Calpain
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30034337 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of Calpain vs. Ensembl Human
Match: CAPN9 (calpain 9 [Source:HGNC Symbol;Acc:HGNC:1486]) HSP 1 Score: 190.274 bits (482), Expect = 3.195e-51 Identity = 98/213 (46.01%), Postives = 135/213 (63.38%), Query Frame = 1
Query: 160 SKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPIN 795
S + F ++ L + LF D F SN L+ S F WKRP EI KNP+FI G +R D+ QG LGDCWLLAAI SL+L+ + +VI DQ F Y G F F+ W+ W DVV+DDRLPT +LV++HS++ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + F+ +K +P ++++ KA S++ C I+
Sbjct: 25 SGQSFEQMRQECLQRGTLFEDADFPASNSSLFYSERPQIPFVWKRPGEIVKNPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVIPQDQSFGPGYAGIFHFQFWQHSEWLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAETFQ--TKEAPENFYEILEKALKRGSLLGCFID 235 HSP 2 Score: 110.923 bits (276), Expect = 1.031e-24 Identity = 77/266 (28.95%), Postives = 128/266 (48.12%), Query Frame = 1
Query: 1066 TPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI--NTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK-LCETIICITMLEGSLVNMPDSSFI---YSIFKIEDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
+ + E + GLI+GHAYSVT + +V +I L+RIRNPWG +VEW G++SD M+F+DF +F +EIC +T D +W+ G W + + D F NPQ + ++ EKD G+ C ++ + + + ++ + Y+I++ D + L+ +FF+ ++S + + F++ + R +LPPG Y++IPST EADF LRI E + +D
Sbjct: 238 SAAESEARTPFGLIKGHAYSVTGIDQVSFRGQRIELIRIRNPWG-QVEWNGSWSDR--------------------------MAFKDFKAHFDKVEICNLTPDALEEDAIHKWEVTVHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSL--TEKDEGQEECSFLVALMQKDRRKLKRFGANVLTIGYAIYECPDK---DEHLNKDFFRYHASRARSKTFINLREVSDRFKLPPGEYILIPSTFEPHQEADFCLRIFSEKKAITRDMD 471
BLAST of Calpain vs. Ensembl Human
Match: CAPN3 (calpain 3 [Source:HGNC Symbol;Acc:HGNC:1480]) HSP 1 Score: 190.66 bits (483), Expect = 5.186e-51 Identity = 100/211 (47.39%), Postives = 134/211 (63.51%), Query Frame = 1
Query: 163 KKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
+K F K L K L+ DP F P L+ S +F WKRP EI +NP+FI DG +R D+ QG LGDCW LAAI L+L+ +L+ +VI DQ F ++Y G F F+ WR G W DVV+DD LPT N +LV+ S++ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + F + A ++ +M KA S+M C I+
Sbjct: 58 EKTFEQLHKKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVAEFFEI-RDAPSDMYKIMKKAIERGSLMGCSID 267 HSP 2 Score: 150.984 bits (380), Expect = 1.668e-37 Identity = 95/282 (33.69%), Postives = 150/282 (53.19%), Query Frame = 1
Query: 1069 PVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK---LCETIICITM--------LEGSLVNMPDSSFIYSIFKIEDARYLNAQ-LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPI 1848
PVQ E ++ GL+ GHAYSVT + EV ++ LVR+RNPWG +VEW G++SD +W+ +++ E+ L+ DGEFWMS++DFI +F+ LEIC +T+D +K W G+W + + D F NPQYR +L + DP +C ++ + L SL + ++I+++ + N Q L +FF N+S + + +++ + R RLPP YVI+PST E +F+LR+ E E V+ T+ P+
Sbjct: 271 PVQYETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRLRNPWG-QVEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGASLFTIG-----FAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPV 546
BLAST of Calpain vs. Ensembl Human
Match: CAPN3 (calpain 3 [Source:HGNC Symbol;Acc:HGNC:1480]) HSP 1 Score: 191.045 bits (484), Expect = 5.817e-51 Identity = 100/211 (47.39%), Postives = 134/211 (63.51%), Query Frame = 1
Query: 163 KKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
+K F K L K L+ DP F P L+ S +F WKRP EI +NP+FI DG +R D+ QG LGDCW LAAI L+L+ +L+ +VI DQ F ++Y G F F+ WR G W DVV+DD LPT N +LV+ S++ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + F + A ++ +M KA S+M C I+
Sbjct: 58 EKTFEQLHKKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVAEFFEI-RDAPSDMYKIMKKAIERGSLMGCSID 267 HSP 2 Score: 151.369 bits (381), Expect = 1.644e-37 Identity = 93/277 (33.57%), Postives = 148/277 (53.43%), Query Frame = 1
Query: 1069 PVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK---LCETIICITMLEGSLVNMPDSSFI---YSIFKIEDARYLNAQ-LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPI 1848
PVQ E ++ GL+ GHAYSVT + EV ++ LVR+RNPWG +VEW G++SD +W+ +++ E+ L+ DGEFWMS++DFI +F+ LEIC +T+D +K W G+W + + D F NPQYR +L + DP +C ++ + +S ++I+++ + N Q L +FF N+S + + +++ + R RLPP YVI+PST E +F+LR+ E E V+ T+ P+
Sbjct: 271 PVQYETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRLRNPWG-QVEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGASLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPV 546
BLAST of Calpain vs. Ensembl Human
Match: CAPN9 (calpain 9 [Source:HGNC Symbol;Acc:HGNC:1486]) HSP 1 Score: 189.889 bits (481), Expect = 6.585e-51 Identity = 98/213 (46.01%), Postives = 135/213 (63.38%), Query Frame = 1
Query: 160 SKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPIN 795
S + F ++ L + LF D F SN L+ S F WKRP EI KNP+FI G +R D+ QG LGDCWLLAAI SL+L+ + +VI DQ F Y G F F+ W+ W DVV+DDRLPT +LV++HS++ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + F+ +K +P ++++ KA S++ C I+
Sbjct: 25 SGQSFEQMRQECLQRGTLFEDADFPASNSSLFYSERPQIPFVWKRPGEIVKNPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVIPQDQSFGPGYAGIFHFQFWQHSEWLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAETFQ--TKEAPENFYEILEKALKRGSLLGCFID 235 HSP 2 Score: 150.214 bits (378), Expect = 2.574e-37 Identity = 89/266 (33.46%), Postives = 145/266 (54.51%), Query Frame = 1
Query: 1066 TPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI--NTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK-LCETIICITMLEGSLVNMPDSSFI---YSIFKIEDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
+ + E + GLI+GHAYSVT + +V +I L+RIRNPWG +VEW G++SD+SPEW ++ E+K L +DGEFWM+F+DF +F +EIC +T D +W+ G W + + D F NPQ + ++ EKD G+ C ++ + + + ++ + Y+I++ D + L+ +FF+ ++S + + F++ + R +LPPG Y++IPST EADF LRI E + +D
Sbjct: 238 SAAESEARTPFGLIKGHAYSVTGIDQVSFRGQRIELIRIRNPWG-QVEWNGSWSDSSPEWRSVGPAEQKRLCHTALDDGEFWMAFKDFKAHFDKVEICNLTPDALEEDAIHKWEVTVHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSL--TEKDEGQEECSFLVALMQKDRRKLKRFGANVLTIGYAIYECPDK---DEHLNKDFFRYHASRARSKTFINLREVSDRFKLPPGEYILIPSTFEPHQEADFCLRIFSEKKAITRDMD 497
BLAST of Calpain vs. Ensembl Human
Match: CAPN3 (calpain 3 [Source:HGNC Symbol;Acc:HGNC:1480]) HSP 1 Score: 191.43 bits (485), Expect = 6.686e-51 Identity = 100/211 (47.39%), Postives = 134/211 (63.51%), Query Frame = 1
Query: 163 KKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
+K F K L K L+ DP F P L+ S +F WKRP EI +NP+FI DG +R D+ QG LGDCW LAAI L+L+ +L+ +VI DQ F ++Y G F F+ WR G W DVV+DD LPT N +LV+ S++ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + F + A ++ +M KA S+M C I+
Sbjct: 58 EKTFEQLHKKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVAEFFEI-RDAPSDMYKIMKKAIERGSLMGCSID 267 HSP 2 Score: 151.369 bits (381), Expect = 1.810e-37 Identity = 93/277 (33.57%), Postives = 148/277 (53.43%), Query Frame = 1
Query: 1069 PVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK---LCETIICITMLEGSLVNMPDSSFI---YSIFKIEDARYLNAQ-LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPI 1848
PVQ E ++ GL+ GHAYSVT + EV ++ LVR+RNPWG +VEW G++SD +W+ +++ E+ L+ DGEFWMS++DFI +F+ LEIC +T+D +K W G+W + + D F NPQYR +L + DP +C ++ + +S ++I+++ + N Q L +FF N+S + + +++ + R RLPP YVI+PST E +F+LR+ E E V+ T+ P+
Sbjct: 319 PVQYETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRLRNPWG-QVEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGASLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPV 594
BLAST of Calpain vs. Ensembl Celegans
Match: clp-4 (CaLPain family [Source:UniProtKB/TrEMBL;Acc:Q8IAA9]) HSP 1 Score: 194.897 bits (494), Expect = 1.125e-53 Identity = 94/208 (45.19%), Postives = 128/208 (61.54%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
F ++++ L LF DP F + LY + D+ WKRP EI NPQ I G SRFDV QG LGDCW LAA+ +++L L +++ +Q F ++Y G F F+ W G W DVVVDDRLPT N +L Y+HS++ EFWSAL+EKA+AKL G Y+NLDGG + +A+E TGG+ + F L + +VK M S+ C I+
Sbjct: 227 FETEREKCLRNKTLFEDPEFPATAASLYYRTPPRDRIIWKRPGEIIANPQLITQGESRFDVKQGALGDCWFLAALANITLYDALFYRIVPPNQSFTENYAGIFHFQFWHYGKWVDVVVDDRLPTVNNQLYYLHSADNTEFWSALVEKAYAKLHGGYENLDGGTTAEALEDFTGGLTEYFDLRKSEKAAVLAALVKGMEMGSLFGCSID 434 HSP 2 Score: 113.235 bits (282), Expect = 6.333e-26 Identity = 54/133 (40.60%), Postives = 79/133 (59.40%), Query Frame = 1
Query: 1078 REIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI------NTG----------NKQWKTYNEFGKWS 1428
+E ++ GL+ GHAYS+T + + L+R+RNPWGNE EW GA+SD S EW+ I+E +K +++ F DGEFWMSF+DF + F+ +E+C +T++I TG QW E G+WS
Sbjct: 440 KEAQLRNGLVCGHAYSITAIHSITYYGEDTTLLRLRNPWGNEKEWNGAWSDGSSEWSKIDEATKKQIDVQFARDGEFWMSFEDFFSNFTQMEVCNLTAEIFDEIAEMTGVNRATETVEEEHQWHEIMEDGEWS 572
BLAST of Calpain vs. Ensembl Celegans
Match: clp-4 (CaLPain family [Source:UniProtKB/TrEMBL;Acc:Q8IAA9]) HSP 1 Score: 196.052 bits (497), Expect = 2.229e-53 Identity = 94/208 (45.19%), Postives = 128/208 (61.54%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
F ++++ L LF DP F + LY + D+ WKRP EI NPQ I G SRFDV QG LGDCW LAA+ +++L L +++ +Q F ++Y G F F+ W G W DVVVDDRLPT N +L Y+HS++ EFWSAL+EKA+AKL G Y+NLDGG + +A+E TGG+ + F L + +VK M S+ C I+
Sbjct: 227 FETEREKCLRNKTLFEDPEFPATAASLYYRTPPRDRIIWKRPGEIIANPQLITQGESRFDVKQGALGDCWFLAALANITLYDALFYRIVPPNQSFTENYAGIFHFQFWHYGKWVDVVVDDRLPTVNNQLYYLHSADNTEFWSALVEKAYAKLHGGYENLDGGTTAEALEDFTGGLTEYFDLRKSEKAAVLAALVKGMEMGSLFGCSID 434 HSP 2 Score: 140.969 bits (354), Expect = 1.037e-34 Identity = 88/276 (31.88%), Postives = 135/276 (48.91%), Query Frame = 1
Query: 1078 REIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI------NTG----------NKQWKTYNEFGKWSQNLGS--------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLE---------GSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPEL 1803
+E ++ GL+ GHAYS+T + + L+R+RNPWGNE EW GA+SD S EW+ I+E +K +++ F DGEFWMSF+DF + F+ +E+C +T++I TG QW E G+WS G+ + NPQ+ + + + +L+ G V +P IYS+ AR + L ++FF N + F++ + R R+PPG YVI+P T +A+FLLR+ L
Sbjct: 440 KEAQLRNGLVCGHAYSITAIHSITYYGEDTTLLRLRNPWGNEKEWNGAWSDGSSEWSKIDEATKKQIDVQFARDGEFWMSFEDFFSNFTQMEVCNLTAEIFDEIAEMTGVNRATETVEEEHQWHEIMEDGEWSSKKGTAGGCNNNPSTYPKNPQFSTFFTAPQSSIEADGNVTVIVAVLQKYRRELRSKGKDV-LPIGVSIYSLGAEGTAR---SPLTAQFFSQNRPIARTTVFVNTREVTVRFRVPPGQYVIVPCTFDAYDDAEFLLRVYANGTL 711
BLAST of Calpain vs. Ensembl Celegans
Match: clp-7 (CaLPain family [Source:UniProtKB/TrEMBL;Acc:U4PMH7]) HSP 1 Score: 196.052 bits (497), Expect = 3.688e-53 Identity = 95/208 (45.67%), Postives = 130/208 (62.50%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
F +++ L + LF DP F ++ LY + + WKRP EI +NPQ I +SRFDV QG LGDCWLLAA+ +L+L L +V+ DQ F ++Y G F F+ WR G W DVV+DDRLPT +G+L YM S +EFWSALLEKA+AKL G Y+NLDGG + +A+E TGG+ + F + ++V+ M S+ C I+
Sbjct: 297 FQQERQRCLDQRCLFEDPQFPANDSSLYYKTRPDEPIIWKRPGEIYENPQLIVGEKSRFDVKQGALGDCWLLAAVANLTLRDELFYRVVPPDQSFTENYAGIFHFQFWRYGQWVDVVIDDRLPTVDGRLCYMRSQENNEFWSALLEKAYAKLYGGYENLDGGTTAEALEDFTGGLTEFFDISKGDKSTTLAMLVRGMQMGSLFGCSID 504 HSP 2 Score: 143.665 bits (361), Expect = 1.869e-35 Identity = 85/270 (31.48%), Postives = 135/270 (50.00%), Query Frame = 1
Query: 1078 REIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI-------------NTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYR--FTILVNEKDPGKLCETIICI------TMLEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKYEFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPEL 1803
+E ++ GL+ GHAYS+T ++ V + GQ+ L+RIRNPWGN EW GA+SD S EW+ ++ +R+ + + F DGEFWMSF DF+T F+ +E C +++D+ + QW +E G+W+ G+ D + NPQY F + ++ + C I + + L N+P +S++K + + Q + F++ + R R+PPG YVI+P T +A FLLRI E
Sbjct: 510 KEAQLTNGLVRGHAYSITAIQTVNTYGGQVPLLRIRNPWGNSKEWNGAWSDGSSEWSQVDPQQREQMGVQFAKDGEFWMSFDDFMTNFTQMECCNLSADVMDEISEMTGVEVHDKQKHQWVEKSEDGEWNSRQGTAGGCQNNDTYCNNPQYGTYFQVPMDSVEHDGKCTVIGAVLQKYRRELRTKGLDNLP---IGFSVYKADGS----GQAIHDVSGQKPIARTKVFINMREVTVRFRVPPGQYVIVPCTFDAHDDASFLLRIFSNAEF 772
BLAST of Calpain vs. Ensembl Celegans
Match: clp-7 (CaLPain family [Source:UniProtKB/TrEMBL;Acc:U4PMH7]) HSP 1 Score: 196.052 bits (497), Expect = 3.688e-53 Identity = 95/208 (45.67%), Postives = 130/208 (62.50%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
F +++ L + LF DP F ++ LY + + WKRP EI +NPQ I +SRFDV QG LGDCWLLAA+ +L+L L +V+ DQ F ++Y G F F+ WR G W DVV+DDRLPT +G+L YM S +EFWSALLEKA+AKL G Y+NLDGG + +A+E TGG+ + F + ++V+ M S+ C I+
Sbjct: 297 FQQERQRCLDQRCLFEDPQFPANDSSLYYKTRPDEPIIWKRPGEIYENPQLIVGEKSRFDVKQGALGDCWLLAAVANLTLRDELFYRVVPPDQSFTENYAGIFHFQFWRYGQWVDVVIDDRLPTVDGRLCYMRSQENNEFWSALLEKAYAKLYGGYENLDGGTTAEALEDFTGGLTEFFDISKGDKSTTLAMLVRGMQMGSLFGCSID 504 HSP 2 Score: 143.665 bits (361), Expect = 1.869e-35 Identity = 85/270 (31.48%), Postives = 135/270 (50.00%), Query Frame = 1
Query: 1078 REIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI-------------NTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYR--FTILVNEKDPGKLCETIICI------TMLEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKYEFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPEL 1803
+E ++ GL+ GHAYS+T ++ V + GQ+ L+RIRNPWGN EW GA+SD S EW+ ++ +R+ + + F DGEFWMSF DF+T F+ +E C +++D+ + QW +E G+W+ G+ D + NPQY F + ++ + C I + + L N+P +S++K + + Q + F++ + R R+PPG YVI+P T +A FLLRI E
Sbjct: 510 KEAQLTNGLVRGHAYSITAIQTVNTYGGQVPLLRIRNPWGNSKEWNGAWSDGSSEWSQVDPQQREQMGVQFAKDGEFWMSFDDFMTNFTQMECCNLSADVMDEISEMTGVEVHDKQKHQWVEKSEDGEWNSRQGTAGGCQNNDTYCNNPQYGTYFQVPMDSVEHDGKCTVIGAVLQKYRRELRTKGLDNLP---IGFSVYKADGS----GQAIHDVSGQKPIARTKVFINMREVTVRFRVPPGQYVIVPCTFDAHDDASFLLRIFSNAEF 772
BLAST of Calpain vs. Ensembl Celegans
Match: clp-6 (CaLPain family [Source:UniProtKB/TrEMBL;Acc:Q9N4B2]) HSP 1 Score: 191.43 bits (485), Expect = 2.519e-52 Identity = 96/223 (43.05%), Postives = 135/223 (60.54%), Query Frame = 1
Query: 169 WFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN----HSQVQLAQKL 825
+F +++ L +N+LF DP F +N +Y W RP +I NP+ I +G+SRFDV QG LG+CW LAA +L+ L +V+ DQ F ++Y G F F+ WR G W DVV+DDRLPT NGKL+YM S + DE W LLEKA+AKL G+Y++LDGG + +A+E TGG+ + + L S I +++K M S+ C I+ + QLA L
Sbjct: 143 YFYEERQKCLDENRLFEDPQFPANNSSIYYKKPPKSHIVWLRPAKIFANPRLIVNGKSRFDVRQGSLGNCWFLAAAATLAQRDELFYRVVPPDQSFTENYAGIFHFQFWRYGEWVDVVIDDRLPTVNGKLIYMSSQDGDECWGPLLEKAYAKLYGTYEHLDGGTTTEALEDFTGGLTEFYDLTSTDKTMILAMIMKGMQMGSLFACSIDPDPREKEAQLANGL 365 HSP 2 Score: 123.25 bits (308), Expect = 4.865e-29 Identity = 80/270 (29.63%), Postives = 126/270 (46.67%), Query Frame = 1
Query: 1069 PVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI-------------NTGNKQWKTYNEFGKWSQNLGS--------DNFHLNPQYRFTILVNEKDPGKLCETIICI--TMLEGSLVNMPDSSFI-YSIFKIEDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPEL 1803
P ++E ++ GL+ GHAYSVT V V + + ++ L+RIRNPWG+ EW G +SD S W +++ +R+ +E DGEFWM DF+ F++L+ C +++D+ +KQW + G+W G+ + F NPQY C I + L D I S++K+ N ++ +E KT++ + F+ R +PPG YVI+P T +A+FLLR E
Sbjct: 354 PREKEAQLANGLVRGHAYSVTGVHTVETDKEKVALLRIRNPWGD-TEWNGDWSDKSSLWEQVDQEQREKMEFRIKEDGEFWMCLDDFMAQFANLDCCNLSADVMHEITEMTEIEVMEKQSKQWIQKSADGEWCSRKGTAGGCSNNENTFCTNPQYETYFRATSPSSNDKCTVIAAVFQKYRRNQLHRGLDMLQIGLSVYKMSGP---NEKVTAEMMKTHAPIASTKLFVDYREAVVRFTVPPGYYVIVPCTFEPNHDAEFLLRTFSNVEF 619
BLAST of Calpain vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0086530) HSP 1 Score: 202.216 bits (513), Expect = 1.728e-56 Identity = 103/214 (48.13%), Postives = 140/214 (65.42%), Query Frame = 1
Query: 196 LSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPI----NHSQVQLAQKL 825
L+ LF DP F SN+ L S +W RP EI +NPQF +G SRFDV QG LGDCWLLAA +L+ +SNL +VI +Q F+++Y G F FR W+ G W DV++DDRLPT NG+L+YMHS+ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + + L +A ++ ++ KA+ S+M C I N ++ + Q L
Sbjct: 83 LASGSLFEDPLFPASNESLQFSRRPDRHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDL-KEAPGNLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGL 295 HSP 2 Score: 166.007 bits (419), Expect = 1.282e-43 Identity = 94/272 (34.56%), Postives = 150/272 (55.15%), Query Frame = 1
Query: 1069 PVQREIKVDTGLIEGHAYSVTNVKEV----CSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI------NTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTIL-VNEKDPGKLCETIICITM---LEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESV 1818
P E + GLI GHAYS+T V + + +G+I ++R+RNPWGNE EW G +SD+SPEW I E ++ + L F+ DGEFWMSFQDF+ +F +EIC ++ D N+G ++W+ G+W+ + + D F NPQY T++ +E+D C I+ + + M + ++I+ + D N FF+ SS + F++ +C+R +LPPG+Y+I+PST E +F++R+ E + +ME V
Sbjct: 284 PNVTEAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMECV 555
BLAST of Calpain vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0340532) HSP 1 Score: 202.601 bits (514), Expect = 5.245e-55 Identity = 103/214 (48.13%), Postives = 140/214 (65.42%), Query Frame = 1
Query: 196 LSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPI----NHSQVQLAQKL 825
L+ LF DP F SN+ L S +W RP EI +NPQF +G SRFDV QG LGDCWLLAA +L+ +SNL +VI +Q F+++Y G F FR W+ G W DV++DDRLPT NG+L+YMHS+ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + + L +A ++ ++ KA+ S+M C I N ++ + Q L
Sbjct: 98 LASGSLFEDPLFPASNESLQFSRRPDRHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDL-KEAPGNLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGL 310 HSP 2 Score: 166.392 bits (420), Expect = 1.021e-42 Identity = 94/273 (34.43%), Postives = 150/273 (54.95%), Query Frame = 1
Query: 1069 PVQREIKVDTGLIEGHAYSVTNVKEV----CSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI------NTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTIL-VNEKDPGKLCETIICITM---LEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
P E + GLI GHAYS+T V + + +G+I ++R+RNPWGNE EW G +SD+SPEW I E ++ + L F+ DGEFWMSFQDF+ +F +EIC ++ D N+G ++W+ G+W+ + + D F NPQY T++ +E+D C I+ + + M + ++I+ + D N FF+ SS + F++ +C+R +LPPG+Y+I+PST E +F++R+ E + +ME D
Sbjct: 299 PNVTEAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEEND 571
BLAST of Calpain vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0112869) HSP 1 Score: 202.216 bits (513), Expect = 5.862e-55 Identity = 103/214 (48.13%), Postives = 140/214 (65.42%), Query Frame = 1
Query: 196 LSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPI----NHSQVQLAQKL 825
L+ LF DP F SN+ L S +W RP EI +NPQF +G SRFDV QG LGDCWLLAA +L+ +SNL +VI +Q F+++Y G F FR W+ G W DV++DDRLPT NG+L+YMHS+ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + + L +A ++ ++ KA+ S+M C I N ++ + Q L
Sbjct: 83 LASGSLFEDPLFPASNESLQFSRRPDRHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDL-KEAPGNLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGL 295 HSP 2 Score: 166.392 bits (420), Expect = 1.005e-42 Identity = 94/273 (34.43%), Postives = 150/273 (54.95%), Query Frame = 1
Query: 1069 PVQREIKVDTGLIEGHAYSVTNVKEV----CSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI------NTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTIL-VNEKDPGKLCETIICITM---LEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
P E + GLI GHAYS+T V + + +G+I ++R+RNPWGNE EW G +SD+SPEW I E ++ + L F+ DGEFWMSFQDF+ +F +EIC ++ D N+G ++W+ G+W+ + + D F NPQY T++ +E+D C I+ + + M + ++I+ + D N FF+ SS + F++ +C+R +LPPG+Y+I+PST E +F++R+ E + +ME D
Sbjct: 284 PNVTEAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEEND 556
BLAST of Calpain vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0086529) HSP 1 Score: 202.216 bits (513), Expect = 5.862e-55 Identity = 103/214 (48.13%), Postives = 140/214 (65.42%), Query Frame = 1
Query: 196 LSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPI----NHSQVQLAQKL 825
L+ LF DP F SN+ L S +W RP EI +NPQF +G SRFDV QG LGDCWLLAA +L+ +SNL +VI +Q F+++Y G F FR W+ G W DV++DDRLPT NG+L+YMHS+ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + + L +A ++ ++ KA+ S+M C I N ++ + Q L
Sbjct: 83 LASGSLFEDPLFPASNESLQFSRRPDRHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDL-KEAPGNLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGL 295 HSP 2 Score: 166.392 bits (420), Expect = 1.005e-42 Identity = 94/273 (34.43%), Postives = 150/273 (54.95%), Query Frame = 1
Query: 1069 PVQREIKVDTGLIEGHAYSVTNVKEV----CSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI------NTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTIL-VNEKDPGKLCETIICITM---LEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
P E + GLI GHAYS+T V + + +G+I ++R+RNPWGNE EW G +SD+SPEW I E ++ + L F+ DGEFWMSFQDF+ +F +EIC ++ D N+G ++W+ G+W+ + + D F NPQY T++ +E+D C I+ + + M + ++I+ + D N FF+ SS + F++ +C+R +LPPG+Y+I+PST E +F++R+ E + +ME D
Sbjct: 284 PNVTEAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEEND 556
BLAST of Calpain vs. Ensembl Fly
Match: CalpB (gene:FBgn0025866 transcript:FBtr0076343) HSP 1 Score: 201.06 bits (510), Expect = 3.080e-54 Identity = 100/209 (47.85%), Postives = 137/209 (65.55%), Query Frame = 1
Query: 160 SKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCC 786
SK F S + L+ +F DP F +N L S ++W RP +I +PQF +G SRFDV QG LGDCWLLAA +L+ DS L +VI DQ F ++Y G F F+ W+ G W +VV+DDRLPT NG+L+YMHS+ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + + + +A P ++ +M+KA+ S+M C
Sbjct: 242 SKGDFQSLRDSCLANGTMFEDPDFPATNASLMYSRRPDRYYEWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDI-KEAPPNLFSIMMKAAERGSMMGC 449 HSP 2 Score: 149.828 bits (377), Expect = 3.582e-37 Identity = 91/270 (33.70%), Postives = 146/270 (54.07%), Query Frame = 1
Query: 1081 EIKVDTGLIEGHAYSVTNV----KEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNKQ------WKTYNEFGKWSQNLGS-------DNFHLNPQYRFTI--LVNEKDPGKLCETIICITM---LEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
E + GLI GHAYS+T V + +G++ ++R+RNPWGN+ EW G +SD+SPEW I E ++ + LNF+ DGEFWMSFQDF+ +F +EIC ++ D T ++Q W+ G+W+ + + + F NPQY ++ +E D GK C I+ + V + + ++I+ + D FFK +S + F++ +C+R +LPPG+Y+I+PST E +F++R+ E +ME D
Sbjct: 459 EAETPQGLIRGHAYSITKVCLMDISTPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGK-CTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQGLNFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEEND 727
BLAST of Calpain vs. Ensembl Zebrafish
Match: capn3b (calpain 3b [Source:ZFIN;Acc:ZDB-GENE-041001-149]) HSP 1 Score: 196.823 bits (499), Expect = 1.985e-53 Identity = 101/219 (46.12%), Postives = 139/219 (63.47%), Query Frame = 1
Query: 166 KWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQK 822
K F + + K LF DP F + L+ S K +WKRP+EI KNPQFI G +R D+ QG LGDCWLLAAI L+L+ ++ +V+ DQ F ++Y G F F+ WR G W DVV+DDRLPT KLV+ S +EFWSAL+EKA+AKL GSY+ L GG S +AME TGG+ + + + ++A ++++M KA S+M C I+ + V AQ+
Sbjct: 59 KDFQELRDKYVRKKVLFEDPLFPAQDSSLFYSEKFPLKLEWKRPMEICKNPQFILGGANRTDICQGDLGDCWLLAAIACLTLNDVILKRVVPHDQSFTENYAGIFHFQFWRYGEWVDVVIDDRLPTYKNKLVFTRSGQNNEFWSALMEKAYAKLHGSYEALKGGNSLEAMEDFTGGVTEFYEI-TEAPKELYNIMRKALKRGSLMGCAID-TLVPTAQR 275 HSP 2 Score: 113.62 bits (283), Expect = 1.150e-25 Identity = 80/259 (30.89%), Postives = 130/259 (50.19%), Query Frame = 1
Query: 1066 TPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQ---INLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFI----YSIFKIEDARYLN--AQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRI 1785
P + K +GL+ GHAYSVT V++ +G+ I LVR+R+PWG V A N +W + + E+ L GEFWM F++F F+ LEIC +T D ++ +W G+W + + D + NPQ+R +L E D + T++ M +G V + + ++I+++ ++ N QL +FF N+S + + +++ + R L PG YVIIPST E++FLLR+
Sbjct: 270 VPTAQRTKTASGLVRGHAYSVTGVEQGKRKDGKDLMIRLVRVRDPWG--VAPPPACKSN--DWVELAKTEQDKQRLQPGEQGEFWMCFEEFQKNFTKLEICNLTPDTLEDDQMFKWNVTVHEGRWVKGCSAGGCRNFPDTYWTNPQFRLVLL--EADAKEKTCTVVVALMQKGRRVERCSGATLHNIGFAIYEVCSNKHGNQQQQLSKDFFLYNASTARSKSYVNIREVTERFCLKPGEYVIIPSTFDPHKESEFLLRL 522
BLAST of Calpain vs. Ensembl Zebrafish
Match: capn3b (calpain 3b [Source:ZFIN;Acc:ZDB-GENE-041001-149]) HSP 1 Score: 196.823 bits (499), Expect = 2.230e-53 Identity = 100/217 (46.08%), Postives = 138/217 (63.59%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQK 822
F + + K LF DP F + L+ S K +WKRP+EI KNPQFI G +R D+ QG LGDCWLLAAI L+L+ ++ +V+ DQ F ++Y G F F+ WR G W DVV+DDRLPT KLV+ S +EFWSAL+EKA+AKL GSY+ L GG S +AME TGG+ + + + ++A ++++M KA S+M C I+ + V AQ+
Sbjct: 52 FQELRDKYVRKKVLFEDPLFPAQDSSLFYSEKFPLKLEWKRPMEICKNPQFILGGANRTDICQGDLGDCWLLAAIACLTLNDVILKRVVPHDQSFTENYAGIFHFQFWRYGEWVDVVIDDRLPTYKNKLVFTRSGQNNEFWSALMEKAYAKLHGSYEALKGGNSLEAMEDFTGGVTEFYEI-TEAPKELYNIMRKALKRGSLMGCAID-TLVPTAQR 266 HSP 2 Score: 115.161 bits (287), Expect = 4.218e-26 Identity = 82/271 (30.26%), Postives = 133/271 (49.08%), Query Frame = 1
Query: 1066 TPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQ---INLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFI----YSIFKIEDARYLN--AQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
P + K +GL+ GHAYSVT V++ +G+ I LVR+R+PWG V A N +W + + E+ L GEFWM F++F F+ LEIC +T D ++ +W G+W + + D + NPQ+R +L E D + T++ M +G V + + ++I+++ N QL +FF N+S + + +++ + R L PG YVIIPST E++FLLR+ E + + +D
Sbjct: 261 VPTAQRTKTASGLVRGHAYSVTGVEQGKRKDGKDLMIRLVRVRDPWG--VAPPPACKSN--DWVELAKTEQDKQRLQPGEQGEFWMCFEEFQKNFTKLEICNLTPDTLEDDQMFKWNVTVHEGRWVKGCSAGGCRNFPDTYWTNPQFRLVLL--EADAKEKTCTVVVALMQKGRRVERCSGATLHNIGFAIYEVPKEMKGNQQQQLSKDFFLYNASTARSKSYVNIREVTERFCLKPGEYVIIPSTFDPHKESEFLLRVFSENRSTSKVID 525
BLAST of Calpain vs. Ensembl Zebrafish
Match: capn3b (calpain 3b [Source:ZFIN;Acc:ZDB-GENE-041001-149]) HSP 1 Score: 196.823 bits (499), Expect = 2.232e-53 Identity = 100/217 (46.08%), Postives = 138/217 (63.59%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQK 822
F + + K LF DP F + L+ S K +WKRP+EI KNPQFI G +R D+ QG LGDCWLLAAI L+L+ ++ +V+ DQ F ++Y G F F+ WR G W DVV+DDRLPT KLV+ S +EFWSAL+EKA+AKL GSY+ L GG S +AME TGG+ + + + ++A ++++M KA S+M C I+ + V AQ+
Sbjct: 52 FQELRDKYVRKKVLFEDPLFPAQDSSLFYSEKFPLKLEWKRPMEICKNPQFILGGANRTDICQGDLGDCWLLAAIACLTLNDVILKRVVPHDQSFTENYAGIFHFQFWRYGEWVDVVIDDRLPTYKNKLVFTRSGQNNEFWSALMEKAYAKLHGSYEALKGGNSLEAMEDFTGGVTEFYEI-TEAPKELYNIMRKALKRGSLMGCAID-TLVPTAQR 266 HSP 2 Score: 117.087 bits (292), Expect = 1.018e-26 Identity = 83/267 (31.09%), Postives = 133/267 (49.81%), Query Frame = 1
Query: 1066 TPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQ---INLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFIYSI-FKIEDAR-YLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
P + K +GL+ GHAYSVT V++ +G+ I LVR+R+PWG V A N +W + + E+ L GEFWM F++F F+ LEIC +T D ++ +W G+W + + D + NPQ+R +L E D + T++ M +G V + +++I F I + + QL +FF N+S + + +++ + R L PG YVIIPST E++FLLR+ E + + +D
Sbjct: 261 VPTAQRTKTASGLVRGHAYSVTGVEQGKRKDGKDLMIRLVRVRDPWG--VAPPPACKSN--DWVELAKTEQDKQRLQPGEQGEFWMCFEEFQKNFTKLEICNLTPDTLEDDQMFKWNVTVHEGRWVKGCSAGGCRNFPDTYWTNPQFRLVLL--EADAKEKTCTVVVALMQKGRRVERCSGATLHNIGFAIYEMKGNQQQQLSKDFFLYNASTARSKSYVNIREVTERFCLKPGEYVIIPSTFDPHKESEFLLRVFSENRSTSKVID 521
BLAST of Calpain vs. Ensembl Zebrafish
Match: capn8 (calpain 8 [Source:ZFIN;Acc:ZDB-GENE-050522-279]) HSP 1 Score: 194.897 bits (494), Expect = 7.024e-53 Identity = 100/218 (45.87%), Postives = 141/218 (64.68%), Query Frame = 1
Query: 163 KKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKF-------QWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
K+ + S K+ L+K LF DP F +D L + L ++ QWKRP E+ NP+FI DG +R D+ QG LGDCWLLAAI SL+LD ++ +V+ Q F + Y G F F+ W+ G W DVV+DDRLPT +GKL+++HS+ EFWSALLEKA+AK+ GSY+ L GG + + E TGGI + + L SKA P ++ ++ KA + S++ C I+
Sbjct: 29 KQDYQSLKQECLAKRALFCDPTFPAESDSL--GYNELGRYSFKTKGVQWKRPKELCSNPEFIMDGANRTDICQGALGDCWLLAAIASLTLDKEILERVVPSGQSFTEDYAGIFHFQFWQYGEWVDVVIDDRLPTRDGKLLFVHSAEGSEFWSALLEKAYAKVNGSYEALTGGSTTEGFEDFTGGITENYEL-SKAPPNLFKLVQKALALGSLLGCSID 243 HSP 2 Score: 126.331 bits (316), Expect = 1.132e-29 Identity = 88/252 (34.92%), Postives = 132/252 (52.38%), Query Frame = 1
Query: 1102 LIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEF-GKW---SQNLGSDN----FHLNPQYRFTILVNEKDPG---KLCETIICITMLEG---SLVNMPDSSFIYSIFKIED--ARYLNAQLDSE-FFKTNSSYLKYEFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPE 1800
L++GHAYSVT +EV + LVRIRNPWG EVEW GA+SDNS EW+++ E+ L+ + DGEFWM++ DFI FS LEIC +T D + + +W +Y++F G W S G N F NPQ+ + + DP C ++ + +G ++ ++I+K+ D N L + + S F++ + R +LPPG Y+I+PST + F+LR+ E E
Sbjct: 258 LVKGHAYSVTAAEEVHFRGSLVQLVRIRNPWG-EVEWTGAWSDNSKEWDSVPPEEKAKLDYS-AEDGEFWMAYSDFIQQFSKLEICNLTPDTLSSEEVNRW-SYSQFEGNWRVGSTAGGCRNNPATFCSNPQFMIKLEEIDDDPHDGVNGCTFLVGLMQKDGRKDKRFGRDLNTIGFAIYKVPDELKGRSNIHLGPDVLLRQRSVAGSNTFINLREVSERFKLPPGEYIIVPSTFEPHRKGSFILRVFAEKE 506
BLAST of Calpain vs. Ensembl Zebrafish
Match: capn8 (calpain 8 [Source:ZFIN;Acc:ZDB-GENE-050522-279]) HSP 1 Score: 194.897 bits (494), Expect = 7.222e-53 Identity = 100/218 (45.87%), Postives = 141/218 (64.68%), Query Frame = 1
Query: 163 KKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKF-------QWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
K+ + S K+ L+K LF DP F +D L + L ++ QWKRP E+ NP+FI DG +R D+ QG LGDCWLLAAI SL+LD ++ +V+ Q F + Y G F F+ W+ G W DVV+DDRLPT +GKL+++HS+ EFWSALLEKA+AK+ GSY+ L GG + + E TGGI + + L SKA P ++ ++ KA + S++ C I+
Sbjct: 29 KQDYQSLKQECLAKRALFCDPTFPAESDSL--GYNELGRYSFKTKGVQWKRPKELCSNPEFIMDGANRTDICQGALGDCWLLAAIASLTLDKEILERVVPSGQSFTEDYAGIFHFQFWQYGEWVDVVIDDRLPTRDGKLLFVHSAEGSEFWSALLEKAYAKVNGSYEALTGGSTTEGFEDFTGGITENYEL-SKAPPNLFKLVQKALALGSLLGCSID 243 HSP 2 Score: 126.716 bits (317), Expect = 7.209e-30 Identity = 86/249 (34.54%), Postives = 132/249 (53.01%), Query Frame = 1
Query: 1102 LIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEF-GKW---SQNLGSDN----FHLNPQYRFTILVNEKDPG---KLCETIICITMLEG---SLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKYEFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPE 1800
L++GHAYSVT +EV + LVRIRNPWG EVEW GA+SDNS EW+++ E+ L+ + DGEFWM++ DFI FS LEIC +T D + + +W +Y++F G W S G N F NPQ+ + + DP C ++ + +G ++ ++I+K++ ++ D + S F++ + R +LPPG Y+I+PST + F+LR+ E E
Sbjct: 258 LVKGHAYSVTAAEEVHFRGSLVQLVRIRNPWG-EVEWTGAWSDNSKEWDSVPPEEKAKLDYS-AEDGEFWMAYSDFIQQFSKLEICNLTPDTLSSEEVNRW-SYSQFEGNWRVGSTAGGCRNNPATFCSNPQFMIKLEEIDDDPHDGVNGCTFLVGLMQKDGRKDKRFGRDLNTIGFAIYKLKGRSNIHLGPDV-LLRQRSVAGSNTFINLREVSERFKLPPGEYIIVPSTFEPHRKGSFILRVFAEKE 502
BLAST of Calpain vs. Ensembl Xenopus
Match: CAPN3 (calpain 3 [Source:NCBI gene;Acc:100497532]) HSP 1 Score: 323.553 bits (828), Expect = 3.662e-99 Identity = 212/594 (35.69%), Postives = 312/594 (52.53%), Query Frame = 1
Query: 139 VSKNL---DDSKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGT--PVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQ-INLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSD-INTGNKQ-WKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK---LCETIICITMLEGSLVNMPDSSFI---YSIFKIEDARYLNAQ-LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPI 1848
+S+NL +K F +K L +N F D F P L+ S KF+WKRP EI +NPQFI G +R D+ QG LGDCW LAAI L+L+ ++ +VI DQ F +Y G F F+ WR G W DV++DD LPT N +LV+ SS +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + + L K +P+ ++++M KA S++ C I+ + G F P P N + ++ +K S G TN + +P +P T PVQ E ++ GL++GHAYSVT V+E L+G+ I LVR+RNPWG +VEW GA+ DN+ EW +++ E+ L+ DGEFWMS+ DF+ F+ EIC +T D +++ + Q W G+W + + D + NPQYR +L + DP +C ++ + ++ ++I+++ + N Q L +FF N+S K + +++ +C R RLPP YVIIPST E +F+LR+ E E V+ + PI
Sbjct: 38 ISRNLPIIGGREKTFEELRKKCLQRNFHFQDREFPPDESSLFYSQSFPIKFEWKRPPEICENPQFILGGANRTDICQGDLGDCWFLAAIACLTLNEKVLFRVIPPDQNFTDNYAGIFHFQFWRYGDWVDVIIDDYLPTYNNELVFTKSSQRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEL--KEAPKDMYNIMKKAFERGSLIGCSIDD---KYGSSCG-FAP-PSNMEDAI-------------------------------MKMIESVGDTRPDTNGR----------GQPPKPVATLVPVQLETRMANGLVKGHAYSVTGVEET-KLKGKPIKLVRLRNPWG-QVEWNGAWGDNAKEWTMVDKSEKTRLQHQVQEDGEFWMSYDDFMKNFTKAEICNLTPDALDSDSLQVWTVSVNEGRWVRGCSAGGCRNYPDTYWTNPQYRLKLLEEDDDPADNEVVCSFVVALMQKNRRKDRKAGANLFTIGFAIYEVPKEMHGNNQHLQKDFFLYNASKAKCKSYINMREVCQRFRLPPSEYVIIPSTYEPHQEGEFILRVFSEKRNISEEVENKIEAEKPI 581
BLAST of Calpain vs. Ensembl Xenopus
Match: CAPN3 (calpain 3 [Source:NCBI gene;Acc:100497532]) HSP 1 Score: 317.775 bits (813), Expect = 4.407e-97 Identity = 208/585 (35.56%), Postives = 305/585 (52.14%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCPSNDLL-----YCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGT--PVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQ-INLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSD-INTGNKQ-WKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK---LCETIICITMLEGSLVNMPDSSFI---YSIFKIEDARYLNAQ-LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPI 1848
F + K + K +LF DP F P+ L S L WKRP EI +NPQFI G +R D+ QG LGDCW LAAI L+L+ ++ +VI DQ F +Y G F F+ WR G W DV++DD LPT N +LV+ SS +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + + L K +P+ ++++M KA S++ C I+ + G F P P N + ++ +K S G TN + +P +P T PVQ E ++ GL++GHAYSVT V+E L+G+ I LVR+RNPWG +VEW GA+ DN+ EW +++ E+ L+ DGEFWMS+ DF+ F+ EIC +T D +++ + Q W G+W + + D + NPQYR +L + DP +C ++ + ++ ++I+++ + N Q L +FF N+S K + +++ +C R RLPP YVIIPST E +F+LR+ E E V+ + PI
Sbjct: 37 FATLKSEFVGKKKLFEDPIFLPAVQSLGQKEFAQKSTKLKNIIWKRPKEICENPQFILGGANRTDICQGDLGDCWFLAAIACLTLNEKVLFRVIPPDQNFTDNYAGIFHFQFWRYGDWVDVIIDDYLPTYNNELVFTKSSQRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEL--KEAPKDMYNIMKKAFERGSLIGCSIDD---KYGSSCG-FAP-PSNMEDAI-------------------------------MKMIESVGDTRPDTNGRG----------QPPKPVATLVPVQLETRMANGLVKGHAYSVTGVEET-KLKGKPIKLVRLRNPWG-QVEWNGAWGDNAKEWTMVDKSEKTRLQHQVQEDGEFWMSYDDFMKNFTKAEICNLTPDALDSDSLQVWTVSVNEGRWVRGCSAGGCRNYPDTYWTNPQYRLKLLEEDDDPADNEVVCSFVVALMQKNRRKDRKAGANLFTIGFAIYEVPKEMHGNNQHLQKDFFLYNASKAKCKSYINMREVCQRFRLPPSEYVIIPSTYEPHQEGEFILRVFSEKRNISEEVENKIEAEKPI 571
BLAST of Calpain vs. Ensembl Xenopus
Match: CAPN3 (calpain 3 [Source:NCBI gene;Acc:100497532]) HSP 1 Score: 302.753 bits (774), Expect = 1.763e-91 Identity = 210/591 (35.53%), Postives = 305/591 (51.61%), Query Frame = 1
Query: 139 VSKNL---DDSKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGT--PVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQ-INLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSD-INTGNKQ-WKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK---LCETIICITMLEGSLVNMPDSSFIYSI-FKIEDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPI 1848
+S+NL +K F +K L +N F D F P L+ S KF+WKRP EI +NPQFI G +R D+ QG LGDCW LAAI L+L+ ++ +VI DQ F +Y G F F+ WR G W DV++DD LPT N +LV+ SS +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + + L K +P+ ++++M KA S++ C I+ + G F P P N + ++ +K S G TN + +P +P T PVQ E ++ GL++GHAYSVT V+E L+G+ I LVR+RNPWG +VEW G W + E+ L+ DGEFWMS+ DF+ F+ EIC +T D +++ + Q W G+W + + D + NPQYR +L + DP +C ++ + M + + + +++I F I + N L +FF N+S K + +++ +C R RLPP YVIIPST E +F+LR+ E E V+ + PI
Sbjct: 47 ISRNLPIIGGREKTFEELRKKCLQRNFHFQDREFPPDESSLFYSQSFPIKFEWKRPPEICENPQFILGGANRTDICQGDLGDCWFLAAIACLTLNEKVLFRVIPPDQNFTDNYAGIFHFQFWRYGDWVDVIIDDYLPTYNNELVFTKSSQRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEL--KEAPKDMYNIMKKAFERGSLIGCSIDD---KYGSSCG-FAP-PSNMEDAI-------------------------------MKMIESVGDTRPDTNGR----------GQPPKPVATLVPVQLETRMANGLVKGHAYSVTGVEET-KLKGKPIKLVRLRNPWG-QVEWNG--------WAFVLS-EKTRLQHQVQEDGEFWMSYDDFMKNFTKAEICNLTPDALDSDSLQVWTVSVNEGRWVRGCSAGGCRNYPDTYWTNPQYRLKLLEEDDDPADNEVVCSFVVAL-MQKNRRKDRKAGANLFTIGFAIYEMHGNNQHLQKDFFLYNASKAKCKSYINMREVCQRFRLPPSEYVIIPSTYEPHQEGEFILRVFSEKRNISEEVENKIEAEKPI 577
BLAST of Calpain vs. Ensembl Xenopus
Match: CAPN3 (calpain 3 [Source:NCBI gene;Acc:100497532]) HSP 1 Score: 302.368 bits (773), Expect = 1.882e-91 Identity = 210/591 (35.53%), Postives = 305/591 (51.61%), Query Frame = 1
Query: 139 VSKNL---DDSKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGT--PVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQ-INLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSD-INTGNKQ-WKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK---LCETIICITMLEGSLVNMPDSSFIYSI-FKIEDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPI 1848
+S+NL +K F +K L +N F D F P L+ S KF+WKRP EI +NPQFI G +R D+ QG LGDCW LAAI L+L+ ++ +VI DQ F +Y G F F+ WR G W DV++DD LPT N +LV+ SS +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + + L K +P+ ++++M KA S++ C I+ + G F P P N + ++ +K S G TN + +P +P T PVQ E ++ GL++GHAYSVT V+E L+G+ I LVR+RNPWG +VEW G W + E+ L+ DGEFWMS+ DF+ F+ EIC +T D +++ + Q W G+W + + D + NPQYR +L + DP +C ++ + M + + + +++I F I + N L +FF N+S K + +++ +C R RLPP YVIIPST E +F+LR+ E E V+ + PI
Sbjct: 38 ISRNLPIIGGREKTFEELRKKCLQRNFHFQDREFPPDESSLFYSQSFPIKFEWKRPPEICENPQFILGGANRTDICQGDLGDCWFLAAIACLTLNEKVLFRVIPPDQNFTDNYAGIFHFQFWRYGDWVDVIIDDYLPTYNNELVFTKSSQRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEL--KEAPKDMYNIMKKAFERGSLIGCSIDD---KYGSSCG-FAP-PSNMEDAI-------------------------------MKMIESVGDTRPDTNGR----------GQPPKPVATLVPVQLETRMANGLVKGHAYSVTGVEE---LKGKPIKLVRLRNPWG-QVEWNG--------WAFVLS-EKTRLQHQVQEDGEFWMSYDDFMKNFTKAEICNLTPDALDSDSLQVWTVSVNEGRWVRGCSAGGCRNYPDTYWTNPQYRLKLLEEDDDPADNEVVCSFVVAL-MQKNRRKDRKAGANLFTIGFAIYEMHGNNQHLQKDFFLYNASKAKCKSYINMREVCQRFRLPPSEYVIIPSTYEPHQEGEFILRVFSEKRNISEEVENKIEAEKPI 566
BLAST of Calpain vs. Ensembl Xenopus
Match: CAPN3 (calpain 3 [Source:NCBI gene;Acc:100497532]) HSP 1 Score: 294.664 bits (753), Expect = 6.991e-89 Identity = 202/564 (35.82%), Postives = 292/564 (51.77%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCPSNDLL-----YCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGT--PVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQ-INLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSD-INTGNKQ-WKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK---LCETIICITMLEGSLVNMPDSSFIYSI-FKIEDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCE 1794
F + K + K +LF DP F P+ L S L WKRP EI +NPQFI G +R D+ QG LGDCW LAAI L+L+ ++ +VI DQ F +Y G F F+ WR G W DV++DD LPT N +LV+ SS +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + + L K +P+ ++++M KA S++ C I+ + G F P P N + ++ +K S G TN + +P +P T PVQ E ++ GL++GHAYSVT V+E L+G+ I LVR+RNPWG +VEW G W + E+ L+ DGEFWMS+ DF+ F+ EIC +T D +++ + Q W G+W + + D + NPQYR +L + DP +C ++ + M + + + +++I F I + N L +FF N+S K + +++ +C R RLPP YVIIPST E +F+LR+ E
Sbjct: 37 FATLKSEFVGKKKLFEDPIFLPAVQSLGQKEFAQKSTKLKNIIWKRPKEICENPQFILGGANRTDICQGDLGDCWFLAAIACLTLNEKVLFRVIPPDQNFTDNYAGIFHFQFWRYGDWVDVIIDDYLPTYNNELVFTKSSQRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEL--KEAPKDMYNIMKKAFERGSLIGCSIDD---KYGSSCG-FAP-PSNMEDAI-------------------------------MKMIESVGDTRPDTNGR----------GQPPKPVATLVPVQLETRMANGLVKGHAYSVTGVEE---LKGKPIKLVRLRNPWG-QVEWNG--------WAFVLS-EKTRLQHQVQEDGEFWMSYDDFMKNFTKAEICNLTPDALDSDSLQVWTVSVNEGRWVRGCSAGGCRNYPDTYWTNPQYRLKLLEEDDDPADNEVVCSFVVAL-MQKNRRKDRKAGANLFTIGFAIYEMHGNNQHLQKDFFLYNASKAKCKSYINMREVCQRFRLPPSEYVIIPSTYEPHQEGEFILRVFSE 538
BLAST of Calpain vs. Ensembl Mouse
Match: Capn1 (calpain 1 [Source:MGI Symbol;Acc:MGI:88263]) HSP 1 Score: 248.054 bits (632), Expect = 5.506e-72 Identity = 179/579 (30.92%), Postives = 265/579 (45.77%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCP-SNDL----LYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKI-VKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGN--KQWKTYNEFGKWSQNLGSD-------NFHLNPQYRFTILVNEKDPG-------KLCETIICITMLEGSLVNMPD---SSFIYSIFKIEDARYLNAQ---LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
+ + + L LF D F P S+ L L S +WKRP E+ NPQFI DG +R D+ QG LGDCWLLAAI SL+L+ ++++V+ Q F Y G F F+LW+ G W DVV+DD LPT++GKLV++HS+ +EFWSALLEKA+AK+ GSY+ L GG + +A E TGG+ + + L KA ++ +++KA S++ C IN I++ + AI+F + G+ YS T K + ++ + PWG V + PW SD+S EWN ++ ER+ L + DGEFWMSF+DFI F+ LEIC +T D + W T G W + + F +NPQ F I + E D C ++ + + ++++++ R L Q L +FF N+S + E F++ + +R RLPPG Y+++PST E DFLLR E + + +D
Sbjct: 42 YETLRARCLQSGVLFQDEAFPPVSHSLGFKELGPHSSKTYGIKWKRPTELMSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQDGYAGIFHFQLWQFGEWVDVVIDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDL-QKAPSDLYQIILKALERGSLLGCSIN------------------------ISDIRDLEAITF---------------------KNLVRGHAYSVTGAKQVTYQGQRVNLIRMRNPWGE-----------------------------------VEWKGPW----------SDSSYEWNKVDPYEREQLRVKM-EDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQ--FKIRLEEVDDADDYDNRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQV--PRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELD 524
BLAST of Calpain vs. Ensembl Mouse
Match: Capn1 (calpain 1 [Source:MGI Symbol;Acc:MGI:88263]) HSP 1 Score: 248.054 bits (632), Expect = 5.506e-72 Identity = 179/579 (30.92%), Postives = 265/579 (45.77%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCP-SNDL----LYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKI-VKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGN--KQWKTYNEFGKWSQNLGSD-------NFHLNPQYRFTILVNEKDPG-------KLCETIICITMLEGSLVNMPD---SSFIYSIFKIEDARYLNAQ---LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
+ + + L LF D F P S+ L L S +WKRP E+ NPQFI DG +R D+ QG LGDCWLLAAI SL+L+ ++++V+ Q F Y G F F+LW+ G W DVV+DD LPT++GKLV++HS+ +EFWSALLEKA+AK+ GSY+ L GG + +A E TGG+ + + L KA ++ +++KA S++ C IN I++ + AI+F + G+ YS T K + ++ + PWG V + PW SD+S EWN ++ ER+ L + DGEFWMSF+DFI F+ LEIC +T D + W T G W + + F +NPQ F I + E D C ++ + + ++++++ R L Q L +FF N+S + E F++ + +R RLPPG Y+++PST E DFLLR E + + +D
Sbjct: 42 YETLRARCLQSGVLFQDEAFPPVSHSLGFKELGPHSSKTYGIKWKRPTELMSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQDGYAGIFHFQLWQFGEWVDVVIDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDL-QKAPSDLYQIILKALERGSLLGCSIN------------------------ISDIRDLEAITF---------------------KNLVRGHAYSVTGAKQVTYQGQRVNLIRMRNPWGE-----------------------------------VEWKGPW----------SDSSYEWNKVDPYEREQLRVKM-EDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQ--FKIRLEEVDDADDYDNRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQV--PRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELD 524
BLAST of Calpain vs. Ensembl Mouse
Match: Capn1 (calpain 1 [Source:MGI Symbol;Acc:MGI:88263]) HSP 1 Score: 244.588 bits (623), Expect = 8.458e-71 Identity = 177/579 (30.57%), Postives = 264/579 (45.60%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCP-SNDL----LYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKI-VKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGN--KQWKTYNEFGKWSQNLGSD-------NFHLNPQYRFTILVNEKDPG-------KLCETIICITMLEGSLVNMPD---SSFIYSIFKIEDARYLNAQ---LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
+ + + L LF D F P S+ L L S +WKRP E+ NPQFI DG +R D+ QG LGDCWLLAAI SL+L+ ++++V+ Q F Y G F F+LW+ G W DVV+DD LPT++GKLV++HS+ +EFWSALLEKA+AK+ GSY+ L GG + +A E TGG+ + + L KA ++ +++KA S++ C IN I++ + AI+F + G+ YS T K + ++ + PWG V + PW + +S EWN ++ ER+ L + DGEFWMSF+DFI F+ LEIC +T D + W T G W + + F +NPQ F I + E D C ++ + + ++++++ R L Q L +FF N+S + E F++ + +R RLPPG Y+++PST E DFLLR E + + +D
Sbjct: 42 YETLRARCLQSGVLFQDEAFPPVSHSLGFKELGPHSSKTYGIKWKRPTELMSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQDGYAGIFHFQLWQFGEWVDVVIDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDL-QKAPSDLYQIILKALERGSLLGCSIN------------------------ISDIRDLEAITF---------------------KNLVRGHAYSVTGAKQVTYQGQRVNLIRMRNPWGE-----------------------------------VEWKGPWSD----------SSYEWNKVDPYEREQLRVKM-EDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQ--FKIRLEEVDDADDYDNRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQV--PRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELD 524
BLAST of Calpain vs. Ensembl Mouse
Match: Capn8 (calpain 8 [Source:MGI Symbol;Acc:MGI:2181366]) HSP 1 Score: 184.496 bits (467), Expect = 1.242e-51 Identity = 101/222 (45.50%), Postives = 138/222 (62.16%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFF--CPS----NDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQ 819
F + +K L+ LF DP F CPS DL S++ WKRP E+ NPQFI G +R D+ QG LGDCWLLAAI SL+L+ L+ +V+ DQ F K+Y G F F+ W+ G W +VV+DDRLPT+NG+L+++HS +EFWSALLEKA+AKL GSY+ L GG + + E TGGI + + L K ++ + KA S++ C I+ S A+
Sbjct: 32 FETLRKQCLNSGVLFKDPEFPACPSALGYRDLGPGSAE-TQGIIWKRPTELCSNPQFIVGGATRTDIRQGGLGDCWLLAAIASLTLNEKLLYRVVPRDQSFQKNYAGIFHFQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEEGNEFWSALLEKAYAKLNGSYEALAGGSTIEGFEDFTGGISEFYDL-RKPPGNLYYTIQKALRKGSLLGCSIDVSNAAEAE 251 HSP 2 Score: 98.2117 bits (243), Expect = 1.691e-21 Identity = 51/101 (50.50%), Postives = 66/101 (65.35%), Query Frame = 1
Query: 1072 VQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSD 1374
+ E L++GHAYSVT V+EV L+R+RNPWG EVEW GA+SD++PEWN I+ ++KG DGEFWMSF DF+ FS LEIC ++ D
Sbjct: 248 AEAEATTRQKLVKGHAYSVTGVEEVDFRGLPEKLIRLRNPWG-EVEWTGAWSDSAPEWNYIDP-QKKGELDKRAEDGEFWMSFSDFLKQFSRLEICNLSPD 346
BLAST of Calpain vs. Ensembl Mouse
Match: Capn3 (calpain 3 [Source:MGI Symbol;Acc:MGI:107437]) HSP 1 Score: 187.963 bits (476), Expect = 2.928e-50 Identity = 98/211 (46.45%), Postives = 133/211 (63.03%), Query Frame = 1
Query: 163 KKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
+K F ++ L K L+ DP F P L+ S +F WKRP EI +NP+FI G +R D+ QG LGDCW LAAI L+L+ L+ +VI DQ F ++Y G F F+ WR G W DVV+DD LPT N +LV+ S++ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + F + A ++ +M KA S+M C I+
Sbjct: 58 EKTFEQLRRKCLEKKVLYLDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEI-KDAPSDMYKIMRKAIERGSLMGCSID 267 HSP 2 Score: 146.362 bits (368), Expect = 3.750e-36 Identity = 90/280 (32.14%), Postives = 146/280 (52.14%), Query Frame = 1
Query: 1066 TPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDS--------SFIYSIFKIEDARYLNAQ-LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPI 1848
PVQ E ++ GL++GHAYSVT ++E ++ LVR+RNPWG +VEW G++SD +W+ +++ E+ L+ DGEFWMS+ DF+ +F+ LEIC +T+D +K W G+W + + D F NPQYR +L + D +IC ++ N + ++I+++ + N Q L +FF N+S + + +++ + R RLPP YVI+PST E +F+LR+ E E + T+ P+
Sbjct: 270 VPVQYETRMACGLVKGHAYSVTGLEEALFKGEKVKLVRLRNPWG-QVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDD--PEDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEAENTISVDRPV 546
BLAST of Calpain vs. UniProt/SwissProt
Match: sp|P43368|CAN3_PIG (Calpain-3 OS=Sus scrofa OX=9823 GN=CAPN3 PE=2 SV=2) HSP 1 Score: 308.916 bits (790), Expect = 8.514e-93 Identity = 203/589 (34.47%), Postives = 302/589 (51.27%), Query Frame = 1
Query: 163 KKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPIN------HSQVQLAQKLGLFIPN-PKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFI--------YSIFKIEDARYLNAQ-LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPI 1848
+K F K L K L+ DP F P L+ S +F WKRP EI +NP+FI G +R D+ QG LGDCW LAAI L+L+ L+ +VI DQ F ++Y G F F+ WR G W DVV+DD LPT N +LV+ S++ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + F + K +PR ++ +M KA S+M C I+ + K+G I +N D+S + + I C + +P +T + PVQ E ++ GL++GHAYSVT ++E ++ LVR+RNPWG +VEW G++SD+ +W+ +++ E+ L+ DGEFWMS+ DFI +F+ LEIC +T+D +K W G+W + + D F NPQYR +L + D +IC ++ N + ++I+++ + N Q L +FF N+S + +++ + R RLPP YVI+PST E +F+LR+ E E V+ T+ P+
Sbjct: 57 EKTFEQLHKKCLEKKVLYLDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNKRLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEI--KDAPRDMYKIMKKAIERGSLMGCSIDDGTNMTYGTSPSGLKMGDLIARMVRNMDESRL---RDSDLIPEGC------------SDDRPTRTIV-------------------------------PVQFETRMACGLVKGHAYSVTGLEEALFKGEKVKLVRLRNPWG-QVEWNGSWSDSWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFIYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDD--PDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSRTYINMREVSERFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPV 594
BLAST of Calpain vs. UniProt/SwissProt
Match: sp|Q92177|CAN3_CHICK (Calpain-3 OS=Gallus gallus OX=9031 GN=CAPN3 PE=2 SV=1) HSP 1 Score: 304.294 bits (778), Expect = 3.316e-91 Identity = 203/572 (35.49%), Postives = 294/572 (51.40%), Query Frame = 1
Query: 163 KKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK---LCETIICITMLEGSLVNMPDSSFIYSI-FKI----EDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
+K + K L +N L+ DP F P+ L+ S KF+WKRP EI +NP+FI G +R D+ QG LGDCW LAAI L+L+ L+ +VI DQ F ++Y G F F+ WR G W DV++DD LPT N +LV+ SS +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+I+ + + K +P+ I+ +M A S+M I+ + LG SD I E + + ++ + T +P T + Y E ++ GL++GHAYSVT V+E ++ LVR+RNPWG +VEW G +SD S EWN I+E E+ L+ DGEFW+S +DF+ +F+ LEIC +T D +K W G+W + + D F NPQYR +L + DP +C ++ + M + + +Y+I F I ++ L +FF N+S + + +++ I R RLPP YVIIPST E +F+LR+ E E V+
Sbjct: 52 EKTYEELHKKCLEENILYEDPDFPPNETSLFYSQKVPIKFEWKRPREICENPRFIIGGANRTDICQGELGDCWFLAAIACLTLNKKLLCRVIPHDQSFIQNYAGIFHFQFWRYGDWVDVIIDDCLPTYNNQLVFTKSSQRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVIEFYEI--KDAPKDIYKIMKHAIARGSLMASSIDDN-------LGFHYGAAPRSD---IGELIARMVKNLENAQMTYSTVDYQGTDERPAWTIMPMQY-------------------------------ETRMSCGLVKGHAYSVTAVEETTYKGEKMRLVRLRNPWG-QVEWNGPWSDKSEEWNFIDEEEKIRLQHKIAEDGEFWISLEDFMRHFTKLEICNLTPDTLEADKLQTWTVSVNEGRWVRGCSAGGCRNYPDTFWTNPQYRLKLLEEDDDPEDEEVICSFLVAL-MQKNRRKERKLGANLYTIGFAIYEVPKEMHGTKHHLQKDFFLYNASKARSKTYINMREISERFRLPPSEYVIIPSTYEPHQEGEFILRVFSEKRSLSEEVE 578
BLAST of Calpain vs. UniProt/SwissProt
Match: sp|Q9TTH8|CAN3_SHEEP (Calpain-3 OS=Ovis aries OX=9940 GN=CAPN3 PE=2 SV=1) HSP 1 Score: 296.975 bits (759), Expect = 2.898e-88 Identity = 198/589 (33.62%), Postives = 296/589 (50.25%), Query Frame = 1
Query: 163 KKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPIN------HSQVQLAQKLGLFIPN-PKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFI--------YSIFKIEDARYLNAQ-LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPI 1848
+K F + L K LF DP F P L+ S +F WKRP EI +NP+FI G +R D+ QG LGDCW LAAI L+L+ L+ +VI DQ F ++Y G F F+ WR G W DVV+DD LPT N +LV+ S++ +EFWSALLEKA+AKL GSY+ L G + + ME TG + + F + K +PR ++ +M KA S+M C I+ + K+G I +N D S + + I C + +P +T + Y E ++ GL++GHAYSVT ++E ++ LVR+RNPWG +VEW G++SD+ +W+ +++ E+ L+ DGEFWMS+ DFI +F+ LEIC +T+D +K W G+W + + D F NPQYR +L + D +IC ++ N + ++I+++ + N Q L +FF N+S + +++ + R RLPP YVI+PST E +F+LR+ E E V+ T+ P+
Sbjct: 58 EKTFEQLHRKCLEKKVLFVDPEFPPDETSLFYSQKFPIQFIWKRPPEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKAGNTTEGMEDFTGELTEFFEI--KDAPRDMYKIMKKAIERGSLMGCSIDDGTNMTYGTSPSGLKMGELIERMVRNMDNSRL---RDSDLIPEGC------------SDDRPTRTIVPVQY-------------------------------ETRMACGLVKGHAYSVTGLEEALYKGEKVKLVRLRNPWG-QVEWNGSWSDSWKDWSYVDKDEKARLQHQVTEDGEFWMSYDDFIYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDD--PDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSRTYINMREVSERFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPV 595
BLAST of Calpain vs. UniProt/SwissProt
Match: sp|Q9GLG7|CAN3_MACFA (Calpain-3 OS=Macaca fascicularis OX=9541 GN=CAPN3 PE=2 SV=1) HSP 1 Score: 268.47 bits (685), Expect = 9.397e-78 Identity = 186/581 (32.01%), Postives = 287/581 (49.40%), Query Frame = 1
Query: 163 KKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDS--------SFIYSIFKIEDARYLNAQ-LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPI 1848
+K F K L K L+ DP F P L+ S +F WKRP EI +NP+FI DG +R D+ QG LGDCW LAAI L+L+ L+ +VI DQ F ++Y G F F+ WR G W DVV+DD LPT N +LV+ S++ +EFWSALLEKA Y L G S++A++ G + + +++ S M IM ++ +G I + N + + + + + ++ D L+ ++ ++ V+ P PVQ E ++ GL+ GHAYSVT + EV ++ LVR+RNPWG +VEW G++SD +W+ +++ E+ L+ DGEFWMS++DFI +F+ LEIC +T+D +K W G+W + + D F NPQYR +L + D +IC ++ N + ++I+++ + N Q L +FF N+S + + +++ + R RLPP YVI+PST E +F+LR+ E E V+ T+ P+
Sbjct: 58 EKTFEQLHKKCLEKKVLYVDPEFPPDETSLFYSQKFPIQFIWKRPPEICENPRFIIDGANRTDICQGDLGDCWFLAAIACLTLNQRLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKA-------YAKLHG--SYEALKG--GNTTEAMEDFTGGVTEFFEIRDAPSDMHKIM-----KKAIERGSLMGCSIDDGTN-----------------MTYGTSPSGLNMGELIARMVRN--MDNSLFRDSD-----LDPRASVERPTRTI-VPVQYETRMACGLVRGHAYSVTGLDEVLFKGEKVKLVRLRNPWG-QVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDD--PDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNRQHLQKDFFLYNASRARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPV 594
BLAST of Calpain vs. UniProt/SwissProt
Match: sp|P97571|CAN1_RAT (Calpain-1 catalytic subunit OS=Rattus norvegicus OX=10116 GN=Capn1 PE=1 SV=1) HSP 1 Score: 251.136 bits (640), Expect = 3.287e-72 Identity = 181/571 (31.70%), Postives = 265/571 (46.41%), Query Frame = 1
Query: 196 LSKNQLFHDPFFCP-SNDL----LYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKI-VKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGN--KQWKTYNEFGKWSQNLGSD-------NFHLNPQYRFTILVNEKDPG-------KLCETIICITMLEGSLVNMPD---SSFIYSIFKIEDARYLNAQ---LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
L LF D F P S+ L L +S +WKRP E+ NPQFI DG +R D+ QG LGDCWLLAAI SL+L+ ++++V+ Q F + Y G F F+LW+ G W DVVVDD LPT++GKLV++HS+ +EFWSALLEKA+AK+ GSY+ L GG + +A E TGG+ + + L KA ++ +++KA S++ C IN I++ + AI+F + G+ YS T+ K + ++ + PWG V + PW SDNS EWN ++ ER+ L + DGEFWMSF+DFI F+ LEIC +T D + W T G W + + F +NPQ F I + E D C ++ + + ++++++ R L Q L +FF N+S + E F++ + +R RLPPG Y+++PST E DFLLR E + + +D
Sbjct: 50 LQNGVLFQDDAFPPVSHSLGFKELGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQEGYAGIFHFQLWQFGEWVDVVVDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDL-QKAPSDLYQIILKALERGSLLGCSIN------------------------ISDIRDLEAITF---------------------KNLVRGHAYSVTDAKQVTYQGQRVNLIRMRNPWGE-----------------------------------VEWKGPW----------SDNSYEWNKVDPYEREQLRVKM-EDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQ--FKIRLEEVDDADDYDSRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQV--PRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELD 524
BLAST of Calpain vs. TrEMBL
Match: A0A310SH88 (Calpain-B OS=Eufriesea mexicana OX=516756 GN=WN48_06682 PE=3 SV=1) HSP 1 Score: 332.413 bits (851), Expect = 6.093e-100 Identity = 203/567 (35.80%), Postives = 292/567 (51.50%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEIC------YVTSDINTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFI---YSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
FN+ K+ LS LF DP F ++ LY S +WKRP+EI NPQ +G SRFDV QG LGDCWLLAA+ +L++D NL QV+ DQ F+++YVG F FR W+ G W DVV+DDRLPT G+L+Y+HS+ ++EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ +++++ + P ++++++KA S+M C I P+P +++ + I G+ YS T KH++I + PN+ P+ R +RNPWGNE EW G +SD SPEW I + E+ L L F+ DGEFWMSFQDF YF+ LEIC SD++ G K+W+ G+W + + + + F NPQYR T+ ++D K C I+ + + + ++I+ ++ L LD FFK N+S + F++ + R +LPPG Y I+PST E +FLLRI E + +ME D
Sbjct: 52 FNTLKRECLSTGTLFEDPEFPANDSSLYFSKRPDRYIEWKRPMEIADNPQLFVEGFSRFDVQQGELGDCWLLAAVANLTMDQNLFFQVVPEDQSFEENYVGIFHFRFWQYGRWVDVVIDDRLPTYRGELLYLHSAEVNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEMYQM-DETPPNLFNILLKAYERNSLMGCSIE--------------PDP-----NILEAETPQGLIR---------------------------GHAYSITRVKHVEI------QTPNQYGRIPLLR---------------------------------LRNPWGNEAEWNGPWSDQSPEWRFIPDHEKDELGLTFDMDGEFWMSFQDFTRYFTQLEICNLNPDSLTESDLSAGKKKWEMSVFEGEWVRGVTAGGCRNFLETFWHNPQYRITLEYPDEDDDK-CTVIVALMQKNRRAQRRMGADCLTIGFAIYHLDYPERLPKPLDVNFFKYNASVARSPAFINLREVTCRFKLPPGVYCIVPSTFDPNEEGEFLLRIFSENKNNMEEND 531
BLAST of Calpain vs. TrEMBL
Match: E2ADL6 (Calpain-A OS=Camponotus floridanus OX=104421 GN=EAG_10351 PE=3 SV=1) HSP 1 Score: 330.487 bits (846), Expect = 2.087e-99 Identity = 202/567 (35.63%), Postives = 290/567 (51.15%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEIC------YVTSDINTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFI---YSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
FN+ ++ L+ +LF DP F + LY S +WKRP+EI NPQ +G SRFDV QG LGDCWLLAA+ +L++ SNL QV+ DQ F+++Y G F FR W+ G W DVV+DDRLPT G+LVY+HS+ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ +++++ + P ++++++KA S++ C I P+P +++ + I G+ YS T+ KH++I TP Q G I L+R+RNPWGNE EW G +SD SPEW I + E++ L L F+ DGEFWMSF DF +F+ LEIC DI+ G K+W+ G+W + + + + F NPQYR T+ + D K C I+ + + + ++I+ +E L LD FFK N+S + F++ + R +LPPG Y I+PST E +FLLRI E + +ME D
Sbjct: 41 FNTLRRECLATGRLFEDPEFLADDSSLYFSRRPDRYIEWKRPMEIVDNPQLFVEGFSRFDVQQGELGDCWLLAAVANLTMHSNLFFQVVPEDQSFEENYAGIFHFRFWQYGRWVDVVIDDRLPTCRGELVYLHSAESNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEMYQM-DQTPPNLFNILLKAFERNSLLGCSIE--------------PDP-----NILEAETPQGLIR---------------------------GHAYSITHVKHVEIQ-------------TPNQH--------------------------GTIPLLRLRNPWGNEAEWNGPWSDQSPEWRFIPDHEKEELGLTFDMDGEFWMSFHDFTRHFTQLEICNLNPDSLTADDIDAGKKRWEMSVFEGEWVRGVTAGGCRNYLETFCHNPQYRITLEYPDDDDDK-CTVIVALMQKNRRAQKRMGADCLTIGFAIYHLEYPERLPKPLDINFFKYNASVGRSPAFINLREVTCRFKLPPGVYCIVPSTFDPNEEGEFLLRIFSENKNNMEEND 520
BLAST of Calpain vs. TrEMBL
Match: A0A026WP62 (Calpain-B OS=Ooceraea biroi OX=2015173 GN=X777_00848 PE=3 SV=1) HSP 1 Score: 331.257 bits (848), Expect = 2.714e-99 Identity = 202/567 (35.63%), Postives = 291/567 (51.32%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSD------INTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFI---YSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
FN ++ L+ +LF DP F ++ LY S +WKRP+EI NPQF +G SRFDV QG LGDCWLLAA+ +L++ NL QV+ DQ FD++Y G F FR W+ G W DVV+DDRLPT G+L+Y+HS+ DEFWSALLEKA+AKL GSY+ L GG + +AME TGG+ +++++ + P ++++++KA S++ C I P+P +V+ + I G+ YS T KH++I + PN+ P L+R+RNPWGNE EW G +SD SPEW I + E++ L L F+ DGEFWMSF DF+ +F+ LEIC + D +N G K+W+ G+W + + + + F NPQYR T+ + D K C I+ + + + ++I+ +E+ L LD FFK N S + F++ + R +LPPG Y I+PST E +FLLRI E + +ME D
Sbjct: 77 FNVLRRECLASGRLFEDPEFPANDSSLYFSRRPDRYIEWKRPMEIADNPQFFVEGFSRFDVQQGELGDCWLLAAVANLTMHPNLFFQVVPEDQSFDENYAGIFHFRFWQYGRWVDVVIDDRLPTFRGELMYLHSAENDEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEMYQM-DQTPPNLFNILLKAFERNSLLGCSIE--------------PDP-----NVVEAETPQGLIR---------------------------GHAYSVTRVKHVEI------QTPNQIGTIP---------------------------------LLRLRNPWGNEAEWNGPWSDKSPEWRFIPDHEKEELGLTFDMDGEFWMSFHDFVRHFTQLEICNLNPDSLTIDELNAGKKRWEMSVFEGEWVRGVTAGGCRNYLETFWHNPQYRITLECPDDDDDK-CTVIVALMQKNRRAQKRMGADCLTIGFAIYHLENPERLPKPLDMSFFKYNPSIGRSPAFINLREVTCRFKLPPGVYCIVPSTFDPNEEGEFLLRIFSENKNNMEEND 556
BLAST of Calpain vs. TrEMBL
Match: E9IT45 (Uncharacterized protein (Fragment) OS=Solenopsis invicta OX=13686 GN=SINV_03488 PE=3 SV=1) HSP 1 Score: 329.331 bits (843), Expect = 3.452e-98 Identity = 205/580 (35.34%), Postives = 293/580 (50.52%), Query Frame = 1
Query: 133 FRVSKNLDDSKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEIC------YVTSDINTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFI---YSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
FR + D FN ++ L+ +LF DP F ++D LY S +W+RP+EI NPQ +G SRFDV QG LGDCWLLAA+ +L++ SNL Q++ DQ FD++Y G F FR W+ G W DVV+DDRLPT +GKL+Y+ S+ +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ +++++ + P ++++++KA S++ C I P+P +++ + I G+ YS T KH++I + PN P+ R +RNPWGNE EW G +SD SPEW I E E++ L L F+ DGEFWMSF DF+ +F+ LEIC T DI+ G K+W+ G+W + + + + F NPQYR T L N D C II + + + ++I+ +E L LD FFK N+S + F++ + R +LPPG Y I+PST E +FLLRI E + +ME D
Sbjct: 75 FRARGEVQD----FNKLRRECLATGRLFEDPEFPANDDSLYFSRRPDRYIEWRRPMEIADNPQLFVEGYSRFDVQQGELGDCWLLAAVANLTMHSNLFFQIVPEDQSFDENYAGIFHFRFWQYGRWVDVVIDDRLPTYHGKLMYLQSAETNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEMYQM-DETPPNLFNILLKAFERNSLLGCSIE--------------PDP-----NILEAETPQGLIR---------------------------GHAYSITCVKHVEI------QMPNRQGTIPLLR---------------------------------LRNPWGNEAEWNGPWSDKSPEWRFIPEHEKEELGLTFDMDGEFWMSFHDFVRHFTQLEICNLNPDSLTTDDISIGKKRWEMSVFEGEWVRGVTAGGCRNFLETFCHNPQYRIT-LENPDDDDDKCTVIIALMQKNRRAQRRMGADCLTIGFAIYHLEYPDRLPKPLDVNFFKYNASVGRSPSFINLREVTCRFKLPPGVYCIVPSTFDPNEEGEFLLRIFSENKNNMEEND 563
BLAST of Calpain vs. TrEMBL
Match: A0A452GPR9 (Calpain 3 OS=Gopherus agassizii OX=38772 GN=CAPN3 PE=3 SV=1) HSP 1 Score: 329.331 bits (843), Expect = 4.287e-98 Identity = 212/580 (36.55%), Postives = 304/580 (52.41%), Query Frame = 1
Query: 163 KKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPINHS---------QVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK---LCETIICITMLEGSLVNMPDSSFI---YSIFKIEDARYLNAQ-LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
+K F K L K L+ DP F P+ L+ S KF+WKRP EI +NP+FI G +R D+ QG LGDCW LAAI L+L+ L+ +VI DQ F K+Y G F F+ WR G W DVV+DD LPT N +LV+ SS +EFWSALLEKA+AKL GSY+ L GG + +AME TGG+ + + + K +P+ I+ +M A S+M C I+ + Q L + + + K+V + P+ S + F + T +P T + P+Q E ++ GL++GHAYSVT V+E G+I LVR+RNPWG +VEWKGA+SDNS EWN +++ E+ L+ DGEFWMSF DF+ YF+ LEIC +T D +K W G+W + + D F NPQYR +L + DP +C ++ + ++ + ++I+++ + N Q L +FF N+S + + +++ I R RLPP YVIIPST E +F+LR+ E E V+
Sbjct: 52 EKTFEELHKKCLEKKILYVDPDFPPNESSLFYSQKVPIKFEWKRPTEICENPRFIIGGANRTDICQGELGDCWFLAAIACLTLNEKLLCRVIPHDQTFIKNYAGIFHFQFWRYGNWVDVVIDDCLPTYNNQLVFTKSSQRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEI--KDAPKDIYKIMKHAKDRGSLMACSIDDNMGFSYGTAPQTDLGKLIAQMV-------KNVNSMPRTDSTLDF------------QGTDERPAWTIV-------------------------------PMQLETRMTCGLVKGHAYSVTAVEETSFKAGKIKLVRLRNPWG-QVEWKGAWSDNSDEWNFVDKAEKIRLQYRIAEDGEFWMSFDDFMRYFTKLEICNLTPDTLEADKLQTWTVSVNEGRWVRGCSAGGCRNYPDTFWTNPQYRLELLEEDDDPEDNEVICSFLVALMQKNRRKERKLGANLLTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKSYINMREISERFRLPPNEYVIIPSTYEPHQEGEFILRVFSEKRSLSEEVE 578
BLAST of Calpain vs. Ensembl Cavefish
Match: capn3b (calpain 3b [Source:ZFIN;Acc:ZDB-GENE-041001-149]) HSP 1 Score: 197.593 bits (501), Expect = 1.068e-53 Identity = 100/200 (50.00%), Postives = 129/200 (64.50%), Query Frame = 1
Query: 196 LSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
+ K LF DP F + LY + K WKRP EI KNPQFI G +R D+ QG LGDCWLLAAI L+L L+ +V+ DQ F ++Y G F F+ WR G W DVVVDDRLPT N LV+ S N +EFWSALLEKA+AKL G+Y+ L GG + +AME TGG+ + + + S A I+++M KA + S+M C I+
Sbjct: 74 VRKKVLFKDPLFPADDSSLYYNQKFPLKLVWKRPSEICKNPQFIVGGANRTDICQGDLGDCWLLAAIACLTLSETLLYRVVPADQSFSENYAGIFHFQFWRYGEWVDVVVDDRLPTSNNHLVFTRSGNKNEFWSALLEKAYAKLHGTYEALKGGNTLEAMEDFTGGVTEFYEI-SDAPREIYNIMKKALNRGSLMGCSID 272 HSP 2 Score: 115.161 bits (287), Expect = 3.830e-26 Identity = 82/259 (31.66%), Postives = 125/259 (48.26%), Query Frame = 1
Query: 1069 PVQREIKVDTGLIEGHAYSVTNVKEVCSLEG---QINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILV---NEKDPGKLCETIICITMLEGSLVNMPDSSFIYSI-FKIEDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCE 1794
P E K DTGL+ GHAYSVT V++ +G +I LVR+R+PWG S S +W+++ E+ L GEFWM F++F F+ LEIC +T D ++ +W G+W + + D F NPQYR + +E D G+ T++ M +G + +I F I + L +FF +S + + +++ + R L PG YVI+PST E+DF+LR+ E
Sbjct: 276 PSASETKTDTGLVRGHAYSVTAVEQGKQEDGSDTRIRLVRLRDPWG----VAPPPSRMSCDWSSLATSEQDQQRLKPVEAGEFWMGFEEFQQTFTKLEICNLTPDALQDDELLKWDVSVNEGRWVRGCSAGGCRNYPDTFWTNPQYRLRLTEEDDDEPDHGEKGCTVVVALMQKGRRRERCTGGTLLTIGFAIYEMHGNKQHLPKDFFLYTASKARCKSYINLREVTERFCLSPGEYVIVPSTFDPNNESDFILRVFSE 530
BLAST of Calpain vs. Ensembl Cavefish
Match: capn2a (calpain-2 catalytic subunit-like [Source:NCBI gene;Acc:107197131]) HSP 1 Score: 188.734 bits (478), Expect = 5.072e-51 Identity = 94/214 (43.93%), Postives = 140/214 (65.42%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFF--CPSN---DLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPIN 795
F + + L++ QLF DP F CP + + L S QWKRP E+ +P+FI G +R D+ QG LGDCWLLAAI SL+L+ +++++V+ DQ F +Y G F F+ W+ G W DVV+DDRLPT +G+L+++HS+ EFWSALLEKA+AKL G Y+ L GG + + E TGGI +++ L K++PR ++ ++ KA +++ C I+
Sbjct: 32 FETLRSECLTRGQLFSDPTFPACPESLGFNELGPRSSKTRGVQWKRPGELTSSPEFIVGGATRTDICQGGLGDCWLLAAIASLTLNEDVLSRVVPSDQGFGDNYAGIFHFQFWQYGEWVDVVIDDRLPTRDGELLFVHSATGSEFWSALLEKAYAKLNGCYEALSGGSTTEGFEDFTGGIAEMYEL--KSAPRNMFQIIQKALESGALLGCSID 243 HSP 2 Score: 132.494 bits (332), Expect = 6.715e-32 Identity = 91/267 (34.08%), Postives = 137/267 (51.31%), Query Frame = 1
Query: 1102 LIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGN--KQWKTYNEFGKW---SQNLGSDN----FHLNPQYRFTILVNEKDPGKL---CETIICITMLEGSLVNMPDSSFIYSIFKIEDARYLNAQ----LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPIN 1851
L++GHAYSVT EV + L+RIRNPWG +VEW GA+SDNS EWN+++ ER+ ++ + DGEFWMSF +F ++S LEIC +T D + K W G W S G N F +NPQY+ + + DP C +I + + + I + R + Q L E+F T++ + E F++ + +R +LPPG Y+I+PST DF +R+ E + M +D I +N ++
Sbjct: 258 LVKGHAYSVTGATEVNFRGRKEKLIRIRNPWG-QVEWTGAWSDNSSEWNSVDPSERENVKAD---DGEFWMSFTEFSRHYSRLEICTLTPDTLASDSVKHWSVCKFDGSWRRGSTAGGCRNNPYTFWMNPQYKIKLDEEDDDPDDNEVGCSIVIGLIQKNRRKLRKSGEDMHTIGYAIYEVRGFHGQKEVHLSKEYFLTHAQKARSETFINLREVSTRFKLPPGEYLIVPSTFEPHKNGDFCVRVFSEKQSEMH-LDTCRIMVNLMD 519
BLAST of Calpain vs. Ensembl Cavefish
Match: capn2a (calpain-2 catalytic subunit-like [Source:NCBI gene;Acc:107197131]) HSP 1 Score: 189.119 bits (479), Expect = 7.500e-51 Identity = 94/214 (43.93%), Postives = 140/214 (65.42%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFF--CPSN---DLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPIN 795
F + + L++ QLF DP F CP + + L S QWKRP E+ +P+FI G +R D+ QG LGDCWLLAAI SL+L+ +++++V+ DQ F +Y G F F+ W+ G W DVV+DDRLPT +G+L+++HS+ EFWSALLEKA+AKL G Y+ L GG + + E TGGI +++ L K++PR ++ ++ KA +++ C I+
Sbjct: 32 FETLRSECLTRGQLFSDPTFPACPESLGFNELGPRSSKTRGVQWKRPGELTSSPEFIVGGATRTDICQGGLGDCWLLAAIASLTLNEDVLSRVVPSDQGFGDNYAGIFHFQFWQYGEWVDVVIDDRLPTRDGELLFVHSATGSEFWSALLEKAYAKLNGCYEALSGGSTTEGFEDFTGGIAEMYEL--KSAPRNMFQIIQKALESGALLGCSID 243 HSP 2 Score: 132.109 bits (331), Expect = 1.035e-31 Identity = 92/260 (35.38%), Postives = 137/260 (52.69%), Query Frame = 1
Query: 1102 LIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGN--KQWKTYNEFGKW---SQNLGSDN----FHLNPQYRFTILVNEKDPGKL---CETIICITM-----LEGSLVNMPDSSFIYSIFKI--EDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
L++GHAYSVT EV + L+RIRNPWG +VEW GA+SDNS EWN+++ ER+ ++ + DGEFWMSF +F ++S LEIC +T D + K W G W S G N F +NPQY+ + + DP C +I + L S +M + Y+I+++ E L E+F T++ + E F++ + +R +LPPG Y+I+PST DF +R+ E + M+ D
Sbjct: 258 LVKGHAYSVTGATEVNFRGRKEKLIRIRNPWG-QVEWTGAWSDNSSEWNSVDPSERENVKAD---DGEFWMSFTEFSRHYSRLEICTLTPDTLASDSVKHWSVCKFDGSWRRGSTAGGCRNNPYTFWMNPQYKIKLDEEDDDPDDNEVGCSIVIGLIQKNRRKLRKSGEDM--HTIGYAIYEVPPEFHGQKEVHLSKEYFLTHAQKARSETFINLREVSTRFKLPPGEYLIVPSTFEPHKNGDFCVRVFSEKQSEMQICD 511
BLAST of Calpain vs. Ensembl Cavefish
Match: ENSAMXT00000014125.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000051.1:1440473:1447479:1 gene:ENSAMXG00000013724.2 transcript:ENSAMXT00000014125.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 178.718 bits (452), Expect = 8.714e-50 Identity = 95/215 (44.19%), Postives = 136/215 (63.26%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFFCPSNDLLYCS-----SDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHS 801
F + L QLF DP F + + L + S QW RP E+ +NPQFI DG +R DV QG LGDCWL+AAI SL+LD +++ +VI +Q F ++Y G F F+LW+ G W DVVVDDRLPT NG+L+++HS + +EFW+ALLEKA+AK+ SY++L GG + + E TGGI + ++L S A ++ +M +A +++ I S
Sbjct: 24 FEVLRSRCLRSGQLFCDPAFPAAPESLGFNELGPNSPKTAGVQWIRPGELCQNPQFIVDGATRTDVCQGSLGDCWLMAAIASLTLDRDVLARVIYPEQSFTENYAGIFHFQLWQYGQWVDVVVDDRLPTINGELLFVHSEDGNEFWTALLEKAYAKVNSSYESLKGGSAIEGFEDFTGGISERYQL-SNAPANLFKIMKQALRRGALLSTAIAVS 237 HSP 2 Score: 78.5666 bits (192), Expect = 3.165e-15 Identity = 40/97 (41.24%), Postives = 63/97 (64.95%), Query Frame = 1
Query: 1102 LIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNF-NNDGEFWMSFQDFITYFSSLEICYVTSDINTGN 1389
L++ HAYS+T +EV + L+R+RNPWG++ EW GA+SD S EW+ ++ + +LN+ +DGEFWMS+ DF +S +EIC + + + N
Sbjct: 247 LVKRHAYSITAAEEVQVGGRCVQLLRLRNPWGHK-EWSGAWSDESREWSKVSPEVKD--QLNYCADDGEFWMSYTDFKVEYSKVEICNLIPETPSNN 340
BLAST of Calpain vs. Ensembl Cavefish
Match: capn8 (calpain 8 [Source:ZFIN;Acc:ZDB-GENE-050522-279]) HSP 1 Score: 184.882 bits (468), Expect = 1.317e-49 Identity = 106/270 (39.26%), Postives = 157/270 (58.15%), Query Frame = 1
Query: 55 KMAKNEQSRTVSMESNSETVTFDNTEFRVSKNLDDSKKWFNSQKKVLLSKNQLFHDPFF-CPSNDLLYCSSDHLDKFQ-------WKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN-----HSQVQLAQKL 825
++AK Q + + SN + F+N ++ S ++ L + LF DP F S+ L Y + L ++ WKRP E+ NP+FI +G +R D+ QG LGDCWLLAAI SL+LD++++ +V+ Q F + Y G F F+LW+ G W DVV+DDRLPT+NG+L+++HS EFWSALLEKA+AKL SY+ L GG + + TGGI + + L KA P +++++ KA S++ C I+ S+ QKL
Sbjct: 7 ELAK-RQDKKEGLGSNDNAIPFNNQDYL-------------SLRRECLQRKTLFCDPTFPADSSSLGY---NQLGRYSSKTAGVVWKRPTELCSNPEFISNGATRMDIRQGALGDCWLLAAIASLTLDTDILARVVPPAQSFKEDYAGIFHFQLWQYGEWIDVVIDDRLPTKNGELLFVHSEEGSEFWSALLEKAYAKLNSSYEALSGGSTTEGFVDFTGGIAESYEL-RKAPPTLFNIIKKALSRGSMLGCSIDIANSYESEAMTRQKL 258 HSP 2 Score: 126.331 bits (316), Expect = 7.880e-30 Identity = 81/248 (32.66%), Postives = 129/248 (52.02%), Query Frame = 1
Query: 1102 LIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI--NTGNKQWKTYNEFGKW---SQNLGSDN----FHLNPQYRFTILVNEKDPGKL---CETIICITMLEGSL-----VNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKYEFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCE 1794
L++GHAYS+T +EV + LVR+RNPWG +VEW GA+SD+S EW+ + E+ L+ + +DGEFWM + DF+ +S +EIC +T D + G +W Y G W S G N F NPQ+ + + DP C ++ + +G ++ F +F+ ++ +L + + + + F++ +CSR +LPPG YVIIPST + +F+LR+ E
Sbjct: 258 LVKGHAYSLTAAEEVHVSGRPVQLVRLRNPWG-QVEWTGAWSDDSKEWDRVLPEEKAKLDYS-ADDGEFWMEYSDFLNQYSKIEICNLTPDTLSSEGVCRWSCYQFEGSWKVGSTAGGCRNNPATFCSNPQFVIKLEEVDDDPHDGVDGCSFLVGLMQRDGRKERQFGRDLNTVGFAIYMFRGQNNIHLGPDV---LLRQRAIAMSDTFINLREVCSRFKLPPGEYVIIPSTFEPHRKGNFILRVFAE 500
BLAST of Calpain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003998.1 (pep scaffold:Pmarinus_7.0:GL501031:16584:50555:1 gene:ENSPMAG00000003440.1 transcript:ENSPMAT00000003998.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 300.056 bits (767), Expect = 5.011e-92 Identity = 194/520 (37.31%), Postives = 276/520 (53.08%), Query Frame = 1
Query: 289 KRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQ-INLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSD---INTGNKQWKTYNEFGKWSQNLG-------SDNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDS-----SFIYSIFKIE-DARYLNAQLDSEFFKTNSSYLK-YEFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCE 1794
+ P EI NPQFI +G +R D+ QG LGDCWLLAAI SL+L L+ +V+ Q F Y G F F+ W+ G W DVV+DD LPT+N KL ++HS++ +EFWSALLEKA+AKL GSY+ L GG +AME TGG+ + + L KA P ++ +M K S+M C I+ S IN K + +F +S ++ +L S+ + + +++ + E ++ GL++GHAYS+T + +V EGQ + LVRIRNPWG EVEW GA+SDNS EW ++ ER+ LE DGEFWMSF DF FS +EIC ++ D +G++ W G+W + + F NPQ F + ++E+D + + C ++ NM + Y+I++I A L EFF NSS + + F++ + R RLPPG+YVI+PST EADFLLR+ CE
Sbjct: 1 RAPQEICYNPQFIVNGATRTDIKQGALGDCWLLAAIASLTLHPRLLARVVPEGQSFSDGYAGIFHFQFWQFGEWVDVVIDDLLPTQNNKLAFLHSTDRNEFWSALLEKAYAKLNGSYEALKGGSIAEAMEDFTGGVFEFYNL-KKAPPDLYRIMRKGLEKASMMGCSIDEGST-----------FKYRSTPRAINMSKMTA--TFRAWSSGI--------------RRFAERFLPSR-------LGHARLLASSGIEITSMFDLEARLPNGLVKGHAYSITGLDQVF-FEGQDVRLVRIRNPWG-EVEWNGAWSDNSWEWRVVDVSERRRLEQVRAEDGEFWMSFSDFKRNFSKMEICNLSPDTLRTQSGSRHWTVTAHEGRWLRGCTAGGCRNYAGTFWTNPQ--FHLRLHEEDDDPDDDEVACSFIVALMQKNMRRQKRDLLTIGYAIYEIPLKAPPGCTHLPGEFFVRNSSRARSHNFINLREVSDRFRLPPGHYVIVPSTYEPHQEADFLLRVYCE 481
BLAST of Calpain vs. Ensembl Sea Lamprey
Match: capn3a (calpain 3a, (p94) [Source:ZFIN;Acc:ZDB-GENE-040912-97]) HSP 1 Score: 298.901 bits (764), Expect = 8.805e-91 Identity = 193/575 (33.57%), Postives = 290/575 (50.43%), Query Frame = 1
Query: 151 LDDSKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK---LCETIICITMLEGSLVNMPDSSFI---YSIFKI-EDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
+D K F + + LS + LF DP F N+ +Y S + +FQW RP E+ P+ I + + +G LGDCW+LAAI ++++ L+ +VI Q F+++Y G F F+ WR G W DVVVDDRLPT N +LV++ S+ +EFWSALLEKA+AK+ GSY+ L GG + +AME TGG+ + F + K P+ ++ +MVKA+ S+M C I+ + + G+ P+ +S ++ P +HIQ+ I E + GL+ HAYS+T + EV + LVR+RNPWG+ VEW G +SD S EWN I E++ L+L DGEFW+SFQDF F+ LEIC ++ D ++ +W G+W + + D F NPQYR + + DP LC I+ + S+ + ++I+++ E+ R L +FF N+S + +++ + R RLPPG YVI+PST E +FLLR+ E E +D
Sbjct: 46 IDTQGKSFEELRTLCLSHDVLFEDPDFPAHNNSIYFSQNAPMQFQWMRPKELCV-PRSIVIEANHTHICEGDLGDCWVLAAIACMTMNEELLARVIPAGQSFEENYAGIFHFQFWRFGEWVDVVVDDRLPTFNNQLVFVQSAERNEFWSALLEKAYAKVHGSYEALKGGNTTEAMEDFTGGVTEYFEM--KNPPKDLYKIMVKATSRRSLMGCSIDDG---MCWRCGVPAPDSIGLVRSNMSPPIL-----------------------------------------RHIQLTPIGIEDAKKRKIAMLTHAESRTPMGLVRNHAYSITGLDEVKHRSKIVQLVRLRNPWGH-VEWIGPWSDASKEWNEITPSEKQRLQLISAEDGEFWISFQDFCRNFTKLEICNLSPDALADDRTNKWTVSVSEGRWVRGCSAGGCRNYPDTFWTNPQYRLQLYDEDDDPDDNELLCSFIVALMQKNRRKERRQGSTMLTIGFAIYEVTEELRGSREHLPKDFFLYNASRARCNSYINLREVTERFRLPPGEYVIVPSTFEAHQEGEFLLRVFAEKHNVSEEMD 572
BLAST of Calpain vs. Ensembl Sea Lamprey
Match: capn9 (calpain 9 [Source:ZFIN;Acc:ZDB-GENE-010724-2]) HSP 1 Score: 171.4 bits (433), Expect = 4.309e-46 Identity = 85/173 (49.13%), Postives = 116/173 (67.05%), Query Frame = 1
Query: 283 QWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLF--RLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
+W EI +NP+FI DG +R D+ QG LGDCWLLAAI SL+L+ L+ +V+ Q F Y G F F + W G W DV +DDRLPT+NG+LV++HS++ EFWSALLEKA++KL GSY+ L GG +AME TGG+ ++ L + A P ++ +M KA S+M C I+
Sbjct: 3 KWGLSREIVQNPEFIVDGATRTDIKQGALGDCWLLAAIASLTLNDQLLAKVVPPGQNFRDGYAGIFHFQQKFWHFGEWMDVFIDDRLPTKNGQLVFVHSADKREFWSALLEKAYSKLNGSYEALKGGSIAEAMEDFTGGVYELLDLKT-APPDLFRIMCKARQKGSMMGCSID 174 HSP 2 Score: 160.229 bits (404), Expect = 3.499e-42 Identity = 100/266 (37.59%), Postives = 141/266 (53.01%), Query Frame = 1
Query: 1075 QREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI--NTGN-KQWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPG-----KLCETIICITMLEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCE-PELSMESVD 1821
+ E + GL++GHAYS+T V EV ++ L+RIRNPWG EVEW GA+SD++PEW +++ ER L+ DGEFWMSF DF+ +F+ +EIC +T D G+ + W G+W + + + F NPQ+R L NE D K C I+ + + Y+I+KI D + EFF N+S + F++ + R RLP G YVI+PST EADFL+R+ E P S ES D
Sbjct: 180 ESEARTSNGLVKGHAYSITGVDEVNYRGQKVKLLRIRNPWG-EVEWNGAWSDHAPEWKALDQGERNRLQQVMAEDGEFWMSFDDFMRHFTKMEICNLTPDRVEREGDPRYWTVTVHDGRWLKGCTAGGCRNFPETFWTNPQFRLK-LHNEDDEADETGSKACSFIVALMQKNMRKQQRNMLTMGYAIYKIPDQ---TGPMPKEFFLYNASKARSPSFINLREVSQRFRLPAGEYVIVPSTFEPHQEADFLVRVFAEKPNRSKESDD 440
BLAST of Calpain vs. Ensembl Sea Lamprey
Match: capn1a (calpain 1, (mu/I) large subunit a [Source:ZFIN;Acc:ZDB-GENE-040426-1263]) HSP 1 Score: 170.244 bits (430), Expect = 5.033e-45 Identity = 93/223 (41.70%), Postives = 133/223 (59.64%), Query Frame = 1
Query: 145 KNLDDSKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCS------SDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPI 792
+N K F KK L + LF DP F S L+ + + +WKRP E+ ++P+FI G +R D+ QG L DCWLL+AI SL+L +L+ +V+ + Q F + Y G FLFR + G W DV VDDRLPT+ G+LV++ S +EFWSALLEKA+AKL G Y+ L G+ +A+ TGG+ + F + + +PR WD + +A +I+CC I
Sbjct: 21 RNFKTKSKTFQEIKKECLQRKILFEDPDFPASLSSLHIRVQPTVITQSNNFIEWKRPWELVRHPEFIVSGATRADINQGTLPDCWLLSAIASLTLYPDLLAKVVPMGQSFHEDYAGIFLFRFCQYGEWVDVHVDDRLPTKKGELVFVQSGTGNEFWSALLEKAYAKLNGCYEALAYGFPVEALCDFTGGVGETFTMNN--APRDYWDGISRAIKKGAIICCGI 241 HSP 2 Score: 114.775 bits (286), Expect = 7.454e-27 Identity = 79/246 (32.11%), Postives = 120/246 (48.78%), Query Frame = 1
Query: 1099 GLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNKQ--WKTYNEFGKWSQNLGSDN---------FHLNPQYRFTILV--NEKDPGKLCETIICITMLEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCE 1794
GL+EGHAYS+T V ++ + L+R+RNP+G +VE+ G +SD S WN + E ++K L + DGEFW+S +DF F +EIC+ S++ +++ W W + L + F LN Q+ T+L N D C ++ +T F+ I A +L + + ++ EF++ + R LPPGNY+IIP T G EA FLLRI E
Sbjct: 257 GLVEGHAYSITGVIKLKFPGKTVQLIRVRNPYG-KVEYTGPWSDGSTTWNEVPEEDQKRLHVR-AEDGEFWISDKDFRKNFHLVEICHQGSEVEIEHRKNLWICELHNSMWLKGLNAGGSRRHRVFKEFPLNYQFFLTLLEEDNVMDVCHGCHFLLSLTQKHRRKFRKIGIDFVPIGIYIYKAVVPGKKLTPQQLRKEKPLVESPEFIARREVTMRMTLPPGNYIIIPCTESAGQEAQFLLRIFME 500
BLAST of Calpain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007888.1 (pep scaffold:Pmarinus_7.0:GL477427:174304:193458:1 gene:ENSPMAG00000007039.1 transcript:ENSPMAT00000007888.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 138.658 bits (348), Expect = 7.182e-35 Identity = 88/251 (35.06%), Postives = 134/251 (53.39%), Query Frame = 1
Query: 1102 LIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK--QWKTYNEFGKWSQNL---GSDNF--HLNPQYRFTILVNEKDPGKL-----CETIICITMLEGSLVNMPDSSFI---YSIFKIEDARYLNAQ--LDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPE 1800
L++GHAYS+T EV + LVRIRNPWG +VEW GA+SDN+P+W +++ +R L+++ DGEFWMSF+DF+ FS LEIC +T D + W N G W + G NF HL +++F + E+D C I+ + E + Y+I+++ + Q L ++F+ +SS + E F++ + R LP G Y+++PST EADF+LRI E +
Sbjct: 72 LVKGHAYSLTGTTEVDYQGQMVRLVRIRNPWG-QVEWTGAWSDNAPQWGYVSDEDRGRLQVS-QEDGEFWMSFEDFLRQFSRLEICNLTPDALDDDSLGHWSANNFEGSWVRGCSAGGCRNFIVHLWEKWKFHLKHTEEDDDPHDNEAGCTFIVALMQKERRKKRRQGGDVLTVGYAIYEVPEQFVGQPQVKLKKDYFQYHSSKARSEVFINLREVSQRFTLPAGEYIVVPSTFEPNQEADFILRIFSEKQ 320
BLAST of Calpain vs. Ensembl Nematostella
Match: EDO40193 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7W7]) HSP 1 Score: 181.8 bits (460), Expect = 6.520e-49 Identity = 92/217 (42.40%), Postives = 133/217 (61.29%), Query Frame = 1
Query: 184 KKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYF---DKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKL 825
++ + S + F D F + ++ S F+WKRP +I ++P G SR D+ QG+LGDCWLLAAI +L+ S LMN+V+ D D++Y GA F +W+ G W DV++DDRLPT NGKLV++HSS +EFWSALLEKA+AKL GSY++L GG + +A+E TGG+ + F L ++ +M A ++M C I ++ KL
Sbjct: 10 RRQVRSTGKPFEDDEFLAIDSSIFFSKRPPRPFEWKRPSDICEDPHMFVGGASRLDIQQGMLGDCWLLAAIAALTQHSALMNKVVPQDYVANPNDRNYTGALKFCIWQYGEWKDVIIDDRLPTYNGKLVFVHSSERNEFWSALLEKAYAKLNGSYESLKGGQTGEALEDFTGGLAESFDLNKNVPKDLFCIMQTAEKRHALMGCSIAAKANEIEAKL 226 HSP 2 Score: 169.859 bits (429), Expect = 9.880e-45 Identity = 94/264 (35.61%), Postives = 150/264 (56.82%), Query Frame = 1
Query: 1081 EIKVDTGLIEGHAYSVTNVKEVCSLEGQ----INLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSD--INTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLV---NMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
E K++ GL++GHAY+VT ++V + + + LVR+RNPWGNE EWKGA+ D S EW +++ E+K + L F +DGEFWMSF DF +F++LEIC + +D + + +++ +E G W + L + FH NPQ+R T+ ++ D LC II + + + + +SI+K+E+ + LD EFF+ ++S + F++ + R LPPG YV+IP+T E +F+LR+ E VD
Sbjct: 223 EAKLNNGLVKGHAYTVTGCRQVKAKTRRGDLDVELVRVRNPWGNEREWKGAWGDGSSEWQLLSDYEKKSIGLTFEDDGEFWMSFDDFKKHFTTLEICMLGADSAMEGSSVGFESRSEHGMWRRGLTAGGCRNFIKTFHKNPQFRITLQDSDDDADDLCTVIIALMQKDRRKEKKHGINPLTIGFSIYKLEEEKDKQGNLDMEFFQYHASTARSPVFINSREVVGRYALPPGEYVVIPTTFNPNEEGNFVLRMYSEKAHKTCEVD 486
BLAST of Calpain vs. Ensembl Nematostella
Match: EDO48633 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ83]) HSP 1 Score: 176.407 bits (446), Expect = 3.736e-47 Identity = 102/263 (38.78%), Postives = 153/263 (58.17%), Query Frame = 1
Query: 1075 QREIKVDTGLIEGHAYSVTNVKE--VCSLEG--QINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGN--KQWKTYNEFGKWSQNLGSD-------NFHLNPQYRFTILVNEKDPGKLCETIICITMLEGS-LVNMPDSSFI-YSIFKIEDARYLNAQLDSEFFKTNSSY-LKYEFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCE-PELSME 1812
Q E ++ GLI HAYS+T +++ + SL G +++L+R+RNP N EW+GA+SD SPEWN ++ E++ L L F++DGEFWMSF DF+ F+ LEIC +T D N ++W NE G+W + + + +F+ NPQ+R + + D +LC I+ + ++ S V +PD + I ++I+K +LD FF+ N + + F I SR RL G YV+IPST G E DFLLRI E P+ + E
Sbjct: 226 QYEGTLENGLITRHAYSITGLRKATLSSLRGRPEVDLIRLRNPHANSSEWRGAWSDRSPEWNMLSSQEKRELNLTFDDDGEFWMSFDDFLNNFTRLEICMLTPDSMEQNDRRKWDVQNEKGRWQKGVTAGGCRNFIASFYTNPQFRVCLKNPDDDDDELCTVIVSLMRVDHSHRVKIPDRAPIGFAIYKAPSDMRKGDRLDRAFFEYNKAVAMTTSFSKLREISSRYRLHAGEYVVIPSTFNPGQEGDFLLRIFSERPQYASE 488 HSP 2 Score: 161.77 bits (408), Expect = 4.228e-42 Identity = 89/219 (40.64%), Postives = 135/219 (61.64%), Query Frame = 1
Query: 139 VSKNLDDSKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDK-SYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPI 792
+S+ ++ F S ++ LL+ +LF D F SN +L+ + + WKRP EI K+PQFI G S DV QG + +CW +AA+ L+ + +L++ V DQ F+ SY G F F W+ G W +++VDD LPT NG+L + S + DEFWSALLEKA+AKL GSY+ + G +A++ +TGG+I+ L + +++VM+KA +S+M C I
Sbjct: 1 MSRRALHKRERFASLQRQLLNSKRLFEDRDFPASNRVLFTRRRSV-QLIWKRPHEISKSPQFIARGFSSSDVAQGRIRNCWFVAALADLTTNIDLLHHVCPPDQSFEPGSYCGMFRFNFWQFGEWVEIIVDDYLPTYNGRLYFTKSDDPDEFWSALLEKAYAKLCGSYEAMSLGRISEALQDMTGGMIENVDLQNPPHG-LFNVMMKAYERQSLMGCSI 217
BLAST of Calpain vs. Ensembl Nematostella
Match: EDO48536 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ84]) HSP 1 Score: 151.369 bits (381), Expect = 1.304e-38 Identity = 74/169 (43.79%), Postives = 108/169 (63.91%), Query Frame = 1
Query: 301 EIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQY----FDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
EI P+F DG SR D+ QG+LG+CWLLAA+ L+ L+++++ Y F Y G +F+LW+ G W V++DDRLPT GKLV++HS +EFW ALLEKA+AK+ GSY+ L GG + +A+E TGG+ ++F L A ++ +M +A ++M C IN
Sbjct: 12 EICSEPRFYVDGASRLDIKQGVLGNCWLLAAMSVLAQHKPLVDRIMPEGVYEADRFSPRYTGKVVFKLWQYGEWVTVIIDDRLPTYKGKLVFVHSPEKNEFWCALLEKAYAKIHGSYEALKGGLTGEALEDFTGGLSEVFVL-EDAPANLFQIMQRAEKRSALMGCSIN 179 HSP 2 Score: 108.612 bits (270), Expect = 1.152e-24 Identity = 54/133 (40.60%), Postives = 82/133 (61.65%), Query Frame = 1
Query: 1150 SLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNKQWKTYN---EFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCE 1518
S G + L+RIRNPWG+E EW GA+ D SPEW +++ E++ L+L F+ DGEFWMSF+DF ++F LE+C + + N +K ++N E G W +++ + + FH NPQ+R LV+ +CE
Sbjct: 183 STRGGVELIRIRNPWGDEKEWTGAWGDRSPEWQELSQDEKRSLDLEFDVDGEFWMSFEDFKSHFDKLEVCMLDVE-NAIDKTQISFNSRLENGFWRKHVSAGGCRNFLETFHTNPQFR---LVSRVMSRMICE 311 HSP 3 Score: 62.3882 bits (150), Expect = 3.438e-10 Identity = 32/88 (36.36%), Postives = 53/88 (60.23%), Query Frame = 1
Query: 1582 YSIFKIEDA---RYLNAQLDSEFFKTNSSYLKYEFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIF 1836
++++KI+D +Y ++LD E+F+ N S + F++ + R L PG YVI+PST ++ EADFLLRI E +S ++ + F
Sbjct: 395 FALYKIDDITKRKYGVSRLDKEYFQFNKSTERSTFINSREVVGRFELAPGEYVIVPSTFIQNAEADFLLRIFSEKAVSSSALTESTFF 482
BLAST of Calpain vs. Ensembl Nematostella
Match: EDO34860 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SN59]) HSP 1 Score: 140.198 bits (352), Expect = 6.357e-35 Identity = 84/189 (44.44%), Postives = 109/189 (57.67%), Query Frame = 1
Query: 178 SQKKVLLSKN-QLFHDPFFCP-SNDLLYCSSDHLDKFQWKRPLEI--RKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILV------DQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRL 714
S+ K KN QLF D F P ++ L Y S + +WKRP EI P DG DV QG LG+CW +AA SL+L L+ +VI D ++Y G F FR +R+G W +VVVDD LPT NGKLVY+HS +EFWSAL+EKA+AKL G+Y+ L G + A+ TGG+ + L
Sbjct: 13 SKLKAECEKNEQLFVDSVFKPETSSLYYSRSCPPEPVEWKRPSEICHPAVPHLFVDGADSHDVTQGKLGNCWFVAACSSLALRPFLLYKVIPDKDKQEWDPKKPENYQGIFRFRFYRQGEWTEVVVDDLLPTVNGKLVYIHSKEKNEFWSALIEKAYAKLAGTYEALAAGSTADALVDFTGGVAEAISL 201 HSP 2 Score: 131.724 bits (330), Expect = 3.921e-32 Identity = 87/255 (34.12%), Postives = 131/255 (51.37%), Query Frame = 1
Query: 1075 QREIKVDTGLIEGHAYSVTNVKEVCSLEGQINL--------VRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSD--INTGNKQWKTYNEFGKWS--QNLGS-----DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKYEFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRIL 1788
+ E ++DTGLI+GHAY VT VK+V EG ++L +R+RNPWG E EW G +SD S EW + +RK + + F NDGEFWM F DF F+ +C+V + I+ KQW Y W+ QN G D F NPQY F+I NE + + +++ + + + + + I K+E+ R ++ S F K S F++ + +R L G Y+++PST F++RI
Sbjct: 241 EMEQRLDTGLIKGHAYGVTMVKKVTVGEGLLSLFNKKHFYMIRLRNPWG-EKEWNGRWSDESEEWKKLKPGDRKKMGIVFENDGEFWMEFDDFCREFTDCTLCHVINKAMIHKVFKQWHIYKHNYNWTQGQNAGGCVQNQDTFLKNPQYAFSITDNEGEDAMV--SLMQEDRRKDKSKGVGNLTIGFYIMKVEENR--KYRVHSMFPKAGDSI----FINAREVMNRFFLAAGRYLVVPSTYEPHTPGHFMMRIF 486
BLAST of Calpain vs. Ensembl Nematostella
Match: EDO37991 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SE78]) HSP 1 Score: 88.1965 bits (217), Expect = 3.280e-18 Identity = 59/171 (34.50%), Postives = 90/171 (52.63%), Query Frame = 1
Query: 214 FHDPFFCPSNDLLYCS---SDHLDKFQWKRPLEI------RKN-PQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPT-ENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGG 693
F D F P LY + S+H W RP EI R N P + D++QG+LGDCW L+++ L+ L+ ++++ + S GA+ RL ++G W +++DD LP + G+ VY + + + W L+EKA AKL GSY+ L G S + + TLTG
Sbjct: 44 FVDDSFPPVLKSLYINPRHSEHPRVADWLRPNEIDNFSTCRTNIPWAVFRNPRPSDIIQGVLGDCWFLSSLAVLAERPELLEKILITREV---SPQGAYQVRLCKDGKWTVILIDDLLPCNQRGQPVYSWA-HRRQLWVPLIEKAMAKLHGSYEALTAGRSIEGLATLTGA 210 HSP 2 Score: 68.1662 bits (165), Expect = 6.763e-12 Identity = 37/90 (41.11%), Postives = 54/90 (60.00%), Query Frame = 1
Query: 1096 TGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYV 1365
GL HAYSV +V++V I L+R+RNPWG + W G +SD S W E+ R L ++ +G FWMS +DF+ YF S+++C +
Sbjct: 268 VGLQTHHAYSVLDVRDVHG----IRLLRLRNPWG-QYSWTGDWSDASSLWTP--EL-RHELMVHGAQEGVFWMSLEDFMKYFDSVDVCKI 349
BLAST of Calpain vs. Ensembl Medaka
Match: capn3b (calpain-3 [Source:NCBI gene;Acc:101169329]) HSP 1 Score: 309.686 bits (792), Expect = 2.470e-94 Identity = 200/552 (36.23%), Postives = 281/552 (50.91%), Query Frame = 1
Query: 166 KWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNKQ--WKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK---LCETIICITMLEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLR 1782
K F + + K LF DP F ++ L+ S K +WKRP EI +NPQFI DG +R D+ QG LGDCWLLAAI L+L L+ +VI +Q F ++Y G F F+ WR G W D+VVDDR+PT N LV+ S +EFWSALLEKA+AKL GSY+ L GG + AME TGG+ ++F L S+A ++ +M KA S+M C I+ S + P N+ I+ + +S LC H N I E + + GL+ GHAYS+ ++EV S +G+I L+R+RNPWG V WKG +S NS EW+ ++ +R L+ EFWMSF DF F+ LE+C +T D TG+++ W G+W + + D F NPQYR L E DP + LC ++ + + + F+ F I + + N L +FF N+S K + +++ + R RLPPG YVI+P+T E +FLLR
Sbjct: 61 KTFLELRDKYVKKKVLFEDPLFPANDSSLFYSHKPSMKIEWKRPTEICENPQFIIDGANRTDICQGELGDCWLLAAIACLTLSEKLLYRVIPPEQSFTENYAGIFHFQFWRYGEWIDIVVDDRIPTCNNLLVFTKSFRENEFWSALLEKAYAKLHGSYEALKGGNTLDAMEDFTGGVTELFEL-SEAPKGLFIIMKKALERGSLMGCSIDVSGQEKC-------PGKSNNMHLYIHSQIENKMLSCLCIV--------------------------------HQVTTANDI--------------ESRTNQGLVRGHAYSIIALEEV-SEDGKIRLIRLRNPWG-WVLWKGPWSVNSKEWSTVSTADRDNLKKQTLEMSEFWMSFDDFKKNFTKLEMCNLTPDALTGDERQSWTVSINEGRWVRGSSAGGCRNFPDTFWTNPQYRLR-LYEEDDPEEEQVLCTVVVALMQKGRRMQRHAGAKFLTIGFSIYELQGQNQHLQKDFFLYNASKAKCKAYINLREVTERFRLPPGEYVILPTTFKAHEEGEFLLR 555
BLAST of Calpain vs. Ensembl Medaka
Match: zgc:55262 (calpain-1 catalytic subunit [Source:NCBI gene;Acc:101155642]) HSP 1 Score: 241.121 bits (614), Expect = 1.469e-69 Identity = 169/572 (29.55%), Postives = 266/572 (46.50%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFF-CPSNDL----LYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNN--KIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI--NTGNKQWKTYNEFGKWSQNLGSD-------NFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPD---SSFIYSIFKIED--ARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
+ S K L +LF D F C ++ L L S +W RP E+ K P+FI DG +R D+ QG LGDCWLLAAI SL+L+ NL+++V+ Q F + Y G F F+ W+ G W DVV+DDRLP ++GKL+++HS EFWSALLEKA+AKL G Y+ L GG + + E TGG+ +++ L K P ++ ++ +A S++ C I+ I + A++F ++ G+ YS T + N ++V+ N PWG + PW ++ S EW+ ++ R L+ N + DGEFWM+F DF+ FS +EIC +T+D NT K+W + G+W + + F LNPQ++ ++ + C ++ + + + ++++++ D N L +FF T++S + E F++ + SR RLP G Y+++PST EADF+LR+ E E +D
Sbjct: 38 YESLKAQCLRSGKLFEDNLFPCAASSLGFNELGPRSSKTYGVRWMRPTELCKRPEFIVDGATRTDICQGALGDCWLLAAIASLTLNDNLLHRVVPHGQCFKQGYAGIFHFQFWQFGEWVDVVIDDRLPAKDGKLLFVHSVEGTEFWSALLEKAYAKLNGCYEALSGGSTSEGFEDFTGGVTEMYDL-IKPPPDLFGIISRAVERGSLLGCSID------------------------ITSSTDREAVTF---------------------KKLVKGHAYSLTGVDEVMYRGNLTRLVRMRN-PWGE-----------------------------------MEWTGPWSDD----------SKEWSYVDSSVRDRLQ-NRSEDGEFWMAFSDFLREFSRVEICNLTADALDNTQMKKWSSSLFQGEWRRGSTAGGCRNYPATFWLNPQFKISLQHPDVSGKPDCSFLVGLMQKDRRKKRQEGKDMETIGFALYEVPDEFKGSSNVHLKRDFFLTHASSARSELFINLREVSSRLRLPAGEYIVVPSTFEPNKEADFVLRVFSEKPADSEELD 516
BLAST of Calpain vs. Ensembl Medaka
Match: zgc:55262 (calpain-1 catalytic subunit [Source:NCBI gene;Acc:101155642]) HSP 1 Score: 239.965 bits (611), Expect = 2.219e-69 Identity = 173/592 (29.22%), Postives = 274/592 (46.28%), Query Frame = 1
Query: 172 FNSQKKVLLSKNQLFHDPFF-CPSNDL----LYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNN--KIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI--NTGNKQWKTYNEFGKW---SQNLGSDN----FHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPD---SSFIYSIFKIED--ARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPINFCQQ*NDVQL 1881
+ S K L +LF D F C ++ L L S +W RP E+ K P+FI DG +R D+ QG LGDCWLLAAI SL+L+ NL+++V+ Q F + Y G F F+ W+ G W DVV+DDRLP ++GKL+++HS EFWSALLEKA+AKL G Y+ L GG + + E TGG+ +++ L K P ++ ++ +A S++ C I+ I + A++F ++ G+ YS T + N ++V+ N PWG + PW ++ S EW+ ++ R L+ N + DGEFWM+F DF+ FS +EIC +T+D NT K+W + G+W S G N F LNPQ++ ++ + C ++ + + + ++++++ D N L +FF T++S + E F++ + SR RLP G Y+++PST EADF+LR+ E + + L + + C D+++
Sbjct: 38 YESLKAQCLRSGKLFEDNLFPCAASSLGFNELGPRSSKTYGVRWMRPTELCKRPEFIVDGATRTDICQGALGDCWLLAAIASLTLNDNLLHRVVPHGQCFKQGYAGIFHFQFWQFGEWVDVVIDDRLPAKDGKLLFVHSVEGTEFWSALLEKAYAKLNGCYEALSGGSTSEGFEDFTGGVTEMYDL-IKPPPDLFGIISRAVERGSLLGCSID------------------------ITSSTDREAVTF---------------------KKLVKGHAYSLTGVDEVMYRGNLTRLVRMRN-PWGE-----------------------------------MEWTGPWSDD----------SKEWSYVDSSVRDRLQ-NRSEDGEFWMAFSDFLREFSRVEICNLTADALDNTQMKKWSSSLFQGEWRRGSTAGGCRNYPATFWLNPQFKISLQHPDVSGKPDCSFLVGLMQKDRRKKRQEGKDMETIGFALYEVPDEFKGSSNVHLKRDFFLTHASSARSELFINLREVSSRLRLPAGEYIVVPSTFEPNKEADFVLRVFSEKPADSDCLCLLLDIVRVTDLCLCQQDMEI 536
BLAST of Calpain vs. Ensembl Medaka
Match: capn9 (calpain 9 [Source:NCBI gene;Acc:101158662]) HSP 1 Score: 194.126 bits (492), Expect = 7.329e-53 Identity = 100/211 (47.39%), Postives = 137/211 (64.93%), Query Frame = 1
Query: 166 KWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPR-IWDVMVKASHMESIMCCPIN 795
K F ++ L K LF DP F + L+ S + QWKRP EI +NP+FI G R D+ QG LGDCWLLAAI SL+L + M +V+ DQ FD +Y G F F+ W+ W DVVVDDRLPT +L+ +HS++ DEFWSALLEKA+AKL GSY++L GG + +AME TGG+ +++ +K++P ++ +M KA S+M C I+
Sbjct: 25 KTFEQLRQECLQKGVLFEDPDFPAVDSSLFYSQTVPVQIQWKRPKEICENPKFIVGGADRTDICQGQLGDCWLLAAIASLTLKKDAMARVVPPDQDFDHNYAGIFHFQFWQHNKWLDVVVDDRLPTVRNRLIMLHSASNDEFWSALLEKAYAKLNGSYESLKGGSTMEAMEDFTGGVGEMYE--TKSAPENLFSIMKKALDRGSMMGCSID 233 HSP 2 Score: 142.895 bits (359), Expect = 2.860e-35 Identity = 81/253 (32.02%), Postives = 132/253 (52.17%), Query Frame = 1
Query: 1066 TPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDI--NTGNKQWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCE 1794
+ + E K TGL++GHAYS+T ++E+ + L+R+RNPWG +VEW GA+SDNS EWN I++ E+ + N DGEFWM F+DF + +EIC +T D + + W G W + + D F NPQ+R + + D +C +I + + F + +A +Q++ +FF+ + S + +++ + R LPPG Y+++P+T EADF++RI E
Sbjct: 236 SSAESEAKTSTGLVKGHAYSITGMEELQFRGQTVKLIRVRNPWG-QVEWNGAWSDNSREWNYIDKKEKDRILQNSLEDGEFWMEFEDFKKNYDKVEICNMTPDALDDDTKRHWAVNVFEGNWIRGSTAGGCRNYIDTFWTNPQFRLQLEDADDDDDDVCSVVIALMQKNRRKLRKEGLDLETIGFAVYEAPDDESQVEKDFFRYHPSKARSRTYINMREVSERFTLPPGKYLLVPTTYQPHQEADFIIRIFSE 487
BLAST of Calpain vs. Ensembl Medaka
Match: capn3b (calpain-3 [Source:NCBI gene;Acc:101169329]) HSP 1 Score: 192.586 bits (488), Expect = 7.564e-52 Identity = 99/200 (49.50%), Postives = 130/200 (65.00%), Query Frame = 1
Query: 196 LSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPIN 795
+ K LF DP F ++ L+ S K +WKRP EI +NPQFI DG +R D+ QG LGDCWLLAAI L+L L+ +VI +Q F ++Y G F F+ WR G W D+VVDDR+PT N LV+ S +EFWSALLEKA+AKL GSY+ L GG + AME TGG+ ++F L S+A ++ +M KA S+M C I+
Sbjct: 72 VKKKVLFEDPLFPANDSSLFYSHKPSMKIEWKRPTEICENPQFIIDGANRTDICQGELGDCWLLAAIACLTLSEKLLYRVIPPEQSFTENYAGIFHFQFWRYGEWIDIVVDDRIPTCNNLLVFTKSFRENEFWSALLEKAYAKLHGSYEALKGGNTLDAMEDFTGGVTELFEL-SEAPKGLFIIMKKALERGSLMGCSID 270 HSP 2 Score: 147.902 bits (372), Expect = 9.648e-37 Identity = 94/277 (33.94%), Postives = 149/277 (53.79%), Query Frame = 1
Query: 1081 EIKVDTGLIEGHAYSVTNVKEV--CSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNKQ--WKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGK---LCETIICITMLEGSLVNMPDSSFI---YSIFKI-EDARYLNAQLDSEFFKTNSSYLKYE-FLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINPINF 1854
E + + GL+ GHAYS+ ++E S +G+I L+R+RNPWG V WKG +S NS EW+ ++ +R L+ EFWMSF DF F+ LE+C +T D TG+++ W G+W + + D F NPQYR L E DP + LC ++ + + + F+ +SI+++ ++ + N L +FF N+S K + +++ + R RLPPG YVI+P+T E +FLLR+ E + + E + ++ I PI F
Sbjct: 278 ESRTNQGLVRGHAYSIIALEECDQVSEDGKIRLIRLRNPWG-WVLWKGPWSVNSKEWSTVSTADRDNLKKQTLEMSEFWMSFDDFKKNFTKLEMCNLTPDALTGDERQSWTVSINEGRWVRGSSAGGCRNFPDTFWTNPQYRLR-LYEEDDPEEEQVLCTVVVALMQKGRRMQRHAGAKFLTIGFSIYEVPKELQGQNQHLQKDFFLYNASKAKCKAYINLREVTERFRLPPGEYVILPTTFKAHEEGEFLLRVFSENKSTSEEAETSIDKIEPIVF 552
BLAST of Calpain vs. Planmine SMEST
Match: SMESG000067771.1 (SMESG000067771.1) HSP 1 Score: 1191.41 bits (3081), Expect = 0.000e+0 Identity = 587/588 (99.83%), Postives = 587/588 (99.83%), Query Frame = 1
Query: 58 MAKNEQSRTVSMESNSETVTFDNTEFRVSKNLDDSKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSXXXXXXXXXXTQGKPIKTEISDGYLYSKTNDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNKQWKTYNEFGKWSQNLGSDNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKYEFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 1821
MAKNEQSRTVSMESNSETVTFDNTEFRVSKNLDDSKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSKKRKSIKNKKTQGKPIKTEISDGYLYSK NDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNKQWKTYNEFGKWSQNLGSDNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKYEFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD
Sbjct: 1 MAKNEQSRTVSMESNSETVTFDNTEFRVSKNLDDSKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQKLGLFIPNPKNSDKSVINEPKKKSAISFLCFSKKRKSIKNKKTQGKPIKTEISDGYLYSKINDKHIQINNNKIVKEPNEPWGTPVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNKQWKTYNEFGKWSQNLGSDNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKYEFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD 588
BLAST of Calpain vs. Planmine SMEST
Match: SMESG000067761.1 (SMESG000067761.1) HSP 1 Score: 276.559 bits (706), Expect = 1.571e-82 Identity = 135/243 (55.56%), Postives = 173/243 (71.19%), Query Frame = 1
Query: 94 ESNSETVTFDNTEFRVSKNLDDSKKWFNSQKKVLLSKNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDKSYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPINHSQVQLAQK 822
+++SE++ + F VSK+L +SK WF +QK++L +KN LF DPFF +N LY K +WKRP E+++N FI DG SRFDVVQG LGDCWLLAA+ SLS+ LM QVI DQ FD+ YVGAF+FRLW+ G WYDVVVDDRLPT G+LVY+HS +L+EFWSALLEKA+AK+ GSY+ L GG S +AME TGGI ++F LGS A P +WD+M+K S+M C I ++ K
Sbjct: 32 QTDSESIY--GSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMYQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAK 272 HSP 2 Score: 214.157 bits (544), Expect = 8.891e-60 Identity = 111/277 (40.07%), Postives = 167/277 (60.29%), Query Frame = 1
Query: 1069 PVQREIKVDTGLIEGHAYSVTNVKEVCSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICYVTSDINTGNK------QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDSSFI----YSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCEPELSMESVD*TLIFINP 1845
P + E K + GLI+GHAYS+T+VK V S G++NLVR+RNPWGN EW GAYSD SPEWN I++VERK L L F +DGEFWM+FQDF+ YF+ LEIC+++ + NK +W+ E G+W +N+ + + FHLNPQ++ ++ +++ + T+I M + + + Y+I++++D Y + LD +F+ +S+ K F++ +C R +LP G YVI+PST +G EA FLLRI E + +D + P
Sbjct: 266 PTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAP 542
BLAST of Calpain vs. Planmine SMEST
Match: SMESG000039299.1 (SMESG000039299.1) HSP 1 Score: 213.772 bits (543), Expect = 3.295e-59 Identity = 109/213 (51.17%), Postives = 140/213 (65.73%), Query Frame = 1
Query: 202 KNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDK-SYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPI----NHSQVQLAQKL 825
+ +L+ DP F N+ LY S K WKRP EI +NP+FI +G SRFDV QG LGDCWLLAA+ LSL S L NQV+ DQ F K SY G F FR WR G W +V+VDDRLPT N KLVYMHS++ +EFWSALLEKA+AK+ GSY++L GG + +A+E TGG+ + F + + ++D+M KA S+M C I ++ QL L
Sbjct: 109 RGKLYEDPDFPAVNESLYFSQRPPRKIDWKRPHEICQNPEFISEGASRFDVRQGELGDCWLLAAVACLSLHSELFNQVVPSDQNFSKPSYCGMFRFRFWRFGAWKEVLVDDRLPTYNNKLVYMHSTDSNEFWSALLEKAYAKISGSYESLKGGSTSEALEDFTGGVTEFFDMKKEVPKNLFDIMFKAFSRCSLMGCSIEADPRKTEAQLPNGL 321 HSP 2 Score: 174.096 bits (440), Expect = 1.699e-45 Identity = 105/265 (39.62%), Postives = 151/265 (56.98%), Query Frame = 1
Query: 1069 PVQREIKVDTGLIEGHAYSVTNVKEV----CSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICY-----VTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDS----SFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCE 1794
P + E ++ GLI GHAYS+T+VK V G+I L+RIRNPWGNE EWKG++SD SPEWN I++ +R + L F++DGEFWMS+QDFI F +EIC+ + +D G K +W+ E G+W + L + D F NPQ+R ++ ++D + TII M + + + Y+I++++D LD +FK N S K F++ +C R +LPPG YVI+PST EA FL+RI E
Sbjct: 310 PRKTEAQLPNGLIMGHAYSITDVKMVNINIPGKSGKIQLIRIRNPWGNEAEWKGSWSDKSPEWNYISQADRNTMGLTFDDDGEFWMSYQDFIKNFEKMEICHLGPQSLEADQLGGKKKSRWEMCIENGEWKKRLNAGGCRNFLDTFWTNPQFRVKVVDPDEDDDENLGTIIIGLMQKDMRKRRKEGVEFYTIGYAIYQLKDEN--QGPLDINYFKYNGSVAKAPSFINLREVCGRHKLPPGTYVIVPSTFEPNQEASFLMRIFSE 572
BLAST of Calpain vs. Planmine SMEST
Match: SMESG000039299.1 (SMESG000039299.1) HSP 1 Score: 213.386 bits (542), Expect = 3.920e-59 Identity = 106/198 (53.54%), Postives = 135/198 (68.18%), Query Frame = 1
Query: 202 KNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDK-SYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPI 792
+ +L+ DP F N+ LY S K WKRP EI +NP+FI +G SRFDV QG LGDCWLLAA+ LSL S L NQV+ DQ F K SY G F FR WR G W +V+VDDRLPT N KLVYMHS++ +EFWSALLEKA+AK+ GSY++L GG + +A+E TGG+ + F + + ++D+M KA S+M C I
Sbjct: 109 RGKLYEDPDFPAVNESLYFSQRPPRKIDWKRPHEICQNPEFISEGASRFDVRQGELGDCWLLAAVACLSLHSELFNQVVPSDQNFSKPSYCGMFRFRFWRFGAWKEVLVDDRLPTYNNKLVYMHSTDSNEFWSALLEKAYAKISGSYESLKGGSTSEALEDFTGGVTEFFDMKKEVPKNLFDIMFKAFSRCSLMGCSI 306 HSP 2 Score: 174.096 bits (440), Expect = 1.762e-45 Identity = 105/265 (39.62%), Postives = 151/265 (56.98%), Query Frame = 1
Query: 1069 PVQREIKVDTGLIEGHAYSVTNVKEV----CSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICY-----VTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDS----SFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCE 1794
P + E ++ GLI GHAYS+T+VK V G+I L+RIRNPWGNE EWKG++SD SPEWN I++ +R + L F++DGEFWMS+QDFI F +EIC+ + +D G K +W+ E G+W + L + D F NPQ+R ++ ++D + TII M + + + Y+I++++D LD +FK N S K F++ +C R +LPPG YVI+PST EA FL+RI E
Sbjct: 310 PRKTEAQLPNGLIMGHAYSITDVKMVNINIPGKSGKIQLIRIRNPWGNEAEWKGSWSDKSPEWNYISQADRNTMGLTFDDDGEFWMSYQDFIKNFEKMEICHLGPQSLEADQLGGKKKSRWEMCIENGEWKKRLNAGGCRNFLDTFWTNPQFRVKVVDPDEDDDENLGTIIIGLMQKDMRKRRKEGVEFYTIGYAIYQLKDEN--QGPLDINYFKYNGSVAKAPSFINLREVCGRHKLPPGTYVIVPSTFEPNQEASFLMRIFSE 572
BLAST of Calpain vs. Planmine SMEST
Match: SMESG000039299.1 (SMESG000039299.1) HSP 1 Score: 213.001 bits (541), Expect = 4.209e-59 Identity = 106/198 (53.54%), Postives = 135/198 (68.18%), Query Frame = 1
Query: 202 KNQLFHDPFFCPSNDLLYCSSDHLDKFQWKRPLEIRKNPQFIKDGRSRFDVVQGLLGDCWLLAAIGSLSLDSNLMNQVILVDQYFDK-SYVGAFLFRLWREGVWYDVVVDDRLPTENGKLVYMHSSNLDEFWSALLEKAFAKLKGSYKNLDGGYSWKAMETLTGGIIKIFRLGSKASPRIWDVMVKASHMESIMCCPI 792
+ +L+ DP F N+ LY S K WKRP EI +NP+FI +G SRFDV QG LGDCWLLAA+ LSL S L NQV+ DQ F K SY G F FR WR G W +V+VDDRLPT N KLVYMHS++ +EFWSALLEKA+AK+ GSY++L GG + +A+E TGG+ + F + + ++D+M KA S+M C I
Sbjct: 109 RGKLYEDPDFPAVNESLYFSQRPPRKIDWKRPHEICQNPEFISEGASRFDVRQGELGDCWLLAAVACLSLHSELFNQVVPSDQNFSKPSYCGMFRFRFWRFGAWKEVLVDDRLPTYNNKLVYMHSTDSNEFWSALLEKAYAKISGSYESLKGGSTSEALEDFTGGVTEFFDMKKEVPKNLFDIMFKAFSRCSLMGCSI 306 HSP 2 Score: 174.096 bits (440), Expect = 1.686e-45 Identity = 105/265 (39.62%), Postives = 151/265 (56.98%), Query Frame = 1
Query: 1069 PVQREIKVDTGLIEGHAYSVTNVKEV----CSLEGQINLVRIRNPWGNEVEWKGAYSDNSPEWNNINEVERKGLELNFNNDGEFWMSFQDFITYFSSLEICY-----VTSDINTGNK--QWKTYNEFGKWSQNLGS-------DNFHLNPQYRFTILVNEKDPGKLCETIICITMLEGSLVNMPDS----SFIYSIFKIEDARYLNAQLDSEFFKTNSSYLKY-EFLSEGMICSRCRLPPGNYVIIPSTAVKGIEADFLLRILCE 1794
P + E ++ GLI GHAYS+T+VK V G+I L+RIRNPWGNE EWKG++SD SPEWN I++ +R + L F++DGEFWMS+QDFI F +EIC+ + +D G K +W+ E G+W + L + D F NPQ+R ++ ++D + TII M + + + Y+I++++D LD +FK N S K F++ +C R +LPPG YVI+PST EA FL+RI E
Sbjct: 310 PRKTEAQLPNGLIMGHAYSITDVKMVNINIPGKSGKIQLIRIRNPWGNEAEWKGSWSDKSPEWNYISQADRNTMGLTFDDDGEFWMSYQDFIKNFEKMEICHLGPQSLEADQLGGKKKSRWEMCIENGEWKKRLNAGGCRNFLDTFWTNPQFRVKVVDPDEDDDENLGTIIIGLMQKDMRKRRKEGVEFYTIGYAIYQLKDEN--QGPLDINYFKYNGSVAKAPSFINLREVCGRHKLPPGTYVIVPSTFEPNQEASFLMRIFSE 572 The following BLAST results are available for this feature:
BLAST of Calpain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Calpain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Calpain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Calpain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Calpain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Calpain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Calpain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Calpain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30034337 ID=SMED30034337|Name=Calpain|organism=Schmidtea mediterranea sexual|type=transcript|length=2041bpback to top protein sequence of SMED30034337-orf-1 >SMED30034337-orf-1 ID=SMED30034337-orf-1|Name=SMED30034337-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=589bp MAKNEQSRTVSMESNSETVTFDNTEFRVSKNLDDSKKWFNSQKKVLLSKNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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