESLEEIDSFMSSRGKPVPQLSSRDWILDLAFLVDMTTHLNTLDASLQGHSQIVTQM 774

HSP 2 Score: 45.4394 bits (106), Expect = 1.164e-23
Identity = 32/122 (26.23%), Postives = 63/122 (51.64%), Query Frame = -2 
Query:  297 VKAFAVKLGILIKDVKKENFCHLPITQNL-LAKNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYA---SSSPRH 650
            ++AF  KL +    + + N  H P  +++  +++   ++  K   +  K+  EFQ R  + +  E ++ +F +PFS  I++V    QME+ +LQ    L+  +    +P+FY    SS P++
Sbjct:  778 IRAFLAKLCLWETHLARNNLAHFPTLKSVSRSESDGLNYIPK--IVELKA--EFQRRLSDFKSCESELTLFSSPFSTTIDSVREELQMEVIDLQCNTVLRTKYDKVGVPDFYKHLWSSYPKY 895

HSP 3 Score: 31.5722 bits (70), Expect = 1.164e-23
Identity = 11/28 (39.29%), Postives = 18/28 (64.29%), Query Frame = -1 
Query:  217 THPNLRNHALKMVTIIGSTFICEHTFLI 300
            ++P  R+H  +M+++  ST ICE  F I
Sbjct:  891 SYPKYRSHCARMLSMFSSTHICEQLFSI 918
[Close]
BLAST of SMED30034542 vs. Ensembl Mouse
Match: Zmym6 (zinc finger, MYM-type 6 [Source:MGI Symbol;Acc:MGI:106505])

HSP 1 Score: 52.373 bits (124), Expect = 1.391e-6
Identity = 27/66 (40.91%), Postives = 40/66 (60.61%), Query Frame = -1 
Query:  691 HTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPE-LNDAEWKWHLAFLTGVTELLNSFNVQVQG 885
            + EVRWLS+GR+L R +EL  +I+ FL  K+  +    +D EW   LA+L  +  L+N  N  +QG
Sbjct:  899 NAEVRWLSRGRILTRLFELRHEIEIFLNQKHSDLARYFHDEEWIAKLAYLADIFSLINKLNSSLQG 964
[Close]
BLAST of SMED30034542 vs. Ensembl Mouse
Match: Zmym6 (zinc finger, MYM-type 6 [Source:MGI Symbol;Acc:MGI:106505])

HSP 1 Score: 52.373 bits (124), Expect = 1.449e-6
Identity = 27/66 (40.91%), Postives = 40/66 (60.61%), Query Frame = -1 
Query:  691 HTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPE-LNDAEWKWHLAFLTGVTELLNSFNVQVQG 885
            + EVRWLS+GR+L R +EL  +I+ FL  K+  +    +D EW   LA+L  +  L+N  N  +QG
Sbjct:  991 NAEVRWLSRGRILTRLFELRHEIEIFLNQKHSDLARYFHDEEWIAKLAYLADIFSLINKLNSSLQG 1056
[Close]
BLAST of SMED30034542 vs. Ensembl Mouse
Match: Zbed5 (zinc finger, BED type containing 5 [Source:MGI Symbol;Acc:MGI:1919220])

HSP 1 Score: 50.447 bits (119), Expect = 4.570e-6
Identity = 35/95 (36.84%), Postives = 54/95 (56.84%), Query Frame = -1 
Query:  691 FSSSNGLS*ILFL---SKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPE--LNDAEWKWHLAFLTGVTELLNSFNVQVQG 960
            F  ++ L+  LFL   S L+  +K +L HTE RWLS+G+VLKR +EL  ++K F   K  +  E   +D      +A+L  +  +LN  N+ +QG
Sbjct:  462 FVKASSLNSRLFLQLCSDLDEPSKTLLLHTEGRWLSRGKVLKRIFELRDELKMFFNQKAIRQFEALFSDNSALQKVAYLVDIFTILNELNLSLQG 556
[Close]
BLAST of SMED30034542 vs. Ensembl Mouse
Match: Epm2aip1 (EPM2A (laforin) interacting protein 1 [Source:MGI Symbol;Acc:MGI:1925031])

HSP 1 Score: 49.6766 bits (117), Expect = 8.968e-6
Identity = 26/71 (36.62%), Postives = 41/71 (57.75%), Query Frame = -2 
Query:  288 KEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYASSSPRHIPI 500
            K+F+  FK+LRF ++D+++F NPFS   E      ++EL++LQ    L + + +  L  FYA  S    PI
Sbjct:  470 KDFERHFKDLRFIKKDLELFSNPFSFKPEYAPISVRVELTKLQANTDLWNEYRVKDLGQFYAGLSGEAYPI 540
[Close]
BLAST of SMED30034542 vs. UniProt/SwissProt
Match: sp|A4IFA3|GT2D2_BOVIN (General transcription factor II-I repeat domain-containing protein 2 OS=Bos taurus OX=9913 GN=GTF2IRD2 PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.802e-26
Identity = 44/129 (34.11%), Postives = 76/129 (58.91%), Query Frame = -1 
Query:  667 CCLFVAKSL---KRLVDYFLLLLICIKNY*FSSS-NGLS*ILFLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 1041
            CC+   +SL   K  +D+ + +++   N+  S   N       L +L+     +LY+TE++WLS+G VLKRF+E L +I  F+ S+ K +P+L+  +W   LAFL  +T  LN+ N+ +QG  +++ +M
Sbjct:  653 CCIIHPESLCAQKLKMDHIMSVVVNAVNWICSRGLNHSEFTTLLYELDCQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSQDWIKDLAFLVDMTMHLNTLNISLQGHSQIVTQM 781

HSP 2 Score: 52.7582 bits (125), Expect = 3.802e-26
Identity = 31/111 (27.93%), Postives = 56/111 (50.45%), Query Frame = -2 
Query:  318 VKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFY 650
            ++AF  KL +    + + N  H P  +++ ++N           +  K+  EFQ R  + + +E ++ +F +PFS+ IE+V    QME+ +LQ    LK  +    +P FY
Sbjct:  785 IRAFLAKLCLWETHLARNNLAHFPTLKSV-SRNESDGLNYIPKIVELKT--EFQKRLSDFKLYESELTLFSSPFSMKIESVQEALQMEVIDLQCNTVLKTKYDKVGIPEFY 892

HSP 3 Score: 30.4166 bits (67), Expect = 3.802e-26
Identity = 11/28 (39.29%), Postives = 19/28 (67.86%), Query Frame = -1 
Query:  217 THPNLRNHALKMVTIIGSTFICEHTFLI 300
            ++P  + H  K++++ GST+ICE  F I
Sbjct:  898 SYPKYKIHCAKILSMFGSTYICEQLFSI 925
[Close]
BLAST of SMED30034542 vs. UniProt/SwissProt
Match: sp|Q6EKJ0|GTD2B_HUMAN (General transcription factor II-I repeat domain-containing protein 2B OS=Homo sapiens OX=9606 GN=GTF2IRD2B PE=1 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.456e-25
Identity = 46/131 (35.11%), Postives = 80/131 (61.07%), Query Frame = -1 
Query:  667 CCLFVAKSL---KRLVDYFLLLLICIKNY*FSSSNGLS*ILF---LSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 1041
            CC+   +SL   K  +D+  ++ + +K+  +  S GL+   F   L +L+     +LY+TE++WLS+G VLKRF+E L +I  F+ S+ K +P+L+  +W   LAFL  +T  LN+ N+ +QG  +++ +M
Sbjct:  652 CCIIHPESLCAQKLKMDH--VMDVVVKSVNWICSRGLNHSEFTTLLYELDSQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSIDWIRDLAFLVDMTMHLNALNISLQGHSQIVTQM 780

HSP 2 Score: 50.0618 bits (118), Expect = 1.456e-25
Identity = 32/111 (28.83%), Postives = 54/111 (48.65%), Query Frame = -2 
Query:  318 VKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFY 650
            ++AF  KL +    + + N  H P T  L+++N              K+  EFQ R  + + +E ++ +F +PFS  I++V    QME+ +LQ    LK  +    +P FY
Sbjct:  784 IRAFLAKLCLWETHLTRNNLAHFP-TLKLVSRNESDGLNYIPKIAELKT--EFQKRLSDFKLYESELTLFSSPFSTKIDSVHEELQMEVIDLQCNTVLKTKYDKVGIPEFY 891

HSP 3 Score: 32.3426 bits (72), Expect = 1.456e-25
Identity = 11/28 (39.29%), Postives = 20/28 (71.43%), Query Frame = -1 
Query:  217 THPNLRNHALKMVTIIGSTFICEHTFLI 300
            ++P  ++H  K++++ GST+ICE  F I
Sbjct:  897 SYPKYKHHCAKILSMFGSTYICEQLFSI 924
[Close]
BLAST of SMED30034542 vs. UniProt/SwissProt
Match: sp|Q86UP8|GTD2A_HUMAN (General transcription factor II-I repeat domain-containing protein 2A OS=Homo sapiens OX=9606 GN=GTF2IRD2 PE=1 SV=3)

HSP 1 Score: 76.6406 bits (187), Expect = 6.346e-25
Identity = 46/131 (35.11%), Postives = 80/131 (61.07%), Query Frame = -1 
Query:  667 CCLFVAKSL---KRLVDYFLLLLICIKNY*FSSSNGLS*ILF---LSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 1041
            CC+   +SL   K  +D+  ++ + +K+  +  S GL+   F   L +L+     +LY+TE++WLS+G VLKRF+E L +I  F+ S+ K +P+L+  +W   LAFL  +T  LN+ N+ +QG  +++ +M
Sbjct:  652 CCIIHPESLCAQKLKMDH--VMDVVVKSVNWICSRGLNHSEFTTLLYELDSQYGSLLYYTEIKWLSRGLVLKRFFESLEEIDSFMSSRGKPLPQLSSIDWIRDLAFLVDMTMHLNALNISLQGHSQIVTQM 780

HSP 2 Score: 47.7506 bits (112), Expect = 6.346e-25
Identity = 32/113 (28.32%), Postives = 55/113 (48.67%), Query Frame = -2 
Query:  318 VKAFAVKLGILIKDVKKENFCHLPITQNLLAKNH--WFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFY 650
            ++AF  KL +    + + N  H P T  L ++N     ++  K   +      EFQ R  + + +E ++ +F +PFS  I++V    QME+ +LQ    LK  +    +P FY
Sbjct:  784 IRAFLAKLCLWETHLTRNNLAHFP-TLKLASRNESDGLNYIPKIAELQ----TEFQKRLSDFKLYESELTLFSSPFSTKIDSVHEELQMEVIDLQCNTVLKTKYDKVGIPEFY 891

HSP 3 Score: 32.3426 bits (72), Expect = 6.346e-25
Identity = 11/28 (39.29%), Postives = 20/28 (71.43%), Query Frame = -1 
Query:  217 THPNLRNHALKMVTIIGSTFICEHTFLI 300
            ++P  ++H  K++++ GST+ICE  F I
Sbjct:  897 SYPKYKHHCAKILSMFGSTYICEQLFSI 924
[Close]
BLAST of SMED30034542 vs. UniProt/SwissProt
Match: sp|Q99NI3|GT2D2_MOUSE (General transcription factor II-I repeat domain-containing protein 2 OS=Mus musculus OX=10090 GN=Gtf2ird2 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 7.067e-23
Identity = 31/76 (40.79%), Postives = 51/76 (67.11%), Query Frame = -1 
Query:  667 VLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 894
            +LYHT ++WL +G VL+RF+E L +I  F+ S+ K VP+L+  +W   LAFL  +T  LN+ +  +QG  +++ +M
Sbjct:  699 LLYHTALKWLGRGLVLRRFFESLEEIDSFMSSRGKPVPQLSSRDWILDLAFLVDMTTHLNTLDASLQGHSQIVTQM 774

HSP 2 Score: 45.4394 bits (106), Expect = 7.067e-23
Identity = 32/122 (26.23%), Postives = 63/122 (51.64%), Query Frame = -2 
Query:  297 VKAFAVKLGILIKDVKKENFCHLPITQNL-LAKNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYA---SSSPRH 650
            ++AF  KL +    + + N  H P  +++  +++   ++  K   +  K+  EFQ R  + +  E ++ +F +PFS  I++V    QME+ +LQ    L+  +    +P+FY    SS P++
Sbjct:  778 IRAFLAKLCLWETHLARNNLAHFPTLKSVSRSESDGLNYIPK--IVELKA--EFQRRLSDFKSCESELTLFSSPFSTTIDSVREELQMEVIDLQCNTVLRTKYDKVGVPDFYKHLWSSYPKY 895

HSP 3 Score: 31.5722 bits (70), Expect = 7.067e-23
Identity = 11/28 (39.29%), Postives = 18/28 (64.29%), Query Frame = -1 
Query:  217 THPNLRNHALKMVTIIGSTFICEHTFLI 300
            ++P  R+H  +M+++  ST ICE  F I
Sbjct:  891 SYPKYRSHCARMLSMFSSTHICEQLFSI 918
[Close]
BLAST of SMED30034542 vs. UniProt/SwissProt
Match: sp|Q7L775|EPMIP_HUMAN (EPM2A-interacting protein 1 OS=Homo sapiens OX=9606 GN=EPM2AIP1 PE=1 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 3.723e-10
Identity = 37/150 (24.67%), Postives = 74/150 (49.33%), Query Frame = -2 
Query:  225 HVKAFAVKLGILIKDVKKENFCHLPITQNLL-------AKNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYASSSPRHIPISGTMHSKW*QSLAALLSANTL 653
            H+  F VKL +  + ++++N    P  + ++        ++       ++  +  +  KEF+  FK+LRF ++D+++F NPF+   E      ++EL++LQ   +L + + +  L  FYA  S    PI   +  K    +A+L  +N +
Sbjct:  411 HICTFEVKLNLFQRHIEEKNLTDFPALREVVDELKQQNKEDEKIFDPDRYQMVICRLQKEFERHFKDLRFIKKDLELFSNPFNFKPEYAPISVRVELTKLQANTNLWNEYRIKDLGQFYAGLSAESYPIIKGVACK----VASLFDSNQI 556

HSP 2 Score: 32.7278 bits (73), Expect = 3.723e-10
Identity = 15/59 (25.42%), Postives = 29/59 (49.15%), Query Frame = -1 
Query:  694 WLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQ 870
            WL +G+ LK  + L  +++ FL+S        +D +W     FL  + E L   + +++
Sbjct:  341 WLRRGKTLKLIFSLRKEMEAFLVSVGATTVHFSDKQWLCDFGFLVDIMEHLRELSEELR 399
[Close]
BLAST of SMED30034542 vs. TrEMBL
Match: A0A401QEL1 (Uncharacterized protein OS=Scyliorhinus torazame OX=75743 GN=scyTo_0024192 PE=4 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 1.152e-47
Identity = 94/143 (65.73%), Postives = 113/143 (79.02%), Query Frame = -1 
Query:  499 FLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM*HTVTCESICS*IRYPHQRREKRKFLPSSHNSKPASQKPLVSFPNKTLLDSLEKLQ 927
            FLS+LNV ++DVLYHTEVRWLS+GRVLKRFY+LLPQI  FLLSKNKKVPELNDAEWKWHLAFLT VTELLN+FN+Q+QGKGKLIC+M   V    +   +     + E    LP++ N    ++KPL++FPNKT +DSLEKLQ
Sbjct:  321 FLSELNVEHEDVLYHTEVRWLSRGRVLKRFYDLLPQITTFLLSKNKKVPELNDAEWKWHLAFLTDVTELLNNFNMQLQGKGKLICDMHSHVKAFEVKLDLLIKQVKEENFCHLPTTQN--LLAEKPLIAFPNKTCVDSLEKLQ 461

HSP 2 Score: 160.614 bits (405), Expect = 5.534e-39
Identity = 84/127 (66.14%), Postives = 93/127 (73.23%), Query Frame = -2 
Query:  291 CDIQSHVKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSCK-EFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYASSSPRHIP 668
            CD+ SHVKAF VKL +LIK VK+ENFCHLP TQNLLA+     F  K      +  + EFQ RFKEL  HE DIQ+FRNPFS+DIENVDTIYQMEL+ELQNCDSLKDAF  SSL NFYAS      P
Sbjct:  405 CDMHSHVKAFEVKLDLLIKQVKEENFCHLPTTQNLLAEKPLIAFPNKTCVDSLEKLQTEFQFRFKELHLHEWDIQLFRNPFSVDIENVDTIYQMELAELQNCDSLKDAFKSSSLHNFYASLPSETYP 531

HSP 3 Score: 53.1434 bits (126), Expect = 3.569e-8
Identity = 23/34 (67.65%), Postives = 27/34 (79.41%), Query Frame = -1 
Query:  223 FLCLLLSETHPNLRNHALKMVTIIGSTFICEHTF 324
            F   L SET+P+LRNHALKM TI GST++CE TF
Sbjct:  521 FYASLPSETYPHLRNHALKMATIFGSTYVCEQTF 554

HSP 4 Score: 35.039 bits (79), Expect = 3.569e-8
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = -3 
Query:  128 AVINMEPDIDHFISKKLANSS 190
            AV NMEPDIDH IS+K A+SS
Sbjct:  580 AVTNMEPDIDHLISQKQAHSS 600
[Close]
BLAST of SMED30034542 vs. TrEMBL
Match: L7MZH3 (Uncharacterized protein OS=Anolis carolinensis OX=28377 PE=4 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 6.467e-47
Identity = 90/144 (62.50%), Postives = 112/144 (77.78%), Query Frame = -1 
Query:  496 FLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM*HTVTCESICS*IRYPHQRREKRKFLPSSHNSKPASQKPLVSFPNKTLLDSLEKLQR 927
            FLS+LN   +DVLYHTEVRWLS+GRVLK FY+LLPQI  FLLSKNK+VPELNDAEWKWHLAFLT +TELLNSFNVQ+QGKGKLIC+M   V    +   +     + E    LP++ +   +++KPL++FPNKT +DSLE+LQ+
Sbjct:  228 FLSELNAAYEDVLYHTEVRWLSRGRVLKHFYDLLPQITAFLLSKNKEVPELNDAEWKWHLAFLTDITELLNSFNVQLQGKGKLICDMQSHVKAFEVKLGLLIKQVKEENFCHLPTTQS--LSAEKPLIAFPNKTCVDSLERLQK 369

HSP 2 Score: 169.859 bits (429), Expect = 5.446e-43
Identity = 87/127 (68.50%), Postives = 95/127 (74.80%), Query Frame = -2 
Query:  291 CDIQSHVKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSC-KEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYASSSPRHIP 668
            CD+QSHVKAF VKLG+LIK VK+ENFCHLP TQ+L A+     F  K      +   KEFQ RFKEL  HEQDIQ+FRNPFSIDIENVDTIYQMEL+ELQNCDSLKDAF   SLPNFYAS      P
Sbjct:  312 CDMQSHVKAFEVKLGLLIKQVKEENFCHLPTTQSLSAEKPLIAFPNKTCVDSLERLQKEFQFRFKELHLHEQDIQLFRNPFSIDIENVDTIYQMELAELQNCDSLKDAFKPRSLPNFYASLPSETYP 438

HSP 3 Score: 55.0694 bits (131), Expect = 7.528e-8
Identity = 24/34 (70.59%), Postives = 27/34 (79.41%), Query Frame = -1 
Query:  223 FLCLLLSETHPNLRNHALKMVTIIGSTFICEHTF 324
            F   L SET+PNLRNHALKM TI GST++CE TF
Sbjct:  428 FYASLPSETYPNLRNHALKMATIFGSTYVCEQTF 461

HSP 4 Score: 31.9574 bits (71), Expect = 7.528e-8
Identity = 14/21 (66.67%), Postives = 18/21 (85.71%), Query Frame = -3 
Query:  128 AVINMEPDIDHFISKKLANSS 190
            A+ NMEPDID+ IS+K A+SS
Sbjct:  487 AMTNMEPDIDYLISQKQAHSS 507
[Close]
BLAST of SMED30034542 vs. TrEMBL
Match: G1KF58 (Uncharacterized protein OS=Anolis carolinensis OX=28377 PE=4 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 3.504e-46
Identity = 90/144 (62.50%), Postives = 112/144 (77.78%), Query Frame = -1 
Query:  496 FLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM*HTVTCESICS*IRYPHQRREKRKFLPSSHNSKPASQKPLVSFPNKTLLDSLEKLQR 927
            FLS+LN   +DVLYHTEVRWLS+GRVLK FY+LLPQI  FLLSKNK+VPELNDAEWKWHLAFLT +TELLNSFNVQ+QGKGKLIC+M   V    +   +     + E    LP++ +   +++KPL++FPNKT +DSLE+LQ+
Sbjct:  324 FLSELNAAYEDVLYHTEVRWLSRGRVLKHFYDLLPQITAFLLSKNKEVPELNDAEWKWHLAFLTDITELLNSFNVQLQGKGKLICDMQSHVKAFEVKLGLLIKQVKEENFCHLPTTQS--LSAEKPLIAFPNKTCVDSLERLQK 465

HSP 2 Score: 169.474 bits (428), Expect = 2.988e-42
Identity = 87/127 (68.50%), Postives = 95/127 (74.80%), Query Frame = -2 
Query:  291 CDIQSHVKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSC-KEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYASSSPRHIP 668
            CD+QSHVKAF VKLG+LIK VK+ENFCHLP TQ+L A+     F  K      +   KEFQ RFKEL  HEQDIQ+FRNPFSIDIENVDTIYQMEL+ELQNCDSLKDAF   SLPNFYAS      P
Sbjct:  408 CDMQSHVKAFEVKLGLLIKQVKEENFCHLPTTQSLSAEKPLIAFPNKTCVDSLERLQKEFQFRFKELHLHEQDIQLFRNPFSIDIENVDTIYQMELAELQNCDSLKDAFKPRSLPNFYASLPSETYP 534

HSP 3 Score: 55.4546 bits (132), Expect = 2.598e-8
Identity = 24/34 (70.59%), Postives = 27/34 (79.41%), Query Frame = -1 
Query:  223 FLCLLLSETHPNLRNHALKMVTIIGSTFICEHTF 324
            F   L SET+PNLRNHALKM TI GST++CE TF
Sbjct:  524 FYASLPSETYPNLRNHALKMATIFGSTYVCEQTF 557

HSP 4 Score: 33.113 bits (74), Expect = 2.598e-8
Identity = 15/21 (71.43%), Postives = 18/21 (85.71%), Query Frame = -3 
Query:  128 AVINMEPDIDHFISKKLANSS 190
            AV NMEPDID+ IS+K A+SS
Sbjct:  583 AVTNMEPDIDYLISQKQAHSS 603
[Close]
BLAST of SMED30034542 vs. TrEMBL
Match: G3VUN9 (Uncharacterized protein OS=Sarcophilus harrisii OX=9305 PE=4 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 1.187e-43
Identity = 80/127 (62.99%), Postives = 91/127 (71.65%), Query Frame = -2 
Query:  312 RASSSVKCDIQSHVKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSC-KEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYAS 689
            +    + CD QS VKAF VKLG+LIK  K+ENFCHLP TQNLL +     F  K      +   KEFQ R KEL  HEQDIQ+F NPF+IDIENVDTIYQ+EL+ELQNCDSLKDAF  SSL +FYAS
Sbjct:    4 QGKGKLICDTQSPVKAFEVKLGLLIKQEKEENFCHLPTTQNLLEEKPLIAFPNKTCVDSLEKLQKEFQFRLKELHLHEQDIQLFHNPFTIDIENVDTIYQVELAELQNCDSLKDAFKSSSLHHFYAS 130

HSP 2 Score: 42.3578 bits (98), Expect = 1.187e-43
Identity = 18/34 (52.94%), Postives = 24/34 (70.59%), Query Frame = -1 
Query:  223 FLCLLLSETHPNLRNHALKMVTIIGSTFICEHTF 324
            F   L SE + +L+NHA+KM TI GS ++CE TF
Sbjct:  127 FYASLPSEKYHHLKNHAVKMATIFGSAYVCEQTF 160

HSP 3 Score: 32.7278 bits (73), Expect = 1.187e-43
Identity = 15/22 (68.18%), Postives = 18/22 (81.82%), Query Frame = -3 
Query:  125 AVINMEPDIDHFISKKLANSSN 190
            AV NM PDIDH IS+K A+SS+
Sbjct:  186 AVTNMVPDIDHLISQKQAHSSH 207
[Close]
BLAST of SMED30034542 vs. TrEMBL
Match: A0A5F8GNQ2 (Uncharacterized protein OS=Monodelphis domestica OX=13616 PE=4 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 4.635e-43
Identity = 84/125 (67.20%), Postives = 93/125 (74.40%), Query Frame = -2 
Query:  297 CDIQSHVKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSC-KEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYASSSPRH 668
            CDIQ HVKAF VKLG+LIK V+ ENFCHL +TQNLLA+     F  +      +   KEFQ RFKEL  HEQDIQ+FRN FSIDIENV TIYQMEL++LQNCDS KDAF  SSL NFYAS  PRH
Sbjct:  406 CDIQPHVKAFEVKLGLLIKQVE-ENFCHLLLTQNLLAEKPLIAFPKETCVDSLEKLQKEFQFRFKELHLHEQDIQLFRNSFSIDIENVYTIYQMELAKLQNCDSPKDAFKSSSLLNFYASLPPRH 529

HSP 2 Score: 51.6026 bits (122), Expect = 4.635e-43
Identity = 20/25 (80.00%), Postives = 22/25 (88.00%), Query Frame = -1 
Query:  223 HPNLRNHALKMVTIIGSTFICEHTF 297
            HPNLRNHALKM TI GST++CE TF
Sbjct:  529 HPNLRNHALKMATIFGSTYVCEQTF 553

HSP 3 Score: 157.918 bits (398), Expect = 3.072e-38
Identity = 84/156 (53.85%), Postives = 104/156 (66.67%), Query Frame = -1 
Query:  496 LFLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM*HTVTCESICS*IRYPHQ-----------RREKRKFLPSSHNSKPASQKPLVSFPNKTLLDSLEKLQR 930
            LFLS+LNV  +DVLYHTEV WLSQGRVL+ FY+LLPQI  F+ SKNK+VPELNDAEWKW+LAFL  VTELLNSFNVQ+QGK KLIC++               PH            ++ +  F          ++KPL++FP +T +DSLEKLQ+
Sbjct:  321 LFLSELNVAYEDVLYHTEVLWLSQGRVLRHFYDLLPQITAFMFSKNKEVPELNDAEWKWYLAFLIDVTELLNSFNVQLQGKRKLICDI--------------QPHVKAFEVKLGLLIKQVEENFCHLLLTQNLLAEKPLIAFPKETCVDSLEKLQK 462
[Close]
BLAST of SMED30034542 vs. Ensembl Cavefish
Match: ENSAMXT00000037411.1 (pep primary_assembly:Astyanax_mexicanus-2.0:13:18144228:18145385:-1 gene:ENSAMXG00000033115.1 transcript:ENSAMXT00000037411.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 143.28 bits (360), Expect = 1.221e-37
Identity = 76/144 (52.78%), Postives = 98/144 (68.06%), Query Frame = -1 
Query:  499 FLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM*HTVTCESICS*IRYPHQRR-EKRKFLPSSHNSKPASQKPLVSFPNKTLLDSLEKLQ 927
            FLS+L     DVLY+TEVRWLS+GRVL+RFYELLP+I  FL SK+K VPEL D EWKWHLAFLT VTE+LNS N+Q+QG+GKLIC+M              Y H +  E +  LP++ N   ++  P V FP +  +++LE L+
Sbjct:  118 FLSELESAYGDVLYYTEVRWLSRGRVLRRFYELLPEINAFLHSKDKTVPELLDPEWKWHLAFLTDVTEMLNSLNLQLQGQGKLICDM--------------YSHIKAFELKLALPATQNL--SADNPAVPFPTEKCVEALEMLK 245

HSP 2 Score: 90.1225 bits (222), Expect = 1.341e-26
Identity = 52/127 (40.94%), Postives = 72/127 (56.69%), Query Frame = -2 
Query:  312 RASSSVKCDIQSHVKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSCK-EFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYAS 689
            +    + CD+ SH+KAF +KL              LP TQNL A N    F T+      +  K EF +RF+EL  + ++I++F+NPF  DI+     YQ EL+ELQNCD LKD F  +SL +FYA+
Sbjct:  195 QGQGKLICDMYSHIKAFELKLA-------------LPATQNLSADNPAVPFPTEKCVEALEMLKGEFGVRFRELHVNAKEIRLFQNPFVADIDEAQPSYQFELAELQNCDVLKDVFKPNSLIDFYAA 308

HSP 3 Score: 46.2098 bits (108), Expect = 1.341e-26
Identity = 18/34 (52.94%), Postives = 26/34 (76.47%), Query Frame = -1 
Query:  223 FLCLLLSETHPNLRNHALKMVTIIGSTFICEHTF 324
            F   L ++T+PN++ HA+KM T+ GST+ICE TF
Sbjct:  305 FYAALPNDTYPNIKKHAMKMSTLFGSTYICEQTF 338

HSP 4 Score: 23.483 bits (49), Expect = 1.341e-26
Identity = 11/21 (52.38%), Postives = 14/21 (66.67%), Query Frame = -3 
Query:  128 AVINMEPDIDHFISKKLANSS 190
            AV  MEPDI+   S+  A+SS
Sbjct:  364 AVTRMEPDIELLPSQMQAHSS 384
[Close]
BLAST of SMED30034542 vs. Ensembl Cavefish
Match: ENSAMXT00000044284.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:30442742:30446828:1 gene:ENSAMXG00000031237.1 transcript:ENSAMXT00000044284.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 103.99 bits (258), Expect = 2.671e-25
Identity = 54/114 (47.37%), Postives = 77/114 (67.54%), Query Frame = -2 
Query:  291 LGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSC-KEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYASSSPRHIP 629
            LG+L+K VK++NFCH P TQ   A+     F T+      +   +EFQ+RF+EL  H +DI++F++PF+ DI+ +D IYQ+EL+ELQN D+LKDAF  S+L +FYAS      P
Sbjct:   34 LGLLLKHVKEKNFCHFPTTQKRAAEVPSLGFPTEKCAEALEILQREFQVRFQELHAHAKDIRLFQSPFAADIDCIDPIYQLELAELQNFDNLKDAFKSSTLVDFYASLPGETYP 147

HSP 2 Score: 50.8322 bits (120), Expect = 6.189e-7
Identity = 23/42 (54.76%), Postives = 27/42 (64.29%), Query Frame = -1 
Query:  199 FLCLLLSETHPNLRNHALKMVTIIGSTFICEHTFLIHTCITC 324
            F   L  ET+PNL+ HALKM TI GST+ICE TF     + C
Sbjct:  137 FYASLPGETYPNLKKHALKMTTIFGSTYICEQTFSRMKQLKC 178
[Close]
BLAST of SMED30034542 vs. Ensembl Cavefish
Match: ENSAMXT00000030525.1 (pep primary_assembly:Astyanax_mexicanus-2.0:1:12992682:12994541:1 gene:ENSAMXG00000035573.1 transcript:ENSAMXT00000030525.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 75.0998 bits (183), Expect = 2.852e-25
Identity = 36/74 (48.65%), Postives = 50/74 (67.57%), Query Frame = -1 
Query:  676 DVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLI 897
            DVLY TEVRWLS+G +LKRF+EL  ++K F+      V  L+D +W   LAFL  +T  LN  N ++QG+G+L+
Sbjct:  379 DVLYFTEVRWLSRGNILKRFFELRAEVKAFMEKDGVAVTVLSDPKWLMDLAFLVDITHELNVLNKKLQGQGQLV 452

HSP 2 Score: 57.7658 bits (138), Expect = 2.852e-25
Identity = 36/123 (29.27%), Postives = 59/123 (47.97%), Query Frame = -2 
Query:  285 HVKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYASSSPRHIPIS 653
            +V+AF+ KL +    + + NFCH P  + L+     F  + K+        +EF  RF + + H    Q+F +PFS D+++   + QMEL +LQ    L+  F   S          R +P+S
Sbjct:  458 NVRAFSAKLMLWKAQLSQTNFCHFPACKALMDAGTPFSGE-KYVDAILMLQEEFDHRFADFKTHRATFQIFADPFSFDVQDAPPVLQMELIDLQCNSELRAKFREVSGKADKLGQFLRELPLS 579

HSP 3 Score: 22.3274 bits (46), Expect = 2.852e-25
Identity = 8/26 (30.77%), Postives = 14/26 (53.85%), Query Frame = -1 
Query:  223 THPNLRNHALKMVTIIGSTFICEHTF 300
            + P L     + + + GST++CE  F
Sbjct:  579 SFPELSRMFKRTMCLFGSTYLCEKLF 604
[Close]
BLAST of SMED30034542 vs. Ensembl Cavefish
Match: ENSAMXT00000035253.1 (general transcription factor II-I repeat domain-containing protein 2B-like [Source:NCBI gene;Acc:111191579])

HSP 1 Score: 70.4774 bits (171), Expect = 1.961e-22
Identity = 37/93 (39.78%), Postives = 56/93 (60.22%), Query Frame = -1 
Query:  652 LFLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM*HTVT 930
            +FL +++  + DVLYHTEVRWLS+ R    F+EL   I  F+ SK   + EL+D +W    A L  +TE L   N ++QG+ ++I +M   +T
Sbjct:  320 MFLQEIDSEHGDVLYHTEVRWLSRSR----FFELREDIALFMQSKGNPLSELSDPKWLCDFAVLCDITEHLAQLNQKLQGRKQVITQMSDMIT 408

HSP 2 Score: 55.0694 bits (131), Expect = 1.961e-22
Identity = 35/122 (28.69%), Postives = 57/122 (46.72%), Query Frame = -2 
Query:  291 VKAFAVKLGILIKDVKKENFCHLPITQNLLA--KNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYASSSPRHIP 650
            + AF  KL + +   K++N  H P+ Q++ A     +   Q        K+  EF  RF + R  +    +F NPF+ D+       QMEL ELQ+   L+  F  +++ +FY    P  +P
Sbjct:  407 ITAFQRKLDLWMCQAKQDNLAHFPVCQSMSALFPGTFSCAQLTTKLSRLKT--EFDRRFSDFRGQQSGFAIFANPFTTDVCTAPQHLQMELIELQSDSGLRAKFQDAAIQDFYRLLPPGLMP 526
[Close]
BLAST of SMED30034542 vs. Ensembl Cavefish
Match: ENSAMXT00000037725.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:29162452:29166863:1 gene:ENSAMXG00000037303.1 transcript:ENSAMXT00000037725.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 88.5817 bits (218), Expect = 3.745e-18
Identity = 45/98 (45.92%), Postives = 63/98 (64.29%), Query Frame = -1 
Query:  634 FLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM*HTVTCESICS 927
            FL  ++    DV+YHTEVRWLS+G VLKRF+ L  +IK FL  K K    + + +W   LAFLT +T LLN  N+++QGK +LIC +      E++C+
Sbjct:  510 FLEDISAAYGDVIYHTEVRWLSRGNVLKRFFALRNEIKLFLEQKGKDTLVMENTDWIADLAFLTDLTGLLNELNLKLQGKDRLICHL-----FEAVCA 602

HSP 2 Score: 54.299 bits (129), Expect = 2.521e-7
Identity = 50/197 (25.38%), Postives = 82/197 (41.62%), Query Frame = -2 
Query:  264 NVSMSYFH-RSKIFCFLKTKKYQSSMMQNGNGTLPF*QV*QSYSTVSMCKFKERASSSVKCDIQSHVKAFAVKLGILIKDVKKENFCHLPITQNLLA-KNH-WFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYASSSPRHIPISGTMHSKW 845
            NV   +F  R++I  FL+ K   + +M+N +       +      ++    K +    + C +   V AF +KL +    +KK N  H P  Q   A KN+ W    ++H  +       F  RF + R  +  +Q+  +PFS+D E V    Q E+ EL+   +++       L  FY S      P       KW
Sbjct:  534 NVLKRFFALRNEIKLFLEQKGKDTLVMENTDWIADLAFLTDLTGLLNELNLKLQGKDRLICHLFEAVCAFEMKLNLFATQLKKGNLTHFPTCQEAFAHKNYNW----SRHSCVLEDLKLAFSARFGQFRNEQATLQLLADPFSVDTETVPGELQPEIIELKCSTAMRTKHREMPLLEFYQSLDREQFPNLFANAQKW 726
[Close]
BLAST of SMED30034542 vs. Ensembl Medaka
Match: ENSORLT00000044825.1 (general transcription factor II-I repeat domain-containing protein 2-like [Source:NCBI gene;Acc:111949247])

HSP 1 Score: 85.8853 bits (211), Expect = 5.778e-31
Identity = 40/87 (45.98%), Postives = 60/87 (68.97%), Query Frame = -1 
Query:  667 FLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 927
            FL +++   KD+LYH EVRWLS+G VLKRF+EL   I +FL S ++      D  W + +AF+  +T+LLN+ NV++QGK ++I E+
Sbjct:  285 FLDEVDAQYKDILYHQEVRWLSRGTVLKRFFELRQLIAEFLSSASRDTQIPTDKRWIFDVAFMVDITDLLNNLNVKLQGKEQIITEL 371

HSP 2 Score: 54.299 bits (129), Expect = 5.778e-31
Identity = 39/122 (31.97%), Postives = 62/122 (50.82%), Query Frame = -2 
Query:  291 HVKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDS-LKDAFSLSSLPNFYASSSPRHIP 653
            H+KAF +KL +L + +   NF H P  +++   N       ++  +     KEF LRF + +    D+++F  PFS+  ++V    QMEL E Q CD+ L+  F    L +FY   S +  P
Sbjct:  374 HIKAFQMKLQLLCRHLSAGNFAHFPSLRDV---NVEVDRLPEYGELLSNLNKEFNLRFVDFKKTAGDMELFSQPFSVSPDSVPEHLQMELIEFQ-CDTELRRKFVSLPLRDFYPHVSKQRYP 491

HSP 3 Score: 34.6538 bits (78), Expect = 5.778e-31
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = -1 
Query:  223 ETHPNLRNHALKMVTIIGSTFICEHTF 303
            + +P +R +A  M+++ GST+ICE TF
Sbjct:  488 QRYPQMRKNAQVMLSLFGSTYICEQTF 514
[Close]
BLAST of SMED30034542 vs. Ensembl Medaka
Match: ENSORLT00000027093.1 (general transcription factor II-I repeat domain-containing protein 2B-like [Source:NCBI gene;Acc:111947639])

HSP 1 Score: 81.6481 bits (200), Expect = 3.189e-26
Identity = 39/89 (43.82%), Postives = 55/89 (61.80%), Query Frame = -1 
Query:  667 ILFLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 933
            + FL +  + + D+ YH  VRWLS G+VLK  ++L  QI+DF + K   VPEL+D +W   L F   VT L+N  NV++Q KG  + EM
Sbjct:  304 VAFLEENEIEHGDIRYHCTVRWLSLGKVLKSVWDLRGQIQDFCVDKGHDVPELSDEDWVADLGFAVDVTALMNQLNVKLQSKGLFVHEM 392

HSP 2 Score: 56.9954 bits (136), Expect = 3.189e-26
Identity = 42/125 (33.60%), Postives = 63/125 (50.40%), Query Frame = -2 
Query:  291 DIQSHVKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYASSSPRHIP 665
            ++ S VKAF  KL  L   VK  N  HLP  +   AK    H Q K+  +      EF  RF++ +  E ++ V  +PF+  +++  +  QMEL ELQ+   L + F   SL +FY+S    + P
Sbjct:  391 EMYSAVKAFMRKLQFLSSQVKDNNLTHLPTLKE--AKRPEGHLQ-KYSTMLEALHAEFSRRFQDFKTLESEMHVVSSPFNCIVDDAPSEVQMELIELQSDMLLAERFRSVSLLDFYSSLKKENFP 512
[Close]
BLAST of SMED30034542 vs. Ensembl Medaka
Match: ENSORLT00000033014.1 (general transcription factor II-I repeat domain-containing protein 2 [Source:NCBI gene;Acc:101164918])

HSP 1 Score: 74.7146 bits (182), Expect = 7.069e-26
Identity = 34/77 (44.16%), Postives = 49/77 (63.64%), Query Frame = -1 
Query:  667 DVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 897
            DV+YH++VRWLS+G  L  FY L  +I  FL SK + +PEL+D  W   LAFL  +T  LN+ N  +QG+   + ++
Sbjct:  331 DVVYHSDVRWLSRGSALHHFYFLKEEIGQFLASKGQPMPELSDPVWLADLAFLVDITRHLNALNTSLQGENAAVSQL 407

HSP 2 Score: 50.447 bits (119), Expect = 7.069e-26
Identity = 35/103 (33.98%), Postives = 53/103 (51.46%), Query Frame = -2 
Query:  363 SHVKAFAVKLGILIKDV--KKENFCHLPITQNLLAK--NHWFHFQTKHYWIHWKS-CKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCD 656
            SH+KAF  KL +  K +  +  N  H P+ Q ++     +    Q + Y     S  +EFQ RF++    E++I +F +PFS+  +      QMEL ELQ CD
Sbjct:  409 SHIKAFGTKLQLFQKHLLQRPPNPAHFPLLQEIMTSLSQNNVSAQMRKYAADMASLAEEFQQRFQDFAAIEKEITLFSSPFSVRPDEAPDHLQMELIELQ-CD 510

HSP 3 Score: 32.3426 bits (72), Expect = 7.069e-26
Identity = 11/24 (45.83%), Postives = 19/24 (79.17%), Query Frame = -1 
Query:  217 LRNHALKMVTIIGSTFICEHTFLI 288
            +R  A+KM+++ GST++CE TF +
Sbjct:  537 IRTFAMKMLSLFGSTYLCEKTFSV 560
[Close]
BLAST of SMED30034542 vs. Ensembl Medaka
Match: ENSORLT00000046355.1 (pep primary_assembly:ASM223467v1:21:4582475:4584322:1 gene:ENSORLG00000025904.1 transcript:ENSORLT00000046355.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 87.4261 bits (215), Expect = 8.856e-18
Identity = 43/88 (48.86%), Postives = 58/88 (65.91%), Query Frame = -1 
Query:  667 LFLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 930
            + L +++    D+L HTE+RWLS+GRVL RF  LL +IK+F+ SK + V  L D EW   LAFL  +T  LN  N Q+QGKGK + +M
Sbjct:  321 VLLEEMSAEYGDLLLHTEIRWLSRGRVLHRFLSLLREIKEFMQSKGEDVSLLEDTEWTLDLAFLVDITGKLNLLNCQLQGKGKTVVDM 408
[Close]
BLAST of SMED30034542 vs. Ensembl Medaka
Match: ENSORLT00000028655.1 (pep primary_assembly:ASM223467v1:8:18412528:18414615:1 gene:ENSORLG00000023148.1 transcript:ENSORLT00000028655.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 85.8853 bits (211), Expect = 2.022e-17
Identity = 51/139 (36.69%), Postives = 73/139 (52.52%), Query Frame = -1 
Query:  511 FLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM*HTVTCESICS*IRYPHQRREKRKFLPSSHNSKPASQKPLVSFPNKTLLDSL 927
            FL +L+    ++ YHTEVRWLSQG+VL+R +EL   I  F+++K K   EL D  +   +AFL  +T  LN  N+Q+QG+G++I +M  TV        +   H R+E     PS    K      +  FP     D L
Sbjct:  330 FLGELDTEYGELPYHTEVRWLSQGKVLQRCFELREGICLFIVNKGKDTTELRDETFLCEMAFLCDITSHLNVMNLQLQGRGRVISDMYSTVQVFKTKLSLWETHMRKENLSHFPSCQTMKEKLSTAV--FPTAQFADKL 466
[Close]
BLAST of SMED30034542 vs. Planmine SMEST
Match: SMESG000045419.1 (SMESG000045419.1)

HSP 1 Score: 49.2914 bits (116), Expect = 7.694e-16
Identity = 29/87 (33.33%), Postives = 44/87 (50.57%), Query Frame = -1 
Query:  667 FLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM 927
            FL +++    DV+Y++ VRWLS+G VL+R Y L                          LAF+  +T  LN  N+ +QGKGKL+ ++
Sbjct:  299 FLKEIDSEFNDVIYYSAVRWLSRGAVLRRKYYLTA---------------------IGSLAFMVDITTYLNELNLNLQGKGKLLADL 364

HSP 2 Score: 45.8246 bits (107), Expect = 7.694e-16
Identity = 37/117 (31.62%), Postives = 57/117 (48.72%), Query Frame = -2 
Query:  318 CDIQSHVKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFY 668
            CDI     AF  KL +L K + +EN  H    +++ A        T     H KS                +I++F NPF++DI +V    QME+ +LQ+ + LK+AF L ++ +FY
Sbjct:  366 CDIN----AFEAKLCLLKKHMIEENLTHFICCKSVPADGWQTQKGTFFMDFHNKS---------------SEIRLFLNPFNVDINDVPNELQMEILQLQHNEILKNAFLLENIQHFY 463

HSP 3 Score: 27.335 bits (59), Expect = 7.694e-16
Identity = 12/29 (41.38%), Postives = 17/29 (58.62%), Query Frame = -1 
Query:  223 LSETHPNLRNHALKMVTIIGSTFICEHTF 309
            L + +  L + A KM+   GST+ICE  F
Sbjct:  466 LPKQYEGLISFAKKMIVAFGSTYICEQEF 494
[Close]
BLAST of SMED30034542 vs. Planmine SMEST
Match: SMESG000011571.1 (SMESG000011571.1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.965e-13
Identity = 36/84 (42.86%), Postives = 51/84 (60.71%), Query Frame = -1 
Query:  676 FLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLI 927
            FL ++     ++L H +VRWLS+  VLK F  LLP+I+ FLL K    PEL + +W  +  F+  VT  LN  N+++QGKG  I
Sbjct:   89 FLVEMESEYANLLLHNKVRWLSRRNVLKLFSSLLPEIEVFLLEKGVHYPELTNNQWIQYCHFVVDVTSHLNQLNLKLQGKGNTI 172
[Close]
BLAST of SMED30034542 vs. Planmine SMEST
Match: SMESG000037770.1 (SMESG000037770.1)

HSP 1 Score: 62.3882 bits (150), Expect = 5.699e-10
Identity = 36/84 (42.86%), Postives = 49/84 (58.33%), Query Frame = -1 
Query:  685 FLSKLNVINKDVLYHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKV---PELNDAEWKWHLAFLTGVTELLNSFNVQVQGKG 927
            FL  L   + DVLYHT VRWLS G+VLKR + L  +I  FL  K+  V    ++  +EW    +F   + + LN  NV++QGKG
Sbjct:  828 FLEDLESDHSDVLYHTNVRWLSLGKVLKRVWNLKDEIVLFLEMKDITVEFTTQMKTSEWLSDFSFAVDILDKLNELNVKLQGKG 911
[Close]
BLAST of SMED30034542 vs. Planmine SMEST
Match: SMESG000080048.1 (SMESG000080048.1)

HSP 1 Score: 46.595 bits (109), Expect = 6.127e-10
Identity = 25/63 (39.68%), Postives = 38/63 (60.32%), Query Frame = -1 
Query:  670 ELLPQIKDFLLSKN--------KKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICE 834
            E +  +KD+ L+++         K+ EL D +W   L F+  +TE LNSFNV+ QG+GKLI +
Sbjct:   43 ETVSVLKDYNLNRHYISRYDSFAKIIELYDEQWLLDLCFIVKITEKLNSFNVEFQGEGKLIAD 105

HSP 2 Score: 36.1946 bits (82), Expect = 6.127e-10
Identity = 38/158 (24.05%), Postives = 61/158 (38.61%), Query Frame = -2 
Query:  222 RASSSVKCDIQSHVKAFAVKLGILIKDVKKENFCHLPITQNLLAKNHWFHFQTKHYWIHWKSCK-EFQLRFKELRFHEQDIQVFRNPFSIDIENVDTIYQMELSELQNCDSLKDAFSLSSLPNFYAS-SSPRHIPISGTMHSKW*QSLAALLSANTLF 689
            +    +  D    +++F  KL + IK +      + P+  +    N    F  + Y    +  K EFQ RF        ++ +F  PF +  + V   YQMEL +LQ  + LK  F+      FY    +    P   T+  K   +     S   LF
Sbjct:   97 QGEGKLIADCFQKIQSFIAKLDLWIKQLDNNVALYFPLRNDYEVVNGSKMF--RRYADILRELKFEFQRRFTVFDKFNTELALFLTPFHVKADEVQFEYQMELIDLQAGEGLKHWFNEMLKIEFYKKFITTESFPNLNTLALKIISAFPTTYSCEALF 252
[Close]
BLAST of SMED30034542 vs. Planmine SMEST
Match: SMESG000043468.1 (SMESG000043468.1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.623e-9
Identity = 30/83 (36.14%), Postives = 52/83 (62.65%), Query Frame = -1 
Query:  640 YHTEVRWLSQGRVLKRFYELLPQIKDFLLSKNKKVPELNDAEWKWHLAFLTGVTELLNSFNVQVQGKGKLICEM*HTVTCESI 888
            Y+TEVRWLS  ++LK F++L  +I  FL+ KN+ +   +D  W    +F+  +T+ L+  N+++QGK ++I     T TC+ +
Sbjct:  107 YNTEVRWLSCSKILKPFWDLKEEICPFLIPKNQDMTLSSDTAWLQGSSFMVDITKHLSDLNLKLQGKDQII-----TSTCDQV 184
The following BLAST results are available for this feature:
BLAST of SMED30034542 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GTF2IRD2B3.312e-2635.11GTF2I repeat domain containing 2B [Source:HGNC Sym... [more]
GTF2IRD2B4.106e-2634.35GTF2I repeat domain containing 2B [Source:HGNC Sym... [more]
GTF2IRD21.443e-2535.11GTF2I repeat domain containing 2 [Source:HGNC Symb... [more]
GTF2IRD21.691e-2534.35GTF2I repeat domain containing 2 [Source:HGNC Symb... [more]
EPM2AIP18.468e-1124.67EPM2A interacting protein 1 [Source:HGNC Symbol;Ac... [more]
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BLAST of SMED30034542 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034542 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034542 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
AL928808.11.128e-1549.38pep chromosome:GRCz11:20:17257101:17259573:-1 gene... [more]
CR392001.36.533e-1141.56pep chromosome:GRCz11:8:38963323:38965260:-1 gene:... [more]
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BLAST of SMED30034542 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSXETT00000016563.14.994e-2036.67pep primary_assembly:Xenopus_tropicalis_v9.1:4:373... [more]
spag13.336e-936.67sperm associated antigen 1 [Source:Xenbase;Acc:XB-... [more]
ENSXETT00000010836.11.842e-640.00pep primary_assembly:Xenopus_tropicalis_v9.1:7:638... [more]
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BLAST of SMED30034542 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Gtf2ird21.164e-2340.79GTF2I repeat domain containing 2 [Source:MGI Symbo... [more]
Zmym61.391e-640.91zinc finger, MYM-type 6 [Source:MGI Symbol;Acc:MGI... [more]
Zmym61.449e-640.91zinc finger, MYM-type 6 [Source:MGI Symbol;Acc:MGI... [more]
Zbed54.570e-636.84zinc finger, BED type containing 5 [Source:MGI Sym... [more]
Epm2aip18.968e-636.62EPM2A (laforin) interacting protein 1 [Source:MGI ... [more]
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BLAST of SMED30034542 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|A4IFA3|GT2D2_BOVIN3.802e-2634.11General transcription factor II-I repeat domain-co... [more]
sp|Q6EKJ0|GTD2B_HUMAN1.456e-2535.11General transcription factor II-I repeat domain-co... [more]
sp|Q86UP8|GTD2A_HUMAN6.346e-2535.11General transcription factor II-I repeat domain-co... [more]
sp|Q99NI3|GT2D2_MOUSE7.067e-2340.79General transcription factor II-I repeat domain-co... [more]
sp|Q7L775|EPMIP_HUMAN3.723e-1024.67EPM2A-interacting protein 1 OS=Homo sapiens OX=960... [more]
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BLAST of SMED30034542 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A401QEL11.152e-4765.73Uncharacterized protein OS=Scyliorhinus torazame O... [more]
L7MZH36.467e-4762.50Uncharacterized protein OS=Anolis carolinensis OX=... [more]
G1KF583.504e-4662.50Uncharacterized protein OS=Anolis carolinensis OX=... [more]
G3VUN91.187e-4362.99Uncharacterized protein OS=Sarcophilus harrisii OX... [more]
A0A5F8GNQ24.635e-4367.20Uncharacterized protein OS=Monodelphis domestica O... [more]
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BLAST of SMED30034542 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000037411.11.221e-3752.78pep primary_assembly:Astyanax_mexicanus-2.0:13:181... [more]
ENSAMXT00000044284.12.671e-2547.37pep primary_assembly:Astyanax_mexicanus-2.0:14:304... [more]
ENSAMXT00000030525.12.852e-2548.65pep primary_assembly:Astyanax_mexicanus-2.0:1:1299... [more]
ENSAMXT00000035253.11.961e-2239.78general transcription factor II-I repeat domain-co... [more]
ENSAMXT00000037725.13.745e-1845.92pep primary_assembly:Astyanax_mexicanus-2.0:14:291... [more]
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BLAST of SMED30034542 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034542 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034542 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30034542 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000044825.15.778e-3145.98general transcription factor II-I repeat domain-co... [more]
ENSORLT00000027093.13.189e-2643.82general transcription factor II-I repeat domain-co... [more]
ENSORLT00000033014.17.069e-2644.16general transcription factor II-I repeat domain-co... [more]
ENSORLT00000046355.18.856e-1848.86pep primary_assembly:ASM223467v1:21:4582475:458432... [more]
ENSORLT00000028655.12.022e-1736.69pep primary_assembly:ASM223467v1:8:18412528:184146... [more]
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BLAST of SMED30034542 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000045419.17.694e-1633.33SMESG000045419.1[more]
SMESG000011571.12.965e-1342.86SMESG000011571.1[more]
SMESG000037770.15.699e-1042.86SMESG000037770.1[more]
SMESG000080048.16.127e-1039.68SMESG000080048.1[more]
SMESG000043468.12.623e-936.14SMESG000043468.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034542 ID=SMED30034542|Name=SMED30034542|organism=Schmidtea mediterranea sexual|type=transcript|length=1545bp
TAATAAAAGAGATCAGGGGTCCTCAAACTTTTTCAACAGGGCGTCGGTAC
ACTGTCCCTCAGACCGTTGAACGGCTGGAATAAAGTTTAAAAAAATGGTT
TTGAAAATTTTTTATTTAAAAACAATTGGAACTATTGGCCAGTTTTTTGC
TAATAAAATGGTCAATGTCCGGTTCCATATTTATCACTGCTAGTCGTAAC
AAGTGATGCAAGTGTGTATCAAAAAAGTGTGTTCGCAGATAAAAGTGCTG
CCAATGATTGTTACCATTTTGAGTGCATGGTTCCTGAGATTGGGATGTGT
CTCGGAGAGGAGGAGGCATAGAAATTAGGAAGGCTGCTTAAACTGAACGC
GTCTTTTAGAGAGTCACAATTCTGCAATTCAGACAGTTCCATTTGGTAAA
TTGTATCTACATTCTCAATGTCAATAGAAAATGGGTTACGGAAAACCTGT
ATGTCCTGTTCATGGAAACGAAGCTCTTTAAATCTAAGTTGGAACTCTTT
GCAACTTTTCCAATGAATCCAATAATGTTTTGTTTGGAAATGAAACCAAT
GGTTTTTGGCTAGCAGGTTTTGAGTTATGGGAAGATGGCAGAAATTTTCT
TTTTTCACGTCTTTGATGAGGATACCTAATTTAACTGCAAATGCTTTCAC
ATGTGACTGTATGTCACATTTCACAGATGAGCTTGCCCTTTCCTTGAACT
TGCACATTGAAACTGTTGAGTAGCTCTGTTACACCTGTCAAAAAGGCAAG
GTGCCATTTCCATTCTGCATCATTGAGCTCTGGTACTTTTTTGTTTTTAG
AAAGCAGAAAATCTTTGATCTGTGGAAGTAACTCATAGAAACGTTTCAAC
ACTCTCCCTTGACTTAGCCAACGGACTTCTGTATGGTACAGAACATCTTT
ATTGATAACATTTAGCTTAGACAAAAATAATATTTAGCTCAGACCGTTTG
ATGATGAAAACTAATAGTTTTTGATACAAATAAGTAATAATAAGAAATAA
TCAACCAATCTTTTCAAACTTTTAGCAACAAACAAGCAACATTTAAGGGT
TTATTTCTCTATGTAAGCTCCACCACTTCACATATTTTCGCCATCATGAC
ACCAAGACCAAAAATGTCCTTTACATAGAAGGTTTTGGGTCGGGTGTCAA
CAAATCTGTGACATGCTACTGCGAATCACTGGTGCAATATAAATCATTTT
TCAGTAGTTGAAATAGGTGAAAATGACTAGGGGCGAAGTCGGACTGTATG
GTTGGCAAAGGCACCAGTGACAATGCGCATTATTCAATCGATGCTGTTGT
CAGATGGCTCGTGTAGCTTGGTGATTGTCAAGGAATAATCAGCGTTCAAT
GTAAATGTCAATTATTTTATAATTGATTATTCTATATTGATTATAAAGTG
TTTTGGTCACATTTTAATTGATCTATTTTTTACACATTATATGATAGAAT
AGTGAATAAACAAATCTAAAATTTCATATTTTACAAAAATTTATAATGAC
CAGCTATGATTCATAGTTATTTGACATTATTATTATTATTATTTA
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protein sequence of SMED30034542-orf-1

>SMED30034542-orf-1 ID=SMED30034542-orf-1|Name=SMED30034542-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=149bp
MCKFKERASSSVKCDIQSHVKAFAVKLGILIKDVKKENFCHLPITQNLLA
KNHWFHFQTKHYWIHWKSCKEFQLRFKELRFHEQDIQVFRNPFSIDIENV
DTIYQMELSELQNCDSLKDAFSLSSLPNFYASSSPRHIPISGTMHSKW*
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