SMED30034335

Overview
NameSMED30034335
Smed IDSMED30034335
Length (bp)3316
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30034335 (SMED30034335) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30034335 (SMED30034335) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30034335 (SMED30034335) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30034335 (SMED30034335) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30034335

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30034335SMESG000009102.1 SmedASXL_015630SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30034335SMESG000009102.1 SmedASXL_015630SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
protonephridiaSMED30034335SMESG000009102.1 dd_Smed_v4_8341_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30034335SMESG000009102.1 dd_Smed_v4_8341_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30034335SMESG000009102.1 dd_Smed_v4_8341_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30034335SMESG000009102.1 dd_Smed_v4_8341_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30034335SMESG000009102.1 dd_Smed_v4_8341_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30034335SMESG000009102.1 dd_Smed_v4_8341_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30034335SMESG000080004.1 SMESG000050587.1 SMESG000009102.1 Contig48119newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30034335SMESG000080004.1 SMESG000050587.1 SMESG000009102.1 Contig48119uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
zeta neoblastSMED30034335SMESG000009102.1 dd_Smed_v4_8341_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30034335 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001852:2654..8915 -12840
Homology
BLAST of SMED30034335 vs. Planmine SMEST
Match: SMESG000009102.1 (SMESG000009102.1)

HSP 1 Score: 1535.01 bits (3973), Expect = 0.000e+0
Identity = 806/810 (99.51%), Postives = 809/810 (99.88%), Query Frame = -2
Query:  133 FFKIRTDLYFYFCLFTGMNVNPGLFANIVRSSLASLKHDEMYGDLIYGDDTGKYCCLNLCNSNFTLFQLPHLEFNANANYCAEVLAITPEAICAVKFTDDSVLRWSKSRPLSEEAKLLIETQQFWSWIPGSLCLVQLPHHLVESHKLTPVERAIIVSTGFATPLEVKQTKSNYLILHKTSFYLLDHKRYIPNVLCCSSSFQVIKRTAYKNNETNIGNVCNVSIRARCMPSKIQFVKLQNYNDVSQILRNKSGGFIPNKTLILYRCQQSEMSINFQPIFSCYSRINKKEFELNRIIEHYNEVVKSPPIRAIERVSGNKMRXXXXXXXXXXXXXXXXXXXXXXXDLTKSNSRGFRNSASSLEDEAVIRKGKTQPIGNKAVVNSDNSSINYSVKPDEDDRISVISFTDDEDVYDENXXXXXXXXXXXXXXXXXEQAEIPNDCVEEKRIDVIQPQEKLGKCIIPISSNLCSHFNERVVRILHLQSGAAVVILLESTALMKRELKMLSIMNQELSPIVNDISIGDYFIWCSNENIDFIKTVNNKTMALVEILQVINNKMALVSLIHYGLVVTVRRDDLRIIPKLNSVDDNFVDLIGPMIGAFDLHMIPNWYCAELDKSITDQLFTAFFINENELVLQYFNDPCKFYNLQLLSAIEHSLTNNHCNIRSKSFIAILSEALLNCRLFVGNLLVLSTAITEEFSIHSSLPISNTLVRIEEDSEPGVWFASVDIVNECGELVEKLSPHCFAEEKFCIDRVQIXXXXXXXXXXXNQNIYVDIPKYFQGIYKFEKKLLNEFTKSNGEFLCRYMLIGATEIPD 2562
            FFKIRTDLYFYFCLFTGMNVNPGLFANIVRSSLASLKHDEMYGDLIYGDDTGKYCCLNLCNSNFTLFQLPHLEFNANANYCAEVLAITPEAICAVKFTDDSVLRWSKSRPLSEEAKLLIETQQFWSWIPGSLCLVQLPHHLVESHKLTPVERAIIVSTGFATPLEVK+TKSNYLILHKTSFYLLDHKRYIPNVLCCSSSFQVIKRTAYK+NETNIGNVCNVSIRARCMPSKIQFVKLQNYNDVSQILRNKSGGFIPNKTLILYRCQQSEMSINFQPIFSCYSRINKKEFELNRIIEHYNEVVKSPPIRAIERVSGNKMRSDDIEVIVISDSDDDDDKEDSVIDLTKSNS GFRNSASSLEDEAVIRKGKTQPIGNKAV+NSDNSSINYSVKPDEDDRISVISFTDDEDVYDENELGEKESSVSSVSNENGEQAEIPNDCVEEKRIDVIQPQEKLGKCIIPISSNLCSHFNERVVRILHLQSGAAVVILLESTALMKRELKMLSIMNQELSPIVNDISIGDYFIWCSNENIDFIKTVNNKTMALVEILQVINNKMALVSLIHYGLVVTVRRDDLRIIPKLNSVDDNFVDLIGPMIGAFDLHMIPNWYCAELDKSITDQLFTAFFINENELVLQYFNDPCKFYNLQLLSAIEHSLTNNHCNIRSKSFIAILSEALLNCRLFVGNLLVLSTAITEEFSIHSSLPISNTLVRIEEDSEPGVWFASVDIVNECGELVEKLSPHCFAEEKFCIDRVQIDKKKNKLRLLDNQNIYVDIPKYFQGIYKFEKKLLNEFTKSNGEFLCRYMLIGATEIPD
Sbjct:  450 FFKIRTDLYFYFCLFTGMNVNPGLFANIVRSSLASLKHDEMYGDLIYGDDTGKYCCLNLCNSNFTLFQLPHLEFNANANYCAEVLAITPEAICAVKFTDDSVLRWSKSRPLSEEAKLLIETQQFWSWIPGSLCLVQLPHHLVESHKLTPVERAIIVSTGFATPLEVKKTKSNYLILHKTSFYLLDHKRYIPNVLCCSSSFQVIKRTAYKSNETNIGNVCNVSIRARCMPSKIQFVKLQNYNDVSQILRNKSGGFIPNKTLILYRCQQSEMSINFQPIFSCYSRINKKEFELNRIIEHYNEVVKSPPIRAIERVSGNKMRSDDIEVIVISDSDDDDDKEDSVIDLTKSNSLGFRNSASSLEDEAVIRKGKTQPIGNKAVINSDNSSINYSVKPDEDDRISVISFTDDEDVYDENELGEKESSVSSVSNENGEQAEIPNDCVEEKRIDVIQPQEKLGKCIIPISSNLCSHFNERVVRILHLQSGAAVVILLESTALMKRELKMLSIMNQELSPIVNDISIGDYFIWCSNENIDFIKTVNNKTMALVEILQVINNKMALVSLIHYGLVVTVRRDDLRIIPKLNSVDDNFVDLIGPMIGAFDLHMIPNWYCAELDKSITDQLFTAFFINENELVLQYFNDPCKFYNLQLLSAIEHSLTNNHCNIRSKSFIAILSEALLNCRLFVGNLLVLSTAITEEFSIHSSLPISNTLVRIEEDSEPGVWFASVDIVNECGELVEKLSPHCFAEEKFCIDRVQIDKKKNKLRLLDNQNIYVDIPKYFQGIYKFEKKLLNEFTKSNGEFLCRYMLIGATEIPD 1259          

HSP 2 Score: 367.081 bits (941), Expect = 5.655e-108
Identity = 239/242 (98.76%), Postives = 241/242 (99.59%), Query Frame = -1
Query: 2573 MTLYDTVVSNLKGVIGSNKDGLSLNSITSEYEFFAGDSFPISQLGYDSILSFLKSVPHLFKIKYLGNATRVYNVIGKNESESNLVLYDMIQNTTAKKGPKFRRMPFLSNHKPIIXXXXXXXXXXXXXXYLKHPNGPNGFATTGFNSLGSNNASKNNLQAFENFQIQVENNLIKNNHLKSNDEFYXXXXXXXXXXXXEPVSIGXXXXXXXXXXXXXXXXXXXXXKFVDILIEIIVEFKPLNLD 3298
            +TLYDTVVSNLKGVIGSNKDGLSLNSITSEYEFFAGDSFPISQLGYDSILSFLKSVPHLFKIKYLGNATRVYNVIGKNESESNLVLYDMIQNTTAKKGPKFRRMPFLSNHKP INNGFGSNSNFSYSNYLKHPNGPNGFATTGFNSLGSNNASKNNLQAFENFQIQVENNLIKNNHLKSNDEFYLSDSESSDSNLNEPVSIGIQTEEIIYKKITTT+STLKTSKFVDILIEIIVEFKPLNLD
Sbjct:  205 LTLYDTVVSNLKGVIGSNKDGLSLNSITSEYEFFAGDSFPISQLGYDSILSFLKSVPHLFKIKYLGNATRVYNVIGKNESESNLVLYDMIQNTTAKKGPKFRRMPFLSNHKPTINNGFGSNSNFSYSNYLKHPNGPNGFATTGFNSLGSNNASKNNLQAFENFQIQVENNLIKNNHLKSNDEFYLSDSESSDSNLNEPVSIGIQTEEIIYKKITTTISTLKTSKFVDILIEIIVEFKPLNLD 446          
BLAST of SMED30034335 vs. Planmine SMEST
Match: SMESG000009102.1 (SMESG000009102.1)

HSP 1 Score: 1534.24 bits (3971), Expect = 0.000e+0
Identity = 806/810 (99.51%), Postives = 809/810 (99.88%), Query Frame = -2
Query:  133 FFKIRTDLYFYFCLFTGMNVNPGLFANIVRSSLASLKHDEMYGDLIYGDDTGKYCCLNLCNSNFTLFQLPHLEFNANANYCAEVLAITPEAICAVKFTDDSVLRWSKSRPLSEEAKLLIETQQFWSWIPGSLCLVQLPHHLVESHKLTPVERAIIVSTGFATPLEVKQTKSNYLILHKTSFYLLDHKRYIPNVLCCSSSFQVIKRTAYKNNETNIGNVCNVSIRARCMPSKIQFVKLQNYNDVSQILRNKSGGFIPNKTLILYRCQQSEMSINFQPIFSCYSRINKKEFELNRIIEHYNEVVKSPPIRAIERVSGNKMRXXXXXXXXXXXXXXXXXXXXXXXDLTKSNSRGFRNSASSLEDEAVIRKGKTQPIGNKAVVNSDNSSINYSVKPDEDDRISVISFTDDEDVYDENXXXXXXXXXXXXXXXXXEQAEIPNDCVEEKRIDVIQPQEKLGKCIIPISSNLCSHFNERVVRILHLQSGAAVVILLESTALMKRELKMLSIMNQELSPIVNDISIGDYFIWCSNENIDFIKTVNNKTMALVEILQVINNKMALVSLIHYGLVVTVRRDDLRIIPKLNSVDDNFVDLIGPMIGAFDLHMIPNWYCAELDKSITDQLFTAFFINENELVLQYFNDPCKFYNLQLLSAIEHSLTNNHCNIRSKSFIAILSEALLNCRLFVGNLLVLSTAITEEFSIHSSLPISNTLVRIEEDSEPGVWFASVDIVNECGELVEKLSPHCFAEEKFCIDRVQIXXXXXXXXXXXNQNIYVDIPKYFQGIYKFEKKLLNEFTKSNGEFLCRYMLIGATEIPD 2562
            FFKIRTDLYFYFCLFTGMNVNPGLFANIVRSSLASLKHDEMYGDLIYGDDTGKYCCLNLCNSNFTLFQLPHLEFNANANYCAEVLAITPEAICAVKFTDDSVLRWSKSRPLSEEAKLLIETQQFWSWIPGSLCLVQLPHHLVESHKLTPVERAIIVSTGFATPLEVK+TKSNYLILHKTSFYLLDHKRYIPNVLCCSSSFQVIKRTAYK+NETNIGNVCNVSIRARCMPSKIQFVKLQNYNDVSQILRNKSGGFIPNKTLILYRCQQSEMSINFQPIFSCYSRINKKEFELNRIIEHYNEVVKSPPIRAIERVSGNKMRSDDIEVIVISDSDDDDDKEDSVIDLTKSNS GFRNSASSLEDEAVIRKGKTQPIGNKAV+NSDNSSINYSVKPDEDDRISVISFTDDEDVYDENELGEKESSVSSVSNENGEQAEIPNDCVEEKRIDVIQPQEKLGKCIIPISSNLCSHFNERVVRILHLQSGAAVVILLESTALMKRELKMLSIMNQELSPIVNDISIGDYFIWCSNENIDFIKTVNNKTMALVEILQVINNKMALVSLIHYGLVVTVRRDDLRIIPKLNSVDDNFVDLIGPMIGAFDLHMIPNWYCAELDKSITDQLFTAFFINENELVLQYFNDPCKFYNLQLLSAIEHSLTNNHCNIRSKSFIAILSEALLNCRLFVGNLLVLSTAITEEFSIHSSLPISNTLVRIEEDSEPGVWFASVDIVNECGELVEKLSPHCFAEEKFCIDRVQIDKKKNKLRLLDNQNIYVDIPKYFQGIYKFEKKLLNEFTKSNGEFLCRYMLIGATEIPD
Sbjct:  459 FFKIRTDLYFYFCLFTGMNVNPGLFANIVRSSLASLKHDEMYGDLIYGDDTGKYCCLNLCNSNFTLFQLPHLEFNANANYCAEVLAITPEAICAVKFTDDSVLRWSKSRPLSEEAKLLIETQQFWSWIPGSLCLVQLPHHLVESHKLTPVERAIIVSTGFATPLEVKKTKSNYLILHKTSFYLLDHKRYIPNVLCCSSSFQVIKRTAYKSNETNIGNVCNVSIRARCMPSKIQFVKLQNYNDVSQILRNKSGGFIPNKTLILYRCQQSEMSINFQPIFSCYSRINKKEFELNRIIEHYNEVVKSPPIRAIERVSGNKMRSDDIEVIVISDSDDDDDKEDSVIDLTKSNSLGFRNSASSLEDEAVIRKGKTQPIGNKAVINSDNSSINYSVKPDEDDRISVISFTDDEDVYDENELGEKESSVSSVSNENGEQAEIPNDCVEEKRIDVIQPQEKLGKCIIPISSNLCSHFNERVVRILHLQSGAAVVILLESTALMKRELKMLSIMNQELSPIVNDISIGDYFIWCSNENIDFIKTVNNKTMALVEILQVINNKMALVSLIHYGLVVTVRRDDLRIIPKLNSVDDNFVDLIGPMIGAFDLHMIPNWYCAELDKSITDQLFTAFFINENELVLQYFNDPCKFYNLQLLSAIEHSLTNNHCNIRSKSFIAILSEALLNCRLFVGNLLVLSTAITEEFSIHSSLPISNTLVRIEEDSEPGVWFASVDIVNECGELVEKLSPHCFAEEKFCIDRVQIDKKKNKLRLLDNQNIYVDIPKYFQGIYKFEKKLLNEFTKSNGEFLCRYMLIGATEIPD 1268          

HSP 2 Score: 366.311 bits (939), Expect = 1.191e-107
Identity = 239/241 (99.17%), Postives = 240/241 (99.59%), Query Frame = -1
Query: 2573 TLYDTVVSNLKGVIGSNKDGLSLNSITSEYEFFAGDSFPISQLGYDSILSFLKSVPHLFKIKYLGNATRVYNVIGKNESESNLVLYDMIQNTTAKKGPKFRRMPFLSNHKPIIXXXXXXXXXXXXXXYLKHPNGPNGFATTGFNSLGSNNASKNNLQAFENFQIQVENNLIKNNHLKSNDEFYXXXXXXXXXXXXEPVSIGXXXXXXXXXXXXXXXXXXXXXKFVDILIEIIVEFKPLNLD 3295
            TLYDTVVSNLKGVIGSNKDGLSLNSITSEYEFFAGDSFPISQLGYDSILSFLKSVPHLFKIKYLGNATRVYNVIGKNESESNLVLYDMIQNTTAKKGPKFRRMPFLSNHKP INNGFGSNSNFSYSNYLKHPNGPNGFATTGFNSLGSNNASKNNLQAFENFQIQVENNLIKNNHLKSNDEFYLSDSESSDSNLNEPVSIGIQTEEIIYKKITTT+STLKTSKFVDILIEIIVEFKPLNLD
Sbjct:  215 TLYDTVVSNLKGVIGSNKDGLSLNSITSEYEFFAGDSFPISQLGYDSILSFLKSVPHLFKIKYLGNATRVYNVIGKNESESNLVLYDMIQNTTAKKGPKFRRMPFLSNHKPTINNGFGSNSNFSYSNYLKHPNGPNGFATTGFNSLGSNNASKNNLQAFENFQIQVENNLIKNNHLKSNDEFYLSDSESSDSNLNEPVSIGIQTEEIIYKKITTTISTLKTSKFVDILIEIIVEFKPLNLD 455          
The following BLAST results are available for this feature:
BLAST of SMED30034335 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30034335 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30034335 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30034335 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30034335 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30034335 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30034335 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30034335 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30034335 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30034335 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30034335 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30034335 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30034335 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30034335 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000009102.15.655e-10899.51SMESG000009102.1[more]
SMESG000009102.11.191e-10799.51SMESG000009102.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034335 ID=SMED30034335|Name=SMED30034335|organism=Schmidtea mediterranea sexual|type=transcript|length=3316bp
CAGAGTGACAATATTTATTATAAAAATGCTATTATAAATGCAGATTATTA
ATCATAAACAAACAAATTACATAACACAAATCTGAAAAATGTTAACCAAT
CAAAATATTAATTTGTTGAACAAAAAATTTAAATCTGGAATCTCTGTTGC
TCCTATTAACATATATCGACAGAGAAATTCCCCATTAGATTTTGTAAATT
CATTAAGTAATTTTTTTTCGAATTTATATATTCCCTGGAAATATTTGGGA
ATGTCGACGTAAATATTTTGATTGTCCAATAGGCGCAGCTTATTCTTTTT
CTTATCGATTTGAACCCGATCTATACAGAATTTTTCTTCCGCAAAACAAT
GAGGTGACAATTTTTCAACCAATTCACCACATTCATTTACAATATCAACT
GAAGCGAACCAAACTCCAGGTTCACTGTCTTCTTCAATGCGTACCAATGT
ATTTGATATTGGCAGGGATGAATGTATCGAAAATTCTTCGGTAATTGCTG
TAGAGAGTACCAGTAAATTTCCGACAAATAATCGACAATTCAGTAAAGCC
TCAGACAAAATAGCAATGAACGATTTGGACCGAATATTGCAATGATTATT
TGTGAGAGAATGTTCGATTGCCGATAGCAATTGTAAATTATAAAACTTAC
ATGGATCATTAAAATATTGCAAGACTAATTCATTTTCATTAATAAAAAAT
GCAGTAAATAACTGATCGGTTATGGATTTATCCAATTCAGCACAATACCA
ATTGGGTATCATGTGTAAGTCGAACGCACCAATCATTGGACCAATTAAAT
CCACAAAATTATCATCTACACTGTTAAGCTTGGGAATTATGCGTAAGTCA
TCGCGCCTCACTGTGACGACCAGACCATAATGTATCAATGAAACTAGAGC
CATTTTATTATTTATAACTTGTAGAATTTCAACAAGAGCCATAGTTTTGT
TATTTACTGTTTTGATAAAATCGATATTTTCATTGCTACACCATATGAAG
TAGTCACCAATGGAAATGTCATTAACAATAGGAGAAAGCTCTTGATTCAT
TATTGACAGCATTTTCAGTTCCCGTTTCATTAAGGCCGTCGATTCGAGTA
GAATGACAACAGCGGCTCCGGATTGTAAATGCAAAATTCTCACAACTCTT
TCATTAAAATGACTGCAAAGATTCGAAGAAATCGGTATAATACATTTTCC
TAGTTTCTCTTGAGGCTGAATTACATCAATACGCTTCTCTTCGACACAAT
CATTAGGAATTTCTGCCTGCTCTCCATTCTCGTTGGAAACACTGGAAACT
GACGACTCCTTTTCTCCCAATTCATTCTCATCATAAACATCCTCATCATC
CGTGAATGATATTACAGAAATCCTGTCATCCTCGTCTGGCTTTACAGAGT
AGTTAATCGATGAATTGTCGGAATTTACAACTGCCTTATTTCCGATTGGC
TGCGTTTTGCCCTTTCTGATAACCGCTTCATCTTCCAATGAACTAGCTGA
ATTCCGGAATCCTCGACTGTTTGACTTTGTCAAATCGATTACTGAATCCT
CTTTATCGTCGTCATCATCACTGTCCGATATTACGATTACTTCAATATCA
TCACTTCTCATTTTATTCCCGGAAACCCTTTCAATCGCTCTGATTGGTGG
CGATTTCACCACCTCATTGTAATGTTCAATGATTCGATTCAACTCGAATT
CTTTCTTATTGATTCGCGAATAACAACTGAAAATCGGTTGAAAATTTATA
GACATTTCCGATTGTTGGCAACGATATAAAATCAGCGTTTTATTCGGAAT
AAATCCACCGGATTTATTGCGTAATATCTGTGAGACGTCGTTGTAGTTTT
GAAGTTTTACAAATTGGATTTTCGACGGCATGCAACGGGCACGAATCGAT
ACGTTGCAAACATTGCCGATATTGGTTTCATTGTTTTTATAGGCAGTCCG
CTTGATCACTTGAAAGCTGCTACTGCAACAGAGGACATTCGGTATGTAGC
GTTTATGGTCGAGGAGATAGAATGAGGTTTTGTGGAGAATTAGATAGTTG
GACTTGGTCTGTTTAACTTCGAGAGGAGTGGCGAACCCCGTGGAAACGAT
TATAGCTCTTTCGACGGGGGTCAGTTTATGAGATTCCACCAAATGATGCG
GTAATTGGACAAGGCACAAGCTGCCGGGTATCCACGACCAGAATTGCTGG
GTTTCAATCAATAATTTGGCTTCCTCAGAAAGCGGCCGACTTTTACTCCA
TCTTAATACGGAATCGTCGGTAAATTTTACGGCACAAATCGCCTCAGGGG
TGATAGCAAGCACCTCGGCACAATAATTCGCATTGGCGTTGAATTCCAGA
TGGGGCAGTTGGAACAGGGTGAAATTGGAATTGCAAAGGTTCAGGCAGCA
ATATTTACCGGTGTCATCGCCGTAAATAAGATCCCCATACATTTCATCGT
GTTTCAACGATGCAAGAGAGCTGCGGACGATATTAGCAAATAATCCCGGA
TTCACATTCATACCGGTGAATAGGCAAAAGTAAAAATACAAATCGGTTCT
GATTTTAAAAAAAGACAACATTGTCAAGATTTAACGGCTTGAATTCGACA
ATTATTTCGATTAGAATGTCTACGAATTTTGAAGTCTTTAAAGTTGAAAG
GGTAGTAGTAATTTTTTTATATATGATTTCTTCGGTTTGTATTCCTATTG
AAACAGGTTCGTTGAGATTTGAATCGCTGCTTTCACTGTCACTTAAATAG
AACTCATCGTTACTTTTGAGATGGTTGTTTTTGATAAGATTATTTTCTAC
TTGAATTTGAAAGTTTTCAAACGCTTGTAAGTTATTTTTAGATGCATTAT
TTGATCCGAGACTATTAAAACCCGTGGTCGCAAAACCATTGGGACCATTA
GGATGTTTTAAATAATTAGAATAAGAAAAGTTTGAGTTAGATCCAAAACC
ATTGTTAATTATCGGTTTGTGATTTGATAGAAATGGCATTCGTCTAAATT
TTGGTCCTTTCTTGGCTGTTGTATTTTGTATCATGTCATATAAAACTAAA
TTTGATTCGCTTTCATTTTTTCCAATTACATTGTATACTCGTGTTGCATT
GCCTAAATATTTTATTTTAAACAAATGAGGAACTGATTTGAGAAAGGATA
AAATTGAATCATATCCTAATTGGCTTATTGGAAAAGAATCTCCTGCAAAA
AACTCGTATTCGGAAGTAATAGAATTTAGGGATAACCCATCTTTATTTGA
ACCTATCACTCCTTTTAAATTTGAAACTACAGTATCGTAGAGAGTCATAT
AAGATTTATATAGATT
back to top

protein sequence of SMED30034335-orf-1

>SMED30034335-orf-1 ID=SMED30034335-orf-1|Name=SMED30034335-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=254bp
NLYKSYMTLYDTVVSNLKGVIGSNKDGLSLNSITSEYEFFAGDSFPISQL
GYDSILSFLKSVPHLFKIKYLGNATRVYNVIGKNESESNLVLYDMIQNTT
AKKGPKFRRMPFLSNHKPIINNGFGSNSNFSYSNYLKHPNGPNGFATTGF
NSLGSNNASKNNLQAFENFQIQVENNLIKNNHLKSNDEFYLSDSESSDSN
LNEPVSIGIQTEEIIYKKITTTLSTLKTSKFVDILIEIIVEFKPLNLDNV
VFF*
back to top

protein sequence of SMED30034335-orf-2

>SMED30034335-orf-2 ID=SMED30034335-orf-2|Name=SMED30034335-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=824bp
MLSFFKIRTDLYFYFCLFTGMNVNPGLFANIVRSSLASLKHDEMYGDLIY
GDDTGKYCCLNLCNSNFTLFQLPHLEFNANANYCAEVLAITPEAICAVKF
TDDSVLRWSKSRPLSEEAKLLIETQQFWSWIPGSLCLVQLPHHLVESHKL
TPVERAIIVSTGFATPLEVKQTKSNYLILHKTSFYLLDHKRYIPNVLCCS
SSFQVIKRTAYKNNETNIGNVCNVSIRARCMPSKIQFVKLQNYNDVSQIL
RNKSGGFIPNKTLILYRCQQSEMSINFQPIFSCYSRINKKEFELNRIIEH
YNEVVKSPPIRAIERVSGNKMRSDDIEVIVISDSDDDDDKEDSVIDLTKS
NSRGFRNSASSLEDEAVIRKGKTQPIGNKAVVNSDNSSINYSVKPDEDDR
ISVISFTDDEDVYDENELGEKESSVSSVSNENGEQAEIPNDCVEEKRIDV
IQPQEKLGKCIIPISSNLCSHFNERVVRILHLQSGAAVVILLESTALMKR
ELKMLSIMNQELSPIVNDISIGDYFIWCSNENIDFIKTVNNKTMALVEIL
QVINNKMALVSLIHYGLVVTVRRDDLRIIPKLNSVDDNFVDLIGPMIGAF
DLHMIPNWYCAELDKSITDQLFTAFFINENELVLQYFNDPCKFYNLQLLS
AIEHSLTNNHCNIRSKSFIAILSEALLNCRLFVGNLLVLSTAITEEFSIH
SSLPISNTLVRIEEDSEPGVWFASVDIVNECGELVEKLSPHCFAEEKFCI
DRVQIDKKKNKLRLLDNQNIYVDIPKYFQGIYKFEKKLLNEFTKSNGEFL
CRYMLIGATEIPDLNFLFNKLIF*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000020protonephridia
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR025605OST-HTH/LOTUS_dom