G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30034539
Length (bp)1245
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30034539) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30034539) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30034539) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30034539) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30034539

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
muscle cellSMED30034539SMESG000006006.1 dd_Smed_v4_13150_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30034539SMESG000006006.1 Contig54618newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30034539SMESG000006006.1 Contig54618uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30034539SMESG000006006.1 dd_Smed_v4_13150_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Match: CNMaR (gene:FBgn0053696 transcript:FBtr0091684)

HSP 1 Score: 52.7582 bits (125), Expect = 3.429e-7
Identity = 48/216 (22.22%), Postives = 99/216 (45.83%), Query Frame = 1 
Query:  190 YLIPFIAIFGIVGNVLVILVFLRKRISRFNVYXXXXXXXXXXXXXXXSFLDDFLGRGLYYATSGKSYVKFDTISKSS-CIAMEYFPNVMYFSSGFVMVIFTFDRFFVLMWPFKFKGTGGVRKSIIACVAVYSLGFITGIP-LGISHQLKASNDKWGIEGKICQISSNDSHLATLSLYYESVIVYVIPTVLIFIVNTFIIYEIIKMTKIRSTIILDK 831
            Y IP +   G +GN+L + VF R ++ + +    +     +LA+    FL     + L       +++  D  +++  C    +F  +  F S + +V FT +RF  +++P K +    VR++ I    +  +G +  +P + I+  +        +   IC ++S       L  Y+++++VY +P   I ++NT     + K   +R T+ + K
Sbjct:   64 YYIPVLCCTGSIGNILSVFVFFRTKLRKLS----SSFYLAALAVSDTCFLAGLFAQWL-------NFLNVDIYNQNYFCQFFTFFSYLASFCSVWFVVAFTVERFIAVIYPLKRQTMCTVRRAKIVLFCLTLVGCLHCLPYIVIAKPVFMPK----LNTTICDLNSEYKEQLALFNYWDTIVVYAVPFTTIAVLNTCTGCTVWKFATVRRTLTMHK 264
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183QJJ7 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 5.084e-52
Identity = 114/283 (40.28%), Postives = 175/283 (61.84%), Query Frame = 1 
Query:  181 YRAYLIPFIAIFGIVGNVLVILVFLRKR-ISRFNVYXXXXXXXXXXXXXXXSFLDDFLGRGLYYATSGKSYVKFDTISKSSCIAMEYFPNVMYFSSGFVMVIFTFDRFFVLMWPFKFKGTGGVRKSIIACVAVYSLGFITGIPLGISHQLKASNDKWGIEGKICQISSNDSHLATLSLYYESVIVYVIPTVLIFIVNTFIIYEIIKMTKIRSTII-LDKQANHLEMCRIVGMLAVSSTFLLFTIPLSVVIIIRL-----QIDH--PNGKNLRALSKLFSSLKD 1002
            +RAYL P IA+ G++GN  +IL+FL+++ +SRF++YSI L I N + L +N+F+DDFLGRGLYY T+ + Y K DTISK  C ++EY  N MYF+S +++VIF+ DR   +  P KF        ++IAC+ VYS+G I+  PL     L   ND        C++ S +  +A  ++ +E+++ + IP  L+  +N FI  E+ K+   R+T++  D   N +EM R+ G LA+S+ FLL  +P+   ++IRL      +D   P   N+  LS+LFSS+KD
Sbjct:   17 FRAYLCPIIAMIGVIGNSFIILIFLKEKPLSRFSIYSIFLAITNMITLIMNTFIDDFLGRGLYYLTNHQYYFKLDTISKFWCKSVEYLSNTMYFTSSYLIVIFSIDRLLTIHKPIKFYSIYHKHWALIACILVYSIGIISNSPLLFVQTL--INDTSSRTNFTCRMIS-EHPIAKFTIAFETIVTFTIPFCLVLFLNIFICIELWKLKIHRTTLLPTDCSRNRMEMGRVFGHLALSTAFLLLYLPMVCFVLIRLSQSLSHVDRHKPYAMNIIDLSRLFSSVKD 296
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183P8J0 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS10676 PE=4 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 1.977e-50
Identity = 115/298 (38.59%), Postives = 181/298 (60.74%), Query Frame = 1 
Query:  148 NTMSIYEFLNI---YRAYLIPFIAIFGIVGNVLVILVFLR-KRISRFNVYXXXXXXXXXXXXXXXSFLDDFLGRGLYYATSGKSYVKFDTISKSSCIAMEYFPNVMYFSSGFVMVIFTFDRFFVLMWPFKFKGTGGVRKSIIACVAVYSLGFITGIPLGISHQLKASNDKWGIEGKICQISSNDSHLATLSLYYESVIVYVIPTVLIFIVNTFIIYEIIKMTKIRSTIIL--DKQANHLEMCRIVGMLAVSSTFLLFTIPLSVVIIIRLQI-------DHPNGKNLRALSKLFSSLKD 1002
            N  +I ++ N+   +RAY+ P IAI G++GN  ++++FL+ K++SRF++YSI L IAN + L +N+F+DDFLGRGLYYAT+ + Y K DTIS   C ++EY  N MYF+S +++VIF+ DR   +  P KF        ++IAC+ VYS+G ++  PL     L    DK       C++ S +  +A  ++ +E+++ + IP  L+  +N FI  E+ K+ K+  T +L  D   N +EM R+ G LA+S+ FLL  +P+   ++IRL +         P    +  LS+LFSS+KD
Sbjct:    3 NNTTITDYSNLVGSFRAYICPIIAIIGVIGNSFIVIIFLKEKQLSRFSIYSIFLAIANIITLIMNTFIDDFLGRGLYYATNHQFYFKLDTISTFWCKSVEYISNTMYFTSSYLIVIFSIDRLLTIHKPIKFYSIYHKHWALIACILVYSMGILSNSPLLFVQTLMV--DKSSRTNFTCRMIS-EHPVAKFTITFETIVTFTIPFCLVLFLNVFICIELWKL-KVNRTALLPADSSRNRMEMGRVFGHLALSTAFLLLYLPMVCFVLIRLSLTLSHVDRHKPYAMCIIDLSRLFSSVKD 296
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A430QMQ3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma bovis OX=6184 GN=DC041_0001525 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 9.074e-50
Identity = 114/298 (38.26%), Postives = 180/298 (60.40%), Query Frame = 1 
Query:  148 NTMSIYEFLNI---YRAYLIPFIAIFGIVGNVLVILVFLR-KRISRFNVYXXXXXXXXXXXXXXXSFLDDFLGRGLYYATSGKSYVKFDTISKSSCIAMEYFPNVMYFSSGFVMVIFTFDRFFVLMWPFKFKGTGGVRKSIIACVAVYSLGFITGIPLGISHQLKASNDKWGIEGKICQISSNDSHLATLSLYYESVIVYVIPTVLIFIVNTFIIYEIIKMTKIRSTIIL--DKQANHLEMCRIVGMLAVSSTFLLFTIPLSVVIIIRLQI-------DHPNGKNLRALSKLFSSLKD 1002
            N  +I ++ N+   +RAY+ P IAI G++GN  ++++FL+ K++SRF++YSI L IAN + L +N+F+DDFLGRGLYYAT+ + Y K DTIS   C ++EY  N MYF+S +++VIF+ DR   +  P KF        ++IAC+ VYS+G ++  PL     L    D        C++ S +  +A  ++ +E+++ + IP  L+  +N FI  E+ K+ K+  T +L  D   N +EM R+ G LA+S+ FLL  +P+   ++IRL +         P    +  LS+LFSS+KD
Sbjct:    3 NNTTITDYSNLVGSFRAYICPIIAIIGVIGNSFIVIIFLKEKQLSRFSIYSIFLAIANIITLIMNTFIDDFLGRGLYYATNHQFYFKLDTISTFWCKSVEYISNTMYFTSSYLIVIFSIDRLLTIHKPIKFYSIYHKHWALIACILVYSMGILSNSPLLFVQTLMV--DTSSRTNFTCRMIS-EHPVAKFTITFETIVTFTIPFCLVLFLNVFICIELWKL-KVNRTALLPADSSRNRMEMGRVFGHLALSTAFLLLYLPMVCFVLIRLSLTLSHVDRHKPYAMCIIDLSRLFSSVKD 296
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183KKN2 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS15594 PE=4 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 9.980e-50
Identity = 114/299 (38.13%), Postives = 180/299 (60.20%), Query Frame = 1 
Query:  145 KNTMSIYEFLNI---YRAYLIPFIAIFGIVGNVLVILVFLR-KRISRFNVYXXXXXXXXXXXXXXXSFLDDFLGRGLYYATSGKSYVKFDTISKSSCIAMEYFPNVMYFSSGFVMVIFTFDRFFVLMWPFKFKGTGGVRKSIIACVAVYSLGFITGIPLGISHQLKASNDKWGIEGKICQISSNDSHLATLSLYYESVIVYVIPTVLIFIVNTFIIYEIIKMTKIRSTIIL--DKQANHLEMCRIVGMLAVSSTFLLFTIPLSVVIIIRLQI-------DHPNGKNLRALSKLFSSLKD 1002
             N  +I ++ N+   +RAY+ P IAI G++GN  ++++FL+ K++SRF++YSI L IAN + L +N+F+DDFLGRGLYYAT+ + Y K DTIS   C ++EY  N MYF+S +++VIF+ DR   +  P KF        ++IAC+ VYS+G ++  PL     L    D        C++ S +  +A  ++ +E+++ + IP  L+  +N FI  E+ K+ K+  T +L  D   N +EM R+ G LA+S+ FLL  +P+   ++IRL +         P    +  LS+LFSS+KD
Sbjct:    2 SNNTTITDYSNLVGSFRAYICPIIAIIGVIGNSFIVIIFLKEKQLSRFSIYSIFLAIANIITLIMNTFIDDFLGRGLYYATNHQFYFKLDTISTFWCKSVEYISNTMYFTSSYLIVIFSIDRLLTIHKPIKFYSIYHKHWALIACILVYSMGILSNSPLLFVQTLMV--DTSSRTNFTCRMIS-EHPVAKFTITFETIVTFTIPFCLVLFLNVFICIELWKL-KVNRTALLPADSSRNRMEMGRVFGHLALSTAFLLLYLPMVCFVLIRLSLTLSHVDRHKPYAMCIIDLSRLFSSVKD 296
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183L9P8 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS523 PE=4 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 3.439e-49
Identity = 115/298 (38.59%), Postives = 180/298 (60.40%), Query Frame = 1 
Query:  148 NTMSIYEFLNI---YRAYLIPFIAIFGIVGNVLVILVFLR-KRISRFNVYXXXXXXXXXXXXXXXSFLDDFLGRGLYYATSGKSYVKFDTISKSSCIAMEYFPNVMYFSSGFVMVIFTFDRFFVLMWPFKFKGTGGVRKSIIACVAVYSLGFITGIPLGISHQLKASNDKWGIEGKICQISSNDSHLATLSLYYESVIVYVIPTVLIFIVNTFIIYEIIKMTKIRSTIIL--DKQANHLEMCRIVGMLAVSSTFLLFTIPLSVVIIIRLQI-------DHPNGKNLRALSKLFSSLKD 1002
            N  +I ++ N+   +RAY+ P IAI G++GN  +I++FL+ K++SRF++YSI L IAN + L +N+F+DDFLGRGLYYAT+ + Y K DTIS   C ++EY  N MYF+S +++VIF+ DR   +  P KF        ++IAC+ VY +G ++  PL     L    DK       C++ S +  +A  ++ +E+++ + IP  L+  +N FI  E+ K+ K+  T +L  D   N +EM R+ G LA+S+ FLL  +P+   ++IRL +         P    +  LS+LFSS+KD
Sbjct:    3 NNTTITDYSNLVGSFRAYICPIIAIIGVIGNSFIIIIFLKEKQLSRFSIYSIFLAIANIITLIMNTFIDDFLGRGLYYATNHQFYFKLDTISTFWCKSVEYISNTMYFTSSYLIVIFSIDRLLTIHKPIKFYSIYHKHWAMIACILVYLMGILSNSPLLFVQTLMV--DKSSRTNFTCRMIS-EHPVAKFTITFETIVTFTIPFCLVLFLNIFICIELWKL-KVNRTALLPADSSRNCMEMGRVSGHLALSTAFLLLYLPMVCFVLIRLSLTLSHVDRHKPYAMCIIDLSRLFSSVKD 296
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000006006.1 (SMESG000006006.1)

HSP 1 Score: 587.8 bits (1514), Expect = 0.000e+0
Identity = 333/334 (99.70%), Postives = 333/334 (99.70%), Query Frame = 1 
Query:  154 MSIYEFLNIYRAYLIPFIAIFGIVGNVLVILVFLRKRISRFNVYXXXXXXXXXXXXXXXSFLDDFLGRGLYYATSGKSYVKFDTISKSSCIAMEYFPNVMYFSSGFVMVIFTFDRFFVLMWPFKFKGTGGVRKSIIACVAVYSLGFITGIPLGISHQLKASNDKWGIEGKICQISSNDSHLATLSLYYESVIVYVIPTVLIFIVNTFIIYEIIKMTKIRSTIILDKQANHLEMCRIVGMLAVSSTFLLFTIPLSVVIIIRLQIDHPNGKNLRALSKLFSSLKDXXXXXXXXXXXXXXXXXXXDLKMLFSSKVLYTFLTSSKSPNEEIEFNEIQK 1155
            MSIYEFLNIYRAYLIPFIAIFGIVGNVLVILVFLRKRISRFNVYSIALLIANSLALFINSFLDDFLGRGLYYATSGKSYVKFDTISKSSCIAMEYFPNVMYFSSGFVMVIFTFDRFFVLMWPFKFKGTGGVRKSIIACVAVY LGFITGIPLGISHQLKASNDKWGIEGKICQISSNDSHLATLSLYYESVIVYVIPTVLIFIVNTFIIYEIIKMTKIRSTIILDKQANHLEMCRIVGMLAVSSTFLLFTIPLSVVIIIRLQIDHPNGKNLRALSKLFSSLKDINYSINFFIYFIFLSYFKYDLKMLFSSKVLYTFLTSSKSPNEEIEFNEIQK
Sbjct:    1 MSIYEFLNIYRAYLIPFIAIFGIVGNVLVILVFLRKRISRFNVYSIALLIANSLALFINSFLDDFLGRGLYYATSGKSYVKFDTISKSSCIAMEYFPNVMYFSSGFVMVIFTFDRFFVLMWPFKFKGTGGVRKSIIACVAVYFLGFITGIPLGISHQLKASNDKWGIEGKICQISSNDSHLATLSLYYESVIVYVIPTVLIFIVNTFIIYEIIKMTKIRSTIILDKQANHLEMCRIVGMLAVSSTFLLFTIPLSVVIIIRLQIDHPNGKNLRALSKLFSSLKDINYSINFFIYFIFLSYFKYDLKMLFSSKVLYTFLTSSKSPNEEIEFNEIQK 334
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000072348.1 (SMESG000072348.1)

HSP 1 Score: 178.718 bits (452), Expect = 2.765e-52
Identity = 117/287 (40.77%), Postives = 167/287 (58.19%), Query Frame = 1 
Query:  175 NIYRAYLIPFIAIFGIVGNVLVILVFLRKRISRFNVYXXXXXXXXXXXXXXXSFLDDFLGRGLYYATSGKSYVKFDTISKSSCIAMEYFPNVMYFSSGFVMVIFTFDRFFVLMWPFKFKGTGGVRKSIIACVAVYSLGFITGIPLGISHQLKASNDKWGIEGKICQISSNDSHLATLSLYYESVIVYVIPTVLIFIVNTFIIYEIIKMTKIRSTIILDKQANH---LEMCRIVGMLAVSSTFLLFTIPLSVVIIIRLQI--------DHPNGKNLRALSKLFSSLKD 1002
            ++YR Y  P I+I GI+GN+ +I  F     ++F +Y  AL + NS AL  N+FLDDFLGRGL + T  K  +K + + + SC+ MEY PNVMYF+S +VMV F+ DR  VL  P K+    G R +I  C+AV  +G I   PL  S+ L+ +N         CQ+   +S + T  LY +S++++VIPTV +   N  +IY ++  TK R   +  ++ N     EM RIV  LA+S+ FLL TIPLS+ II+RL I         +    N+  L+KL SS+KD
Sbjct:   15 DVYRGYFCPVISILGILGNIFIIFTFAYNFNNKFVIYCCALALYNSFALLTNTFLDDFLGRGLGFLTKNKFSIKIEELCQGSCLFMEYVPNVMYFASSYVMVAFSLDRMLVLALPIKYSPYRGYRYTIFICLAVGVMGAIVAGPLLFSYSLEKTNANS--TEMSCQMI--NSKMVTFGLYLKSLVIFVIPTVFVASFN-IVIYCLLLQTKKRRQNLSSRKTNRGSSSEMGRIVAHLALSTVFLLLTIPLSITIILRLIIKKNSLYNYSYSYKNNVIHLTKLLSSIKD 296
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000016714.1 (SMESG000016714.1)

HSP 1 Score: 177.563 bits (449), Expect = 7.943e-52
Identity = 116/282 (41.13%), Postives = 172/282 (60.99%), Query Frame = 1 
Query:  175 NIYRAYLIPFIAIFGIVGNVLVILVFLRKRISRFNVYXXXXXXXXXXXXXXXSFLDDFLGRGLYYATSGKSYVKFDTISKSSCIAMEYFPNVMYFSSGFVMVIFTFDRFFVLMWPFKFKGTGGVRKSIIACVAVYSLGFITGIPLGISHQLKASNDKWGIEGKICQISSNDSHLATLSLYYESVIVYVIPTVLIFIVNTFIIYEIIKMTKI-RSTIILDKQANHLEMCRIVGMLAVSSTFLLFTIPLSVVIIIRLQIDHPNGKN-----LRALSKLFSSLKD 1002
            NIYRA++ P  A+ GI GN +++ +F+R +I RF  Y + L I+N  A+  N+ LDDFLGRG+ Y TSG    K +  S +SC  +E+FP +MYF S +V+V+FT DR  V+ +PFKF     + ++I     +  L  I   PL  SH L  + +        C++S   SHL+ +SLY +S++++V PT++I ++N  IIY++ +M K  R T +L    +  EM RI G +A+S  FL+ TIPLS+VII+RL ++     N     L  LSKLFSS+KD
Sbjct:   12 NIYRAFMAPVAAVIGIFGNSIILFLFIRMKIRRFEYYCVILAISNLFAVIFNTLLDDFLGRGMEYLTSGAWSFKPNLWSNNSCRLLEFFPTMMYFCSAYVLVVFTLDRCLVMSYPFKFVPNSHLTQTIFVSFILLLLSCIVSFPLTFSHHLFITKNI----SNSCEVS--KSHLSVVSLYIKSLLIFVSPTLIIIVLNVVIIYQLTQMQKYQRITTMLP--VSRPEMNRIFGHMALSIAFLILTIPLSLVIILRLSLNKSEINNDPNSILTQLSKLFSSIKD 285
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000003388.1 (SMESG000003388.1)

HSP 1 Score: 159.073 bits (401), Expect = 1.893e-44
Identity = 113/308 (36.69%), Postives = 171/308 (55.52%), Query Frame = 1 
Query:  181 YRAYLIPFIAIFGIVGNVLVILVF-LRKRISRFNVYXXXXXXXXXXXXXXXSFLDDFLGRGL-----YYATSGKSYVKFDTISKSSCIAMEYFPNVMYFSSGFVMVIFTFDRFFVLMWPFKFKGTGGVRKSIIACVAVYSLGFITGIPLGISHQLKASNDKWGIEGKICQISSNDSH--LATLSLYYESVIVYVIPTVLIFIVNTFIIYEI-------IKMTK-----------IRSTIILDKQANHLEMCRIVGMLAVSSTFLLFTIPLSVVIIIRLQIDHPNG--------KNLRALSKLFSSLKD 1002
            +RAY+ P +A+ GI GN+ VIL+F    ++SRF+VY I+L     +AL +NSF+DDFLGRGL     YY  +     K+DT S  SC  MEY PN M+F + FV V+F+ DR   +  P KF     ++ SI+ACV  +  GF   IPL +S+ L    +++GI  K C + +  +   +A   LY   +  + IPT ++ I+NT I  ++        +MTK           I  +++ +++    E+ R++G LAV+  FLL T+PL +VII+R+  D             + + LS+LFSSLKD
Sbjct:   25 FRAYVTPVVAVVGIFGNIFVILIFYFETKLSRFSVYCISLAFVQLVALIVNSFMDDFLGRGLSHVLGYYLLA----FKWDTHSDMSCKMMEYVPNTMFFIASFVTVLFSIDRLLTIHQPLKFHSVYYIKYSIMACVGTFLFGFAINIPLLLSYGLIKQENEFGITLK-CTVVATGARKFVADFGLYLVLLGSFTIPTFIVLILNTLISIKLWQLHLNRAEMTKSFDTSDSKSSAINPSLMKNQEK---ELNRVLGHLAVNVVFLLLTMPLVIVIILRMYSDDNKMYLTNLYLYNSFQHLSRLFSSLKD 324
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000040061.1 (SMESG000040061.1)

HSP 1 Score: 155.221 bits (391), Expect = 2.174e-43
Identity = 117/315 (37.14%), Postives = 185/315 (58.73%), Query Frame = 1 
Query:  175 NIYRAYLIPFIAIFGIVGNVLVILVFLRKRISRFNVYXXXXXXXXXXXXXXXSFLDDFLGRGLYYATSGKSYVKFDTISKSSCIAMEYFPNVMYFSSGFVMVIFTFDRFFVLMWPFKFKG-TGGVRKSIIACVAVYSLGFITGIPLGISHQLKA-SNDKWGIEGKICQISSNDSHLATLSLYYESVIVYVIPTVLIFIVNTFIIYEI----IKMTKIRSTIILDKQANHLEMCRIVGMLAVSSTFLLFTIPLSVVIIIRLQIDHP-------NGKNLRALSKLFSSLKDXXXXXXXXXXXXXXXXXXXDLKMLFS 1080
            +IYRA +IPF+ +FG+ GNV++I+V  R+ + RF VY   L +  S+AL  NSFLDDF GRGL + +SG+  +K D      C  ME+ P VMYF+S +VMV+   DR+FV+    +F   +  V+++   CV V  +G I   PL   + +   S +      K C++   DS  + + LY++S++V+V+P +++ I N FI+ ++    I   ++ S   + K  N   M +++G LA+SS FLL ++PL++ II+R  I  P        G  L   SK  SS+KD++Y+INF++   FL  F+ D+K LF+
Sbjct:   11 DIYRAIIIPFVVLFGVFGNVVIIVVLSRQFVGRFEVYCFTLAVVQSVALIFNSFLDDFFGRGLDFLSSGRINIKIDQSVNFMCKLMEFIPRVMYFASAYVMVVMAVDRYFVISKGSQFPAPSRQVKRAKFICVLVLFIGMILCSPLLWYYNINNLSYENSHFSNKSCKLY--DSVFSEILLYFQSLLVFVLPIIIVIICNFFIVNKVRQRRILRKRLSSASSIKKDPNSGSM-KLIGYLAISSGFLLISMPLAINIIVRSIITGPFKASKSDVGVTLSEASKFTSSIKDLHYAINFYLNVAFLEQFRKDVKSLFT 322
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CNMaR3.429e-722.22gene:FBgn0053696 transcript:FBtr0091684[more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183QJJ75.084e-5240.28G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A183P8J01.977e-5038.59G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A430QMQ39.074e-5038.26G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A183KKN29.980e-5038.13G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A183L9P83.439e-4938.59G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000006006.10.000e+099.70SMESG000006006.1[more]
SMESG000072348.12.765e-5240.77SMESG000072348.1[more]
SMESG000016714.17.943e-5241.13SMESG000016714.1[more]
SMESG000003388.11.893e-4436.69SMESG000003388.1[more]
SMESG000040061.12.174e-4337.14SMESG000040061.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034539 ID=SMED30034539|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1245bp
ATGATTTTCAACATAGAATTATCAATTGAAAATTGTGCTACATCAATGAA
ACATTTTTTATTGATTTGCTAGAATAAATTTTTAGTGTGCAGCACAAGCA
CAGTAATAGATGCCAATGCACAGTGCAATTGTATAAAGTTCTTCAAAAAT
ACAATGTCGATATATGAGTTTCTAAATATCTATCGTGCCTACCTGATTCC
ATTTATAGCGATTTTTGGAATTGTGGGAAATGTTTTGGTAATTCTTGTAT
TCTTGAGAAAAAGAATTTCCAGATTCAATGTCTATTCGATTGCTTTACTT
ATTGCAAATTCTTTGGCACTCTTTATCAATTCTTTTCTTGATGACTTCCT
AGGACGAGGCTTGTATTATGCAACCTCTGGTAAGTCGTACGTTAAATTTG
ATACTATTTCTAAATCATCGTGTATCGCAATGGAATATTTTCCAAATGTT
ATGTATTTCAGCAGTGGTTTTGTGATGGTGATTTTTACATTTGATCGATT
TTTTGTGTTGATGTGGCCATTTAAATTCAAAGGCACCGGAGGAGTTAGAA
AATCAATAATTGCATGTGTAGCTGTCTACTCTCTTGGGTTTATTACTGGA
ATACCATTGGGAATAAGTCATCAATTGAAAGCCTCCAACGATAAGTGGGG
CATTGAGGGAAAGATTTGCCAAATTTCCTCAAATGACTCTCATCTAGCGA
CATTATCTTTGTATTACGAATCTGTCATTGTATATGTCATACCAACTGTC
CTAATTTTCATTGTCAATACTTTTATCATCTATGAAATCATAAAAATGAC
CAAAATAAGGTCCACGATAATCCTGGATAAACAAGCCAATCACTTGGAAA
TGTGTCGGATAGTTGGAATGTTGGCGGTCTCGTCGACTTTTTTACTTTTT
ACAATACCACTGTCTGTAGTAATTATTATCAGGCTTCAGATTGATCATCC
CAATGGGAAAAATCTAAGAGCATTATCAAAATTGTTTTCGTCTTTAAAAG
ACATCAATTACAGTATCAACTTTTTTATTTATTTTATATTTTTATCATAT
TTTAAATACGATTTGAAAATGTTGTTTTCGTCGAAAGTATTGTATACTTT
TCTCACTTCATCCAAATCTCCCAACGAGGAAATTGAATTTAATGAGATTC
AGAAGTAGCTATGTGTACTTAAATTTAAATATATTATTTTTTTGTTTAAA
TATCAAGTAACTATTTACAAAAAACATTTGATATTTTTGTATCAG
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protein sequence of SMED30034539-orf-1

>SMED30034539-orf-1 ID=SMED30034539-orf-1|Name=SMED30034539-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=335bp
MSIYEFLNIYRAYLIPFIAIFGIVGNVLVILVFLRKRISRFNVYSIALLI
ANSLALFINSFLDDFLGRGLYYATSGKSYVKFDTISKSSCIAMEYFPNVM
YFSSGFVMVIFTFDRFFVLMWPFKFKGTGGVRKSIIACVAVYSLGFITGI
PLGISHQLKASNDKWGIEGKICQISSNDSHLATLSLYYESVIVYVIPTVL
IFIVNTFIIYEIIKMTKIRSTIILDKQANHLEMCRIVGMLAVSSTFLLFT
IPLSVVIIIRLQIDHPNGKNLRALSKLFSSLKDINYSINFFIYFIFLSYF
KYDLKMLFSSKVLYTFLTSSKSPNEEIEFNEIQK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
Vocabulary: INTERPRO
TermDefinition
IPR001024PLAT/LH2_dom
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0004930G-protein coupled receptor activity
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 10..34
score: 27.65
coord: 97..119
score: 18.67
coord: 275..301
score: 22.08
coord: 41..62
score: 19.69
coord: 241..264
score: 20.33
IPR000276G protein-coupled receptor, rhodopsin-likeSMARTSM013817TM_GPCR_Srsx_2coord: 19..308
e-value: 0.0065
score: -66.6
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 25..289
e-value: 3.2E-14
score: 52.9
IPR000276G protein-coupled receptor, rhodopsin-likePROSITEPS00237G_PROTEIN_RECEP_F1_1coord: 103..119
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 4..314
e-value: 2.1E-36
score: 127.4
NoneNo IPR availablePANTHERPTHR24243:SF214G-PROTEIN COUPLED RECEPTOR 19-RELATEDcoord: 10..309
NoneNo IPR availablePANTHERPTHR24243G-PROTEIN COUPLED RECEPTORcoord: 10..309
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 9..300
e-value: 1.14501E-21
score: 91.1552
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 3..315
NoneNo IPR availableTMHMMTMhelixcoord: 41..63
NoneNo IPR availableTMHMMTMhelixcoord: 134..156
NoneNo IPR availableTMHMMTMhelixcoord: 15..34
NoneNo IPR availableTMHMMTMhelixcoord: 191..213
NoneNo IPR availableTMHMMTMhelixcoord: 278..300
NoneNo IPR availableTMHMMTMhelixcoord: 91..113
NoneNo IPR availableTMHMMTMhelixcoord: 236..258
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 25..293
score: 21.756