Riboflavin synthase
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppAnatomical Expression
PAGE et. al., 2020SMED30034182 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of Riboflavin synthase vs. UniProt/SwissProt
Match: sp|O67604|RISA_AQUAE (Riboflavin synthase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=ribE PE=3 SV=1) HSP 1 Score: 143.665 bits (361), Expect = 1.678e-41 Identity = 87/206 (42.23%), Postives = 127/206 (61.65%), Query Frame = 1 Query: 37 MFTGIISAIGEIVALASLFEPSSLSDEQYFGKKLTIRTPLGYLDDVKIGDSIALNGACMTVVKIHEDALCFDADISHESLRLT--AGLERCGLVNLEKALRLGASLDGHLVTGHVDGTAQVLAFEPVGESRMLCVLIPKSFAKFCAIKGSVTLAGVSLTVNQVKDSDAGCEVTVNLIPHTCEKTLLKNLKRDDFVNFEIDPLARYC 648 MFTG++ +G++ L +LS + G KL++ T L +DVK+GDS+++NGAC+TVV I L FD +S E+L+ T L+ VNLE+ALR+G L GH+V GHVD TA V +F +GE L + IP+ ++ + KGS+ L G+SLTVN VK++ +V +N+IPHT + T L+ K D +N E D L +Y Sbjct: 1 MFTGLVEDLGKVKNL-------TLSSK---GAKLSVETKL---EDVKLGDSVSVNGACLTVVDIKSSTLTFD--VSPETLKRTNLGKLKTGDYVNLERALRVGERLGGHIVQGHVDFTAPVKSFNFLGEHYELVIEIPEEWSIYVVEKGSIALDGISLTVNYVKEN----KVFINIIPHTYKSTNLQFKKVGDLLNVETDILGKYV 187
BLAST of Riboflavin synthase vs. UniProt/SwissProt
Match: sp|P51961|RISA_PHOPO (Riboflavin synthase OS=Photobacterium phosphoreum OX=659 GN=ribE PE=1 SV=1) HSP 1 Score: 131.339 bits (329), Expect = 1.402e-36 Identity = 87/215 (40.47%), Postives = 119/215 (55.35%), Query Frame = 1 Query: 37 MFTGIISAIGEIVALASLFEPSSLSDEQYFGKKLTIRTPLGYLD--DVKIGDSIALNGACMTVVKIHEDALCFDADISHESLRLTA-GLERCG-LVNLEKALRLGASLDGHLVTGHVDGTAQVLAFEPVGESRMLCVLIPKSFAKFCAIKGSVTLAGVSLTVNQVKDSDAGCEVTVNLIPHTCEKTLLKNLKRDDFVNFEIDPLARYCARITEVD 669 MFTGII A+G I A+ S G + LD DVKIGDSIA NG C+TVVK+ ++ + AD+S E+L TA + G VNLEKA+ GH+V+GHVD A+V+ G + + + +P K+ + KGSVT+ GVSLTVN V G E + ++PHT +T + + K + VN E+D LARY R+ VD Sbjct: 1 MFTGIIEAVGNISAITSK------------GSDFEVSVNCDTLDLADVKIGDSIATNGICLTVVKLTANS--YVADLSIETLSRTAFNYYKVGQAVNLEKAMLPTTRFGGHIVSGHVDAVAEVIECRTSGRAIDIWIRVPSQIEKYLSEKGSVTVDGVSLTVNAV----TGNEFKLTIVPHTVVETTIADFKVGNKVNIEVDVLARYIERLLLVD 197
BLAST of Riboflavin synthase vs. UniProt/SwissProt
Match: sp|Q01993|RISA_PHOLE (Riboflavin synthase OS=Photobacterium leiognathi OX=553611 GN=ribE PE=3 SV=1) HSP 1 Score: 130.954 bits (328), Expect = 1.814e-36 Identity = 85/213 (39.91%), Postives = 120/213 (56.34%), Query Frame = 1 Query: 37 MFTGIISAIGEIVALASLFEPSSLSDEQYFGKKLTIRTPLGYLDDVKIGDSIALNGACMTVVKIHEDALCFDADISHESLRLTA-GLERCGL-VNLEKALRLGASLDGHLVTGHVDGTAQVLAFEPVGESRMLCVLIPKSFAKFCAIKGSVTLAGVSLTVNQVKDSDAGCEVTVNLIPHTCEKTLLKNLKRDDFVNFEIDPLARYCARITEVD 669 MFTGII +IG I A+ E S+ + T + DV IGDSIA NG C+TV K+ + AD+S E+ + TA R G VNLEKA+ L GHLV+GHVDG +V+ F+ G + + V +P K+ + KGSVT+ G+SLT+N V + + + ++PHT +T L N+ D VN EID +ARY ++ +VD Sbjct: 1 MFTGIIESIGNIGAIIRHNEDLSI----------VVNTNNLDISDVNIGDSIATNGVCLTVSKLLPSG--YTADLSLETYKRTAFHSYRIGQEVNLEKAMLPTTRLGGHLVSGHVDGVGEVIEFKRNGRAINIWVAVPVQLKKYLSEKGSVTIDGISLTINAVYQN----VIKLTIVPHTLAETNLVNINIDKKVNVEIDMMARYLEKLIKVD 197
BLAST of Riboflavin synthase vs. UniProt/SwissProt
Match: sp|Q2YN92|RISA_BRUA2 (Riboflavin synthase OS=Brucella abortus (strain 2308) OX=359391 GN=ribE PE=1 SV=1) HSP 1 Score: 124.02 bits (310), Expect = 5.089e-34 Identity = 82/214 (38.32%), Postives = 117/214 (54.67%), Query Frame = 1 Query: 37 MFTGIISAIGEIVALASLFEPSSLSDEQYFGKKLTIRTPLGYLDDVKIGDSIALNGACMTVVKIHE---DALCFDADISHESLRLT--AGLERCGLVNLEKALRLGASLDGHLVTGHVDGTAQVLAFEPVGESRMLCVLIPKSFAKFCAIKGSVTLAGVSLTVNQVKDSDAGCEVTVNLIPHTCEKTLLKNLKRDDFVNFEIDPLARYCARITE 663 MFTGII+ IG++ + L E L E + + +++G SIA +G C+TVV + E +A F+ + E+LRLT + + +NLE++L+LG + GHLV GHVDG A+++ + G++ + P+ A F A KGSV L G SLTVN V + E V LI H+ E T K D VN EID LARY AR+ + Sbjct: 1 MFTGIITDIGKVDRVKPLNEGVLLRIETAYDP-----------ETIELGASIACSGVCLTVVALPEKGSNARWFEVEAWEEALRLTTISSWQSGRKINLERSLKLGDEMGGHLVFGHVDGQAEIVERKDEGDAVRFTLRAPEELAPFIAQKGSVALDGTSLTVNGVNAN----EFDVLLIRHSLEVTTWGERKAGDKVNIEIDQLARYAARLAQ 199
BLAST of Riboflavin synthase vs. UniProt/SwissProt
Match: sp|P9WK35|RISA_MYCTU (Riboflavin synthase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ribE PE=1 SV=1) HSP 1 Score: 118.627 bits (296), Expect = 8.067e-32 Identity = 83/213 (38.97%), Postives = 116/213 (54.46%), Query Frame = 1 Query: 37 MFTGIISAIGEIVALASLFEPSSLSDEQYFGKKLTIRTPLGYLDDVKIGDSIALNGACMTVVKIHEDALCFDADISHESL-RLTAGLERCG-LVNLEKALRLGASLDGHLVTGHVDGTAQVLAFEPVGESRMLCVLIPKSFAKFCAIKGSVTLAGVSLTVNQVKDSDAGCE----VTVNLIPHTCEKTLLKNLKRDDFVNFEIDPLARYCARI 657 MFTGI+ GE+ +L + + +LTIR P+ D GDSIA+NG C+TVV + D F AD+ E+L R G R G VNLE+A LG+ L GH+V GHVD T +++A P ++ + +P S A++ KGS+T+ G+SLTV S G E V+LIP T E T L + VN E+D +A+Y R+ Sbjct: 1 MFTGIVEERGEVTGREALVDAA----------RLTIRGPM-VTADAGHGDSIAVNGVCLTVVDVLPDG-QFTADVMAETLNRSNLGELRPGSRVNLERAAALGSRLGGHIVQGHVDATGEIVARCPSEHWEVVRIEMPASVARYVVEKGSITVDGISLTV-----SGLGAEQRDWFEVSLIPTTRELTTLGSAAVGTRVNLEVDVVAKYVERL 196
BLAST of Riboflavin synthase vs. TrEMBL
Match: A0A3M6Q398 (Riboflavin synthase OS=Comamonadaceae bacterium NML970147 OX=1076744 GN=EBQ26_08490 PE=4 SV=1) HSP 1 Score: 222.246 bits (565), Expect = 7.990e-70 Identity = 117/209 (55.98%), Postives = 140/209 (66.99%), Query Frame = 1 Query: 37 MFTGIISAIGEIVALASLFEPSSLSDEQYFGKKLTIRTPLGYLDDVKIGDSIALNGACMTVVKIHEDALCFDADISHESLRLTAGLERCGLVNLEKALRLGASLDGHLVTGHVDGTAQVLAFEPVGESRMLCVLIPKSFAKFCAIKGSVTLAGVSLTVNQVKDSDAGCEVTVNLIPHTCEKTLLKNLKRDDFVNFEIDPLARYCARITE 663 MFTGII+AIG I A+ +L D GK+L I TP GYLDDV +GDSIALNGACMTV A F +IS ESLRLTAGL++ G VNLEKALR L GH+V+GHVDG QV F PVGES L +L P++ A F A KGS+T+ GVSLTVN V+D GCE ++NLI HT T L+ L+ VN E+D +ARY AR+ E Sbjct: 1 MFTGIITAIGHIRAV------HALGDSAAHGKRLDITTPAGYLDDVALGDSIALNGACMTVTAFDAAAAQFSIEISAESLRLTAGLDQPGPVNLEKALRAADRLGGHIVSGHVDGIGQVSHFAPVGESHELRILAPQALAPFLAYKGSITVQGVSLTVNAVRDVPQGCEFSINLIAHTLAHTTLQYLQTGSRVNLEVDLMARYVARMLE 203
BLAST of Riboflavin synthase vs. TrEMBL
Match: A0A3M6R7P3 (Riboflavin synthase OS=Comamonadaceae bacterium NML180581 OX=2478745 GN=EBQ24_01370 PE=4 SV=1) HSP 1 Score: 222.246 bits (565), Expect = 7.990e-70 Identity = 117/209 (55.98%), Postives = 140/209 (66.99%), Query Frame = 1 Query: 37 MFTGIISAIGEIVALASLFEPSSLSDEQYFGKKLTIRTPLGYLDDVKIGDSIALNGACMTVVKIHEDALCFDADISHESLRLTAGLERCGLVNLEKALRLGASLDGHLVTGHVDGTAQVLAFEPVGESRMLCVLIPKSFAKFCAIKGSVTLAGVSLTVNQVKDSDAGCEVTVNLIPHTCEKTLLKNLKRDDFVNFEIDPLARYCARITE 663 MFTGII+AIG I A+ +L D GK+L I TP GYLDDV +GDSIALNGACMTV A F +IS ESLRLTAGL++ G VNLEKALR L GH+V+GHVDG QV F PVGES L +L P++ A F A KGS+T+ GVSLTVN V+D GCE ++NLI HT T L+ L+ VN E+D +ARY AR+ E Sbjct: 1 MFTGIITAIGHIRAV------HALGDSAAHGKRLDITTPAGYLDDVALGDSIALNGACMTVTAFDAAAAQFSIEISAESLRLTAGLDQPGPVNLEKALRAADRLGGHIVSGHVDGIGQVSHFAPVGESHELRILAPQALAPFLAYKGSITVQGVSLTVNAVRDVPQGCEFSINLIAHTLAHTTLQYLQTGSRVNLEVDLMARYVARMLE 203
BLAST of Riboflavin synthase vs. TrEMBL
Match: A0A3P1WEV0 (Riboflavin synthase OS=Comamonadaceae bacterium OH3737_COT-264 OX=2491055 GN=EII18_01360 PE=4 SV=1) HSP 1 Score: 222.246 bits (565), Expect = 7.990e-70 Identity = 117/209 (55.98%), Postives = 140/209 (66.99%), Query Frame = 1 Query: 37 MFTGIISAIGEIVALASLFEPSSLSDEQYFGKKLTIRTPLGYLDDVKIGDSIALNGACMTVVKIHEDALCFDADISHESLRLTAGLERCGLVNLEKALRLGASLDGHLVTGHVDGTAQVLAFEPVGESRMLCVLIPKSFAKFCAIKGSVTLAGVSLTVNQVKDSDAGCEVTVNLIPHTCEKTLLKNLKRDDFVNFEIDPLARYCARITE 663 MFTGII+AIG I A+ +L D GK+L I TP GYLDDV +GDSIALNGACMTV A F +IS ESLRLTAGL++ G VNLEKALR L GH+V+GHVDG QV F PVGES L +L P++ A F A KGS+T+ GVSLTVN V+D GCE ++NLI HT T L+ L+ VN E+D +ARY AR+ E Sbjct: 1 MFTGIITAIGHIRAV------HALGDSAAHGKRLDITTPAGYLDDVALGDSIALNGACMTVTAFDAAAAQFSIEISAESLRLTAGLDQPGPVNLEKALRAADRLGGHIVSGHVDGIGQVSHFAPVGESHELRILAPQALAPFLAYKGSITVQGVSLTVNAVRDVPQGCEFSINLIAHTLAHTTLQYLQTGSRVNLEVDLMARYVARMLE 203
BLAST of Riboflavin synthase vs. TrEMBL
Match: A0A3M6QI31 (Riboflavin synthase OS=Comamonadaceae bacterium NML161473 OX=2478744 GN=EBQ25_00340 PE=4 SV=1) HSP 1 Score: 222.246 bits (565), Expect = 7.990e-70 Identity = 117/209 (55.98%), Postives = 140/209 (66.99%), Query Frame = 1 Query: 37 MFTGIISAIGEIVALASLFEPSSLSDEQYFGKKLTIRTPLGYLDDVKIGDSIALNGACMTVVKIHEDALCFDADISHESLRLTAGLERCGLVNLEKALRLGASLDGHLVTGHVDGTAQVLAFEPVGESRMLCVLIPKSFAKFCAIKGSVTLAGVSLTVNQVKDSDAGCEVTVNLIPHTCEKTLLKNLKRDDFVNFEIDPLARYCARITE 663 MFTGII+AIG I A+ +L D GK+L I TP GYLDDV +GDSIALNGACMTV A F +IS ESLRLTAGL++ G VNLEKALR L GH+V+GHVDG QV F PVGES L +L P++ A F A KGS+T+ GVSLTVN V+D GCE ++NLI HT T L+ L+ VN E+D +ARY AR+ E Sbjct: 1 MFTGIITAIGHIRAV------HALGDSAAHGKRLDITTPAGYLDDVALGDSIALNGACMTVTAFDAAAAQFSIEISAESLRLTAGLDQPGPVNLEKALRAADRLGGHIVSGHVDGIGQVSHFAPVGESHELRILAPQALAPFLAYKGSITVQGVSLTVNAVRDVPQGCEFSINLIAHTLAHTTLQYLQTGSRVNLEVDLMARYVARMLE 203
BLAST of Riboflavin synthase vs. TrEMBL
Match: A0A522BL60 (Riboflavin synthase OS=Aquabacterium sp. OX=1872578 GN=EPO01_16035 PE=4 SV=1) HSP 1 Score: 221.861 bits (564), Expect = 1.029e-69 Identity = 115/207 (55.56%), Postives = 141/207 (68.12%), Query Frame = 1 Query: 37 MFTGIISAIGEIVALASLFEPSSLSDEQYFGKKLTIRTPLGYLDDVKIGDSIALNGACMTVVKIHEDALCFDADISHESLRLTAGLERCGLVNLEKALRLGASLDGHLVTGHVDGTAQVLAFEPVGESRMLCVLIPKSFAKFCAIKGSVTLAGVSLTVNQVKDSDAGCEVTVNLIPHTCEKTLLKNLKRDDFVNFEIDPLARYCARI 657 MFTGII++IG+I E SL + GK+LTI+ P GYLDDV++GDSIAL GACMTV + +D L F DIS ESLRLT GL+ G VNLEKALR L GH+V+GHVDG +V +FEPVGES L + P+ A+F A KGS+T+ GVSLTVN+V D AG ++NLIPHT + T L LK VN EID +ARY R+ Sbjct: 1 MFTGIITSIGQIT------EVQSLGADPSHGKRLTIQAPAGYLDDVELGDSIALQGACMTVTTLSDDRLRFTIDISAESLRLTRGLDSPGAVNLEKALRAHDRLGGHIVSGHVDGLGEVTSFEPVGESWELRIRAPRDLARFLAYKGSITVNGVSLTVNKVADEAAGVNFSINLIPHTLQHTTLGQLKAGSPVNLEIDLIARYVERM 201
BLAST of Riboflavin synthase vs. Ensembl Yeast
Match: RIB5 (Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway [Source:SGD;Acc:S000000460]) HSP 1 Score: 92.4337 bits (228), Expect = 2.079e-23 Identity = 64/201 (31.84%), Postives = 101/201 (50.25%), Query Frame = 1 Query: 37 MFTGIISAIGEIVALASLFEPSSLSDEQYFGKKLTIRTPLGYLDDVKIGDSIALNGACMTVVKIHEDALCFDADISHESLRLT--AGLERCGLVNLEKALRLGASLDGHLVTGHVDGTAQVLAFEPVGESRMLCV-LIPKSFAKFCAIKGSVTLAGVSLTVNQVKDSDAGCEVTVNLIPHTCEKTLLKNLKRDDFVNFEID 630 MFTGI+ +G ++ P S+ G +TI L D +GDSIA+NG C+TV + + D+ F IS E+++ + A + VNLE+A+ GH V GHVD A +++ P G S + L + + K+ KG + + G SLT+ +V G +++I HT + ++ K D VN E+D Sbjct: 1 MFTGIVECMGTVLE----NNPYDDSESGGQGVSITIGNAGSILTDCHVGDSIAVNGVCLTVTEFNNDS--FKVGISPETIKRSNVASWIQGTQVNLERAVSQDVRFGGHYVQGHVDTVANIVSRRPEGNSIIFGFQLRDQEYFKYIVEKGFICIDGTSLTIIKVDPLSQGGAFYISMIKHTQDNVIMPLKKIGDEVNIEVD 195 The following BLAST results are available for this feature:
BLAST of Riboflavin synthase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Riboflavin synthase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Riboflavin synthase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Riboflavin synthase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Riboflavin synthase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Riboflavin synthase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Riboflavin synthase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Riboflavin synthase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Riboflavin synthase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Riboflavin synthase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Riboflavin synthase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Riboflavin synthase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Riboflavin synthase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Riboflavin synthase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 0
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30034182 ID=SMED30034182|Name=Riboflavin synthase|organism=Schmidtea mediterranea sexual|type=transcript|length=696bpback to top protein sequence of SMED30034182-orf-1 >SMED30034182-orf-1 ID=SMED30034182-orf-1|Name=SMED30034182-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=232bp MRASELWQKKESMFTGIISAIGEIVALASLFEPSSLSDEQYFGKKLTIRTback to top Annotated Terms
The following terms have been associated with this transcript:
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