AAA family ATPase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30034256 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of AAA family ATPase vs. Ensembl Human
Match: SPATA5 (spermatogenesis associated 5 [Source:HGNC Symbol;Acc:HGNC:18119]) HSP 1 Score: 165.236 bits (417), Expect = 7.132e-41 Identity = 93/241 (38.59%), Postives = 140/241 (58.09%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR-DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
PS + D+ + W DIGG ES+K+ L+ +E PLK+ + F+ KGVL +GPPGCSKTM +ALA E+GLN ++IK +M YVGESE +R+ F +A A+ PSI+F DE+D + R L V+ R++++LL ++GI + +V +AATN D++D A+MR GR+ I + LPD +R I + F +++V + + + SGAEIV R+ L
Sbjct: 611 PSAMREIAIDVPNVSWSDIGGLESIKLKLEQAVEWPLKHPESFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNKYVGESERAVRETFRKARAVAPSIIFFDELDALAVERGSSLGAGNVADRVLAQLLTEMDGIEQLKDVTILAATNRPDRIDKALMRPGRIDRIIYVPLPDAATRREIFKLQFHS----MPVSNEV--DLDELILQTDAYSGAEIVAVCREAAL 845 HSP 2 Score: 159.073 bits (401), Expect = 4.907e-39 Identity = 108/368 (29.35%), Postives = 189/368 (51.36%), Query Frame = 3
Query: 2253 GFVNKKVCMSLP--KDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSAS----------------VKYS---RTDMRQLIEFSREIENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSN-SKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHNNNENVSDSLPTIKYQYFREAIS 3266
G +K++ + +P +D + L+ ++ ++ ++ + E + +L + HG+ D++ A VK + + ++ ++ +I ++ +E+ + W+DIGG E++K L + +E P+KH G++ PKG+L +GPPGC KTM ALA E L + ++M VGESE A R +A +PS+IF +++D L +R S+ NV +R+L++L+T + GIE K +V I+A TN +D+AL+RPGR+ I+ + LPD R I K+ F + E ++ + SGAEIV + RE A E++ +L I ++F +A+S
Sbjct: 513 GRFDKEIEIGVPNAQDRLDILQKLLRRVPHLLTEAE-LLQLANSAHGYVGADLKVLCNEAGLCALRRILKKQPNLPDVKVAGLVKITLKDFLQAMNDIRPSAMREIAIDVPNVSWSDIGGLESIKLKLEQAVEWPLKHPESFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNKYVGESERAVRETFRKARAVAPSIIFFDELDALAVERGSSLGAGNVADRVLAQLLTEMDGIEQLK--DVTILAATNRPDRIDKALMRPGRIDRIIYVPLPDAATRREIFKLQFHSMPVSNE----VDLDELILQTDAYSGAEIVAVCRE----AALLALEEDIQANL--IMKRHFTQALS 867 HSP 3 Score: 127.872 bits (320), Expect = 3.530e-29 Identity = 79/235 (33.62%), Postives = 134/235 (57.02%), Query Frame = 3
Query: 2478 EIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIK-------HGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQN-VMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDL-NFQTEHEIISNISTIAESLTGLSGAEI 3155
EI+ SKE D+Q + + + IGG + + E IE+P+K +G+ P+G+L +GPPG KTM A+A E + + +I+ GE+EA R I ++A PS+IFI+++D L PKR +++ V +R+++ L+T + GI ++ ++ V+++ TN H +D AL RPGR + IG+P+ +DR IL+ + + TE E++ +A S G GA++
Sbjct: 336 EIDKNSKEQDNQFK-VTYDMIGGLSSQLKAIREIIELPLKQPELFKSYGIPAPRGVLLYGPPGTGKTMIARAVANEVGAYVSVINGPEIISKFYGETEAKLRQIFAEATLRHPSIIFIDELDALCPKR-EGAQNEVEKRVVASLLTLMDGIGSEVSEGQVLVLGATNRPHALDAALRRPGRFDKEIEIGVPNAQDRLDILQKLLRRVPHLLTEAELLQ----LANSAHGYVGADL 564 HSP 4 Score: 117.087 bits (292), Expect = 7.307e-26 Identity = 72/180 (40.00%), Postives = 99/180 (55.00%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGIN---EKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
IGG S ++ IE PLK + F +GVL +GPPG KTM RA+A E G + I I+ + GE+E LRQIF A PSI+FIDE+D + P R+ A + V +R+++ LL ++GI + V+ + ATN LDAA+ R GR IEIG+P+ R IL
Sbjct: 355 IGGLSSQLKAIREIIELPLKQPELFKSYGIPAPRGVLLYGPPGTGKTMIARAVANEVGAYVSVINGPEIISKFYGETEAKLRQIFAEATLRHPSIIFIDELDALCPKREG-AQNEVEKRVVASLLTLMDGIGSEVSEGQVLVLGATNRPHALDAALRRPGRFDKEIEIGVPNAQDRLDIL 533
BLAST of AAA family ATPase vs. Ensembl Human
Match: VCP (valosin containing protein [Source:HGNC Symbol;Acc:HGNC:12666]) HSP 1 Score: 153.68 bits (387), Expect = 1.796e-37 Identity = 130/463 (28.08%), Postives = 217/463 (46.87%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-SNLNEEFH------------LIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF-----IDNVYRKFKEITLE----------RLKACRKDSNWI-----PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR--DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVE 1646
++++GP G GK + + +A+E FF IN L +E + K + A K+ ++ +++ + E+ H ++D L + + + + PNS RR +EV + I D R E+ + + M DD + ++ + N+ + G DL + + +K I LE L D W PS L + ++ + W DIGG E VK L+ ++ P+++ +F+K GVLF+GPPGC KT+ +A+A E N ISIK ++ + GESE +R+IFD+A P +LF DE+D I +R + + R+++++L ++G++ K NV + ATN D +D A++R GR+ I I LPD+ SR +IL A DV F +A+ +G SGA++ E
Sbjct: 241 ILLYGPPGTGKTLIARAVANET-GAFFFLINGPEIMSKLAGESESNLRKAFEE---AEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQI---HTKNMKLADDVDLEQVA-NETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSP----VAKDVDLEF--LAKMTNGFSGADLTE 689 HSP 2 Score: 140.969 bits (354), Expect = 2.147e-33 Identity = 106/373 (28.42%), Postives = 176/373 (47.18%), Query Frame = 3
Query: 2223 DLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA---------------QFHNNNENV--SDSLPTIKYQYFREAI 3263
DL+ V ++ G V + +E L+ + K+ I E I+ V N T D RW L S+++ L E E+ + W DIGG E VK L E ++ P++H G+ KG+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R I +A +++P ++F +++D + R + +R++++++T + G+ TKK NV I+ TN +D A+LRPGRL ++ I LPD+K R +ILK + ++ +A+ G SGA++ I + + A Q + + V D +P I+ +F EA+
Sbjct: 395 DLEQVANETHGHVGADLAALC---SEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWAL-------SQSNPSALRETVVEVPQVT-----------WEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKK--NVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKD----VDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAM 740 HSP 3 Score: 129.413 bits (324), Expect = 8.476e-30 Identity = 81/239 (33.89%), Postives = 125/239 (52.30%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRALPL 1691
L + + DIGG +K +E PL++ G + +G+L +GPPG KT+ RA+A ETG I IM GESE LR+ F+ A P+I+FIDE+D I P R+ V +R++S+LL ++G+ ++ +VI +AATN + +D A+ R GR ++IG+PD R IL I + ADDV + +A G GA++ + L +R+ + L
Sbjct: 198 LNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTH-GEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMK----LADDV--DLEQVANETHGHVGADLAALCSEAALQAIRKKMDL 429 HSP 4 Score: 126.331 bits (316), Expect = 7.593e-29 Identity = 72/227 (31.72%), Postives = 124/227 (54.63%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
+ DIGG + E +E+P++H G++ P+GIL +GPPG KT+ A+A E F + +IM + GESE+ R +A +++P++IFI+++D + PKR + V RI+S+L+T + G+ K+ +V+++A TN +++D AL R GR V IG+PD R IL+I ++ + ++ +A G GA++ + E +A
Sbjct: 203 YDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKR-EKTHGEVERRIVSQLLTLMDGL--KQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADD----VDLEQVANETHGHVGADLAALCSEAALQA 422
BLAST of AAA family ATPase vs. Ensembl Human
Match: SPATA5L1 (spermatogenesis associated 5 like 1 [Source:HGNC Symbol;Acc:HGNC:28762]) HSP 1 Score: 145.206 bits (365), Expect = 8.028e-35 Identity = 96/274 (35.04%), Postives = 144/274 (52.55%), Query Frame = 3
Query: 2517 QPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRS-SNSKSNVCERILSELMTFLSGIETKK-----------------NQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHNNNENVSDSLPTIKYQYFREAI 3263
+P+ W +IGG E VK L ++IE P+K GL PKG+L +GPPGC KT V ALAT C +V +D+ VG+SE I QA S+P+++F+++ID ++ RS S + +V ER+LS L+ L G+ K N++VMI+A TN +D ALLRPGRL I+ I PD K R SILK+ + + ++ +A SGA++ R + +A EN D+ T+K ++F +++
Sbjct: 459 KPVDWEEIGGLEDVKLKLKQSIEWPLKFPWEFVRMGLTQPKGVLLYGPPGCAKTTLVRALATSCHCSFVSVSGADLFSPFVGDSEKVLSQIFRQARASTPAILFLDEIDSILGARSASKTGCDVQERVLSVLLNELDGVGLKTIERRGSKSSQQEFQEVFNRSVMIIAATNRPDVLDTALLRPGRLDKIIYIPPPDHKGRLSILKVCTKTMPIGPD----VSLENLAAETCFFSGADL----RNLCTEAALLALQENGLDAT-TVKQEHFLKSL 723 HSP 2 Score: 135.961 bits (341), Expect = 6.254e-32 Identity = 89/222 (40.09%), Postives = 121/222 (54.50%), Query Frame = 3
Query: 960 NWIPSELLAYIA--DLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVS-PVSQRMMSELLMFLNGINEKP-------------------NVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGI 1538
N PS + I D++P+ W +IGG E VK+ LK IE PLK EFV KGVL +GPPGC+KT VRALAT + +S+ + + +VG+SE +L QIF +A A P+ILF+DEID I +R V +R++S LL L+G+ K +V+ +AATN D LD A++R GR+ I I PD R SIL +
Sbjct: 443 NIQPSSFRSVIGLMDIKPVDWEEIGGLEDVKLKLKQSIEWPLKFPWEFVRMGLTQPKGVLLYGPPGCAKTTLVRALATSCHCSFVSVSGADLFSPFVGDSEKVLSQIFRQARASTPAILFLDEIDSILGARSASKTGCDVQERVLSVLLNELDGVGLKTIERRGSKSSQQEFQEVFNRSVMIIAATNRPDVLDTALLRPGRLDKIIYIPPPDHKGRLSILKV 664 HSP 3 Score: 107.457 bits (267), Expect = 5.060e-23 Identity = 66/209 (31.58%), Postives = 108/209 (51.67%), Query Frame = 3
Query: 2601 GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALE---SSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHN--NNEN 3212
GL +P+G+L GPPG KT V A+A E ++ V + GE+E R + +A E PSL+F++++D L P+R S + + R++++++T L G ++ V++V TN +D AL RPGR V IG P K R+ IL++ S + + ++ +AE G GA++ + RE A H+ N +N
Sbjct: 230 GLAVPRGVLLAGPPGVGKTQLVRAVAREAGAELLAVSAPALQGSRPGETEENVRRVFQRARELASRGPSLLFLDEMDALCPQRGSRAPES---RVVAQVLTLLDG--ASGDREVVVVGATNRPDALDPALRRPGRFDREVVIGTPTLKQRKEILQVITSKMPISSH----VDLGLLAEMTVGYVGADLTALCREAAMHALLHSEKNQDN 429 HSP 4 Score: 101.679 bits (252), Expect = 3.511e-21 Identity = 58/157 (36.94%), Postives = 84/157 (53.50%), Query Frame = 3
Query: 1086 GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQ---PSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFS 1547
G +GVL GPPG KT VRA+A E G L+++ + GE+E +R++F RA L PS+LF+DE+D + P R A R+++++L L+G + V+ V ATN D LD A+ R GR + IG P R+ IL + S
Sbjct: 230 GLAVPRGVLLAGPPGVGKTQLVRAVAREAGAELLAVSAPALQGSRPGETEENVRRVFQRARELASRGPSLLFLDEMDALCPQRGSRA---PESRVVAQVLTLLDGASGDREVVVVGATNRPDALDPALRRPGRFDREVVIGTPTLKQRKEILQVITS 383
BLAST of AAA family ATPase vs. Ensembl Human
Match: NVL (nuclear VCP like [Source:HGNC Symbol;Acc:HGNC:8070]) HSP 1 Score: 137.502 bits (345), Expect = 2.233e-32 Identity = 80/231 (34.63%), Postives = 121/231 (52.38%), Query Frame = 3
Query: 1014 WVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAE--RLSGKSGAEIVEFGRQVRLGVRR 1679
W DIG E ++ L I P++N +F GVL GPPGC KT+ +A+A E+GLN IS+K ++ YVGESE +RQ+F RA P ++F DE+D + P R S R++++LL ++G+ + V +AATN D +D A++R GR+ + +GLP R +IL + + AD N AIA R +GA++ R+ + R
Sbjct: 474 WADIGALEDIREELTMAILAPVRNPDQFKALGLVTPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRAKNSAPCVIFFDEVDALCPRRSDRETG-ASVRVVNQLLTEMDGLEARQQVFIMAATNRPDIIDPAILRPGRLDKTLFVGLPPPADRLAILKTITKNGTKPPLDAD---VNLEAIAGDLRCDCYTGADLSALVREASICALR 700 HSP 2 Score: 133.265 bits (334), Expect = 3.949e-31 Identity = 84/257 (32.68%), Postives = 135/257 (52.53%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLT--GLSGAEIVGIVREMK----RKAQFHNNNENVSDSLPTIKYQYFREA 3260
W DIG E ++ L I P+++ GL P G+L GPPGC KT+ A+A E L +V +++ VGESE A R + +A S+P +IF +++D L P+R S+ ++ R++++L+T + G+E + Q V I+A TN +D A+LRPGRL + +GLP DR +ILK + + + N+ IA L +GA++ +VRE R+ + N L + +++F EA
Sbjct: 474 WADIGALEDIREELTMAILAPVRNPDQFKALGLVTPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRAKNSAPCVIFFDEVDALCPRR-SDRETGASVRVVNQLLTEMDGLEAR--QQVFIMAATNRPDIIDPAILRPGRLDKTLFVGLPPPADRLAILKT-ITKNGTKPPLDADVNLEAIAGDLRCDCYTGADLSALVREASICALRQEMARQKSGNEKGEL-KVSHKHFEEA 725 HSP 3 Score: 127.487 bits (319), Expect = 2.566e-29 Identity = 80/263 (30.42%), Postives = 138/263 (52.47%), Query Frame = 3
Query: 2415 LKSASVKYSRTDMRQL---IEFSREIENTSKELDHQSQPLHWTDIGG----YETVKTMLIENIEMPIKHGLEI--PKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGI-ETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
LK+ K + D++++ IE + + ++ L+ Q + + D+GG + V MLI + H L + P+G+L HGPPGC KT+ +A+A E L + V +I+ G+ GESE R + QA+ ++P +IFI++ID + PKR SK ++ RI+++L+T + + V+++ TN ++D AL R GR + +G+PD+ RE IL+ L + +A G GA+++ + RE
Sbjct: 117 LKNKGSKRKKEDLQEVDGEIEAVLQKKAKARGLEFQISNVKFEDVGGNDMTLKEVCKMLIHMRHPEVYHHLGVVPPRGVLLHGPPGCGKTLLAHAIAGELDLPILKVAAPEIVSGVSGESEQKLRELFEQAVSNAPCIIFIDEIDAITPKREVASK-DMERRIVAQLLTCMDDLNNVAATARVLVIGATNRPDSLDPALRRAGRFDREICLGIPDEASRERILQTLCRKLRLPQAFDFCH----LAHLTPGFVGADLMALCRE 374 HSP 4 Score: 123.635 bits (309), Expect = 5.263e-28 Identity = 63/147 (42.86%), Postives = 92/147 (62.59%), Query Frame = 3
Query: 1101 KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMF---LNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
+GVL HGPPGC KT+ A+A E L ++ + I+ G GESE LR++F++AV+ P I+FIDEID I P R+ +A + +R++++LL LN + V+ + ATN D LD A+ R+GR I +G+PD+ SRE IL
Sbjct: 193 RGVLLHGPPGCGKTLLAHAIAGELDLPILKVAAPEIVSGVSGESEQKLRELFEQAVSNAPCIIFIDEIDAITPKRE-VASKDMERRIVAQLLTCMDDLNNVAATARVLVIGATNRPDSLDPALRRAGRFDREICLGIPDEASRERIL 338
BLAST of AAA family ATPase vs. Ensembl Human
Match: NVL (nuclear VCP like [Source:HGNC Symbol;Acc:HGNC:8070]) HSP 1 Score: 136.732 bits (343), Expect = 2.306e-32 Identity = 80/231 (34.63%), Postives = 121/231 (52.38%), Query Frame = 3
Query: 1014 WVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAE--RLSGKSGAEIVEFGRQVRLGVRR 1679
W DIG E ++ L I P++N +F GVL GPPGC KT+ +A+A E+GLN IS+K ++ YVGESE +RQ+F RA P ++F DE+D + P R S R++++LL ++G+ + V +AATN D +D A++R GR+ + +GLP R +IL + + AD N AIA R +GA++ R+ + R
Sbjct: 391 WADIGALEDIREELTMAILAPVRNPDQFKALGLVTPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRAKNSAPCVIFFDEVDALCPRRSDRETG-ASVRVVNQLLTEMDGLEARQQVFIMAATNRPDIIDPAILRPGRLDKTLFVGLPPPADRLAILKTITKNGTKPPLDAD---VNLEAIAGDLRCDCYTGADLSALVREASICALR 617 HSP 2 Score: 133.265 bits (334), Expect = 2.465e-31 Identity = 84/257 (32.68%), Postives = 135/257 (52.53%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLT--GLSGAEIVGIVREMK----RKAQFHNNNENVSDSLPTIKYQYFREA 3260
W DIG E ++ L I P+++ GL P G+L GPPGC KT+ A+A E L +V +++ VGESE A R + +A S+P +IF +++D L P+R S+ ++ R++++L+T + G+E + Q V I+A TN +D A+LRPGRL + +GLP DR +ILK + + + N+ IA L +GA++ +VRE R+ + N L + +++F EA
Sbjct: 391 WADIGALEDIREELTMAILAPVRNPDQFKALGLVTPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRAKNSAPCVIFFDEVDALCPRR-SDRETGASVRVVNQLLTEMDGLEAR--QQVFIMAATNRPDIIDPAILRPGRLDKTLFVGLPPPADRLAILKT-ITKNGTKPPLDADVNLEAIAGDLRCDCYTGADLSALVREASICALRQEMARQKSGNEKGEL-KVSHKHFEEA 642 HSP 3 Score: 114.39 bits (285), Expect = 2.493e-25 Identity = 81/290 (27.93%), Postives = 139/290 (47.93%), Query Frame = 3
Query: 2415 LKSASVKYSRTDMRQL---IEFSREIENTSKELDHQSQPLHWTDIGG----YETVKTMLIENIEMPIKHGLEI--PKGILFHGPPGCPKTMFVNALA-TECQ--------------------------LKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGI-ETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
LK+ K + D++++ IE + + ++ L+ Q + + D+GG + V MLI + H L + P+G+L HGPPGC KT+ +A+A EC L + V +I+ G+ GESE R + QA+ ++P +IFI++ID + PKR SK ++ RI+++L+T + + V+++ TN ++D AL R GR + +G+PD+ RE IL+ L + +A G GA+++ + RE
Sbjct: 7 LKNKGSKRKKEDLQEVDGEIEAVLQKKAKARGLEFQISNVKFEDVGGNDMTLKEVCKMLIHMRHPEVYHHLGVVPPRGVLLHGPPGCGKTLLAHAIAGAECSGMITAHCSFDFSGSNDPPASASQELDLPILKVAAPEIVSGVSGESEQKLRELFEQAVSNAPCIIFIDEIDAITPKREVASK-DMERRIVAQLLTCMDDLNNVAATARVLVIGATNRPDSLDPALRRAGRFDREICLGIPDEASRERILQTLCRKLRLPQAFDFCH----LAHLTPGFVGADLMALCRE 291 HSP 4 Score: 108.612 bits (270), Expect = 1.656e-23 Identity = 63/174 (36.21%), Postives = 92/174 (52.87%), Query Frame = 3
Query: 1101 KGVLFHGPPGCSKTMFVRALA---------------------------TETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMF---LNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
+GVL HGPPGC KT+ A+A E L ++ + I+ G GESE LR++F++AV+ P I+FIDEID I P R+ +A + +R++++LL LN + V+ + ATN D LD A+ R+GR I +G+PD+ SRE IL
Sbjct: 83 RGVLLHGPPGCGKTLLAHAIAGAECSGMITAHCSFDFSGSNDPPASASQELDLPILKVAAPEIVSGVSGESEQKLRELFEQAVSNAPCIIFIDEIDAITPKRE-VASKDMERRIVAQLLTCMDDLNNVAATARVLVIGATNRPDSLDPALRRAGRFDREICLGIPDEASRERIL 255
BLAST of AAA family ATPase vs. Ensembl Celegans
Match: cdc-48.3 (ATPase family protein 2 homolog [Source:UniProtKB/Swiss-Prot;Acc:Q21222]) HSP 1 Score: 171.785 bits (434), Expect = 6.627e-44 Identity = 98/313 (31.31%), Postives = 172/313 (54.95%), Query Frame = 3
Query: 2364 ELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHNNNENVSDSLPTIKYQYFREAISASCS 3278
++ ++ HGFT D+ LK+A RT + ++ + + I T ++ + + W DIGG E +K + + + P KH G++ P GIL +GPPGC KT+ ALA+E ++ V ++ VG+SE A R + S+A + +P+++F ++ID + R S S V +R+L++L+T L G+E K+ V+++A TN +D ALLRPGRL + +GLP + R +IL++ + F + + I + E +G SGAE+V + R A E++ ++ +++ +F +A++A S
Sbjct: 402 DVARHTHGFTGGDLCSLLKAAKFARGRTHLERVNDARKRIRPTGIRQFILEVPNVSWNDIGGNEELKLEIQQAVIWPQKHPEAFERFGIDPPAGILLYGPPGCSKTLIARALASEAKMNFLAVKGPELFSKWVGDSEKAIRDLFSRARQVAPTIVFFDEIDAVGSSRGSEKSSGVSDRVLAQLLTELDGLE--KSSRVILLAATNRPDQLDSALLRPGRLDRAIYVGLPCEVTRRAILEMRTKKMKFD---DTVRTIDKLVEKTSGYSGAELVAVC----RTAAMFAMRESIDATI--VQWTHFEQALAAVVS 703 HSP 2 Score: 169.859 bits (429), Expect = 2.851e-43 Identity = 98/279 (35.13%), Postives = 154/279 (55.20%), Query Frame = 3
Query: 864 FTGKDLTDFIDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRR 1679
FTG DL + LER+ RK P+ + +I ++ + W DIGG E +K+ ++ + P K+ + F + G+L +GPPGCSKT+ RALA+E +N +++K + +VG+SE +R +F RA + P+I+F DEID + SR S VS R++++LL L+G+ + VI +AATN D+LD+A++R GR+ I +GLP +V+R +IL + + F DD + E+ SG SGAE+V R + R
Sbjct: 410 FTGGDLCSLLKAAKFARGRTHLERVNDARKRIR--PTGIRQFILEVPNVSWNDIGGNEELKLEIQQAVIWPQKHPEAFERFGIDPPAGILLYGPPGCSKTLIARALASEAKMNFLAVKGPELFSKWVGDSEKAIRDLFSRARQVAPTIVFFDEIDAVGSSRGSEKSSGVSDRVLAQLLTELDGLEKSSRVILLAATNRPDQLDSALLRPGRLDRAIYVGLPCEVTRRAILEM----RTKKMKF-DDTVRTIDKLVEKTSGYSGAELVAVCRTAAMFAMR 681
BLAST of AAA family ATPase vs. Ensembl Celegans
Match: cdc-48.2 (Transitional endoplasmic reticulum ATPase homolog 2 [Source:UniProtKB/Swiss-Prot;Acc:P54812]) HSP 1 Score: 149.058 bits (375), Expect = 2.374e-36 Identity = 130/484 (26.86%), Postives = 223/484 (46.07%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-SNLNEEFH------------LIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLT----------------------DFIDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLA--VSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEF-GRQVRLGVRRALPLDTR 1700
+++ GP G GK + + +A+E FF IN ++ +E + K + K+ +L I++ + E+ H ++D L + + + PNS RR +E+ + I D R E+ + + M +D + ++ + N+ + F G DL D ID +T+E + S+ PS L + + W DIGG ++VK L+ ++ P+++ ++++K GVLF+GPPGC KT+ +A+A E N ISIK ++ + GESE +R +FD+A A P +LF DE+D I +R + R+++++L ++G+N K NV + ATN D +D AV+R GR+ I I LPD+ SR I + + D+ NF +A+ G SGA++ E R +L +R ++ + R
Sbjct: 246 ILLFGPPGTGKTLIARAVANET-GAFFFLINGPEIMSKMSGESESNLRKAFAE---CEKNSPAILFIDEIDAIAPKREKAHGEVEKRIVSQLLTLMDGLKTRAHVVVIAATNRPNSIDGALRRFGRFDREIDIGIPDAVGRLEILRI---HTKNMKLGEDVDLEQVA-NECHGFVGADLASLCSEAAIQQIREKMELIDLEDDTIDAEVLNSLAVTMENFRFAMGKSS--PSALREAVVETPNTTWSDIGGLQNVKRELQELVQYPVEHPEKYLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDVFDKARAAAPCVLFFDELDSIAKARGGSVGDAGGAADRVINQVLTEMDGMNAKKNVFIIGATNRPDIIDPAVLRPGRLDQLIYIPLPDEASRLQIFKASLRKTP----LSADLDLNF--LAKNTVGFSGADLTEICQRACKLAIRESIEREIR 713 HSP 2 Score: 140.584 bits (353), Expect = 1.014e-33 Identity = 92/357 (25.77%), Postives = 171/357 (47.90%), Query Frame = 3
Query: 2355 DIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSRE-------------IEN-----------TSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIV-------------GIVREMKRKAQFHNNN--------ENVSDSLPTIKYQYFREAI 3263
D+E++ HGF D+ A+++ R M +LI+ + +EN +E ++ W+DIGG + VK L E ++ P++H G++ +G+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R + +A ++P ++F +++D + R S +R++++++T + G+ KK NV I+ TN +D A+LRPGRL ++ I LPD+ R I K + +++ +A++ G SGA++ I RE++++ + + + + ++D +P I +F EA+
Sbjct: 400 DLEQVANECHGFVGADLASLCSEAAIQQIREKM-ELIDLEDDTIDAEVLNSLAVTMENFRFAMGKSSPSALREAVVETPNTTWSDIGGLQNVKRELQELVQYPVEHPEKYLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDVFDKARAAAPCVLFFDELDSIAKARGGSVGDAGGAADRVINQVLTEMDGMNAKK--NVFIIGATNRPDIIDPAVLRPGRLDQLIYIPLPDEASRLQIFKASLRKTPLSADL----DLNFLAKNTVGFSGADLTEICQRACKLAIRESIEREIRQEKERQDRSARGEELMEDELADPVPEITRAHFEEAM 749 HSP 3 Score: 124.02 bits (310), Expect = 1.579e-28 Identity = 72/187 (38.50%), Postives = 103/187 (55.08%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGI 1538
L + + D+GG +K +E PL++ + F +G+L GPPG KT+ RA+A ETG I IM GESE LR+ F P+ILFIDEID I P R+ A V +R++S+LL ++G+ + +V+ +AATN + +D A+ R GR I+IG+PD V R IL I
Sbjct: 203 LNEVGYDDLGGVRKQLAQIKEMVELPLRHPQLFKAIGVKPPRGILLFGPPGTGKTLIARAVANETGAFFFLINGPEIMSKMSGESESNLRKAFAECEKNSPAILFIDEIDAIAPKREK-AHGEVEKRIVSQLLTLMDGLKTRAHVVVIAATNRPNSIDGALRRFGRFDREIDIGIPDAVGRLEILRI 388 HSP 4 Score: 123.25 bits (308), Expect = 2.771e-28 Identity = 69/222 (31.08%), Postives = 123/222 (55.41%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
+ D+GG + E +E+P++H G++ P+GIL GPPG KT+ A+A E F + +IM + GESE+ R ++ ++SP+++FI++ID + PKR + V +RI+S+L+T + G++T+ +V+++A TN +++D AL R GR + IG+PD R IL+I ++ + ++ +A G GA++ + E
Sbjct: 208 YDDLGGVRKQLAQIKEMVELPLRHPQLFKAIGVKPPRGILLFGPPGTGKTLIARAVANETGAFFFLINGPEIMSKMSGESESNLRKAFAECEKNSPAILFIDEIDAIAPKR-EKAHGEVEKRIVSQLLTLMDGLKTR--AHVVVIAATNRPNSIDGALRRFGRFDREIDIGIPDAVGRLEILRIHTKNMKLGED----VDLEQVANECHGFVGADLASLCSE 422
BLAST of AAA family ATPase vs. Ensembl Celegans
Match: cdc-48.2 (Transitional endoplasmic reticulum ATPase homolog 2 [Source:UniProtKB/Swiss-Prot;Acc:P54812]) HSP 1 Score: 149.058 bits (375), Expect = 2.374e-36 Identity = 130/484 (26.86%), Postives = 223/484 (46.07%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-SNLNEEFH------------LIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLT----------------------DFIDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLA--VSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEF-GRQVRLGVRRALPLDTR 1700
+++ GP G GK + + +A+E FF IN ++ +E + K + K+ +L I++ + E+ H ++D L + + + PNS RR +E+ + I D R E+ + + M +D + ++ + N+ + F G DL D ID +T+E + S+ PS L + + W DIGG ++VK L+ ++ P+++ ++++K GVLF+GPPGC KT+ +A+A E N ISIK ++ + GESE +R +FD+A A P +LF DE+D I +R + R+++++L ++G+N K NV + ATN D +D AV+R GR+ I I LPD+ SR I + + D+ NF +A+ G SGA++ E R +L +R ++ + R
Sbjct: 246 ILLFGPPGTGKTLIARAVANET-GAFFFLINGPEIMSKMSGESESNLRKAFAE---CEKNSPAILFIDEIDAIAPKREKAHGEVEKRIVSQLLTLMDGLKTRAHVVVIAATNRPNSIDGALRRFGRFDREIDIGIPDAVGRLEILRI---HTKNMKLGEDVDLEQVA-NECHGFVGADLASLCSEAAIQQIREKMELIDLEDDTIDAEVLNSLAVTMENFRFAMGKSS--PSALREAVVETPNTTWSDIGGLQNVKRELQELVQYPVEHPEKYLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDVFDKARAAAPCVLFFDELDSIAKARGGSVGDAGGAADRVINQVLTEMDGMNAKKNVFIIGATNRPDIIDPAVLRPGRLDQLIYIPLPDEASRLQIFKASLRKTP----LSADLDLNF--LAKNTVGFSGADLTEICQRACKLAIRESIEREIR 713 HSP 2 Score: 140.584 bits (353), Expect = 1.014e-33 Identity = 92/357 (25.77%), Postives = 171/357 (47.90%), Query Frame = 3
Query: 2355 DIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSRE-------------IEN-----------TSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIV-------------GIVREMKRKAQFHNNN--------ENVSDSLPTIKYQYFREAI 3263
D+E++ HGF D+ A+++ R M +LI+ + +EN +E ++ W+DIGG + VK L E ++ P++H G++ +G+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R + +A ++P ++F +++D + R S +R++++++T + G+ KK NV I+ TN +D A+LRPGRL ++ I LPD+ R I K + +++ +A++ G SGA++ I RE++++ + + + + ++D +P I +F EA+
Sbjct: 400 DLEQVANECHGFVGADLASLCSEAAIQQIREKM-ELIDLEDDTIDAEVLNSLAVTMENFRFAMGKSSPSALREAVVETPNTTWSDIGGLQNVKRELQELVQYPVEHPEKYLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDVFDKARAAAPCVLFFDELDSIAKARGGSVGDAGGAADRVINQVLTEMDGMNAKK--NVFIIGATNRPDIIDPAVLRPGRLDQLIYIPLPDEASRLQIFKASLRKTPLSADL----DLNFLAKNTVGFSGADLTEICQRACKLAIRESIEREIRQEKERQDRSARGEELMEDELADPVPEITRAHFEEAM 749 HSP 3 Score: 124.02 bits (310), Expect = 1.579e-28 Identity = 72/187 (38.50%), Postives = 103/187 (55.08%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGI 1538
L + + D+GG +K +E PL++ + F +G+L GPPG KT+ RA+A ETG I IM GESE LR+ F P+ILFIDEID I P R+ A V +R++S+LL ++G+ + +V+ +AATN + +D A+ R GR I+IG+PD V R IL I
Sbjct: 203 LNEVGYDDLGGVRKQLAQIKEMVELPLRHPQLFKAIGVKPPRGILLFGPPGTGKTLIARAVANETGAFFFLINGPEIMSKMSGESESNLRKAFAECEKNSPAILFIDEIDAIAPKREK-AHGEVEKRIVSQLLTLMDGLKTRAHVVVIAATNRPNSIDGALRRFGRFDREIDIGIPDAVGRLEILRI 388 HSP 4 Score: 123.25 bits (308), Expect = 2.771e-28 Identity = 69/222 (31.08%), Postives = 123/222 (55.41%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
+ D+GG + E +E+P++H G++ P+GIL GPPG KT+ A+A E F + +IM + GESE+ R ++ ++SP+++FI++ID + PKR + V +RI+S+L+T + G++T+ +V+++A TN +++D AL R GR + IG+PD R IL+I ++ + ++ +A G GA++ + E
Sbjct: 208 YDDLGGVRKQLAQIKEMVELPLRHPQLFKAIGVKPPRGILLFGPPGTGKTLIARAVANETGAFFFLINGPEIMSKMSGESESNLRKAFAECEKNSPAILFIDEIDAIAPKR-EKAHGEVEKRIVSQLLTLMDGLKTR--AHVVVIAATNRPNSIDGALRRFGRFDREIDIGIPDAVGRLEILRIHTKNMKLGED----VDLEQVANECHGFVGADLASLCSE 422
BLAST of AAA family ATPase vs. Ensembl Celegans
Match: cdc-48.1 (Transitional endoplasmic reticulum ATPase homolog 1 [Source:UniProtKB/Swiss-Prot;Acc:P54811]) HSP 1 Score: 147.132 bits (370), Expect = 9.026e-36 Identity = 111/372 (29.84%), Postives = 179/372 (48.12%), Query Frame = 3
Query: 696 PNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLT----------------------DFIDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMI---FPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEF-GRQVRLGVRRALPLDTR 1700
PNS RR +E+ + I D R E+ + + M DD + ++ + N+ + F G DL D ID +T+E + + S+ PS L + + W DIGG ++VK L+ ++ P+++ ++++K GVLF+GPPGC KT+ +A+A E N ISIK ++ + GESE +R +FD+A A P +LF DE+D I S R+++++L ++G+N K NV + ATN D +D AV+R GR+ I I LPD+ SR IL + + D+ F +A+ G SGA++ E R +L +R ++ + R
Sbjct: 356 PNSIDGALRRFGRFDREIDIGIPDAVGRLEILRI---HTKNMKLADDVDLEQIA-NECHGFVGADLASLCSEAALQQIREKMELIDLEDDQIDAEVLNSLAVTMENFRFAQGKSS--PSALREAVVETPNTTWSDIGGLQNVKRELQELVQYPVEHPEKYLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDVFDKARAAAPCVLFFDELDSIAKARGGGAGGDGGGASDRVINQVLTEMDGMNAKKNVFIIGATNRPDIIDPAVLRPGRLDQLIYIPLPDEASRHQILKASLRKTP----LSKDLDLTF--LAKNTVGFSGADLTEICQRACKLAIRESIEKEIR 715 HSP 2 Score: 132.88 bits (333), Expect = 2.917e-31 Identity = 92/358 (25.70%), Postives = 167/358 (46.65%), Query Frame = 3
Query: 2355 DIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSRE-------------IEN-----------TSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLL---IPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVR-----------------EMKRKAQFHNNNE----NVSDSLPTIKYQYFREAI 3263
D+E++ HGF D+ A+++ R M +LI+ + +EN +E ++ W+DIGG + VK L E ++ P++H G++ +G+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R + +A ++P ++F +++D + + +R++++++T + G+ KK NV I+ TN +D A+LRPGRL ++ I LPD+ R ILK + +++ +A++ G SGA++ I + E +R+ + E + D +P I +F EA+
Sbjct: 401 DLEQIANECHGFVGADLASLCSEAALQQIREKM-ELIDLEDDQIDAEVLNSLAVTMENFRFAQGKSSPSALREAVVETPNTTWSDIGGLQNVKRELQELVQYPVEHPEKYLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDVFDKARAAAPCVLFFDELDSIAKARGGGAGGDGGGASDRVINQVLTEMDGMNAKK--NVFIIGATNRPDIIDPAVLRPGRLDQLIYIPLPDEASRHQILKASLRKTPLSKDL----DLTFLAKNTVGFSGADLTEICQRACKLAIRESIEKEIRIEKERQDRQARGEELMEDDAVDPVPEITRAHFEEAM 751 HSP 3 Score: 129.028 bits (323), Expect = 4.185e-30 Identity = 80/227 (35.24%), Postives = 117/227 (51.54%), Query Frame = 3
Query: 1008 LHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
+ + D+GG +K +E PL++ + F +G+L GPPG KT+ RA+A ETG I +M GESE LR+ F+ QP+ILFIDEID I P R+ V +R++S+LL ++G+ + N++ +AATN + +D A+ R GR I+IG+PD V R IL I ADDV + IA G GA++ + L
Sbjct: 207 IGYDDLGGVRKQLAQIKEMVELPLRHPQLFKAIGIKPPRGILLFGPPGTGKTLIARAVANETGSFFFLINGPEVMSKMSGESESNLRKAFEECEKNQPAILFIDEIDAIAPKREKTN-GEVERRIVSQLLTLMDGVKGRSNLVVIAATNRPNSIDGALRRFGRFDREIDIGIPDAVGRLEILRI----HTKNMKLADDV--DLEQIANECHGFVGADLASLCSEAAL 426 HSP 4 Score: 120.168 bits (300), Expect = 2.547e-27 Identity = 68/224 (30.36%), Postives = 119/224 (53.12%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
+ + D+GG + E +E+P++H G++ P+GIL GPPG KT+ A+A E F + ++M + GESE+ R + ++ P+++FI++ID + PKR + V RI+S+L+T + G+ K N++++A TN +++D AL R GR + IG+PD R IL+I ++ + ++ IA G GA++ + E
Sbjct: 207 IGYDDLGGVRKQLAQIKEMVELPLRHPQLFKAIGIKPPRGILLFGPPGTGKTLIARAVANETGSFFFLINGPEVMSKMSGESESNLRKAFEECEKNQPAILFIDEIDAIAPKR-EKTNGEVERRIVSQLLTLMDGV--KGRSNLVVIAATNRPNSIDGALRRFGRFDREIDIGIPDAVGRLEILRIHTKNMKLADD----VDLEQIANECHGFVGADLASLCSE 423
BLAST of AAA family ATPase vs. Ensembl Celegans
Match: mac-1 (pep chromosome:WBcel235:II:13311459:13320911:-1 gene:WBGene00003119.1 transcript:Y48C3A.7.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mac-1) HSP 1 Score: 132.494 bits (332), Expect = 3.110e-31 Identity = 89/268 (33.21%), Postives = 143/268 (53.36%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIKHGLEI--------PKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFS---------DLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE---MKRKAQFHNNNENVSDSLPTIKYQYFREAIS 3266
+ W DIG V+ L +I PIK + P+GIL GPPGC KT+ A+A E + +V +++ VGESE A R + +A +S P +IF ++ID L+PKR S+ +S+ R++++L+T + G+E + Q V ++ TN VD A+LRPGRL I+ + P +DR IL+ D++F HE I+ + E L G +GA++ ++ E + +A+ N+E+V + ++FREA S
Sbjct: 530 VSWDDIGALVEVRKQLEWSILYPIKRADDFAALGIDCRPQGILLCGPPGCGKTLLAKAVANETGMNFISVKGPELLNMYVGESERAVRTVFQRARDSQPCVIFFDEIDALVPKR-SHGESSGGARLVNQLLTEMDGVEGR--QKVFLIGATNRPDIVDAAILRPGRLDKILFVDFPSVEDRVDILRKSTKNGTRPMLGEDIDF---HE----IAQLPE-LAGFTGADLAALIHESSLLALQARVLENDESVK----GVGMRHFREAAS 782 HSP 2 Score: 131.724 bits (330), Expect = 6.067e-31 Identity = 95/328 (28.96%), Postives = 160/328 (48.78%), Query Frame = 3
Query: 774 LFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDFIDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV--------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAE--RLSGKSGAEIVEFGRQVRLGVRRALPLDTRYTSSGILIR 1727
+F ++ ++ G N + K+ D L G D + + +I+ E + R S P+ A + + W DIG V+ L+ I P+K +F +G+L GPPGC KT+ +A+A ETG+N IS+K ++ YVGESE +R +F RA QP ++F DEID + P R H S R++++LL ++G+ + V + ATN D +DAA++R GR+ + + P R IL + + + +D+ +F IA+ L+G +GA++ + L +A L+ + G+ +R
Sbjct: 455 VFDTIVVKNEGHKNLTVEQIKEELDRVLAWLQGDDDPSALSELNGGL-QISFEDFE--RALSTIQPAAKREGFATVPDVSWDDIGALVEVRKQLEWSILYPIKRADDFAALGIDCRPQGILLCGPPGCGKTLLAKAVANETGMNFISVKGPELLNMYVGESERAVRTVFQRARDSQPCVIFFDEIDALVPKRSH-GESSGGARLVNQLLTEMDGVEGRQKVFLIGATNRPDIVDAAILRPGRLDKILFVDFPSVEDRVDILRKSTKNGTRP-MLGEDI--DFHEIAQLPELAGFTGADLAALIHESSLLALQARVLENDESVKGVGMR 775 HSP 3 Score: 102.449 bits (254), Expect = 7.944e-22 Identity = 61/222 (27.48%), Postives = 108/222 (48.65%), Query Frame = 3
Query: 2601 GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSK-------SNVC-----------ERILSELMTF--------LSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
G++ P+G + HGPPGC KTMF A+A E + M + ++++ G+ GE+E R + A ++SP ++ ++ ID + P+R + + S +C E+ L + +TF + T V+++ TT+ VD L R GR + +G+PD+ RE IL+ +N + + IA+ G GA++ ++RE + A
Sbjct: 235 GVDPPRGFIVHGPPGCGKTMFAQAVAGELAIPMLQLAATELVSGVSGETEEKIRRLFDTAKQNSPCILILDDIDAIAPRRETAQREMERRVVSQLCSSLDELVLPPREKPLKDQLTFGDDGSVAIIGDSPTAAGAGVLVIGTTSRPDAVDGGLRRAGRFENEISLGIPDETAREKILE-KICKVNLAGD----VTLKQIAKLTPGYVGADLQALIREAAKVA 451 HSP 4 Score: 95.5153 bits (236), Expect = 1.138e-19 Identity = 53/172 (30.81%), Postives = 86/172 (50.00%), Query Frame = 3
Query: 1101 KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR-------------------DHLAVSPVSQRMMSELLMFLNG----INEKP-----NVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
+G + HGPPGC KTMF +A+A E + ++ + + ++ G GE+E +R++FD A P IL +D+ID I P R D L + P + + +L +G I + P V+ + T+ D +D + R+GR I +G+PD+ +RE IL
Sbjct: 240 RGFIVHGPPGCGKTMFAQAVAGELAIPMLQLAATELVSGVSGETEEKIRRLFDTAKQNSPCILILDDIDAIAPRRETAQREMERRVVSQLCSSLDELVLPPREKPLKDQLTFGDDGSVAIIGDSPTAAGAGVLVIGTTSRPDAVDGGLRRAGRFENEISLGIPDETAREKIL 411
BLAST of AAA family ATPase vs. Ensembl Fly
Match: TER94 (gene:FBgn0286784 transcript:FBtr0112906) HSP 1 Score: 157.918 bits (398), Expect = 3.122e-39 Identity = 135/483 (27.95%), Postives = 224/483 (46.38%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-------------SNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKF----------------SDNQLNQFTGK-DLTDF----IDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLA--VSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEF-GRQVRLGVRRALPLDTR 1700
++M+GP G GK + + +A+E FF IN L +E + K + A K+ ++ I++ + + ++D + ++S + + PNS RR +E+ + I D R E+ + + M DD + ++ S+ L Q K DL D ID +T+E + S+ PS L + ++ W DIGG ESVK L+ ++ P+++ +F+K GVLF+GPPGC KT+ +A+A E N IS+K ++ + GESE +R IFD+A + P +LF DE+D I +R + R+++++L ++G+ K NV + ATN D +D A++R GR+ I I LPD SRE+IL A +V + + IA+ G SGA++ E R +L +R+A+ + R
Sbjct: 196 ILMYGPPGTGKTLIARAVANET-GAFFFLINGPEIMSKLAGESESNLRKAFEE---AEKNSPAIIFIDEIDAIAPKRDKTHGEVERRIVSQLLTLMDGMKKSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRI---HTKNMKLHDDVDLEQIAAESHGHVGADLASLCSEAALQQIREKMDLIDLEDDKIDAEVLASLAVTMENFRYAMTKSS--PSALRETVVEVPNTTWTDIGGLESVKKELQELVQYPVEHPDKFLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISVKGPELLTMWFGESEANVRDIFDKARSAAPCVLFFDELDSIAKARGGNVGDAGGAADRVINQILTEMDGMGAKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDDKSREAILKANLRKSP----LAKEV--DLTYIAKVTQGFSGADLTEICQRACKLAIRQAIEAEIR 663 HSP 2 Score: 153.68 bits (387), Expect = 7.663e-38 Identity = 128/478 (26.78%), Postives = 212/478 (44.35%), Query Frame = 3
Query: 1992 EHLESTMQQTIFIRLSDKLATKEISEVIEVINHGNSKRK---DMVVVVDGMRKMSDDFCEAVQLTLINEAPNVKVMMIFEDLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIY-KILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRT------------DMRQLIEFSREIENTSKELDHQSQPL-----------HWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSN--SKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGI----------------VREMKRKAQFHNNNENV--SDSLPTIKYQYFREAI 3263
E E IFI D +A K HG +R+ ++ ++DGM+K S LI A + I L+ G ++++ + +P T I+ K + + V D+E++ HG D+ A+++ R D L + +EN + S WTDIGG E+VK L E ++ P++H G++ +G+LF+GPPGC KT+ A+A ECQ +V +++ GESEA R I +A ++P ++F +++D + R N +R++++++T + G+ KK NV I+ TN +D A+LRPGRL ++ I LPD K RE+ILK E +++ IA+ G SGA++ I +R K +A+ N+ ++ D +P I +F EA+
Sbjct: 246 EEAEKNSPAIIFIDEIDAIAPKRDK------THGEVERRIVSQLLTLMDGMKKSSH---------LIVMAATNRPNSIDPALRRF-----GRFDREIDIGIPDATGRLEVLRIHTKNMKLHDDV-DLEQIAAESHGHVGADLASLCSEAALQQIREKMDLIDLEDDKIDAEVLASLAVTMENFRYAMTKSSPSALRETVVEVPNTTWTDIGGLESVKKELQELVQYPVEHPDKFLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISVKGPELLTMWFGESEANVRDIFDKARSAAPCVLFFDELDSIAKARGGNVGDAGGAADRVINQILTEMDGMGAKK--NVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDDKSREAILKANLRKSPLAKE----VDLTYIAKVTQGFSGADLTEICQRACKLAIRQAIEAEIRREKERAENQNSAMDMDEDDPVPEITSAHFEEAM 696 HSP 3 Score: 127.102 bits (318), Expect = 2.048e-29 Identity = 73/222 (32.88%), Postives = 125/222 (56.31%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
+ DIGG + E +E+P++H G++ P+GIL +GPPG KT+ A+A E F + +IM + GESE+ R +A ++SP++IFI++ID + PKR + V RI+S+L+T + G+ KK+ +++++A TN +++D AL R GR + IG+PD R +L+I ++ H+ + ++ IA G GA++ + E
Sbjct: 158 YDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPPRGILMYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNSPAIIFIDEIDAIAPKR-DKTHGEVERRIVSQLLTLMDGM--KKSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRIHTKNMKL---HDDV-DLEQIAAESHGHVGADLASLCSE 372 HSP 4 Score: 126.331 bits (316), Expect = 3.192e-29 Identity = 80/230 (34.78%), Postives = 118/230 (51.30%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
L + + DIGG +K +E PL++ G + +G+L +GPPG KT+ RA+A ETG I IM GESE LR+ F+ A P+I+FIDEID I P RD V +R++S+LL ++G+ + ++I +AATN + +D A+ R GR I+IG+PD R +L I + DDV + IA G GA++ + L
Sbjct: 153 LNAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPPRGILMYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNSPAIIFIDEIDAIAPKRDKTH-GEVERRIVSQLLTLMDGMKKSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRIHTKNMK----LHDDV--DLEQIAAESHGHVGADLASLCSEAAL 375
BLAST of AAA family ATPase vs. Ensembl Fly
Match: TER94 (gene:FBgn0286784 transcript:FBtr0088391) HSP 1 Score: 157.532 bits (397), Expect = 5.281e-39 Identity = 135/483 (27.95%), Postives = 224/483 (46.38%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-------------SNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKF----------------SDNQLNQFTGK-DLTDF----IDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLA--VSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEF-GRQVRLGVRRALPLDTR 1700
++M+GP G GK + + +A+E FF IN L +E + K + A K+ ++ I++ + + ++D + ++S + + PNS RR +E+ + I D R E+ + + M DD + ++ S+ L Q K DL D ID +T+E + S+ PS L + ++ W DIGG ESVK L+ ++ P+++ +F+K GVLF+GPPGC KT+ +A+A E N IS+K ++ + GESE +R IFD+A + P +LF DE+D I +R + R+++++L ++G+ K NV + ATN D +D A++R GR+ I I LPD SRE+IL A +V + + IA+ G SGA++ E R +L +R+A+ + R
Sbjct: 238 ILMYGPPGTGKTLIARAVANET-GAFFFLINGPEIMSKLAGESESNLRKAFEE---AEKNSPAIIFIDEIDAIAPKRDKTHGEVERRIVSQLLTLMDGMKKSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRI---HTKNMKLHDDVDLEQIAAESHGHVGADLASLCSEAALQQIREKMDLIDLEDDKIDAEVLASLAVTMENFRYAMTKSS--PSALRETVVEVPNTTWTDIGGLESVKKELQELVQYPVEHPDKFLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISVKGPELLTMWFGESEANVRDIFDKARSAAPCVLFFDELDSIAKARGGNVGDAGGAADRVINQILTEMDGMGAKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDDKSREAILKANLRKSP----LAKEV--DLTYIAKVTQGFSGADLTEICQRACKLAIRQAIEAEIR 705 HSP 2 Score: 153.295 bits (386), Expect = 1.142e-37 Identity = 89/272 (32.72%), Postives = 144/272 (52.94%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSN--SKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGI----------------VREMKRKAQFHNNNENV--SDSLPTIKYQYFREAI 3263
WTDIGG E+VK L E ++ P++H G++ +G+LF+GPPGC KT+ A+A ECQ +V +++ GESEA R I +A ++P ++F +++D + R N +R++++++T + G+ KK NV I+ TN +D A+LRPGRL ++ I LPD K RE+ILK E +++ IA+ G SGA++ I +R K +A+ N+ ++ D +P I +F EA+
Sbjct: 473 WTDIGGLESVKKELQELVQYPVEHPDKFLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISVKGPELLTMWFGESEANVRDIFDKARSAAPCVLFFDELDSIAKARGGNVGDAGGAADRVINQILTEMDGMGAKK--NVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDDKSREAILKANLRKSPLAKE----VDLTYIAKVTQGFSGADLTEICQRACKLAIRQAIEAEIRREKERAENQNSAMDMDEDDPVPEITSAHFEEAM 738 HSP 3 Score: 127.102 bits (318), Expect = 2.134e-29 Identity = 73/222 (32.88%), Postives = 125/222 (56.31%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
+ DIGG + E +E+P++H G++ P+GIL +GPPG KT+ A+A E F + +IM + GESE+ R +A ++SP++IFI++ID + PKR + V RI+S+L+T + G+ KK+ +++++A TN +++D AL R GR + IG+PD R +L+I ++ H+ + ++ IA G GA++ + E
Sbjct: 200 YDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPPRGILMYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNSPAIIFIDEIDAIAPKR-DKTHGEVERRIVSQLLTLMDGM--KKSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRIHTKNMKL---HDDV-DLEQIAAESHGHVGADLASLCSE 414 HSP 4 Score: 126.331 bits (316), Expect = 3.378e-29 Identity = 80/230 (34.78%), Postives = 117/230 (50.87%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
L + + DIGG +K +E PL++ G + +G+L +GPPG KT+ RA+A ETG I IM GESE LR+ F+ A P+I+FIDEID I P RD V +R++S+LL ++G+ + ++I +AATN + +D A+ R GR I+IG+PD R +L I DDV + IA G GA++ + L
Sbjct: 195 LNAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPPRGILMYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNSPAIIFIDEIDAIAPKRDKTH-GEVERRIVSQLLTLMDGMKKSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRI----HTKNMKLHDDV--DLEQIAAESHGHVGADLASLCSEAAL 417
BLAST of AAA family ATPase vs. Ensembl Fly
Match: TER94 (gene:FBgn0286784 transcript:FBtr0112905) HSP 1 Score: 157.147 bits (396), Expect = 7.740e-39 Identity = 135/483 (27.95%), Postives = 224/483 (46.38%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-------------SNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKF----------------SDNQLNQFTGK-DLTDF----IDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLA--VSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEF-GRQVRLGVRRALPLDTR 1700
++M+GP G GK + + +A+E FF IN L +E + K + A K+ ++ I++ + + ++D + ++S + + PNS RR +E+ + I D R E+ + + M DD + ++ S+ L Q K DL D ID +T+E + S+ PS L + ++ W DIGG ESVK L+ ++ P+++ +F+K GVLF+GPPGC KT+ +A+A E N IS+K ++ + GESE +R IFD+A + P +LF DE+D I +R + R+++++L ++G+ K NV + ATN D +D A++R GR+ I I LPD SRE+IL A +V + + IA+ G SGA++ E R +L +R+A+ + R
Sbjct: 263 ILMYGPPGTGKTLIARAVANET-GAFFFLINGPEIMSKLAGESESNLRKAFEE---AEKNSPAIIFIDEIDAIAPKRDKTHGEVERRIVSQLLTLMDGMKKSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRI---HTKNMKLHDDVDLEQIAAESHGHVGADLASLCSEAALQQIREKMDLIDLEDDKIDAEVLASLAVTMENFRYAMTKSS--PSALRETVVEVPNTTWTDIGGLESVKKELQELVQYPVEHPDKFLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISVKGPELLTMWFGESEANVRDIFDKARSAAPCVLFFDELDSIAKARGGNVGDAGGAADRVINQILTEMDGMGAKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDDKSREAILKANLRKSP----LAKEV--DLTYIAKVTQGFSGADLTEICQRACKLAIRQAIEAEIR 730 HSP 2 Score: 153.68 bits (387), Expect = 9.784e-38 Identity = 89/272 (32.72%), Postives = 144/272 (52.94%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSN--SKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGI----------------VREMKRKAQFHNNNENV--SDSLPTIKYQYFREAI 3263
WTDIGG E+VK L E ++ P++H G++ +G+LF+GPPGC KT+ A+A ECQ +V +++ GESEA R I +A ++P ++F +++D + R N +R++++++T + G+ KK NV I+ TN +D A+LRPGRL ++ I LPD K RE+ILK E +++ IA+ G SGA++ I +R K +A+ N+ ++ D +P I +F EA+
Sbjct: 498 WTDIGGLESVKKELQELVQYPVEHPDKFLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISVKGPELLTMWFGESEANVRDIFDKARSAAPCVLFFDELDSIAKARGGNVGDAGGAADRVINQILTEMDGMGAKK--NVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDDKSREAILKANLRKSPLAKE----VDLTYIAKVTQGFSGADLTEICQRACKLAIRQAIEAEIRREKERAENQNSAMDMDEDDPVPEITSAHFEEAM 763 HSP 3 Score: 127.872 bits (320), Expect = 1.427e-29 Identity = 73/222 (32.88%), Postives = 125/222 (56.31%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
+ DIGG + E +E+P++H G++ P+GIL +GPPG KT+ A+A E F + +IM + GESE+ R +A ++SP++IFI++ID + PKR + V RI+S+L+T + G+ KK+ +++++A TN +++D AL R GR + IG+PD R +L+I ++ H+ + ++ IA G GA++ + E
Sbjct: 225 YDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPPRGILMYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNSPAIIFIDEIDAIAPKR-DKTHGEVERRIVSQLLTLMDGM--KKSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRIHTKNMKL---HDDV-DLEQIAAESHGHVGADLASLCSE 439 HSP 4 Score: 126.716 bits (317), Expect = 2.926e-29 Identity = 80/230 (34.78%), Postives = 117/230 (50.87%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
L + + DIGG +K +E PL++ G + +G+L +GPPG KT+ RA+A ETG I IM GESE LR+ F+ A P+I+FIDEID I P RD V +R++S+LL ++G+ + ++I +AATN + +D A+ R GR I+IG+PD R +L I DDV + IA G GA++ + L
Sbjct: 220 LNAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPPRGILMYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNSPAIIFIDEIDAIAPKRDKTH-GEVERRIVSQLLTLMDGMKKSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRI----HTKNMKLHDDV--DLEQIAAESHGHVGADLASLCSEAAL 442
BLAST of AAA family ATPase vs. Ensembl Fly
Match: TER94 (gene:FBgn0286784 transcript:FBtr0343852) HSP 1 Score: 157.147 bits (396), Expect = 8.485e-39 Identity = 135/483 (27.95%), Postives = 224/483 (46.38%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-------------SNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKF----------------SDNQLNQFTGK-DLTDF----IDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLA--VSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEF-GRQVRLGVRRALPLDTR 1700
++M+GP G GK + + +A+E FF IN L +E + K + A K+ ++ I++ + + ++D + ++S + + PNS RR +E+ + I D R E+ + + M DD + ++ S+ L Q K DL D ID +T+E + S+ PS L + ++ W DIGG ESVK L+ ++ P+++ +F+K GVLF+GPPGC KT+ +A+A E N IS+K ++ + GESE +R IFD+A + P +LF DE+D I +R + R+++++L ++G+ K NV + ATN D +D A++R GR+ I I LPD SRE+IL A +V + + IA+ G SGA++ E R +L +R+A+ + R
Sbjct: 263 ILMYGPPGTGKTLIARAVANET-GAFFFLINGPEIMSKLAGESESNLRKAFEE---AEKNSPAIIFIDEIDAIAPKRDKTHGEVERRIVSQLLTLMDGMKKSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRI---HTKNMKLHDDVDLEQIAAESHGHVGADLASLCSEAALQQIREKMDLIDLEDDKIDAEVLASLAVTMENFRYAMTKSS--PSALRETVVEVPNTTWTDIGGLESVKKELQELVQYPVEHPDKFLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISVKGPELLTMWFGESEANVRDIFDKARSAAPCVLFFDELDSIAKARGGNVGDAGGAADRVINQILTEMDGMGAKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDDKSREAILKANLRKSP----LAKEV--DLTYIAKVTQGFSGADLTEICQRACKLAIRQAIEAEIR 730 HSP 2 Score: 154.451 bits (389), Expect = 5.507e-38 Identity = 89/271 (32.84%), Postives = 144/271 (53.14%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSN--SKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGI----------------VREMKRKAQFHNNN-ENVSDSLPTIKYQYFREAI 3263
WTDIGG E+VK L E ++ P++H G++ +G+LF+GPPGC KT+ A+A ECQ +V +++ GESEA R I +A ++P ++F +++D + R N +R++++++T + G+ KK NV I+ TN +D A+LRPGRL ++ I LPD K RE+ILK E +++ IA+ G SGA++ I +R K +A+ N+ ++ D +P I +F EA+
Sbjct: 498 WTDIGGLESVKKELQELVQYPVEHPDKFLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISVKGPELLTMWFGESEANVRDIFDKARSAAPCVLFFDELDSIAKARGGNVGDAGGAADRVINQILTEMDGMGAKK--NVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDDKSREAILKANLRKSPLAKE----VDLTYIAKVTQGFSGADLTEICQRACKLAIRQAIEAEIRREKERAENQNSAMDDEDDPVPEITSAHFEEAM 762 HSP 3 Score: 127.872 bits (320), Expect = 1.474e-29 Identity = 73/222 (32.88%), Postives = 125/222 (56.31%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
+ DIGG + E +E+P++H G++ P+GIL +GPPG KT+ A+A E F + +IM + GESE+ R +A ++SP++IFI++ID + PKR + V RI+S+L+T + G+ KK+ +++++A TN +++D AL R GR + IG+PD R +L+I ++ H+ + ++ IA G GA++ + E
Sbjct: 225 YDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPPRGILMYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNSPAIIFIDEIDAIAPKR-DKTHGEVERRIVSQLLTLMDGM--KKSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRIHTKNMKL---HDDV-DLEQIAAESHGHVGADLASLCSE 439 HSP 4 Score: 126.716 bits (317), Expect = 3.048e-29 Identity = 80/230 (34.78%), Postives = 117/230 (50.87%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
L + + DIGG +K +E PL++ G + +G+L +GPPG KT+ RA+A ETG I IM GESE LR+ F+ A P+I+FIDEID I P RD V +R++S+LL ++G+ + ++I +AATN + +D A+ R GR I+IG+PD R +L I DDV + IA G GA++ + L
Sbjct: 220 LNAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPPRGILMYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNSPAIIFIDEIDAIAPKRDKTH-GEVERRIVSQLLTLMDGMKKSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRI----HTKNMKLHDDV--DLEQIAAESHGHVGADLASLCSEAAL 442
BLAST of AAA family ATPase vs. Ensembl Fly
Match: CG5776 (gene:FBgn0032450 transcript:FBtr0329925) HSP 1 Score: 147.902 bits (372), Expect = 6.428e-36 Identity = 95/295 (32.20%), Postives = 158/295 (53.56%), Query Frame = 3
Query: 2346 SVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQ---LIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR----SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
S E++E + HG+ D+ + +A ++ ++ +R + +E+ + + W+DIGG ++ + + IE P+ H G++ P+GIL GPPGC KTM ALATE +L ++ ++ VGESE A R + +A + +P+++F ++ID + +R S+S S+V ER+L++L+T L G+E QNV IVA TN +D+ALLRPGR+ I+ +GLP + R ILKI + + ++ + + G SGAEI + E +A
Sbjct: 470 SDEEVEHVASITHGYVGADLANLVYAAMLQAQPNPLQMPHLQAALTRIKPSAMREVLIECPNVQWSDIGGQSELRLAMQQAIEWPLLHADKFQRLGIKPPRGILMFGPPGCSKTMIAKALATESKLNFLSIKGPELFSMWVGESERAVREVFRKARQVAPAIVFFDEIDAIGGERSEGDGSSSGSSVKERVLTQLLTELDGVEAL--QNVTIVAATNRPDMIDKALLRPGRIDRILYVGLPQCEARREILKIKLRAMPISND----VDMEKLVQLTEGYSGAEIQAVCHEAALRA 758 HSP 2 Score: 143.28 bits (360), Expect = 1.972e-34 Identity = 85/235 (36.17%), Postives = 130/235 (55.32%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR----DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEI 1640
PS + + + + W DIGG +++ ++ IE PL + +F +G+L GPPGCSKTM +ALATE+ LN +SIK + +VGESE +R++F +A + P+I+F DEID I R + S V +R++++LL L+G+ NV VAATN D +D A++R GR+ + +GLP +R IL I ++DV + + + G SGAEI
Sbjct: 519 PSAMREVLIECPNVQWSDIGGQSELRLAMQQAIEWPLLHADKFQRLGIKPPRGILMFGPPGCSKTMIAKALATESKLNFLSIKGPELFSMWVGESERAVREVFRKARQVAPAIVFFDEIDAIGGERSEGDGSSSGSSVKERVLTQLLTELDGVEALQNVTIVAATNRPDMIDKALLRPGRIDRILYVGLPQCEARREILKIKLR----AMPISNDV--DMEKLVQLTEGYSGAEI 747 HSP 3 Score: 72.7886 bits (177), Expect = 1.288e-12 Identity = 57/260 (21.92%), Postives = 122/260 (46.92%), Query Frame = 3
Query: 2535 DIGGYETVKTMLIENIEMPI-----KHGLEIPKGILFHGPPGCPKTMFVNALA------TECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALE--SSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETK---KNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHN-NNENVSDSLPTIKYQYFREAI 3263
IGG + ++ E++E + GL + +G+L +G GC K+M + A+ ++ +++ + ++ +GE+E AI +A P+L+ IE + L PK+ + S++ +R+ ++ L + + K ++AT++++ + ++ R GRL V +G P + R I++ + Q E + ++++I G GA++ +V +AQ + ++ +L IK RE +
Sbjct: 273 QIGGLDRQLQLVEESMEYALGFRTLPAGLRVSRGLLLYGATGCGKSMVLEAMCAVAEERSQGHVQLIRINSGEVYSKFLGETEQKLGAIFERAYNHYPHPTLLLIEDVHNLCPKQEN---SDLVKRVSLAFLSLLDQLSSPSQLKGSKTFVLATSSQIDTLHPSIRRAGRLDNEVELGAPSSQARLEIVRCLIKSVEHQLSDEEVEHVASITH---GYVGADLANLVYAAMLQAQPNPLQMPHLQAALTRIKPSAMREVL 526 HSP 4 Score: 61.6178 bits (148), Expect = 3.659e-9 Identity = 44/165 (26.67%), Postives = 84/165 (50.91%), Query Frame = 3
Query: 1077 LKNGKEFVKGVLFHGPPGCSKTMFVRALA------TETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAV--ALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINE----KPNVIFVAATNFK-DKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
L G +G+L +G GC K+M + A+ ++ + LI I + ++GE+E L IF+RA P++L I+++ + P +++ S + +R+ L L+ ++ K + FV AT+ + D L ++ R+GR+ +E+G P +R I+
Sbjct: 297 LPAGLRVSRGLLLYGATGCGKSMVLEAMCAVAEERSQGHVQLIRINSGEVYSKFLGETEQKLGAIFERAYNHYPHPTLLLIEDVHNLCPKQEN---SDLVKRVSLAFLSLLDQLSSPSQLKGSKTFVLATSSQIDTLHPSIRRAGRLDNEVELGAPSSQARLEIV 458
BLAST of AAA family ATPase vs. Ensembl Zebrafish
Match: vcp (valosin containing protein [Source:NCBI gene;Acc:327197]) HSP 1 Score: 154.066 bits (388), Expect = 8.623e-38 Identity = 131/476 (27.52%), Postives = 224/476 (47.06%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-SNLNEEFH------------LIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF-----IDNVYRKFKEITLE----------RLKACRKDSNWI-----PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR--DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEF-GRQVRLGVRRA 1682
++++GP G GK + + +A+E FF IN L +E + K + A K+ ++ +++ + E+ H ++D L + + + + PNS RR +EV + I D R E+ + + M DD + ++ + N+ + G DL + + +K I LE L D W PS L + ++ + W DIGG + VK L+ ++ P+++ +F+K GVLF+GPPGC KT+ +A+A E N ISIK ++ + GESE +R+IFD+A P +LF DE+D I +R + + R+++++L ++G++ K NV + ATN D +D A++R GR+ I I LPD+ SR +IL + DV +F +A+ +G SGA++ E R +L +R +
Sbjct: 241 ILLYGPPGTGKTLIARAVANET-GAFFFLINGPEIMSKLAGESESNLRKAFEE---AEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQI---HTKNMKLADDVDLEQVA-NETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPNITWEDIGGLDDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNVGDGGGAADRVINQILTEMDGMSSKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRIAILKANLRKSP----ISKDVDLDF--LAKMTNGFSGADLTEICQRACKLAIRES 702 HSP 2 Score: 140.584 bits (353), Expect = 1.903e-33 Identity = 96/325 (29.54%), Postives = 161/325 (49.54%), Query Frame = 3
Query: 2223 DLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVR 3170
DL+ V ++ G V + +E L+ + K+ I E I+ V N T D RW L S+++ L E E+ N + W DIGG + VK L E ++ P++H G+ KG+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R I +A +++P ++F +++D + R + +R++++++T + G+ +KK NV I+ TN +D A+LRPGRL ++ I LPD+K R +ILK + ++ +A+ G SGA++ I +
Sbjct: 395 DLEQVANETHGHVGADLAALC---SEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWAL-------SQSNPSALRETVVEVPNIT-----------WEDIGGLDDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNVGDGGGAADRVINQILTEMDGMSSKK--NVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRIAILKANLRKSPISKD----VDLDFLAKMTNGFSGADLTEICQ 692 HSP 3 Score: 130.568 bits (327), Expect = 2.448e-30 Identity = 81/239 (33.89%), Postives = 124/239 (51.88%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRALPL 1691
L + + DIGG +K +E PL++ G + +G+L +GPPG KT+ RA+A ETG I IM GESE LR+ F+ A P+I+FIDE+D I P R+ V +R++S+LL ++G+ ++ +VI +AATN + +D A+ R GR ++IG+PD R IL I ADDV + +A G GA++ + L +R+ + L
Sbjct: 198 LNEVGYDDIGGVRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTH-GEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQI----HTKNMKLADDV--DLEQVANETHGHVGADLAALCSEAALQAIRKKMDL 429 HSP 4 Score: 127.487 bits (319), Expect = 2.416e-29 Identity = 72/227 (31.72%), Postives = 124/227 (54.63%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
+ DIGG + E +E+P++H G++ P+GIL +GPPG KT+ A+A E F + +IM + GESE+ R +A +++P++IFI+++D + PKR + V RI+S+L+T + G+ K+ +V+++A TN +++D AL R GR V IG+PD R IL+I ++ + ++ +A G GA++ + E +A
Sbjct: 203 YDDIGGVRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREK-THGEVERRIVSQLLTLMDGL--KQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADD----VDLEQVANETHGHVGADLAALCSEAALQA 422
BLAST of AAA family ATPase vs. Ensembl Zebrafish
Match: vcp (valosin containing protein [Source:NCBI gene;Acc:327197]) HSP 1 Score: 154.066 bits (388), Expect = 9.499e-38 Identity = 131/476 (27.52%), Postives = 224/476 (47.06%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-SNLNEEFH------------LIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF-----IDNVYRKFKEITLE----------RLKACRKDSNWI-----PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR--DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEF-GRQVRLGVRRA 1682
++++GP G GK + + +A+E FF IN L +E + K + A K+ ++ +++ + E+ H ++D L + + + + PNS RR +EV + I D R E+ + + M DD + ++ + N+ + G DL + + +K I LE L D W PS L + ++ + W DIGG + VK L+ ++ P+++ +F+K GVLF+GPPGC KT+ +A+A E N ISIK ++ + GESE +R+IFD+A P +LF DE+D I +R + + R+++++L ++G++ K NV + ATN D +D A++R GR+ I I LPD+ SR +IL + DV +F +A+ +G SGA++ E R +L +R +
Sbjct: 241 ILLYGPPGTGKTLIARAVANET-GAFFFLINGPEIMSKLAGESESNLRKAFEE---AEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQI---HTKNMKLADDVDLEQVA-NETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPNITWEDIGGLDDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNVGDGGGAADRVINQILTEMDGMSSKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRIAILKANLRKSP----ISKDVDLDF--LAKMTNGFSGADLTEICQRACKLAIRES 702 HSP 2 Score: 140.198 bits (352), Expect = 2.005e-33 Identity = 96/325 (29.54%), Postives = 161/325 (49.54%), Query Frame = 3
Query: 2223 DLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVR 3170
DL+ V ++ G V + +E L+ + K+ I E I+ V N T D RW L S+++ L E E+ N + W DIGG + VK L E ++ P++H G+ KG+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R I +A +++P ++F +++D + R + +R++++++T + G+ +KK NV I+ TN +D A+LRPGRL ++ I LPD+K R +ILK + ++ +A+ G SGA++ I +
Sbjct: 395 DLEQVANETHGHVGADLAALC---SEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWAL-------SQSNPSALRETVVEVPNIT-----------WEDIGGLDDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNVGDGGGAADRVINQILTEMDGMSSKK--NVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRIAILKANLRKSPISKD----VDLDFLAKMTNGFSGADLTEICQ 692 HSP 3 Score: 130.568 bits (327), Expect = 2.670e-30 Identity = 81/239 (33.89%), Postives = 124/239 (51.88%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRALPL 1691
L + + DIGG +K +E PL++ G + +G+L +GPPG KT+ RA+A ETG I IM GESE LR+ F+ A P+I+FIDE+D I P R+ V +R++S+LL ++G+ ++ +VI +AATN + +D A+ R GR ++IG+PD R IL I ADDV + +A G GA++ + L +R+ + L
Sbjct: 198 LNEVGYDDIGGVRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTH-GEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQI----HTKNMKLADDV--DLEQVANETHGHVGADLAALCSEAALQAIRKKMDL 429 HSP 4 Score: 127.102 bits (318), Expect = 2.634e-29 Identity = 72/227 (31.72%), Postives = 124/227 (54.63%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
+ DIGG + E +E+P++H G++ P+GIL +GPPG KT+ A+A E F + +IM + GESE+ R +A +++P++IFI+++D + PKR + V RI+S+L+T + G+ K+ +V+++A TN +++D AL R GR V IG+PD R IL+I ++ + ++ +A G GA++ + E +A
Sbjct: 203 YDDIGGVRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKR-EKTHGEVERRIVSQLLTLMDGL--KQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADD----VDLEQVANETHGHVGADLAALCSEAALQA 422
BLAST of AAA family ATPase vs. Ensembl Zebrafish
Match: zgc:136908 (zgc:136908 [Source:ZFIN;Acc:ZDB-GENE-060312-22]) HSP 1 Score: 140.969 bits (354), Expect = 1.225e-33 Identity = 130/482 (26.97%), Postives = 216/482 (44.81%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-SNLNEEFH------------LIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF-----IDNVYRKFKEITLE----------RLKACRKDSNWI-----PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMI--FPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENF-SAIAERLSGKSGAEIVEFGRQVRLGVRRALPLDTR 1700
++++GP G GK + + +A+E FF IN L +E + K + A K+ ++ +++ + E+ H ++D L + + + + PNS RR +E+ + I D R E+ + + M +D + ++ S + + G DL + + +K I LE L D W PS L + ++ ++W DIGG + VK L+ ++ P++ +F+K GVLF+GPPGC KT+ +A+A E N +SIK ++ + GESE +R +FD+A P ILF DE+D I + R+++++L ++G+ K NV + ATN D +D A++R GR+ I I LPD SR +IL A DV + S I E SG EI + R +L +R A+ + R
Sbjct: 243 ILLYGPPGTGKTLVARAVANET-GAFFFLINGPEIMSKLAGESESNLRKAFEE---AEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVVVMAATNRPNSVDAALRRFGRFDREIDIGIPDSTGRLEILQI---HTKNMKLSEDVDLEQIS-AETHGHVGADLAALCSEAALQAIRKKMTLIDLEDDSIDADLLNSLAVTMDDFKWALSQSNPSALRETVVEVPHVNWEDIGGLDEVKRELQELVQYPVEYPDKFLKFGMTPSRGVLFYGPPGCGKTLLAKAIANECQANFVSIKGPELLTMWFGESEANVRDVFDKARQAAPCILFFDELDSIAKARGGGAGDAGGAADRVINQILTEMDGMTNKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDMPSRTAILRANLRKSP----VAKDVDLMYLSKITEGFSGADLTEICQ--RACKLAIREAIEAEIR 710 HSP 2 Score: 128.642 bits (322), Expect = 1.030e-29 Identity = 115/479 (24.01%), Postives = 213/479 (44.47%), Query Frame = 3
Query: 1992 EHLESTMQQTIFIRLSDKLATKEISEVIEVINHGNSKRK---DMVVVVDGMRKMSDDFCEAVQLTLINEAPNVKVMMIFEDLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVE-DIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELD----------------HQSQP------------LHWTDIGGYETVKTMLIENIEMPI-------KHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLL--IPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEI--------------VGIVREMKRKAQFHNNNENVSDSLPTIKYQYFREAI 3263
E E IFI D +A K HG +R+ ++ ++DG+++ + V + N +V + + G ++++ + +P D+ L+ + N+K S + D+E++ HG D+ A+++ R M + ++E+ S + D QS P ++W DIGG + VK L E ++ P+ K G+ +G+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R + +A +++P ++F +++D + + +R++++++T + G+ KK NV I+ TN +D A+LRPGRL ++ I LPD R +IL+ + +++ +S I E +G EI I E +R+A+ ++ D +P I+ +F EA+
Sbjct: 293 EEAEKNAPAIIFIDELDAIAPKREK------THGEVERRIVSQLLTLMDGLKQRA----HVVVMAATNRPNSVDAAL----------RRFGRFDREIDIGIP-DSTGRLEILQIHTKNMKLSEDVDLEQISAETHGHVGADLAALCSEAALQAIRKKMTLI-----DLEDDSIDADLLNSLAVTMDDFKWALSQSNPSALRETVVEVPHVNWEDIGGLDEVKRELQELVQYPVEYPDKFLKFGMTPSRGVLFYGPPGCGKTLLAKAIANECQANFVSIKGPELLTMWFGESEANVRDVFDKARQAAPCILFFDELDSIAKARGGGAGDAGGAADRVINQILTEMDGMTNKK--NVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDMPSRTAILRANLRKSPVAKDVDLMY-LSKITEGFSGADLTEICQRACKLAIREAIEAEIRAERQRQARKETAMDDDYDPVPEIRKDHFEEAM 742 HSP 3 Score: 127.487 bits (319), Expect = 2.473e-29 Identity = 80/239 (33.47%), Postives = 125/239 (52.30%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRALPL 1691
L + + DIGG +K +E PL++ G + +G+L +GPPG KT+ RA+A ETG I IM GESE LR+ F+ A P+I+FIDE+D I P R+ V +R++S+LL ++G+ ++ +V+ +AATN + +DAA+ R GR I+IG+PD R IL I ++DV + I+ G GA++ + L +R+ + L
Sbjct: 200 LNDIGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLVARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTH-GEVERRIVSQLLTLMDGLKQRAHVVVMAATNRPNSVDAALRRFGRFDREIDIGIPDSTGRLEILQI----HTKNMKLSEDV--DLEQISAETHGHVGADLAALCSEAALQAIRKKMTL 431 HSP 4 Score: 125.561 bits (314), Expect = 8.100e-29 Identity = 72/229 (31.44%), Postives = 125/229 (54.59%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
+ + DIGG + E +E+P++H G++ P+GIL +GPPG KT+ A+A E F + +IM + GESE+ R +A +++P++IFI+++D + PKR + V RI+S+L+T + G+ K+ +V+++A TN ++VD AL R GR + IG+PD R IL+I ++ + ++ I+ G GA++ + E +A
Sbjct: 203 IGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLVARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKR-EKTHGEVERRIVSQLLTLMDGL--KQRAHVVVMAATNRPNSVDAALRRFGRFDREIDIGIPDSTGRLEILQIHTKNMKLSED----VDLEQISAETHGHVGADLAALCSEAALQA 424
BLAST of AAA family ATPase vs. Ensembl Zebrafish
Match: nvl (nuclear VCP like [Source:ZFIN;Acc:ZDB-GENE-040426-2871]) HSP 1 Score: 138.272 bits (347), Expect = 9.291e-33 Identity = 116/445 (26.07%), Postives = 200/445 (44.94%), Query Frame = 3
Query: 417 VMHGPRGCGKKSLMKRLADE-----MPNKSFFWINCLTWFNEKDYLKIIYQTLIANKSIQMVLCCYFIN----------SSNLNEEFHLI---------IDNLVENSQIDIKILLEIPNSDSVDFRRR----KEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF-----IDNVYRKFKEITLE-------------------------RLKA-CRKDSNWI-------PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
++HGP GCGK L + +A E + + ++ ++ +E+ ++ Q +I C FI+ +++ + E ++ +++++E +Q+ + P+S RR +E+ + I D+ +R ++ K L + D+FD + + + G DL ++ V R E T E +L A C S++ PS A + + W D+G + V+ L I P++N ++F G+L GPPGC KT+ +A+A E+GLN IS+K ++ YVGESE +RQ+F R P ++F DEID + P R S S R++++LL ++G+ + V +AATN D +D AV+R GR+ + +GLP R IL
Sbjct: 264 LLHGPPGCGKTLLAQAVAGETALPLLKISAPELVSGVSGESEQKLRELFEQ------AISSAPCILFIDEIDAITPKRETASKDMERRIVAQLLTCMDDLNSMLEPAQVLVIGATNRPDSLDPALRRAGRFDREICLGIPDEGARMKILKTLCRKIRLPDDFDFRHLARLTPG----YVGADLMALCREAAMNAVNRILLEPTTEDQTTPTLALARLLDLLKSSVSLSEDQLAALCVLMSDFSSSLVRVQPSAKREGFATVPDVTWADVGALQDVREELHMAIMAPIQNPEQFKALGLSAPAGLLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRGRNSAPCVIFFDEIDALCPRRSEHE-SGASVRVVNQLLTEMDGMENRRQVFIMAATNRPDIIDPAVLRPGRLDKTLYVGLPPAADRHVIL 697 HSP 2 Score: 127.872 bits (320), Expect = 1.471e-29 Identity = 78/224 (34.82%), Postives = 122/224 (54.46%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILK-IGFSDLNFQTEHEI-ISNISTIAESLTGLSGAEIVGIVRE 3173
W D+G + V+ L I PI++ GL P G+L GPPGC KT+ A+A E L +V +++ VGESE A R + + S+P +IF ++ID L P+RS + +S R++++L+T + G+E ++ V I+A TN +D A+LRPGRL + +GLP DR IL I Q + ++ + I+ A T +GA++ +VRE
Sbjct: 519 WADVGALQDVREELHMAIMAPIQNPEQFKALGLSAPAGLLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRGRNSAPCVIFFDEIDALCPRRSEH-ESGASVRVVNQLLTEMDGMENRR--QVFIMAATNRPDIIDPAVLRPGRLDKTLYVGLPPAADRHVILNTITKGGTKPQLDSDVSLEEIAHDARCET-FTGADLSALVRE 738 HSP 3 Score: 123.635 bits (309), Expect = 3.569e-28 Identity = 70/192 (36.46%), Postives = 108/192 (56.25%), Query Frame = 3
Query: 1101 KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGIN---EKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
+G L HGPPGC KT+ +A+A ET L L+ I ++ G GESE LR++F++A++ P ILFIDEID I P R+ A + +R++++LL ++ +N E V+ + ATN D LD A+ R+GR I +G+PD+ +R IL DD +F +A G GA+++ R+ +
Sbjct: 261 RGFLLHGPPGCGKTLLAQAVAGETALPLLKISAPELVSGVSGESEQKLRELFEQAISSAPCILFIDEIDAITPKRE-TASKDMERRIVAQLLTCMDDLNSMLEPAQVLVIGATNRPDSLDPALRRAGRFDREICLGIPDEGARMKILKTLCRK----IRLPDDF--DFRHLARLTPGYVGADLMALCREAAM 445 HSP 4 Score: 117.472 bits (293), Expect = 2.691e-26 Identity = 71/233 (30.47%), Postives = 124/233 (53.22%), Query Frame = 3
Query: 2496 KELDHQSQPLHWTDIGG----YETVKTMLIE--NIEMPIKHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETK-KNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
K + Q + + D GG E V +LI + E+ + G+ P+G L HGPPGC KT+ A+A E L + + +++ G+ GESE R + QA+ S+P ++FI++ID + PKR + SK ++ RI+++L+T + + + + V+++ TN ++D AL R GR + +G+PD+ R ILK + + + +A G GA+++ + RE
Sbjct: 215 KGFELQFSSVKFEDFGGSDETLEEVCKLLIHMRHPEVYQRLGVVPPRGFLLHGPPGCGKTLLAQAVAGETALPLLKISAPELVSGVSGESEQKLRELFEQAISSAPCILFIDEIDAITPKRETASK-DMERRIVAQLLTCMDDLNSMLEPAQVLVIGATNRPDSLDPALRRAGRFDREICLGIPDEGARMKILKTLCRKIRLPDDF----DFRHLARLTPGYVGADLMALCRE 442
BLAST of AAA family ATPase vs. Ensembl Zebrafish
Match: katnal1 (katanin p60 subunit A-like 1 [Source:NCBI gene;Acc:492790]) HSP 1 Score: 125.946 bits (315), Expect = 8.253e-30 Identity = 80/239 (33.47%), Postives = 126/239 (52.72%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIK-----HGLEIP-KGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKK-----NQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE-----MKRKAQ 3191
+HW DI E K +L E + +P+ G+ P KG+L GPPG KTM A+ATEC FNV S + GESE R + A +P+ IFI++ID + +R ++ + R+ SEL+ + G+ + ++ VM++A TN ++DEAL R RL + I LP K R +LKI +++ ++ +++ IAE + G SGA+I + R+ M+R+ Q
Sbjct: 203 IHWDDIADLEDAKKLLREAVVLPMWMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSICGRRGTSDEHEASRRVKSELLVQMDGVGGAQESEDPSKMVMVLAATNFPWDIDEALRR--RLEKRIYIPLPTAKGRAELLKINLREVDVASD----VDLTVIAEKIEGYSGADITNVCRDASMMAMRRRIQ 435 HSP 2 Score: 120.939 bits (302), Expect = 3.399e-28 Identity = 85/240 (35.42%), Postives = 125/240 (52.08%), Query Frame = 3
Query: 1008 LHWVDIGGYESVKVMLKNEIEEPL------KNGKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGI-----NEKPN--VIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVR-LGVRRAL 1685
+HW DI E K +L+ + P+ K + KGVL GPPG KTM +A+ATE G ++ ST+ Y GESE L+R +F+ A P+ +FIDEID I R S+R+ SELL+ ++G+ +E P+ V+ +AATNF +D A+ R R+ I I LP R +L I + + A DV + + IAE++ G SGA+I R + +RR +
Sbjct: 203 IHWDDIADLEDAKKLLREAVVLPMWMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSICGRRGTSDEHEASRRVKSELLVQMDGVGGAQESEDPSKMVMVLAATNFPWDIDEALRR--RLEKRIYIPLPTAKGRAELLKINLREVD----VASDV--DLTVIAEKIEGYSGADITNVCRDASMMAMRRRI 434
BLAST of AAA family ATPase vs. Ensembl Xenopus
Match: SPATA5 (spermatogenesis associated 5 [Source:NCBI gene;Acc:100494828]) HSP 1 Score: 166.007 bits (419), Expect = 2.490e-41 Identity = 95/240 (39.58%), Postives = 136/240 (56.67%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
PS + D+ + W DIGG E+VK+ LK +E PL + F+ KGVL +GPPGCSKTM +ALA E+GLN +++K +M YVGESE +R+IF +A A+ PSILF DEID + R A S V+ R++++LL ++GI + +V+ +AATN D +D A+MR GR+ I + LPD +R I + F + + E+ SGAEI R+ L
Sbjct: 556 PSAMREVAIDVPNVSWSDIGGLENVKLKLKQAVEWPLMHPDSFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAVKGPELMNKYVGESERAVREIFHKARAVAPSILFFDEIDALAIERGSSAGS-VADRVLAQLLTEMDGIEQLKDVVILAATNRPDLIDKALMRPGRIDRIIYVPLPDAATRREIFKLRFHS------MPISTEICLEKLVEQTEKYSGAEITAVCREAAL 788 HSP 2 Score: 162.925 bits (411), Expect = 2.102e-40 Identity = 102/301 (33.89%), Postives = 155/301 (51.50%), Query Frame = 3
Query: 2352 EDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQL-------------------IEFSREIENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
ED+ +L HG+ D+ K A + R R L ++ + E+ ++ +E+ + W+DIGG E VK L + +E P+ H G++ PKG+L +GPPGC KTM ALA E L V ++M VGESE A R I +A +PS++F ++ID L +R S++ S V +R+L++L+T + GIE K +V+I+A TN +D+AL+RPGR+ I+ + LPD R I K+ F + TE + + E SGAEI + RE
Sbjct: 492 EDLAQLADRTHGYVGADLAALCKEAGMNALRRTHRVLSRPSDREMAGSVVITLNDFLQATNEVRPSAMREVAIDVPNVSWSDIGGLENVKLKLKQAVEWPLMHPDSFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAVKGPELMNKYVGESERAVREIFHKARAVAPSILFFDEIDALAIERGSSAGS-VADRVLAQLLTEMDGIEQLK--DVVILAATNRPDLIDKALMRPGRIDRIIYVPLPDAATRREIFKLRFHSMPISTE----ICLEKLVEQTEKYSGAEITAVCRE 785 HSP 3 Score: 110.538 bits (275), Expect = 5.953e-24 Identity = 67/245 (27.35%), Postives = 128/245 (52.24%), Query Frame = 3
Query: 2463 IEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIK-------HGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQ-NVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
I + + T+KE D + + + IGG + + E IE+P+K +G+ P+G+L +GPPG KT+ A+A E + + +I+ Q PS+IFI+++D L PKR +++ V +R+++ L+T + GI ++++Q ++++ TN H++D AL RPGR + IG+P+ + R IL+ + + + E +++ +A+ G GA++ + +E
Sbjct: 288 ITYKEQKTTTAKETDELKRKVTYNMIGGLKAELKEIRETIELPLKQPELFRNYGIPPPRGVLLYGPPGTGKTLIARAIANEVGAHVTVINGPEIVSN-------------EQQCACCPSIIFIDELDALCPKRE-GAQNEVEKRVVASLLTLMDGIGSEESQGQLLVLGATNRPHSLDPALRRPGRFDKEIEIGVPNAQGRLDILQKVLKKVPHRLKEE---DLAQLADRTHGYVGADLAALCKE 515 HSP 4 Score: 93.5893 bits (231), Expect = 1.152e-18 Identity = 67/229 (29.26%), Postives = 109/229 (47.60%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGIN---EKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRR 1679
IGG ++ ++ IE PLK + F +GVL +GPPG KT+ RA+A E G ++ I I+ ++ A PSI+FIDE+D + P R+ A + V +R+++ LL ++GI + ++ + ATN LD A+ R GR IEIG+P+ R IL +E+ + +A+R G GA++ ++ + R
Sbjct: 313 IGGLKAELKEIRETIELPLKQPELFRNYGIPPPRGVLLYGPPGTGKTLIARAIANEVGAHVTVINGPEIVSN-------------EQQCACCPSIIFIDELDALCPKREG-AQNEVEKRVVASLLTLMDGIGSEESQGQLLVLGATNRPHSLDPALRRPGRFDKEIEIGVPNAQGRLDILQKVLKKVPHRL-----KEEDLAQLADRTHGYVGADLAALCKEAGMNALR 522
BLAST of AAA family ATPase vs. Ensembl Xenopus
Match: SPATA5 (spermatogenesis associated 5 [Source:NCBI gene;Acc:100494828]) HSP 1 Score: 165.622 bits (418), Expect = 2.965e-41 Identity = 95/240 (39.58%), Postives = 136/240 (56.67%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
PS + D+ + W DIGG E+VK+ LK +E PL + F+ KGVL +GPPGCSKTM +ALA E+GLN +++K +M YVGESE +R+IF +A A+ PSILF DEID + R A S V+ R++++LL ++GI + +V+ +AATN D +D A+MR GR+ I + LPD +R I + F + + E+ SGAEI R+ L
Sbjct: 546 PSAMREVAIDVPNVSWSDIGGLENVKLKLKQAVEWPLMHPDSFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAVKGPELMNKYVGESERAVREIFHKARAVAPSILFFDEIDALAIERGSSAGS-VADRVLAQLLTEMDGIEQLKDVVILAATNRPDLIDKALMRPGRIDRIIYVPLPDAATRREIFKLRFHS------MPISTEICLEKLVEQTEKYSGAEITAVCREAAL 778 HSP 2 Score: 162.925 bits (411), Expect = 2.257e-40 Identity = 102/301 (33.89%), Postives = 155/301 (51.50%), Query Frame = 3
Query: 2352 EDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQL-------------------IEFSREIENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
ED+ +L HG+ D+ K A + R R L ++ + E+ ++ +E+ + W+DIGG E VK L + +E P+ H G++ PKG+L +GPPGC KTM ALA E L V ++M VGESE A R I +A +PS++F ++ID L +R S++ S V +R+L++L+T + GIE K +V+I+A TN +D+AL+RPGR+ I+ + LPD R I K+ F + TE + + E SGAEI + RE
Sbjct: 482 EDLAQLADRTHGYVGADLAALCKEAGMNALRRTHRVLSRPSDREMAGSVVITLNDFLQATNEVRPSAMREVAIDVPNVSWSDIGGLENVKLKLKQAVEWPLMHPDSFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAVKGPELMNKYVGESERAVREIFHKARAVAPSILFFDEIDALAIERGSSAGS-VADRVLAQLLTEMDGIEQLK--DVVILAATNRPDLIDKALMRPGRIDRIIYVPLPDAATRREIFKLRFHSMPISTE----ICLEKLVEQTEKYSGAEITAVCRE 775 HSP 3 Score: 110.538 bits (275), Expect = 6.837e-24 Identity = 67/245 (27.35%), Postives = 128/245 (52.24%), Query Frame = 3
Query: 2463 IEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIK-------HGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQ-NVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
I + + T+KE D + + + IGG + + E IE+P+K +G+ P+G+L +GPPG KT+ A+A E + + +I+ Q PS+IFI+++D L PKR +++ V +R+++ L+T + GI ++++Q ++++ TN H++D AL RPGR + IG+P+ + R IL+ + + + E +++ +A+ G GA++ + +E
Sbjct: 278 ITYKEQKTTTAKETDELKRKVTYNMIGGLKAELKEIRETIELPLKQPELFRNYGIPPPRGVLLYGPPGTGKTLIARAIANEVGAHVTVINGPEIVSN-------------EQQCACCPSIIFIDELDALCPKRE-GAQNEVEKRVVASLLTLMDGIGSEESQGQLLVLGATNRPHSLDPALRRPGRFDKEIEIGVPNAQGRLDILQKVLKKVPHRLKEE---DLAQLADRTHGYVGADLAALCKE 505 HSP 4 Score: 93.2041 bits (230), Expect = 1.241e-18 Identity = 67/229 (29.26%), Postives = 110/229 (48.03%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPN---VIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRR 1679
IGG ++ ++ IE PLK + F +GVL +GPPG KT+ RA+A E G ++ I I+ ++ A PSI+FIDE+D + P R+ A + V +R+++ LL ++GI + + ++ + ATN LD A+ R GR IEIG+P+ R IL +E+ + +A+R G GA++ ++ + R
Sbjct: 303 IGGLKAELKEIRETIELPLKQPELFRNYGIPPPRGVLLYGPPGTGKTLIARAIANEVGAHVTVINGPEIVSN-------------EQQCACCPSIIFIDELDALCPKREG-AQNEVEKRVVASLLTLMDGIGSEESQGQLLVLGATNRPHSLDPALRRPGRFDKEIEIGVPNAQGRLDILQKVLKKVPHRL-----KEEDLAQLADRTHGYVGADLAALCKEAGMNALR 512
BLAST of AAA family ATPase vs. Ensembl Xenopus
Match: SPATA5 (spermatogenesis associated 5 [Source:NCBI gene;Acc:100494828]) HSP 1 Score: 166.007 bits (419), Expect = 2.979e-41 Identity = 95/240 (39.58%), Postives = 136/240 (56.67%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
PS + D+ + W DIGG E+VK+ LK +E PL + F+ KGVL +GPPGCSKTM +ALA E+GLN +++K +M YVGESE +R+IF +A A+ PSILF DEID + R A S V+ R++++LL ++GI + +V+ +AATN D +D A+MR GR+ I + LPD +R I + F + + E+ SGAEI R+ L
Sbjct: 592 PSAMREVAIDVPNVSWSDIGGLENVKLKLKQAVEWPLMHPDSFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAVKGPELMNKYVGESERAVREIFHKARAVAPSILFFDEIDALAIERGSSAGS-VADRVLAQLLTEMDGIEQLKDVVILAATNRPDLIDKALMRPGRIDRIIYVPLPDAATRREIFKLRFHS------MPISTEICLEKLVEQTEKYSGAEITAVCREAAL 824 HSP 2 Score: 163.31 bits (412), Expect = 2.090e-40 Identity = 102/301 (33.89%), Postives = 155/301 (51.50%), Query Frame = 3
Query: 2352 EDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQL-------------------IEFSREIENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
ED+ +L HG+ D+ K A + R R L ++ + E+ ++ +E+ + W+DIGG E VK L + +E P+ H G++ PKG+L +GPPGC KTM ALA E L V ++M VGESE A R I +A +PS++F ++ID L +R S++ S V +R+L++L+T + GIE K +V+I+A TN +D+AL+RPGR+ I+ + LPD R I K+ F + TE + + E SGAEI + RE
Sbjct: 528 EDLAQLADRTHGYVGADLAALCKEAGMNALRRTHRVLSRPSDREMAGSVVITLNDFLQATNEVRPSAMREVAIDVPNVSWSDIGGLENVKLKLKQAVEWPLMHPDSFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAVKGPELMNKYVGESERAVREIFHKARAVAPSILFFDEIDALAIERGSSAGS-VADRVLAQLLTEMDGIEQLK--DVVILAATNRPDLIDKALMRPGRIDRIIYVPLPDAATRREIFKLRFHSMPISTE----ICLEKLVEQTEKYSGAEITAVCRE 821 HSP 3 Score: 132.88 bits (333), Expect = 7.069e-31 Identity = 74/245 (30.20%), Postives = 137/245 (55.92%), Query Frame = 3
Query: 2463 IEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIK-------HGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQ-NVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
I + + T+KE D + + + IGG + + E IE+P+K +G+ P+G+L +GPPG KT+ A+A E + + +I+ GESEA R I + A + PS+IFI+++D L PKR +++ V +R+++ L+T + GI ++++Q ++++ TN H++D AL RPGR + IG+P+ + R IL+ + + + E +++ +A+ G GA++ + +E
Sbjct: 311 ITYKEQKTTTAKETDELKRKVTYNMIGGLKAELKEIRETIELPLKQPELFRNYGIPPPRGVLLYGPPGTGKTLIARAIANEVGAHVTVINGPEIVSKFYGESEARLRQIFADASQCCPSIIFIDELDALCPKR-EGAQNEVEKRVVASLLTLMDGIGSEESQGQLLVLGATNRPHSLDPALRRPGRFDKEIEIGVPNAQGRLDILQKVLKKVPHRLKEE---DLAQLADRTHGYVGADLAALCKE 551 HSP 4 Score: 117.472 bits (293), Expect = 4.788e-26 Identity = 76/229 (33.19%), Postives = 117/229 (51.09%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGIN---EKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRR 1679
IGG ++ ++ IE PLK + F +GVL +GPPG KT+ RA+A E G ++ I I+ + GESE LRQIF A PSI+FIDE+D + P R+ A + V +R+++ LL ++GI + ++ + ATN LD A+ R GR IEIG+P+ R IL +E+ + +A+R G GA++ ++ + R
Sbjct: 336 IGGLKAELKEIRETIELPLKQPELFRNYGIPPPRGVLLYGPPGTGKTLIARAIANEVGAHVTVINGPEIVSKFYGESEARLRQIFADASQCCPSIIFIDELDALCPKREG-AQNEVEKRVVASLLTLMDGIGSEESQGQLLVLGATNRPHSLDPALRRPGRFDKEIEIGVPNAQGRLDILQKVLKKVPHRL-----KEEDLAQLADRTHGYVGADLAALCKEAGMNALR 558
BLAST of AAA family ATPase vs. Ensembl Xenopus
Match: SPATA5 (spermatogenesis associated 5 [Source:NCBI gene;Acc:100494828]) HSP 1 Score: 165.622 bits (418), Expect = 2.981e-41 Identity = 95/240 (39.58%), Postives = 136/240 (56.67%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
PS + D+ + W DIGG E+VK+ LK +E PL + F+ KGVL +GPPGCSKTM +ALA E+GLN +++K +M YVGESE +R+IF +A A+ PSILF DEID + R A S V+ R++++LL ++GI + +V+ +AATN D +D A+MR GR+ I + LPD +R I + F + + E+ SGAEI R+ L
Sbjct: 555 PSAMREVAIDVPNVSWSDIGGLENVKLKLKQAVEWPLMHPDSFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAVKGPELMNKYVGESERAVREIFHKARAVAPSILFFDEIDALAIERGSSAGS-VADRVLAQLLTEMDGIEQLKDVVILAATNRPDLIDKALMRPGRIDRIIYVPLPDAATRREIFKLRFHS------MPISTEICLEKLVEQTEKYSGAEITAVCREAAL 787 HSP 2 Score: 162.925 bits (411), Expect = 2.110e-40 Identity = 102/301 (33.89%), Postives = 155/301 (51.50%), Query Frame = 3
Query: 2352 EDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQL-------------------IEFSREIENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
ED+ +L HG+ D+ K A + R R L ++ + E+ ++ +E+ + W+DIGG E VK L + +E P+ H G++ PKG+L +GPPGC KTM ALA E L V ++M VGESE A R I +A +PS++F ++ID L +R S++ S V +R+L++L+T + GIE K +V+I+A TN +D+AL+RPGR+ I+ + LPD R I K+ F + TE + + E SGAEI + RE
Sbjct: 491 EDLAQLADRTHGYVGADLAALCKEAGMNALRRTHRVLSRPSDREMAGSVVITLNDFLQATNEVRPSAMREVAIDVPNVSWSDIGGLENVKLKLKQAVEWPLMHPDSFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAVKGPELMNKYVGESERAVREIFHKARAVAPSILFFDEIDALAIERGSSAGS-VADRVLAQLLTEMDGIEQLK--DVVILAATNRPDLIDKALMRPGRIDRIIYVPLPDAATRREIFKLRFHSMPISTE----ICLEKLVEQTEKYSGAEITAVCRE 784 HSP 3 Score: 110.538 bits (275), Expect = 6.533e-24 Identity = 67/245 (27.35%), Postives = 128/245 (52.24%), Query Frame = 3
Query: 2463 IEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIK-------HGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQ-NVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
I + + T+KE D + + + IGG + + E IE+P+K +G+ P+G+L +GPPG KT+ A+A E + + +I+ Q PS+IFI+++D L PKR +++ V +R+++ L+T + GI ++++Q ++++ TN H++D AL RPGR + IG+P+ + R IL+ + + + E +++ +A+ G GA++ + +E
Sbjct: 287 ITYKEQKTTTAKETDELKRKVTYNMIGGLKAELKEIRETIELPLKQPELFRNYGIPPPRGVLLYGPPGTGKTLIARAIANEVGAHVTVINGPEIVSN-------------EQQCACCPSIIFIDELDALCPKRE-GAQNEVEKRVVASLLTLMDGIGSEESQGQLLVLGATNRPHSLDPALRRPGRFDKEIEIGVPNAQGRLDILQKVLKKVPHRLKEE---DLAQLADRTHGYVGADLAALCKE 514 HSP 4 Score: 93.2041 bits (230), Expect = 1.297e-18 Identity = 67/229 (29.26%), Postives = 110/229 (48.03%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPN---VIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRR 1679
IGG ++ ++ IE PLK + F +GVL +GPPG KT+ RA+A E G ++ I I+ ++ A PSI+FIDE+D + P R+ A + V +R+++ LL ++GI + + ++ + ATN LD A+ R GR IEIG+P+ R IL +E+ + +A+R G GA++ ++ + R
Sbjct: 312 IGGLKAELKEIRETIELPLKQPELFRNYGIPPPRGVLLYGPPGTGKTLIARAIANEVGAHVTVINGPEIVSN-------------EQQCACCPSIIFIDELDALCPKREG-AQNEVEKRVVASLLTLMDGIGSEESQGQLLVLGATNRPHSLDPALRRPGRFDKEIEIGVPNAQGRLDILQKVLKKVPHRL-----KEEDLAQLADRTHGYVGADLAALCKEAGMNALR 521
BLAST of AAA family ATPase vs. Ensembl Xenopus
Match: SPATA5 (spermatogenesis associated 5 [Source:NCBI gene;Acc:100494828]) HSP 1 Score: 165.622 bits (418), Expect = 3.221e-41 Identity = 95/240 (39.58%), Postives = 136/240 (56.67%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
PS + D+ + W DIGG E+VK+ LK +E PL + F+ KGVL +GPPGCSKTM +ALA E+GLN +++K +M YVGESE +R+IF +A A+ PSILF DEID + R A S V+ R++++LL ++GI + +V+ +AATN D +D A+MR GR+ I + LPD +R I + F + + E+ SGAEI R+ L
Sbjct: 545 PSAMREVAIDVPNVSWSDIGGLENVKLKLKQAVEWPLMHPDSFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAVKGPELMNKYVGESERAVREIFHKARAVAPSILFFDEIDALAIERGSSAGS-VADRVLAQLLTEMDGIEQLKDVVILAATNRPDLIDKALMRPGRIDRIIYVPLPDAATRREIFKLRFHS------MPISTEICLEKLVEQTEKYSGAEITAVCREAAL 777 HSP 2 Score: 162.54 bits (410), Expect = 2.775e-40 Identity = 102/301 (33.89%), Postives = 155/301 (51.50%), Query Frame = 3
Query: 2352 EDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQL-------------------IEFSREIENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
ED+ +L HG+ D+ K A + R R L ++ + E+ ++ +E+ + W+DIGG E VK L + +E P+ H G++ PKG+L +GPPGC KTM ALA E L V ++M VGESE A R I +A +PS++F ++ID L +R S++ S V +R+L++L+T + GIE K +V+I+A TN +D+AL+RPGR+ I+ + LPD R I K+ F + TE + + E SGAEI + RE
Sbjct: 481 EDLAQLADRTHGYVGADLAALCKEAGMNALRRTHRVLSRPSDREMAGSVVITLNDFLQATNEVRPSAMREVAIDVPNVSWSDIGGLENVKLKLKQAVEWPLMHPDSFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAVKGPELMNKYVGESERAVREIFHKARAVAPSILFFDEIDALAIERGSSAGS-VADRVLAQLLTEMDGIEQLK--DVVILAATNRPDLIDKALMRPGRIDRIIYVPLPDAATRREIFKLRFHSMPISTE----ICLEKLVEQTEKYSGAEITAVCRE 774 HSP 3 Score: 110.153 bits (274), Expect = 7.832e-24 Identity = 67/245 (27.35%), Postives = 128/245 (52.24%), Query Frame = 3
Query: 2463 IEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIK-------HGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQ-NVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
I + + T+KE D + + + IGG + + E IE+P+K +G+ P+G+L +GPPG KT+ A+A E + + +I+ Q PS+IFI+++D L PKR +++ V +R+++ L+T + GI ++++Q ++++ TN H++D AL RPGR + IG+P+ + R IL+ + + + E +++ +A+ G GA++ + +E
Sbjct: 277 ITYKEQKTTTAKETDELKRKVTYNMIGGLKAELKEIRETIELPLKQPELFRNYGIPPPRGVLLYGPPGTGKTLIARAIANEVGAHVTVINGPEIVSN-------------EQQCACCPSIIFIDELDALCPKRE-GAQNEVEKRVVASLLTLMDGIGSEESQGQLLVLGATNRPHSLDPALRRPGRFDKEIEIGVPNAQGRLDILQKVLKKVPHRLKEE---DLAQLADRTHGYVGADLAALCKE 504 HSP 4 Score: 93.2041 bits (230), Expect = 1.351e-18 Identity = 67/229 (29.26%), Postives = 109/229 (47.60%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGIN---EKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRR 1679
IGG ++ ++ IE PLK + F +GVL +GPPG KT+ RA+A E G ++ I I+ ++ A PSI+FIDE+D + P R+ A + V +R+++ LL ++GI + ++ + ATN LD A+ R GR IEIG+P+ R IL +E+ + +A+R G GA++ ++ + R
Sbjct: 302 IGGLKAELKEIRETIELPLKQPELFRNYGIPPPRGVLLYGPPGTGKTLIARAIANEVGAHVTVINGPEIVSN-------------EQQCACCPSIIFIDELDALCPKREG-AQNEVEKRVVASLLTLMDGIGSEESQGQLLVLGATNRPHSLDPALRRPGRFDKEIEIGVPNAQGRLDILQKVLKKVPHRL-----KEEDLAQLADRTHGYVGADLAALCKEAGMNALR 511
BLAST of AAA family ATPase vs. Ensembl Mouse
Match: Spata5 (spermatogenesis associated 5 [Source:MGI Symbol;Acc:MGI:1927170]) HSP 1 Score: 166.777 bits (421), Expect = 1.005e-41 Identity = 87/200 (43.50%), Postives = 124/200 (62.00%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR-DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITF 1544
PS + D+ + W DIGG E++K+ LK +E PLK+ K F KGVL +GPPGCSKTM +ALA E+GLN ++IK +M YVGESE +R+IF +A A+ PSI+F DE+D + R V+ R++++LL ++GI + NV +AATN D++D A+MR GR+ I + LPD +R IL + F
Sbjct: 611 PSAMREVAIDVPNVSWSDIGGLENIKLKLKQAVEWPLKHPKSFNRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNKYVGESERAVREIFRKARAVAPSIIFFDELDALAVERGSSSGAGNVADRVLAQLLTEMDGIEQLKNVTVLAATNRPDRIDKALMRPGRIDRIIYVPLPDAATRREILNLQF 810 HSP 2 Score: 158.688 bits (400), Expect = 4.629e-39 Identity = 96/312 (30.77%), Postives = 165/312 (52.88%), Query Frame = 3
Query: 2253 GFVNKKVCMSLP--KDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQ-------------------LIEFSREIENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKS-NVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEI 3098
G +K++ + +P +D + L+ ++ ++ ++ + E + L N HG+ D++ A + R +R+ ++ +I ++ +E+ + W+DIGG E +K L + +E P+KH G++ PKG+L +GPPGC KTM ALA E L + ++M VGESE A R I +A +PS+IF +++D L +R S+S + NV +R+L++L+T + GIE K NV ++A TN +D+AL+RPGR+ I+ + LPD R IL + F + E ++
Sbjct: 513 GRFDKEIEIGIPNAQDRLDILQKLLRRVPHLLTKAE-LLRLANNAHGYVGADLKALCNEAGLHALRRVLRKQPNLPDSKVAGMVKITLNDFLQGMNDIRPSAMREVAIDVPNVSWSDIGGLENIKLKLKQAVEWPLKHPKSFNRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNKYVGESERAVREIFRKARAVAPSIIFFDELDALAVERGSSSGAGNVADRVLAQLLTEMDGIEQLK--NVTVLAATNRPDRIDKALMRPGRIDRIIYVPLPDAATRREILNLQFHSMPISNEVDL 821 HSP 3 Score: 122.094 bits (305), Expect = 1.197e-27 Identity = 71/202 (35.15%), Postives = 117/202 (57.92%), Query Frame = 3
Query: 2475 REIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIK-------HGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQN-VMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILK 3056
R+I SKE D Q + + + IGG + + E IE+P+K +G+ P+G+L +GPPG KTM A+A E + + +I+ GE+EA R I ++A PS+IFI+++D L PKR ++S V +R+++ L+T + GI ++ ++ V+++ TN +D AL RPGR + IG+P+ +DR IL+
Sbjct: 335 RKICTNSKEQDSQFK-VTYDMIGGLNSQLKAIREIIELPLKQPELFKSYGIPAPRGLLLYGPPGTGKTMIARAVANEVGAYVSVINGPEIISKFYGETEARLRQIFAEATLRHPSIIFIDELDALCPKR-EGAQSEVEKRVVASLLTLMDGIGSEGSEGRVLVLGATNRPQALDAALRRPGRFDKEIEIGIPNAQDRLDILQ 534 HSP 4 Score: 117.857 bits (294), Expect = 2.570e-26 Identity = 72/180 (40.00%), Postives = 100/180 (55.56%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPN---VIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
IGG S ++ IE PLK + F +G+L +GPPG KTM RA+A E G + I I+ + GE+E LRQIF A PSI+FIDE+D + P R+ A S V +R+++ LL ++GI + + V+ + ATN LDAA+ R GR IEIG+P+ R IL
Sbjct: 355 IGGLNSQLKAIREIIELPLKQPELFKSYGIPAPRGLLLYGPPGTGKTMIARAVANEVGAYVSVINGPEIISKFYGETEARLRQIFAEATLRHPSIIFIDELDALCPKREG-AQSEVEKRVVASLLTLMDGIGSEGSEGRVLVLGATNRPQALDAALRRPGRFDKEIEIGIPNAQDRLDIL 533
BLAST of AAA family ATPase vs. Ensembl Mouse
Match: Spata5 (spermatogenesis associated 5 [Source:MGI Symbol;Acc:MGI:1927170]) HSP 1 Score: 166.777 bits (421), Expect = 1.369e-41 Identity = 87/200 (43.50%), Postives = 124/200 (62.00%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR-DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITF 1544
PS + D+ + W DIGG E++K+ LK +E PLK+ K F KGVL +GPPGCSKTM +ALA E+GLN ++IK +M YVGESE +R+IF +A A+ PSI+F DE+D + R V+ R++++LL ++GI + NV +AATN D++D A+MR GR+ I + LPD +R IL + F
Sbjct: 610 PSAMREVAIDVPNVSWSDIGGLENIKLKLKQAVEWPLKHPKSFNRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNKYVGESERAVREIFRKARAVAPSIIFFDELDALAVERGSSSGAGNVADRVLAQLLTEMDGIEQLKNVTVLAATNRPDRIDKALMRPGRIDRIIYVPLPDAATRREILNLQF 809 HSP 2 Score: 162.925 bits (411), Expect = 2.216e-40 Identity = 104/340 (30.59%), Postives = 176/340 (51.76%), Query Frame = 3
Query: 2253 GFVNKKVCMSLP--KDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQ-------------------LIEFSREIENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKS-NVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTE---HEIISNISTIAESLTGLSGAEIVGIVRE 3173
G +K++ + +P +D + L+ ++ ++ ++ + E + L N HG+ D++ A + R +R+ ++ +I ++ +E+ + W+DIGG E +K L + +E P+KH G++ PKG+L +GPPGC KTM ALA E L + ++M VGESE A R I +A +PS+IF +++D L +R S+S + NV +R+L++L+T + GIE K NV ++A TN +D+AL+RPGR+ I+ + LPD R IL + F + E E++ T SGAEI+ + +E
Sbjct: 512 GRFDKEIEIGIPNAQDRLDILQKLLRRVPHLLTKAE-LLRLANNAHGYVGADLKALCNEAGLHALRRVLRKQPNLPDSKVAGMVKITLNDFLQGMNDIRPSAMREVAIDVPNVSWSDIGGLENIKLKLKQAVEWPLKHPKSFNRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNKYVGESERAVREIFRKARAVAPSIIFFDELDALAVERGSSSGAGNVADRVLAQLLTEMDGIEQLK--NVTVLAATNRPDRIDKALMRPGRIDRIIYVPLPDAATRREILNLQFHSMPISNEVDLDELVLQTDT-------YSGAEIIAVCKE 841 HSP 3 Score: 122.094 bits (305), Expect = 1.417e-27 Identity = 71/202 (35.15%), Postives = 117/202 (57.92%), Query Frame = 3
Query: 2475 REIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIK-------HGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQN-VMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILK 3056
R+I SKE D Q + + + IGG + + E IE+P+K +G+ P+G+L +GPPG KTM A+A E + + +I+ GE+EA R I ++A PS+IFI+++D L PKR ++S V +R+++ L+T + GI ++ ++ V+++ TN +D AL RPGR + IG+P+ +DR IL+
Sbjct: 334 RKICTNSKEQDSQFK-VTYDMIGGLNSQLKAIREIIELPLKQPELFKSYGIPAPRGLLLYGPPGTGKTMIARAVANEVGAYVSVINGPEIISKFYGETEARLRQIFAEATLRHPSIIFIDELDALCPKR-EGAQSEVEKRVVASLLTLMDGIGSEGSEGRVLVLGATNRPQALDAALRRPGRFDKEIEIGIPNAQDRLDILQ 533 HSP 4 Score: 117.857 bits (294), Expect = 2.653e-26 Identity = 72/180 (40.00%), Postives = 100/180 (55.56%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPN---VIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
IGG S ++ IE PLK + F +G+L +GPPG KTM RA+A E G + I I+ + GE+E LRQIF A PSI+FIDE+D + P R+ A S V +R+++ LL ++GI + + V+ + ATN LDAA+ R GR IEIG+P+ R IL
Sbjct: 354 IGGLNSQLKAIREIIELPLKQPELFKSYGIPAPRGLLLYGPPGTGKTMIARAVANEVGAYVSVINGPEIISKFYGETEARLRQIFAEATLRHPSIIFIDELDALCPKREG-AQSEVEKRVVASLLTLMDGIGSEGSEGRVLVLGATNRPQALDAALRRPGRFDKEIEIGIPNAQDRLDIL 532
BLAST of AAA family ATPase vs. Ensembl Mouse
Match: Spata5 (spermatogenesis associated 5 [Source:MGI Symbol;Acc:MGI:1927170]) HSP 1 Score: 166.777 bits (421), Expect = 1.436e-41 Identity = 87/200 (43.50%), Postives = 124/200 (62.00%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR-DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITF 1544
PS + D+ + W DIGG E++K+ LK +E PLK+ K F KGVL +GPPGCSKTM +ALA E+GLN ++IK +M YVGESE +R+IF +A A+ PSI+F DE+D + R V+ R++++LL ++GI + NV +AATN D++D A+MR GR+ I + LPD +R IL + F
Sbjct: 611 PSAMREVAIDVPNVSWSDIGGLENIKLKLKQAVEWPLKHPKSFNRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNKYVGESERAVREIFRKARAVAPSIIFFDELDALAVERGSSSGAGNVADRVLAQLLTEMDGIEQLKNVTVLAATNRPDRIDKALMRPGRIDRIIYVPLPDAATRREILNLQF 810 HSP 2 Score: 162.925 bits (411), Expect = 2.187e-40 Identity = 104/340 (30.59%), Postives = 176/340 (51.76%), Query Frame = 3
Query: 2253 GFVNKKVCMSLP--KDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQ-------------------LIEFSREIENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKS-NVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTE---HEIISNISTIAESLTGLSGAEIVGIVRE 3173
G +K++ + +P +D + L+ ++ ++ ++ + E + L N HG+ D++ A + R +R+ ++ +I ++ +E+ + W+DIGG E +K L + +E P+KH G++ PKG+L +GPPGC KTM ALA E L + ++M VGESE A R I +A +PS+IF +++D L +R S+S + NV +R+L++L+T + GIE K NV ++A TN +D+AL+RPGR+ I+ + LPD R IL + F + E E++ T SGAEI+ + +E
Sbjct: 513 GRFDKEIEIGIPNAQDRLDILQKLLRRVPHLLTKAE-LLRLANNAHGYVGADLKALCNEAGLHALRRVLRKQPNLPDSKVAGMVKITLNDFLQGMNDIRPSAMREVAIDVPNVSWSDIGGLENIKLKLKQAVEWPLKHPKSFNRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNKYVGESERAVREIFRKARAVAPSIIFFDELDALAVERGSSSGAGNVADRVLAQLLTEMDGIEQLK--NVTVLAATNRPDRIDKALMRPGRIDRIIYVPLPDAATRREILNLQFHSMPISNEVDLDELVLQTDT-------YSGAEIIAVCKE 842 HSP 3 Score: 122.094 bits (305), Expect = 1.407e-27 Identity = 71/202 (35.15%), Postives = 117/202 (57.92%), Query Frame = 3
Query: 2475 REIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIK-------HGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQN-VMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILK 3056
R+I SKE D Q + + + IGG + + E IE+P+K +G+ P+G+L +GPPG KTM A+A E + + +I+ GE+EA R I ++A PS+IFI+++D L PKR ++S V +R+++ L+T + GI ++ ++ V+++ TN +D AL RPGR + IG+P+ +DR IL+
Sbjct: 335 RKICTNSKEQDSQFK-VTYDMIGGLNSQLKAIREIIELPLKQPELFKSYGIPAPRGLLLYGPPGTGKTMIARAVANEVGAYVSVINGPEIISKFYGETEARLRQIFAEATLRHPSIIFIDELDALCPKR-EGAQSEVEKRVVASLLTLMDGIGSEGSEGRVLVLGATNRPQALDAALRRPGRFDKEIEIGIPNAQDRLDILQ 534 HSP 4 Score: 117.857 bits (294), Expect = 2.774e-26 Identity = 72/180 (40.00%), Postives = 100/180 (55.56%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPN---VIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
IGG S ++ IE PLK + F +G+L +GPPG KTM RA+A E G + I I+ + GE+E LRQIF A PSI+FIDE+D + P R+ A S V +R+++ LL ++GI + + V+ + ATN LDAA+ R GR IEIG+P+ R IL
Sbjct: 355 IGGLNSQLKAIREIIELPLKQPELFKSYGIPAPRGLLLYGPPGTGKTMIARAVANEVGAYVSVINGPEIISKFYGETEARLRQIFAEATLRHPSIIFIDELDALCPKREG-AQSEVEKRVVASLLTLMDGIGSEGSEGRVLVLGATNRPQALDAALRRPGRFDKEIEIGIPNAQDRLDIL 533
BLAST of AAA family ATPase vs. Ensembl Mouse
Match: Vcp (valosin containing protein [Source:MGI Symbol;Acc:MGI:99919]) HSP 1 Score: 153.68 bits (387), Expect = 1.233e-37 Identity = 130/463 (28.08%), Postives = 217/463 (46.87%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-SNLNEEFH------------LIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF-----IDNVYRKFKEITLE----------RLKACRKDSNWI-----PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR--DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVE 1646
++++GP G GK + + +A+E FF IN L +E + K + A K+ ++ +++ + E+ H ++D L + + + + PNS RR +EV + I D R E+ + + M DD + ++ + N+ + G DL + + +K I LE L D W PS L + ++ + W DIGG E VK L+ ++ P+++ +F+K GVLF+GPPGC KT+ +A+A E N ISIK ++ + GESE +R+IFD+A P +LF DE+D I +R + + R+++++L ++G++ K NV + ATN D +D A++R GR+ I I LPD+ SR +IL A DV F +A+ +G SGA++ E
Sbjct: 241 ILLYGPPGTGKTLIARAVANET-GAFFFLINGPEIMSKLAGESESNLRKAFEE---AEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQI---HTKNMKLADDVDLEQVA-NETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSP----VAKDVDLEF--LAKMTNGFSGADLTE 689 HSP 2 Score: 140.969 bits (354), Expect = 1.474e-33 Identity = 106/373 (28.42%), Postives = 176/373 (47.18%), Query Frame = 3
Query: 2223 DLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA---------------QFHNNNENV--SDSLPTIKYQYFREAI 3263
DL+ V ++ G V + +E L+ + K+ I E I+ V N T D RW L S+++ L E E+ + W DIGG E VK L E ++ P++H G+ KG+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R I +A +++P ++F +++D + R + +R++++++T + G+ TKK NV I+ TN +D A+LRPGRL ++ I LPD+K R +ILK + ++ +A+ G SGA++ I + + A Q + + V D +P I+ +F EA+
Sbjct: 395 DLEQVANETHGHVGADLAALC---SEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWAL-------SQSNPSALRETVVEVPQVT-----------WEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKK--NVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKD----VDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAM 740 HSP 3 Score: 129.413 bits (324), Expect = 5.819e-30 Identity = 81/239 (33.89%), Postives = 125/239 (52.30%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRALPL 1691
L + + DIGG +K +E PL++ G + +G+L +GPPG KT+ RA+A ETG I IM GESE LR+ F+ A P+I+FIDE+D I P R+ V +R++S+LL ++G+ ++ +VI +AATN + +D A+ R GR ++IG+PD R IL I + ADDV + +A G GA++ + L +R+ + L
Sbjct: 198 LNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTH-GEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMK----LADDV--DLEQVANETHGHVGADLAALCSEAALQAIRKKMDL 429 HSP 4 Score: 126.331 bits (316), Expect = 5.213e-29 Identity = 72/227 (31.72%), Postives = 124/227 (54.63%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
+ DIGG + E +E+P++H G++ P+GIL +GPPG KT+ A+A E F + +IM + GESE+ R +A +++P++IFI+++D + PKR + V RI+S+L+T + G+ K+ +V+++A TN +++D AL R GR V IG+PD R IL+I ++ + ++ +A G GA++ + E +A
Sbjct: 203 YDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKR-EKTHGEVERRIVSQLLTLMDGL--KQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADD----VDLEQVANETHGHVGADLAALCSEAALQA 422
BLAST of AAA family ATPase vs. Ensembl Mouse
Match: Nvl (nuclear VCP-like [Source:MGI Symbol;Acc:MGI:1914709]) HSP 1 Score: 140.198 bits (352), Expect = 2.651e-33 Identity = 83/224 (37.05%), Postives = 126/224 (56.25%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESL--TGLSGAEIVGIVRE 3173
W DIG E ++ LI I P+++ GL P GIL GPPGC KT+ A+A E L +V +++ VGESE A R + +A S+P +IF +++D L P+R S+ ++ R++++L+T + G+ET+ Q V I+A TN +D A+LRPGRL + +GLP DR +ILK + E + N+ TIA L +GA++ +VRE
Sbjct: 579 WADIGALEDIRDELIMAILAPVRNPDQFRTLGLGTPAGILLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRAKNSAPCVIFFDEVDALCPRR-SDRETGASVRVVNQLLTEMDGLETR--QQVFILAATNRPDIIDPAILRPGRLDKTLFVGLPPPADRVAILKTITKNGTKPPLDEDV-NLETIANDLRCNCYTGADLTALVRE 798 HSP 2 Score: 136.346 bits (342), Expect = 4.251e-32 Identity = 80/231 (34.63%), Postives = 122/231 (52.81%), Query Frame = 3
Query: 1014 WVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAE--RLSGKSGAEIVEFGRQVRLGVRR 1679
W DIG E ++ L I P++N +F G+L GPPGC KT+ +A+A E+GLN IS+K ++ YVGESE +RQ+F RA P ++F DE+D + P R S R++++LL ++G+ + V +AATN D +D A++R GR+ + +GLP R +IL T + +DV N IA R + +GA++ R+ L R
Sbjct: 579 WADIGALEDIRDELIMAILAPVRNPDQFRTLGLGTPAGILLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRAKNSAPCVIFFDEVDALCPRRSDRETG-ASVRVVNQLLTEMDGLETRQQVFILAATNRPDIIDPAILRPGRLDKTLFVGLPPPADRVAILK-TITKNGTKPPLDEDV--NLETIANDLRCNCYTGADLTALVREASLCALR 805 HSP 3 Score: 124.79 bits (312), Expect = 1.812e-28 Identity = 73/199 (36.68%), Postives = 109/199 (54.77%), Query Frame = 3
Query: 1101 KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMF---LNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRAL 1685
+GVL HGPPGC KT+ A+A E L ++ + I+ G GESE LR++FD+AV+ P I+FIDEID I P R+ +A + +R++++LL LN + V+ + ATN D LD A+ R+GR + +G+PD+ +RE IL F NF +A G GA+++ R+ + V R L
Sbjct: 298 RGVLLHGPPGCGKTLLAHAIAGELDLPILKVAAPEIVSGVSGESEQKLRELFDQAVSNAPCIVFIDEIDAITPKRE-VASKDMERRIVAQLLTCMDDLNNVAATARVLVIGATNRPDSLDPALRRAGRFDREVCLGIPDEAARERILQTLCRKLRLPETF------NFCHLAHLTPGFVGADLMALCREAAMCAVNRVL 489 HSP 4 Score: 122.479 bits (306), Expect = 9.813e-28 Identity = 73/224 (32.59%), Postives = 120/224 (53.57%), Query Frame = 3
Query: 2523 LHWTDIGGYET----VKTMLI--ENIEMPIKHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGI-ETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
+ + D+GG + V MLI + E+ G+ P+G+L HGPPGC KT+ +A+A E L + V +I+ G+ GESE R + QA+ ++P ++FI++ID + PKR SK ++ RI+++L+T + + V+++ TN ++D AL R GR V +G+PD+ RE IL+ L N +A G GA+++ + RE
Sbjct: 261 VKFEDVGGNDATLKEVCKMLIHMRHPEVYQHLGVVPPRGVLLHGPPGCGKTLLAHAIAGELDLPILKVAAPEIVSGVSGESEQKLRELFDQAVSNAPCIVFIDEIDAITPKREVASK-DMERRIVAQLLTCMDDLNNVAATARVLVIGATNRPDSLDPALRRAGRFDREVCLGIPDEAARERILQTLCRKLRLPETF----NFCHLAHLTPGFVGADLMALCRE 479
BLAST of AAA family ATPase vs. UniProt/SwissProt
Match: sp|P32794|AFG2_YEAST (ATPase family gene 2 protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=AFG2 PE=1 SV=1) HSP 1 Score: 184.882 bits (468), Expect = 6.542e-47 Identity = 139/458 (30.35%), Postives = 226/458 (49.34%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINCLTWFNEKDYLKIIYQTL--IANKSIQMVLCCYFINS-----------------SNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRRR----KEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF--------------IDNVYRKFK-EITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL---GITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIV 1643
+++HGP G GK L++ +A+ N IN + ++ YL L I N++ + FI+ S + ++D + ++ + PNS RR +EV + I D ++RF++ +R + + D E K+ ++ + + G DLT D KF ++TL+ +++ D PS + ++ ++W DIGG E +K +K I+ PL+ + F KGVL +GPPGCSKT+ +ALATE+G+N +++K I YVGESE +R+IF +A + PSI+F DEID + P RD + S + +++ LL ++G+ E V+ VAATN D++DAA++R GR+ HI +G PD +R IL F+ + G D+ E +A+R G SGAE+V
Sbjct: 282 ILLHGPPGTGKTMLLRVVAN-TSNAHVLTINGPSIVSK--YLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAGKVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSEAIKYIASKTHGYVGADLTALCRESVMKTIQRGLGTDANIDKFSLKVTLKDVESAMVDIR--PSAMREIFLEMPKVYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAPKGVLLYGPPGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESERAIREIFRKARSAAPSIIFFDEIDALSPDRDGSSTS-AANHVLTSLLNEIDGVEELKGVVIVAATNRPDEIDAALLRPGRLDRHIYVGPPDVNARLEILKKCTKKFNTEESGV----DLHE----LADRTEGYSGAEVV 725 HSP 2 Score: 148.288 bits (373), Expect = 4.649e-35 Identity = 88/224 (39.29%), Postives = 127/224 (56.70%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
++W+DIGG E +KT + E I++P++ G+ PKG+L +GPPGC KT+ ALATE + V +I VGESE A R I +A ++PS+IF ++ID L P R +S S +L+ L+ + G+E K V+IVA TN +D ALLRPGRL + +G PD R ILK F TE + ++ +A+ G SGAE+V + +E
Sbjct: 513 VYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAPKGVLLYGPPGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESERAIREIFRKARSAAPSIIFFDEIDALSPDRDGSSTS-AANHVLTSLLNEIDGVEELK--GVVIVAATNRPDEIDAALLRPGRLDRHIYVGPPDVNARLEILK--KCTKKFNTEESGV-DLHELADRTEGYSGAEVVLLCQE 730 HSP 3 Score: 140.584 bits (353), Expect = 1.061e-32 Identity = 85/240 (35.42%), Postives = 127/240 (52.92%), Query Frame = 3
Query: 1002 EPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQ-VRLGVRRALPLDT 1697
EPL + +GG + LK+ IE PL F +G+L HGPPG KTM +R +A + ++++I +I+ Y+GE+E LR IF+ A QPSI+FIDEID I P+R + V R+++ LL ++G+ V+ +AATN + +D A+ R GR +EIG+PD +R IL FS + D E IA + G GA++ R+ V ++R L D
Sbjct: 240 EPLSYAAVGGLDKEIESLKSAIEIPLHQPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTINGPSIVSKYLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAGKVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLD--SEAIKYIASKTHGYVGADLTALCRESVMKTIQRGLGTDA 477 HSP 4 Score: 132.494 bits (332), Expect = 4.234e-30 Identity = 96/340 (28.24%), Postives = 157/340 (46.18%), Query Frame = 3
Query: 2229 KLVPSQLDGF-----VNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTD-------------MRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
K+ L G+ + +KV L D+ + MI++ L K+ E I+ ++ + + D+ + Y D I FS+E + K + +PL + +GG + L IE+P+ G+ P+GIL HGPPG KTM + +A + + I+ +GE+EAA R I ++A + PS+IFI++ID + P R+++ V R+++ L+T + G+ V+++A TN ++VD AL RPGR V IG+PD R IL FS ++ I IA G GA++ + RE
Sbjct: 128 KVTVGSLQGYNILECMEEKVIQKLLDDSGVIMPGMIFQNLKTKAGDESIDVVITDASDDSLPDVSQLDLNMDDMYGGLDNLFYLSPPFIFRKGSTHITFSKETQANRKY--NLPEPLSYAAVGGLDKEIESLKSAIEIPLHQPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTINGPSIVSKYLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAG--KVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSEAIKYIASKTHGYVGADLTALCRE 463
BLAST of AAA family ATPase vs. UniProt/SwissProt
Match: sp|O60058|AFG2_SCHPO (ATPase family gene 2 protein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=afg2 PE=3 SV=1) HSP 1 Score: 182.956 bits (463), Expect = 3.971e-46 Identity = 139/465 (29.89%), Postives = 235/465 (50.54%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINCLT----WFNEKD-YLKIIYQTLIANKS----IQMVLCCYFINSSNLNEE-------FHLIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDN-FDDPEWKKFSDNQLNQFTGKDLTDFIDNVYRKFKEITLERLKACRKDSNWI-------------------PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
V+++GP G GK +M+ +A E N F I+ + + E + L+ I++ A++ I + + +++E ++D + ++ + PNS RR KE+ + I DK++R ++ K L+ +G+ N +D + + + ++ + + G DL V R+ ++R + +KD++ + S + ++ + +HW DIGG E VK LK +E PL +G+ F KGVL +GPPGCSKT+ +A+ATETGLN I++K + +VGESE +RQ+F +A PS++F DEID + +R S R+++ LL L+GI NV+ +AATN D +D A+MR GR+ + +G P+ +R+ I+ I Q + FA+DV + IAE+ G SGAE+V ++ L
Sbjct: 316 VLLYGPPGTGKTMVMRAVAAEA-NAQVFTIDGPSVVGKYLGETESRLRKIFEDARAHQPSIIFIDEIDALAPKRTEDVSEAESRAVATLLTLLDGMANAGKVVVIAATNRPNSIDEALRRPGRLEKEIEIGIPDKSARLDIIKLLL---SGVPNEINDAQLEDLA-SRTHAYVGADLA----AVVREAALRAIKRTISLQKDTSGLDIFGAVQMDDLEFALSSVRQSAMREFMMESPNVHWSDIGGQEEVKQKLKESVEWPLTHGETFSRLGVRPPKGVLLYGPPGCSKTITAKAIATETGLNFIAVKGPELFDKFVGESERAVRQVFQKARQASPSVIFFDEIDALTANRGE---DNSSDRVVAALLNELDGIEALRNVLVLAATNRPDMIDPALMRPGRLDRLLYVGPPNFEARKQIVKI----QAEKMKFAEDV--DLDLIAEKTEGCSGAEVVALCQEAGL 762 HSP 2 Score: 159.844 bits (403), Expect = 9.898e-39 Identity = 120/387 (31.01%), Postives = 200/387 (51.68%), Query Frame = 3
Query: 2139 KMSDDFCEA------VQLTLINEAPNV-KVMMIFEDLKLVPSQLD------GFVNKKVCMSLPKDTEECLKHMIYKIL--NIKSSVED--IEELVKNLHGFTPKDIRWFLKSASVKY-SRT-----DMRQL----------IEF--SREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKHG-------LEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
K ++D EA LTL++ N KV++I + P+ +D G + K++ + +P ++ + I K+L + + + D +E+L H + D+ ++ A+++ RT D L +EF S ++ +E +S +HW+DIGG E VK L E++E P+ HG + PKG+L +GPPGC KT+ A+ATE L V ++ VGESE A R + +A ++SPS+IF ++ID L R + N +R+++ L+ L GIE + NV+++A TN +D AL+RPGRL ++ +G P+ + R+ I+KI + F + ++ IAE G SGAE+V + +E
Sbjct: 387 KRTEDVSEAESRAVATLLTLLDGMANAGKVVVIAATNR--PNSIDEALRRPGRLEKEIEIGIP---DKSARLDIIKLLLSGVPNEINDAQLEDLASRTHAYVGADLAAVVREAALRAIKRTISLQKDTSGLDIFGAVQMDDLEFALSSVRQSAMREFMMESPNVHWSDIGGQEEVKQKLKESVEWPLTHGETFSRLGVRPPKGVLLYGPPGCSKTITAKAIATETGLNFIAVKGPELFDKFVGESERAVRQVFQKARQASPSVIFFDEIDALTANRGED---NSSDRVVAALLNELDGIEALR--NVLVLAATNRPDMIDPALMRPGRLDRLLYVGPPNFEARKQIVKIQAEKMKFAED----VDLDLIAEKTEGCSGAEVVALCQE 759 HSP 3 Score: 130.183 bits (326), Expect = 2.377e-29 Identity = 80/235 (34.04%), Postives = 126/235 (53.62%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFS----DQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRALPL 1691
IGG ++ +++ +E P +N + F +GVL +GPPG KTM +RA+A E + +I +++ Y+GE+E LR+IF+ A A QPSI+FIDEID + P R VS R ++ LL L+G+ V+ +AATN + +D A+ R GR+ IEIG+PD+ +R I+ + S + ND +A R GA++ R+ L ++R + L
Sbjct: 281 IGGLQAQIAQIRDIVELPFQNPELFKFFNIMPPRGVLLYGPPGTGKTMVMRAVAAEANAQVFTIDGPSVVGKYLGETESRLRKIFEDARAHQPSIIFIDEIDALAPKRTE-DVSEAESRAVATLLTLLDGMANAGKVVVIAATNRPNSIDEALRRPGRLEKEIEIGIPDKSARLDIIKLLLSGVPNEINDA---------QLEDLASRTHAYVGADLAAVVREAALRAIKRTISL 505 HSP 4 Score: 124.405 bits (311), Expect = 1.468e-27 Identity = 90/322 (27.95%), Postives = 160/322 (49.69%), Query Frame = 3
Query: 2271 VCMSLPKDTEEC--LKHMIYKILNIK-SSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSR------EIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
V S PK TE+ L YK+ I S ++ E + ++ P+ I F ++S++ D+ +L R + ++ D + ++ IGG + + + +E+P ++ + P+G+L +GPPG KTM + A+A E ++F + ++ +GE+E+ R I A PS+IFI++ID L PKR+ + S R ++ L+T L G+ V+++A TN +++DEAL RPGRL + IG+PD+ R I+K+ S + + + + +A GA++ +VRE +A
Sbjct: 184 VNESEPKATEDLDELSPGSYKVKEIHIRSPSNLIEAISDMSLDEPR-IYKFTAASSMEIETPDLLKLPHEDRTQSAYNQGSEETQNFDGPPSAVTFSSIGGLQAQIAQIRDIVELPFQNPELFKFFNIMPPRGVLLYGPPGTGKTMVMRAVAAEANAQVFTIDGPSVVGKYLGETESRLRKIFEDARAHQPSIIFIDEIDALAPKRTEDV-SEAESRAVATLLTLLDGMANAG--KVVVIAATNRPNSIDEALRRPGRLEKEIEIGIPDKSARLDIIKLLLSGVPNEIND---AQLEDLASRTHAYVGADLAAVVREAALRA 498
BLAST of AAA family ATPase vs. UniProt/SwissProt
Match: sp|Q21222|AFG2H_CAEEL (ATPase family protein 2 homolog OS=Caenorhabditis elegans OX=6239 GN=cdc-48.3 PE=1 SV=1) HSP 1 Score: 171.785 bits (434), Expect = 8.430e-43 Identity = 98/313 (31.31%), Postives = 172/313 (54.95%), Query Frame = 3
Query: 2364 ELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHNNNENVSDSLPTIKYQYFREAISASCS 3278
++ ++ HGFT D+ LK+A RT + ++ + + I T ++ + + W DIGG E +K + + + P KH G++ P GIL +GPPGC KT+ ALA+E ++ V ++ VG+SE A R + S+A + +P+++F ++ID + R S S V +R+L++L+T L G+E K+ V+++A TN +D ALLRPGRL + +GLP + R +IL++ + F + + I + E +G SGAE+V + R A E++ ++ +++ +F +A++A S
Sbjct: 402 DVARHTHGFTGGDLCSLLKAAKFARGRTHLERVNDARKRIRPTGIRQFILEVPNVSWNDIGGNEELKLEIQQAVIWPQKHPEAFERFGIDPPAGILLYGPPGCSKTLIARALASEAKMNFLAVKGPELFSKWVGDSEKAIRDLFSRARQVAPTIVFFDEIDAVGSSRGSEKSSGVSDRVLAQLLTELDGLE--KSSRVILLAATNRPDQLDSALLRPGRLDRAIYVGLPCEVTRRAILEMRTKKMKFD---DTVRTIDKLVEKTSGYSGAELVAVC----RTAAMFAMRESIDATI--VQWTHFEQALAAVVS 703 HSP 2 Score: 169.859 bits (429), Expect = 3.627e-42 Identity = 98/279 (35.13%), Postives = 154/279 (55.20%), Query Frame = 3
Query: 864 FTGKDLTDFIDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRR 1679
FTG DL + LER+ RK P+ + +I ++ + W DIGG E +K+ ++ + P K+ + F + G+L +GPPGCSKT+ RALA+E +N +++K + +VG+SE +R +F RA + P+I+F DEID + SR S VS R++++LL L+G+ + VI +AATN D+LD+A++R GR+ I +GLP +V+R +IL + + F DD + E+ SG SGAE+V R + R
Sbjct: 410 FTGGDLCSLLKAAKFARGRTHLERVNDARKRIR--PTGIRQFILEVPNVSWNDIGGNEELKLEIQQAVIWPQKHPEAFERFGIDPPAGILLYGPPGCSKTLIARALASEAKMNFLAVKGPELFSKWVGDSEKAIRDLFSRARQVAPTIVFFDEIDAVGSSRGSEKSSGVSDRVLAQLLTELDGLEKSSRVILLAATNRPDQLDSALLRPGRLDRAIYVGLPCEVTRRAILEM----RTKKMKF-DDTVRTIDKLVEKTSGYSGAELVAVCRTAAMFAMR 681
BLAST of AAA family ATPase vs. UniProt/SwissProt
Match: sp|O28972|Y1297_ARCFU (Cell division cycle protein 48 homolog AF_1297 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=AF_1297 PE=3 SV=1) HSP 1 Score: 166.007 bits (419), Expect = 6.417e-41 Identity = 133/460 (28.91%), Postives = 222/460 (48.26%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINCL----TWFNEKDY-LKIIYQTLIANKSIQMVLCCYFINSSNLNEE-------------FHLIIDNLVENSQIDIKILLEIPNSDSVD--FRRR----KEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF-----IDNVYRKFKEI----------TLERLKACRKD-----SNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEI 1640
V+++GP G GK + K +A+E+ + F I+ ++ E + L+ I++ A ++ ++ I+S E ++D L ++ D+ ++ D++D RR +E+ + + DK R E+ + + ++ D E + + N F G DL + + R EI +E LK R+D N PS + + ++ + W DIGG E K L +E PLK + F +G+L GPPG KT+ +A+A E+ N IS+K ++ +VGESE +R++F +A + P ++F DEID + P R + S V++R++S+LL L+G+ E +V+ +AATN D +D A++R GR+ HI I PD+ +R I I G ADDV N +AE+ G SGA+I
Sbjct: 219 VLLYGPPGTGKTLIAKAVANEV-DAHFIPISGPEIMSKYYGESEQRLREIFEE--AKENAPSIIFIDEIDSIAPKREEVTGEVERRVVAQLLALMDGL--EARGDVIVIAATNRPDAIDPALRRPGRFDREIEIGVPDKEGRKEILEIHTRKMPLAEDVDLEELAELT----NGFVGADLEALCKEAAMHALRRVLPEIDIEAEEIPAEVIENLKVTREDFMEALKNIEPSAMREVLVEVPNVKWEDIGGLEHAKQELMEAVEWPLKYPEVFRAANIKPPRGILLFGPPGTGKTLLAKAVANESNANFISVKGPELLSKWVGESEKHVREMFRKARQVAPCVIFFDEIDSLAPRRGGIGDSHVTERVVSQLLTELDGLEELKDVVVIAATNRPDMIDPALLRPGRLERHIYIPPPDKKARVEIFKIHLR----GKPLADDV--NIEELAEKTEGYSGADI 663 HSP 2 Score: 146.362 bits (368), Expect = 1.305e-34 Identity = 99/303 (32.67%), Postives = 155/303 (51.16%), Query Frame = 3
Query: 2355 DIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQL--------------IEFSRE--------IENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
D+EEL + +GF D+ K A++ R + ++ ++ +RE IE ++ +E+ + + W DIGG E K L+E +E P+K+ ++ P+GIL GPPG KT+ A+A E +V +++ VGESE R + +A + +P +IF ++ID L P+R S+V ER++S+L+T L G+E K +V+++A TN +D ALLRPGRL + I PD+K R I KI + NI +AE G SGA+I + RE
Sbjct: 373 DLEELAELTNGFVGADLEALCKEAAMHALRRVLPEIDIEAEEIPAEVIENLKVTREDFMEALKNIEPSAMREVLVEVPNVKWEDIGGLEHAKQELMEAVEWPLKYPEVFRAANIKPPRGILLFGPPGTGKTLLAKAVANESNANFISVKGPELLSKWVGESEKHVREMFRKARQVAPCVIFFDEIDSLAPRRGGIGDSHVTERVVSQLLTELDGLEELK--DVVVIAATNRPDMIDPALLRPGRLERHIYIPPPDKKARVEIFKIHLRGKPLADD----VNIEELAEKTEGYSGADIEAVCRE 669 HSP 3 Score: 144.05 bits (362), Expect = 6.695e-34 Identity = 89/233 (38.20%), Postives = 131/233 (56.22%), Query Frame = 3
Query: 1014 WVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRALP 1688
+ DIGG + +++ IE PLK+ G E KGVL +GPPG KT+ +A+A E + I I IM Y GESE LR+IF+ A PSI+FIDEID I P R+ + V +R++++LL ++G+ + +VI +AATN D +D A+ R GR IEIG+PD+ R+ IL I A+DV + +AE +G GA++ ++ + +RR LP
Sbjct: 181 YEDIGGLKRELRLVREMIELPLKHPELFQRLGIEPPKGVLLYGPPGTGKTLIAKAVANEVDAHFIPISGPEIMSKYYGESEQRLREIFEEAKENAPSIIFIDEIDSIAPKREEV-TGEVERRVVAQLLALMDGLEARGDVIVIAATNRPDAIDPALRRPGRFDREIEIGVPDKEGRKEILEI----HTRKMPLAEDV--DLEELAELTNGFVGADLEALCKEAAMHALRRVLP 406 HSP 4 Score: 136.732 bits (343), Expect = 1.394e-31 Identity = 77/237 (32.49%), Postives = 130/237 (54.85%), Query Frame = 3
Query: 2484 ENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
E ++E+ + + DIGG + ++ E IE+P+KH G+E PKG+L +GPPG KT+ A+A E + +IM GESE R I +A E++PS+IFI++ID + PKR + V R++++L+ + G+E + +V+++A TN +D AL RPGR + IG+PD++ R+ IL+I + + ++ +AE G GA++ + +E
Sbjct: 166 EKPAEEVKRAVPDVTYEDIGGLKRELRLVREMIELPLKHPELFQRLGIEPPKGVLLYGPPGTGKTLIAKAVANEVDAHFIPISGPEIMSKYYGESEQRLREIFEEAKENAPSIIFIDEIDSIAPKREEVT-GEVERRVVAQLLALMDGLEAR--GDVIVIAATNRPDAIDPALRRPGRFDREIEIGVPDKEGRKEILEIHTRKMPLAED----VDLEELAELTNGFVGADLEALCKE 395
BLAST of AAA family ATPase vs. UniProt/SwissProt
Match: sp|Q3UMC0|AFG2H_MOUSE (ATPase family protein 2 homolog OS=Mus musculus OX=10090 GN=Spata5 PE=1 SV=2) HSP 1 Score: 166.777 bits (421), Expect = 1.005e-40 Identity = 87/200 (43.50%), Postives = 124/200 (62.00%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR-DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITF 1544
PS + D+ + W DIGG E++K+ LK +E PLK+ K F KGVL +GPPGCSKTM +ALA E+GLN ++IK +M YVGESE +R+IF +A A+ PSI+F DE+D + R V+ R++++LL ++GI + NV +AATN D++D A+MR GR+ I + LPD +R IL + F
Sbjct: 611 PSAMREVAIDVPNVSWSDIGGLENIKLKLKQAVEWPLKHPKSFNRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNKYVGESERAVREIFRKARAVAPSIIFFDELDALAVERGSSSGAGNVADRVLAQLLTEMDGIEQLKNVTVLAATNRPDRIDKALMRPGRIDRIIYVPLPDAATRREILNLQF 810 HSP 2 Score: 162.925 bits (411), Expect = 1.529e-39 Identity = 104/340 (30.59%), Postives = 176/340 (51.76%), Query Frame = 3
Query: 2253 GFVNKKVCMSLP--KDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQ-------------------LIEFSREIENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKS-NVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTE---HEIISNISTIAESLTGLSGAEIVGIVRE 3173
G +K++ + +P +D + L+ ++ ++ ++ + E + L N HG+ D++ A + R +R+ ++ +I ++ +E+ + W+DIGG E +K L + +E P+KH G++ PKG+L +GPPGC KTM ALA E L + ++M VGESE A R I +A +PS+IF +++D L +R S+S + NV +R+L++L+T + GIE K NV ++A TN +D+AL+RPGR+ I+ + LPD R IL + F + E E++ T SGAEI+ + +E
Sbjct: 513 GRFDKEIEIGIPNAQDRLDILQKLLRRVPHLLTKAE-LLRLANNAHGYVGADLKALCNEAGLHALRRVLRKQPNLPDSKVAGMVKITLNDFLQGMNDIRPSAMREVAIDVPNVSWSDIGGLENIKLKLKQAVEWPLKHPKSFNRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNKYVGESERAVREIFRKARAVAPSIIFFDELDALAVERGSSSGAGNVADRVLAQLLTEMDGIEQLK--NVTVLAATNRPDRIDKALMRPGRIDRIIYVPLPDAATRREILNLQFHSMPISNEVDLDELVLQTDT-------YSGAEIIAVCKE 842 HSP 3 Score: 122.094 bits (305), Expect = 9.842e-27 Identity = 71/202 (35.15%), Postives = 117/202 (57.92%), Query Frame = 3
Query: 2475 REIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIK-------HGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQN-VMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILK 3056
R+I SKE D Q + + + IGG + + E IE+P+K +G+ P+G+L +GPPG KTM A+A E + + +I+ GE+EA R I ++A PS+IFI+++D L PKR ++S V +R+++ L+T + GI ++ ++ V+++ TN +D AL RPGR + IG+P+ +DR IL+
Sbjct: 335 RKICTNSKEQDSQFK-VTYDMIGGLNSQLKAIREIIELPLKQPELFKSYGIPAPRGLLLYGPPGTGKTMIARAVANEVGAYVSVINGPEIISKFYGETEARLRQIFAEATLRHPSIIFIDELDALCPKR-EGAQSEVEKRVVASLLTLMDGIGSEGSEGRVLVLGATNRPQALDAALRRPGRFDKEIEIGIPNAQDRLDILQ 534 HSP 4 Score: 117.857 bits (294), Expect = 1.940e-25 Identity = 72/180 (40.00%), Postives = 100/180 (55.56%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPN---VIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
IGG S ++ IE PLK + F +G+L +GPPG KTM RA+A E G + I I+ + GE+E LRQIF A PSI+FIDE+D + P R+ A S V +R+++ LL ++GI + + V+ + ATN LDAA+ R GR IEIG+P+ R IL
Sbjct: 355 IGGLNSQLKAIREIIELPLKQPELFKSYGIPAPRGLLLYGPPGTGKTMIARAVANEVGAYVSVINGPEIISKFYGETEARLRQIFAEATLRHPSIIFIDELDALCPKREG-AQSEVEKRVVASLLTLMDGIGSEGSEGRVLVLGATNRPQALDAALRRPGRFDKEIEIGIPNAQDRLDIL 533
BLAST of AAA family ATPase vs. TrEMBL
Match: A0A497L5L5 (AAA family ATPase OS=Candidatus Bathyarchaeota archaeon OX=2026714 GN=DRO42_02030 PE=4 SV=1) HSP 1 Score: 186.037 bits (471), Expect = 5.664e-45 Identity = 141/473 (29.81%), Postives = 230/473 (48.63%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWIN---CLTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-------------SNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRRR----KEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF---------------IDNVYRKFKEITLERLKACRKD-----SNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRD-HLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRRALP 1688
V++HGP GCGK L + +A+E + +F+ IN ++ F + ++ A K+ +L I++ + + +D L + + P++ RR +E+ + I D++ RFE+ + + GM +D + K+ ++ + +TG DL ID ++ L++++ +D P+ + + +HW D+GG E VK L+ +E PLK + F KG+L HGPPGC KT+ RA+ATE+ N IS++ + +VGESE +R+IF +A PSI+F DE D + PSR +A S V++R++S+LL ++G+ NV+ +AATN D +D AV+R GR I + PD+ +R IL I D ADDV + +A+A RL+G SGA+I R+ L R P
Sbjct: 221 VLLHGPPGCGKTLLARAVANES-DANFYAINGPEIMSKFYGESEARMRKMFEDAEKNAPSILFIDEIDAIAPKRGEVTGEVERRVVAQLLASMDGLKGRGNVIVIGATNRPDALDPALRRPGRFDREIEIGIPDRDGRFEILQI---HTRGMPLAEDVDLKRIAEIT-HGYTGADLAALCREAAMKALRRYLPQIDLEEKRIPHEVLDQMEVTNEDILDAFREVTPTAMREVFIETPNVHWDDVGGLEDVKQSLREAVEWPLKKPEMFRRLGITPPKGILLHGPPGCGKTLLARAVATESEANFISVRGPEVFSKWVGESEKAIREIFRKARMAAPSIIFFDEFDALVPSRGMGIADSRVTERVISQLLTEIDGLLTLQNVLVLAATNRPDIIDPAVLRPGRFDRRIYVPPPDEEARLKILEIKTKDMP----LADDV--DLAALARRLAGYSGADIDAVCREAALNALRRDP 682 HSP 2 Score: 165.622 bits (418), Expect = 2.802e-38 Identity = 100/304 (32.89%), Postives = 162/304 (53.29%), Query Frame = 3
Query: 2355 DIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQL----------------------IEFSREIENTS-KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSN-SKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
D++ + + HG+T D+ + A++K R + Q+ ++ RE+ T+ +E+ ++ +HW D+GG E VK L E +E P+K G+ PKGIL HGPPGC KT+ A+ATE + +V ++ VGESE A R I +A ++PS+IF ++ D L+P R + S V ER++S+L+T + G+ T QNV+++A TN +D A+LRPGR + + PD++ R IL+I D+ + +++ +A L G SGA+I + RE
Sbjct: 375 DLKRIAEITHGYTGADLAALCREAAMKALRRYLPQIDLEEKRIPHEVLDQMEVTNEDILDAFREVTPTAMREVFIETPNVHWDDVGGLEDVKQSLREAVEWPLKKPEMFRRLGITPPKGILLHGPPGCGKTLLARAVATESEANFISVRGPEVFSKWVGESEKAIREIFRKARMAAPSIIFFDEFDALVPSRGMGIADSRVTERVISQLLTEIDGLLTL--QNVLVLAATNRPDIIDPAVLRPGRFDRRIYVPPPDEEARLKILEIKTKDMPLADD----VDLAALARRLAGYSGADIDAVCRE 672 HSP 3 Score: 150.214 bits (378), Expect = 2.335e-33 Identity = 91/233 (39.06%), Postives = 130/233 (55.79%), Query Frame = 3
Query: 1014 WVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRALP 1688
+ DIGG + ++ +E PL++ G + KGVL HGPPGC KT+ RA+A E+ N +I IM + GESE +R++F+ A PSILFIDEID I P R + V +R++++LL ++G+ + NVI + ATN D LD A+ R GR IEIG+PD+ R IL I G A+DV + IAE G +GA++ R+ + +RR LP
Sbjct: 183 YEDIGGLDEEIKRIREMVELPLRHPEIFQRLGIDPPKGVLLHGPPGCGKTLLARAVANESDANFYAINGPEIMSKFYGESEARMRKMFEDAEKNAPSILFIDEIDAIAPKRGEV-TGEVERRVVAQLLASMDGLKGRGNVIVIGATNRPDALDPALRRPGRFDREIEIGIPDRDGRFEILQI----HTRGMPLAEDV--DLKRIAEITHGYTGADLAALCREAAMKALRRYLP 408 HSP 4 Score: 138.272 bits (347), Expect = 1.435e-29 Identity = 76/227 (33.48%), Postives = 124/227 (54.63%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
+ DIGG + + E +E+P++H G++ PKG+L HGPPGC KT+ A+A E + + +IM GESEA R + A +++PS++FI++ID + PKR + V R++++L+ + G+ K NV+++ TN +D AL RPGR + IG+PD+ R IL+I + + ++ IAE G +GA++ + RE KA
Sbjct: 183 YEDIGGLDEEIKRIREMVELPLRHPEIFQRLGIDPPKGVLLHGPPGCGKTLLARAVANESDANFYAINGPEIMSKFYGESEARMRKMFEDAEKNAPSILFIDEIDAIAPKRGEVT-GEVERRVVAQLLASMDGL--KGRGNVIVIGATNRPDALDPALRRPGRFDREIEIGIPDRDGRFEILQIHTRGMPLAED----VDLKRIAEITHGYTGADLAALCREAAMKA 402
BLAST of AAA family ATPase vs. TrEMBL
Match: B5VNT4 (YLR397Cp-like protein (Fragment) OS=Saccharomyces cerevisiae (strain AWRI1631) OX=545124 GN=AWRI1631_124230 PE=3 SV=1) HSP 1 Score: 184.882 bits (468), Expect = 7.414e-45 Identity = 139/458 (30.35%), Postives = 226/458 (49.34%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINCLTWFNEKDYLKIIYQTL--IANKSIQMVLCCYFINS-----------------SNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF--------------IDNVYRKFK-EITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL---GITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIV 1643
+++HGP G GK L++ +A+ N IN + ++ YL L I N++ + FI+ S + ++D + ++ + PNS RR +EV + I D ++RF++ +R + + D E K+ ++ + + G DLT D KF ++TL+ +++ D PS + ++ ++W DIGG E +K +K I+ PL+ + F KGVL +GPPGCSKT+ +ALATE+G+N +++K I YVGESE +R+IF +A + PSI+F DEID + P RD + S + +++ LL ++G+ E V+ VAATN D++DAA++R GR+ HI +G PD +R IL F+ + G D+ E +A+R G SGAE+V
Sbjct: 173 ILLHGPPGTGKTMLLRVVAN-TSNAHVLTINGPSIVSK--YLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAGKVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSEAIKYIASKTHGYVGADLTALCRESVMKTIQRGLGTDANIDKFSLKVTLKDVESAMVDIR--PSAMREIFLEMPKVYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAPKGVLLYGPPGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESERAIREIFRKARSAAPSIIFFDEIDALSPDRDGSSTS-AANHVLTSLLNEIDGVEELKGVVIVAATNRPDEIDAALLRPGRLDRHIYVGPPDVNARLEILKKCTKKFNTEESGV----DLHE----LADRTEGYSGAEVV 616 HSP 2 Score: 148.673 bits (374), Expect = 4.167e-33 Identity = 88/224 (39.29%), Postives = 127/224 (56.70%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
++W+DIGG E +KT + E I++P++ G+ PKG+L +GPPGC KT+ ALATE + V +I VGESE A R I +A ++PS+IF ++ID L P R +S S +L+ L+ + G+E K V+IVA TN +D ALLRPGRL + +G PD R ILK F TE + ++ +A+ G SGAE+V + +E
Sbjct: 404 VYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAPKGVLLYGPPGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESERAIREIFRKARSAAPSIIFFDEIDALSPDRDGSSTS-AANHVLTSLLNEIDGVEELK--GVVIVAATNRPDEIDAALLRPGRLDRHIYVGPPDVNARLEILKK--CTKKFNTEESGV-DLHELADRTEGYSGAEVVLLCQE 621 HSP 3 Score: 140.584 bits (353), Expect = 2.123e-30 Identity = 85/240 (35.42%), Postives = 127/240 (52.92%), Query Frame = 3
Query: 1002 EPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQ-VRLGVRRALPLDT 1697
EPL + +GG + LK+ IE PL F +G+L HGPPG KTM +R +A + ++++I +I+ Y+GE+E LR IF+ A QPSI+FIDEID I P+R + V R+++ LL ++G+ V+ +AATN + +D A+ R GR +EIG+PD +R IL FS + D E IA + G GA++ R+ V ++R L D
Sbjct: 131 EPLSYAAVGGLDKEIESLKSAIEIPLHQPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTINGPSIVSKYLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAGKVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLD--SEAIKYIASKTHGYVGADLTALCRESVMKTIQRGLGTDA 368 HSP 4 Score: 132.88 bits (333), Expect = 5.173e-28 Identity = 98/341 (28.74%), Postives = 160/341 (46.92%), Query Frame = 3
Query: 2229 KLVPSQLDGF-----VNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTD-------------MRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISN-ISTIAESLTGLSGAEIVGIVRE 3173
K+ L G+ + +KV L D+ + MI++ L K+ E I+ ++ + + D+ + Y D I FS+E + K + +PL + +GG + L IE+P+ G+ P+GIL HGPPG KTM + +A + + I+ +GE+EAA R I ++A + PS+IFI++ID + P R+++ V R+++ L+T + G+ V+++A TN ++VD AL RPGR V IG+PD R IL FS ++ H + S I IA G GA++ + RE
Sbjct: 19 KVTVGSLQGYNILECMEEKVIQKLLDDSGVIMPGMIFQNLKTKAGDESIDVVITDASDDSLPDVSQLDLNMDDMYGGLDNLFYLSPPFIFRKGSTHITFSKETQANRKY--NLPEPLSYAAVGGLDKEIESLKSAIEIPLHQPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTINGPSIVSKYLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAG--KVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMS-SDRHVLDSEAIKYIASKTHGYVGADLTALCRE 354
BLAST of AAA family ATPase vs. TrEMBL
Match: R9T831 (AAA family ATPase, CDC48 subfamily protein OS=Methanomassiliicoccus intestinalis (strain Issoire-Mx1) OX=1295009 GN=MMINT_14960 PE=4 SV=1) HSP 1 Score: 184.882 bits (468), Expect = 1.027e-44 Identity = 141/458 (30.79%), Postives = 224/458 (48.91%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINCLTWFNE-----KDYLKIIYQTLIANKSIQMVLCCYFINSSNLNEE-------------FHLIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF-----IDNVYRKFKEITLE----------RLKACRKD-----SNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEI 1640
V++HGP G GK L K +A E N +F I ++ +++L+ I++ A ++ ++ I+S E ++D L ++ + PN+ RR +E+ + + DK R E+ + + GM D + KF+ N + + G DL+ + ++ R E+ LE ++ D PS + + + +HWVDIGG E K L+ +E PLK GK F KG+L +GPPG KTM +A+ATE+ N ISIK + +VGESE +R+ F +A P ++F+DEID I SR S V++R++S+LL L+G+ +V+ +AATN D +D A++R GR +EIGLPD SR+SIL I Q ADDV + +A+A++ G +GA++
Sbjct: 216 VLLHGPPGTGKTLLAKAVASET-NANFMTIGGPEIMSKYHGESEEHLREIFKQ--AQENAPTIIFIDEIDSIAPKREEVTGETERRVVAQMLSLMDGLESRGKVVVIGATNRPNALDPALRRPGRFDREIEIGVPDKAGRLEILQI---HTRGMPLEDGIDLDKFA-NLTHGYVGADLSALCKEAAMRSLRRILPELNLEDDIIPIEILNKITVSEDDFMGALMEMQPSSMREVLVERPNVHWVDIGGLEDAKRQLREAVEWPLKYGKVFRKMNAAPPKGILMYGPPGTGKTMLAKAVATESEANFISIKGPEFLSKWVGESEKAVRETFRKARQASPCVIFMDEIDSIATSRGSGDDSHVTERVISQLLTELDGLEGLQDVVVIAATNRPDMIDPALLRPGRFDRMVEIGLPDLESRKSILAI----QTANTPLADDV--DLTAVADKTEGYTGADL 660 HSP 2 Score: 160.614 bits (405), Expect = 1.048e-36 Identity = 86/224 (38.39%), Postives = 131/224 (58.48%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIKHG-------LEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
+HW DIGG E K L E +E P+K+G PKGIL +GPPG KTM A+ATE + ++ + + VGESE A R +A ++SP +IF+++ID + R S S+V ER++S+L+T L G+E Q+V+++A TN +D ALLRPGR +V IGLPD + R+SIL I ++ + +++ +A+ G +GA++ +V E
Sbjct: 449 VHWVDIGGLEDAKRQLREAVEWPLKYGKVFRKMNAAPPKGILMYGPPGTGKTMLAKAVATESEANFISIKGPEFLSKWVGESEKAVRETFRKARQASPCVIFMDEIDSIATSRGSGDDSHVTERVISQLLTELDGLEGL--QDVVVIAATNRPDMIDPALLRPGRFDRMVEIGLPDLESRKSILAIQTANTPLADD----VDLTAVADKTEGYTGADLSAVVNE 666 HSP 3 Score: 135.576 bits (340), Expect = 8.814e-29 Identity = 83/235 (35.32%), Postives = 125/235 (53.19%), Query Frame = 3
Query: 1008 LHWVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRALP 1688
+ + DIGG ++ IE PL++ G E KGVL HGPPG KT+ +A+A+ET N ++I IM Y GESE LR+IF +A P+I+FIDEID I P R+ + +R+++++L ++G+ + V+ + ATN + LD A+ R GR IEIG+PD+ R IL I G D + + A G GA++ ++ + +RR LP
Sbjct: 176 ISYEDIGGLGDEVKKVREMIELPLRHPELFERLGIEAPKGVLLHGPPGTGKTLLAKAVASETNANFMTIGGPEIMSKYHGESEEHLREIFKQAQENAPTIIFIDEIDSIAPKREEV-TGETERRVVAQMLSLMDGLESRGKVVVIGATNRPNALDPALRRPGRFDREIEIGVPDKAGRLEILQI----HTRGMPLEDGI--DLDKFANLTHGYVGADLSALCKEAAMRSLRRILP 403 HSP 4 Score: 133.65 bits (335), Expect = 4.450e-28 Identity = 84/283 (29.68%), Postives = 148/283 (52.30%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHNNNE-NVSDSLPTIKYQYFREAISASCSIGTMMELLKISCQEPSLDQLNIH 3347
+ + DIGG + E IE+P++H G+E PKG+L HGPPG KT+ A+A+E +G +IM GESE R I QA E++P++IFI++ID + PKR + R+++++++ + G+E++ V+++ TN + +D AL RPGR + IG+PD+ R IL+I + + ++ A G GA++ + +E ++ E N+ D + I+ + +S +G +ME+ S +E +++ N+H
Sbjct: 176 ISYEDIGGLGDEVKKVREMIELPLRHPELFERLGIEAPKGVLLHGPPGTGKTLLAKAVASETNANFMTIGGPEIMSKYHGESEEHLREIFKQAQENAPTIIFIDEIDSIAPKREEVT-GETERRVVAQMLSLMDGLESR--GKVVVIGATNRPNALDPALRRPGRFDREIEIGVPDKAGRLEILQIHTRGMPLEDG----IDLDKFANLTHGYVGADLSALCKEAAMRSLRRILPELNLEDDIIPIEI-LNKITVSEDDFMGALMEMQPSSMREVLVERPNVH 450
BLAST of AAA family ATPase vs. TrEMBL
Match: A7A1S2 (ATPase family protein OS=Saccharomyces cerevisiae (strain YJM789) OX=307796 GN=AFG2 PE=4 SV=1) HSP 1 Score: 185.267 bits (469), Expect = 1.870e-44 Identity = 139/458 (30.35%), Postives = 226/458 (49.34%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINCLTWFNEKDYLKIIYQTL--IANKSIQMVLCCYFINS-----------------SNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRRR----KEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF--------------IDNVYRKFK-EITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL---GITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIV 1643
+++HGP G GK L++ +A+ N IN + ++ YL L I N++ + FI+ S + ++D + ++ + PNS RR +EV + I D ++RF++ +R + + D E K+ ++ + + G DLT D KF ++TL+ +++ D PS + ++ ++W DIGG E +K +K I+ PL+ + F KGVL +GPPGCSKT+ +ALATE+G+N +++K I YVGESE +R+IF +A + PSI+F DEID + P RD + S + +++ LL ++G+ E V+ VAATN D++DAA++R GR+ HI +G PD +R IL F+ + G D+ E +A+R G SGAE+V
Sbjct: 282 ILLHGPPGTGKTMLLRVVAN-TSNAHVLTINGPSIVSK--YLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAGKVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSEAIKYIASKTHGYVGADLTALCRESVMKTIQRGLGTDANIDKFSLKVTLKDVESAMVDIR--PSAMREIFLEMPKVYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAPKGVLLYGPPGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESERAIREIFRKARSAAPSIIFFDEIDALSPDRDGSSTS-AANHVLTSLLNEIDGVEELKGVVIVAATNRPDEIDAALLRPGRLDRHIYVGPPDVNARLEILKKCTKKFNTEESGV----DLHE----LADRTEGYSGAEVV 725 HSP 2 Score: 148.288 bits (373), Expect = 1.262e-32 Identity = 88/224 (39.29%), Postives = 127/224 (56.70%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
++W+DIGG E +KT + E I++P++ G+ PKG+L +GPPGC KT+ ALATE + V +I VGESE A R I +A ++PS+IF ++ID L P R +S S +L+ L+ + G+E K V+IVA TN +D ALLRPGRL + +G PD R ILK F TE + ++ +A+ G SGAE+V + +E
Sbjct: 513 VYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAPKGVLLYGPPGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESERAIREIFRKARSAAPSIIFFDEIDALSPDRDGSSTS-AANHVLTSLLNEIDGVEELK--GVVIVAATNRPDEIDAALLRPGRLDRHIYVGPPDVNARLEILKK--CTKKFNTEESGV-DLHELADRTEGYSGAEVVLLCQE 730 HSP 3 Score: 140.969 bits (354), Expect = 2.906e-30 Identity = 85/240 (35.42%), Postives = 127/240 (52.92%), Query Frame = 3
Query: 1002 EPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQ-VRLGVRRALPLDT 1697
EPL + +GG + LK+ IE PL F +G+L HGPPG KTM +R +A + ++++I +I+ Y+GE+E LR IF+ A QPSI+FIDEID I P+R + V R+++ LL ++G+ V+ +AATN + +D A+ R GR +EIG+PD +R IL FS + D E IA + G GA++ R+ V ++R L D
Sbjct: 240 EPLSYAAVGGLDKEIESLKSAIEIPLHQPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTINGPSIVSKYLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAGKVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLD--SEAIKYIASKTHGYVGADLTALCRESVMKTIQRGLGTDA 477 HSP 4 Score: 132.494 bits (332), Expect = 1.181e-27 Identity = 96/340 (28.24%), Postives = 157/340 (46.18%), Query Frame = 3
Query: 2229 KLVPSQLDGF-----VNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTD-------------MRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
K+ L G+ + +KV L D+ + MI++ L K+ E I+ ++ + + D+ + Y D I FS+E + K + +PL + +GG + L IE+P+ G+ P+GIL HGPPG KTM + +A + + I+ +GE+EAA R I ++A + PS+IFI++ID + P R+++ V R+++ L+T + G+ V+++A TN ++VD AL RPGR V IG+PD R IL FS ++ I IA G GA++ + RE
Sbjct: 128 KVTVGSLQGYNILECMEEKVIQKLLDDSGVIMPGMIFQNLKTKAGDESIDVVITDASDDSLPDVSQLDLNMDDMYGGLDNLFYLSPPFIFRKGSTHITFSKETQANRKY--NLPEPLSYAAVGGLDKEIESLKSAIEIPLHQPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTINGPSIVSKYLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAG--KVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSEAIKYIASKTHGYVGADLTALCRE 463
BLAST of AAA family ATPase vs. TrEMBL
Match: N1NZT6 (Afg2p OS=Saccharomyces cerevisiae (strain CEN.PK113-7D) OX=889517 GN=CENPK1137D_768 PE=4 SV=1) HSP 1 Score: 184.882 bits (468), Expect = 1.939e-44 Identity = 139/458 (30.35%), Postives = 226/458 (49.34%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINCLTWFNEKDYLKIIYQTL--IANKSIQMVLCCYFINS-----------------SNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRRR----KEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF--------------IDNVYRKFK-EITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL---GITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIV 1643
+++HGP G GK L++ +A+ N IN + ++ YL L I N++ + FI+ S + ++D + ++ + PNS RR +EV + I D ++RF++ +R + + D E K+ ++ + + G DLT D KF ++TL+ +++ D PS + ++ ++W DIGG E +K +K I+ PL+ + F KGVL +GPPGCSKT+ +ALATE+G+N +++K I YVGESE +R+IF +A + PSI+F DEID + P RD + S + +++ LL ++G+ E V+ VAATN D++DAA++R GR+ HI +G PD +R IL F+ + G D+ E +A+R G SGAE+V
Sbjct: 282 ILLHGPPGTGKTMLLRVVAN-TSNAHVLTINGPSIVSK--YLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAGKVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSEAIKYIASKTHGYVGADLTALCRESVMKTIQRGLGTDANIDKFSLKVTLKDVESAMVDIR--PSAMREIFLEMPKVYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAPKGVLLYGPPGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESERAIREIFRKARSAAPSIIFFDEIDALSPDRDGSSTS-AANHVLTSLLNEIDGVEELKGVVIVAATNRPDEIDAALLRPGRLDRHIYVGPPDVNARLEILKKCTKKFNTEESGV----DLHE----LADRTEGYSGAEVV 725 HSP 2 Score: 148.288 bits (373), Expect = 1.378e-32 Identity = 88/224 (39.29%), Postives = 127/224 (56.70%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
++W+DIGG E +KT + E I++P++ G+ PKG+L +GPPGC KT+ ALATE + V +I VGESE A R I +A ++PS+IF ++ID L P R +S S +L+ L+ + G+E K V+IVA TN +D ALLRPGRL + +G PD R ILK F TE + ++ +A+ G SGAE+V + +E
Sbjct: 513 VYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAPKGVLLYGPPGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESERAIREIFRKARSAAPSIIFFDEIDALSPDRDGSSTS-AANHVLTSLLNEIDGVEELK--GVVIVAATNRPDEIDAALLRPGRLDRHIYVGPPDVNARLEILK--KCTKKFNTEESGV-DLHELADRTEGYSGAEVVLLCQE 730 HSP 3 Score: 140.584 bits (353), Expect = 3.144e-30 Identity = 85/240 (35.42%), Postives = 127/240 (52.92%), Query Frame = 3
Query: 1002 EPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQ-VRLGVRRALPLDT 1697
EPL + +GG + LK+ IE PL F +G+L HGPPG KTM +R +A + ++++I +I+ Y+GE+E LR IF+ A QPSI+FIDEID I P+R + V R+++ LL ++G+ V+ +AATN + +D A+ R GR +EIG+PD +R IL FS + D E IA + G GA++ R+ V ++R L D
Sbjct: 240 EPLSYAAVGGLDKEIESLKSAIEIPLHQPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTINGPSIVSKYLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAGKVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLD--SEAIKYIASKTHGYVGADLTALCRESVMKTIQRGLGTDA 477 HSP 4 Score: 132.494 bits (332), Expect = 1.255e-27 Identity = 96/340 (28.24%), Postives = 157/340 (46.18%), Query Frame = 3
Query: 2229 KLVPSQLDGF-----VNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTD-------------MRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
K+ L G+ + +KV L D+ + MI++ L K+ E I+ ++ + + D+ + Y D I FS+E + K + +PL + +GG + L IE+P+ G+ P+GIL HGPPG KTM + +A + + I+ +GE+EAA R I ++A + PS+IFI++ID + P R+++ V R+++ L+T + G+ V+++A TN ++VD AL RPGR V IG+PD R IL FS ++ I IA G GA++ + RE
Sbjct: 128 KVTVGSLQGYNILECMEEKVIQKLLDDSGVIMPGMIFQNLKTKAGDESIDVVITDASDDSLPDVSQLDLNMDDMYGGLDNLFYLSPPFIFRKGSTHITFSKETQANRKY--NLPEPLSYAAVGGLDKEIESLKSAIEIPLHQPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTINGPSIVSKYLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAG--KVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSEAIKYIASKTHGYVGADLTALCRE 463
BLAST of AAA family ATPase vs. Ensembl Cavefish
Match: zgc:136908 (zgc:136908 [Source:ZFIN;Acc:ZDB-GENE-060312-22]) HSP 1 Score: 145.206 bits (365), Expect = 4.243e-35 Identity = 133/485 (27.42%), Postives = 217/485 (44.74%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINSSNL----NEEFH------------LIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF-----IDNVYRKFKEITLE----------RLKACRKDSNWI-----PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR--DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENF-SAIAERLSGKSGAEIVEFGRQVRLGVRRALPLDTR 1700
++++GP G GK + + +A+E FF IN L +E + K + A K+ ++ FI+ + E+ H ++D L + + + PNS RR +E+ + I D R E+ + + M DD + ++ + + + G DL + + +K I LE L D W PS L + ++ ++W DIGG + VK L+ ++ P++ +F+K GVLF+GPPGC KT+ +A+A E N +SIK ++ + GESE +R +FD+A P ILF DE+D I SR + R+++++L ++G+ K NV + ATN D +D A++R GR+ I I LPD SR +IL A +V + S I E SG EI + R +L +R A+ + R
Sbjct: 184 ILLYGPPGTGKTLVARAVANET-GAFFFLINGPEIMSKLAGESESNLRKAFEE---AEKNAPAII---FIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRVHVVVMAATNRPNSVDPALRRFGRFDREIDIGIPDSTGRLEILQI---HTKNMKLADDVDLERIATDT-HGHVGADLAALCSEAALQAIRKKMTLIDLEDETIDADLLNSLAVTMDDFRWALSQSNPSALRETVVEVPQVNWEDIGGLDEVKRELQELVQYPVEYPDKFLKFGMTPSRGVLFYGPPGCGKTLLAKAIANECQANFVSIKGPELLTMWFGESEANVRDVFDKARQAAPCILFFDELDSIAKSRGGGAGDAGGAADRVINQILTEMDGMTNKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDMPSRTAILRANLRKSP----VAKEVDLPYLSRITEGFSGADLTEICQ--RACKLAIREAIEAEIR 651 HSP 2 Score: 135.191 bits (339), Expect = 6.092e-32 Identity = 97/351 (27.64%), Postives = 168/351 (47.86%), Query Frame = 3
Query: 2355 DIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSRE------IENTSKELD------HQSQP------------LHWTDIGGYETVKTMLIENIEMPI-------KHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGI--------VRE-------MKRKAQFHNNNENVSDSLPTIKYQYFREAI 3263
D+E + + HG D+ A+++ R M LI+ E + + + +D QS P ++W DIGG + VK L E ++ P+ K G+ +G+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R + +A +++P ++F +++D + R + +R++++++T + G+ KK NV I+ TN +D A+LRPGRL ++ I LPD R +IL+ E + + +S I E G SGA++ I +RE +R+ Q ++ D +P I+ +F EA+
Sbjct: 338 DLERIATDTHGHVGADLAALCSEAALQAIRKKM-TLIDLEDETIDADLLNSLAVTMDDFRWALSQSNPSALRETVVEVPQVNWEDIGGLDEVKRELQELVQYPVEYPDKFLKFGMTPSRGVLFYGPPGCGKTLLAKAIANECQANFVSIKGPELLTMWFGESEANVRDVFDKARQAAPCILFFDELDSIAKSRGGGAGDAGGAADRVINQILTEMDGMTNKK--NVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDMPSRTAILRANLRKSPVAKEVD-LPYLSRITE---GFSGADLTEICQRACKLAIREAIEAEIRAERERQSRKGTDDDYDPVPEIRKDHFEEAM 681 HSP 3 Score: 119.783 bits (299), Expect = 4.365e-27 Identity = 72/198 (36.36%), Postives = 107/198 (54.04%), Query Frame = 3
Query: 1101 KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRALPL 1691
+G+L +GPPG KT+ RA+A ETG I IM GESE LR+ F+ A P+I+FIDE+D I P R+ V +R++S+LL ++G+ ++ +V+ +AATN + +D A+ R GR I+IG+PD R IL I ADDV + IA G GA++ + L +R+ + L
Sbjct: 182 RGILLYGPPGTGKTLVARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTH-GEVERRIVSQLLTLMDGLKQRVHVVVMAATNRPNSVDPALRRFGRFDREIDIGIPDSTGRLEILQI----HTKNMKLADDV--DLERIATDTHGHVGADLAALCSEAALQAIRKKMTL 372 HSP 4 Score: 111.694 bits (278), Expect = 1.177e-24 Identity = 64/192 (33.33%), Postives = 107/192 (55.73%), Query Frame = 3
Query: 2613 PKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
P+GIL +GPPG KT+ A+A E F + +IM + GESE+ R +A +++P++IFI+++D + PKR + V RI+S+L+T + G+ K+ +V+++A TN ++VD AL R GR + IG+PD R IL+I ++ + ++ IA G GA++ + E +A
Sbjct: 181 PRGILLYGPPGTGKTLVARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKR-EKTHGEVERRIVSQLLTLMDGL--KQRVHVVVMAATNRPNSVDPALRRFGRFDREIDIGIPDSTGRLEILQIHTKNMKLADD----VDLERIATDTHGHVGADLAALCSEAALQA 365
BLAST of AAA family ATPase vs. Ensembl Cavefish
Match: nvl (nuclear VCP like [Source:NCBI gene;Acc:103033742]) HSP 1 Score: 134.035 bits (336), Expect = 2.287e-31 Identity = 81/238 (34.03%), Postives = 124/238 (52.10%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQV 1661
PS A + + W DIG + ++ L I P+++ ++F GVL GPPGC KT+ +A+A E+GLN IS+K ++ YVGESE +RQ+F R P ++F DEID + P R S S R++++LL ++G+ + V +AATN D +D AV+R GR+ + +GLP R +IL +T + DV A ER +GA++ R+
Sbjct: 624 PSAKREGFATVPDVTWDDIGALQDIREELTMAILAPVRSPEQFKALGLSAPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRGRNSAPCVIFFDEIDALCPRRSGHE-SGASVRVVNQLLTEMDGLETRRQVFIMAATNRPDIIDPAVLRPGRLDKTLYVGLPPPADRHAIL-LTITKGGMKPRLESDVSLEAIASDERCDCFTGADLSALVREA 859 HSP 2 Score: 132.109 bits (331), Expect = 9.803e-31 Identity = 76/223 (34.08%), Postives = 120/223 (53.81%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESI-LKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
W DIG + ++ L I P++ GL P G+L GPPGC KT+ A+A E L +V +++ VGESE A R + + S+P +IF ++ID L P+RS + +S R++++L+T + G+ET++ V I+A TN +D A+LRPGRL + +GLP DR +I L I + + E ++ E +GA++ +VRE
Sbjct: 639 WDDIGALQDIREELTMAILAPVRSPEQFKALGLSAPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRGRNSAPCVIFFDEIDALCPRRSGH-ESGASVRVVNQLLTEMDGLETRR--QVFIMAATNRPDIIDPAVLRPGRLDKTLYVGLPPPADRHAILLTITKGGMKPRLESDVSLEAIASDERCDCFTGADLSALVRE 858 HSP 3 Score: 122.094 bits (305), Expect = 1.159e-27 Identity = 74/247 (29.96%), Postives = 129/247 (52.23%), Query Frame = 3
Query: 2469 FSREIENTSKELDHQSQPLHWTDIGGYETVKTML------IENIEMPIKHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIET-KKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
S++ + EL H + + + D+GG E T L + + E+ + G+ P+G L HGPPGC KT+ A+A E +L M V +++ G+ GESE R + QA+ ++P ++FI++ID + PKR SK ++ RI+++L+T + + + V+++ TN ++D AL R GR + +G+PD+ R IL+ L + + +A G GA+++ + RE A
Sbjct: 257 MSKKARTKAPELQHST--VKFEDVGGNEETLTELCKLLIHMRHPEVYQQLGVVPPRGFLLHGPPGCGKTLLAQAVAGEMELPMLKVSAPELVSGVSGESEQKLRELFDQAVSNAPCILFIDEIDAITPKREVASK-DMERRIVAQLLTCMDDLNSLSVPAQVLVIGATNRPDSLDPALRRAGRFDREICLGIPDEGTRLRILQTLCRKLRLPVDF----DFRQLARLTPGYVGADLMALCREAAMSA 496 HSP 4 Score: 117.857 bits (294), Expect = 2.336e-26 Identity = 60/147 (40.82%), Postives = 92/147 (62.59%), Query Frame = 3
Query: 1101 KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINE---KPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
+G L HGPPGC KT+ +A+A E L ++ + ++ G GESE LR++FD+AV+ P ILFIDEID I P R+ +A + +R++++LL ++ +N V+ + ATN D LD A+ R+GR I +G+PD+ +R IL
Sbjct: 310 RGFLLHGPPGCGKTLLAQAVAGEMELPMLKVSAPELVSGVSGESEQKLRELFDQAVSNAPCILFIDEIDAITPKRE-VASKDMERRIVAQLLTCMDDLNSLSVPAQVLVIGATNRPDSLDPALRRAGRFDREICLGIPDEGTRLRIL 455
BLAST of AAA family ATPase vs. Ensembl Cavefish
Match: nvl (nuclear VCP like [Source:NCBI gene;Acc:103033742]) HSP 1 Score: 134.035 bits (336), Expect = 2.287e-31 Identity = 81/238 (34.03%), Postives = 124/238 (52.10%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQV 1661
PS A + + W DIG + ++ L I P+++ ++F GVL GPPGC KT+ +A+A E+GLN IS+K ++ YVGESE +RQ+F R P ++F DEID + P R S S R++++LL ++G+ + V +AATN D +D AV+R GR+ + +GLP R +IL +T + DV A ER +GA++ R+
Sbjct: 624 PSAKREGFATVPDVTWDDIGALQDIREELTMAILAPVRSPEQFKALGLSAPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRGRNSAPCVIFFDEIDALCPRRSGHE-SGASVRVVNQLLTEMDGLETRRQVFIMAATNRPDIIDPAVLRPGRLDKTLYVGLPPPADRHAIL-LTITKGGMKPRLESDVSLEAIASDERCDCFTGADLSALVREA 859 HSP 2 Score: 132.109 bits (331), Expect = 9.803e-31 Identity = 76/223 (34.08%), Postives = 120/223 (53.81%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESI-LKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
W DIG + ++ L I P++ GL P G+L GPPGC KT+ A+A E L +V +++ VGESE A R + + S+P +IF ++ID L P+RS + +S R++++L+T + G+ET++ V I+A TN +D A+LRPGRL + +GLP DR +I L I + + E ++ E +GA++ +VRE
Sbjct: 639 WDDIGALQDIREELTMAILAPVRSPEQFKALGLSAPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRGRNSAPCVIFFDEIDALCPRRSGH-ESGASVRVVNQLLTEMDGLETRR--QVFIMAATNRPDIIDPAVLRPGRLDKTLYVGLPPPADRHAILLTITKGGMKPRLESDVSLEAIASDERCDCFTGADLSALVRE 858 HSP 3 Score: 122.094 bits (305), Expect = 1.159e-27 Identity = 74/247 (29.96%), Postives = 129/247 (52.23%), Query Frame = 3
Query: 2469 FSREIENTSKELDHQSQPLHWTDIGGYETVKTML------IENIEMPIKHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIET-KKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
S++ + EL H + + + D+GG E T L + + E+ + G+ P+G L HGPPGC KT+ A+A E +L M V +++ G+ GESE R + QA+ ++P ++FI++ID + PKR SK ++ RI+++L+T + + + V+++ TN ++D AL R GR + +G+PD+ R IL+ L + + +A G GA+++ + RE A
Sbjct: 257 MSKKARTKAPELQHST--VKFEDVGGNEETLTELCKLLIHMRHPEVYQQLGVVPPRGFLLHGPPGCGKTLLAQAVAGEMELPMLKVSAPELVSGVSGESEQKLRELFDQAVSNAPCILFIDEIDAITPKREVASK-DMERRIVAQLLTCMDDLNSLSVPAQVLVIGATNRPDSLDPALRRAGRFDREICLGIPDEGTRLRILQTLCRKLRLPVDF----DFRQLARLTPGYVGADLMALCREAAMSA 496 HSP 4 Score: 117.857 bits (294), Expect = 2.336e-26 Identity = 60/147 (40.82%), Postives = 92/147 (62.59%), Query Frame = 3
Query: 1101 KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINE---KPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
+G L HGPPGC KT+ +A+A E L ++ + ++ G GESE LR++FD+AV+ P ILFIDEID I P R+ +A + +R++++LL ++ +N V+ + ATN D LD A+ R+GR I +G+PD+ +R IL
Sbjct: 310 RGFLLHGPPGCGKTLLAQAVAGEMELPMLKVSAPELVSGVSGESEQKLRELFDQAVSNAPCILFIDEIDAITPKRE-VASKDMERRIVAQLLTCMDDLNSLSVPAQVLVIGATNRPDSLDPALRRAGRFDREICLGIPDEGTRLRIL 455
BLAST of AAA family ATPase vs. Ensembl Cavefish
Match: SPATA5 (spermatogenesis associated 5 [Source:HGNC Symbol;Acc:HGNC:18119]) HSP 1 Score: 132.494 bits (332), Expect = 6.240e-31 Identity = 99/338 (29.29%), Postives = 155/338 (45.86%), Query Frame = 3
Query: 2346 SVEDIEELVKNLHGFTPKDIRWFLKSAS----------------------VKYSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA-QFHNNNENVSDSLPTIKYQYFREAISA 3269
S E+++EL HG+ D+ K A VK + +D+R ++ + + +E+ + W+D+GG E VK L + +E P+KH G+ PKG+L +GPPGC KTM ALA E QL + +++ VGESE A R + +A +PS++F ++ID L +R + ++ + L+ + K NV N LH AL+RPGRL IV + LPD R I + F + + + ++ T E SGAEI + RE A Q E+++D ++F A+SA
Sbjct: 519 STEELQELADAAHGYVGADLAAVCKEAGLHALRRVLGSAPGLCDPEVMDRVKVTASDLRLAMKHVKP--SAMREVAIDVPKVRWSDVGGMEDVKLKLKQAVEWPLKHPEAFARLGIAPPKGVLLYGPPGCSKTMIAKALANESQLNFLAIKGPELLSKYVGESERAVREVFRKARTVAPSIVFFDEIDALAVER---GRYDISHSFIHSLL-----FQNKPVSNV------NTLHTNRPALMRPGRLDRIVYVPLPDSATRREIFSLQFRSMPVHSSVH-LDDLVTRTER---YSGAEITAVCREAALFALQEDIKAEHITD-------RHFDSALSA 829 HSP 2 Score: 129.413 bits (324), Expect = 4.797e-30 Identity = 85/241 (35.27%), Postives = 119/241 (49.38%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQN-DGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
PS + D+ + W D+GG E VK+ LK +E PLK+ + F KGVL +GPPGCSKTM +ALA E+ LN ++IK ++ YVGESE +R++F +A + PSI+F DEID + R +S L+F N KP V+ N A+MR GR+ + + LPD +R I + F + DD + R SGAEI R+ L
Sbjct: 585 PSAMREVAIDVPKVRWSDVGGMEDVKLKLKQAVEWPLKHPEAFARLGIAPPKGVLLYGPPGCSKTMIAKALANESQLNFLAIKGPELLSKYVGESERAVREVFRKARTVAPSIVFFDEIDALAVERGRYDIS----HSFIHSLLFQN----KP----VSNVNTLHTNRPALMRPGRLDRIVYVPLPDSATRREIFSLQFRSMPVHSSVHLDD-------LVTRTERYSGAEITAVCREAAL 806 HSP 3 Score: 127.872 bits (320), Expect = 1.376e-29 Identity = 71/245 (28.98%), Postives = 131/245 (53.47%), Query Frame = 3
Query: 2463 IEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQ-NVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
+ F+ T+++ + + ++ IGG + E IE+P+KH G+ P+G+L +GPPG KTM A+A E M + +IM GE+EA R I ++A + P++IFI+++D L PKR +++ V +R+++ L+T + GI ++ + ++++ TN H +D AL RPGR + +G+P + R IL+ + + E + +A++ G GA++ + +E
Sbjct: 304 LSFTHSQPQTTEDQGQERSKVTYSMIGGLSAQLAAIRETIELPLKHPEIFRKYGIPPPRGVLLYGPPGTGKTMIGRAVANEVGAHMTVINGPEIMSKFYGETEARLRQIFAEASQKQPAIIFIDELDALCPKR-EGAQNEVEKRVVASLLTLMDGIGSEGHSGQLLVLGATNRPHALDPALRRPGRFDKELEVGVPAAEGRMDILQKQLRSVPCEISTE---ELQELADAAHGYVGADLAAVCKE 544 HSP 4 Score: 117.857 bits (294), Expect = 1.965e-26 Identity = 78/232 (33.62%), Postives = 119/232 (51.29%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPN---VIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRAL 1685
IGG + ++ IE PLK+ + F K GVL +GPPG KTM RA+A E G ++ I IM + GE+E LRQIF A QP+I+FIDE+D + P R+ A + V +R+++ LL ++GI + + ++ + ATN LD A+ R GR +E+G+P R IL + + E +A+ G GA++ ++ L +RR L
Sbjct: 329 IGGLSAQLAAIRETIELPLKHPEIFRKYGIPPPRGVLLYGPPGTGKTMIGRAVANEVGAHMTVINGPEIMSKFYGETEARLRQIFAEASQKQPAIIFIDELDALCPKREG-AQNEVEKRVVASLLTLMDGIGSEGHSGQLLVLGATNRPHALDPALRRPGRFDKELEVGVPAAEGRMDILQKQLRS-----VPCEISTEELQELADAAHGYVGADLAAVCKEAGLHALRRVL 554
BLAST of AAA family ATPase vs. Ensembl Cavefish
Match: katna1 (katanin catalytic subunit A1 [Source:NCBI gene;Acc:103037638]) HSP 1 Score: 125.561 bits (314), Expect = 7.816e-30 Identity = 79/241 (32.78%), Postives = 125/241 (51.87%), Query Frame = 3
Query: 2481 IENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIK-----HGLEIP-KGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGI----ETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
IE +++ Q+ + W DI E K +L E + +P+ G+ P KG+L GPPG KT+ A+ATEC+ FNV S + GESE R + A +P+ IFI++ID + +R ++ + R+ +EL+ + G+ E ++ VM++A TN ++DEAL R RL + I LP K R +LKI +L + ++ IAE + G SGA+I + R+
Sbjct: 185 IETLERDIISQNPNVKWDDIADLEEAKKLLKEAVVLPMWMPEFFKGIRRPWKGVLMVGPPGTGKTLLAKAVATECRTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSMCSRRGTSEEHEASRRVKAELLVQMDGVGGTSENDPSKMVMVLAATNFPWDIDEALRR--RLEKRIYIPLPSAKGRVDLLKINLKELELAND----VDMDKIAEQMEGYSGADITNVCRD 419 HSP 2 Score: 111.309 bits (277), Expect = 4.826e-25 Identity = 81/239 (33.89%), Postives = 120/239 (50.21%), Query Frame = 3
Query: 1008 LHWVDIGGYESVKVMLKNEIEEPL------KNGKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGI-----NEKPNVIFV-AATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRAL 1685
+ W DI E K +LK + P+ K + KGVL GPPG KT+ +A+ATE ++ ST+ Y GESE L+R +F+ A P+ +FIDEID + R S+R+ +ELL+ ++G+ N+ ++ V AATNF +D A+ R R+ I I LP R +L I + A+DV + IAE++ G SGA+I R L +RR +
Sbjct: 199 VKWDDIADLEEAKKLLKEAVVLPMWMPEFFKGIRRPWKGVLMVGPPGTGKTLLAKAVATECRTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSMCSRRGTSEEHEASRRVKAELLVQMDGVGGTSENDPSKMVMVLAATNFPWDIDEALRR--RLEKRIYIPLPSAKGRVDLLKINLKE----LELANDV--DMDKIAEQMEGYSGADITNVCRDASLMAMRRRI 429
BLAST of AAA family ATPase vs. Ensembl Sea Lamprey
Match: vcp (valosin containing protein [Source:NCBI gene;Acc:327197]) HSP 1 Score: 143.665 bits (361), Expect = 1.008e-37 Identity = 99/312 (31.73%), Postives = 155/312 (49.68%), Query Frame = 3
Query: 696 PNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF-----IDNVYRKFKEITLE----------RLKACRKDSNWI-----PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR--DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
PNS RR +EV + I D R E+ + + M D + ++ N+ + G DL + + +K I LE L D W PS L + ++ + W DIGG ESVK L+ ++ P+++ ++F+K GVLF+GPPGC KT+ +A+A E N ISIK ++ + GESE +R +FD+A P +LF DE+D I +R + + R+++++L ++G+ K NV + ATN D +D A++R GR+ I I LPD+ SR +IL
Sbjct: 7 PNSIDPALRRFGRFDREVDIGIPDSTGRLEILQI---HTKNMKLSSDVDLEQVG-NETHGHVGADLAALCSEAALQAIRKKMDVIDLEDETIDAEIMNSLAVTMDDFRWALGQSNPSALRETVVEVPNVTWEDIGGLESVKRELQELVQYPVEHPEKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDVFDKARQAAPCVLFFDELDSIAKARGGNVGDGGGAADRVINQILTEMDGMGTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRSAIL 314 HSP 2 Score: 139.043 bits (349), Expect = 3.625e-36 Identity = 89/287 (31.01%), Postives = 147/287 (51.22%), Query Frame = 3
Query: 2223 DLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILK 3056
DL+ V ++ G V + +E L+ + K+ I E I+ + N T D RW L +++ L E E+ N + W DIGG E+VK L E ++ P++H G+ KG+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R + +A +++P ++F +++D + R + +R++++++T + G+ TKK NV I+ TN +D A+LRPGRL ++ I LPD+K R +ILK
Sbjct: 52 DLEQVGNETHGHVGADLAALC---SEAALQAIRKKMDVIDLEDETIDAEIMNSLAVTMDDFRWAL-------GQSNPSALRETVVEVPNVT-----------WEDIGGLESVKRELQELVQYPVEHPEKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDVFDKARQAAPCVLFFDELDSIAKARGGNVGDGGGAADRVINQILTEMDGMGTKK--NVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRSAILK 315
BLAST of AAA family ATPase vs. Ensembl Sea Lamprey
Match: vcp (valosin containing protein [Source:NCBI gene;Acc:327197]) HSP 1 Score: 144.821 bits (364), Expect = 3.579e-37 Identity = 88/265 (33.21%), Postives = 148/265 (55.85%), Query Frame = 3
Query: 921 ITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR--DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEF-GRQVRLGVRRAL 1685
+T++ + SN PS L + ++ + W DIGG ESVK L+ ++ P+++ ++F+K GVLF+GPPGC KT+ +A+A E N ISIK ++ + GESE +R +FD+A P++LF+DE+D I +R + R+++++L ++G+ NV + ATN D +D A++R GR+ I I LPD+ SR +IL A DV + +++ +G SGA+++E R +L +R ++
Sbjct: 87 VTMDDFRWALAQSN--PSALREMVVEVPNVTWEDIGGLESVKRELQELVQYPVEHPEKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDVFDKARQASPTVLFLDELDSIAKARGGGVGDGGGAADRVINQILTEMDGMGVNKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRTAILKANLRKSP----IAKDV--DIGYLSKTTNGFSGADLMEICQRACKLAIRESI 343 HSP 2 Score: 144.05 bits (362), Expect = 6.208e-37 Identity = 94/325 (28.92%), Postives = 164/325 (50.46%), Query Frame = 3
Query: 2223 DLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVR 3170
DL+ + +Q G V + +E L+ + K+ I E I+ + N T D RW L ++++ L E E+ N + W DIGG E+VK L E ++ P++H G+ KG+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R + +A ++SP+++F++++D + R +R++++++T + G+ N+NV I+ TN +D A+LRPGRL ++ I LPD+K R +ILK + +I ++++ G SGA+++ I +
Sbjct: 35 DLEQIANQTHGHVGADLAALC---SEAALQAIRKKMDVIDLDDETIDAEIMNSLAVTMDDFRWAL-------AQSNPSALREMVVEVPNVT-----------WEDIGGLESVKRELQELVQYPVEHPEKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDVFDKARQASPTVLFLDELDSIAKARGGGVGDGGGAADRVINQILTEMDGMGV--NKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRTAILKANLRKSPIAKD----VDIGYLSKTTNGFSGADLMEICQ 332
BLAST of AAA family ATPase vs. Ensembl Sea Lamprey
Match: nvl (nuclear VCP like [Source:ZFIN;Acc:ZDB-GENE-040426-2871]) HSP 1 Score: 145.591 bits (366), Expect = 8.343e-37 Identity = 91/245 (37.14%), Postives = 130/245 (53.06%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR-DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRR 1679
PS A + + W DIG E+++ L I P+++ + F G+L GPPGC KTM +A+A E+GLN IS+K ++ YVGESE +RQ+F RA P ++F DEID I P R DH S S R++++LL ++G+ + V +AATN D LD AV+R GR+ + +GLP R +IL T + DV N A R G SGA++ R+ +G R
Sbjct: 242 PSAKREGFATVPDVTWDDIGALEAIREELSLAILAPIRHPEMFQVLGLTSPAGILLAGPPGCGKTMLAKAIANESGLNFISVKGPELLNMYVGESERAVRQVFQRARNSAPCVIFFDEIDAICPRRSDH--ESGASVRVVNQLLTEMDGLEARKQVFIMAATNRPDILDRAVLRPGRLDKTLYVGLPVMAERAAILR-TLTRGGTKPPLEKDVDLNVIAGDGRSDGFSGADLQALTREAAIGALR 483 HSP 2 Score: 135.191 bits (339), Expect = 2.050e-33 Identity = 80/223 (35.87%), Postives = 121/223 (54.26%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILK-IGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
W DIG E ++ L I PI+H GL P GIL GPPGC KTM A+A E L +V +++ VGESE A R + +A S+P +IF ++ID + P+R S+ +S R++++L+T + G+E +K V I+A TN +D A+LRPGRL + +GLP +R +IL+ + E ++ N+ G SGA++ + RE
Sbjct: 257 WDDIGALEAIREELSLAILAPIRHPEMFQVLGLTSPAGILLAGPPGCGKTMLAKAIANESGLNFISVKGPELLNMYVGESERAVRQVFQRARNSAPCVIFFDEIDAICPRR-SDHESGASVRVVNQLLTEMDGLEARK--QVFIMAATNRPDILDRAVLRPGRLDKTLYVGLPVMAERAAILRTLTRGGTKPPLEKDVDLNVIAGDGRSDGFSGADLQALTRE 476 HSP 3 Score: 88.5817 bits (218), Expect = 2.262e-18 Identity = 58/174 (33.33%), Postives = 94/174 (54.02%), Query Frame = 3
Query: 1167 ETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRAL 1685
E + LI + ++ G GESE LR++F A P +LF+DEID I P R+ +A + +R++++LL ++G++ V+ + ATN D LD A+ R+GR I +G+PD+ +RE IL + G ++ NF +A G GA+++ R+ V RAL
Sbjct: 1 ELDVPLIQVAGPEVVSGVSGESEQKLRELFQVASESAPCVLFLDEIDAITPKRE-MASKDMERRLVAQLLTCMDGLHGGGAVLCIGATNRPDALDPALRRAGRFDREICLGIPDENARERILLVLCR----GLRLKEEF--NFKRLARLTPGYVGADLMSLCREAAASAVNRAL 167 HSP 4 Score: 82.0333 bits (201), Expect = 2.837e-16 Identity = 54/184 (29.35%), Postives = 92/184 (50.00%), Query Frame = 3
Query: 2682 ECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHNNNENVSDSLPT 3233
E + + V +++ G+ GESE R + A ES+P ++F+++ID + PKR SK ++ R++++L+T + G+ V+ + TN +D AL R GR + +G+PD+ RE IL + L + E N +A G GA+++ + RE A N + D PT
Sbjct: 1 ELDVPLIQVAGPEVVSGVSGESEQKLRELFQVASESAPCVLFLDEIDAITPKREMASK-DMERRLVAQLLTCMDGLHG--GGAVLCIGATNRPDALDPALRRAGRFDREICLGIPDENARERILLVLCRGLRLKEEF----NFKRLARLTPGYVGADLMSLCREA--AASAVNRALHAIDVAPT 175
BLAST of AAA family ATPase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006168.1 (pep scaffold:Pmarinus_7.0:GL486395:211235:217029:-1 gene:ENSPMAG00000005566.1 transcript:ENSPMAT00000006168.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 115.931 bits (289), Expect = 2.397e-28 Identity = 70/205 (34.15%), Postives = 107/205 (52.20%), Query Frame = 3
Query: 972 SELLAYIADLEP-LHWVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR--DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQN 1556
S + AD +P + +VDIGG + K ++ +E PL + G + +GVL +GPPGC KTM +A+A T I + S ++ Y+GE ++R +F A P+I+FIDEID I R QR++ ELL ++G ++ NV + ATN D LD A++R GR+ IE +PD+ + I + N
Sbjct: 104 SSITMLTADQKPDVTYVDIGGMDIQKQEVREAVELPLTHFDLYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQTVNVKVIMATNRADTLDPALLRPGRLDRKIEFPMPDRRQKRLIFSTVTAKMN 308 HSP 2 Score: 114.775 bits (286), Expect = 5.103e-28 Identity = 63/202 (31.19%), Postives = 104/202 (51.49%), Query Frame = 3
Query: 2511 QSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTE 3089
Q + + DIGG + K + E +E+P+ H G++ P+G+L +GPPGC KTM A+A V S+ ++ +GE R + A E++P++IFI++ID + KR + +RIL EL+ + G + + NV ++ TN +D ALLRPGRL + +PD++ + I + +N E
Sbjct: 113 QKPDVTYVDIGGMDIQKQEVREAVELPLTHFDLYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFD--QTVNVKVIMATNRADTLDPALLRPGRLDRKIEFPMPDRRQKRLIFSTVTAKMNLSEE 312
BLAST of AAA family ATPase vs. Ensembl Sea Lamprey
Match: psmc5 (proteasome 26S subunit, ATPase 5 [Source:ZFIN;Acc:ZDB-GENE-030131-6547]) HSP 1 Score: 116.316 bits (290), Expect = 7.159e-28 Identity = 75/222 (33.78%), Postives = 115/222 (51.80%), Query Frame = 3
Query: 2538 IGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR---SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
IGG + + E IE+P+KH G+ PKG+L +GPPG KT+ A+A + V S++++ +GE R + A E +PS+IF+++ID + R S S V +R + EL+ L G E K N+ ++ TN + +D ALLRPGR+ + P+++ R ILKI +N N+ IAE + G SGAE+ G+ E
Sbjct: 155 IGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTECTFIRVSGSELVQKFIGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEV-QRTMLELLNQLDGFEATK--NIKVIMATNRIDILDPALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRG----INLRKIAELMPGASGAEVKGVCTE 369 HSP 2 Score: 110.153 bits (274), Expect = 8.110e-26 Identity = 78/215 (36.28%), Postives = 110/215 (51.16%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVS--QRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEI 1640
IGG + +K IE P+K+ + F KGVL +GPPG KT+ RA+A T I + S +++ ++GE ++R++F A PSI+F+DEID I SR S QR M ELL L+G N+ + ATN D LD A++R GR+ IE P++ +R IL I N + N IAE + G SGAE+
Sbjct: 155 IGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTECTFIRVSGSELVQKFIGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDPALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMN----LTRGI--NLRKIAELMPGASGAEV 363
BLAST of AAA family ATPase vs. Ensembl Yeast
Match: AFG2 (ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; target of the ribosomal biosynthesis inhibitor diazaborine; may be involved in degradation of aberrant mRNAs [Source:SGD;Acc:S000004389]) HSP 1 Score: 184.882 bits (468), Expect = 9.421e-49 Identity = 139/458 (30.35%), Postives = 226/458 (49.34%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINCLTWFNEKDYLKIIYQTL--IANKSIQMVLCCYFINS-----------------SNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRRR----KEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF--------------IDNVYRKFK-EITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL---GITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIV 1643
+++HGP G GK L++ +A+ N IN + ++ YL L I N++ + FI+ S + ++D + ++ + PNS RR +EV + I D ++RF++ +R + + D E K+ ++ + + G DLT D KF ++TL+ +++ D PS + ++ ++W DIGG E +K +K I+ PL+ + F KGVL +GPPGCSKT+ +ALATE+G+N +++K I YVGESE +R+IF +A + PSI+F DEID + P RD + S + +++ LL ++G+ E V+ VAATN D++DAA++R GR+ HI +G PD +R IL F+ + G D+ E +A+R G SGAE+V
Sbjct: 282 ILLHGPPGTGKTMLLRVVAN-TSNAHVLTINGPSIVSK--YLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAGKVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSEAIKYIASKTHGYVGADLTALCRESVMKTIQRGLGTDANIDKFSLKVTLKDVESAMVDIR--PSAMREIFLEMPKVYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAPKGVLLYGPPGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESERAIREIFRKARSAAPSIIFFDEIDALSPDRDGSSTS-AANHVLTSLLNEIDGVEELKGVVIVAATNRPDEIDAALLRPGRLDRHIYVGPPDVNARLEILKKCTKKFNTEESGV----DLHE----LADRTEGYSGAEVV 725 HSP 2 Score: 148.288 bits (373), Expect = 6.695e-37 Identity = 88/224 (39.29%), Postives = 127/224 (56.70%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
++W+DIGG E +KT + E I++P++ G+ PKG+L +GPPGC KT+ ALATE + V +I VGESE A R I +A ++PS+IF ++ID L P R +S S +L+ L+ + G+E K V+IVA TN +D ALLRPGRL + +G PD R ILK F TE + ++ +A+ G SGAE+V + +E
Sbjct: 513 VYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAPKGVLLYGPPGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESERAIREIFRKARSAAPSIIFFDEIDALSPDRDGSSTS-AANHVLTSLLNEIDGVEELK--GVVIVAATNRPDEIDAALLRPGRLDRHIYVGPPDVNARLEILK--KCTKKFNTEESGV-DLHELADRTEGYSGAEVVLLCQE 730 HSP 3 Score: 140.584 bits (353), Expect = 1.528e-34 Identity = 85/240 (35.42%), Postives = 127/240 (52.92%), Query Frame = 3
Query: 1002 EPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQ-VRLGVRRALPLDT 1697
EPL + +GG + LK+ IE PL F +G+L HGPPG KTM +R +A + ++++I +I+ Y+GE+E LR IF+ A QPSI+FIDEID I P+R + V R+++ LL ++G+ V+ +AATN + +D A+ R GR +EIG+PD +R IL FS + D E IA + G GA++ R+ V ++R L D
Sbjct: 240 EPLSYAAVGGLDKEIESLKSAIEIPLHQPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTINGPSIVSKYLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAGKVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLD--SEAIKYIASKTHGYVGADLTALCRESVMKTIQRGLGTDA 477 HSP 4 Score: 132.494 bits (332), Expect = 6.098e-32 Identity = 96/340 (28.24%), Postives = 157/340 (46.18%), Query Frame = 3
Query: 2229 KLVPSQLDGF-----VNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTD-------------MRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
K+ L G+ + +KV L D+ + MI++ L K+ E I+ ++ + + D+ + Y D I FS+E + K + +PL + +GG + L IE+P+ G+ P+GIL HGPPG KTM + +A + + I+ +GE+EAA R I ++A + PS+IFI++ID + P R+++ V R+++ L+T + G+ V+++A TN ++VD AL RPGR V IG+PD R IL FS ++ I IA G GA++ + RE
Sbjct: 128 KVTVGSLQGYNILECMEEKVIQKLLDDSGVIMPGMIFQNLKTKAGDESIDVVITDASDDSLPDVSQLDLNMDDMYGGLDNLFYLSPPFIFRKGSTHITFSKETQANRKY--NLPEPLSYAAVGGLDKEIESLKSAIEIPLHQPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTINGPSIVSKYLGETEAALRDIFNEARKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAG--KVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSEAIKYIASKTHGYVGADLTALCRE 463
BLAST of AAA family ATPase vs. Ensembl Yeast
Match: CDC48 (AAA ATPase; subunit of polyUb-selective segregase complex involved in ERAD, INM-associated degradation (INMAD), mitotic spindle disassembly, macroautophagy, PMN, ribosome-associated degradation, ribophagy, homotypic ER membrane fusion, SCF complex disassembly, cell wall integrity during heat stress, and telomerase regulation; mobilizes membrane-anchored transcription factors by regulated Ub/proteasome-dependent processing (RUP); human ortholog VCP complements a cdc48 mutant [Source:SGD;Acc:S000002284]) HSP 1 Score: 148.288 bits (373), Expect = 7.993e-37 Identity = 127/466 (27.25%), Postives = 211/466 (45.28%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-----SNLNEEFH--------LIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLT----------------------DFIDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHL--AVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQ--NDGFIFADDVKENFSAIAERLSGKSGAEIV 1643
V+M+GP G GK + + +A+E FF IN + +E + K + A K+ ++ I+S N E ++D + S + + PNS RR +EV + I D R E+ + + M DD + + + + + + G D+ D ID +T++ + +SN PS L + + + W D+GG + +K LK +E P+ + ++ K GVLF+GPPG KT+ +A+ATE N IS+K ++ + GESE +R IFD+A A P+++F+DE+D I +R S R++++LL ++G+N K NV + ATN D++D A++R GR+ I + LPD+ +R SIL G +AIA+ G SGA+++
Sbjct: 251 VLMYGPPGTGKTLMARAVANET-GAFFFLINGPEVMSKMAGESESNLRKAFEE---AEKNAPAIIFIDEIDSIAPKRDKTNGEVERRVVSQLLTLMDGMKARSNVVVIAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEVLRI---HTKNMKLADDVDLEALA-AETHGYVGADIASLCSEAAMQQIREKMDLIDLDEDEIDAEVLDSLGVTMDNFRFALGNSN--PSALRETVVESVNVTWDDVGGLDEIKEELKETVEYPVLHPDQYTKFGLSPSKGVLFYGPPGTGKTLLAKAVATEVSANFISVKGPELLSMWYGESESNIRDIFDKARAAAPTVVFLDELDSIAKARGGSLGDAGGASDRVVNQLLTEMDGMNAKKNVFVIGATNRPDQIDPAILRPGRLDQLIYVPLPDENARLSILNAQLRKTPLEPGL--------ELTAIAKATQGFSGADLL 698 HSP 2 Score: 138.658 bits (348), Expect = 8.560e-34 Identity = 108/395 (27.34%), Postives = 187/395 (47.34%), Query Frame = 3
Query: 2253 GFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVE-DIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDH-------------QSQP------------LHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHNNNENVSDSLPTIKYQYFREAISASCSIGTMMELLKISCQEPSLD 3332
G +++V + +P D L+ + N+K + + D+E L HG+ DI A+++ R M LI+ E E ++ LD S P + W D+GG + +K L E +E P+ H GL KG+LF+GPPG KT+ A+ATE +V +++ GESE+ R I +A ++P+++F++++D + R S +R++++L+T + G+ KK NV ++ TN +D A+LRPGRL ++ + LPD+ R SIL +T E ++ IA++ G SGA+++ IV +R A++ + DS+ + + + M + + QEP +D
Sbjct: 371 GRFDREVDIGIP-DATGRLEVLRIHTKNMKLADDVDLEALAAETHGYVGADIASLCSEAAMQQIREKM-DLIDLD-EDEIDAEVLDSLGVTMDNFRFALGNSNPSALRETVVESVNVTWDDVGGLDEIKEELKETVEYPVLHPDQYTKFGLSPSKGVLFYGPPGTGKTLLAKAVATEVSANFISVKGPELLSMWYGESESNIRDIFDKARAAAPTVVFLDELDSIAKARGGSLGDAGGASDRVVNQLLTEMDGMNAKK--NVFVIGATNRPDQIDPAILRPGRLDQLIYVPLPDENARLSILNAQLR----KTPLEPGLELTAIAKATQGFSGADLLYIV---QRAAKYA-----IKDSIEAHRQHEAEKEVKVEGEDVEMTDEGAKAEQEPEVD 748 HSP 3 Score: 132.109 bits (331), Expect = 9.591e-32 Identity = 79/217 (36.41%), Postives = 115/217 (53.00%), Query Frame = 3
Query: 1014 WVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIV 1643
+ DIGG ++ +E PL++ + F +GVL +GPPG KT+ RA+A ETG I +M GESE LR+ F+ A P+I+FIDEID I P RD V +R++S+LL ++G+ + NV+ +AATN + +D A+ R GR ++IG+PD R +L I ADDV + A+A G GA+I
Sbjct: 213 YDDIGGCRKQMAQIREMVELPLRHPQLFKAIGIKPPRGVLMYGPPGTGKTLMARAVANETGAFFFLINGPEVMSKMAGESESNLRKAFEEAEKNAPAIIFIDEIDSIAPKRDKTN-GEVERRVVSQLLTLMDGMKARSNVVVIAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEVLRI----HTKNMKLADDV--DLEALAAETHGYVGADIA 422 HSP 4 Score: 127.872 bits (320), Expect = 2.034e-30 Identity = 70/222 (31.53%), Postives = 121/222 (54.50%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
+ DIGG + E +E+P++H G++ P+G+L +GPPG KT+ A+A E F + ++M + GESE+ R +A +++P++IFI++ID + PKR + V R++S+L+T + G+ K NV+++A TN +++D AL R GR V IG+PD R +L+I ++ + ++ +A G GA+I + E
Sbjct: 213 YDDIGGCRKQMAQIREMVELPLRHPQLFKAIGIKPPRGVLMYGPPGTGKTLMARAVANETGAFFFLINGPEVMSKMAGESESNLRKAFEEAEKNAPAIIFIDEIDSIAPKRDK-TNGEVERRVVSQLLTLMDGM--KARSNVVVIAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEVLRIHTKNMKLADD----VDLEALAAETHGYVGADIASLCSE 427
BLAST of AAA family ATPase vs. Ensembl Yeast
Match: PEX6 (AAA-peroxin; heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; mutations in human PEX6 can lead to severe peroxisomal disorders and early death [Source:SGD;Acc:S000005273]) HSP 1 Score: 133.65 bits (335), Expect = 4.392e-32 Identity = 69/179 (38.55%), Postives = 108/179 (60.34%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKS-NVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRE 3044
W DIGG + VK +++ I+MP+KH G++ GILF+GPPG KT+ A+AT L F+V +++ +GESEA R + +A E+ P +IF ++ID + PKR + S V +RI+S+L+ L G+ T + V ++ TN +DEALLRPGR ++ +G+PD ++
Sbjct: 731 WDDIGGIDFVKGEILDTIDMPLKHPELFTSGMKKRSGILFYGPPGTGKTLMAKAIATNFSLNFFSVKGPELLNMYIGESEANVRRVFQKAREAKPCVIFFDEIDSVAPKRGNQGDSGGVMDRIVSQLLAELDGMSTDAD-GVFVIGATNRPDLLDEALLRPGRFDKLLYLGIPDTDTKQ 908 HSP 2 Score: 123.25 bits (308), Expect = 6.782e-29 Identity = 67/178 (37.64%), Postives = 107/178 (60.11%), Query Frame = 3
Query: 1014 WVDIGGYESVKVMLKNEIEEPLKNGKEFVKG------VLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVS-PVSQRMMSELLMFLNGINEKPNVIFV-AATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRE 1523
W DIGG + VK + + I+ PLK+ + F G +LF+GPPG KT+ +A+AT LN S+K ++ Y+GESE +R++F +A +P ++F DEID + P R + S V R++S+LL L+G++ + +FV ATN D LD A++R GR + +G+PD +++
Sbjct: 731 WDDIGGIDFVKGEILDTIDMPLKHPELFTSGMKKRSGILFYGPPGTGKTLMAKAIATNFSLNFFSVKGPELLNMYIGESEANVRRVFQKAREAKPCVIFFDEIDSVAPKRGNQGDSGGVMDRIVSQLLAELDGMSTDADGVFVIGATNRPDLLDEALLRPGRFDKLLYLGIPDTDTKQ 908
BLAST of AAA family ATPase vs. Ensembl Yeast
Match: PEX1 (AAA-peroxin; heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis; mutations in human PEX1 can lead to severe peroxisomal disorders and early death [Source:SGD;Acc:S000001680]) HSP 1 Score: 133.265 bits (334), Expect = 5.254e-32 Identity = 101/319 (31.66%), Postives = 163/319 (51.10%), Query Frame = 3
Query: 753 DKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQ--FTGKDLTDFIDNVYRKFKEITLERLKAC----------RKDSNWIPSELLAYIADLEP-LHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILG--ITFSDQNDGF-IFADDVKENFSAIAERLSGKSGAEI 1640
DK++R +L +Y +++ M D + FSD L F+ DL F + + F ++ LER C + S + PS L E + W DIG + K +L +E P K FV G+L +G PGC KT+ A+A + GLN IS+K I+ ++G SE +R++F+RA +++P ILF DE D I P R H + V+ R++++LL ++G V +AAT+ D +D+A++R GR+ + +P + R IL + D++ G FA + + IAE+ +G SGA++
Sbjct: 604 DKHARAKLLEYFFSKNQIMKLNRDLQ---FSDLSLETEGFSPLDLEIFTEKI---FYDLQLER--DCDNVVTRELFSKSLSAFTPSALRGVKLTKETNIKWGDIGALANAKDVLLETLEWPTKYEPIFVNCPLRLRSGILLYGYPGCGKTLLASAVAQQCGLNFISVKGPEILNKFIGASEQNIRELFERAQSVKPCILFFDEFDSIAPKRGHDSTG-VTDRVVNQLLTQMDGAEGLDGVYILAATSRPDLIDSALLRPGRLDKSVICNIPTESERLDILQAIVNSKDKDTGQKKFALEKNADLKLIAEKTAGFSGADL 913 HSP 2 Score: 120.939 bits (302), Expect = 3.803e-28 Identity = 72/226 (31.86%), Postives = 118/226 (52.21%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEH-----EIISNISTIAESLTGLSGAEIVGI 3164
+ W DIG K +L+E +E P K+ L + GIL +G PGC KT+ +A+A +C L +V +I+ +G SE R + +A P ++F ++ D + PKR +S + V +R++++L+T + G E V I+A T+ +D ALLRPGRL V +P + +R IL+ + + T E +++ IAE G SGA++ G+
Sbjct: 694 IKWGDIGALANAKDVLLETLEWPTKYEPIFVNCPLRLRSGILLYGYPGCGKTLLASAVAQQCGLNFISVKGPEILNKFIGASEQNIRELFERAQSVKPCILFFDEFDSIAPKRGHDS-TGVTDRVVNQLLTQMDGAEGL--DGVYILAATSRPDLIDSALLRPGRLDKSVICNIPTESERLDILQAIVNSKDKDTGQKKFALEKNADLKLIAEKTAGFSGADLQGL 916
BLAST of AAA family ATPase vs. Ensembl Yeast
Match: RIX7 (Putative ATPase of the AAA family; required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions [Source:SGD;Acc:S000003957]) HSP 1 Score: 131.724 bits (330), Expect = 1.261e-31 Identity = 90/290 (31.03%), Postives = 151/290 (52.07%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIA--ERLSGKSGAEIVEFGRQVRLGVRRALPLDTRYTSSGILIRKCHVDSCLSGMAIEGNEISCEFQSISIG 1811
P+ A + + W ++G + V++ L I +P+K + + K GVL GPPGC KT+ +A+A E+ N ISIK ++ YVGESE +RQ+F RA A P ++F DE+D + P RD ++S S R+++ LL L+G+N++ + + ATN D +D A++R GR+ + I LP+ + E + I ++ G + DV +F I E+ + SGA++ R+ + L L ++ S + S L N++ EF+ +S+G
Sbjct: 517 PTAKREGFATVPDVTWANVGALQRVRLELNMAIVQPIKRPELYEKVGISAPGGVLLWGPPGCGKTLLAKAVANESRANFISIKGPELLNKYVGESERSIRQVFTRARASVPCVIFFDELDALVPRRDT-SLSESSSRVVNTLLTELDGLNDRRGIFVIGATNRPDMIDPAMLRPGRLDKSLFIELPN--TEEKLDIIKTLTKSHGTPLSSDV--DFEEIIRNEKCNNFSGADLAALVRESSV-----LALKRKFFQSE------EIQSVLD------NDLDKEFEDLSVG 784 HSP 2 Score: 124.79 bits (312), Expect = 1.866e-29 Identity = 84/220 (38.18%), Postives = 118/220 (53.64%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEP-------LKNGKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELL-----MFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSD--QNDGFIFADDVKENFSAIAERLSGKSGAEI 1640
+GG + V L I P L G E +GVL HGPPGC KT ALA E + ISI +++ G GESE +R +FD A +L P ++F DEID I P RD A + +R++++LL + + N KP VI + ATN D LDAA+ R+GR I + +P++VSR IL SD + DG I +F+ +A+ G GA++
Sbjct: 207 LGGMDDVVAQLMELIGLPILHPEIFLSTGVEPPRGVLLHGPPGCGKTSIANALAGELQVPFISISAPSVVSGMSGESEKKIRDLFDEARSLAPCLVFFDEIDAITPKRDGGAQREMERRIVAQLLTSMDELTMEKTNGKP-VIIIGATNRPDSLDAALRRAGRFDREICLNVPNEVSRLHILK-KMSDNLKIDGAI-------DFAKLAKLTPGFVGADL 417 HSP 3 Score: 122.479 bits (306), Expect = 8.671e-29 Identity = 72/219 (32.88%), Postives = 121/219 (55.25%), Query Frame = 3
Query: 2538 IGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKN--QNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIV 3167
+GG + V L+E I +PI H G+E P+G+L HGPPGC KT NALA E Q+ ++ ++ G+ GESE R + +A +P L+F ++ID + PKR ++ + RI+++L+T + + +K + V+I+ TN ++D AL R GR + + +P++ R ILK SD N + + I + + +A+ G GA++ +V
Sbjct: 207 LGGMDDVVAQLMELIGLPILHPEIFLSTGVEPPRGVLLHGPPGCGKTSIANALAGELQVPFISISAPSVVSGMSGESEKKIRDLFDEARSLAPCLVFFDEIDAITPKRDGGAQREMERRIVAQLLTSMDELTMEKTNGKPVIIIGATNRPDSLDAALRRAGRFDREICLNVPNEVSRLHILK-KMSD-NLKIDGAI--DFAKLAKLTPGFVGADLKALV 421 HSP 4 Score: 121.709 bits (304), Expect = 1.642e-28 Identity = 80/256 (31.25%), Postives = 134/256 (52.34%), Query Frame = 3
Query: 2481 IENTSKELDHQSQP-LHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGF--------SDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE-----MKRK 3185
I+ T+K + P + W ++G + V+ L I PIK G+ P G+L GPPGC KT+ A+A E + ++ +++ VGESE + R + ++A S P +IF +++D L+P+R + S S R+++ L+T L G+ ++ + ++ TN +D A+LRPGRL + I LP+ +++ I+K SD++F+ EII N E SGA++ +VRE +KRK
Sbjct: 515 IQPTAKREGFATVPDVTWANVGALQRVRLELNMAIVQPIKRPELYEKVGISAPGGVLLWGPPGCGKTLLAKAVANESRANFISIKGPELLNKYVGESERSIRQVFTRARASVPCVIFFDELDALVPRRDT-SLSESSSRVVNTLLTELDGLNDRR--GIFVIGATNRPDMIDPAMLRPGRLDKSLFIELPNTEEKLDIIKTLTKSHGTPLSSDVDFE---EIIRN-----EKCNNFSGADLAALVRESSVLALKRK 759
BLAST of AAA family ATPase vs. Ensembl Nematostella
Match: EDO39006 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBC8]) HSP 1 Score: 167.548 bits (423), Expect = 1.268e-43 Identity = 96/243 (39.51%), Postives = 140/243 (57.61%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQN-DGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGV 1673
PS + ++ +HW D+GG E +K LK +E PLK+ + F +G+L +GPPGCSKT+ RALATE+GLN I+IK + +VGESE +R++F +A A PSI+F DE+D I R+ S V+ R++++LL L+G+ +VIF+AATN D +D A+MR GR+ I + LP +R IL I + +G + +D + ER G SGAEI R+ L
Sbjct: 292 PSAMREVALEVPKVHWSDVGGNEMIKRKLKEAVEWPLKHPEAFQRLGIRPPRGILMYGPPGCSKTLIARALATESGLNFIAIKGPELFSKWVGESEKAVREVFLKARATAPSIVFFDELDAIAGQRNSTGGSDVNDRVLTQLLTELDGVETLKDVIFIAATNRPDMIDKALMRPGRVDRLIYVPLPCWDTRRHILEIHLARTPCEGSLDLED-------LVERTEGYSGAEIAAVCREAALAA 527 HSP 2 Score: 164.851 bits (416), Expect = 1.182e-42 Identity = 112/378 (29.63%), Postives = 191/378 (50.53%), Query Frame = 3
Query: 2253 GFVNKKVCMSLPKDTEECLKHMIYKIL-NIKSSV--EDIEELVKNLHGFTPKDIRWFLKSASVK-----------------------------YSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHNNNENVSDSLPTIKYQYFREAISA 3269
G ++++ + +P T+ + ++ +L N+ S+ EDI L ++ HG+ D+ K AS+ S DMR R + +E+ + +HW+D+GG E +K L E +E P+KH G+ P+GIL +GPPGC KT+ ALATE L + ++ VGESE A R + +A ++PS++F +++D + +R+S S+V +R+L++L+T L G+ET K +V+ +A TN +D+AL+RPGR+ ++ + LP R IL+I + +T E ++ + E G SGAEI + RE A EN+ +++ ++F +A+ A
Sbjct: 187 GRFDREIEIGIPSVTDR--RDILVTLLKNVPHSLHDEDISSLAESAHGYVGADLAAACKEASLYAFKRSLHNHDNGSSQSLEQRDARIKRELLVSSEDMRAAFRCVR--PSAMREVALEVPKVHWSDVGGNEMIKRKLKEAVEWPLKHPEAFQRLGIRPPRGILMYGPPGCSKTLIARALATESGLNFIAIKGPELFSKWVGESEKAVREVFLKARATAPSIVFFDELDAIAGQRNSTGGSDVNDRVLTQLLTELDGVETLK--DVIFIAATNRPDMIDKALMRPGRVDRLIYVPLPCWDTRRHILEIHLA----RTPCEGSLDLEDLVERTEGYSGAEIAAVCRE----AALAALQENIQAE--SVELRHFEKALMA 548 HSP 3 Score: 132.494 bits (332), Expect = 4.280e-32 Identity = 87/285 (30.53%), Postives = 143/285 (50.18%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPI-------KHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQN-VMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKR---KAQFHNNNENVSDSLPTIKYQYFREAISASCSIGTMMELLKISC-QEPSLDQLNIH 3347
+ IGG +T + E IEMP+ +G+ P+GIL +GP G KTM A+A E + F + +++ GE+EA R I ++A SPS++FI+++D L P+R ++ R+++ L+T + G+ K VM++A TN +D AL RPGR + IG+P DR IL ++ E +IS++AES G GA++ +E K HN++ S SL + RE + +S + ++ S +E +L+ +H
Sbjct: 26 FQSIGGLKTQIQAVREMIEMPLTNPELFTAYGVPPPRGILLYGPSGTGKTMIARAVANETGVHFFCINGPEVLSRYYGETEARLREIFTEAQNKSPSIVFIDELDALCPRR-DKVQNEFERRVVATLLTLMDGMHMKSTDTYVMVLAATNRPDALDPALRRPGRFDREIEIGIPSVTDRRDILVTLLKNVPHSLHDE---DISSLAESAHGYVGADLAAACKEASLYAFKRSLHNHDNGSSQSLEQRDARIKRELLVSSEDMRAAFRCVRPSAMREVALEVPKVH 306 HSP 4 Score: 124.405 bits (311), Expect = 1.728e-29 Identity = 88/284 (30.99%), Postives = 142/284 (50.00%), Query Frame = 3
Query: 1023 IGGYESVKVMLKNEIEEPLKNGKEFV-------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPN---VIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRALPLDTRYTSSGILIRKCHVDSCLSGMAIEGNEISCEFQSISIGSINDNVLKVP 1841
IGG ++ ++ IE PL N + F +G+L +GP G KTM RA+A ETG++ I ++ Y GE+E LR+IF A PSI+FIDE+D + P RD + + +R+++ LL ++G++ K V+ +AATN D LD A+ R GR IEIG+P R IL +N D E+ S++AE G GA++ ++ L +R+L +S + R + L + ++ F+ + ++ + L+VP
Sbjct: 29 IGGLKTQIQAVREMIEMPLTNPELFTAYGVPPPRGILLYGPSGTGKTMIARAVANETGVHFFCINGPEVLSRYYGETEARLREIFTEAQNKSPSIVFIDELDALCPRRDKVQ-NEFERRVVATLLTLMDGMHMKSTDTYVMVLAATNRPDALDPALRRPGRFDREIEIGIPSVTDRRDILVTLL--KNVPHSLHD---EDISSLAESAHGYVGADLAAACKEASLYAFKRSLHNHDNGSSQSLEQRDARIKREL---LVSSEDMRAAFRCVRPSAMREVALEVP 303
BLAST of AAA family ATPase vs. Ensembl Nematostella
Match: EDO36218 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ61]) HSP 1 Score: 157.532 bits (397), Expect = 2.069e-39 Identity = 134/480 (27.92%), Postives = 220/480 (45.83%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-------------SNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLT---------------DFIDNVYRKFKEITLERLKACRKDSNWI-----PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR--DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEF-GRQVRLGVRRALPLD 1694
+++ GP G GK + + +A+E FF IN L +E + K + A K+ ++ I++ + + ++D L + S + + PNS V RR +EV + I D R E+ + + M DD + ++ + + + + G D+ D ID L+ L D + PS L + ++ + W DIGG E VK L+ ++ P+++ +F+K GVLF+GPPGC KT+ +A+A E N ISIK ++ + GESE +R +FD+A + P +LF DE+D I SR + + R+++++L ++G+N K NV + ATN D +D A++R GR+ I I LPD SR SIL A DV ++ +A+ G SGA++ E R +L +R A+ D
Sbjct: 236 ILLFGPPGTGKTLMARAVANET-GAFFFLINGPEIMSKLAGESESNLRKAFEE---AEKNSPAIIFIDEIDAIAPKRDKTHGEVERRIVSQLLTLMDGLKQRSHVIVMAATNRPNSVDVALRRFGRFDREVDIGIPDATGRLEILRI---HTKNMKLGDDVDLEQIA-AETHGYVGSDVASLCSEAALQQIREKMDLIDLEDETIDAEVLDSLAVSMDDFRYAMGVSNPSALRETVVEVPNVSWDDIGGLEGVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDVFDKARSAAPCVLFFDELDSIAKSRGGNVGDGGGAADRVINQVLTEMDGMNVKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDDGSRSSILKANLRKSP----IAKDVDLDY--VAKVTHGFSGADLTEICQRACKLAIREAIETD 701 HSP 2 Score: 140.584 bits (353), Expect = 5.276e-34 Identity = 122/479 (25.47%), Postives = 214/479 (44.68%), Query Frame = 3
Query: 1992 EHLESTMQQTIFIRLSDKLATKEISEVIEVINHGNSKRK---DMVVVVDGMRKMSDDFCEAVQLTLINEAPNVKVMMIFEDLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVE-DIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSRE----------------------IENTS--KELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGI--------VRE-----MKRKAQFHNNNE-----NVSDSLPTIKYQYFREAI 3263
E E IFI D +A K HG +R+ ++ ++DG+++ S + + N +V V + + G +++V + +P D L+ + N+K + D+E++ HG+ D+ A+++ R M LI+ E + N S +E + + W DIGG E VK L E ++ P++H G+ KG+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R + +A ++P ++F +++D + R + +R++++++T + G+ KK NV I+ TN +D A+LRPGRL ++ I LPD R SILK + ++ +A+ G SGA++ I +RE + R+ Q +N + D +P I+ +F EA+
Sbjct: 286 EEAEKNSPAIIFIDEIDAIAPKRDK------THGEVERRIVSQLLTLMDGLKQRS----HVIVMAATNRPNSVDVAL----------RRFGRFDREVDIGIP-DATGRLEILRIHTKNMKLGDDVDLEQIAAETHGYVGSDVASLCSEAALQQIREKM-DLIDLEDETIDAEVLDSLAVSMDDFRYAMGVSNPSALRETVVEVPNVSWDDIGGLEGVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDVFDKARSAAPCVLFFDELDSIAKSRGGNVGDGGGAADRVINQVLTEMDGMNVKK--NVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDDGSRSSILKANLRKSPIAKD----VDLDYVAKVTHGFSGADLTEICQRACKLAIREAIETDINREKQRVDNPDLDMEVEDEDPVPEIRKDHFEEAM 736 HSP 3 Score: 128.257 bits (321), Expect = 3.846e-30 Identity = 81/230 (35.22%), Postives = 119/230 (51.74%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
L + + DIGG +K +E PL++ + F +G+L GPPG KT+ RA+A ETG I IM GESE LR+ F+ A P+I+FIDEID I P RD V +R++S+LL ++G+ ++ +VI +AATN + +D A+ R GR ++IG+PD R IL I + G DDV + IA G G+++ + L
Sbjct: 193 LNEVGYDDIGGCRKQLAQIKEMVELPLRHPQLFKAIGVKPPRGILLFGPPGTGKTLMARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNSPAIIFIDEIDAIAPKRDKTH-GEVERRIVSQLLTLMDGLKQRSHVIVMAATNRPNSVDVALRRFGRFDREVDIGIPDATGRLEILRIHTKNMKLG----DDV--DLEQIAAETHGYVGSDVASLCSEAAL 415 HSP 4 Score: 127.102 bits (318), Expect = 9.719e-30 Identity = 74/222 (33.33%), Postives = 121/222 (54.50%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
+ DIGG + E +E+P++H G++ P+GIL GPPG KT+ A+A E F + +IM + GESE+ R +A ++SP++IFI++ID + PKR + V RI+S+L+T + G+ K+ +V+++A TN ++VD AL R GR V IG+PD R IL+I ++ + ++ IA G G+++ + E
Sbjct: 198 YDDIGGCRKQLAQIKEMVELPLRHPQLFKAIGVKPPRGILLFGPPGTGKTLMARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNSPAIIFIDEIDAIAPKR-DKTHGEVERRIVSQLLTLMDGL--KQRSHVIVMAATNRPNSVDVALRRFGRFDREVDIGIPDATGRLEILRIHTKNMKLGDD----VDLEQIAAETHGYVGSDVASLCSE 412
BLAST of AAA family ATPase vs. Ensembl Nematostella
Match: EDO35705 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKV3]) HSP 1 Score: 135.191 bits (339), Expect = 8.803e-33 Identity = 81/244 (33.20%), Postives = 129/244 (52.87%), Query Frame = 3
Query: 969 PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRR 1679
PS A + + W ++G + V+ L I P+++ +EF G+L GPPGC KT+ +A+A E+G+N IS+K ++ YVGESE +RQ+F RA P ++F DE+D + P R + S S R++++LL ++G+ + +V + ATN D +D AVMR GR+ + +GLP R+ IL + F+ DV A R +G SGA++ R+ + R
Sbjct: 274 PSAKREGFATVPDVTWANVGALKDVREELTMAILAPVQHPEEFSALGLTHPPGILLAGPPGCGKTLLAKAIANESGINFISVKGPELLNMYVGESERAVRQVFQRARNSAPCVIFFDELDSLCPRRSGSSESGSSVRVVNQLLTEMDGLQARKHVFIMGATNRPDIIDPAVMRPGRLDKVLYVGLPSAHDRQDILRTLTKEGRKPFLLP-DVHLGLLAEDTRCNGFSGADLAALVREASMAALR 516 HSP 2 Score: 121.709 bits (304), Expect = 2.063e-28 Identity = 67/180 (37.22%), Postives = 101/180 (56.11%), Query Frame = 3
Query: 1086 GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPN--VIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGI--------------TFSDQNDGFIFAD 1577
G G L HGPPGC KT+ A+A E + + + + I+ G GESE +R++F AVA P ILF+DEID I P R++ A + +R++S+LL ++ +N P+ V+ + ATN D LD A+ R+GR I +G+PD+ +R SIL + + S Q G++ AD
Sbjct: 11 GVTPTTGFLLHGPPGCGKTLLAHAIAGELEMPFLKLAATEIVSGVSGESEEKVRELFTSAVAQAPCILFLDEIDAITPKREN-ASKDMERRIVSQLLSCMDDLNNNPDAHVLVIGATNRPDSLDPALRRAGRFDREISMGIPDESARASILRVMCKQLKLSADFSFLSLSRQTPGYVGAD 189 HSP 3 Score: 120.939 bits (302), Expect = 3.977e-28 Identity = 78/233 (33.48%), Postives = 125/233 (53.65%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKI----GFSDLNFQTEHEIISNISTIAESL--TGLSGAEIVGIVREMKRKA 3188
W ++G + V+ L I P++H GL P GIL GPPGC KT+ A+A E + +V +++ VGESE A R + +A S+P +IF +++D L P+RS +S+S R++++L+T + G++ +K +V I+ TN +D A++RPGRL ++ +GLP DR+ IL+ G H + +AE G SGA++ +VRE A
Sbjct: 289 WANVGALKDVREELTMAILAPVQHPEEFSALGLTHPPGILLAGPPGCGKTLLAKAIANESGINFISVKGPELLNMYVGESERAVRQVFQRARNSAPCVIFFDELDSLCPRRSGSSESGSSVRVVNQLLTEMDGLQARK--HVFIMGATNRPDIIDPAVMRPGRLDKVLYVGLPSAHDRQDILRTLTKEGRKPFLLPDVH-----LGLLAEDTRCNGFSGADLAALVREASMAA 514 HSP 4 Score: 120.553 bits (301), Expect = 5.007e-28 Identity = 58/191 (30.37%), Postives = 109/191 (57.07%), Query Frame = 3
Query: 2601 GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
G+ G L HGPPGC KT+ +A+A E ++ + ++I+ G+ GESE R + + A+ +P ++F+++ID + PKR + SK ++ RI+S+L++ + + + +V+++ TN ++D AL R GR + +G+PD+ R SIL++ L + +S ++ G GA+++ + RE
Sbjct: 11 GVTPTTGFLLHGPPGCGKTLLAHAIAGELEMPFLKLAATEIVSGVSGESEEKVRELFTSAVAQAPCILFLDEIDAITPKRENASK-DMERRIVSQLLSCMDDLNNNPDAHVLVIGATNRPDSLDPALRRAGRFDREISMGIPDESARASILRVMCKQLKLSADFSFLS----LSRQTPGYVGADLMALTRE 196
BLAST of AAA family ATPase vs. Ensembl Nematostella
Match: EDO42053 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2J4]) HSP 1 Score: 129.028 bits (323), Expect = 1.227e-32 Identity = 92/295 (31.19%), Postives = 145/295 (49.15%), Query Frame = 3
Query: 2379 LHGFTPKDIRWFLKSAS-VKYSR---------------TDMRQLIEFSREIENTSKELDHQSQPLHWTDIGG-YETVKTMLIENIEMPIKHG--------LEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
L FT ++W +++ + SR D QL E+ E+ + +D S P+ W+ IGG +ET++ + E I +PI+ L PKG+L HGPPGC KTM A A E + N+ S + GES+ A+ S AL+ P +IFI++ID + R S + ++ M+ G+ T+ N V+++ TN +VD+A+LR R+ +GLPD++ RE IL+I N + E+ N+ +A G SG+++ REM R A
Sbjct: 18 LGAFTYYGVKWLMEAMDPTRKSRMAAQKQAEQLLKLIGADGVQLSEY--ELAIAADLVDPLSLPIQWSHIGGLHETIQD-VKETIILPIQKSHIFSKSKLLSPPKGVLLHGPPGCGKTMIAKATAKEAGCRFLNLQVSSLTDKWYGESQKLAAAVFSLALKLQPCIIFIDEIDSFLRAR-DKSDHEATAMMKAQFMSLWDGLVTEPNCQVIVMGATNRPQDVDKAILR--RMPAAFHVGLPDERQREEILRIILQSEN--VDGEVFLNLDELAAITCGFSGSDL----REMCRTA 300 HSP 2 Score: 128.642 bits (322), Expect = 1.647e-32 Identity = 88/252 (34.92%), Postives = 124/252 (49.21%), Query Frame = 3
Query: 981 LAYIADLE-----PLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK--------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR---DHLAVSPVSQRMMSELLMFLNGINEKPN--VIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQN-DGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRR 1679
LA ADL P+ W IGG +K I P++ F K GVL HGPPGC KTM +A A E G ++++ S++ + GES+ L +F A+ LQP I+FIDEID +R DH A + + + MS +G+ +PN VI + ATN +D A++R RM +GLPD+ RE IL I +N DG +F N +A G SG+++ E R + R
Sbjct: 66 LAIAADLVDPLSLPIQWSHIGGLHETIQDVKETIILPIQKSHIFSKSKLLSPPKGVLLHGPPGCGKTMIAKATAKEAGCRFLNLQVSSLTDKWYGESQKLAAAVFSLALKLQPCIIFIDEIDSFLRARDKSDHEATAMMKAQFMS----LWDGLVTEPNCQVIVMGATNRPQDVDKAILR--RMPAAFHVGLPDERQREEILRIILQSENVDGEVFL-----NLDELAAITCGFSGSDLREMCRTAAMNCVR 306
BLAST of AAA family ATPase vs. Ensembl Nematostella
Match: EDO48602 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ14]) HSP 1 Score: 124.79 bits (312), Expect = 2.925e-29 Identity = 76/217 (35.02%), Postives = 124/217 (57.14%), Query Frame = 3
Query: 903 YRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFV-----KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVS-PVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLP-DQVSRESIL 1532
++ F+E L+ L+A D+ P I D+ W D+GG +SVK + + I+ PL + + F GVL +GPPG KT+ +A+ATE LN +S+K ++ YVG+SE +R++F RA A P ++F DE+D + P+R S V R++++LL L+G++ +V + ATN D LD A++R GR + +G+ D ++ S+L
Sbjct: 372 FKDFQE-ALDALQASHADAIGAPK-----IPDIS---WKDVGGLDSVKEEILDTIQLPLLHPELFAAGLRRSGVLLYGPPGTGKTLMAKAVATECSLNFLSVKGPELINMYVGQSEQNVREVFSRAQAASPCVIFFDELDSLAPNRGRSGDSGGVMDRVVAQLLAELDGLHSTCDVFVIGATNRPDLLDPALLRPGRFDKLLYLGVSKDHHAQLSVL 579 HSP 2 Score: 119.013 bits (297), Expect = 1.716e-27 Identity = 72/243 (29.63%), Postives = 128/243 (52.67%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIKH------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRS-SNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLP-DQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTG--LSGAEIVGIVREMKRKAQFHNNNENVSD 3221
+ W D+GG ++VK +++ I++P+ H GL G+L +GPPG KT+ A+ATEC L +V +++ VG+SE R + S+A +SP +IF +++D L P R S V +R++++L+ L G+ + +V ++ TN +D ALLRPGR ++ +G+ D + S+LK F + + + + +LTG L +++ M+R Q N + ++
Sbjct: 398 ISWKDVGGLDSVKEEILDTIQLPLLHPELFAAGLR-RSGVLLYGPPGTGKTLMAKAVATECSLNFLSVKGPELINMYVGQSEQNVREVFSRAQAASPCVIFFDELDSLAPNRGRSGDSGGVMDRVVAQLLAELDGLHS--TCDVFVIGATNRPDLLDPALLRPGRFDKLLYLGVSKDHHAQLSVLKALTRKFTFSADFRLEEFANKLPLNLTGADLYAMASDALLQAMRRIIQETGGNADAAE 637
BLAST of AAA family ATPase vs. Ensembl Medaka
Match: vcp (valosin containing protein [Source:NCBI gene;Acc:101174924]) HSP 1 Score: 154.451 bits (389), Expect = 6.794e-38 Identity = 131/463 (28.29%), Postives = 218/463 (47.08%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-SNLNEEFH------------LIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDF-----IDNVYRKFKEITLE----------RLKACRKDSNWI-----PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR--DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVE 1646
++++GP G GK + + +A+E FF IN L +E + K + A K+ ++ +++ + E+ H ++D L + + + + PNS RR +EV + I D R E+ + + M DD + ++ + N+ + G DL + + +K I LE L D W PS L I ++ + W DIGG E VK L+ ++ P+++ +F+K GVLF+GPPGC KT+ +A+A E N ISIK ++ + GESE +R+IFD+A P +LF DE+D I +R + + R+++++L ++G++ K NV + ATN D +D A++R GR+ I I LPD+ SR SIL + DV +F +A+ +G SGA++ E
Sbjct: 249 ILLYGPPGTGKTLIARAVANET-GAFFFLINGPEIMSKLAGESESNLRKAFEE---AEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQI---HTKNMKLADDVDLEQVA-NETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFKWALSQSNPSALRETIVEVPNITWDDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNVGDGGGAADRVINQILTEMDGMSSKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRISILKANLRKSP----ISKDVDLDF--LAKMTNGFSGADLTE 697 HSP 2 Score: 140.198 bits (352), Expect = 2.051e-33 Identity = 97/325 (29.85%), Postives = 161/325 (49.54%), Query Frame = 3
Query: 2223 DLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVR 3170
DL+ V ++ G V + +E L+ + K+ I E I+ V N T D +W L S+++ L E E+ N + W DIGG E VK L E ++ P++H G+ KG+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R I +A +++P ++F +++D + R + +R++++++T + G+ +KK NV I+ TN +D A+LRPGRL ++ I LPD+K R SILK + ++ +A+ G SGA++ I +
Sbjct: 403 DLEQVANETHGHVGADLAALC---SEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFKWAL-------SQSNPSALRETIVEVPNIT-----------WDDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNVGDGGGAADRVINQILTEMDGMSSKK--NVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRISILKANLRKSPISKD----VDLDFLAKMTNGFSGADLTEICQ 700 HSP 3 Score: 129.798 bits (325), Expect = 3.938e-30 Identity = 81/239 (33.89%), Postives = 125/239 (52.30%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKN-------GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL-GVRRALPL 1691
L + + DIGG +K +E PL++ G + +G+L +GPPG KT+ RA+A ETG I IM GESE LR+ F+ A P+I+FIDE+D I P R+ V +R++S+LL ++G+ ++ +VI +AATN + +D A+ R GR ++IG+PD R IL I + ADDV + +A G GA++ + L +R+ + L
Sbjct: 206 LNEVGYDDIGGVRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTH-GEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMK----LADDV--DLEQVANETHGHVGADLAALCSEAALQAIRKKMDL 437 HSP 4 Score: 126.716 bits (317), Expect = 3.680e-29 Identity = 72/227 (31.72%), Postives = 124/227 (54.63%), Query Frame = 3
Query: 2529 WTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
+ DIGG + E +E+P++H G++ P+GIL +GPPG KT+ A+A E F + +IM + GESE+ R +A +++P++IFI+++D + PKR + V RI+S+L+T + G+ K+ +V+++A TN +++D AL R GR V IG+PD R IL+I ++ + ++ +A G GA++ + E +A
Sbjct: 211 YDDIGGVRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKR-EKTHGEVERRIVSQLLTLMDGL--KQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADD----VDLEQVANETHGHVGADLAALCSEAALQA 430
BLAST of AAA family ATPase vs. Ensembl Medaka
Match: spata5l1 (spermatogenesis associated 5 like 1 [Source:NCBI gene;Acc:101172062]) HSP 1 Score: 132.88 bits (333), Expect = 3.190e-31 Identity = 97/321 (30.22%), Postives = 152/321 (47.35%), Query Frame = 3
Query: 2253 GFVNKKVCMSLPKDTEE-CLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIE---FSREIENTSK---------ELDHQSQP-LHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCE-RILSELMTFLSGIETKK------------------------------NQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKI 3059
G +K+V + +P T+ + ++++ + + +V ++ EL + G+ D+ + A + R + + E R E K L P + W IGG + VK L ++IE P+KH GL P+G+L GPPGC KT V A AT +V +D+ VG+SEAA + QA +PS++F+++ID LI RS+N N + R+LS L+ + GI K N++VM+VA TN +D ALLRPGR+ I+ + PDQ+ R SIL++
Sbjct: 354 GRFDKEVVIGVPTLTQRLAILKVLFEKIPVCPTV-NLAELAQRTTGYVGADLNALCREAIMHAVRENAKGSAEQKVVMRHFEEALKVVSPSCLRSSLGRTDLPRVLWEHIGGLDEVKLKLKQSIEWPMKHPEAFVRLGLSRPRGVLLFGPPGCAKTTLVKAAATSSSCAFLSVSSADLYSSYVGDSEAALAQLFRQARACAPSILFLDEIDSLIGSRSTNETQNSEQTRLLSVLLNEMDGIGVKTTERRGAEKTLQAEGMTKSGAQEELEWEEVCNKDVMVVAATNRPDCLDSALLRPGRMDHIIYVPPPDQQARLSILRV 673 HSP 2 Score: 124.79 bits (312), Expect = 1.017e-28 Identity = 82/221 (37.10%), Postives = 113/221 (51.13%), Query Frame = 3
Query: 996 DLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQ-RMMSELLMFLNGINEKP--------------------------------NVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGI 1538
DL + W IGG + VK+ LK IE P+K+ + FV+ GVL GPPGC+KT V+A AT + +S+ + + YVG+SE L Q+F +A A PSILF+DEID + SR Q R++S LL ++GI K +V+ VAATN D LD+A++R GRM I + PDQ +R SIL +
Sbjct: 453 DLPRVLWEHIGGLDEVKLKLKQSIEWPMKHPEAFVRLGLSRPRGVLLFGPPGCAKTTLVKAAATSSSCAFLSVSSADLYSSYVGDSEAALAQLFRQARACAPSILFLDEIDSLIGSRSTNETQNSEQTRLLSVLLNEMDGIGVKTTERRGAEKTLQAEGMTKSGAQEELEWEEVCNKDVMVVAATNRPDCLDSALLRPGRMDHIIYVPPPDQQARLSILRV 673 HSP 3 Score: 122.094 bits (305), Expect = 7.846e-28 Identity = 88/284 (30.99%), Postives = 143/284 (50.35%), Query Frame = 3
Query: 2472 SREIEN--TSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQ---ALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHNNNENVSDSL-PTIKYQYFREA---ISASC 3275
S EI N T + Q Q +GG E V L E +++P+ + G+ P+G+L GPPG KT V +A E + V +++ GESEAA RA+ + A E P ++F++++D L P+R+++S R++++L+T + GI+ ++ ++V TN ++D AL RPGR V IG+P R +ILK+ F + N++ +A+ TG GA++ + RE A H EN S + ++F EA +S SC
Sbjct: 173 SVEISNIQTVRHFRSQLQDQQTIPLGGLEEVTASLKEMLQLPLLYPRILSSVGVTCPRGMLLVGPPGVGKTQLVQKVAGEVGASLVPVRGPELVGSRPGESEAALRAVFERARSAAEDGPCVLFLDELDSLFPRRTASSAPE--NRLVAQLLTLMDGID--QSDRFLVVGATNRPDSLDPALRRPGRFDKEVVIGVPTLTQRLAILKVLFEKIPVCP----TVNLAELAQRTTGYVGADLNALCRE----AIMHAVRENAKGSAEQKVVMRHFEEALKVVSPSC 444 HSP 4 Score: 118.627 bits (296), Expect = 8.686e-27 Identity = 74/235 (31.49%), Postives = 120/235 (51.06%), Query Frame = 3
Query: 987 YIADLEPLHWVDIGGYESVKVMLKNEIEEPL-------KNGKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQ---PSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQV 1661
+ + L+ + +GG E V LK ++ PL G +G+L GPPG KT V+ +A E G +L+ ++ ++ GESE LR +F+RA + P +LF+DE+D +FP R A S R++++LL ++GI++ + V ATN D LD A+ R GR + IG+P R +IL + F + V N + +A+R +G GA++ R+
Sbjct: 185 FRSQLQDQQTIPLGGLEEVTASLKEMLQLPLLYPRILSSVGVTCPRGMLLVGPPGVGKTQLVQKVAGEVGASLVPVRGPELVGSRPGESEAALRAVFERARSAAEDGPCVLFLDELDSLFPRRT--ASSAPENRLVAQLLTLMDGIDQSDRFLVVGATNRPDSLDPALRRPGRFDKEVVIGVPTLTQRLAILKVLF----EKIPVCPTV--NLAELAQRTTGYVGADLNALCREA 411
BLAST of AAA family ATPase vs. Ensembl Medaka
Match: nvl (nuclear VCP like [Source:NCBI gene;Acc:101154831]) HSP 1 Score: 131.339 bits (329), Expect = 1.163e-30 Identity = 82/242 (33.88%), Postives = 124/242 (51.24%), Query Frame = 3
Query: 957 SNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQV 1661
S+ PS A + + W +G + V+ L I P+++ + F GVL GPPGC KT+ +A+A E+GLN IS+K ++ YVGESE +RQ+F R P ++F DEID + P R S S R++++LL ++G+ + V +AATN D +D AV+R GR+ + +GLP R +IL + + DV A ER S SGA++ R+
Sbjct: 515 SSVQPSAKREGFATVPDVSWEHVGALQDVREELTMAILAPVRSPELFRALGLGAPSGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRGRNSAPCVIFFDEIDALCPRRSGHE-SGASIRVVNQLLTEMDGLETRRQVFIMAATNRPDIIDPAVLRPGRLDKTLYVGLPGPADRCAIL-LAITKGGTRPQLGPDVHLEEIASDERCSSFSGADLTALVREA 754 HSP 2 Score: 130.183 bits (326), Expect = 2.916e-30 Identity = 84/262 (32.06%), Postives = 136/262 (51.91%), Query Frame = 3
Query: 2523 LHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIA--ESLTGLSGAEIVGIVREMKRKAQFHNNNENVSDSLPTIKYQYFREAISASCSI 3281
+ W +G + V+ L I P++ GL P G+L GPPGC KT+ A+A E L +V +++ VGESE A R + + S+P +IF ++ID L P+RS + +S R++++L+T + G+ET++ V I+A TN +D A+LRPGRL + +GLP DR +IL + + + + ++ IA E + SGA++ +VRE A + D L K Q EA+ + C++
Sbjct: 532 VSWEHVGALQDVREELTMAILAPVRSPELFRALGLGAPSGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRGRNSAPCVIFFDEIDALCPRRSGH-ESGASIRVVNQLLTEMDGLETRR--QVFIMAATNRPDIIDPAVLRPGRLDKTLYVGLPGPADRCAIL-LAITKGGTRPQLGPDVHLEEIASDERCSSFSGADLTALVREASVNA--------LKDYL---KSQQGGEALPSGCTV 778 HSP 3 Score: 125.946 bits (315), Expect = 5.352e-29 Identity = 75/201 (37.31%), Postives = 111/201 (55.22%), Query Frame = 3
Query: 957 SNWIPSELLAYIADLEPLHWVDIGGYESV-----KVMLKNEIEEPLKN-GKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKP---NVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESIL 1532
+ W P EL E D+GG E K+++ E + G +G L HGPPGC KT+ +A+A E L L+ + ++ G GESE LR++FD+AV+ P ILFIDEID I P R+ +A + +RM+++LL ++ +N P V+ + ATN D LD A+ R+GR I +G+PD+ +R IL
Sbjct: 212 AKWKPLELQYPCVTFE-----DVGGNEEALTEVCKLLIYMHHPEVFQQLGVVPPRGFLLHGPPGCGKTLLAQAVAGELQLPLLKVSAPELVSGVSGESEQKLRELFDQAVSSSPCILFIDEIDAITPKRE-VASKDMERRMVAQLLTCMDDLNSLPVTVQVMIIGATNRPDSLDPALRRAGRFDREISLGIPDEAARLRIL 406 HSP 4 Score: 123.635 bits (309), Expect = 2.865e-28 Identity = 75/238 (31.51%), Postives = 126/238 (52.94%), Query Frame = 3
Query: 2496 KELDHQSQPLHWTDIGGYETVKT------MLIENIEMPIKHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIET-KKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA 3188
K L+ Q + + D+GG E T + + + E+ + G+ P+G L HGPPGC KT+ A+A E QL + V +++ G+ GESE R + QA+ SSP ++FI++ID + PKR SK ++ R++++L+T + + + VMI+ TN ++D AL R GR + +G+PD+ R IL+ L + + + +A G GA+++ + RE A
Sbjct: 215 KPLELQYPCVTFEDVGGNEEALTEVCKLLIYMHHPEVFQQLGVVPPRGFLLHGPPGCGKTLLAQAVAGELQLPLLKVSAPELVSGVSGESEQKLRELFDQAVSSSPCILFIDEIDAITPKREVASK-DMERRMVAQLLTCMDDLNSLPVTVQVMIIGATNRPDSLDPALRRAGRFDREISLGIPDEAARLRILETLCRKLTLPADFDFLK----LARLTPGYVGADLMALCREAAMNA 447
BLAST of AAA family ATPase vs. Ensembl Medaka
Match: ENSORLT00000040582.1 (ATPase family AAA domain containing 1 [Source:NCBI gene;Acc:101174089]) HSP 1 Score: 122.094 bits (305), Expect = 1.898e-29 Identity = 81/231 (35.06%), Postives = 114/231 (49.35%), Query Frame = 3
Query: 1014 WVDIGGYESVKVMLKNEIEEPLKNGKEFV--------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR---DHLAVSPVSQRMMSELLMFLNGINEKP--NVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
W DI G + V LK + P++ F KGVL +GPPGC KT+ +A A E G I+++PST+ + GES+ L +F AV LQPSI+FIDEID SR DH A + + + MS +G++ VI + ATN LD+A++R RM I P RE IL + D+ D + N +A+ G SG+++ E R L
Sbjct: 94 WRDIAGLDEVITELKETVILPVQKRHLFQGSRLLQPPKGVLLYGPPGCGKTLIAKATAKEAGFRFINLQPSTLTDKWYGESQKLAAAVFSLAVKLQPSIIFIDEIDSFLRSRSSSDHEATAMMKAQFMS----LWDGLDTDHYCQVIIMGATNRPQDLDSAILR--RMPTRFHINQPSAKQREKILELILQDE------GVDSQVNLLDVAKETDGFSGSDLREMCRDAAL 312 HSP 2 Score: 120.168 bits (300), Expect = 8.764e-29 Identity = 83/269 (30.86%), Postives = 133/269 (49.44%), Query Frame = 3
Query: 2502 LDHQSQPLHWTDIGGYETVKTMLIENIEMPIKHG--------LEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQF-------HNNNENVSDSLPTIKYQYFREAI 3263
+D S + W DI G + V T L E + +P++ L+ PKG+L +GPPGC KT+ A A E + N+ PS + GES+ A+ S A++ PS+IFI++ID + RSS S + ++ M+ G++T V+I+ TN ++D A+LR R+ I P K RE IL++ D ++ N+ +A+ G SG+++ REM R A HN +E+ D + I+ ++AI
Sbjct: 85 VDPLSMQITWRDIAGLDEVITELKETVILPVQKRHLFQGSRLLQPPKGVLLYGPPGCGKTLIAKATAKEAGFRFINLQPSTLTDKWYGESQKLAAAVFSLAVKLQPSIIFIDEIDSFLRSRSS-SDHEATAMMKAQFMSLWDGLDTDHYCQVIIMGATNRPQDLDSAILR--RMPTRFHINQPSAKQREKILELILQDEGVDSQ----VNLLDVAKETDGFSGSDL----REMCRDAALLCVRDFVHNQSES-EDYIRPIQQSDLQKAI 341
BLAST of AAA family ATPase vs. Ensembl Medaka
Match: ENSORLT00000043214.1 (ATPase family AAA domain containing 1 [Source:NCBI gene;Acc:101174089]) HSP 1 Score: 122.094 bits (305), Expect = 1.898e-29 Identity = 81/231 (35.06%), Postives = 114/231 (49.35%), Query Frame = 3
Query: 1014 WVDIGGYESVKVMLKNEIEEPLKNGKEFV--------KGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR---DHLAVSPVSQRMMSELLMFLNGINEKP--NVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRL 1667
W DI G + V LK + P++ F KGVL +GPPGC KT+ +A A E G I+++PST+ + GES+ L +F AV LQPSI+FIDEID SR DH A + + + MS +G++ VI + ATN LD+A++R RM I P RE IL + D+ D + N +A+ G SG+++ E R L
Sbjct: 94 WRDIAGLDEVITELKETVILPVQKRHLFQGSRLLQPPKGVLLYGPPGCGKTLIAKATAKEAGFRFINLQPSTLTDKWYGESQKLAAAVFSLAVKLQPSIIFIDEIDSFLRSRSSSDHEATAMMKAQFMS----LWDGLDTDHYCQVIIMGATNRPQDLDSAILR--RMPTRFHINQPSAKQREKILELILQDE------GVDSQVNLLDVAKETDGFSGSDLREMCRDAAL 312 HSP 2 Score: 120.168 bits (300), Expect = 8.764e-29 Identity = 83/269 (30.86%), Postives = 133/269 (49.44%), Query Frame = 3
Query: 2502 LDHQSQPLHWTDIGGYETVKTMLIENIEMPIKHG--------LEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQF-------HNNNENVSDSLPTIKYQYFREAI 3263
+D S + W DI G + V T L E + +P++ L+ PKG+L +GPPGC KT+ A A E + N+ PS + GES+ A+ S A++ PS+IFI++ID + RSS S + ++ M+ G++T V+I+ TN ++D A+LR R+ I P K RE IL++ D ++ N+ +A+ G SG+++ REM R A HN +E+ D + I+ ++AI
Sbjct: 85 VDPLSMQITWRDIAGLDEVITELKETVILPVQKRHLFQGSRLLQPPKGVLLYGPPGCGKTLIAKATAKEAGFRFINLQPSTLTDKWYGESQKLAAAVFSLAVKLQPSIIFIDEIDSFLRSRSS-SDHEATAMMKAQFMSLWDGLDTDHYCQVIIMGATNRPQDLDSAILR--RMPTRFHINQPSAKQREKILELILQDEGVDSQ----VNLLDVAKETDGFSGSDL----REMCRDAALLCVRDFVHNQSES-EDYIRPIQQSDLQKAI 341
BLAST of AAA family ATPase vs. Planmine SMEST
Match: SMESG000027037.1 (SMESG000027037.1) HSP 1 Score: 2267.66 bits (5875), Expect = 0.000e+0 Identity = 1108/1108 (100.00%), Postives = 1108/1108 (100.00%), Query Frame = 3
Query: 27 MNISVVDYIGLELNHLKVNLFEKSAITQFLQNPFKLGQIYLPFSVNTFSVIKAKDEFYIYEFMKKSKSIKITEIASNIISYSIDFPVFQTAKDCEIEFPIFKNLKYPLFGLEEVENELKIMLNGVGNSVVVMHGPRGCGKKSLMKRLADEMPNKSFFWINCLTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINSSNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRRRKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDFIDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRRALPLDTRYTSSGILIRKCHVDSCLSGMAIEGNEISCEFQSISIGSINDNVLKVPIETGNEFNLNRDELFDCEDLVEDISSFLLDDVGKCLIVCDISDCGKMSLVEHLESTMQQTIFIRLSDKLATKEISEVIEVINHGNSKRKDMVVVVDGMRKMSDDFCEAVQLTLINEAPNVKVMMIFEDLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHNNNENVSDSLPTIKYQYFREAISASCSIGTMMELLKISCQEPSLDQLNIHK 3350
MNISVVDYIGLELNHLKVNLFEKSAITQFLQNPFKLGQIYLPFSVNTFSVIKAKDEFYIYEFMKKSKSIKITEIASNIISYSIDFPVFQTAKDCEIEFPIFKNLKYPLFGLEEVENELKIMLNGVGNSVVVMHGPRGCGKKSLMKRLADEMPNKSFFWINCLTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINSSNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRRRKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDFIDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRRALPLDTRYTSSGILIRKCHVDSCLSGMAIEGNEISCEFQSISIGSINDNVLKVPIETGNEFNLNRDELFDCEDLVEDISSFLLDDVGKCLIVCDISDCGKMSLVEHLESTMQQTIFIRLSDKLATKEISEVIEVINHGNSKRKDMVVVVDGMRKMSDDFCEAVQLTLINEAPNVKVMMIFEDLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHNNNENVSDSLPTIKYQYFREAISASCSIGTMMELLKISCQEPSLDQLNIHK
Sbjct: 1 MNISVVDYIGLELNHLKVNLFEKSAITQFLQNPFKLGQIYLPFSVNTFSVIKAKDEFYIYEFMKKSKSIKITEIASNIISYSIDFPVFQTAKDCEIEFPIFKNLKYPLFGLEEVENELKIMLNGVGNSVVVMHGPRGCGKKSLMKRLADEMPNKSFFWINCLTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINSSNLNEEFHLIIDNLVENSQIDIKILLEIPNSDSVDFRRRKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDFIDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRRALPLDTRYTSSGILIRKCHVDSCLSGMAIEGNEISCEFQSISIGSINDNVLKVPIETGNEFNLNRDELFDCEDLVEDISSFLLDDVGKCLIVCDISDCGKMSLVEHLESTMQQTIFIRLSDKLATKEISEVIEVINHGNSKRKDMVVVVDGMRKMSDDFCEAVQLTLINEAPNVKVMMIFEDLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHNNNENVSDSLPTIKYQYFREAISASCSIGTMMELLKISCQEPSLDQLNIHK 1108
BLAST of AAA family ATPase vs. Planmine SMEST
Match: SMESG000026600.1 (SMESG000026600.1) HSP 1 Score: 682.174 bits (1759), Expect = 0.000e+0 Identity = 335/345 (97.10%), Postives = 341/345 (98.84%), Query Frame = 3
Query: 2208 MMIFEDLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAQFHNNNENVSDSLPTIKY 3242
M+IFEDLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGF+PKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSK+LDHQSQPLHWTDIGGYETVKTM IENIEMPIKHGLEI KGILFHGP GCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELH+VDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKA+F+N NENVSDSLPTIKY
Sbjct: 1 MIIFEDLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFSPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKKLDHQSQPLHWTDIGGYETVKTMSIENIEMPIKHGLEILKGILFHGPHGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHHVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKRKAEFYNYNENVSDSLPTIKY 345 HSP 2 Score: 223.402 bits (568), Expect = 9.844e-65 Identity = 126/273 (46.15%), Postives = 174/273 (63.74%), Query Frame = 3
Query: 855 LNQFTGKDLTDFIDNVYRKFKEITLERL-KACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKP--NVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVR 1664
L+ F+ KD+ F+ + K+ + +L + R+ N S+ L + + +PLHW DIGGYE+VK M IE P+K+G E +KG+LFHGP GC KTMFV ALATE L + ++ PS IMRG VGESE R I +A+ PS++FI++ID++ P R + S V +R++SEL+ FL+GI K NV+ VA TN +D A++R GR+ L + IGLPDQ RESIL I FSD N F ++ N S IAE L+G SGAEIV R+++
Sbjct: 58 LHGFSPKDIRWFLKSASVKYSRTDMRQLIEFSREIEN--TSKKLDHQS--QPLHWTDIGGYETVKTMSIENIEMPIKHGLEILKGILFHGPHGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHHVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLN--FQTEHEIISNISTIAESLTGLSGAEIVGIVREMK 324
BLAST of AAA family ATPase vs. Planmine SMEST
Match: SMESG000026598.1 (SMESG000026598.1) HSP 1 Score: 598.201 bits (1541), Expect = 0.000e+0 Identity = 292/315 (92.70%), Postives = 301/315 (95.56%), Query Frame = 3
Query: 987 YIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRRALPLDTRYTSSGILIRKCHVDSCLSGMAIEGNEISCEFQSISIGSINDNVLKVPIETGNEFNLNRDELFDCEDLVEDISSFLLD 1931
YIADLEP HW DIGGYESVK MLK+EIEEPLKNGKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTI+RGYVGES+MLLRQIFDR VALQPSILFID+IDMIFPSRD L VS VSQR+M+ELLMFL GINEK NVIFVAATNFKDKLD AVMRSGRMGLH EIGLPDQVS ESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIV+FGRQVRLGVRRALPLDTRYTSSGILIRKCHVDSCLSGMAIEGNEISCEFQSISIGSINDNVLKVP ETGN+FN+NRDELFDCEDLVEDI SFLLD
Sbjct: 5 YIADLEPFHWADIGGYESVKSMLKDEIEEPLKNGKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIIRGYVGESKMLLRQIFDRVVALQPSILFIDKIDMIFPSRDPLTVSSVSQRIMTELLMFLGGINEKLNVIFVAATNFKDKLDVAVMRSGRMGLHFEIGLPDQVSPESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVKFGRQVRLGVRRALPLDTRYTSSGILIRKCHVDSCLSGMAIEGNEISCEFQSISIGSINDNVLKVPFETGNKFNINRDELFDCEDLVEDIPSFLLD 319 HSP 2 Score: 219.935 bits (559), Expect = 1.066e-63 Identity = 126/276 (45.65%), Postives = 169/276 (61.23%), Query Frame = 3
Query: 2517 QPLHWTDIGGYETVKTMLIENIEMPIKHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLN--FQTEHEIISNISTIAESLTGLSGAEIVGIVREMK---RKAQFHNNNENVSDSLPTIKYQYFREAISASCSIGTMMELLKISCQEPSL 3329
+P HW DIGGYE+VK+ML + IE P+K+G E KG+LFHGPPGC KTMFV ALATE L + ++ PS I+RG VGES+ R I + + PS++FI+KID++ P R + S+V +RI++EL+ FL GI K NV+ VA TN +D A++R GR+ L IGLPDQ ESIL I FSD N F ++ N S IAE L+G SGAEIV R+++ R+A + S L R+ SC G +E +ISC+ S+
Sbjct: 10 EPFHWADIGGYESVKSMLKDEIEEPLKNGKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIIRGYVGESKMLLRQIFDRVVALQPSILFIDKIDMIFPSRDPLTVSSVSQRIMTELLMFLGGINEK--LNVIFVAATNFKDKLDVAVMRSGRMGLHFEIGLPDQVSPESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVKFGRQVRLGVRRALPLDTRYTSSGIL-------IRKCHVDSCLSGMAIEGNEISCEFQSI 276
BLAST of AAA family ATPase vs. Planmine SMEST
Match: SMESG000027059.1 (SMESG000027059.1) HSP 1 Score: 289.271 bits (739), Expect = 1.077e-85 Identity = 197/539 (36.55%), Postives = 304/539 (56.40%), Query Frame = 3
Query: 126 FKLGQIYLP-FSVNTFSVIKAKDEFYIYEFMKKSKSIKITEIASNIISYSIDFPVFQTAKDCEIEFPIFKNLKYPLFGLEEVENELKIMLNGVG-NSVVVMHGPRGCGKKSLMKRLADEMPNKSFFWINCLTWFNEKDYLKIIYQTL---IANKSIQMVLCCYFINSSNLNEEF-----HLIIDNLVENSQIDIKILLEIPNSDSVDFRRRK----EVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLTDFIDNVYRKFKEITLERLKACRKDSNWIPSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEFGRQVRLGVRRALPLDTR 1700
F LG Y+ S+N +++KA+ +YE + + IKITE S I + + F+ + ++ P K+ K LFGL+ +E +L +LN N +V+++GP GCGK+SL+ L E+ NK F +N ++ LK + L I S +VL + N ++EF H +I++ +E I+I+L + + +S+ + R + FV+ + + E + L TG N E+ S + L FT ++ +F++ V + ++++ L C K+ +IP LL + ++ L W DIGGYESVK ML E+ +KN K+ +KG+LFHGPPGCSKTMFVRALATE+ + L + PS +MR +GESE +L + ++AVA+ PSILFIDEID++ P+ S V+ R+ +L FL N V+FVAATN L+ +++R GR+ I+I LPDQ SRE+IL + FSD+ F DV + IA +LS +SGA+I EFGR+++ + PL R
Sbjct: 33 FSLGDCYIKKLSIN-ITIVKAETNDRVYELIN-NDLIKITEKESGITTEFENSLKFKLDRKAKVLIPETKS-KEKLFGLDAIEQKLFNILNTNKFNRLVILYGPNGCGKRSLINNLELEIENKIFIKLN-------QNKLKERFHYLDKEILGSSAGIVLS---LRLDNFSDEFFISKVHCMIEDYLEEKNTSIQIVLMLKDINSLPPKLRNPRGDKYFVRSLKDEEKAEFLRVL----TGSGNDCAGEFDCLSKS-LQGFTASEIKEFVNEVKQIDPNLSVQTLLECSKNRRYIPKRLLNMVTCVDELRWSDIGGYESVKRMLHEELIHSIKNSKQPIKGILFHGPPGCSKTMFVRALATESNMKLFELTPSVLMRSVLGESEQILAEFINQAVAVAPSILFIDEIDLLLPNEGE-DQSGVTYRLFCQLTTFL---NRSHRVLFVAATNRIGNLNKSLIRPGRIDKLIKIDLPDQQSREAILKLIFSDKK--FTVDADVIASIPMIAGKLSNRSGADIAEFGRKLKKISDKEFPLAIR 547 HSP 2 Score: 220.705 bits (561), Expect = 1.766e-61 Identity = 141/415 (33.98%), Postives = 232/415 (55.90%), Query Frame = 3
Query: 1971 CGKMSLVEHLESTMQQTIFIRLSDKLATKEISEVIEVINHGNSKRKDMVVVVDGMRKMSDDF------CEAVQLTLINEAPNVKVMMIFEDLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSS--VEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDHQS---QPLHWTDIGGYETVKTMLIENIEMPIKHGLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVREMKR 3182
CGK SL+ +LE ++ IFI KL ++ E ++ +V+ + SD+F C ++ L + +++++++ +D+ +P +L K + KD E K ++L + + + L K+L GFT +I+ F+ ++ L+E S+ K L + L W+DIGGYE+VK ML E + IK+ + KGILFHGPPGC KTMFV ALATE +K+F + PS +MR ++GESE ++QA+ +PS++FI++IDLL+P + +S V R+ +L TFL+ ++ V+ VA TN + N++++L+RPGR+ ++ I LPDQ+ RE+ILK+ FSD F + ++I++I IA L+ SGA+I R++K+
Sbjct: 136 CGKRSLINNLELEIENKIFI----KLNQNKLKERFHYLDKEILGSSAGIVLSLRLDNFSDEFFISKVHC-MIEDYLEEKNTSIQIVLMLKDINSLPPKLRNPRGDKYFVRSLKDEE---KAEFLRVLTGSGNDCAGEFDCLSKSLQGFTASEIKEFVNEVKQIDPNLSVQTLLECSKNRRYIPKRLLNMVTCVDELRWSDIGGYESVKRMLHEELIHSIKNSKQPIKGILFHGPPGCSKTMFVRALATESNMKLFELTPSVLMRSVLGESEQILAEFINQAVAVAPSILFIDEIDLLLPNEGED-QSGVTYRLFCQLTTFLN-----RSHRVLFVAATNRIGNLNKSLIRPGRIDKLIKIDLPDQQSREAILKLIFSDKKFTVDADVIASIPMIAGKLSNRSGADIAEFGRKLKK 536
BLAST of AAA family ATPase vs. Planmine SMEST
Match: SMESG000009924.1 (SMESG000009924.1) HSP 1 Score: 155.606 bits (392), Expect = 2.210e-38 Identity = 136/482 (28.22%), Postives = 220/482 (45.64%), Query Frame = 3
Query: 414 VVMHGPRGCGKKSLMKRLADEMPNKSFFWINC------LTWFNEKDYLKIIYQTLIANKSIQMVLCCYFINS-SNLNEEFH------------LIIDNLVENSQIDIKILLEIPNSDSVDFRR----RKEVFVKIIDKNSRFELFKYLINRSTGMDNFDDPEWKKFSDNQLNQFTGKDLT---------------DFIDNVYRKFKEITLERLKACRKDSNWI-----PSELLAYIADLEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEFVK-------GVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSR--DHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIVEF-GRQVRLGVRRALPLDTR 1700
++++GP G GK + + +A+E FF IN L +E + K + A K+ ++ +++ + E+ H ++D L + S + + PNS RR +EV + I D R E+ + + M DD + ++ + ++ + G DL D ID + L L +D W PS L ++ + W DIGG + VK L+ ++ P+++ +F+K GVLF+GPPGC KT+ +A+A E N ISIK ++ + GESE +R IFD+A P +LF DE+D I SR + R++++LL ++G++ K NV + ATN D +DAA++R GR+ I I LPD+ SR IL A DV N+ +A+ G SGA++ E R + +R + D R
Sbjct: 238 ILLYGPPGTGKTLIARAVANET-GSFFFLINGPEIMSKLAGESESNLRKAFEE---AEKNSPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRSHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDTTGRLEILRI---HTKNMRLGDDVDLEQVA-SETHGHVGADLAALCTEAALQQIRNKMDLIDLEEPEIDAEILNSLAVTMEDFRWALSKSNPSALRETTVEVPNVTWEDIGGLQGVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDIFDKARQAAPCVLFFDELDSIAKSRGGSVGDGGGAADRVINQLLTEMDGMSAKKNVFIIGATNRPDIIDAAILRPGRLDQLIYIPLPDEKSRIQILKANLRKSP----IAKDVDVNY--MAKVTHGFSGADLTEICQRACKQAIRECIEADIR 705 HSP 2 Score: 146.747 bits (369), Expect = 1.305e-35 Identity = 109/373 (29.22%), Postives = 178/373 (47.72%), Query Frame = 3
Query: 2223 DLKLVPSQLDGFVNKKVCMSLPKDTEECLKHMIYKILNIKSSVEDIEELVKNLHGFTPKDIRWFLKSASVKYSRTDMRQLIEFSREIENTSKELDHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKR--SSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGI--------VRE-------MKRKAQFHNNN--ENVSDSLPTIKYQYFREAI 3263
DL+ V S+ G V + TE L+ + K+ I +I+ + N T +D RW L S+++ L E + E+ N + W DIGG + VK L E ++ P++H G+ KG+LF+GPPGC KT+ A+A ECQ ++ +++ GESEA R I +A +++P ++F +++D + R S +R++++L+T + G+ KK NV I+ TN +D A+LRPGRL ++ I LPD+K R ILK + +++ +A+ G SGA++ I +RE R Q E+ D +P I ++F EA+
Sbjct: 392 DLEQVASETHGHVGADLAALC---TEAALQQIRNKMDLIDLEEPEIDAEILNSLAVTMEDFRWAL-------SKSNPSALRETTVEVPNVT-----------WEDIGGLQGVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDIFDKARQAAPCVLFFDELDSIAKSRGGSVGDGGGAADRVINQLLTEMDGMSAKK--NVFIIGATNRPDIIDAAILRPGRLDQLIYIPLPDEKSRIQILKANLRKSPIAKD----VDVNYMAKVTHGFSGADLTEICQRACKQAIRECIEADIRAARDQQLKGGEPMEDDIDPVPEITRKHFEEAM 737 HSP 3 Score: 127.102 bits (318), Expect = 1.762e-29 Identity = 78/222 (35.14%), Postives = 118/222 (53.15%), Query Frame = 3
Query: 999 LEPLHWVDIGGYESVKVMLKNEIEEPLKNGKEF-------VKGVLFHGPPGCSKTMFVRALATETGLNLISIKPSTIMRGYVGESEMLLRQIFDRAVALQPSILFIDEIDMIFPSRDHLAVSPVSQRMMSELLMFLNGINEKPNVIFVAATNFKDKLDAAVMRSGRMGLHIEIGLPDQVSRESILGITFSDQNDGFIFADDVKENFSAIAERLSGKSGAEIV 1643
L + + DIGG +K +E PL++ + F +G+L +GPPG KT+ RA+A ETG I IM GESE LR+ F+ A P+I+FIDE+D I P R+ V +R++S+LL ++G+ ++ +VI +AATN + +D A+ R GR ++IG+PD R IL I + G DDV + +A G GA++
Sbjct: 195 LNEVGYDDIGGCRKQLAQIKEMVELPLRHPQLFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGSFFFLINGPEIMSKLAGESESNLRKAFEEAEKNSPAIIFIDELDAIAPKREKTH-GEVERRIVSQLLTLMDGLKQRSHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDTTGRLEILRIHTKNMRLG----DDV--DLEQVASETHGHVGADLA 409 HSP 4 Score: 126.716 bits (317), Expect = 2.304e-29 Identity = 73/230 (31.74%), Postives = 125/230 (54.35%), Query Frame = 3
Query: 2505 DHQSQPLHWTDIGGYETVKTMLIENIEMPIKH-------GLEIPKGILFHGPPGCPKTMFVNALATECQLKMFNVGPSDIMRGIVGESEAAFRAILSQALESSPSLIFIEKIDLLIPKRSSNSKSNVCERILSELMTFLSGIETKKNQNVMIVATTNELHNVDEALLRPGRLSLIVPIGLPDQKDRESILKIGFSDLNFQTEHEIISNISTIAESLTGLSGAEIVGIVRE 3173
D + + + DIGG + E +E+P++H G++ P+GIL +GPPG KT+ A+A E F + +IM + GESE+ R +A ++SP++IFI+++D + PKR + V RI+S+L+T + G+ K+ +V+++A TN +++D AL R GR V IG+PD R IL+I ++ + ++ +A G GA++ + E
Sbjct: 192 DEKLNEVGYDDIGGCRKQLAQIKEMVELPLRHPQLFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGSFFFLINGPEIMSKLAGESESNLRKAFEEAEKNSPAIIFIDELDAIAPKREK-THGEVERRIVSQLLTLMDGL--KQRSHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDTTGRLEILRIHTKNMRLGDD----VDLEQVASETHGHVGADLAALCTE 414 The following BLAST results are available for this feature:
BLAST of AAA family ATPase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of AAA family ATPase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of AAA family ATPase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of AAA family ATPase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of AAA family ATPase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of AAA family ATPase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of AAA family ATPase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of AAA family ATPase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of AAA family ATPase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of AAA family ATPase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of AAA family ATPase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of AAA family ATPase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of AAA family ATPase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of AAA family ATPase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30034256 ID=SMED30034256|Name=AAA family ATPase|organism=Schmidtea mediterranea sexual|type=transcript|length=3411bpback to top protein sequence of SMED30034256-orf-1 >SMED30034256-orf-1 ID=SMED30034256-orf-1|Name=SMED30034256-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1109bp MNISVVDYIGLELNHLKVNLFEKSAITQFLQNPFKLGQIYLPFSVNTFSVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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