Calcium-dependent protein kinase

Overview
NameCalcium-dependent protein kinase
Smed IDSMED30034173
Length (bp)1034
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Calcium-dependent protein kinase (SMED30034173) t-SNE clustered cells

Violin plots show distribution of expression levels for Calcium-dependent protein kinase (SMED30034173) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Calcium-dependent protein kinase (SMED30034173) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Calcium-dependent protein kinase (SMED30034173) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30034173

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30034173SMESG000033648.1 SmedASXL_013578SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30034173SMESG000033648.1 dd_Smed_v4_13677_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30034173SMESG000033648.1 dd_Smed_v4_13677_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30034173SMESG000033648.1 dd_Smed_v4_13677_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30034173SMESG000033648.1 dd_Smed_v4_13677_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30034173SMESG000033648.1 dd_Smed_v4_13677_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30034173SMESG000033648.1 dd_Smed_v6_13677_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30034173SMESG000033648.1 dd_Smed_v4_13677_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Human
Match: DST (dystonin [Source:HGNC Symbol;Acc:HGNC:1090])

HSP 1 Score: 51.6026 bits (122), Expect = 1.315e-6
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct:  215 NDALDRLEELREFANFD---FDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 306
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Human
Match: DST (dystonin [Source:HGNC Symbol;Acc:HGNC:1090])

HSP 1 Score: 51.2174 bits (121), Expect = 2.422e-6
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct:  950 NDALDRLEELREFANFD---FDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 1041
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Human
Match: MACF1 (microtubule actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664])

HSP 1 Score: 50.447 bits (119), Expect = 2.812e-6
Identity = 26/95 (27.37%), Postives = 51/95 (53.68%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P   +E+  +A + D DG+  ID+ EF
Sbjct:   35 NDALDRLEELKEFANFD---FDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTTKLEMTAVADIFDRDGDGYIDYYEF 126
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Human
Match: DST (dystonin [Source:HGNC Symbol;Acc:HGNC:1090])

HSP 1 Score: 50.447 bits (119), Expect = 4.757e-6
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 2273 NDALDRLEELREFANFD---FDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 2364
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Human
Match: DST (dystonin [Source:HGNC Symbol;Acc:HGNC:1090])

HSP 1 Score: 49.6766 bits (117), Expect = 7.895e-6
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 5081 NDALDRLEELREFANFD---FDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 5172
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Zebrafish
Match: DST (dystonin [Source:HGNC Symbol;Acc:HGNC:1090])

HSP 1 Score: 51.6026 bits (122), Expect = 1.168e-6
Identity = 27/95 (28.42%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + +R  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 3377 NDALDRLEELKEFANFD---FDVWRKRYMRWMNHKKSRVMDFFRRIDKDQDGKVTRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 3468
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Zebrafish
Match: macf1b (microtubule actin crosslinking factor 1b [Source:ZFIN;Acc:ZDB-GENE-100922-36])

HSP 1 Score: 49.2914 bits (116), Expect = 2.106e-6
Identity = 34/151 (22.52%), Postives = 68/151 (45.03%), Query Frame = 3 
Query:  297 ERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIRYFRHMESLKHGIVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKE 749
            +R  +W      +IL++F  +D D+ G +T+++F   ++    P N++E+  +A + D +G+  ID+ EF   +   R               PYR T  I ++ E    +   CN     F+  +     +++    D+  L M+ I++ 
Sbjct:   15 KRYMQWISHLKSRILDVFRSIDRDQDGRITHKEFIDSVLASKFPTNVLEMTAVANIFDVNGDGFIDYYEFVSALHPSR--------------DPYRRTVDIDQINEEVSRQVSQCNCP-KRFQVEQISANRYRF---GDSQQLRMVRILRS 147
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Xenopus
Match: rab11fip2 (RAB11 family interacting protein 2 (class I) [Source:Xenbase;Acc:XB-GENE-5947669])

HSP 1 Score: 50.447 bits (119), Expect = 4.071e-6
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 5964 NDALDRLEELREFANFD---FDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIEGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 6055
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Xenopus
Match: rab11fip2 (RAB11 family interacting protein 2 (class I) [Source:Xenbase;Acc:XB-GENE-5947669])

HSP 1 Score: 50.447 bits (119), Expect = 4.139e-6
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 5763 NDALDRLEELREFANFD---FDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIEGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 5854
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Xenopus
Match: rab11fip2 (RAB11 family interacting protein 2 (class I) [Source:Xenbase;Acc:XB-GENE-5947669])

HSP 1 Score: 50.447 bits (119), Expect = 4.179e-6
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 5916 NDALDRLEELREFANFD---FDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIEGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 6007
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Xenopus
Match: rab11fip2 (RAB11 family interacting protein 2 (class I) [Source:Xenbase;Acc:XB-GENE-5947669])

HSP 1 Score: 50.447 bits (119), Expect = 4.292e-6
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 6175 NDALDRLEELREFANFD---FDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIEGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 6266
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Xenopus
Match: rab11fip2 (RAB11 family interacting protein 2 (class I) [Source:Xenbase;Acc:XB-GENE-5947669])

HSP 1 Score: 50.0618 bits (118), Expect = 4.989e-6
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 6534 NDALDRLEELREFANFD---FDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIEGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 6625
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Mouse
Match: Dst (dystonin [Source:MGI Symbol;Acc:MGI:104627])

HSP 1 Score: 51.2174 bits (121), Expect = 6.705e-7
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct:    2 NDALDRLEELREFANFD---FDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 93
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Mouse
Match: Macf1 (microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559])

HSP 1 Score: 50.447 bits (119), Expect = 1.957e-6
Identity = 26/95 (27.37%), Postives = 51/95 (53.68%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P   +E+  +A + D DG+  ID+ EF
Sbjct:   35 NDALDRLEELKEFANFD---FDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTTKLEMTAVADIFDRDGDGYIDYYEF 126
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Mouse
Match: Dst (dystonin [Source:MGI Symbol;Acc:MGI:104627])

HSP 1 Score: 50.8322 bits (120), Expect = 2.220e-6
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 1221 NDALDRLEELREFANFD---FDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 1312
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Mouse
Match: Dst (dystonin [Source:MGI Symbol;Acc:MGI:104627])

HSP 1 Score: 50.447 bits (119), Expect = 3.352e-6
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 7276 NDALDRLEELREFANFD---FDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 7367
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Mouse
Match: Dst (dystonin [Source:MGI Symbol;Acc:MGI:104627])

HSP 1 Score: 50.447 bits (119), Expect = 3.407e-6
Identity = 26/95 (27.37%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 6989 NDALDRLEELREFANFD---FDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 7080
[Close]
BLAST of Calcium-dependent protein kinase vs. UniProt/SwissProt
Match: sp|P28582|CDPK_DAUCA (Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 3.368e-6
Identity = 26/77 (33.77%), Postives = 43/77 (55.84%), Query Frame = 3 
Query:  321 ENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIRYFRHMESLKH 551
            E +K + ++F  +D+DK+G +TYE+ K+GL       + +E+  L    D DGN  ID+ EF     +   +ES +H
Sbjct:  382 EEIKGLKSMFANMDTDKSGTITYEELKSGLARLGSKLSEVEVQQLMDAADVDGNGTIDYLEFITATMHRHKLESYEH 458
[Close]
BLAST of Calcium-dependent protein kinase vs. TrEMBL
Match: A0A1I8I5H9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001410g3 PE=4 SV=1)

HSP 1 Score: 184.882 bits (468), Expect = 8.033e-53
Identity = 92/226 (40.71%), Postives = 146/226 (64.60%), Query Frame = 3 
Query:  225 RALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIRYFRHMESLKHGIVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDF 902
            RA   L K++E YK DQ++Y +FV R+D WF +N K +L +F + D D  G L Y++FKAG++D NIPCNLIELHLL TLLD DGN  ID++EF  GI++F+ +ES   GI+  ES+P       +K++   + ++++   +FL FE    HPGHF+ ++P +T+   ++  I++  +  +    +F +N+K+ +  L E KTL++ G  GG  + P++ ++FYDF
Sbjct:   24 RAQAQLAKMMEGYKADQQRYKEFVNRVDNWFTQNCKVMLELFTKFDRDGNGTLDYDEFKAGMLDMNIPCNLIELHLLCTLLDADGNGDIDYDEFGNGIKHFKEVES--GGIL--ESAP-------LKIVGPREQRFVSVLFRFLIFEQQTNHPGHFEQVVPLETHGHTLVEFIRKRTKLASKDFVLFFDNAKTNQ--LDEYKTLEDHGIHGGPEHAPKQTLVFYDF 236
[Close]
BLAST of Calcium-dependent protein kinase vs. TrEMBL
Match: A0A267FHL4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001410g2 PE=4 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 1.918e-52
Identity = 90/226 (39.82%), Postives = 144/226 (63.72%), Query Frame = 3 
Query:  225 RALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIRYFRHMESLKHGIVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDF 902
            RA   L K++E YK DQ++Y +FV R+D WF +N K +L +F + D D  G L Y++FKAG++D NIPCNLIELHLL TLLD DGN  ID++EF  GI++F++ + L     E ES    E+   +K++   + ++++   +FL FE    HPGHF+ ++P +T+   ++  I++  +  +    +F +N+K+ +  L E KTL++ G  GG  + P++ ++FYDF
Sbjct:   24 RAQAQLAKMMEGYKADQQRYKEFVNRVDNWFTQNCKVMLELFTKFDRDGNGTLDYDEFKAGMLDMNIPCNLIELHLLCTLLDADGNGDIDYDEFGNGIKHFKNFKGLLRQRREVESGGILESAP-LKIVGPREQRFVSVLFRFLIFEQQTNHPGHFEQVVPLETHGHTLVEFIRKRTKLASKDFVLFFDNAKTNQ--LDEYKTLEDHGIHGGPEHAPKQTLVFYDF 246
[Close]
BLAST of Calcium-dependent protein kinase vs. TrEMBL
Match: A0A1I8I381 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 2.428e-51
Identity = 92/226 (40.71%), Postives = 146/226 (64.60%), Query Frame = 3 
Query:  225 RALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIRYFRHMESLKHGIVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDF 902
            RA   L K++E YK DQ++Y +FV R+D WF +N K +L +F + D D  G L Y++FKAG++D NIPCNLIELHLL TLLD DGN  ID++EF  GI++F+ +ES   GI+  ES+P       +K++   + ++++   +FL FE    HPGHF+ ++P +T+   ++  I++  +  +    +F +N+K+ +  L E KTL++ G  GG  + P++ ++FYDF
Sbjct:  824 RAQAQLAKMMEGYKADQQRYKEFVNRVDNWFTQNCKVMLELFTKFDRDGNGTLDYDEFKAGMLDMNIPCNLIELHLLCTLLDADGNGDIDYDEFGNGIKHFKEVES--GGIL--ESAP-------LKIVGPREQRFVSVLFRFLIFEQQTNHPGHFEQVVPLETHGHTLVEFIRKRTKLASKDFVLFFDNAKTNQ--LDEYKTLEDHGIHGGPEHAPKQTLVFYDF 1036
[Close]
BLAST of Calcium-dependent protein kinase vs. TrEMBL
Match: V4AIT5 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_159681 PE=4 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 1.339e-35
Identity = 77/246 (31.30%), Postives = 134/246 (54.47%), Query Frame = 3 
Query:  225 RALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIRYFR----HMESLKHGIV---------------EGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDFD 905
            RAL  L K V+  ++D+  YD  + RI++W +E   K + +F ++D    G LTYE+FK+G+ D NIP N  ELHLL  LLD D +  ID+ EF KG+++ R     +E  K  +                 G   PYRE             +++  +++ +TF++ KEHPGH   ++ S   V  ++ I+ ++   L+  + +F   ++++E +LP D +L++ G+ G +  +P+E+ L+YD++
Sbjct:   69 RALAKLEKAVDKIREDEVHYDHLITRINQWLIEISPKAIPLFSKIDVTGDGILTYEEFKSGMFDLNIPLNKTELHLLTKLLDKDDSGEIDYTEFTKGLQHVRKESIELEKDKDEVSLILSERKPIPCPCCKMGVWQPYREKHP----------RYVLVDMRLVTFDSFKEHPGHISVLVHSHLSVYGLIEILVQQTHVLSTKLAVFINKTRNLEAMLPVDSSLEDCGYFGNTKESPQEVTLYYDYN 304
[Close]
BLAST of Calcium-dependent protein kinase vs. TrEMBL
Match: A0A2G8JT08 (Uncharacterized protein OS=Stichopus japonicus OX=307972 GN=BSL78_24272 PE=4 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 3.152e-34
Identity = 79/236 (33.47%), Postives = 129/236 (54.66%), Query Frame = 3 
Query:  225 RALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIRYFRHMESLKHGIV------EGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDFDPAV 914
            +A   L K  E        Y  F++R+++W  EN  K   +F + D+++ G L+Y++FKAG++D + PCN +ELH+ A  LD D N  ID+ EF KG+  F   + L  G V      E ++ P  +       +E    +++    K  TFEN + +PGHF  ++ S  YV  ++++IKEE    T  ++++   ++    LL  DKT++E GFRG +   P+E++L+YDF   V
Sbjct:   22 KARLKLEKSCEELTTQLDLYQSFLDRMNRWMKENGMKAEELFRKFDTNQDGLLSYDEFKAGMLDLDAPCNSVELHVFALQLDKDNNGRIDYLEFAKGLT-FPDSDQLADGPVLRITKEEIQACPCCQLGLWKPYVEPAP-RYVKILFKMATFENLRSYPGHFIKVVHSHIYVYGLISLIKEEMDIATTKMRLYLNKTREASDLLIPDKTMEEYGFRGSTRDCPQEVLLYYDFTTEV 255
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Cavefish
Match: DST (dystonin [Source:HGNC Symbol;Acc:HGNC:1090])

HSP 1 Score: 51.9878 bits (123), Expect = 6.145e-7
Identity = 27/95 (28.42%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  222 NRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            N AL+ L +L E    D   +D + +R  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 1400 NDALDRLEELKEFANFD---FDVWRKRYMRWMNHKKSRVMDFFRRIDKDQDGKVTRQEFIEGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEF 1491
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Cavefish
Match: MACF1 (microtubule actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664])

HSP 1 Score: 48.9062 bits (115), Expect = 7.521e-6
Identity = 25/93 (26.88%), Postives = 51/93 (54.84%), Query Frame = 3 
Query:  228 ALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 5298 ALDRLEELKEFANFD---FDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTSKLEMMAVADIFDRDGDGYIDYYEF 5387
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Cavefish
Match: MACF1 (microtubule actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664])

HSP 1 Score: 48.9062 bits (115), Expect = 7.573e-6
Identity = 25/93 (26.88%), Postives = 51/93 (54.84%), Query Frame = 3 
Query:  228 ALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            AL+ L +L E    D   +D + ++  +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct: 4950 ALDRLEELKEFANFD---FDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTSKLEMMAVADIFDRDGDGYIDYYEF 5039
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Sea Lamprey
Match: MACF1 (microtubule actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664])

HSP 1 Score: 53.5286 bits (127), Expect = 5.488e-8
Identity = 28/93 (30.11%), Postives = 51/93 (54.84%), Query Frame = 3 
Query:  228 ALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            ALE L +L E    D   +D + +R  +W      ++++ F  +D D+ G++T ++F  G++    P N +E+  +A + D DG+  ID+ EF
Sbjct: 3030 ALERLQELKEFENFD---FDVWRKRYMQWMNHKKSRVMDFFRRMDKDQDGKITRQEFIDGILSSRFPTNRLEMTAVADIFDRDGDGYIDYYEF 3119
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Nematostella
Match: EDO37731 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEX3])

HSP 1 Score: 82.0333 bits (201), Expect = 3.081e-17
Identity = 60/243 (24.69%), Postives = 110/243 (45.27%), Query Frame = 3 
Query:  234 ENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIRYFRHMESLKHGIVEGESSPYRETERIIKLLEANDC-------------KWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEH-----LLPEDKTLDELGFRGGSYYN-PRELILFYDFD 905
            + L  L E      K++  F E++ +WF  N  +++++F+  D+D +G+L+Y++F  G+ D   P  + E ++LA  LD D +  ID+NE  K  R  R+    +    + E        R ++L    +C             ++I  +L  L         G F   + SD  +  +  I+K E       I+I R+ +   E      +L +   L + G+ GG+  + P+ + LFYD++
Sbjct:  290 QELADLAEELCNSAKRFSAFPEKMLEWFTFNYYRLIDVFKRFDTDNSGKLSYDEFFLGMQDLGAPVTMTEAYMLAMSLDEDNDETIDYNEVSKIKRTLRNGAVARRVSTDKEKGQEGLDSR-VQLRPCPNCEIGIWEPVLNEAPRYICLHLTNLGLPYKDAFIGQFTTHVKSDITIYGITRIVKSELGGSAVDIRILRKKATKKEQRAETEILDQGLKLRDCGYSGGTSRDKPQHVELFYDYE 531
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Nematostella
Match: EDO41608 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3W3])

HSP 1 Score: 71.2478 bits (173), Expect = 6.177e-16
Identity = 32/72 (44.44%), Postives = 49/72 (68.06%), Query Frame = 3 
Query:  303 IDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGI 518
            ++KW ++N  + +++F   D D  GEL+YE+F AG+ D N P N +EL++LA  LD DGN  +D+ EF KG+
Sbjct:    1 MNKWMIDNYSRAIDLFRRFDVDGDGELSYEEFYAGMRDLNAPANSLELYVLAKKLDRDGNGLLDYLEFSKGL 72
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Nematostella
Match: EDO37706 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SF35])

HSP 1 Score: 51.2174 bits (121), Expect = 4.788e-7
Identity = 29/105 (27.62%), Postives = 59/105 (56.19%), Query Frame = 3 
Query:  228 ALENLNKLVE-----AYKKDQKKYD--QFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIR 521
            ++E L +L E     A K+   K++  ++  R +KW  ++  ++ +IF  +D D+ G+LT EQF  G+++ + P    E+ ++A+  + +G   ID+ EF   ++
Sbjct: 1168 SMERLRRLQEKLERIAIKRASAKFNFPEWKSRFNKWLHDSKSRVQDIFRRMDHDRDGKLTREQFITGVLNTSFPTERWEMEIVASKFERNGL--IDYKEFMNALK 1270
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Nematostella
Match: EDO31006 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZ62])

HSP 1 Score: 46.9802 bits (110), Expect = 6.752e-6
Identity = 31/97 (31.96%), Postives = 57/97 (58.76%), Query Frame = 3 
Query:  225 RALENLNKLVEAYKKDQKKYDQFVERID--KWFVENLK-KILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            R+ + +N+L   +  D KK  + +E I+  K +VE +  +++++F + D D +  +T ++F  GL    +P +  +L +L  LLD DG+  IDF+EF
Sbjct:  442 RSGDVMNQLNAMFSGDTKK-KKILELINMLKEYVERMNYRLVDLFNQFDKDNSLSVTRDEFYTGLKGIGVPMSDKDLTMLIDLLDEDGDGEIDFSEF 537
[Close]
BLAST of Calcium-dependent protein kinase vs. Ensembl Medaka
Match: ENSORLT00000034832.1 (pep primary_assembly:ASM223467v1:11:16837571:16849096:-1 gene:ENSORLG00000026542.1 transcript:ENSORLT00000034832.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.1358 bits (113), Expect = 8.037e-6
Identity = 18/66 (27.27%), Postives = 38/66 (57.58%), Query Frame = 3 
Query:  309 KWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEF 506
            +W      ++++ F  +D D+ G++T ++F  G++    P + +E+  +A + D DG+  ID+ EF
Sbjct:    2 RWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTSKLEMTAVADIFDRDGDGYIDYYEF 67
[Close]
BLAST of Calcium-dependent protein kinase vs. Planmine SMEST
Match: SMESG000033648.1 (SMESG000033648.1)

HSP 1 Score: 515.383 bits (1326), Expect = 0.000e+0
Identity = 274/275 (99.64%), Postives = 274/275 (99.64%), Query Frame = 3 
Query:  123 ISEPVESEVPITNEPXXXXXXXXXXXXXXXXXXNRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIRYFRHMESLKHGIVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDFDPAVWYKHPLILETM 947
            ISEPVESEVPITNEPTKEKGKKKTKKSKKKTPKNRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGI YFRHMESLKHGIVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDFDPAVWYKHPLILETM
Sbjct:   17 ISEPVESEVPITNEPTKEKGKKKTKKSKKKTPKNRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIHYFRHMESLKHGIVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDFDPAVWYKHPLILETM 291
[Close]
BLAST of Calcium-dependent protein kinase vs. Planmine SMEST
Match: SMESG000033648.1 (SMESG000033648.1)

HSP 1 Score: 514.227 bits (1323), Expect = 0.000e+0
Identity = 273/274 (99.64%), Postives = 273/274 (99.64%), Query Frame = 3 
Query:  126 SEPVESEVPITNEPXXXXXXXXXXXXXXXXXXNRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIRYFRHMESLKHGIVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDFDPAVWYKHPLILETM 947
            SEPVESEVPITNEPTKEKGKKKTKKSKKKTPKNRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGI YFRHMESLKHGIVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDFDPAVWYKHPLILETM
Sbjct:    3 SEPVESEVPITNEPTKEKGKKKTKKSKKKTPKNRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIHYFRHMESLKHGIVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDFDPAVWYKHPLILETM 276
[Close]
BLAST of Calcium-dependent protein kinase vs. Planmine SMEST
Match: SMESG000033648.1 (SMESG000033648.1)

HSP 1 Score: 489.574 bits (1259), Expect = 3.729e-176
Identity = 265/274 (96.72%), Postives = 265/274 (96.72%), Query Frame = 3 
Query:  126 SEPVESEVPITNEPXXXXXXXXXXXXXXXXXXNRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIRYFRHMESLKHGIVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDFDPAVWYKHPLILETM 947
            SEPVESEVPITNEPTKEKGKKKTKKSKKKTPKNRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGI YFR        IVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDFDPAVWYKHPLILETM
Sbjct:    3 SEPVESEVPITNEPTKEKGKKKTKKSKKKTPKNRALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIHYFR--------IVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDFDPAVWYKHPLILETM 268
[Close]
BLAST of Calcium-dependent protein kinase vs. Planmine SMEST
Match: SMESG000013944.1 (SMESG000013944.1)

HSP 1 Score: 88.5817 bits (218), Expect = 5.305e-20
Identity = 61/240 (25.42%), Postives = 112/240 (46.67%), Query Frame = 3 
Query:  231 LENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKTGELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFN--EFEKGIRY----FRHMESLKHGIVEGESSPYRETERI-----IKLLEANDCK-----WINCNLKFLTFENHKEHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPEDKTLDELGFRGGSYYNPRELILFYDF 902
            +  L+K ++ Y  D   Y +F +RI+ W+  +   +  +F  +       + YE F AGLID N PCN +E H+LA +LD      I +   EF    +Y     +  E  K  +V+      +E + +       L+EA+ C      W++  +K + F+   EHPG+ +   P +     +   +K+   + T S Q++ +   + ++ +    +  + G  GG    P+E+I++YDF
Sbjct:   24 IARLDKEMKEYVSDSHLYSEFTQRIENWYASS--HVKQMFSLMYCSIKENVNYENFIAGLIDLNAPCNALERHILARILDPQNTGHIAYTDLEFLLWSKYADNDLKKAEMQKKNLVKRADELNKEKKFLDLKDRTSLIEAHTCGMTTIGWLHIYIKMIKFDRFPEHPGNLEMDFPDNIVFEVIAEHVKKALNFPTISFQLYADPYGTTDNKIDLSNSFKDYGIVGGPKLAPQEIIIYYDF 261
The following BLAST results are available for this feature:
BLAST of Calcium-dependent protein kinase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
DST1.315e-627.37dystonin [Source:HGNC Symbol;Acc:HGNC:1090][more]
DST2.422e-627.37dystonin [Source:HGNC Symbol;Acc:HGNC:1090][more]
MACF12.812e-627.37microtubule actin crosslinking factor 1 [Source:HG... [more]
DST4.757e-627.37dystonin [Source:HGNC Symbol;Acc:HGNC:1090][more]
DST7.895e-627.37dystonin [Source:HGNC Symbol;Acc:HGNC:1090][more]
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BLAST of Calcium-dependent protein kinase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium-dependent protein kinase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium-dependent protein kinase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
DST1.168e-628.42dystonin [Source:HGNC Symbol;Acc:HGNC:1090][more]
macf1b2.106e-622.52microtubule actin crosslinking factor 1b [Source:Z... [more]
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BLAST of Calcium-dependent protein kinase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rab11fip24.071e-627.37RAB11 family interacting protein 2 (class I) [Sour... [more]
rab11fip24.139e-627.37RAB11 family interacting protein 2 (class I) [Sour... [more]
rab11fip24.179e-627.37RAB11 family interacting protein 2 (class I) [Sour... [more]
rab11fip24.292e-627.37RAB11 family interacting protein 2 (class I) [Sour... [more]
rab11fip24.989e-627.37RAB11 family interacting protein 2 (class I) [Sour... [more]
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BLAST of Calcium-dependent protein kinase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Dst6.705e-727.37dystonin [Source:MGI Symbol;Acc:MGI:104627][more]
Macf11.957e-627.37microtubule-actin crosslinking factor 1 [Source:MG... [more]
Dst2.220e-627.37dystonin [Source:MGI Symbol;Acc:MGI:104627][more]
Dst3.352e-627.37dystonin [Source:MGI Symbol;Acc:MGI:104627][more]
Dst3.407e-627.37dystonin [Source:MGI Symbol;Acc:MGI:104627][more]
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BLAST of Calcium-dependent protein kinase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|P28582|CDPK_DAUCA3.368e-633.77Calcium-dependent protein kinase OS=Daucus carota ... [more]
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BLAST of Calcium-dependent protein kinase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8I5H98.033e-5340.71Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FHL41.918e-5239.82Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8I3812.428e-5140.71Uncharacterized protein OS=Macrostomum lignano OX=... [more]
V4AIT51.339e-3531.30Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A2G8JT083.152e-3433.47Uncharacterized protein OS=Stichopus japonicus OX=... [more]
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BLAST of Calcium-dependent protein kinase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
DST6.145e-728.42dystonin [Source:HGNC Symbol;Acc:HGNC:1090][more]
MACF17.521e-626.88microtubule actin crosslinking factor 1 [Source:HG... [more]
MACF17.573e-626.88microtubule actin crosslinking factor 1 [Source:HG... [more]
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BLAST of Calcium-dependent protein kinase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MACF15.488e-830.11microtubule actin crosslinking factor 1 [Source:HG... [more]
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BLAST of Calcium-dependent protein kinase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium-dependent protein kinase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 4
Match NameE-valueIdentityDescription
EDO377313.081e-1724.69Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO416086.177e-1644.44Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO377064.788e-727.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO310066.752e-631.96Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Calcium-dependent protein kinase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000034832.18.037e-627.27pep primary_assembly:ASM223467v1:11:16837571:16849... [more]
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BLAST of Calcium-dependent protein kinase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000033648.10.000e+099.64SMESG000033648.1[more]
SMESG000033648.10.000e+099.64SMESG000033648.1[more]
SMESG000033648.13.729e-17696.72SMESG000033648.1[more]
SMESG000013944.15.305e-2025.42SMESG000013944.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034173 ID=SMED30034173|Name=Calcium-dependent protein kinase|organism=Schmidtea mediterranea sexual|type=transcript|length=1034bp
CATTTTCCAAAACTGCAAAAATTAATTCAATTTGTTATATTGTATCAAAT
GAATTTCTATAGAAACGAACTATAATGGTTAAAAAGTTAAAACATCACAA
AAAAGCCAAAAAGAAACAAAAAATAAGTGAACCTGTTGAATCTGAAGTGC
CAATTACAAATGAGCCTACTAAAGAAAAAGGTAAAAAGAAAACCAAAAAA
AGTAAAAAGAAAACTCCCAAAAACAGAGCATTAGAGAATCTTAATAAGTT
AGTTGAAGCATATAAAAAGGATCAGAAAAAATACGATCAATTTGTTGAAA
GAATCGACAAATGGTTCGTAGAAAACTTAAAAAAGATTCTGAATATTTTT
GAAGAGCTCGACAGCGATAAAACTGGTGAACTGACTTATGAGCAATTCAA
GGCAGGATTGATTGACTACAATATTCCCTGCAATTTGATTGAACTCCATT
TGTTAGCGACTCTTCTCGACACAGATGGGAATTTGGGAATAGATTTCAAT
GAGTTTGAGAAAGGAATTCGTTATTTTCGTCATATGGAAAGTTTAAAACA
TGGAATAGTTGAAGGTGAAAGCTCACCATACAGGGAAACAGAGAGAATTA
TAAAATTATTGGAAGCTAATGATTGCAAGTGGATAAATTGTAATCTCAAA
TTTCTCACTTTCGAAAACCATAAAGAACACCCTGGCCATTTCCAATATAT
TTTACCATCGGATACTTATGTGTTACACATGATGAATATTATAAAAGAAG
AAAAAAGATATTTAACAGATAGCATCCAAATTTTTAGAGAGAATTCGAAA
TCAATTGAGCACTTGTTGCCAGAAGACAAAACTTTGGATGAATTGGGATT
TCGTGGCGGATCATATTACAACCCTAGGGAACTAATACTTTTTTACGATT
TTGATCCAGCTGTTTGGTACAAACACCCTTTGATATTGGAAACGATGTGA
TTTTTTCAATTCAACTACATTTTTTAATAAATTGATTTTTTTTGTATAAC
ATAATATTATAAAATTTTCTTTTACCCATTTACA
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protein sequence of SMED30034173-orf-1

>SMED30034173-orf-1 ID=SMED30034173-orf-1|Name=SMED30034173-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=292bp
MVKKLKHHKKAKKKQKISEPVESEVPITNEPTKEKGKKKTKKSKKKTPKN
RALENLNKLVEAYKKDQKKYDQFVERIDKWFVENLKKILNIFEELDSDKT
GELTYEQFKAGLIDYNIPCNLIELHLLATLLDTDGNLGIDFNEFEKGIRY
FRHMESLKHGIVEGESSPYRETERIIKLLEANDCKWINCNLKFLTFENHK
EHPGHFQYILPSDTYVLHMMNIIKEEKRYLTDSIQIFRENSKSIEHLLPE
DKTLDELGFRGGSYYNPRELILFYDFDPAVWYKHPLILETM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004672protein kinase activity
GO:0005524ATP binding
GO:0016740transferase activity
GO:0005509calcium ion binding
GO:0016301kinase activity
Vocabulary: biological process
TermDefinition
GO:0006468protein phosphorylation
GO:0016310phosphorylation
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 49..69
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 32..188
e-value: 4.1E-13
score: 51.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..50
IPR002048EF-hand domainSMARTSM00054efh_1coord: 87..115
e-value: 0.57
score: 19.2
coord: 123..151
e-value: 16.0
score: 8.1
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 83..118
score: 11.78
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 130..154
score: 7.205
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 81..153