Transmembrane protein 220

Overview
NameTransmembrane protein 220
Smed IDSMED30034451
Length (bp)731
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Transmembrane protein 220 (SMED30034451) t-SNE clustered cells

Violin plots show distribution of expression levels for Transmembrane protein 220 (SMED30034451) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Transmembrane protein 220 (SMED30034451) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Transmembrane protein 220 (SMED30034451) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30034451

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30034451h1SMcG0012974 dd_Smed_v4_1981_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30034451h1SMcG0012974 dd_Smed_v4_1981_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30034451h1SMcG0012974 dd_Smed_v4_1981_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30034451h1SMcG0012974 dd_Smed_v4_1981_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30034451h1SMcG0012974 dd_Smed_v4_1981_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Transmembrane protein 220 vs. Ensembl Human
Match: TMEM220 (transmembrane protein 220 [Source:HGNC Symbol;Acc:HGNC:33757])

HSP 1 Score: 62.3882 bits (150), Expect = 1.221e-11
Identity = 39/111 (35.14%), Postives = 67/111 (60.36%), Query Frame = 2 
Query:  128 IWRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKL--NFLSLEEGREVSGLLIVSAWCVL 454
            +WR  N +M  FF +AA+VQ NDPD  +W+ +YTIPA+   L  +  ++  N ++    ++++     +A+ +      ++LL +   N L  EEGRE+SGL+I++AW +L
Sbjct:    5 LWRACNGLMAAFFALAALVQVNDPDAEVWVVVYTIPAVLTLLVGLNPEVTGNVIWKSISAIHILFCTVWAVGL-----ASYLLHRTQQNILHEEEGRELSGLVIITAWIIL 110
[Close]
BLAST of Transmembrane protein 220 vs. Ensembl Xenopus
Match: tmem220 (transmembrane protein 220 [Source:NCBI gene;Acc:100135071])

HSP 1 Score: 55.8398 bits (133), Expect = 4.039e-9
Identity = 39/110 (35.45%), Postives = 59/110 (53.64%), Query Frame = 2 
Query:  125 NIWRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAWCVL 454
             +WR  N +M  FF +AA VQ NDPD  +WI +Y IPA      ++   +  + M+     ++  A    A++  +S    F   K + L  EEGRE+SGLLI++ W +L
Sbjct:   29 RLWRTCNFVMFGFFSLAAYVQINDPDAEMWIAVYMIPATLILFVSIKPDVTGHVMWKSLADLHAAACAFGAIY--LSRCLYFHTTK-SILHEEEGRELSGLLIIAGWLLL 135
[Close]
BLAST of Transmembrane protein 220 vs. Ensembl Mouse
Match: Tmem220 (transmembrane protein 220 [Source:MGI Symbol;Acc:MGI:2443691])

HSP 1 Score: 57.7658 bits (138), Expect = 5.040e-10
Identity = 44/114 (38.60%), Postives = 63/114 (55.26%), Query Frame = 2 
Query:  125 NIWRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVI---SNAQAFLLK-KLNFLSLEEGREVSGLLIVSAWCVL 454
             +WR  N +M  FF +AAVVQ NDPD  +W+ +Y IPA+   L      +  N ++    ++++       LF  +     A  FLL  K N L+ EEGRE+SGL+IV+AW  L
Sbjct:   11 GLWRACNALMAAFFALAAVVQVNDPDAELWVVVYMIPAVLTLLVGFNPLVTGNFIWKSVSAIHM-------LFCALWAGGLAYHFLLHAKQNLLNEEEGRELSGLVIVTAWMAL 117
[Close]
BLAST of Transmembrane protein 220 vs. UniProt/SwissProt
Match: sp|Q6QAJ8|TM220_HUMAN (Transmembrane protein 220 OS=Homo sapiens OX=9606 GN=TMEM220 PE=1 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 5.864e-11
Identity = 39/111 (35.14%), Postives = 67/111 (60.36%), Query Frame = 2 
Query:  128 IWRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKL--NFLSLEEGREVSGLLIVSAWCVL 454
            +WR  N +M  FF +AA+VQ NDPD  +W+ +YTIPA+   L  +  ++  N ++    ++++     +A+ +      ++LL +   N L  EEGRE+SGL+I++AW +L
Sbjct:    5 LWRACNGLMAAFFALAALVQVNDPDAEVWVVVYTIPAVLTLLVGLNPEVTGNVIWKSISAIHILFCTVWAVGL-----ASYLLHRTQQNILHEEEGRELSGLVIITAWIIL 110
[Close]
BLAST of Transmembrane protein 220 vs. UniProt/SwissProt
Match: sp|Q4V7Q8|TM220_XENLA (Transmembrane protein 220 OS=Xenopus laevis OX=8355 GN=tmem220 PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 2.135e-9
Identity = 42/110 (38.18%), Postives = 60/110 (54.55%), Query Frame = 2 
Query:  125 NIWRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAWCVL 454
             +WR  N IM  FF +AA VQ NDPD  +WI IY IPA+     ++   I  + ++ +   ++       A++  +S    F   K N L  EEGRE+SGLLI+S W +L
Sbjct:   29 RLWRICNFIMFGFFSLAAYVQINDPDAEMWIVIYMIPAVLILFVSIKPDITGHVIWKLLADLHSAVCAVGAIY--LSGCLYFYTSK-NILHEEEGRELSGLLIISGWLLL 135
[Close]
BLAST of Transmembrane protein 220 vs. UniProt/SwissProt
Match: sp|Q8BP07|TM220_MOUSE (Transmembrane protein 220 OS=Mus musculus OX=10090 GN=Tmem220 PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 3.525e-9
Identity = 44/114 (38.60%), Postives = 63/114 (55.26%), Query Frame = 2 
Query:  125 NIWRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVI---SNAQAFLLK-KLNFLSLEEGREVSGLLIVSAWCVL 454
             +WR  N +M  FF +AAVVQ NDPD  +W+ +Y IPA+   L      +  N ++    ++++       LF  +     A  FLL  K N L+ EEGRE+SGL+IV+AW  L
Sbjct:   11 GLWRACNALMAAFFALAAVVQVNDPDAELWVVVYMIPAVLTLLVGFNPLVTGNFIWKSVSAIHM-------LFCALWAGGLAYHFLLHAKQNLLNEEEGRELSGLVIVTAWMAL 117
[Close]
BLAST of Transmembrane protein 220 vs. TrEMBL
Match: A0A2R2MKU5 (transmembrane protein 220 isoform X2 OS=Lingula unguis OX=7574 GN=LOC106178422 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.054e-12
Identity = 40/126 (31.75%), Postives = 74/126 (58.73%), Query Frame = 2 
Query:   68 QKSEINYTSIDNSEQSKCCNIWRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAW 445
            +  ++  +S++ +  +     W+ +N++  VFF +AA VQ NDPDPYIWIPIY IP +     A+   ++ N+ +     +++   ++  L++ ++  +  L  ++N L  EEGRE+ GL++V AW
Sbjct:    3 KPQDVEESSMEGTGSAGWQKAWQAINVVAAVFFALAAYVQINDPDPYIWIPIYFIPTLLCLTIAIKPILVENTAWRNVCVIHMAICLTGTLYLTVTVLEMTLSTRVNPLQHEEGRELGGLILVMAW 128
[Close]
BLAST of Transmembrane protein 220 vs. TrEMBL
Match: A0A1S3K318 (transmembrane protein 220 isoform X1 OS=Lingula unguis OX=7574 GN=LOC106178422 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.133e-12
Identity = 40/126 (31.75%), Postives = 74/126 (58.73%), Query Frame = 2 
Query:   68 QKSEINYTSIDNSEQSKCCNIWRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAW 445
            +  ++  +S++ +  +     W+ +N++  VFF +AA VQ NDPDPYIWIPIY IP +     A+   ++ N+ +     +++   ++  L++ ++  +  L  ++N L  EEGRE+ GL++V AW
Sbjct:    3 KPQDVEESSMEGTGSAGWQKAWQAINVVAAVFFALAAYVQINDPDPYIWIPIYFIPTLLCLTIAIKPILVENTAWRNVCVIHMAICLTGTLYLTVTVLEMTLSTRVNPLQHEEGRELGGLILVMAW 128
[Close]
BLAST of Transmembrane protein 220 vs. TrEMBL
Match: R7THW5 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_217562 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.237e-9
Identity = 38/121 (31.40%), Postives = 69/121 (57.02%), Query Frame = 2 
Query:   68 QKSEINYTSIDNSEQSKCCNIWRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLL 430
            +  +   +S + +  +K  NIWR +N +M +FF ++A VQ NDPDPYIWIP+Y +PA+ +    V+  +  + ++     +++   ++ AL+++    +    K +N L  EEGR V  L+
Sbjct:   13 EDEQGGKSSPEENGSNKWRNIWRSINAVMTLFFSLSAYVQLNDPDPYIWIPVYAVPAVLSLSMCVHPTVPSHPVWRTVCVLHIALCVAGALYLLAVMIEILTGKIVNPLLHEEGRRVPFLI 133
[Close]
BLAST of Transmembrane protein 220 vs. TrEMBL
Match: T1ISY2 (Uncharacterized protein OS=Strigamia maritima OX=126957 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.808e-9
Identity = 49/144 (34.03%), Postives = 81/144 (56.25%), Query Frame = 2 
Query:  128 IWRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVI---SYALFIVISN--AQAFLLKK---------LNFLSLEEGREVSGLLIVSAWCVLQLWYVVNISEKSLQ--RYFIS 511
            +W+F+NI+M +FF++A +VQ+ND D  +WIP+Y IPA  +    +  KI  +SM++    V+    I   +Y + +++ N   ++FLL++          N L  EEGRE+ GL++V  W  L++   V     +LQ  RY I 
Sbjct:    8 VWKFVNILMFLFFLLATLVQFNDDDACVWIPVYVIPAALSLAIVIKPKITSDSMWLTVTHVHTACCICIFAYIVALLLQNMHKESFLLERKLNAKQQVHWNLLYYEEGRELVGLILVLIW--LKISKTVMTPGSTLQKSRYLIG 149
[Close]
BLAST of Transmembrane protein 220 vs. TrEMBL
Match: A0A2J8MGW3 (TMEM220 isoform 4 OS=Pan troglodytes OX=9598 GN=CK820_G0021164 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 5.145e-9
Identity = 39/111 (35.14%), Postives = 66/111 (59.46%), Query Frame = 2 
Query:  128 IWRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKL--NFLSLEEGREVSGLLIVSAWCVL 454
            +WR  N +M  FF +AA VQ NDPD  +W+ +YTIPA+   L  +  ++  N ++    ++++     +A+ +      ++LL +   N L  EEGRE+SGL+I++AW +L
Sbjct:    5 LWRACNGLMAAFFALAAFVQVNDPDAEVWVVVYTIPAVLTLLVGLNPEVTGNVIWKSISAIHILFCTVWAVGLA-----SYLLHRTQQNILHEEEGRELSGLVIITAWIIL 110
[Close]
BLAST of Transmembrane protein 220 vs. Ensembl Nematostella
Match: EDO47950 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RKR3])

HSP 1 Score: 61.2326 bits (147), Expect = 7.204e-12
Identity = 46/126 (36.51%), Postives = 73/126 (57.94%), Query Frame = 2 
Query:  131 WRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAWCVLQLWYVVNI-SEKSLQRYF 505
            WR  N++M VFF +AA VQ NDPDP IWI +Y IP + + + A++ ++    ++      ++ A I  AL++    +       ++ L  EEGRE+SGLL V  W  + L  +VN+ S K++Q+Y 
Sbjct:   25 WRVANMLMAVFFALAAYVQINDPDPAIWITVYAIPCLLSSVVAIHFQVQGYFLWRALTVSHLCACICGALYLSTRVSVHVRNGMVHPLEHEEGRELSGLLFVIMW--MALLKLVNLKSAKTIQKYL 148
[Close]
BLAST of Transmembrane protein 220 vs. Planmine SMEST
Match: SMESG000010767.1 (SMESG000010767.1)

HSP 1 Score: 351.288 bits (900), Expect = 3.108e-124
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 2 
Query:   29 MKDFHMFSKRIQIQKSEINYTSIDNSEQSKCCNIWRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAWCVLQLWYVVNISEKSLQRYFISKKLIIMSVTAXXXXXXXXXXXXXCHISNIKEHVKYCHEMC 631
            MKDFHMFSKRIQIQKSEINYTSIDNSEQSKCCNIWRFMNIIMMVFFIMAAVVQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAWCVLQLWYVVNISEKSLQRYFISKKLIIMSVTALILSLIPPILWSICHISNIKEHVKYCHEMC
Sbjct:    1 MKDFHMFSKRIQIQKSEINYTSIDNSEQSKCCNIWRFMNIIMMVFFIMAAVVQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAWCVLQLWYVVNISEKSLQRYFISKKLIIMSVTALILSLIPPILWSICHISNIKEHVKYCHEMC 201
[Close]
BLAST of Transmembrane protein 220 vs. Planmine SMEST
Match: SMESG000010767.1 (SMESG000010767.1)

HSP 1 Score: 339.347 bits (869), Expect = 1.243e-119
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 2 
Query:   44 MFSKRIQIQKSEINYTSIDNSEQSKCCNIWRFMNXXXXXXXXXXXXXQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAWCVLQLWYVVNISEKSLQRYFISKKLIIMSVTAXXXXXXXXXXXXXCHISNIKEHVKYCHEMC 631
            MFSKRIQIQKSEINYTSIDNSEQSKCCNIWRFMNIIMMVFFIMAAVVQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAWCVLQLWYVVNISEKSLQRYFISKKLIIMSVTALILSLIPPILWSICHISNIKEHVKYCHEMC
Sbjct:    1 MFSKRIQIQKSEINYTSIDNSEQSKCCNIWRFMNIIMMVFFIMAAVVQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAWCVLQLWYVVNISEKSLQRYFISKKLIIMSVTALILSLIPPILWSICHISNIKEHVKYCHEMC 196
[Close]
BLAST of Transmembrane protein 220 vs. Planmine SMEST
Match: SMESG000010767.1 (SMESG000010767.1)

HSP 1 Score: 272.707 bits (696), Expect = 7.446e-94
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 2 
Query:  185 QYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAWCVLQLWYVVNISEKSLQRYFISKKLIIMSVTAXXXXXXXXXXXXXCHISNIKEHVKYCHEMC 631
            QYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAWCVLQLWYVVNISEKSLQRYFISKKLIIMSVTALILSLIPPILWSICHISNIKEHVKYCHEMC
Sbjct:   12 QYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVISYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAWCVLQLWYVVNISEKSLQRYFISKKLIIMSVTALILSLIPPILWSICHISNIKEHVKYCHEMC 160
The following BLAST results are available for this feature:
BLAST of Transmembrane protein 220 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
TMEM2201.221e-1135.14transmembrane protein 220 [Source:HGNC Symbol;Acc:... [more]
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BLAST of Transmembrane protein 220 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transmembrane protein 220 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transmembrane protein 220 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transmembrane protein 220 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tmem2204.039e-935.45transmembrane protein 220 [Source:NCBI gene;Acc:10... [more]
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BLAST of Transmembrane protein 220 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Tmem2205.040e-1038.60transmembrane protein 220 [Source:MGI Symbol;Acc:M... [more]
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BLAST of Transmembrane protein 220 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q6QAJ8|TM220_HUMAN5.864e-1135.14Transmembrane protein 220 OS=Homo sapiens OX=9606 ... [more]
sp|Q4V7Q8|TM220_XENLA2.135e-938.18Transmembrane protein 220 OS=Xenopus laevis OX=835... [more]
sp|Q8BP07|TM220_MOUSE3.525e-938.60Transmembrane protein 220 OS=Mus musculus OX=10090... [more]
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BLAST of Transmembrane protein 220 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2R2MKU54.054e-1231.75transmembrane protein 220 isoform X2 OS=Lingula un... [more]
A0A1S3K3184.133e-1231.75transmembrane protein 220 isoform X1 OS=Lingula un... [more]
R7THW51.237e-931.40Uncharacterized protein OS=Capitella teleta OX=283... [more]
T1ISY21.808e-934.03Uncharacterized protein OS=Strigamia maritima OX=1... [more]
A0A2J8MGW35.145e-935.14TMEM220 isoform 4 OS=Pan troglodytes OX=9598 GN=CK... [more]
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BLAST of Transmembrane protein 220 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transmembrane protein 220 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transmembrane protein 220 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transmembrane protein 220 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO479507.204e-1236.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Transmembrane protein 220 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transmembrane protein 220 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000010767.13.108e-124100.00SMESG000010767.1[more]
SMESG000010767.11.243e-119100.00SMESG000010767.1[more]
SMESG000010767.17.446e-94100.00SMESG000010767.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034451 ID=SMED30034451|Name=Transmembrane protein 220|organism=Schmidtea mediterranea sexual|type=transcript|length=731bp
ATAATTAATTAATATTTCCCCGAATAAAATGAAAGACTTTCACATGTTTA
GTAAAAGAATTCAAATTCAAAAATCAGAAATTAATTATACTAGCATAGAT
AACTCGGAACAATCAAAATGTTGTAATATATGGCGTTTTATGAACATTAT
AATGATGGTGTTTTTTATTATGGCAGCTGTGGTGCAATACAACGATCCCG
ATCCTTATATATGGATACCAATTTACACGATTCCGGCAATATTTGCATTC
TTGAGCGCGGTATATGAGAAAATTATTAGAAATTCCATGTTCATGGTAAC
TGAGTCTGTTTATGTAGGCGCTGTGATTTCCTATGCTCTGTTTATTGTTA
TTAGTAATGCTCAGGCATTTCTATTGAAGAAACTAAATTTTTTGTCTTTG
GAAGAAGGTCGAGAAGTTTCAGGATTATTGATAGTTTCGGCATGGTGCGT
GTTGCAGTTGTGGTATGTTGTAAATATCTCAGAAAAGTCACTCCAAAGAT
ACTTTATCAGTAAAAAATTAATTATCATGTCAGTAACAGCCTTAATTCTC
TCATTAATCCCACCAATTCTGTGGAGTATTTGTCACATAAGCAACATAAA
AGAACATGTAAAATATTGTCATGAAATGTGTTGAAATTCACTTTAAAAAT
CAGTGATATTTATTACAAATTAGCACCTTATGTCGTTGATAAATTAAGTT
CAAATGTTTCATTTTTAATATACAATATTGG
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protein sequence of SMED30034451-orf-1

>SMED30034451-orf-1 ID=SMED30034451-orf-1|Name=SMED30034451-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=202bp
MKDFHMFSKRIQIQKSEINYTSIDNSEQSKCCNIWRFMNIIMMVFFIMAA
VVQYNDPDPYIWIPIYTIPAIFAFLSAVYEKIIRNSMFMVTESVYVGAVI
SYALFIVISNAQAFLLKKLNFLSLEEGREVSGLLIVSAWCVLQLWYVVNI
SEKSLQRYFISKKLIIMSVTALILSLIPPILWSICHISNIKEHVKYCHEM
C*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0002056medial muscle cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR029377TMEM220
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029377TMEM220 proteinPFAMPF15071TMEM220coord: 45..145
e-value: 1.1E-21
score: 77.5
NoneNo IPR availablePANTHERPTHR34262FAMILY NOT NAMEDcoord: 24..192
NoneNo IPR availableTMHMMTMhelixcoord: 60..79
NoneNo IPR availableTMHMMTMhelixcoord: 33..55
NoneNo IPR availableTMHMMTMhelixcoord: 164..186
NoneNo IPR availableTMHMMTMhelixcoord: 92..114
NoneNo IPR availableTMHMMTMhelixcoord: 129..151