Eukaryotic translation initiation factor 5A

Overview
NameEukaryotic translation initiation factor 5A
Smed IDSMED30034271
Length (bp)887
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Eukaryotic translation initiation factor 5A (SMED30034271) t-SNE clustered cells

Violin plots show distribution of expression levels for Eukaryotic translation initiation factor 5A (SMED30034271) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Eukaryotic translation initiation factor 5A (SMED30034271) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Eukaryotic translation initiation factor 5A (SMED30034271) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30034271

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30034271h1SMcG0004607 Contig986GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30034271h1SMcG0004606 Contig986GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30034271h1SMcG0021903 Contig986GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30034271h1SMcG0021903 Contig51779uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30034271h1SMcG0021903 Contig51779newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30034271h1SMcG0021903 dd_Smed_v4_484_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30034271h1SMcG0021903 dd_Smed_v4_484_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30034271h1SMcG0021903 dd_Smed_v4_484_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30034271h1SMcG0021903 dd_Smed_v4_484_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30034271h1SMcG0021903 dd_Smed_v4_484_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30034271h1SMcG0021903 dd_Smed_v4_484_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30034271h1SMcG0021903 dd_Smed_v4_484_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30034271h1SMcG0021903 dd_Smed_v6_484_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Human
Match: EIF5A (eukaryotic translation initiation factor 5A [Source:HGNC Symbol;Acc:HGNC:3300])

HSP 1 Score: 157.918 bits (398), Expect = 1.448e-47
Identity = 71/145 (48.97%), Postives = 109/145 (75.17%), Query Frame = 1 
Query:   34 FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            FE  D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT +K ED+ PST N D+P +KR ++QL+ I  DGY+SL+ + G+V+ED++LP G+L  +++ +++ G+ +++TV+SA +EE  V+ +
Sbjct:    7 FETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQ-DGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Human
Match: EIF5A (eukaryotic translation initiation factor 5A [Source:HGNC Symbol;Acc:HGNC:3300])

HSP 1 Score: 157.918 bits (398), Expect = 1.448e-47
Identity = 71/145 (48.97%), Postives = 109/145 (75.17%), Query Frame = 1 
Query:   34 FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            FE  D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT +K ED+ PST N D+P +KR ++QL+ I  DGY+SL+ + G+V+ED++LP G+L  +++ +++ G+ +++TV+SA +EE  V+ +
Sbjct:    7 FETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQ-DGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Human
Match: EIF5A (eukaryotic translation initiation factor 5A [Source:HGNC Symbol;Acc:HGNC:3300])

HSP 1 Score: 157.918 bits (398), Expect = 1.448e-47
Identity = 71/145 (48.97%), Postives = 109/145 (75.17%), Query Frame = 1 
Query:   34 FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            FE  D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT +K ED+ PST N D+P +KR ++QL+ I  DGY+SL+ + G+V+ED++LP G+L  +++ +++ G+ +++TV+SA +EE  V+ +
Sbjct:    7 FETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQ-DGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Human
Match: EIF5A (eukaryotic translation initiation factor 5A [Source:HGNC Symbol;Acc:HGNC:3300])

HSP 1 Score: 157.918 bits (398), Expect = 1.448e-47
Identity = 71/145 (48.97%), Postives = 109/145 (75.17%), Query Frame = 1 
Query:   34 FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            FE  D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT +K ED+ PST N D+P +KR ++QL+ I  DGY+SL+ + G+V+ED++LP G+L  +++ +++ G+ +++TV+SA +EE  V+ +
Sbjct:    7 FETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQ-DGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Human
Match: EIF5A (eukaryotic translation initiation factor 5A [Source:HGNC Symbol;Acc:HGNC:3300])

HSP 1 Score: 157.918 bits (398), Expect = 1.448e-47
Identity = 71/145 (48.97%), Postives = 109/145 (75.17%), Query Frame = 1 
Query:   34 FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            FE  D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT +K ED+ PST N D+P +KR ++QL+ I  DGY+SL+ + G+V+ED++LP G+L  +++ +++ G+ +++TV+SA +EE  V+ +
Sbjct:    7 FETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQ-DGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Celegans
Match: iff-1 (Eukaryotic translation initiation factor 5A-1 [Source:UniProtKB/Swiss-Prot;Acc:P34563])

HSP 1 Score: 147.132 bits (370), Expect = 8.579e-44
Identity = 73/147 (49.66%), Postives = 106/147 (72.11%), Query Frame = 1 
Query:   25 EDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLM-SEKGDVKEDVQLPGGELEADLKTRFENGD-MVMVTVMSACSEEKIV 459
            E+ F++ +SGA+ T P Q SA RK +++MI+ RPCKI++MST KTGKHG AKVH+ A+DIFT +K ED+ PST N D+P+VKR EY L+ I  DG+ SLM  E  ++K+D+++P G+L   ++   E  +  V+V V++AC EE I+
Sbjct:    8 EEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAIDIFTTKKLEDICPSTHNMDVPVVKRREYILMSIE-DGFCSLMDPESCELKDDLKMPEGDLGNTIREALEKDEGSVLVQVVAACGEEAIL 153
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Celegans
Match: iff-1 (Eukaryotic translation initiation factor 5A-1 [Source:UniProtKB/Swiss-Prot;Acc:P34563])

HSP 1 Score: 147.132 bits (370), Expect = 8.579e-44
Identity = 73/147 (49.66%), Postives = 106/147 (72.11%), Query Frame = 1 
Query:   25 EDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLM-SEKGDVKEDVQLPGGELEADLKTRFENGD-MVMVTVMSACSEEKIV 459
            E+ F++ +SGA+ T P Q SA RK +++MI+ RPCKI++MST KTGKHG AKVH+ A+DIFT +K ED+ PST N D+P+VKR EY L+ I  DG+ SLM  E  ++K+D+++P G+L   ++   E  +  V+V V++AC EE I+
Sbjct:    8 EEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAIDIFTTKKLEDICPSTHNMDVPVVKRREYILMSIE-DGFCSLMDPESCELKDDLKMPEGDLGNTIREALEKDEGSVLVQVVAACGEEAIL 153
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Celegans
Match: iff-1 (Eukaryotic translation initiation factor 5A-1 [Source:UniProtKB/Swiss-Prot;Acc:P34563])

HSP 1 Score: 147.517 bits (371), Expect = 1.847e-43
Identity = 73/147 (49.66%), Postives = 106/147 (72.11%), Query Frame = 1 
Query:   25 EDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLM-SEKGDVKEDVQLPGGELEADLKTRFENGD-MVMVTVMSACSEEKIV 459
            E+ F++ +SGA+ T P Q SA RK +++MI+ RPCKI++MST KTGKHG AKVH+ A+DIFT +K ED+ PST N D+P+VKR EY L+ I  DG+ SLM  E  ++K+D+++P G+L   ++   E  +  V+V V++AC EE I+
Sbjct:   42 EEQFDSAESGAAATFPKQCSALRKNEHVMIRGRPCKIVEMSTSKTGKHGHAKVHMVAIDIFTTKKLEDICPSTHNMDVPVVKRREYILMSIE-DGFCSLMDPESCELKDDLKMPEGDLGNTIREALEKDEGSVLVQVVAACGEEAIL 187
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Celegans
Match: iff-2 (Eukaryotic translation initiation factor 5A-2 [Source:UniProtKB/Swiss-Prot;Acc:Q20751])

HSP 1 Score: 142.895 bits (359), Expect = 4.353e-42
Identity = 75/143 (52.45%), Postives = 100/143 (69.93%), Query Frame = 1 
Query:   46 DSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLM-SEKGDVKEDVQLPGGELEADLKTRFENGD-MVMVTVMSACSEEKIVSCR 468
            DSGA+ T P Q SA RK +++MIK RPCKI++MST KTGKHG AKVH+ A+DIFT +K ED+ PST N D+P+VKR EY L+ I  DGY SLM  E  + K+D++LP  EL   ++  +E  +  V+V V+SA  EE I+  +
Sbjct:   15 DSGAAATFPKQCSALRKNEHVMIKGRPCKIVEMSTSKTGKHGHAKVHMVAIDIFTSKKLEDICPSTHNMDVPVVKRREYLLMAID-DGYCSLMDPESCEQKDDLKLPDTELGQQIRDAYEKDEGSVLVQVVSAIGEEAILGWK 156
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Celegans
Match: iff-2 (Eukaryotic translation initiation factor 5A-2 [Source:UniProtKB/Swiss-Prot;Acc:Q20751])

HSP 1 Score: 142.895 bits (359), Expect = 4.353e-42
Identity = 75/143 (52.45%), Postives = 100/143 (69.93%), Query Frame = 1 
Query:   46 DSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLM-SEKGDVKEDVQLPGGELEADLKTRFENGD-MVMVTVMSACSEEKIVSCR 468
            DSGA+ T P Q SA RK +++MIK RPCKI++MST KTGKHG AKVH+ A+DIFT +K ED+ PST N D+P+VKR EY L+ I  DGY SLM  E  + K+D++LP  EL   ++  +E  +  V+V V+SA  EE I+  +
Sbjct:   15 DSGAAATFPKQCSALRKNEHVMIKGRPCKIVEMSTSKTGKHGHAKVHMVAIDIFTSKKLEDICPSTHNMDVPVVKRREYLLMAID-DGYCSLMDPESCEQKDDLKLPDTELGQQIRDAYEKDEGSVLVQVVSAIGEEAILGWK 156
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Fly
Match: eEF5 (gene:FBgn0285952 transcript:FBtr0072172)

HSP 1 Score: 171.4 bits (433), Expect = 3.832e-53
Identity = 78/155 (50.32%), Postives = 113/155 (72.90%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVT 477
            M+E +D FE  DSGAS T P Q SA RK  ++M+K RPCKI++MST KTGKHG AKVH+  +DIF+++K ED+ PST N D+P VKR + QL+ IS D +++LM+E GD++ED+++P GEL   L+  F++G  ++ TV+ AC EE +++ +  T
Sbjct:    1 MAELDDQFETTDSGASTTYPMQCSALRKNGFVMLKSRPCKIVEMSTSKTGKHGHAKVHMVGIDIFSNKKYEDICPSTHNMDVPNVKREDLQLIAISDDSFLTLMTESGDLREDLKVPEGELGEQLRLDFDSGKDLLCTVLKACGEECVIAIKTNT 155
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Fly
Match: eEF5 (gene:FBgn0285952 transcript:FBtr0072173)

HSP 1 Score: 171.4 bits (433), Expect = 3.832e-53
Identity = 78/155 (50.32%), Postives = 113/155 (72.90%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVT 477
            M+E +D FE  DSGAS T P Q SA RK  ++M+K RPCKI++MST KTGKHG AKVH+  +DIF+++K ED+ PST N D+P VKR + QL+ IS D +++LM+E GD++ED+++P GEL   L+  F++G  ++ TV+ AC EE +++ +  T
Sbjct:    1 MAELDDQFETTDSGASTTYPMQCSALRKNGFVMLKSRPCKIVEMSTSKTGKHGHAKVHMVGIDIFSNKKYEDICPSTHNMDVPNVKREDLQLIAISDDSFLTLMTESGDLREDLKVPEGELGEQLRLDFDSGKDLLCTVLKACGEECVIAIKTNT 155
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Zebrafish
Match: eif5a (eukaryotic translation initiation factor 5A [Source:ZFIN;Acc:ZDB-GENE-040426-2229])

HSP 1 Score: 162.925 bits (411), Expect = 8.011e-50
Identity = 77/155 (49.68%), Postives = 116/155 (74.84%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVT 477
            M++ +  F + D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT++K ED+ PST N D+P +KR +YQLVDIS +G++SLM + GDV+ED+++P G+L  +++ +F  G+ ++VTV+SA  EE  V+ + +T
Sbjct:    1 MADPDLDFASGDAGASATYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTNKKYEDICPSTHNMDVPNIKRNDYQLVDIS-EGFLSLMMDNGDVREDLRVPDGDLGKEIENKFAAGEEMLVTVLSAMGEESAVALKPMT 154
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Zebrafish
Match: eif5a (eukaryotic translation initiation factor 5A [Source:ZFIN;Acc:ZDB-GENE-040426-2229])

HSP 1 Score: 162.925 bits (411), Expect = 8.011e-50
Identity = 77/155 (49.68%), Postives = 116/155 (74.84%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVT 477
            M++ +  F + D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT++K ED+ PST N D+P +KR +YQLVDIS +G++SLM + GDV+ED+++P G+L  +++ +F  G+ ++VTV+SA  EE  V+ + +T
Sbjct:    1 MADPDLDFASGDAGASATYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTNKKYEDICPSTHNMDVPNIKRNDYQLVDIS-EGFLSLMMDNGDVREDLRVPDGDLGKEIENKFAAGEEMLVTVLSAMGEESAVALKPMT 154
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Zebrafish
Match: eif5a (eukaryotic translation initiation factor 5A [Source:ZFIN;Acc:ZDB-GENE-040426-2229])

HSP 1 Score: 162.925 bits (411), Expect = 8.011e-50
Identity = 77/155 (49.68%), Postives = 116/155 (74.84%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVT 477
            M++ +  F + D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT++K ED+ PST N D+P +KR +YQLVDIS +G++SLM + GDV+ED+++P G+L  +++ +F  G+ ++VTV+SA  EE  V+ + +T
Sbjct:    1 MADPDLDFASGDAGASATYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTNKKYEDICPSTHNMDVPNIKRNDYQLVDIS-EGFLSLMMDNGDVREDLRVPDGDLGKEIENKFAAGEEMLVTVLSAMGEESAVALKPMT 154
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Zebrafish
Match: eif5a (eukaryotic translation initiation factor 5A [Source:ZFIN;Acc:ZDB-GENE-040426-2229])

HSP 1 Score: 162.925 bits (411), Expect = 8.011e-50
Identity = 77/155 (49.68%), Postives = 116/155 (74.84%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVT 477
            M++ +  F + D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT++K ED+ PST N D+P +KR +YQLVDIS +G++SLM + GDV+ED+++P G+L  +++ +F  G+ ++VTV+SA  EE  V+ + +T
Sbjct:    1 MADPDLDFASGDAGASATYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTNKKYEDICPSTHNMDVPNIKRNDYQLVDIS-EGFLSLMMDNGDVREDLRVPDGDLGKEIENKFAAGEEMLVTVLSAMGEESAVALKPMT 154
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Zebrafish
Match: eif5a (eukaryotic translation initiation factor 5A [Source:ZFIN;Acc:ZDB-GENE-040426-2229])

HSP 1 Score: 162.925 bits (411), Expect = 8.011e-50
Identity = 77/155 (49.68%), Postives = 116/155 (74.84%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVT 477
            M++ +  F + D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT++K ED+ PST N D+P +KR +YQLVDIS +G++SLM + GDV+ED+++P G+L  +++ +F  G+ ++VTV+SA  EE  V+ + +T
Sbjct:    1 MADPDLDFASGDAGASATYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTNKKYEDICPSTHNMDVPNIKRNDYQLVDIS-EGFLSLMMDNGDVREDLRVPDGDLGKEIENKFAAGEEMLVTVLSAMGEESAVALKPMT 154
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Xenopus
Match: ddo (D-aspartate oxidase [Source:Xenbase;Acc:XB-GENE-6258233])

HSP 1 Score: 154.451 bits (389), Expect = 2.501e-46
Identity = 72/148 (48.65%), Postives = 110/148 (74.32%), Query Frame = 1 
Query:   34 FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVT 477
            F + D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT +K ED+ PST N D+P +KR +YQL+ I+ D Y+SL+S+ GDV+ED+++P G+L  ++  + + G+ ++VTV++A +EE  V+ + +T
Sbjct:    7 FTSGDAGASSTFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRCDYQLIGIN-DNYLSLLSDSGDVREDLKMPEGDLGREILMKHDGGEEILVTVLTAMNEECAVALKAMT 153
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Xenopus
Match: ddo (D-aspartate oxidase [Source:Xenbase;Acc:XB-GENE-6258233])

HSP 1 Score: 143.665 bits (361), Expect = 3.885e-42
Identity = 65/133 (48.87%), Postives = 99/133 (74.44%), Query Frame = 1 
Query:   34 FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVM 432
            F + D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT +K ED+ PST N D+P +KR +YQL+ I+ D Y+SL+S+ GDV+ED+++P G+L  ++  + + G+ ++V ++
Sbjct:    7 FTSGDAGASSTFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRCDYQLIGIN-DNYLSLLSDSGDVREDLKMPEGDLGREILMKHDGGEEILVNLL 138
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Mouse
Match: Eif5a (eukaryotic translation initiation factor 5A [Source:MGI Symbol;Acc:MGI:106248])

HSP 1 Score: 157.918 bits (398), Expect = 9.942e-48
Identity = 71/145 (48.97%), Postives = 109/145 (75.17%), Query Frame = 1 
Query:   34 FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            FE  D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT +K ED+ PST N D+P +KR ++QL+ I  DGY+SL+ + G+V+ED++LP G+L  +++ +++ G+ +++TV+SA +EE  V+ +
Sbjct:    7 FETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQ-DGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Mouse
Match: Eif5a (eukaryotic translation initiation factor 5A [Source:MGI Symbol;Acc:MGI:106248])

HSP 1 Score: 157.918 bits (398), Expect = 9.942e-48
Identity = 71/145 (48.97%), Postives = 109/145 (75.17%), Query Frame = 1 
Query:   34 FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            FE  D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT +K ED+ PST N D+P +KR ++QL+ I  DGY+SL+ + G+V+ED++LP G+L  +++ +++ G+ +++TV+SA +EE  V+ +
Sbjct:    7 FETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQ-DGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Mouse
Match: Eif5a (eukaryotic translation initiation factor 5A [Source:MGI Symbol;Acc:MGI:106248])

HSP 1 Score: 157.918 bits (398), Expect = 9.942e-48
Identity = 71/145 (48.97%), Postives = 109/145 (75.17%), Query Frame = 1 
Query:   34 FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            FE  D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT +K ED+ PST N D+P +KR ++QL+ I  DGY+SL+ + G+V+ED++LP G+L  +++ +++ G+ +++TV+SA +EE  V+ +
Sbjct:    7 FETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQ-DGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Mouse
Match: Eif5a (eukaryotic translation initiation factor 5A [Source:MGI Symbol;Acc:MGI:106248])

HSP 1 Score: 157.918 bits (398), Expect = 9.942e-48
Identity = 71/145 (48.97%), Postives = 109/145 (75.17%), Query Frame = 1 
Query:   34 FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            FE  D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT +K ED+ PST N D+P +KR ++QL+ I  DGY+SL+ + G+V+ED++LP G+L  +++ +++ G+ +++TV+SA +EE  V+ +
Sbjct:    7 FETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQ-DGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Mouse
Match: Eif5a (eukaryotic translation initiation factor 5A [Source:MGI Symbol;Acc:MGI:106248])

HSP 1 Score: 157.918 bits (398), Expect = 9.942e-48
Identity = 71/145 (48.97%), Postives = 109/145 (75.17%), Query Frame = 1 
Query:   34 FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            FE  D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT +K ED+ PST N D+P +KR ++QL+ I  DGY+SL+ + G+V+ED++LP G+L  +++ +++ G+ +++TV+SA +EE  V+ +
Sbjct:    7 FETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQ-DGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIK 150
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. UniProt/SwissProt
Match: sp|P23301|IF5A1_YEAST (Eukaryotic translation initiation factor 5A-1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HYP2 PE=1 SV=3)

HSP 1 Score: 176.792 bits (447), Expect = 3.205e-54
Identity = 86/152 (56.58%), Postives = 113/152 (74.34%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            MS+ E TFE  D+G+S T P Q SA RK  +++IK RPCKI+DMST KTGKHG AKVH+ A+DIFT +K ED+SPST N ++P+VKR EYQL+DI  DG++SLM+  GD K+DV+ P GEL   L+T F+ G  +MVT++SA  EE  +S +
Sbjct:    1 MSDEEHTFETADAGSSATYPMQCSALRKNGFVVIKSRPCKIVDMSTSKTGKHGHAKVHLVAIDIFTGKKLEDLSPSTHNMEVPVVKRNEYQLLDID-DGFLSLMNMDGDTKDDVKAPEGELGDSLQTAFDEGKDLMVTIISAMGEEAAISFK 151
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. UniProt/SwissProt
Match: sp|P19211|IF5A2_YEAST (Eukaryotic translation initiation factor 5A-2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ANB1 PE=1 SV=3)

HSP 1 Score: 174.096 bits (440), Expect = 3.019e-53
Identity = 85/152 (55.92%), Postives = 111/152 (73.03%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            MS+ E TFEN D+GAS T P Q SA RK  +++IK RPCKI+DMST KTGKHG AKVH+  +DIFT +K ED+SPST N ++P VKR EYQL+DI  DGY+SLM+  G+ K+DV+ P GEL   ++  F+ G  +MVT++SA  EE  +S +
Sbjct:    1 MSDEEHTFENADAGASATYPMQCSALRKNGFVVIKGRPCKIVDMSTSKTGKHGHAKVHLVTLDIFTGKKLEDLSPSTHNLEVPFVKRSEYQLLDID-DGYLSLMTMDGETKDDVKAPEGELGDSMQAAFDEGKDLMVTIISAMGEEAAISFK 151
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. UniProt/SwissProt
Match: sp|O94083|IF5A_CANAL (Eukaryotic translation initiation factor 5A OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ANB1 PE=3 SV=2)

HSP 1 Score: 173.711 bits (439), Expect = 4.912e-53
Identity = 83/152 (54.61%), Postives = 114/152 (75.00%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            M+E + TFE  D+GA+LT P Q SA RK  +++IK+RPCKI+DMST KTGKHG AKVH+ A+DIFT +K ED+SPST N ++P V R E+QL+DI  DGY+SLM+  GD K+DV++P GEL   L++ F+ G  ++VT++SA  EE  +S +
Sbjct:    1 MAEEDHTFETADAGAALTFPMQCSALRKNGHVVIKNRPCKIVDMSTSKTGKHGHAKVHLVAIDIFTGKKLEDLSPSTHNMEVPNVSRQEFQLLDID-DGYLSLMTADGDTKDDVKVPEGELGDKLQSEFDEGKDLIVTIISAMGEEAAISYK 151
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. UniProt/SwissProt
Match: sp|P62925|IF5A_SPOFR (Eukaryotic translation initiation factor 5A OS=Spodoptera frugiperda OX=7108 GN=eIF-5A PE=1 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 7.164e-53
Identity = 80/156 (51.28%), Postives = 115/156 (73.72%), Query Frame = 1 
Query:   13 MSEFEDT-FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVT 477
            M++ EDT FE  DSGAS T P Q SA RK  ++M+K RPCKI++MST KTGKHG AKVH+  +DIF  +K ED+ PST N D+P VKR +YQL DIS DGY++LM++ GD++ED+++P G+L   L++ F++G  ++ TV+ +C EE +++ +  T
Sbjct:    1 MADIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRSDFDSGKELLCTVLKSCGEECVIAVKANT 156
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. UniProt/SwissProt
Match: sp|P62924|IF5A_SPOEX (Eukaryotic translation initiation factor 5A OS=Spodoptera exigua OX=7107 GN=eIF-5A PE=2 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 7.164e-53
Identity = 80/156 (51.28%), Postives = 115/156 (73.72%), Query Frame = 1 
Query:   13 MSEFEDT-FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVT 477
            M++ EDT FE  DSGAS T P Q SA RK  ++M+K RPCKI++MST KTGKHG AKVH+  +DIF  +K ED+ PST N D+P VKR +YQL DIS DGY++LM++ GD++ED+++P G+L   L++ F++G  ++ TV+ +C EE +++ +  T
Sbjct:    1 MADIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRSDFDSGKELLCTVLKSCGEECVIAVKANT 156
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. TrEMBL
Match: D5JG61 (Eukaryotic translation initiation factor 5A (Fragment) OS=Dugesia japonica OX=6161 GN=eIF5A PE=2 SV=1)

HSP 1 Score: 219.55 bits (558), Expect = 1.146e-68
Identity = 100/149 (67.11%), Postives = 124/149 (83.22%), Query Frame = 1 
Query:   31 TFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVT 477
            TFEN DSGAS TVP QMSAFRK Q+IMIKDRPCKI++MST KTGKHGSAKVH+ A+DIF+ +K ED+ PSTAN+D PIV RIEYQL+DIS D + SL+++KGD KED++LP  EL  +++T+F NGD  +VTVMSAC EE+IV C+ +T
Sbjct:    2 TFENTDSGASSTVPQQMSAFRKNQHIMIKDRPCKIVEMSTSKTGKHGSAKVHIVAIDIFSGKKYEDICPSTANKDKPIVNRIEYQLIDISNDNFTSLLNDKGDTKEDIKLPENELADEIRTKFSNGDSCVVTVMSACGEEQIVGCKVLT 150
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. TrEMBL
Match: A0A1D2N039 (Eukaryotic translation initiation factor 5A OS=Orchesella cincta OX=48709 GN=Ocin01_08026 PE=3 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 2.895e-54
Identity = 83/156 (53.21%), Postives = 118/156 (75.64%), Query Frame = 1 
Query:   13 MSEFEDT-FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVT 477
            M + +DT FE  DSGAS T P Q SA RK  ++M+K RPCKI++MST KTGKHG AKVH+  +DIFT +KCED+ PST N D+P VKR +YQL+DIS DG++SLM + G+++ED+++P G+L + LKT ++NG  ++ TV+ +C EE +++ +N T
Sbjct:    1 MGDIDDTQFEAADSGASATYPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHMVGLDIFTGKKCEDICPSTHNMDVPFVKREDYQLMDISDDGFLSLMGDSGELREDLKIPDGDLGSQLKTEYDNGREILCTVLKSCGEECVIAIKNNT 156
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. TrEMBL
Match: A0A1G4JA14 (Eukaryotic translation initiation factor 5A OS=Lachancea sp. CBS 6924 OX=433476 GN=LAFA_0E02058G PE=3 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 5.506e-54
Identity = 88/152 (57.89%), Postives = 113/152 (74.34%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            M+E E TFE  D+G+SLT P Q SA RK  +++IK RPCKI+DMST KTGKHG AKVH+ A+DIFT +K ED+SPST N D+P VKR EYQL+DI  DGY+SLM+  GD K+DV+ P GE+   ++  FE G  +MVT++SA  EE  +SC+
Sbjct:    1 MAEEEHTFETADAGSSLTYPMQCSALRKNGFVVIKSRPCKIVDMSTSKTGKHGHAKVHLVAIDIFTGKKLEDLSPSTHNMDVPNVKRTEYQLLDID-DGYLSLMTMDGDTKDDVRAPDGEIGEQIQAGFEEGKDMMVTIISAMGEEAAISCK 151
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. TrEMBL
Match: C5DDS7 (Eukaryotic translation initiation factor 5A OS=Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284) OX=559295 GN=KLTH0C03476g PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 1.816e-53
Identity = 87/152 (57.24%), Postives = 115/152 (75.66%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            M+E E TFE  D+G+SLT P Q SA RK  +++IK RPCKI+DMST KTGKHG AKVH+ A+DIFT +K ED+SPST N D+P VKR E+QL+DI  DGY+SLM+ +GD K+DV+ P GEL   +++ F+ G  +MVT++SA  EE  +SC+
Sbjct:    1 MAEEEHTFETADAGSSLTFPMQCSALRKNGFVVIKGRPCKIVDMSTSKTGKHGHAKVHLVAIDIFTGKKLEDLSPSTHNMDVPNVKRTEFQLLDID-DGYLSLMTMEGDTKDDVRAPEGELGDQIQSGFDEGKDLMVTIISAMGEEAAISCK 151
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. TrEMBL
Match: A0A1G4K0W6 (Eukaryotic translation initiation factor 5A OS=Lachancea dasiensis CBS 10888 OX=1266660 GN=LADA_0H04896G PE=3 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 2.894e-53
Identity = 86/152 (56.58%), Postives = 115/152 (75.66%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            M+E E TFE VD+G+SLT P Q SA RK  +++IK RPCKI+DMST KTGKHG AKVH+ A+DIFT +K ED+SPST N D+P VKR EYQL+DI  DG++SLM+  GD K+DV+ P G++   +++ F+ G  +MVT++SA  EE  +SC+
Sbjct:    1 MAEEEHTFETVDAGSSLTYPMQCSALRKNGFVVIKSRPCKIVDMSTSKTGKHGHAKVHLVAIDIFTGKKLEDLSPSTHNMDVPNVKRTEYQLLDID-DGFLSLMTADGDTKDDVRTPDGDIGEQIQSGFDEGKDLMVTIISAMGEEAAISCK 151
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Cavefish
Match: eif5a2 (eukaryotic translation initiation factor 5A-1-like [Source:NCBI gene;Acc:103022137])

HSP 1 Score: 162.54 bits (410), Expect = 1.283e-49
Identity = 74/156 (47.44%), Postives = 118/156 (75.64%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVTN 480
            MS+ +  F + D+GAS+T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT +K ED+ PST N D+P +KR++YQLV + T+GY+SLM + G+++ED++LP  EL  +++++F+ G+ ++++V+SA  EE  V+ + +T+
Sbjct:    1 MSDPDVDFTSGDAGASMTYPMQCSALRKNGHVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKNEDICPSTHNMDVPNIKRLDYQLVGV-TEGYLSLMKDNGEIREDLKLPDSELGKEIESKFDAGEEILISVLSAMGEECAVAIKAMTS 155
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Cavefish
Match: EIF5AL1 (eukaryotic translation initiation factor 5A [Source:NCBI gene;Acc:103038290])

HSP 1 Score: 160.229 bits (404), Expect = 1.975e-48
Identity = 73/159 (45.91%), Postives = 115/159 (72.33%), Query Frame = 1 
Query:    1 FF*KMSEFEDT---FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            F  +++E  D    F   D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIF+ +K ED+ PST N D+P +KR++YQL+ I  DGY+SL+ + G+V+ED+++P G+L  +++ +F+ G+ +++TV+SA +EE  V+ +
Sbjct:   22 FLSQLTEMADADLDFTTGDAGASATYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHMVGIDIFSGKKYEDICPSTHNMDVPNIKRMDYQLIGIQ-DGYLSLLQDSGEVREDLKMPEGDLGKEIEGKFDAGEEILITVLSAMNEEAAVAIK 179
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Cavefish
Match: EIF5AL1 (eukaryotic translation initiation factor 5A [Source:NCBI gene;Acc:103038290])

HSP 1 Score: 158.688 bits (400), Expect = 3.864e-48
Identity = 71/152 (46.71%), Postives = 113/152 (74.34%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            M++ +  F   D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIF+ +K ED+ PST N D+P +KR++YQL+ I  DGY+SL+ + G+V+ED+++P G+L  +++ +F+ G+ +++TV+SA +EE  V+ +
Sbjct:    1 MADADLDFTTGDAGASATYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHMVGIDIFSGKKYEDICPSTHNMDVPNIKRMDYQLIGIQ-DGYLSLLQDSGEVREDLKMPEGDLGKEIEGKFDAGEEILITVLSAMNEEAAVAIK 151
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Cavefish
Match: EIF5AL1 (eukaryotic translation initiation factor 5A [Source:NCBI gene;Acc:103038290])

HSP 1 Score: 158.688 bits (400), Expect = 3.864e-48
Identity = 71/152 (46.71%), Postives = 113/152 (74.34%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            M++ +  F   D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIF+ +K ED+ PST N D+P +KR++YQL+ I  DGY+SL+ + G+V+ED+++P G+L  +++ +F+ G+ +++TV+SA +EE  V+ +
Sbjct:    1 MADADLDFTTGDAGASATYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHMVGIDIFSGKKYEDICPSTHNMDVPNIKRMDYQLIGIQ-DGYLSLLQDSGEVREDLKMPEGDLGKEIEGKFDAGEEILITVLSAMNEEAAVAIK 151
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Cavefish
Match: eif5a (eukaryotic translation initiation factor 5A-1 [Source:NCBI gene;Acc:103024997])

HSP 1 Score: 158.303 bits (399), Expect = 5.172e-48
Identity = 74/155 (47.74%), Postives = 115/155 (74.19%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVT 477
            M++ +  F + D+GAS+T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIFT++K ED+ PST N D+P +KR ++QLVDI+ +G++SLM + GDV+ED+++P G+L  +++++   G+ +M+TV+SA  EE  V  +  T
Sbjct:    1 MADPDLDFTSGDAGASVTYPQQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTNKKYEDICPSTHNMDVPNIKRNDFQLVDIN-EGFLSLMMDNGDVREDLRVPDGDLGKEIESKHGAGEDMMITVLSAMGEEAAVGIKAFT 154
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Yeast
Match: HYP2 (Translation elongation factor eIF-5A; required for translation of proteins containing polyproline stretches, including Bni1p, and this leads to a requirement for mating projection formation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication; human EIF5A complements the inviability of the yeast hyp2 anb1 double null mutant [Source:SGD;Acc:S000000760])

HSP 1 Score: 176.792 bits (447), Expect = 4.615e-56
Identity = 86/152 (56.58%), Postives = 113/152 (74.34%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            MS+ E TFE  D+G+S T P Q SA RK  +++IK RPCKI+DMST KTGKHG AKVH+ A+DIFT +K ED+SPST N ++P+VKR EYQL+DI  DG++SLM+  GD K+DV+ P GEL   L+T F+ G  +MVT++SA  EE  +S +
Sbjct:    1 MSDEEHTFETADAGSSATYPMQCSALRKNGFVVIKSRPCKIVDMSTSKTGKHGHAKVHLVAIDIFTGKKLEDLSPSTHNMEVPVVKRNEYQLLDID-DGFLSLMNMDGDTKDDVKAPEGELGDSLQTAFDEGKDLMVTIISAMGEEAAISFK 151
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Yeast
Match: ANB1 (Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication; human EIF5A complements the inviability of the yeast hyp2 anb1 double null mutant [Source:SGD;Acc:S000003808])

HSP 1 Score: 174.096 bits (440), Expect = 4.348e-55
Identity = 85/152 (55.92%), Postives = 111/152 (73.03%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            MS+ E TFEN D+GAS T P Q SA RK  +++IK RPCKI+DMST KTGKHG AKVH+  +DIFT +K ED+SPST N ++P VKR EYQL+DI  DGY+SLM+  G+ K+DV+ P GEL   ++  F+ G  +MVT++SA  EE  +S +
Sbjct:    1 MSDEEHTFENADAGASATYPMQCSALRKNGFVVIKGRPCKIVDMSTSKTGKHGHAKVHLVTLDIFTGKKLEDLSPSTHNLEVPFVKRSEYQLLDID-DGYLSLMTMDGETKDDVKAPEGELGDSMQAAFDEGKDLMVTIISAMGEEAAISFK 151
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Yeast
Match: YEL034C-A_mRNA (Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps HYP2/YEL034W, a verified gene that encodes eiF-5A [Source:SGD;Acc:S000028743])

HSP 1 Score: 67.3958 bits (163), Expect = 6.369e-14
Identity = 45/137 (32.85%), Postives = 66/137 (48.18%), Query Frame = -3 
Query:   55 NNLFFTTCRHYGNHYHVSVFESCFQISL*FTTW*LYIFFNVSLFRH*TYIAISRYINKLIFNSLHNWHVSISS*WRHIFAFSISKNINSCNMDFS*TMFTCLTCTHIDNFTRSIF--NHYILTFAESTHLIRNCKRS 459
            +  FFT     GNH  ++  +S  Q    FT W L I  +V++  H     +   + +L+F SL NW+  + S WR IF    S++IN   MDFS T+ T L   H+DN T S    N  +L+   + H +     S
Sbjct:   33 DGGFFTHSGDDGNHQVLTFIKSSLQTVTQFTFWSLDIILSVTVHVHQRQETVIN-VQQLVFVSLDNWNFHVVSRWRQIFQLLTSEDINGNQMDFSVTVLTSLRSGHVDNLTWSTLDDNETVLSQGRTLHWVGGGGTS 168
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Nematostella
Match: EDO30482 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T0N1])

HSP 1 Score: 150.214 bits (378), Expect = 2.938e-45
Identity = 70/151 (46.36%), Postives = 104/151 (68.87%), Query Frame = 1 
Query:   19 EFEDT-FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            E  DT F + +SGAS T P Q S+ RK  +++IK RPCKI++MST KTGKHG AKVH+ A+DIFT++K ED+ PST N ++P V R ++Q+ +I  DGY+ LM + GD + D++L   ++  +++ +FE  +  MVTV+ A  EE +V  +
Sbjct:    4 ELADTEFHSGESGASDTYPAQCSSLRKNGHVVIKGRPCKIVEMSTSKTGKHGHAKVHLVALDIFTNKKYEDICPSTHNMNVPHVSRADFQVTNIEEDGYLELMDDNGDTRADIKLQDNDIAKEIRAKFEASENFMVTVLKAMGEETVVGVK 154
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Medaka
Match: ENSORLT00000044399.1 (eukaryotic translation initiation factor 5A-1 [Source:NCBI gene;Acc:101166426])

HSP 1 Score: 163.31 bits (412), Expect = 2.380e-49
Identity = 75/152 (49.34%), Postives = 111/152 (73.03%), Query Frame = 1 
Query:   25 EDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVTN 480
            +D F   D+GAS T P Q SA RK  ++M+K RPCKI++M+T KTGKHG AKVH+T +DIFT +K ED+ PST N D+P V R +YQ +D+S DG++SLM + G+ +ED+++P G+L  ++  +  NG++ MV+V+ A  EE+IV  +N+T+
Sbjct:   47 DDDFSTADAGASTTFPMQCSALRKNGFVMLKGRPCKIVEMTTSKTGKHGHAKVHLTGLDIFTQKKYEDICPSTHNMDVPNVIRKDYQAIDVS-DGFLSLMDDNGETREDLKVPEGDLGKEIIKKISNGELFMVSVLKAVDEEQIVGTKNMTS 197
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Medaka
Match: EIF5AL1 (eukaryotic translation initiation factor 5A [Source:NCBI gene;Acc:101159469])

HSP 1 Score: 160.999 bits (406), Expect = 4.877e-49
Identity = 71/154 (46.10%), Postives = 114/154 (74.03%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNV 474
            M++ E  F+  D+GAS T P Q SA RK  ++++K RPCKI++MST KTGKHG AKVH+  +DIF+ +K ED+ PST N D+P +KR++YQL+ I  DGY+SL+ + G+V+ED+++P G+L  ++++++E G+ ++VTV++A  EE  +  + +
Sbjct:    1 MADAELDFQTGDAGASATYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFSGKKYEDICPSTHNMDVPNIKRMDYQLIGIQ-DGYLSLLQDSGEVREDLKIPEGDLGKEIESKYEAGEEILVTVLNAMDEEAAIGIKAL 153
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Medaka
Match: ENSORLT00000028460.1 (eukaryotic translation initiation factor 5A-1 [Source:NCBI gene;Acc:101154812])

HSP 1 Score: 156.762 bits (395), Expect = 1.207e-46
Identity = 75/145 (51.72%), Postives = 109/145 (75.17%), Query Frame = 1 
Query:   34 FENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCR 468
            F   +SGAS T P Q SA RK  Y+++K RPCKI++MST KTGKHG AKV++  +DIFT++K ED+ PST N D+P +KRI+YQLV+I+ + Y+SLMS+ GD++ED+++P  E+  +++ +FE G+  MVTV+SA  EE  V+ +
Sbjct:   68 FTTGESGASATYPMQCSALRKNGYVVLKGRPCKIVEMSTSKTGKHGHAKVNLVGIDIFTNKKYEDMCPSTHNMDVPSIKRIDYQLVNIN-ENYMSLMSDNGDIREDLRVPENEVGKEIEAKFEAGEEFMVTVISAMGEECAVATK 211
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Planmine SMEST
Match: SMESG000081077.1 (SMESG000081077.1)

HSP 1 Score: 276.559 bits (706), Expect = 7.138e-95
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 1 
Query:   79 MSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVTN 480
            MSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVTN
Sbjct:    1 MSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVTN 134
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Planmine SMEST
Match: SMESG000023236.1 (SMESG000023236.1)

HSP 1 Score: 271.552 bits (693), Expect = 6.432e-92
Identity = 126/155 (81.29%), Postives = 142/155 (91.61%), Query Frame = 1 
Query:   16 SEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVTN 480
            S++E+TF+NVDSGASLTVP QMSAFRKGQYIMIKDRPCKI DMSTCKTGKHGSAKV VTA DIFTD++ EDVSPSTANR+ PIVKRIE+QL+DIS D Y SLMSEKGDVKEDVQLPGGELEAD++ RFE GD VMVTVMSAC+EEK+V+C+ +TN
Sbjct:   53 SDYEETFDNVDSGASLTVPKQMSAFRKGQYIMIKDRPCKITDMSTCKTGKHGSAKVQVTATDIFTDKRLEDVSPSTANRETPIVKRIEFQLIDISGDNYTSLMSEKGDVKEDVQLPGGELEADIRARFEKGDHVMVTVMSACNEEKVVACKTITN 207
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Planmine SMEST
Match: SMESG000023224.1 (SMESG000023224.1)

HSP 1 Score: 264.233 bits (674), Expect = 1.179e-89
Identity = 122/156 (78.21%), Postives = 141/156 (90.38%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVTN 480
            MS++E+TF+NVDSGASLTVP QMSAFRKGQYIMIKD+PCKI DMSTCKTGKHGSAKV V A DIFT+++ EDVSPSTANR+ PIVKRIE+QL+DIS D Y SLMSE+GD+KEDVQLPGGELEA+++ RFE GD V+VTVMSAC+EEKIVS + VTN
Sbjct:    1 MSDYEETFDNVDSGASLTVPKQMSAFRKGQYIMIKDKPCKITDMSTCKTGKHGSAKVQVVATDIFTEKRLEDVSPSTANRETPIVKRIEFQLIDISGDNYTSLMSERGDIKEDVQLPGGELEAEIRARFEKGDQVVVTVMSACNEEKIVSVKTVTN 156
[Close]
BLAST of Eukaryotic translation initiation factor 5A vs. Planmine SMEST
Match: SMESG000016245.1 (SMESG000016245.1)

HSP 1 Score: 228.024 bits (580), Expect = 2.155e-75
Identity = 104/156 (66.67%), Postives = 130/156 (83.33%), Query Frame = 1 
Query:   13 MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTGKHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGYVSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVSCRNVTN 480
            MS+ EDTFEN DSGAS TVP QMSAFRK Q+IMIKDRPCKI++MST KTGKHGSAKVHV  +DIF+ +K ED+ PSTAN+D P+V R+EY L+DISTD YVSLM++KG+ KEDV+LP  EL  +++ +FE+GD  +VT+MSAC EE+IV C+ +TN
Sbjct:    1 MSDIEDTFENADSGASQTVPQQMSAFRKNQHIMIKDRPCKIVEMSTSKTGKHGSAKVHVVGIDIFSGKKYEDICPSTANKDKPLVNRVEYTLIDISTDNYVSLMNDKGETKEDVKLPENELADEIRAKFESGDNCLVTIMSACGEEQIVGCKVLTN 156
The following BLAST results are available for this feature:
BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
EIF5A1.448e-4748.97eukaryotic translation initiation factor 5A [Sourc... [more]
EIF5A1.448e-4748.97eukaryotic translation initiation factor 5A [Sourc... [more]
EIF5A1.448e-4748.97eukaryotic translation initiation factor 5A [Sourc... [more]
EIF5A1.448e-4748.97eukaryotic translation initiation factor 5A [Sourc... [more]
EIF5A1.448e-4748.97eukaryotic translation initiation factor 5A [Sourc... [more]
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BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
iff-18.579e-4449.66Eukaryotic translation initiation factor 5A-1 [So... [more]
iff-18.579e-4449.66Eukaryotic translation initiation factor 5A-1 [So... [more]
iff-11.847e-4349.66Eukaryotic translation initiation factor 5A-1 [So... [more]
iff-24.353e-4252.45Eukaryotic translation initiation factor 5A-2 [So... [more]
iff-24.353e-4252.45Eukaryotic translation initiation factor 5A-2 [So... [more]
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BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
eEF53.832e-5350.32gene:FBgn0285952 transcript:FBtr0072172[more]
eEF53.832e-5350.32gene:FBgn0285952 transcript:FBtr0072173[more]
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BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
eif5a8.011e-5049.68eukaryotic translation initiation factor 5A [Sourc... [more]
eif5a8.011e-5049.68eukaryotic translation initiation factor 5A [Sourc... [more]
eif5a8.011e-5049.68eukaryotic translation initiation factor 5A [Sourc... [more]
eif5a8.011e-5049.68eukaryotic translation initiation factor 5A [Sourc... [more]
eif5a8.011e-5049.68eukaryotic translation initiation factor 5A [Sourc... [more]
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BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ddo2.501e-4648.65D-aspartate oxidase [Source:Xenbase;Acc:XB-GENE-62... [more]
ddo3.885e-4248.87D-aspartate oxidase [Source:Xenbase;Acc:XB-GENE-62... [more]
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BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Eif5a9.942e-4848.97eukaryotic translation initiation factor 5A [Sourc... [more]
Eif5a9.942e-4848.97eukaryotic translation initiation factor 5A [Sourc... [more]
Eif5a9.942e-4848.97eukaryotic translation initiation factor 5A [Sourc... [more]
Eif5a9.942e-4848.97eukaryotic translation initiation factor 5A [Sourc... [more]
Eif5a9.942e-4848.97eukaryotic translation initiation factor 5A [Sourc... [more]
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BLAST of Eukaryotic translation initiation factor 5A vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P23301|IF5A1_YEAST3.205e-5456.58Eukaryotic translation initiation factor 5A-1 OS=S... [more]
sp|P19211|IF5A2_YEAST3.019e-5355.92Eukaryotic translation initiation factor 5A-2 OS=S... [more]
sp|O94083|IF5A_CANAL4.912e-5354.61Eukaryotic translation initiation factor 5A OS=Can... [more]
sp|P62925|IF5A_SPOFR7.164e-5351.28Eukaryotic translation initiation factor 5A OS=Spo... [more]
sp|P62924|IF5A_SPOEX7.164e-5351.28Eukaryotic translation initiation factor 5A OS=Spo... [more]
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BLAST of Eukaryotic translation initiation factor 5A vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
D5JG611.146e-6867.11Eukaryotic translation initiation factor 5A (Fragm... [more]
A0A1D2N0392.895e-5453.21Eukaryotic translation initiation factor 5A OS=Orc... [more]
A0A1G4JA145.506e-5457.89Eukaryotic translation initiation factor 5A OS=Lac... [more]
C5DDS71.816e-5357.24Eukaryotic translation initiation factor 5A OS=Lac... [more]
A0A1G4K0W62.894e-5356.58Eukaryotic translation initiation factor 5A OS=Lac... [more]
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BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
eif5a21.283e-4947.44eukaryotic translation initiation factor 5A-1-like... [more]
EIF5AL11.975e-4845.91eukaryotic translation initiation factor 5A [Sourc... [more]
EIF5AL13.864e-4846.71eukaryotic translation initiation factor 5A [Sourc... [more]
EIF5AL13.864e-4846.71eukaryotic translation initiation factor 5A [Sourc... [more]
eif5a5.172e-4847.74eukaryotic translation initiation factor 5A-1 [Sou... [more]
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BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
HYP24.615e-5656.58Translation elongation factor eIF-5A; required for... [more]
ANB14.348e-5555.92Translation elongation factor eIF-5A; previously t... [more]
YEL034C-A_mRNA6.369e-1432.85Dubious open reading frame; unlikely to encode a f... [more]
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BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO304822.938e-4546.36Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Eukaryotic translation initiation factor 5A vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSORLT00000044399.12.380e-4949.34eukaryotic translation initiation factor 5A-1 [Sou... [more]
EIF5AL14.877e-4946.10eukaryotic translation initiation factor 5A [Sourc... [more]
ENSORLT00000028460.11.207e-4651.72eukaryotic translation initiation factor 5A-1 [Sou... [more]
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BLAST of Eukaryotic translation initiation factor 5A vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000081077.17.138e-95100.00SMESG000081077.1[more]
SMESG000023236.16.432e-9281.29SMESG000023236.1[more]
SMESG000023224.11.179e-8978.21SMESG000023224.1[more]
SMESG000016245.12.155e-7566.67SMESG000016245.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034271 ID=SMED30034271|Name=Eukaryotic translation initiation factor 5A|organism=Schmidtea mediterranea sexual|type=transcript|length=887bp
TTTTTTTGAAAAATGTCAGAATTTGAAGATACTTTTGAAAATGTTGACTC
CGGAGCTTCTCTTACAGTTCCTAATCAAATGAGTGCTTTCCGCAAAGGTC
AATATATAATGATTAAAGATAGACCTTGTAAAATTATCGATATGAGTACA
TGTAAGACAGGTAAACATGGTTCAGCTAAAGTCCATGTTACAGCTGTTGA
TATTTTTACTGATAGAAAATGCGAAGATGTGTCTCCATCAACTGCTAATA
GAGACATGCCAATTGTGAAGAGAATTGAATATCAACTTGTTGATATATCG
ACTGATGGCTATGTAAGTCTAATGTCTGAAAAGGGAGACGTTAAAGAAGA
TGTACAACTACCAGGTGGTGAATTAGAGGCTGATTTGAAAACAAGATTCG
AAAACGGAGACATGGTAATGGTTACCGTAATGTCTGCATGTAGTGAAGAA
AAGATTGTTTCGTGCCGAAATGTCACTAATTAAACTGCTTTCTATGTCAG
AAGATGAATTGAATAAAATAATTTTGTATGAGACAAAGTTTTGATACAAT
GTTGAATATTTAAAAACTTATTGGTTAAATATAATTAGATATTTATTTAA
AAAAATAAATGCATGTTACGATTTCAGTATTTAATAAAAAGTTTATGGTT
AATTATTATGCAATTTTGTATTTAATGGTATGCTATTGGCTGTAATGTCG
TGGCTAATGCTTCGAGAATATAAACTATTTTTATTTAGAATGTTGATTAC
GAAAACTTAGTTAAAAACTAATTGTGTATATTATTTTAAAAGTTAGCTCG
GCTTTATTCTATGTGTTGTGAGTCACAAGCTGAATTATGTCAAGCTACCA
TTGTGTCATTGTAGATTAGATTTTTTAAAATAATGTC
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protein sequence of SMED30034271-orf-1

>SMED30034271-orf-1 ID=SMED30034271-orf-1|Name=SMED30034271-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=157bp
MSEFEDTFENVDSGASLTVPNQMSAFRKGQYIMIKDRPCKIIDMSTCKTG
KHGSAKVHVTAVDIFTDRKCEDVSPSTANRDMPIVKRIEYQLVDISTDGY
VSLMSEKGDVKEDVQLPGGELEADLKTRFENGDMVMVTVMSACSEEKIVS
CRNVTN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000429neoblast
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR020189Transl_elong_IF5A_C
IPR012340NA-bd_OB-fold
IPR014722Rib_L2_dom2
IPR001884IF5A-like
IPR013185Transl_elong_KOW-like
IPR019769Trans_elong_IF5A_hypusine_site
IPR008991Translation_prot_SH3-like_sf
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
GO:0003746translation elongation factor activity
GO:0043022ribosome binding
GO:0003743translation initiation factor activity
Vocabulary: biological process
TermDefinition
GO:0006452translational frameshifting
GO:0045901positive regulation of translational elongation
GO:0045905positive regulation of translational termination
GO:0006412translation
GO:0006413translational initiation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020189Translation elongation factor, IF5A C-terminalSMARTSM01376eIF_5a_2coord: 84..152
e-value: 7.8E-29
score: 111.8
IPR020189Translation elongation factor, IF5A C-terminalPFAMPF01287eIF-5acoord: 84..152
e-value: 1.5E-26
score: 92.3
IPR001884Translation elongation factor IF5A-likePIRSFPIRSF003025Transl_init_eIF5Acoord: 1..156
e-value: 1.5E-58
score: 194.4
IPR001884Translation elongation factor IF5A-likeTIGRFAMTIGR00037TIGR00037coord: 18..150
e-value: 6.8E-42
score: 140.3
IPR001884Translation elongation factor IF5A-likePANTHERPTHR11673TRANSLATION INITIATION FACTOR 5A-RELATEDcoord: 1..154
NoneNo IPR availableGENE3DG3DSA:2.40.50.140coord: 85..156
e-value: 7.6E-27
score: 95.3
NoneNo IPR availablePANTHERPTHR11673:SF6EUKARYOTIC TRANSLATION INITIATION FACTOR 5Acoord: 1..154
NoneNo IPR availableCDDcd04468S1_eIF5Acoord: 85..153
e-value: 6.02646E-28
score: 96.8376
IPR013185Translation elongation factor, KOW-likePFAMPF08207EFP_Ncoord: 23..71
e-value: 9.5E-5
score: 22.3
IPR014722Ribosomal protein L2, domain 2GENE3DG3DSA:2.30.30.30coord: 1..84
e-value: 1.2E-34
score: 119.9
IPR019769Translation elongation factor, IF5A, hypusine sitePROSITEPS00302IF5A_HYPUSINEcoord: 49..56
IPR008991Translation protein SH3-like domain superfamilySUPERFAMILYSSF50104Translation proteins SH3-like domaincoord: 14..92
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILYSSF50249Nucleic acid-binding proteinscoord: 85..154