Antigen KI-67

Overview


Name	Antigen KI-67
Smed ID	SMED30034422
Length (bp)	1566

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Antigen KI-67 (SMED30034422) t-SNE clustered cells

Violin plots show distribution of expression levels for Antigen KI-67 (SMED30034422) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Antigen KI-67 (SMED30034422) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Antigen KI-67 (SMED30034422) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30034422

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 38

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pigment cell	SMED30034422	h1SMcG0005241	SmedASXL_017551	SmedAsxl_ww_GCZZ01	PMID:29158443 He et al., 2017
pigment cell	SMED30034422	h1SMcG0005241	SmedASXL_012148	SmedAsxl_ww_GCZZ01	PMID:29158443 He et al., 2017
pigment cell	SMED30034422	h1SMcG0005241	SmedASXL_017550	SmedAsxl_ww_GCZZ01	PMID:29158443 He et al., 2017
X1 cell	SMED30034422	h1SMcG0005241	SmedASXL_017551	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X2 cell	SMED30034422	h1SMcG0005241	SmedASXL_017551	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X1 cell	SMED30034422	h1SMcG0005241	SmedASXL_012148	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X2 cell	SMED30034422	h1SMcG0005241	SmedASXL_012148	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X1 cell	SMED30034422	h1SMcG0005241	SmedASXL_017550	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X2 cell	SMED30034422	h1SMcG0005241	SmedASXL_017550	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
nervous system	SMED30034422	h1SMcG0005241	dd_Smed_v4_3777_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30034422	h1SMcG0005241	dd_Smed_v4_3777_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30034422	h1SMcG0005241	dd_Smed_v4_3777_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30034422	h1SMcG0005241	dd_Smed_v4_3777_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30034422	h1SMcG0005241	dd_Smed_v4_3777_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30034422	h1SMcG0005241	dd_Smed_v4_3777_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034422	h1SMcG0005241	Contig20539	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034422	h1SMcG0005241	Contig20539	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034422	h1SMcG0005241	Contig37647	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034422	h1SMcG0005241	Contig37647	newmark_ests	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30034422	h1SMcG0005241	dd_Smed_v4_13700_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30034422	h1SMcG0005241	dd_Smed_v4_13700_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30034422	h1SMcG0005241	dd_Smed_v4_2548_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30034422	h1SMcG0005241	dd_Smed_v4_2548_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30034422	h1SMcG0005241	dd_Smed_v4_2548_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30034422	h1SMcG0005241	dd_Smed_v4_2548_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parapharyngeal region	SMED30034422	h1SMcG0005241	dd_Smed_v6_13700_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
parapharyngeal region	SMED30034422	h1SMcG0005241	dd_Smed_v6_2548_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
parapharyngeal region	SMED30034422	h1SMcG0005241	dd_Smed_v6_3777_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
neoblast	SMED30034422	h1SMcG0005241	dd_Smed_v6_2548_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
neoblast	SMED30034422	h1SMcG0005241	dd_Smed_v6_3777_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
zeta neoblast	SMED30034422	h1SMcG0005241	dd_Smed_v4_3777_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
zeta neoblast	SMED30034422	h1SMcG0005241	dd_Smed_v4_13700_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
neoblast	SMED30034422	h1SMcG0005241	SMED30023035	smed_20140614	PMID:27523733 Lei et al., 2016
neoblast	SMED30034422	h1SMcG0005242	SMED30023035	smed_20140614	PMID:27523733 Lei et al., 2016
neoblast	SMED30034422	h1SMcG0005242	SMED_29717_V2_1	GPL14150_gene_models	PMID:22385657 Wagner et al., 2012
neoblast	SMED30034422	h1SMcG0005242	SMED_29717_V2_1	GPL14150	PMID:22385657 Wagner et al., 2012
neoblast	SMED30034422	h1SMcG0005241	SMED_29717_V2_1	GPL14150_gene_models	PMID:22385657 Wagner et al., 2012
neoblast	SMED30034422	h1SMcG0005241	SMED_29717_V2_1	GPL14150	PMID:22385657 Wagner et al., 2012

Note: Hover over icons to view figure legend

Homology

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BLAST of Antigen KI-67 vs. Ensembl Xenopus
Match: fbxo38 (F-box protein 38 [Source:Xenbase;Acc:XB-GENE-969087])

HSP 1 Score: 61.2326 bits (147), Expect = 3.954e-9
Identity = 27/100 (27.00%), Postives = 51/100 (51.00%), Query Frame = 3

Query:   57 GQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQS 356
            G++  + RNG +G        T   G    CDI I    +  +HC ++++ +++ F++++ S    T L  I +++P VL H  + T+     RF+ PQ+
Sbjct:    5 GEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINL-STVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQT 103

HSP 2 Score: 51.2174 bits (121), Expect = 5.131e-6
Identity = 36/106 (33.96%), Postives = 52/106 (49.06%), Query Frame = 3

Query:  633 KTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDSTKVRKRKFSIVPRGSSVGVINKDNDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVPAKR 950
            K VSFG +LSPE FD+ +PP  P+K+GATP            +R  S+V   + + ++ K    S+  F  + I+    S   ++ AKA      SP     PAKR
Sbjct:  352 KRVSFGGHLSPELFDKRLPPNSPLKRGATPA-----------RRSLSLV---TPLALMRK----SFGGFRQTAIK--GASPAKRSPAKAATPAKRSPAKVATPAKR 437

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BLAST of Antigen KI-67 vs. Ensembl Xenopus
Match: fbxo38 (F-box protein 38 [Source:Xenbase;Acc:XB-GENE-969087])

HSP 1 Score: 61.2326 bits (147), Expect = 4.283e-9
Identity = 27/100 (27.00%), Postives = 51/100 (51.00%), Query Frame = 3

Query:   57 GQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQS 356
            G++  + RNG +G        T   G    CDI I    +  +HC ++++ +++ F++++ S    T L  I +++P VL H  + T+     RF+ PQ+
Sbjct:    5 GEIVVIKRNGTDGTHFPLTATTCLFGRKSECDIRIQLPHVSKEHCRVEVKANKEVFVINL-STVNPTQLNGIAVQQPEVLKHGDVITIIDRSFRFEQPQT 103

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BLAST of Antigen KI-67 vs. TrEMBL
Match: A0A158QHI7 (Kin17_mid domain-containing protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS7495 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.754e-14
Identity = 74/308 (24.03%), Postives = 123/308 (39.94%), Query Frame = 3

Query:   51 INGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQF-KTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPI---------------SIAMSTPKHPMYQIHCHRTNSPTVS---------------------PIMFTNTKISTILPIKRLNVRPFVTPIQNKS----------------IDQLLQKSKLE--------------NKLL-----------------KTVSFGPNLSPETFDQEMPPAQPIKKGATP 722
            + G +  L R+G  G++ +W  +    GS  +CDI I    ++  HC L+   D + F+++V   +F +T L  + +     + HNS+ TVG     F+YP++S W  +  R    +  Q  P+               ++  S+P  P   +   R+ +PT+S                     P    N K  +IL  +  ++   V+P++ K+                I +L + S L+              N+ L                 K V FGP LSPE FD  +PPA P+K+G  P
Sbjct:  778 LRGYIVILKRDGTSGSSCEWRADKCIFGSDSSCDICIKLNSVEPFHCKLENSDDGRVFIINVA--KFSRTLLNGLGVSDRTFVAHNSLITVGDRNFTFKYPETSHWRLQPARSSVSRSAQSSPLVLSSNRLVADVSKLPTVKTSSPNRP--HLRVQRSRTPTLSSQRSGLSTRDRYCPTPTRPAVPSPDVNVKRRSILKKRTPSLEALVSPVRKKAKVLVLSETPILTGVQYIKRLRRSSSLDSFAHSNNSASFYSTNQTLNQSGASSSKLSPCHMQKKGVQFGPRLSPEQFDSRLPPATPLKRGDLP 1081

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BLAST of Antigen KI-67 vs. TrEMBL
Match: A0A402EKE1 (FHA domain-containing protein OS=Paroedura picta OX=143630 GN=parPi_0004011 PE=4 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.578e-11
Identity = 59/231 (25.54%), Postives = 97/231 (41.99%), Query Frame = 3

Query:   57 GQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSP-------IMFTNTKISTILPIKRLNVRP--FVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATP 722
            G++  + RNG +G       N+ T G    CDI I    +  +HC +++ ++++A L+++ S    T L     ++P  L H  + T+     RF+YP ++   +K                I  S  K  +  +H  +     + P       +  +  K     P KR   +P   V P+  +                K VSFG +LSPE FD+ +PP  P+KKGA P
Sbjct:  124 GKIVVIKRNGTDGTHFPLTLNSCTFGRIKECDIRIQLPQVSQEHCKIEVNENKEAILINL-STATPTQLNGRNFQQPVYLKHGDVLTIIDRSFRFEYPPATLARKK----------------IPRSEEKETLQVLHAQQVEQEKLHPQNAECKNLQNSGRKTVQKSPQKRKGGKPDLLVHPLGKR----------------KRVSFGGHLSPELFDKRLPPNSPLKKGAIP 321

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BLAST of Antigen KI-67 vs. TrEMBL
Match: A0A564YIF8 (FHA domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS6300 PE=4 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 4.947e-11
Identity = 33/104 (31.73%), Postives = 57/104 (54.81%), Query Frame = 3

Query:   54 NGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKW 365
             G +  L R+G  G++ +WH +    GS  +CD+ I  + +D  HC L+   D + F+++V  +  +T L    + +   + HNS+ TVG    +FQYP+ S+W
Sbjct:    4 RGHIVILKRDGTSGSSCEWHADKCVFGSESSCDVCIKLKSVDPFHCKLENSDDGRVFIINVAKSP-RTLLNGSGVFERTFVAHNSLITVGDRNFKFQYPEDSQW 106

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BLAST of Antigen KI-67 vs. TrEMBL
Match: A0A158QG36 (FHA domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS9915 PE=4 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 8.664e-11
Identity = 33/104 (31.73%), Postives = 57/104 (54.81%), Query Frame = 3

Query:   54 NGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKW 365
             G +  L R+G  G++ +WH +    GS  +CD+ I  + +D  HC L+   D + F+++V  +  +T L    + +   + HNS+ TVG    +FQYP+ S+W
Sbjct:  472 RGHIVILKRDGTSGSSCEWHADKCVFGSESSCDVCIKLKSVDPFHCKLENSDDGRVFIINVAKSP-RTLLNGSGVFERTFVAHNSLITVGDRNFKFQYPEDSQW 574

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BLAST of Antigen KI-67 vs. TrEMBL
Match: A0A5B7CNU1 (Antigen KI-67 OS=Portunus trituberculatus OX=210409 GN=MKI67 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.231e-10
Identity = 63/237 (26.58%), Postives = 104/237 (43.88%), Query Frame = 3

Query:   48 KINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHV--LNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNK-------SIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTT 731
            K+ G +  + R+G +G       ++ +IG +  CDI +    +  + C + +    +A+L ++  N  +T L    +    V  LNH  + +VG+   R++YP+ S                    S+A+S P                 S I   N KI  ++P+ +    P   PI N+       SID+L +  +      K VSFGP L+PE FD+ +PPA P+ KG  P +T
Sbjct:    2 KVVGYVVVIKRSGADGTPYPMVHSSCSIGRNIECDIRVQLNSVSQQQCRIDVDPSGKAYLTNMTQN-GQTMLNGYSLAPKEVCMLNHKQVLSVGERKFRWEYPEGS--------------------SLAVSAP-----------------SSIPSANYKI--LVPVDK---SPVYHPIANRGAAAHRWSIDELSEAKR------KKVSFGPQLNPEHFDKLLPPATPVSKGDKPNST 189

[Close]

BLAST of Antigen KI-67 vs. Ensembl Medaka
Match: mki67 (proliferation marker protein Ki-67 [Source:NCBI gene;Acc:101173508])

HSP 1 Score: 50.8322 bits (120), Expect = 3.462e-6
Identity = 48/152 (31.58%), Postives = 78/152 (51.32%), Query Frame = 3

Query:  456 QIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDSTKVRKRKFSIVPRGSSVGVINKDNDNSYSSFTLSDIEP-INDSFTDKAMAKARQS 908
            Q+ C +  S T+S +   N ++S    +K         P + KS +  L+K +L  +  K VSFG  LSPE FD+ +PP  P++KGATP       S  V + K +++ R S +G+I +   +   + T S  +P    S + KA + A++S
Sbjct:  273 QVQCLKCRS-TLSRMSNCNIQVSFSFSVK-------AAPKKRKSGE--LRKDQLSPQKKKRVSFGGQLSPELFDKRLPPNSPLRKGATPRR-----SLMVFRPKSTLLRRASVIGLIKQ---SPAKARTPSPKKPKAGKSASPKAASPAQKS 406

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BLAST of Antigen KI-67 vs. Planmine SMEST
Match: SMESG000050427.1 (SMESG000050427.1)

HSP 1 Score: 1058.9 bits (2737), Expect = 0.000e+0
Identity = 498/508 (98.03%), Postives = 501/508 (98.62%), Query Frame = 3

Query:   42 MEKINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDSTKVRKRKFSIVPRGSSVGVINKDNDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVPAKRRILQRNSTDPYEYSEEQEHSISPVRIKRNQSIRINSISEGLSKSVDESLNLDNIKAMENKQNQKILQIINRNEKENNVSLSNSLTKFRQLIRTSRNTNSTLDTDIDILQLNAESTTNYRFAHQNISRQSDARQSLNLQGVQELFDISEDICQEKRQFKPLHKPNHGKKSIVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            MEKINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDS KVRKRKFSIVPRGSSVGVINK NDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVPAKRRILQRNSTDPYEYSEEQEHS+SPV IKRNQSIRINS SEGLSK V ESLNLDNIKAMENKQNQKILQIINRNEKENNVSLSNSLTKFRQLIRTSRNTNSTLDTDIDILQLNAESTT YRFAHQNISRQSDARQSLNLQGVQELFDISEDICQEKRQFKP+HKPNHGKKSIVIQSDDKLYETNQKARQSCENQMKLSQ+SNRGRKS
Sbjct:    1 MEKINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDSIKVRKRKFSIVPRGSSVGVINKVNDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVPAKRRILQRNSTDPYEYSEEQEHSMSPVGIKRNQSIRINSSSEGLSKRVYESLNLDNIKAMENKQNQKILQIINRNEKENNVSLSNSLTKFRQLIRTSRNTNSTLDTDIDILQLNAESTTYYRFAHQNISRQSDARQSLNLQGVQELFDISEDICQEKRQFKPVHKPNHGKKSIVIQSDDKLYETNQKARQSCENQMKLSQKSNRGRKS 508

HSP 2 Score: 57.7658 bits (138), Expect = 2.099e-8
Identity = 38/110 (34.55%), Postives = 66/110 (60.00%), Query Frame = 3

Query: 1266 DILQLNAESTTNYRFAHQNISRQSDARQS---LNLQGVQELFDISEDICQ-EKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            +I Q+  ES+ N     Q  +R   +R+S   + +Q  +E ++I E++    ++Q    HK N G+KS      I+++S+++ YE +Q A +S ENQ+++ Q+SNRGRKS
Sbjct: 2484 EIDQVADESSQNQLVVSQKSNRGRKSRKSKAPVIIQSDEEQYEIDEEVVDFAEKQMDVSHKSNRGRKSRKSTGPIIVESEEEHYEIDQVAGESSENQLEILQKSNRGRKS 2593

HSP 3 Score: 52.373 bits (124), Expect = 9.551e-7
Identity = 29/76 (38.16%), Postives = 50/76 (65.79%), Query Frame = 3

Query: 1359 LQGVQELFDISEDICQ-EKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            +Q  +E  +I E++    ++Q    HK N G+KS      ++IQSD++ Y+ +++A +  ENQ+++SQ+SNRGRKS
Sbjct: 1687 IQSDEEQNEIDEEVVDFAEKQMDVSHKTNRGRKSRKSSAPVIIQSDEEQYKIDEEAGEFSENQLEISQKSNRGRKS 1762

HSP 4 Score: 51.2174 bits (121), Expect = 1.959e-6
Identity = 28/71 (39.44%), Postives = 46/71 (64.79%), Query Frame = 3

Query: 1371 QELFDISEDICQEKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            +E+ D +E      +Q    HK N G+KS      ++IQSD++ Y+ +++A +  ENQ+++SQ+SNRGRKS
Sbjct: 2070 EEVVDFAE------KQMDVSHKTNRGRKSRKSSAPVIIQSDEEQYKIDEEAGEFSENQLEISQKSNRGRKS 2134

HSP 5 Score: 50.8322 bits (120), Expect = 3.003e-6
Identity = 27/63 (42.86%), Postives = 43/63 (68.25%), Query Frame = 3

Query: 1395 DICQEKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            D+   ++Q    HK N G+KS      I+++S+++ YE +Q A +S ENQ+++SQ+SNRGRKS
Sbjct: 1532 DVDFAEKQMDVSHKSNPGRKSRKSTGPIIVESEEEHYEIDQVAGESSENQLEISQKSNRGRKS 1594

HSP 6 Score: 50.8322 bits (120), Expect = 3.003e-6
Identity = 27/63 (42.86%), Postives = 43/63 (68.25%), Query Frame = 3

Query: 1395 DICQEKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            D+   ++Q    HK N G+KS      I+++S+++ YE +Q A +S ENQ+++SQ+SNRGRKS
Sbjct: 1952 DVDFAEKQMDVSHKSNPGRKSRKSTGPIIVESEEEHYEIDQVAGESSENQLEISQKSNRGRKS 2014

[Close]

BLAST of Antigen KI-67 vs. Planmine SMEST
Match: SMESG000050427.1 (SMESG000050427.1)

HSP 1 Score: 1058.51 bits (2736), Expect = 0.000e+0
Identity = 498/508 (98.03%), Postives = 501/508 (98.62%), Query Frame = 3

Query:   42 MEKINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDSTKVRKRKFSIVPRGSSVGVINKDNDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVPAKRRILQRNSTDPYEYSEEQEHSISPVRIKRNQSIRINSISEGLSKSVDESLNLDNIKAMENKQNQKILQIINRNEKENNVSLSNSLTKFRQLIRTSRNTNSTLDTDIDILQLNAESTTNYRFAHQNISRQSDARQSLNLQGVQELFDISEDICQEKRQFKPLHKPNHGKKSIVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            MEKINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDS KVRKRKFSIVPRGSSVGVINK NDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVPAKRRILQRNSTDPYEYSEEQEHS+SPV IKRNQSIRINS SEGLSK V ESLNLDNIKAMENKQNQKILQIINRNEKENNVSLSNSLTKFRQLIRTSRNTNSTLDTDIDILQLNAESTT YRFAHQNISRQSDARQSLNLQGVQELFDISEDICQEKRQFKP+HKPNHGKKSIVIQSDDKLYETNQKARQSCENQMKLSQ+SNRGRKS
Sbjct:    1 MEKINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDSIKVRKRKFSIVPRGSSVGVINKVNDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVPAKRRILQRNSTDPYEYSEEQEHSMSPVGIKRNQSIRINSSSEGLSKRVYESLNLDNIKAMENKQNQKILQIINRNEKENNVSLSNSLTKFRQLIRTSRNTNSTLDTDIDILQLNAESTTYYRFAHQNISRQSDARQSLNLQGVQELFDISEDICQEKRQFKPVHKPNHGKKSIVIQSDDKLYETNQKARQSCENQMKLSQKSNRGRKS 508

HSP 2 Score: 57.7658 bits (138), Expect = 2.099e-8
Identity = 38/110 (34.55%), Postives = 66/110 (60.00%), Query Frame = 3

Query: 1266 DILQLNAESTTNYRFAHQNISRQSDARQS---LNLQGVQELFDISEDICQ-EKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            +I Q+  ES+ N     Q  +R   +R+S   + +Q  +E ++I E++    ++Q    HK N G+KS      I+++S+++ YE +Q A +S ENQ+++ Q+SNRGRKS
Sbjct: 2489 EIDQVADESSQNQLVVSQKSNRGRKSRKSKAPVIIQSDEEQYEIDEEVVDFAEKQMDVSHKSNRGRKSRKSTGPIIVESEEEHYEIDQVAGESSENQLEILQKSNRGRKS 2598

HSP 3 Score: 52.7582 bits (125), Expect = 6.556e-7
Identity = 30/79 (37.97%), Postives = 52/79 (65.82%), Query Frame = 3

Query: 1350 SLNLQGVQELFDISEDICQ-EKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            S+ +Q  +E  +I E++    ++Q    HK N G+KS      ++IQSD++ Y+ +++A +  ENQ+++SQ+SNRGRKS
Sbjct: 2061 SIIIQSDEEQNEIDEEVVDFAEKQMDVSHKTNRGRKSRKSSAPVIIQSDEEQYKIDEEAGEFSENQLEISQKSNRGRKS 2139

HSP 4 Score: 52.373 bits (124), Expect = 9.552e-7
Identity = 29/76 (38.16%), Postives = 50/76 (65.79%), Query Frame = 3

Query: 1359 LQGVQELFDISEDICQ-EKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            +Q  +E  +I E++    ++Q    HK N G+KS      ++IQSD++ Y+ +++A +  ENQ+++SQ+SNRGRKS
Sbjct: 1687 IQSDEEQNEIDEEVVDFAEKQMDVSHKTNRGRKSRKSSAPVIIQSDEEQYKIDEEAGEFSENQLEISQKSNRGRKS 1762

HSP 5 Score: 50.8322 bits (120), Expect = 3.029e-6
Identity = 27/63 (42.86%), Postives = 43/63 (68.25%), Query Frame = 3

Query: 1395 DICQEKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            D+   ++Q    HK N G+KS      I+++S+++ YE +Q A +S ENQ+++SQ+SNRGRKS
Sbjct: 1532 DVDFAEKQMDVSHKSNPGRKSRKSTGPIIVESEEEHYEIDQVAGESSENQLEISQKSNRGRKS 1594

HSP 6 Score: 50.8322 bits (120), Expect = 3.029e-6
Identity = 27/63 (42.86%), Postives = 43/63 (68.25%), Query Frame = 3

Query: 1395 DICQEKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            D+   ++Q    HK N G+KS      I+++S+++ YE +Q A +S ENQ+++SQ+SNRGRKS
Sbjct: 1952 DVDFAEKQMDVSHKSNPGRKSRKSTGPIIVESEEEHYEIDQVAGESSENQLEISQKSNRGRKS 2014

[Close]

Query:   42 MEKINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDSTKVRKRKFSIVPRGSSVGVINKDNDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVPAKRRILQRNSTDPYEYSEEQEHSISPVRIKRNQSIRINSISEGLSKSVDESLNLDNIKAMENKQNQKILQIINRNEKENNVSLSNSLTKFRQLIRTSRNTNSTLDTDIDILQLNAESTTNYRFAHQNISRQSDARQSLNLQGVQELFDISEDICQEKRQFKPLHKPNHGKKSIVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            MEKINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDS KVRKRKFSIVPRGSSVGVINK NDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVPAKRRILQRNSTDPYEYSEEQEHS+SPV IKRNQSIRINS SEGLSK V ESLNLDNIKAMENKQNQKILQIINRNEKENNVSLSNSLTKFRQLIRTSRNTNSTLDTDIDILQLNAESTT YRFAHQNISRQSDARQSLNLQGVQELFDISEDICQEKRQFKP+HKPNHGKKSIVIQSDDKLYETNQKARQSCENQMKLSQ+SNRGRKS
Sbjct:    1 MEKINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDSIKVRKRKFSIVPRGSSVGVINKVNDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVPAKRRILQRNSTDPYEYSEEQEHSMSPVGIKRNQSIRINSSSEGLSKRVYESLNLDNIKAMENKQNQKILQIINRNEKENNVSLSNSLTKFRQLIRTSRNTNSTLDTDIDILQLNAESTTYYRFAHQNISRQSDARQSLNLQGVQELFDISEDICQEKRQFKPVHKPNHGKKSIVIQSDDKLYETNQKARQSCENQMKLSQKSNRGRKS 508

HSP 2 Score: 57.7658 bits (138), Expect = 2.248e-8
Identity = 38/110 (34.55%), Postives = 66/110 (60.00%), Query Frame = 3

Query: 1266 DILQLNAESTTNYRFAHQNISRQSDARQS---LNLQGVQELFDISEDICQ-EKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            +I Q+  ES+ N     Q  +R   +R+S   + +Q  +E ++I E++    ++Q    HK N G+KS      I+++S+++ YE +Q A +S ENQ+++ Q+SNRGRKS
Sbjct: 2481 EIDQVADESSQNQLVVSQKSNRGRKSRKSKAPVIIQSDEEQYEIDEEVVDFAEKQMDVSHKSNRGRKSRKSTGPIIVESEEEHYEIDQVAGESSENQLEILQKSNRGRKS 2590

HSP 3 Score: 52.373 bits (124), Expect = 9.883e-7
Identity = 29/76 (38.16%), Postives = 50/76 (65.79%), Query Frame = 3

Query: 1359 LQGVQELFDISEDICQ-EKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            +Q  +E  +I E++    ++Q    HK N G+KS      ++IQSD++ Y+ +++A +  ENQ+++SQ+SNRGRKS
Sbjct: 1687 IQSDEEQNEIDEEVVDFAEKQMDVSHKTNRGRKSRKSSAPVIIQSDEEQYKIDEEAGEFSENQLEISQKSNRGRKS 1762

HSP 4 Score: 52.373 bits (124), Expect = 9.883e-7
Identity = 29/76 (38.16%), Postives = 50/76 (65.79%), Query Frame = 3

Query: 1359 LQGVQELFDISEDICQ-EKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            +Q  +E  +I E++    ++Q    HK N G+KS      ++IQSD++ Y+ +++A +  ENQ+++SQ+SNRGRKS
Sbjct: 2056 IQSDEEQNEIDEEVVDFAEKQMDVSHKTNRGRKSRKSSAPVIIQSDEEQYKIDEEAGEFSENQLEISQKSNRGRKS 2131

HSP 5 Score: 50.8322 bits (120), Expect = 3.134e-6
Identity = 27/63 (42.86%), Postives = 43/63 (68.25%), Query Frame = 3

Query: 1395 DICQEKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            D+   ++Q    HK N G+KS      I+++S+++ YE +Q A +S ENQ+++SQ+SNRGRKS
Sbjct: 1532 DVDFAEKQMDVSHKSNPGRKSRKSTGPIIVESEEEHYEIDQVAGESSENQLEISQKSNRGRKS 1594

HSP 6 Score: 50.8322 bits (120), Expect = 3.134e-6
Identity = 27/63 (42.86%), Postives = 43/63 (68.25%), Query Frame = 3

Query: 1395 DICQEKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            D+   ++Q    HK N G+KS      I+++S+++ YE +Q A +S ENQ+++SQ+SNRGRKS
Sbjct: 1952 DVDFAEKQMDVSHKSNPGRKSRKSTGPIIVESEEEHYEIDQVAGESSENQLEISQKSNRGRKS 2014

[Close]

BLAST of Antigen KI-67 vs. Planmine SMEST
Match: SMESG000050427.1 (SMESG000050427.1)

HSP 1 Score: 1058.13 bits (2735), Expect = 0.000e+0
Identity = 498/508 (98.03%), Postives = 501/508 (98.62%), Query Frame = 3

Query:   42 MEKINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDSTKVRKRKFSIVPRGSSVGVINKDNDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVPAKRRILQRNSTDPYEYSEEQEHSISPVRIKRNQSIRINSISEGLSKSVDESLNLDNIKAMENKQNQKILQIINRNEKENNVSLSNSLTKFRQLIRTSRNTNSTLDTDIDILQLNAESTTNYRFAHQNISRQSDARQSLNLQGVQELFDISEDICQEKRQFKPLHKPNHGKKSIVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            MEKINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDS KVRKRKFSIVPRGSSVGVINK NDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVPAKRRILQRNSTDPYEYSEEQEHS+SPV IKRNQSIRINS SEGLSK V ESLNLDNIKAMENKQNQKILQIINRNEKENNVSLSNSLTKFRQLIRTSRNTNSTLDTDIDILQLNAESTT YRFAHQNISRQSDARQSLNLQGVQELFDISEDICQEKRQFKP+HKPNHGKKSIVIQSDDKLYETNQKARQSCENQMKLSQ+SNRGRKS
Sbjct:    1 MEKINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHCILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRFQYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTVSPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTVSFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDSIKVRKRKFSIVPRGSSVGVINKVNDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVPAKRRILQRNSTDPYEYSEEQEHSMSPVGIKRNQSIRINSSSEGLSKRVYESLNLDNIKAMENKQNQKILQIINRNEKENNVSLSNSLTKFRQLIRTSRNTNSTLDTDIDILQLNAESTTYYRFAHQNISRQSDARQSLNLQGVQELFDISEDICQEKRQFKPVHKPNHGKKSIVIQSDDKLYETNQKARQSCENQMKLSQKSNRGRKS 508

HSP 2 Score: 57.7658 bits (138), Expect = 2.064e-8
Identity = 38/110 (34.55%), Postives = 66/110 (60.00%), Query Frame = 3

Query: 1266 DILQLNAESTTNYRFAHQNISRQSDARQS---LNLQGVQELFDISEDICQ-EKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            +I Q+  ES+ N     Q  +R   +R+S   + +Q  +E ++I E++    ++Q    HK N G+KS      I+++S+++ YE +Q A +S ENQ+++ Q+SNRGRKS
Sbjct: 2491 EIDQVADESSQNQLVVSQKSNRGRKSRKSKAPVIIQSDEEQYEIDEEVVDFAEKQMDVSHKSNRGRKSRKSTGPIIVESEEEHYEIDQVAGESSENQLEILQKSNRGRKS 2600

HSP 3 Score: 52.373 bits (124), Expect = 9.310e-7
Identity = 29/76 (38.16%), Postives = 50/76 (65.79%), Query Frame = 3

Query: 1359 LQGVQELFDISEDICQ-EKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            +Q  +E  +I E++    ++Q    HK N G+KS      ++IQSD++ Y+ +++A +  ENQ+++SQ+SNRGRKS
Sbjct: 1687 IQSDEEQNEIDEEVVDFAEKQMDVSHKTNRGRKSRKSSAPVIIQSDEEQYKIDEEAGEFSENQLEISQKSNRGRKS 1762

HSP 4 Score: 52.373 bits (124), Expect = 9.310e-7
Identity = 29/76 (38.16%), Postives = 50/76 (65.79%), Query Frame = 3

Query: 1359 LQGVQELFDISEDICQ-EKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            +Q  +E  +I E++    ++Q    HK N G+KS      ++IQSD++ Y+ +++A +  ENQ+++SQ+SNRGRKS
Sbjct: 2066 IQSDEEQNEIDEEVVDFAEKQMDVSHKTNRGRKSRKSSAPVIIQSDEEQYKIDEEAGEFSENQLEISQKSNRGRKS 2141

HSP 5 Score: 50.8322 bits (120), Expect = 3.003e-6
Identity = 27/63 (42.86%), Postives = 43/63 (68.25%), Query Frame = 3

Query: 1395 DICQEKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            D+   ++Q    HK N G+KS      I+++S+++ YE +Q A +S ENQ+++SQ+SNRGRKS
Sbjct: 1532 DVDFAEKQMDVSHKSNPGRKSRKSTGPIIVESEEEHYEIDQVAGESSENQLEISQKSNRGRKS 1594

HSP 6 Score: 50.8322 bits (120), Expect = 3.003e-6
Identity = 27/63 (42.86%), Postives = 43/63 (68.25%), Query Frame = 3

Query: 1395 DICQEKRQFKPLHKPNHGKKS------IVIQSDDKLYETNQKARQSCENQMKLSQQSNRGRKS 1565
            D+   ++Q    HK N G+KS      I+++S+++ YE +Q A +S ENQ+++SQ+SNRGRKS
Sbjct: 1952 DVDFAEKQMDVSHKSNPGRKSRKSTGPIIVESEEEHYEIDQVAGESSENQLEISQKSNRGRKS 2014

The following BLAST results are available for this feature:

BLAST of Antigen KI-67 vs. Ensembl Human
BLAST of Antigen KI-67 vs. Ensembl Celegans
BLAST of Antigen KI-67 vs. Ensembl Fly
BLAST of Antigen KI-67 vs. Ensembl Zebrafish
BLAST of Antigen KI-67 vs. Ensembl Xenopus
BLAST of Antigen KI-67 vs. Ensembl Mouse
BLAST of Antigen KI-67 vs. UniProt/SwissProt
BLAST of Antigen KI-67 vs. TrEMBL
BLAST of Antigen KI-67 vs. Ensembl Cavefish
BLAST of Antigen KI-67 vs. Ensembl Sea Lamprey
BLAST of Antigen KI-67 vs. Ensembl Yeast
BLAST of Antigen KI-67 vs. Ensembl Nematostella
BLAST of Antigen KI-67 vs. Ensembl Medaka
BLAST of Antigen KI-67 vs. Planmine SMEST

BLAST of Antigen KI-67 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Antigen KI-67 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Antigen KI-67 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Antigen KI-67 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Antigen KI-67 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2


Match Name	E-value	Identity	Description
fbxo38	3.954e-9	27.00	F-box protein 38 [Source:Xenbase;Acc:XB-GENE-96908...	[more]
fbxo38	4.283e-9	27.00	F-box protein 38 [Source:Xenbase;Acc:XB-GENE-96908...	[more]

BLAST of Antigen KI-67 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Antigen KI-67 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Antigen KI-67 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A158QHI7	1.754e-14	24.03	Kin17_mid domain-containing protein OS=Rodentolepi...	[more]
A0A402EKE1	3.578e-11	25.54	FHA domain-containing protein OS=Paroedura picta O...	[more]
A0A564YIF8	4.947e-11	31.73	FHA domain-containing protein OS=Hymenolepis dimin...	[more]
A0A158QG36	8.664e-11	31.73	FHA domain-containing protein OS=Hymenolepis dimin...	[more]
A0A5B7CNU1	1.231e-10	26.58	Antigen KI-67 OS=Portunus trituberculatus OX=21040...	[more]

BLAST of Antigen KI-67 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Antigen KI-67 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Antigen KI-67 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Antigen KI-67 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Antigen KI-67 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
mki67	3.462e-6	31.58	proliferation marker protein Ki-67 [Source:NCBI ge...	[more]

BLAST of Antigen KI-67 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4


Match Name	E-value	Identity	Description
SMESG000050427.1	2.099e-8	98.03	SMESG000050427.1	[more]
SMESG000050427.1	2.099e-8	98.03	SMESG000050427.1	[more]
SMESG000050427.1	2.248e-8	98.03	SMESG000050427.1	[more]
SMESG000050427.1	2.064e-8	98.03	SMESG000050427.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30034422 ID=SMED30034422|Name=Antigen KI-67|organism=Schmidtea mediterranea sexual|type=transcript|length=1566bp
GATTTTAGTAATATTTATTTATAATCTTTATTTCATTTTAGATGGAAAAG
ATAAATGGACAGCTAACATTTTTAAACCGAAATGGATTAGAAGGTGCAAC
AATAAAATGGCATAAAAACACTTTTACAATTGGAAGCCACGATAATTGTG
ATATTTCTATTGTGTCTGAATTTATTGATTCAAAGCACTGTATTTTAAAA
CTACAAAAGGACGAACAAGCTTTTTTAGTGCACGTAGGGTCAAATCAATT
TAAAACATATTTAGAAGACATTGAAATTAAAAAACCCCATGTGCTAAATC
ATAATTCAATTTTCACAGTAGGAAAATCTTTTATTCGTTTTCAATATCCA
CAATCTTCAAAATGGTTTCGAAAAGAGAATCGTCACATTGAAGGTCAGAT
AGGTCAGCAATTTCCAATTTCTATAGCTATGTCAACCCCAAAACATCCAA
TGTATCAAATACATTGTCATAGAACAAATAGTCCAACAGTTTCACCAATA
ATGTTCACTAACACGAAAATTAGCACGATATTGCCCATTAAACGATTGAA
TGTACGCCCATTCGTAACTCCAATTCAAAATAAGTCGATCGACCAACTTC
TACAAAAAAGTAAATTGGAAAACAAATTATTGAAAACCGTATCATTTGGG
CCTAATTTGAGCCCTGAAACATTTGATCAGGAAATGCCACCAGCTCAACC
AATTAAAAAAGGCGCAACTCCGATGACAACGGCCGATTCCGATAGCACCA
AAGTCAGGAAAAGAAAATTCAGTATTGTTCCGCGTGGAAGTTCTGTTGGT
GTTATTAACAAGGATAACGACAATTCCTATTCATCCTTCACTCTTTCTGA
CATCGAACCAATCAATGATTCATTCACCGATAAGGCAATGGCAAAAGCCC
GTCAATCTAATGTCGAATCACCAATACATTATATGGTGCCAGCAAAACGC
AGAATCTTACAGAGGAATTCAACAGATCCATACGAATATTCAGAGGAACA
AGAGCATTCGATATCTCCAGTCAGGATCAAAAGAAACCAATCGATTCGTA
TAAACTCTATTAGTGAAGGCCTTTCAAAAAGTGTTGACGAAAGCTTAAAT
TTGGACAATATTAAGGCAATGGAAAATAAACAAAATCAGAAAATATTACA
AATCATCAATAGAAATGAAAAGGAGAATAATGTTTCTCTTTCAAATAGTC
TCACAAAATTCAGACAGCTTATCAGGACTTCAAGAAACACAAATTCAACA
TTGGATACTGATATCGACATACTACAGCTCAATGCGGAATCAACGACTAA
TTATCGATTTGCCCATCAAAACATTTCACGTCAATCCGATGCGAGGCAGT
CTTTAAATCTTCAAGGAGTCCAAGAGCTATTTGATATTTCAGAAGATATT
TGTCAAGAAAAGAGACAATTTAAACCTCTTCACAAACCAAACCATGGAAA
AAAATCAATTGTTATCCAATCAGACGATAAATTATATGAAACGAATCAAA
AGGCGCGACAATCATGCGAAAATCAAATGAAACTTTCACAACAATCGAAC
CGTGGAAGAAAGTCTC

protein sequence of SMED30034422-orf-1

>SMED30034422-orf-1 ID=SMED30034422-orf-1|Name=SMED30034422-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=508bp
MEKINGQLTFLNRNGLEGATIKWHKNTFTIGSHDNCDISIVSEFIDSKHC
ILKLQKDEQAFLVHVGSNQFKTYLEDIEIKKPHVLNHNSIFTVGKSFIRF
QYPQSSKWFRKENRHIEGQIGQQFPISIAMSTPKHPMYQIHCHRTNSPTV
SPIMFTNTKISTILPIKRLNVRPFVTPIQNKSIDQLLQKSKLENKLLKTV
SFGPNLSPETFDQEMPPAQPIKKGATPMTTADSDSTKVRKRKFSIVPRGS
SVGVINKDNDNSYSSFTLSDIEPINDSFTDKAMAKARQSNVESPIHYMVP
AKRRILQRNSTDPYEYSEEQEHSISPVRIKRNQSIRINSISEGLSKSVDE
SLNLDNIKAMENKQNQKILQIINRNEKENNVSLSNSLTKFRQLIRTSRNT
NSTLDTDIDILQLNAESTTNYRFAHQNISRQSDARQSLNLQGVQELFDIS
EDICQEKRQFKPLHKPNHGKKSIVIQSDDKLYETNQKARQSCENQMKLSQ
QSNRGRKS

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000014	zeta neoblast
PLANA:0000420	parapharyngeal region
PLANA:0000429	neoblast
PLANA:0002075	pigment cell
PLANA:0002109	X1 cell
PLANA:0002111	X2 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR000253	FHA_dom
IPR029334	PP1-bd
IPR008984	SMAD_FHA_dom_sf

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding
GO:0003779	actin binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:2.60.200.20		coord: 1..102 e-value: 3.0E-15 score: 58.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 484..508
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 487..501
IPR029334	Protein phosphatase 1 binding domain	PFAM	PF15276	PP1_bind	coord: 198..238 e-value: 2.4E-10 score: 40.5
IPR000253	Forkhead-associated (FHA) domain	PFAM	PF00498	FHA	coord: 29..94 e-value: 2.7E-7 score: 30.9
IPR000253	Forkhead-associated (FHA) domain	PROSITE	PS50006	FHA_DOMAIN	coord: 28..79 score: 8.159
IPR000253	Forkhead-associated (FHA) domain	CDD	cd00060	FHA	coord: 25..102 e-value: 3.80057E-8 score: 49.307
IPR008984	SMAD/FHA domain superfamily	SUPERFAMILY	SSF49879	SMAD/FHA domain	coord: 12..103

Main menu

Antigen KI-67

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 38

Owlery

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