Peptidase S1 domain-containing protein

Overview
NamePeptidase S1 domain-containing protein
Smed IDSMED30034156
Length (bp)1055
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Peptidase S1 domain-containing protein (SMED30034156) t-SNE clustered cells

Violin plots show distribution of expression levels for Peptidase S1 domain-containing protein (SMED30034156) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30034156

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30034156h1SMcG0002606 SmedASXL_013746SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30034156h1SMcG0002606 dd_Smed_v4_7481_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30034156h1SMcG0002606 dd_Smed_v4_7481_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30034156h1SMcG0002606 dd_Smed_v4_7481_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Human
Match: TPSAB1 (tryptase alpha/beta 1 [Source:HGNC Symbol;Acc:HGNC:12019])

HSP 1 Score: 112.849 bits (281), Expect = 2.338e-28
Identity = 85/298 (28.52%), Postives = 130/298 (43.62%), Query Frame = 1
Query:   97 IVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLK--IQSNKFCEKVYNQGADLNDEHE------FCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQ 966
            IV G++AP  K+ W +SL+   P    ++ CG SLI  QW+LTAAHC              P +    A   +L  +++ Y+ +                                 L VS+II+HP +   +I  DIAL++L+ P+    ++  +  + LP  +++ P    G  C + GWG   N   +     +  +K  I  N  C+  Y+ GA   D+         CAG  +     C+GDSG  L CK    W  AGV S        N PGI+TRV+Y++ WI   + K+
Sbjct:   31 IVGGQEAPRSKWPWQVSLRVHGPY--WMHFCGGSLIHPQWVLTAAHC------------VGPDVKDLAALRVQLREQHLYYQDQL--------------------------------LPVSRIIVHPQFYTAQIGADIALLELEEPVN---VSSHVHTVTLPPASETFP---PGMPCWVTGWGDVDNDERLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIVRDDMLCAGNTRRDS--CQGDSGGPLVCKVNGTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPKK 274          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Human
Match: KLKB1 (kallikrein B1 [Source:HGNC Symbol;Acc:HGNC:6371])

HSP 1 Score: 117.472 bits (293), Expect = 2.370e-28
Identity = 91/290 (31.38%), Postives = 130/290 (44.83%), Query Frame = 1
Query:   88 SKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCF--LAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILE 951
            S RIV G ++   ++ W +SL+  +     +  CG SLI  QW+LTAAHCF  L  Q+          +W   +G   L                      SD          +  P S     + +II+H NYK  E   DIAL+KLQ+P+      EF + I LPS+ D+     I   C + GWG     G I    Q  N+ + +N+ C+K Y Q   +  +   CAG  + G   C+GDSG  L CK    W L G+ S          PG++T+V+ ++ WILE
Sbjct:  388 STRIVGGTNSSWGEWPWQVSLQVKLTAQRHL--CGGSLIGHQWVLTAAHCFDGLPLQD----------VWRIYSGILNL----------------------SDIT--------KDTPFS----QIKEIIIHQNYKVSEGNHDIALIKLQAPLN---YTEFQKPICLPSKGDTS---TIYTNCWVTGWGFSKEKGEIQNILQKVNIPLVTNEECQKRY-QDYKIT-QRMVCAGYKEGGKDACKGDSGGPLVCKHNGMWRLVGITSWGEGCARREQPGVYTKVAEYMDWILE 623          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Human
Match: TPSAB1 (tryptase alpha/beta 1 [Source:HGNC Symbol;Acc:HGNC:12019])

HSP 1 Score: 112.849 bits (281), Expect = 2.733e-28
Identity = 85/298 (28.52%), Postives = 130/298 (43.62%), Query Frame = 1
Query:   97 IVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLK--IQSNKFCEKVYNQGADLNDEHE------FCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQ 966
            IV G++AP  K+ W +SL+   P    ++ CG SLI  QW+LTAAHC              P +    A   +L  +++ Y+ +                                 L VS+II+HP +   +I  DIAL++L+ P+    ++  +  + LP  +++ P    G  C + GWG   N   +     +  +K  I  N  C+  Y+ GA   D+         CAG  +     C+GDSG  L CK    W  AGV S        N PGI+TRV+Y++ WI   + K+
Sbjct:   38 IVGGQEAPRSKWPWQVSLRVHGPY--WMHFCGGSLIHPQWVLTAAHC------------VGPDVKDLAALRVQLREQHLYYQDQL--------------------------------LPVSRIIVHPQFYTAQIGADIALLELEEPVN---VSSHVHTVTLPPASETFP---PGMPCWVTGWGDVDNDERLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIVRDDMLCAGNTRRDS--CQGDSGGPLVCKVNGTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPKK 281          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Human
Match: TPSB2 (tryptase beta 2 (gene/pseudogene) [Source:HGNC Symbol;Acc:HGNC:14120])

HSP 1 Score: 112.464 bits (280), Expect = 3.118e-28
Identity = 84/298 (28.19%), Postives = 130/298 (43.62%), Query Frame = 1
Query:   97 IVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLK--IQSNKFCEKVYNQGADLNDEHE------FCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQ 966
            IV G++AP  K+ W +SL+  +     ++ CG SLI  QW+LTAAHC              P +    A   +L  +++ Y+ +                                 L VS+II+HP +   +I  DIAL++L+ P+    ++  +  + LP  +++ P    G  C + GWG   N   +     +  +K  I  N  C+  Y+ GA   D+         CAG  +     C+GDSG  L CK    W  AGV S        N PGI+TRV+Y++ WI   + K+
Sbjct:   31 IVGGQEAPRSKWPWQVSLR--VRDRYWMHFCGGSLIHPQWVLTAAHC------------VGPDVKDLAALRVQLREQHLYYQDQL--------------------------------LPVSRIIVHPQFYTAQIGADIALLELEEPVN---VSSHVHTVTLPPASETFP---PGMPCWVTGWGDVDNDERLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIVRDDMLCAGNTRRDS--CQGDSGGPLVCKVNGTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPKK 274          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Human
Match: TPSB2 (tryptase beta 2 (gene/pseudogene) [Source:HGNC Symbol;Acc:HGNC:14120])

HSP 1 Score: 112.464 bits (280), Expect = 3.605e-28
Identity = 84/298 (28.19%), Postives = 130/298 (43.62%), Query Frame = 1
Query:   97 IVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLK--IQSNKFCEKVYNQGADLNDEHE------FCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQ 966
            IV G++AP  K+ W +SL+  +     ++ CG SLI  QW+LTAAHC              P +    A   +L  +++ Y+ +                                 L VS+II+HP +   +I  DIAL++L+ P+    ++  +  + LP  +++ P    G  C + GWG   N   +     +  +K  I  N  C+  Y+ GA   D+         CAG  +     C+GDSG  L CK    W  AGV S        N PGI+TRV+Y++ WI   + K+
Sbjct:   38 IVGGQEAPRSKWPWQVSLR--VRDRYWMHFCGGSLIHPQWVLTAAHC------------VGPDVKDLAALRVQLREQHLYYQDQL--------------------------------LPVSRIIVHPQFYTAQIGADIALLELEEPVN---VSSHVHTVTLPPASETFP---PGMPCWVTGWGDVDNDERLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIVRDDMLCAGNTRRDS--CQGDSGGPLVCKVNGTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPKK 281          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Celegans
Match: svh-1 (HGF/MSP/plasminogen-like protein [Source:UniProtKB/TrEMBL;Acc:Q17800])

HSP 1 Score: 92.4337 bits (228), Expect = 3.062e-20
Identity = 84/292 (28.77%), Postives = 128/292 (43.84%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGN-KWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQ 966
            R+V G +     + W+ +L+         + CGAS++DK  L+TAAHCF   +E                                        E  S ++   G WD  +   +    ++ +I  +P YK D    DIA++++  P  E   NE+ + I LPS+ D    P  G+ C++ GWG  + G   +ER Q A + I  N+F     +Q         FCAG  + G   C+GDSG   AC++ +  + LAGV S          PGI+T V+ ++SWI   IN Q
Sbjct:  712 RVVGGFETVPGAFPWTAALRNKA---TKAHHCGASILDKTHLITAAHCF---EED---------------------------------------ERVSSYEVVVGDWDNNQTDGNEQIFYLQRIHFYPLYK-DIFSHDIAILEIPYPGIE--FNEYAQPICLPSK-DFVYTP--GRQCVVSGWG--SMGLRYAERLQAALIPII-NRFDCVNSSQIYSSMSRSAFCAGYLEGGIDSCQGDSGGPFACRREDGAFVLAGVISWGDGCAQKKQPGIYTMVAPYLSWISAIINGQ 949          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Celegans
Match: try-1 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q23528])

HSP 1 Score: 78.9518 bits (193), Expect = 1.846e-16
Identity = 67/299 (22.41%), Postives = 116/299 (38.80%), Query Frame = 1
Query:   76 FNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPD-EIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERA-QIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVAS-ATHAELPGNYPGIFTRVSYFISWILENINK 963
            +  +  R++ G ++    + W++ L   +      + CG SLID  ++LTAAHCF  A+++       P  +  R G  +                              G     R         V+ + +HP Y       +D A+M++  P+        + +  LP+  +        ++C++ GWG    G S+S    +  ++ + S  FC  + N    ++     CAG        C+GDSG  L C +   W L GV S       PG  PG++  V    +WI   +N+
Sbjct:   51 YVTLDHRLIGGSESSPHSWPWTVQLLSRL----GHHRCGGSLIDPNFVLTAAHCF--AKDR------RPTSYSVRVGGHR-----------------------------SGSGSPHR---------VTAVSIHPWYNIGFPSSYDFAIMRIHPPVNTSTTARPICLPSLPAVEN--------RLCVVTGWGSTIEGSSLSAPTLREIHVPLLSTLFCSSLPNYIGRIHLPSMLCAGYSYGKIDSCQGDSGGPLMCARDGHWELTGVVSWGIGCARPG-MPGVYGNVHSASTWINLEMNR 290          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Celegans
Match: try-3 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q94176])

HSP 1 Score: 71.633 bits (174), Expect = 7.222e-14
Identity = 80/335 (23.88%), Postives = 133/335 (39.70%), Query Frame = 1
Query:   31 FILLCNLFVLIYSRSFN--------VISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKF-------CEKVYNQGADLNDE---HEFCAGKYKSGHGLCEGDSGSGLACKKGN-KWTLAGVASATHAEL-----PGNYPGIFTRVSYFISWILENINK 963
            F++LC +F+  +S + N        + S RI+ G ++ +D   W   L       + +  CGA++ID  WL+TAAHC L  Q +S +   +P                                          K ++ R+        V +  +H  Y     + DIAL+++ S + +      L + P+    D     K  +  ++IG+G      S  E   I +  +QS          C K +   + L+ +   ++ CAG Y   HG   GDSG  L   K N ++   G+ S     L      G +PG++TR+S ++ WI   I K
Sbjct:    8 FLVLCFVFITKHSNAINLCEESYKPIFSFRIIGG-NSIDDGANWMAKLVSYGDNGQGIL-CGATVIDDFWLVTAAHCALQLQTRSFVYVREP------------------------------------------KNNRERS------FSVKEAYIHSGYNNQTADNDIALLRISSDLSK------LGIKPVCLVHDDSKLLKQYKNGVVIGYGLTLGEDSSGEPKLINSQTLQSTSVPIISDDDCVKTWRFLSLLSVKITGYQICAGAYL--HGTAPGDSGGPLLIHKSNGEYVQIGITSYGADGLDGVIDQGKFPGVYTRISKYVPWIQGVIGK 284          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Celegans
Match: try-3 (TRYpsin-like protease [Source:UniProtKB/TrEMBL;Acc:Q94176])

HSP 1 Score: 63.5438 bits (153), Expect = 2.422e-11
Identity = 67/275 (24.36%), Postives = 109/275 (39.64%), Query Frame = 1
Query:  187 CGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKF-------CEKVYNQGADLNDE---HEFCAGKYKSGHGLCEGDSGSGLACKKGN-KWTLAGVASATHAEL-----PGNYPGIFTRVSYFISWILENINK 963
            CGA++ID  WL+TAAHC L  Q +S +   +P                   ++  E+++                       E+Y+         H  Y     + DIAL+++ S + +      L + P+    D     K  +  ++IG+G      S  E   I +  +QS          C K +   + L+ +   ++ CAG Y   HG   GDSG  L   K N ++   G+ S     L      G +PG++TR+S ++ WI   I K
Sbjct:   16 CGATVIDDFWLVTAAHCALQLQTRSFVYVREP-------------------KNNRERSFS--------------------VKEAYI---------HSGYNNQTADNDIALLRISSDLSK------LGIKPVCLVHDDSKLLKQYKNGVVIGYGLTLGEDSSGEPKLINSQTLQSTSVPIISDDDCVKTWRFLSLLSVKITGYQICAGAYL--HGTAPGDSGGPLLIHKSNGEYVQIGITSYGADGLDGVIDQGKFPGVYTRISKYVPWIQGVIGK 234          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Fly
Match: CG18735 (gene:FBgn0042098 transcript:FBtr0071744)

HSP 1 Score: 97.4413 bits (241), Expect = 1.799e-22
Identity = 81/291 (27.84%), Postives = 122/291 (41.92%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLK--GDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKY-KSGHGLCEGDSGSGL-ACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILEN 954
            RIV G++    +Y W I L   G+       + CGASL++ Q+ LTAAHC                 +H     R LE       H  + ++                          +D  VS++++HP Y     + DIAL++   P+    ++     +P PSE  +      GQ  ++ GWG  + GG IS+  Q   + I S + C    N G     ++  CAG   + G   C+GDSG  +     G+ + LAG+ S        N PG++TRV  F  WI EN
Sbjct:   82 RIVGGQETEVHEYPWMIMLMWFGN-------FYCGASLVNDQYALTAAHCVNG-------------FYHRLITVRLLE-------HNRQDSHV-----------------------KIVDRRVSRVLIHPKYSTRNFDSDIALIRFNEPVRLG-IDMHPVCMPTPSENYA------GQTAVVTGWGALSEGGPISDTLQEVEVPILSQEECRN-SNYGESKITDNMICAGYVEQGGKDSCQGDSGGPMHVLGSGDAYQLAGIVSWGEGCAKPNAPGVYTRVGSFNDWIAEN 314          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Fly
Match: CG3355 (gene:FBgn0031619 transcript:FBtr0077376)

HSP 1 Score: 96.2857 bits (238), Expect = 2.471e-22
Identity = 82/289 (28.37%), Postives = 126/289 (43.60%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISL-KGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKS-GHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILEN 954
            RIV G+    +KY W+  L KG          CG SLI+ +++LTAAHC    +++  +              R L+ +                               R + +  +   V +  +HPNY P+ I  D+AL+KL+SP+    L   +  + LP EA+       G+  ++ GWG    GG  S   Q  N+ + +N  C +   +  D   E   CAG  +  G   C+GDSG  L   +G ++ LAGV S  +     N PG++ RVS F+ WI +N
Sbjct:   75 RIVGGQQVRSNKYPWTAQLVKG---RHYPRLFCGGSLINDRYVLTAAHCVHGNRDQITI--------------RLLQID-------------------------------RSSRDPGIVRKVVQTTVHPNYDPNRIVNDVALLKLESPVP---LTGNMRPVCLP-EANHN---FDGKTAVVAGWGLIKEGGVTSNYLQEVNVPVITNAQCRQTRYK--DKIAEVMLCAGLVQQGGKDACQGDSGGPLIVNEG-RYKLAGVVSFGYGCAQKNAPGVYARVSKFLDWIRKN 305          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Fly
Match: Sb (gene:FBgn0003319 transcript:FBtr0344008)

HSP 1 Score: 97.4413 bits (241), Expect = 8.942e-22
Identity = 85/293 (29.01%), Postives = 130/293 (44.37%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLK-GDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGA--DLNDEHEFCAGKYKSGHGLCEGDSGSGLACK-KGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENIN 960
            RIV GK A   ++ W +S++        + + CG +LI++ W+ TA HC        LL S   I    R G      EY  + H  E+                           Y++  V+K ++HP Y     E+D+AL+KL+ P+E       +  I LP E DS     IG    + GWG  + GG++    Q  ++ I SN  C+ ++ +    +   +   CAG    G   C+GDSG  L  K +  ++ LAG+ S        N PG+ TR+S F  WILE++ 
Sbjct:  543 RIVGGKSAAFGRWPWQVSVRRTSFFGFSSTHRCGGALINENWIATAGHCV-----DDLLISQIRI----RVG------EY-DFSHVQEQL-------------------------PYIERGVAKKVVHPKYSFLTYEYDLALVKLEQPLE---FAPHVSPICLP-ETDSL---LIGMNATVTGWGRLSEGGTLPSVLQEVSVPIVSNDNCKSMFMRAGRQEFIPDIFLCAGYETGGQDSCQGDSGGPLQAKSQDGRFFLAGIISWGIGCAEANLPGVCTRISKFTPWILEHVR 787          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Fly
Match: Sb (gene:FBgn0003319 transcript:FBtr0083250)

HSP 1 Score: 97.4413 bits (241), Expect = 8.942e-22
Identity = 85/293 (29.01%), Postives = 130/293 (44.37%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLK-GDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGA--DLNDEHEFCAGKYKSGHGLCEGDSGSGLACK-KGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENIN 960
            RIV GK A   ++ W +S++        + + CG +LI++ W+ TA HC        LL S   I    R G      EY  + H  E+                           Y++  V+K ++HP Y     E+D+AL+KL+ P+E       +  I LP E DS     IG    + GWG  + GG++    Q  ++ I SN  C+ ++ +    +   +   CAG    G   C+GDSG  L  K +  ++ LAG+ S        N PG+ TR+S F  WILE++ 
Sbjct:  543 RIVGGKSAAFGRWPWQVSVRRTSFFGFSSTHRCGGALINENWIATAGHCV-----DDLLISQIRI----RVG------EY-DFSHVQEQL-------------------------PYIERGVAKKVVHPKYSFLTYEYDLALVKLEQPLE---FAPHVSPICLP-ETDSL---LIGMNATVTGWGRLSEGGTLPSVLQEVSVPIVSNDNCKSMFMRAGRQEFIPDIFLCAGYETGGQDSCQGDSGGPLQAKSQDGRFFLAGIISWGIGCAEANLPGVCTRISKFTPWILEHVR 787          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Fly
Match: mas (gene:FBgn0011653 transcript:FBtr0332659)

HSP 1 Score: 96.6709 bits (239), Expect = 1.777e-21
Identity = 81/295 (27.46%), Postives = 127/295 (43.05%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGAD---LNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN 969
            R+V G+D    ++ W ++L   +      Y CGA+LI  QW+LTAAHC       +++ S D I  + R G   L  +Y                      G+ G             L V+   +H N+    ++ DIAL+KL    E   L + + ++ LP+   S      G+ C + G+G     G I  R + A + I S+  C +  N   +   +     FCAG  + GH  C+GD G  L C+    + LAG+ S        + PG++ + S FI WI + I+  N
Sbjct:  801 RVVGGEDGENGEWCWQVALINSL----NQYLCGAALIGTQWVLTAAHCV-----TNIVRSGDAI--YVRVGDYDLTRKY----------------------GSPGAQT----------LRVATTYIHHNHNSQTLDNDIALLKLHGQAE---LRDGVCLVCLPARGVSH---AAGKRCTVTGYGYMGEAGPIPLRVREAEIPIVSDTECIRKVNAVTEKIFILPASSFCAGG-EEGHDACQGDGGGPLVCQDDGFYELAGLVSWGFGCGRQDVPGVYVKTSSFIGWINQIISVNN 1045          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Zebrafish
Match: CR352265.1 (pep chromosome:GRCz11:24:21813127:21819010:-1 gene:ENSDARG00000062758.6 transcript:ENSDART00000091096.6 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR352265.1)

HSP 1 Score: 123.635 bits (309), Expect = 2.513e-32
Identity = 82/294 (27.89%), Postives = 129/294 (43.88%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLE--VIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLAC-KKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQ 966
            +IV G DA    + W +SL+     +   + CGASL+  +WL++AAHCF   Q+   +  +D   W A  G R + +                                     +     + +I+LH  Y     ++DIAL++L +P+   + NE ++   +P PS   +      G  C + GWG  T  G ++   Q A + I ++  C K+Y+   D       CAG  + G   C+GDSG  L C ++G +W LAG+ S        N PG++TRV  F  WI +    Q
Sbjct:   54 KIVGGTDAQAGSWPWQVSLQ----MERYGHVCGASLVASRWLVSAAHCF---QDSDAIKYSDARSWRAYMGMRVMNS----------------------------------VSNAAATRQIRRIVLHSQYDQFTSDYDIALLELSAPV---FFNELVQPVCVPAPSHVFTS-----GTSCFVTGWGVLTEEGELATLLQEATVNIINHNTCNKMYD---DAVTPRMLCAGNIQGGVDACQGDSGGPLVCLERGRRWFLAGIVSWGEGCARQNRPGVYTRVIKFTDWIHQQTKGQ 295          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Zebrafish
Match: tmprss3b (transmembrane serine protease 3b [Source:ZFIN;Acc:ZDB-GENE-030616-522])

HSP 1 Score: 114.39 bits (285), Expect = 1.073e-28
Identity = 96/301 (31.89%), Postives = 132/301 (43.85%), Query Frame = 1
Query:   58 LIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLH-VSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENI 957
            L YSRS   IS RIV G  +   +  W +SL       +  Y CG S+I + W+LTAAHC     +        P+LW   AG   L                                     P S  + H V KII H N++     +DIAL+KL  P+    LN+ +  I LP+  +S    K GQMC++ GWG  T     S    +A + + SNK C K+     ++  E       +  G G C+GDSG  LAC +G+ WTL G  S        N PGI+T +S  ++WI E +
Sbjct:   78 LGYSRS--AISSRIVGGNVSKSGQVPWQVSLH-----YQNQYLCGGSIISESWILTAAHCVFGFAQ--------PVLWDVYAGLINL-------------------------------------PLSKAEAHSVEKIIYHANFRSKSFSYDIALIKLALPLT---LNDQIAPICLPNYGESF---KNGQMCLISGWG-ATVDRETSLSLHVAQVPLLSNKECRKLGLTNWNVCTE-------FLRGVGTCQGDSGGPLAC-QGSAWTLVGTGSWDENCGKVNKPGIYTSISEALTWIQEQM 311          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Zebrafish
Match: tmprss3a (transmembrane serine protease 3a [Source:ZFIN;Acc:ZDB-GENE-070912-70])

HSP 1 Score: 114.005 bits (284), Expect = 1.562e-27
Identity = 88/288 (30.56%), Postives = 123/288 (42.71%), Query Frame = 1
Query:   88 SKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEK--VYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWI 945
            S RIV G  + E ++ W +SL       +  + CG S+I  +W+LTAAHC              P+ W   AG  +L    VK                                       V KII H  Y+P  ++ DIALMKL  P+     N  +E I LP+  +     + G+MC + GWG   +GG  S     A++ + SNK C +  VY QG         CAG    G   C+GDSG  LAC+  + W L G  S        N PG++TR++  ++WI
Sbjct:  276 SARIVGGNLSAEGQFPWQVSLH-----FQNEHLCGGSIITSRWILTAAHCVYGIAY--------PMYWMVYAGLTELPLNAVK------------------------------------AFAVEKIIYHSRYRPKGLDHDIALMKLAQPLT---FNGMVEPICLPNFGEQF---EDGKMCWISGWGATEDGGDASVSQHCASVPLISNKACSQPEVY-QG--YLTAGMICAGYLDGGTDSCQGDSGGPLACEDSSIWKLVGATSWGQGCAEKNKPGVYTRITQSLTWI 505          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Zebrafish
Match: tmprss3a (transmembrane serine protease 3a [Source:ZFIN;Acc:ZDB-GENE-070912-70])

HSP 1 Score: 113.62 bits (283), Expect = 1.626e-27
Identity = 88/288 (30.56%), Postives = 123/288 (42.71%), Query Frame = 1
Query:   88 SKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEK--VYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWI 945
            S RIV G  + E ++ W +SL       +  + CG S+I  +W+LTAAHC              P+ W   AG  +L    VK                                       V KII H  Y+P  ++ DIALMKL  P+     N  +E I LP+  +     + G+MC + GWG   +GG  S     A++ + SNK C +  VY QG         CAG    G   C+GDSG  LAC+  + W L G  S        N PG++TR++  ++WI
Sbjct:  233 SARIVGGNLSAEGQFPWQVSLH-----FQNEHLCGGSIITSRWILTAAHCVYGIAY--------PMYWMVYAGLTELPLNAVKA------------------------------------FAVEKIIYHSRYRPKGLDHDIALMKLAQPLT---FNGMVEPICLPNFGEQF---EDGKMCWISGWGATEDGGDASVSQHCASVPLISNKACSQPEVY-QG--YLTAGMICAGYLDGGTDSCQGDSGGPLACEDSSIWKLVGATSWGQGCAEKNKPGVYTRITQSLTWI 462          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Zebrafish
Match: zgc:100868 (zgc:100868 [Source:ZFIN;Acc:ZDB-GENE-040801-33])

HSP 1 Score: 110.923 bits (276), Expect = 1.511e-26
Identity = 87/294 (29.59%), Postives = 130/294 (44.22%), Query Frame = 1
Query:   85 ISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESY-LDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERA--QIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENI 957
            ++ RIV G++AP   + W +SL+ D       + CG SLI+ QW+LTAAHCF       LL            G +KL +                                    ESY +   VS II HPNY  D  + DI L++L S +     + ++  I L + +DS  +   G +  + GWG    G S+      Q   + I  N+ C  +Y  G     ++  CAG  + G   C+GDSG  +  K+G+ W  +G+ S        N+PG++TRVS + SWI + I
Sbjct:   33 LNSRIVGGQNAPVGAWPWQVSLQRD-----GSHFCGGSLINNQWILTAAHCFPNPSTTGLL---------VYLGLQKLASF-----------------------------------ESYSMSSAVSNIIKHPNYNSDTEDNDITLLQLASTVS---FSNYIRPICL-AASDSTFFN--GTLVWITGWGNTATGVSLPSPGTLQEVQVPIVGNRKCNCLY--GVSKITDNMVCAGLLQGGKDSCQGDSGGPMVSKQGSVWIQSGIVSFGTGCAQPNFPGVYTRVSKYQSWIQQRI 269          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Xenopus
Match: TMPRSS9 (transmembrane serine protease 9 [Source:NCBI gene;Acc:100489279])

HSP 1 Score: 120.168 bits (300), Expect = 3.617e-29
Identity = 85/293 (29.01%), Postives = 135/293 (46.08%), Query Frame = 1
Query:   88 SKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWG-CKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKK-GNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENIN 960
            + RIV G DA + ++ W +SL+     +   + CGA++I  +WL++AAHCF   Q        DP +W A                         Y  T+   GTD          S +   +  II HP+Y PD  ++D+A+++L SP++    N++ + + LP      P   +G+ CI+ GWG  K +     E  Q A + I     C  +Y   +++  E   CAG  +     C+GDSG  L C++   K+ LAG+ S          PG++ RVS   +WIL+ I+
Sbjct:  235 TNRIVGGSDATKGEFPWQVSLR-----ENNEHFCGATVIGDKWLVSAAHCFNDFQ--------DPAVWVA-------------------------YIATTSLSGTD---------SSTVKATIRNIIKHPSYDPDTADYDVAVLELDSPLK---FNKYTQPVCLPDPTHVFP---VGKKCIITGWGYLKEDNLVKPEVLQKATVAIMDQSLCNSLY---SNVVTERMLCAGYLEGKIDSCQGDSGGPLVCEEPSGKFFLAGIVSWGVGCAEARRPGVYVRVSKIRNWILDIIS 471          

HSP 2 Score: 105.531 bits (262), Expect = 3.443e-24
Identity = 88/310 (28.39%), Postives = 137/310 (44.19%), Query Frame = 1
Query:   31 FILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKK-GNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENI 957
            FIL C    ++    FN    +IV G  +   ++ W +SL          + CGA LI  +WLL+AAHCF           +DP LW A                         Y  T   +G +G+ +K           + +I  HP Y    ++ D+AL++L SP+   Y N  +  I LP  +   P    G  C + GWG    GG++S + Q A++ I  ++ C+K Y            CAG  + G   C GD+G  LAC++   +W LAG+ S  +      +PG++TR++   +WI +N+
Sbjct:  847 FILDCGFAPVL---PFN----KIVGGSGSVRGEWPWQVSLW----LRRKEHKCGAVLISDRWLLSAAHCFDIY--------SDPKLWAA-------------------------YLGTPFLNGVEGRVEK-----------IFRIHKHPFYNVYTLDNDVALLELPSPLT--YTN-LIRPICLPDISHIFPE---GTRCFITGWGSTKEGGAMSRQLQKASVSIVGDQTCKKFYPIQI---SPRMLCAGFMQGGVDSCSGDAGGPLACREPSGRWFLAGITSWGYGCARPYFPGVYTRITSVRNWIGQNL 1092          

HSP 3 Score: 91.6633 bits (226), Expect = 1.415e-19
Identity = 78/290 (26.90%), Postives = 125/290 (43.10%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWG-CKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKG-NKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENI 957
            +IV G DA   +  W  SLK     + + + CGA++I  +WL++AAHCF         N T      A  G+  L                          G D            + + + ++I HP++ P  +++D+A+++L S +     N++++ + LPS     P    G  C++ GWG  K    S  E  Q A++ I   K C  +YN       E   CAG        C+GDSG  LAC++    + LAG+ S          PG+++RV+    WIL+ +
Sbjct:  530 KIVGGLDAVRGEIPWQASLK-----EGSRHFCGATIIGDRWLVSAAHCF---------NQTKVDQVTAHMGSTALS-------------------------GAD---------TIAIKISLKRVIQHPHFNPLTLDFDVAVLELASSLT---FNKYVQPVCLPSALQKFP---AGWKCMISGWGNIKEGNVSKPEVLQKASVGIIDQKICSVLYNFSI---TERMICAGFLDGKVDSCQGDSGGPLACEESPGIFFLAGIVSWGIGCAQAKKPGVYSRVTKLKDWILDTV 762          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Xenopus
Match: TMPRSS9 (transmembrane serine protease 9 [Source:NCBI gene;Acc:100489279])

HSP 1 Score: 119.783 bits (299), Expect = 5.266e-29
Identity = 85/293 (29.01%), Postives = 135/293 (46.08%), Query Frame = 1
Query:   88 SKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWG-CKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKK-GNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENIN 960
            + RIV G DA + ++ W +SL+     +   + CGA++I  +WL++AAHCF   Q        DP +W A                         Y  T+   GTD          S +   +  II HP+Y PD  ++D+A+++L SP++    N++ + + LP      P   +G+ CI+ GWG  K +     E  Q A + I     C  +Y   +++  E   CAG  +     C+GDSG  L C++   K+ LAG+ S          PG++ RVS   +WIL+ I+
Sbjct:   48 TNRIVGGSDATKGEFPWQVSLR-----ENNEHFCGATVIGDKWLVSAAHCFNDFQ--------DPAVWVA-------------------------YIATTSLSGTD---------SSTVKATIRNIIKHPSYDPDTADYDVAVLELDSPLK---FNKYTQPVCLPDPTHVFP---VGKKCIITGWGYLKEDNLVKPEVLQKATVAIMDQSLCNSLY---SNVVTERMLCAGYLEGKIDSCQGDSGGPLVCEEPSGKFFLAGIVSWGVGCAEARRPGVYVRVSKIRNWILDIIS 284          

HSP 2 Score: 105.145 bits (261), Expect = 4.195e-24
Identity = 88/310 (28.39%), Postives = 137/310 (44.19%), Query Frame = 1
Query:   31 FILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKK-GNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENI 957
            FIL C    ++    FN    +IV G  +   ++ W +SL          + CGA LI  +WLL+AAHCF           +DP LW A                         Y  T   +G +G+ +K           + +I  HP Y    ++ D+AL++L SP+   Y N  +  I LP  +   P    G  C + GWG    GG++S + Q A++ I  ++ C+K Y            CAG  + G   C GD+G  LAC++   +W LAG+ S  +      +PG++TR++   +WI +N+
Sbjct:  645 FILDCGFAPVL---PFN----KIVGGSGSVRGEWPWQVSLW----LRRKEHKCGAVLISDRWLLSAAHCFDIY--------SDPKLWAA-------------------------YLGTPFLNGVEGRVEK-----------IFRIHKHPFYNVYTLDNDVALLELPSPLT--YTN-LIRPICLPDISHIFPE---GTRCFITGWGSTKEGGAMSRQLQKASVSIVGDQTCKKFYPIQI---SPRMLCAGFMQGGVDSCSGDAGGPLACREPSGRWFLAGITSWGYGCARPYFPGVYTRITSVRNWIGQNL 890          

HSP 3 Score: 91.2781 bits (225), Expect = 1.824e-19
Identity = 78/290 (26.90%), Postives = 125/290 (43.10%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWG-CKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKG-NKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENI 957
            +IV G DA   +  W  SLK     + + + CGA++I  +WL++AAHCF         N T      A  G+  L                          G D            + + + ++I HP++ P  +++D+A+++L S +     N++++ + LPS     P    G  C++ GWG  K    S  E  Q A++ I   K C  +YN       E   CAG        C+GDSG  LAC++    + LAG+ S          PG+++RV+    WIL+ +
Sbjct:  343 KIVGGLDAVRGEIPWQASLK-----EGSRHFCGATIIGDRWLVSAAHCF---------NQTKVDQVTAHMGSTALS-------------------------GAD---------TIAIKISLKRVIQHPHFNPLTLDFDVAVLELASSLT---FNKYVQPVCLPSALQKFP---AGWKCMISGWGNIKEGNVSKPEVLQKASVGIIDQKICSVLYNFSI---TERMICAGFLDGKVDSCQGDSGGPLACEESPGIFFLAGIVSWGIGCAQAKKPGVYSRVTKLKDWILDTV 575          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Xenopus
Match: pcdh8l (protocadherin-8-like [Source:Xenbase;Acc:XB-GENE-11536541])

HSP 1 Score: 108.227 bits (269), Expect = 2.582e-26
Identity = 89/318 (27.99%), Postives = 135/318 (42.45%), Query Frame = 1
Query:   28 LFILLCNLFVLIYSRSFN--VISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSIS--ERAQIANLKIQSNKFCEKVYNQGAD------LNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILE 951
            +  LLC +  +++ +++   V+SKRIV G D+ E ++ W ISL+      E  + CG SL+   W+LTAAHCF +                                 KY  AY G Y+  SD D    +              V  I +HP+Y  +    DIAL++L+ PI        ++ + LPS+    P   +G MC + GWG       +   +  Q A + + +   CE +Y           L  +   CAG  +     C+GDSG  L C   N W   G+ S        N PG++T V Y+++WI E
Sbjct:    1 MLRLLCVVLSILHHQAYGGPVMSKRIVGGTDSEEGEWPWQISLE-----FEGGFLCGGSLLTDSWVLTAAHCFDSMNVS-----------------------------KY-TAYLGVYQ-LSDLDNAVLR-------------GVKNITVHPDYMYEGSSGDIALIELEEPI---VFTPSIQPVCLPSQDVPLP---MGTMCWVTGWGNIKENTPLEDPQTLQKAEVGLINRTSCEAMYQSSLGYRPSIHLIQDDMICAGYKQGKIDACQGDSGGPLVCNTSNTWLQFGIVSWGLGCAEPNQPGVYTNVQYYLTWIQE 263          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Xenopus
Match: F11 (kallikrein B1 [Source:NCBI gene;Acc:100486524])

HSP 1 Score: 108.612 bits (270), Expect = 2.051e-25
Identity = 85/290 (29.31%), Postives = 124/290 (42.76%), Query Frame = 1
Query:   88 SKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVAS-ATHAELPGNYPGIFTRVSYFISWILEN 954
            + RIV G D+   ++ W +S+   +      + CG S+I  QW++TAAHCF             P +W   +G  KL                    +++ F  T+                  +II+HP+Y       DIAL+KL++PI     N+  + I LP    +   P     C + GWG     GS+S   Q A +   S + C+  Y Q     D+   CAG  +     C+GDSG  LAC     W L G+ S       PG  PG++TRVS F  WI+E+
Sbjct:  357 ANRIVGGTDSVLGEWPWQVSMHLRLTASYKKHACGGSIISNQWIVTAAHCFAMHPL--------PQMWIIYSGVVKLSN----------------ITQSTPFSETE------------------QIIIHPHYTGAGNGTDIALLKLKTPIS---FNDHQKAICLPPREPTFVLP---NSCWITGWGFTEESGSLSNILQKAEVPPISTEECQGNYEQTRI--DKKILCAGYKRGKIDSCKGDSGGPLACVVDEIWYLTGITSWGEGCARPGK-PGVYTRVSEFTDWIIEH 595          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000016164.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:9:2540736:2543374:1 gene:ENSXETG00000009594.1 transcript:ENSXETT00000016164.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 104.375 bits (259), Expect = 4.021e-25
Identity = 87/299 (29.10%), Postives = 131/299 (43.81%), Query Frame = 1
Query:   82 VISKRIVNGKDAPEDKYLWSISLK--GDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAG-ARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERA-QIANLKIQSNKFCEKVYN-------QGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWI 945
            ++  RI+ G++AP  K+ W  +L+  G  P       CG +LI ++W+LTAA                   W  R G +   ++ +  +   Y    K                DK   P S     V +II+HP+Y+   +  +IAL++L + ++   +N+    + LP  + + P    GQ C + GWG   +    S R  +   +K+ SN  C  ++N         A+L D    CAG  K G   C GD G  L C K  +W LAGV S        N PGI+TRVS +I WI
Sbjct:   31 LVRSRIMGGQEAPYGKWPWQANLRRPGYYPY------CGGTLIGEKWILTAAR------------------WWKREGVSNNTKSSFQVFVGDYNLDNK----------------DKGEQPVS-----VKRIIIHPSYREGYLNDNIALLELATKVQ---MNKVTLPVCLPDASVTFP---DGQKCSVTGWGQIMDADPPSPRVLREVEVKMMSNDRCNTLFNIPDAYGRTTANLTDTM-LCAGYAKGGRDSCNGDVGGPLVCPKDGRWYLAGVVSGGDGCGKPNRPGIYTRVSSYIKWI 277          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Mouse
Match: Klkb1 (kallikrein B, plasma 1 [Source:MGI Symbol;Acc:MGI:102849])

HSP 1 Score: 121.324 bits (303), Expect = 8.483e-30
Identity = 88/295 (29.83%), Postives = 132/295 (44.75%), Query Frame = 1
Query:   85 ISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN 969
            I+ RIV G +A   ++ W +SL+  + +   +  CG S+I +QW+LTAAHCF             P +W    G   L +E  K                               P S     + ++I+H  YK  E  +DIAL+KLQ+P+      EF + I LPS+AD+     I   C + GWG     G      Q A + +  N+ C+K Y     + ++   CAG  + G   C+GDSG  L CK   +W L G+ S        + PG++T+VS ++ WILE     +
Sbjct:  387 INARIVGGTNASLGEWPWQVSLQVKLVSQTHL--CGGSIIGRQWVLTAAHCFDGIPY--------PDVWRIYGGILSL-SEITK-----------------------------ETPSS----RIKELIIHQEYKVSEGNYDIALIKLQTPLN---YTEFQKPICLPSKADTN---TIYTNCWVTGWGYTKEQGETQNILQKATIPLVPNEECQKKYRD--YVINKQMICAGYKEGGTDACKGDSGGPLVCKHSGRWQLVGITSWGEGCARKDQPGVYTKVSEYMDWILEKTQSSD 629          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Mouse
Match: Tpsab1 (tryptase alpha/beta 1 [Source:MGI Symbol;Acc:MGI:96943])

HSP 1 Score: 113.62 bits (283), Expect = 1.817e-28
Identity = 83/297 (27.95%), Postives = 134/297 (45.12%), Query Frame = 1
Query:   97 IVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQ--SNKFCEKVYNQGADLND------EHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINK 963
            IV G++A  +K+ W +SL+ +      ++ CG SLI  QW+LTAAHC              P +        +L  +Y+ Y                                    ++VS+II HP++   +   DIAL+KL +P+    +++++  +PLP  +++ P    G +C + GWG   NG ++     +  +++    N  C+  Y++G    D      +   CAG    GH  C+GDSG  L CK  + W  AGV S        N PGI+TRV+Y++ WI   + K
Sbjct:   66 IVGGQEAHGNKWPWQVSLRAN--DTYWMHFCGGSLIHPQWVLTAAHC------------VGPDVADPNKVRVQLRKQYLYYHDHL--------------------------------MNVSQIITHPDFYIVQDGADIALLKLTNPVN---ISDYVHPVPLPPASETFP---SGTLCWVTGWGNIDNGVNLPPPFPLKEVQVPIIENHLCDLKYHKGLITGDNVHIVRDDMLCAGN--EGHDSCQGDSGGPLVCKVEDTWLQAGVVSWGEDCAQPNRPGIYTRVTYYLDWIHRYVPK 308          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Mouse
Match: Tmprss9 (transmembrane protease, serine 9 [Source:MGI Symbol;Acc:MGI:3612246])

HSP 1 Score: 114.005 bits (284), Expect = 2.998e-27
Identity = 82/291 (28.18%), Postives = 129/291 (44.33%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKK-GNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINK 963
            RIV G  A   ++ W +SL          + CGA L+ ++WLL+AAHCF            DP+ W A  G                         T     T+G+ ++           V++I  HP Y    +++D+AL++L  P+    L   +  I LP  A     P  G  C++ GWG    GGS++ + Q A +++ S + C + Y            CAG  + G   C GD+G  LAC++   +W L GV S  +     ++PG++TRV+  + WI +NI +
Sbjct:  867 RIVGGSAASLGEWPWQVSLW----LRRREHRCGAVLVAERWLLSAAHCFDI--------YGDPMQWAAFLG-------------------------TPFLSSTEGQLER-----------VARIYRHPFYNIYTLDYDVALLELAGPVRRSRL---VRPICLPGPAR----PPDGARCVITGWGSLREGGSMARQLQKAAVRVLSEQTCRRFYPVQI---SSRMLCAGFPQGGVDSCSGDAGGPLACREPSGQWVLTGVTSWGYGCGRPHFPGVYTRVAAVLGWIGQNIQE 1099          

HSP 2 Score: 98.9821 bits (245), Expect = 3.423e-22
Identity = 79/288 (27.43%), Postives = 125/288 (43.40%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWG-CKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKK-GNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILE 951
            RIV G +A   ++ W +SL+     +   + CGA++I  +WL++AAHCF   Q        DP  W A+AG+  L                          G++    + R         V +I  HP Y  D  ++D+A+++L  P+       +++   LP+     P    G+ C++ GWG  K +     E  Q A +++     C  +Y  G  L D    CAG        C+GDSG  L C++   ++ LAG+ S          PG++TRV+    WILE
Sbjct:  238 RIVGGVEAAPGEFPWQVSLR-----ENHEHFCGATIIGARWLVSAAHCFNEFQ--------DPAQWAAQAGSVHLS-------------------------GSEASAVRTR---------VLRIAKHPAYDADTADFDVAVLELARPLP---FGRYVQPACLPAATHVFP---PGKKCLISGWGYLKEDFLVKPEVLQKATVELLDQSLCSSLY--GHSLTDRM-VCAGYLDGKVDSCQGDSGGPLVCEEPSGRFFLAGIVSWGIGCAEARRPGVYTRVTRLRDWILE 469          

HSP 3 Score: 93.9745 bits (232), Expect = 2.017e-20
Identity = 79/291 (27.15%), Postives = 128/291 (43.99%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSIS-ERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKG-NKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENIN 960
            RIV G  A   +  W  SLK     +   + CGA+++  +WLL+AAHCF                             + K E    +A+ G    T    G  G         S + L + ++ LHP Y P  +++D+AL++L  P+     N++++ + LP      P   +G+ C++ GWG    G +   +  Q A++ I   K C  +YN    L D    CAG  +     C+GDSG  LAC++    + LAG+ S          PG++ R++    WIL+ ++
Sbjct:  539 RIVGGISAVSGEVPWQASLK-----EGPRHFCGATVVGDRWLLSAAHCF----------------------------NHTKVEQV--QAHLG----TVSLLGVGG---------SPVKLGLRRVALHPRYNPGILDFDVALLELAQPL---VFNKYIQPVCLPLAIHKFP---VGRKCMISGWGNMQEGNATKPDILQKASVGIIEQKMCGALYN--FSLTDRM-LCAGFLEGRVDSCQGDSGGPLACEETPGVFYLAGIVSWGIGCAQAKKPGVYARITRLKDWILKAMS 772          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Mouse
Match: Tmprss9 (transmembrane protease, serine 9 [Source:MGI Symbol;Acc:MGI:3612246])

HSP 1 Score: 114.005 bits (284), Expect = 2.998e-27
Identity = 82/291 (28.18%), Postives = 129/291 (44.33%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKK-GNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINK 963
            RIV G  A   ++ W +SL          + CGA L+ ++WLL+AAHCF            DP+ W A  G                         T     T+G+ ++           V++I  HP Y    +++D+AL++L  P+    L   +  I LP  A     P  G  C++ GWG    GGS++ + Q A +++ S + C + Y            CAG  + G   C GD+G  LAC++   +W L GV S  +     ++PG++TRV+  + WI +NI +
Sbjct:  867 RIVGGSAASLGEWPWQVSLW----LRRREHRCGAVLVAERWLLSAAHCFDI--------YGDPMQWAAFLG-------------------------TPFLSSTEGQLER-----------VARIYRHPFYNIYTLDYDVALLELAGPVRRSRL---VRPICLPGPAR----PPDGARCVITGWGSLREGGSMARQLQKAAVRVLSEQTCRRFYPVQI---SSRMLCAGFPQGGVDSCSGDAGGPLACREPSGQWVLTGVTSWGYGCGRPHFPGVYTRVAAVLGWIGQNIQE 1099          

HSP 2 Score: 98.9821 bits (245), Expect = 3.423e-22
Identity = 79/288 (27.43%), Postives = 125/288 (43.40%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWG-CKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKK-GNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILE 951
            RIV G +A   ++ W +SL+     +   + CGA++I  +WL++AAHCF   Q        DP  W A+AG+  L                          G++    + R         V +I  HP Y  D  ++D+A+++L  P+       +++   LP+     P    G+ C++ GWG  K +     E  Q A +++     C  +Y  G  L D    CAG        C+GDSG  L C++   ++ LAG+ S          PG++TRV+    WILE
Sbjct:  238 RIVGGVEAAPGEFPWQVSLR-----ENHEHFCGATIIGARWLVSAAHCFNEFQ--------DPAQWAAQAGSVHLS-------------------------GSEASAVRTR---------VLRIAKHPAYDADTADFDVAVLELARPLP---FGRYVQPACLPAATHVFP---PGKKCLISGWGYLKEDFLVKPEVLQKATVELLDQSLCSSLY--GHSLTDRM-VCAGYLDGKVDSCQGDSGGPLVCEEPSGRFFLAGIVSWGIGCAEARRPGVYTRVTRLRDWILE 469          

HSP 3 Score: 93.9745 bits (232), Expect = 2.017e-20
Identity = 79/291 (27.15%), Postives = 128/291 (43.99%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSIS-ERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKG-NKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENIN 960
            RIV G  A   +  W  SLK     +   + CGA+++  +WLL+AAHCF                             + K E    +A+ G    T    G  G         S + L + ++ LHP Y P  +++D+AL++L  P+     N++++ + LP      P   +G+ C++ GWG    G +   +  Q A++ I   K C  +YN    L D    CAG  +     C+GDSG  LAC++    + LAG+ S          PG++ R++    WIL+ ++
Sbjct:  539 RIVGGISAVSGEVPWQASLK-----EGPRHFCGATVVGDRWLLSAAHCF----------------------------NHTKVEQV--QAHLG----TVSLLGVGG---------SPVKLGLRRVALHPRYNPGILDFDVALLELAQPL---VFNKYIQPVCLPLAIHKFP---VGRKCMISGWGNMQEGNATKPDILQKASVGIIEQKMCGALYN--FSLTDRM-LCAGFLEGRVDSCQGDSGGPLACEETPGVFYLAGIVSWGIGCAQAKKPGVYARITRLKDWILKAMS 772          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Mouse
Match: Prss28 (protease, serine 28 [Source:MGI Symbol;Acc:MGI:2149951])

HSP 1 Score: 108.997 bits (271), Expect = 4.188e-27
Identity = 90/299 (30.10%), Postives = 130/299 (43.48%), Query Frame = 1
Query:   97 IVNGKDAPEDKYLWSISLKG-DIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKI--QSNKFCEKVYNQGADLNDEHE--------FCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENIN 960
            IV G+  P  K+ W +SL+      +  V+ CG S+I  QW+LTAAHC  +          DP ++  + G   L  E                +E                      L++S+II+HP+Y      +D+ALM+L +      L     V P+    DS  +    Q C L+GWG       +    Q+  +KI  Q NK C++ Y + +  +DEH+         CAG   SG G C GDSG  L C K NKW   GV S    +   N P IF+RV   ++WI ++I 
Sbjct:   31 IVGGQCTPPGKWPWQVSLRMYSYEVNSWVHICGGSIIHPQWILTAAHCIQS-------QDADPAVYRVQVGEVYLYKE----------------QEL---------------------LNISRIIIHPDYNDVSKRFDLALMQLTA-----LLVTSTNVSPVSLPKDSSTFDSTDQ-CWLVGWGNLLQRVPLQPPYQLHEVKIPIQDNKSCKRAYRKKS--SDEHKAVAIFDDMLCAGT--SGRGPCFGDSGGPLVCWKSNKWIQVGVVSKG-IDCSNNLPSIFSRVQSSLAWIHQHIQ 274          
BLAST of Peptidase S1 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q2KJ63|KLKB1_BOVIN (Plasma kallikrein OS=Bos taurus OX=9913 GN=KLKB1 PE=2 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 2.079e-29
Identity = 90/295 (30.51%), Postives = 131/295 (44.41%), Query Frame = 1
Query:   67 SRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILE 951
            SR       RIV G +A   ++ W +SL+  +      + CG S+I +QW+LTAAHCF       LL S    +W    G   L       E   E ++                              + +II+HPNYK  E   DIAL+KL++P+      +  + I LPS+ D+KP   +   C + GWG     G I    Q AN+ + SN+ C+K Y        +   CAG  + G   C+GDSG  L C+    W L G+ S          PG++T+V+ ++ WILE
Sbjct:  382 SRVCTTKRTRIVGGTNASWGEWPWQVSLQ--VKQRAQSHLCGGSIIGRQWVLTAAHCF-----DGLLLSN---IWRIYGGILNLS------EITTETSFS----------------------------QIKEIIVHPNYKISEGSHDIALIKLEAPLN---FTDLQKAICLPSKDDTKP---VYTDCWITGWGFTEEKGKIQNTLQKANIPLISNEECQKSYRDYKI--TKQMICAGYKEGGKDACKGDSGGPLVCQHEETWHLVGITSWGEGCARREQPGVYTKVAEYVDWILE 624          
BLAST of Peptidase S1 domain-containing protein vs. UniProt/SwissProt
Match: sp|P26262|KLKB1_MOUSE (Plasma kallikrein OS=Mus musculus OX=10090 GN=Klkb1 PE=1 SV=2)

HSP 1 Score: 121.324 bits (303), Expect = 5.934e-29
Identity = 88/295 (29.83%), Postives = 132/295 (44.75%), Query Frame = 1
Query:   85 ISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN 969
            I+ RIV G +A   ++ W +SL+  + +   +  CG S+I +QW+LTAAHCF             P +W    G   L +E  K                               P S     + ++I+H  YK  E  +DIAL+KLQ+P+      EF + I LPS+AD+     I   C + GWG     G      Q A + +  N+ C+K Y     + ++   CAG  + G   C+GDSG  L CK   +W L G+ S        + PG++T+VS ++ WILE     +
Sbjct:  387 INARIVGGTNASLGEWPWQVSLQVKLVSQTHL--CGGSIIGRQWVLTAAHCFDGIPY--------PDVWRIYGGILSL-SEITK-----------------------------ETPSS----RIKELIIHQEYKVSEGNYDIALIKLQTPLN---YTEFQKPICLPSKADTN---TIYTNCWVTGWGYTKEQGETQNILQKATIPLVPNEECQKKYRD--YVINKQMICAGYKEGGTDACKGDSGGPLVCKHSGRWQLVGITSWGEGCARKDQPGVYTKVSEYMDWILEKTQSSD 629          
BLAST of Peptidase S1 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9N2D1|TRYT_PIG (Tryptase OS=Sus scrofa OX=9823 GN=MCT7 PE=2 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 2.799e-28
Identity = 88/300 (29.33%), Postives = 136/300 (45.33%), Query Frame = 1
Query:   97 IVNGKDAPEDKYLWSISLKGDIPTDEA-VYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLK--IQSNKFCEKVYNQGADLND------EHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN 969
            IV GK+AP  K+ W +SL+     D+   + CG SLI  QW+LTAAHCF                     G  K +  Y++ +   +  Y   Y++                      L VS+II+HPNY  +    DIAL++L+ P+    L+  ++ + LP  +++ P    G  C + GWG   +G  +     +  ++  I  N  C+  Y+ G    D      +   CAG    GH  C+GDSG  L C+    W  AGV S        N PGI+TRV++++ WI + I +++
Sbjct:   31 IVGGKEAPGHKWPWQVSLRC---LDQYWKHFCGGSLIHPQWVLTAAHCF---------------------GPEKADPLYIRVQLGEQHLY---YQDRL--------------------LLVSRIIVHPNYYDEVNGADIALLELEDPVN---LSSHVQPVTLPPASETFP---KGTRCWVTGWGDVHSGWPLPPPYPLKQVRVPIVENSECDMQYHLGLSTGDNIPIVRDDMLCAGS--EGHDSCQGDSGGPLVCRVNGTWLQAGVVSWGEGCALPNRPGIYTRVTHYLDWIHQCIPRES 275          
BLAST of Peptidase S1 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q02844|TRYB1_MOUSE (Tryptase OS=Mus musculus OX=10090 GN=Tpsab1 PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 5.927e-28
Identity = 83/297 (27.95%), Postives = 133/297 (44.78%), Query Frame = 1
Query:   97 IVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQ--SNKFCEKVYNQGADLND------EHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINK 963
            IV G++A  +K+ W +SL+ +      ++ CG SLI  QW+LTAAHC              P +        +L  +Y+ Y                                    + VS+II HP++   +   DIAL+KL +P+    +++++  +PLP  +++ P    G +C + GWG   NG ++     +  +++    N  C+  Y++G    D      +   CAG    GH  C+GDSG  L CK  + W  AGV S        N PGI+TRV+Y++ WI   + K
Sbjct:   29 IVGGQEAHGNKWPWQVSLRAN--DTYWMHFCGGSLIHPQWVLTAAHC------------VGPDVADPNKVRVQLRKQYLYYHDHL--------------------------------MTVSQIITHPDFYIVQDGADIALLKLTNPVN---ISDYVHPVPLPPASETFP---SGTLCWVTGWGNIDNGVNLPPPFPLKEVQVPIIENHLCDLKYHKGLITGDNVHIVRDDMLCAGN--EGHDSCQGDSGGPLVCKVEDTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPK 271          
BLAST of Peptidase S1 domain-containing protein vs. UniProt/SwissProt
Match: sp|P14272|KLKB1_RAT (Plasma kallikrein OS=Rattus norvegicus OX=10116 GN=Klkb1 PE=1 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.556e-28
Identity = 87/292 (29.79%), Postives = 131/292 (44.86%), Query Frame = 1
Query:   85 ISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENIN 960
            I+ RIV G ++   ++ W +SL+  + +   +  CG S+I +QW+LTAAHCF             P +W    G   L                    E ++           + P S     + ++I+H  YK  E  +DIAL+KLQ+P+      EF + I LPS+AD+     I   C + GWG     G      Q A + +  N+ C+K Y     +  +   CAG  + G   C+GDSG  L CK   +W L G+ S          PG++T+V+ +I WILE I 
Sbjct:  387 INARIVGGTNSSLGEWPWQVSLQVKLVSQNHM--CGGSIIGRQWILTAAHCFDGIPY--------PDVWRIYGGILNLS-------------------EITN-----------KTPFS----SIKELIIHQKYKMSEGSYDIALIKLQTPLN---YTEFQKPICLPSKADTN---TIYTNCWVTGWGYTKERGETQNILQKATIPLVPNEECQKKYRD--YVITKQMICAGYKEGGIDACKGDSGGPLVCKHSGRWQLVGITSWGEGCARKEQPGVYTKVAEYIDWILEKIQ 626          
BLAST of Peptidase S1 domain-containing protein vs. TrEMBL
Match: A0A074Z675 (Peptidase S1 domain-containing protein OS=Opisthorchis viverrini OX=6198 GN=T265_15173 PE=3 SV=1)

HSP 1 Score: 224.557 bits (571), Expect = 1.632e-67
Identity = 130/328 (39.63%), Postives = 183/328 (55.79%), Query Frame = 1
Query:   46 NLFVL--IYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLA-AQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKW------------------DKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQS--PIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENIN 960
             LFVL  + + S   + KRI+NG DA    + W++S+KG  PT  ++  CGA++I +QWLLTAAHCF   A+ KS+LN   P  WHA+AG  K+    +  EH   +   G     SD D T   +                  D+  A +    +H  KIILHP Y P+++E+DIAL+KL++  PI+ K L        LP+   +  WP +G  C  +GWGC+   G  +   Q+  L++ S+  C ++YN GA LN+ HEFCAG Y+S  G+C GDSG GL      +  + GVASATHA  P +YPG+FTRVS F  WI + ++
Sbjct:    6 TLFVLLEVAAGSSIRVDKRIINGYDAKTGDHPWAVSIKGIHPTTLSITYCGATIIAEQWLLTAAHCFWTDARRKSMLN---PGYWHAQAGHTKI---VLGEEHTKREIIVG----ASDTDETGSNFLQNLFESLKKLILFKTSTDEAVADDKLWHVHFQKIILHPKYAPNDLEYDIALVKLRAALPIDGKKL----AAAKLPAGLTTGDWPPVGTQCTFVGWGCQKYQGEPTANLQVVALRVVSHDACTEMYNHGAGLNEHHEFCAGYYQSNVGICAGDSGGGLIQMYERQVVVVGVASATHARQPQSYPGLFTRVSAFTDWIEKTVD 319          
BLAST of Peptidase S1 domain-containing protein vs. TrEMBL
Match: A0A4S2MLJ2 (Peptidase S1 domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_000382 PE=3 SV=1)

HSP 1 Score: 218.779 bits (556), Expect = 2.791e-65
Identity = 126/324 (38.89%), Postives = 178/324 (54.94%), Query Frame = 1
Query:   31 FILLCNLFVL--IYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLA-AQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGT-----------DGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENIN 960
             IL   LFVL  + + S   + KRI+NG DA    + W++S+KG  P   ++  CGA++I  QWLLTAAHCF   A+ +S+L    P  WHA+AG  K+       + +      G  E  S+F                  D+    +    +H  KIILHP Y PD++E+DIAL+KL++P+      + L    LP+   +  WP +G  C  +GWGC+   G  S   Q+  L++ S+  C ++YN GA LN+ HEFCAG YKS  G+C GDSG GL      +  + GVASATHA+ P +YPG+FTRVS F  WI + ++
Sbjct:    1 MILHWTLFVLLGVAAGSSIRVDKRIINGYDAKGGDHPWAVSIKGIHPRTLSITYCGATIIADQWLLTAAHCFWTDARRRSMLI---PGYWHAQAGHTKIVLGEEHTDREIIVGSSGIDEVGSNFLQNLFENLKKLILFKTPSDQAITDDKLWHIHFEKIILHPKYLPDDLEYDIALVKLRAPLPID--GKTLTAAKLPAGLTTGDWPPVGTQCTFVGWGCQRYQGEPSADLQVVALRVVSHDACTEMYNNGAGLNEHHEFCAGYYKSNVGICAGDSGGGLIQVFERQVVVVGVASATHAKQPQSYPGLFTRVSAFTDWIEKTVD 319          
BLAST of Peptidase S1 domain-containing protein vs. TrEMBL
Match: A0A4E0RH51 (Subfamily S1A unassigned peptidase (S01 family) OS=Fasciola hepatica OX=6192 GN=D915_002871 PE=3 SV=1)

HSP 1 Score: 218.009 bits (554), Expect = 6.772e-65
Identity = 118/296 (39.86%), Postives = 168/296 (56.76%), Query Frame = 1
Query:   88 SKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVK--YEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN 969
            SKRI+NG DA E +Y W+++LKG  P    +  CGA++IDK+WLL+AAHCF   ++K+ L  T P  W  +AG  K+E   V+    H  E+ +    +            D +++  S     V  II+HP Y+PD++E+DIAL+KL +P+    +   +    LP + +S  WP    +C  +GWGC+      SE+AQ+  LK+  +  C  +Y   A LN EHEFCAG Y+S  G+C GDSGSGL         + GVASATHA+ P  +PG+FTRVS FI WI   +   +
Sbjct:   23 SKRIINGYDAKEKEYPWAVALKGIHPQTRLITYCGATIIDKRWLLSAAHCFWNTKKKNHL--TKPENWIVQAGKAKIELGDVEPNVSHDKEEKHSDVLKTIVGIFRRLFPSDSKQSGNSSTYYKVQNIIVHPQYQPDDLEYDIALLKLLTPLPS--MESGIAKAYLPVDGNSDKWPPEDALCTFVGWGCRVKDKGPSEKAQVVALKVTPHFICSLMYQHAAGLNAEHEFCAGFYQSNVGICPGDSGSGLIYVDEGLPYVVGVASATHAKEPEGFPGLFTRVSTFIEWITSTMETTD 314          
BLAST of Peptidase S1 domain-containing protein vs. TrEMBL
Match: A0A5J4P102 (Peptidase S1 domain-containing protein OS=Paragonimus westermani OX=34504 GN=DEA37_0014782 PE=3 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 1.119e-64
Identity = 129/365 (35.34%), Postives = 191/365 (52.33%), Query Frame = 1
Query:   34 ILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKS--LLNSTDPILWHARAGARKLET--------EYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQS--PIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLC-----------------------------------------EGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN 969
            ++L  +FVL      + I KR++NG D  E++Y W++S+KG  P    +  CG+++I  +WLLTAAHCF A+ +K   L+N   P  WHA+AG+ ++E         E ++   +  + +    +   ++     K  +     +   LH  KIILHP Y+PDE+E DIAL+KL+   P+ ++ ++    +  LPS   +  WP +   C  +GWGC+ N G  S RAQ+  LK+  N  C ++YN GA LN EHEFCAG YKS  G+C                                         +GDSGSGL      +  + GVASATHAE P ++PG+FTRV+ F  WI E I+K++
Sbjct:    8 VVLGTIFVL---GECSEIGKRVINGYDVQENQYPWAVSIKGIHPITRTITFCGSTIIADRWLLTAAHCFWASMKKKSYLMN---PENWHAQAGSAEIELGDRHTDREEVIETSAQPSETFFRLLQRLFEYLQRKSKHQE----NTIYHLHAEKIILHPKYRPDELENDIALVKLKGTLPLAKRSVS----LAKLPSGWKTSDWPPVSSTCTFVGWGCQINNGPPSSRAQVIALKVLPNPVCSQIYNHGAGLNAEHEFCAGYYKSKVGICAALLQSNYVYFVVTAIANLTVICFFPSVLNPTNNNACSLRKQGDSGSGLIYLYKRQPVVVGVASATHAERPQSFPGLFTRVASFTDWIQETIHKES 358          
BLAST of Peptidase S1 domain-containing protein vs. TrEMBL
Match: G7Y3P9 (Serine protease 33 OS=Clonorchis sinensis OX=79923 GN=CLF_100549 PE=3 SV=1)

HSP 1 Score: 216.468 bits (550), Expect = 2.595e-64
Identity = 127/324 (39.20%), Postives = 182/324 (56.17%), Query Frame = 1
Query:   31 FILLCNLFVL--IYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLA-AQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDF------DGTDGKWDKRRAPESYLD-----LHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENIN 960
             +L   LFV   + + S   + KRI+NG DA +  + W++S+KG  P+  ++  CGA++I  QWLLTAAHCF    + +S+LN   P  WHA+AG  ++         +      G  E  S+F      +       K  A E+  D     +H +KIILHP Y PD++E+DIAL+KL++P+      + L V  LP+   +  WP IG  C  +GWGC+   G  +   Q+  L++ S + C ++YN GA LN  HEFCAG YKS  G+C GDSG GL      +  + GVASATHA+ P +YPG+FTRVS F  WI + ++
Sbjct:    1 MVLQWTLFVFLGVAAGSSIRVDKRIINGYDAKKGDHPWAVSIKGIHPSTLSITYCGATIIADQWLLTAAHCFWTDVRRRSMLN---PKYWHAQAGHTQIALGEEHTNREIIVGTNGIDEAGSNFLQNLFENLKKLILYKTSADEAITDDQLWHVHFAKIILHPKYVPDDLEYDIALIKLRAPLPID--GKKLAVAKLPAGLTTGDWPPIGTQCTFVGWGCQRYQGEPTANLQVVALRVVSYEACTEMYNHGAGLNKHHEFCAGYYKSNVGICAGDSGGGLIQVFEQQVVVVGVASATHAKQPQSYPGLFTRVSAFTKWIEKTVD 319          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000033802.1 (pep primary_assembly:Astyanax_mexicanus-2.0:3:21891941:21894733:1 gene:ENSAMXG00000030254.1 transcript:ENSAMXT00000033802.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 116.701 bits (291), Expect = 6.346e-30
Identity = 85/294 (28.91%), Postives = 130/294 (44.22%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLE--VIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLAC-KKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQ 966
            +IV G DA    + W +SL+     +   + CGASL+  +WLL+AAHCF   Q+   + S D   W A  G R + T                                  A  +     + +I+ H  Y     ++DIAL++L +P+   + N+ ++   +P PS A +      G  C + GWG  T    ++   Q A + I S+K C K+Y+   D       CAG  + G   C+GDSG  L C ++G +W LAG+ S        N PG++T+V  F  WI +    Q
Sbjct:   52 KIVGGTDAQAGSWPWQVSLQ----MERYGHVCGASLVGTRWLLSAAHCF---QDSDAIYS-DARAWRAFLGMRVMNT----------------------------------ASNAAATRQIRRIVQHAQYDQFTSDYDIALLELSAPV---FFNDLVQPVCVPAPSHAFTS-----GTTCFVTGWGVLTEE-ELATLLQEATVSIISHKTCNKMYD---DAVTPRMICAGNIQGGVDACQGDSGGPLVCLERGRRWFLAGIVSWGEGCARQNRPGVYTQVIKFTDWIHQQTKGQ 291          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000014374.2 (chymotrypsinogen A-like [Source:NCBI gene;Acc:103033709])

HSP 1 Score: 109.383 bits (272), Expect = 1.718e-27
Identity = 80/289 (27.68%), Postives = 124/289 (42.91%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWG-CKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENI 957
            RIVNG++A    + W +SL+         + CG SL+++ W+LTAAHC +      +L                                              G+ D+    E+   L + K+I HP+Y  + I  D+ L+KL S  +   LN  +  + LP+ +D       G  C+  GWG  + N  +   R Q   L + +N  C++ +  G+ +      CAG   +G   C GDSG  L C+KG  W L GV S   +    + PG++ RV+ F SWI + I
Sbjct:   33 RIVNGEEAVPHSWPWQVSLQ----DSTGWHFCGGSLLNQNWVLTAAHCGVRTSHYVIL----------------------------------------------GEHDRSSNAEAIQTLRIGKVIQHPSYNSNTINNDVTLIKLASTAQ---LNTRVSPVCLPASSDDY---AGGLKCVTTGWGLTRYNAANTPPRLQQTALPLLTNSNCQRYW--GSSITSVM-VCAGA--AGSSSCMGDSGGPLVCQKGGVWNLVGVVSWGSSTCSTSSPGVYARVTSFRSWIDQTI 260          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000014359.2 (pep primary_assembly:Astyanax_mexicanus-2.0:8:20902992:20905615:-1 gene:ENSAMXG00000013952.2 transcript:ENSAMXT00000014359.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 109.383 bits (272), Expect = 2.824e-27
Identity = 80/289 (27.68%), Postives = 123/289 (42.56%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWG-CKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENI 957
            RIVNG++A    + W +SL+         + CG SL+++ W++TAAHC +      +L                                              G+ D+    E+   L + K+I HP+Y  + I  DI L+KL S  +   LN  +  + LP+ +D       G  C+  GWG  + N  +   R Q   L + +N  C++ +  G  +      CAG   +G   C GDSG  L C+KG  W L GV S   +    + PG++ RV+ F SWI + I
Sbjct:   61 RIVNGEEAVPHSWPWQVSLQ----DSTGWHFCGGSLVNQNWVVTAAHCGVRTSHYVIL----------------------------------------------GEHDRSSNAEAIQTLRIGKVIQHPSYNSNTINNDITLVKLASTAQ---LNTRVSPVCLPASSDDY---AGGLKCVTTGWGLTRYNAANTPPRLQQTALPLLTNSNCQRYW--GTSITSVM-ICAGA--AGASSCMGDSGGPLVCQKGGVWNLVGVVSWGSSTCSTSSPGVYARVTSFRSWIDQTI 288          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Cavefish
Match: ctrb1 (chymotrypsin A-like [Source:NCBI gene;Acc:103027485])

HSP 1 Score: 105.145 bits (261), Expect = 6.914e-26
Identity = 87/321 (27.10%), Postives = 135/321 (42.06%), Query Frame = 1
Query:   19 MKRLFILLCNLFV-LIYSRSFNVIS------KRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWG-CKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENI 957
            M  L+IL C  F    Y      I+       RIVNG++A    + W +SL+         + CG SLI++ W++TAAHC +    + +L                                              G+ D+    ES   + V K+  HP Y    I  DI L+KL +P +   +N  +  + + + +D+ P    G  C+  GWG  K N      + Q A+L + +N  C++ +  G+ ++D    CAG   SG   C GDSG  L C+K   WTL G+ S        + PG++ RV+   SW+ + I
Sbjct:    1 MAFLWILSCLAFASAAYGCGIPAITPVISGYSRIVNGEEAVPHSWPWQVSLQ----DYTGFHFCGGSLINEWWVVTAAHCNVRTSHRVIL----------------------------------------------GEHDRSSNAESIQTMSVGKVFKHPQYNGFTINNDILLIKLATPAQ---MNPRVSPVCVAATSDNFPG---GMKCVTSGWGLTKHNAPDTPAKLQQASLPLLTNDDCKRFW--GSQISDVM-ICAGA--SGASSCMGDSGGPLVCQKSGAWTLVGIVSWGSGTCTTSMPGVYARVTKLRSWMDQTI 260          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000049166.1 (serine protease 56 [Source:NCBI gene;Acc:103021696])

HSP 1 Score: 108.997 bits (271), Expect = 1.216e-25
Identity = 85/300 (28.33%), Postives = 125/300 (41.67%), Query Frame = 1
Query:   70 RSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKK--GNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINK 963
            R+      RIV G  AP   + W ++L+ D         CG  L+D  W+LTAAHCF  ++  S         W A  G                           +FD T      +  PE  + L V+++I HP + P     DIAL++L SP+    L++ +  + LPS  +    P  G  C++ GWG     G  ++    A + + S   C+     G +L     FCAG    G   C+GDSG  L  +     ++ L G+ S          PG++TRV+ F  WIL  I K
Sbjct:   89 RNLTQPRSRIVGGSPAPPGSWPWLVNLQLD-----GALMCGGVLVDSSWVLTAAHCFAGSRSDSY--------WTAVVG---------------------------EFDIT------KTDPEEQV-LKVNRVITHPKFNPKTFNNDIALVELTSPV---VLSDQVTPVCLPSGIE----PPTGTPCLVAGWGSLYEDGPAADVVMEAKVPLLSQGTCKSAL--GKELLTNTMFCAGYLSGGIDSCQGDSGGPLIFQDRITGRFQLYGITSWGDGCGEKGKPGVYTRVTAFTDWILSEIQK 332          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002258.1 (pep scaffold:Pmarinus_7.0:GL487751:502724:507790:1 gene:ENSPMAG00000002029.1 transcript:ENSPMAT00000002258.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 100.138 bits (248), Expect = 1.056e-24
Identity = 81/294 (27.55%), Postives = 126/294 (42.86%), Query Frame = 1
Query:   91 KRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHC-FLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGC-KTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQ 966
             R++NG++A    + W +SL+     ++  + CG S+ID  W++TAAHC F  A  + ++                                              G+ DK    E+   L+V K I HP Y  + I  DI L+KL+ P    +  + +  I LP   D+      G    + GWG  + N    S   Q   L + S + C+K +  G+ + D    CAG   +GH  C GDSG  L  +K   W LAG+ S  ++    + P ++TRV+ F+ WI E + K 
Sbjct:   32 SRVINGEEAVPGSWPWQVSLQ----YEDGFHFCGGSIIDPYWVITAAHCDFKPANHRIIV----------------------------------------------GEHDKSSNVENIQILYVEKAITHPLYDANTINNDILLLKLKEP---AFFGDTVSPICLPRATDTF---SPGTHAAVTGWGVTRWNANYGSSTLQQTVLPLISTEDCKKWW--GSYIMDPM-ICAGA--NGHSTCMGDSGGPLTVQKDGMWVLAGLTSWGNSRCDTSVPVVYTRVTTFVDWIAETMAKN 264          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002946.1 (pep scaffold:Pmarinus_7.0:GL477988:107792:112981:-1 gene:ENSPMAG00000002665.1 transcript:ENSPMAT00000002946.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 99.7525 bits (247), Expect = 1.425e-24
Identity = 77/290 (26.55%), Postives = 119/290 (41.03%), Query Frame = 1
Query:   91 KRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGS-ISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENI 957
             RIV G++A    + W +SL+     +   + CG SLID  W++TAAHC +                      R  E   +  EH                       D+  + E    + ++K+I HP +       D+AL+KL  P +   + + +  + L S +D+ P    G  C+  GWG   N  S    R Q   L I +   C++++     + D    CAG   +G   C  DSG  L CKK   W L G+ S   +    + P ++TRV+   SWI + I
Sbjct:   34 SRIVKGEEAMPGSWPWQVSLQ----NNTGFHFCGGSLIDPYWIVTAAHCKI----------------------RPSEHRVILGEH-----------------------DRSSSSEKVQTMTIAKVINHPQWDDVSFNNDVALIKLPQPAQ---MTDGVSAVCLSSTSDNVP---AGLNCVTSGWGITNNFDSDTPPRLQQTALAIVATPTCQQLFASMNKITDVM-ICAGG--AGASSCRCDSGGPLVCKKNGAWNLVGIVSWGSSVCSTSMPVVYTRVTAIRSWIDQTI 265          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005493.1 (pep scaffold:Pmarinus_7.0:GL476632:243920:250733:-1 gene:ENSPMAG00000004970.1 transcript:ENSPMAT00000005493.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 98.9821 bits (245), Expect = 2.198e-24
Identity = 76/290 (26.21%), Postives = 120/290 (41.38%), Query Frame = 1
Query:   91 KRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGS-ISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENI 957
             RIVNG++A    + W +SL+    +    + CG SLID  W++TAAHC ++  E  ++                                              G+ D+  + E    + ++K I HP +    I  D+AL+KL  P +   + + +  + L S +D+ P    G  C+  GWG   N  S    R Q   L I +   C+++      + D    CAG   +G   C  DSG  L C+K   W L G+ S  ++      P ++TRV+   SWI + I
Sbjct:   25 SRIVNGEEAMPGSWPWQVSLQ----SKTGFHFCGGSLIDPYWVVTAAHCGISPSEHRVI---------------------------------------------LGEHDRSSSSEKVQTMTIAKAINHPQWDNMSINNDVALIKLSQPAQ---MTDGVSAVCLSSTSDNFP---PGLNCVTSGWGITNNFDSDTPPRLQQTALAIVATPTCQQLLANMNKITDVM-ICAGG--AGATSCRRDSGGPLVCEKDGAWNLVGIVSWGNSSCSTRIPAVYTRVTAIRSWIDQTI 256          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Sea Lamprey
Match: tmprss3a (transmembrane serine protease 3a [Source:ZFIN;Acc:ZDB-GENE-070912-70])

HSP 1 Score: 92.4337 bits (228), Expect = 2.792e-21
Identity = 73/288 (25.35%), Postives = 117/288 (40.62%), Query Frame = 1
Query:   88 SKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPD-EIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGC-KTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWI 945
            + RIV G+DA    + W +SL+ +       + CG +L+   W+L+AAHCF       + N TD                                  TS+  G+                 +SKII +  Y  +   ++DIAL+KL  P+    ++  +    LP+        ++G  C + GWG  K + G  S   Q   + I S + C    +    +      CAG  + G   C+GDSG  L C+ G  W + G  S        N+PG+++RV+ ++ WI
Sbjct:  120 TSRIVGGQDAQLGSWPWQVSLQFN-----GQHTCGGTLLTPTWVLSAAHCFQGYLGPDMWNVTD---------------------------------LTSNLAGS-----------------ISKIITNSQYVDNTNNDYDIALVKLSKPLS---MSNTIGPACLPNYQTDF---QVGMNCWITGWGATKVSAGPASTTLQEVMVPILSTETCNAANSYNGQITSRM-VCAGFMQGGQDACQGDSGGPLVCQTGTTWFVVGATSFGEGCALTNFPGVYSRVTAYLDWI 345          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001315.1 (pep scaffold:Pmarinus_7.0:GL479305:249343:254401:-1 gene:ENSPMAG00000001180.1 transcript:ENSPMAT00000001315.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 90.5077 bits (223), Expect = 7.891e-21
Identity = 81/290 (27.93%), Postives = 119/290 (41.03%), Query Frame = 1
Query:   82 VISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKT-NGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLC-EGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWI 945
            ++  RIV G+ A    + W  SL   +  +   + CG SLI  +W+LTAAHC      +    S     W    G   L+ E    +H+                                   + +I++H  Y   E ++DIAL++L SP+    L E ++ + +P+ + +      GQMC + GWG    N  S+S   Q   L + S   C   Y    D       CAG    G   C +GDSG  L C+    W LAG+ S        N PG++TRV+ F  WI
Sbjct:   84 MVKSRIVGGESALAGAWPWQASLWSGLTKN---HHCGGSLIHPKWVLTAAHCVYGKNCRVEALSD----WTLFFGKLLLDKEGPMEQHR----------------------------------SIRQIVVHRQYVSSENDYDIALLELDSPVN---LTEQVQTVCMPAASHAF---LPGQMCWVSGWGTVIGNEFSLSNNMQEVQLPLISQSDCIGSY----DHATPRMLCAGYPDGGKDACQQGDSGGPLVCEDRGSWFLAGLVSWGVGCGLVNKPGVYTRVTAFTKWI 322          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Nematostella
Match: EDO33459 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SS64])

HSP 1 Score: 120.168 bits (300), Expect = 5.627e-32
Identity = 85/284 (29.93%), Postives = 123/284 (43.31%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWI 945
            R++NG DA    + W ISL+      +  + CG SLID +W+LTAAHC    +        +P  +    GA +L                         DGT    +K         + +SKI  H  Y    +  D+AL+KL   +    L++ +  + LPS   S   P  G  C + GWG    GGS +   Q A+L + S+  C+        ++     CAG    G G C+GDSG    C++G KW L G  S  H     ++  +F RV+ FISWI
Sbjct:    2 RVINGVDATAHAWPWQISLRMMSKKGDDYHFCGGSLIDSEWVLTAAHCVAGIR--------NPRRYSVYVGAHEL-------------------------DGTTQVEEK---------ISISKIYSHEKYSSSLLTSDVALIKLSKAVS---LSKHVNTVCLPSGLSSDEAPA-GSKCFITGWGRMVAGGSGANTLQQADLLVASHSDCQARMGYMLSVDKATMICAGS--QGKGGCQGDSGGPFVCEEGGKWVLRGAVSWGHVNCLTDHYTVFARVNSFISWI 237          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Nematostella
Match: EDO39625 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9K6])

HSP 1 Score: 106.301 bits (264), Expect = 9.271e-27
Identity = 93/317 (29.34%), Postives = 135/317 (42.59%), Query Frame = 1
Query:   37 LLCNLFVLIYSRSFNVI----SKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNY-KPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHE--FCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHA-ELPGNYPGIFTRVSYFISWILENINK 963
            L+  L VLI+++         + RIV G  A    + W   L+    T      CG SLI  QW+LTA HC    Q  S++          R GAR+     V  E  Y                                  V+K+I HP+Y KP     DIAL+KL  P+      + +  + LP E D  P P  G+ C + GWG  ++GGS  +  Q  ++ I+S   C+  Y      N  H+   CAG  K G   C+GDSG  + C+ G ++ + G  S  +    PG Y G++ +V Y + WI + + K
Sbjct:    3 LILPLKVLIFAQRLGSCGIRPNTRIVGGTAAKHGDWPWQAQLR----TTSGFPYCGGSLIAPQWILTATHCVERKQASSIV---------IRLGARR-RVATVGTEKDYI---------------------------------VTKVITHPSYHKPKTYSHDIALLKLDKPV---LYTKNIHPVCLP-ELD--PEPVDGKHCWVTGWGRLSSGGSTPDYLQQVSVPIRSRARCDSSYP-----NKIHDSMICAGIDKGGIDACQGDSGGPMVCENGGRFYIHGATSWGYGCAAPGLY-GVYAKVKYLLPWIKDEMAK 260          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Nematostella
Match: EDO41035 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5M4])

HSP 1 Score: 103.605 bits (257), Expect = 6.357e-26
Identity = 77/286 (26.92%), Postives = 118/286 (41.26%), Query Frame = 1
Query:   88 SKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWI 945
            + RIV G  A    + W   L+    T      CG SLI   W+LTAAHC  + +   ++        H+R G+   + +                                       D+ VS II H NY    +  DIAL+KL +P +    N  +  + LP   +++  P  G  C + GWG  ++GGS  +  Q A++ + S   CE+ Y     ++D    CAG    G   C+GDSG  L C+ G K+ L G  S  +        G++  V Y ++W+
Sbjct:   10 TSRIVGGAAANPGDWPWQAQLR----TTTGFPYCGGSLIHSNWVLTAAHCISSKRPAEVVVRLGA---HSRLGSLTTDMQ---------------------------------------DIKVSAIITHSNYNSRTMYNDIALLKLATPAQT---NSKVGFVCLP---ETEASPSDGTTCWITGWGTLSSGGSPPDILQEASVPVVSRARCEQAYTDPNQIHDSM-LCAGLDNGGVDTCQGDSGGPLVCESGGKFVLHGATSWGYGCAAKGKFGVYANVKYLLTWV 242          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Nematostella
Match: EDO44208 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RW61])

HSP 1 Score: 102.834 bits (255), Expect = 8.499e-26
Identity = 82/287 (28.57%), Postives = 121/287 (42.16%), Query Frame = 1
Query:   94 RIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSIS--ERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKS-GHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWI 945
            RI+NG++A    + W ISL+G        + CG SLI  +W++TAAHC        +  + +P  +    GA K                                  KR +      L +S+II HP Y    I  DIAL++L +P++     +   V       D +P P  G+ C + GWG   NG +    +  Q A L I S++ C + Y    D++     CAG+ +S   G C GDSG  L C+    W L G  S      P  +  +F RV+ +  WI
Sbjct:    2 RIINGQNAQPHSWPWQISLRGR----SGFHTCGGSLISDRWVVTAAHC--------IHRNKNPGGYTVVVGAHK----------------------------------KRGSTSVQQSLRLSQIIEHPKYNDRRIVNDIALIELATPVQFDSAGKVGTVC----LTDQRPAP--GKRCYITGWG-AINGDTQQSPDILQQAMLPIASHENCRRKY---GDVSSTAHLCAGEARSDAAGGCNGDSGGPLVCEDNGSWFLHGAVSYGLRNCPTTHYTVFARVASYTDWI 232          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Nematostella
Match: EDO48368 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJF4])

HSP 1 Score: 101.679 bits (252), Expect = 3.190e-25
Identity = 73/286 (25.52%), Postives = 124/286 (43.36%), Query Frame = 1
Query:   97 IVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGL--CEGDSGSGLACKKGNKWTLAGVASATHAE-LPGNYPGIFTRVSYFISWI 945
            ++ G DA    + W ISL+     +   + CG SLI  ++++TAAHCF         N+ D  ++    G                        +    +G DG+            + + ++I H ++    +  DIAL++L  P+    L+E +  + LPS  D       G  C + GWG    GG  +   Q A + I S+K C    ++   +++E   CAG   +G  +  C+GDSG    C++  +W L G  S  +   L  N+  +F R+S +++WI
Sbjct:    1 MITGTDAVPHSWPWQISLETTKDRNRWFHTCGGSLISPEYIVTAAHCFP--------NNPDVTMFRVVVG------------------------QHDRLNGGDGQ----------TPIAIHEVIKHESFSMRHLRNDIALIRLVKPVT---LSERVGTVCLPSHGDRI---TPGTKCFITGWGRTVGGGQSARILQQAEMPIASHKDCSAANSRLVPVHEESMLCAGHATTGVHVSGCQGDSGGPFVCEESGRWVLRGAVSWGNPRCLAENFYTVFARMSSYVNWI 238          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000028221.1 (tryptase [Source:NCBI gene;Acc:101156896])

HSP 1 Score: 122.865 bits (307), Expect = 1.711e-32
Identity = 99/337 (29.38%), Postives = 141/337 (41.84%), Query Frame = 1
Query:   49 LFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN**KFNCLN*KKIVILQVDTYN--TILTRSE 1053
            +FVL+ +        RIV G+DA   ++ W   L+  IP       CG SLI+ QW+L+AAHCF                                             + TS+   + G+  ++ +    + L VSKII+HPNY       D+ L+KL SP+     N+++  + L +     P    G    + G+G  ++GG ++   Q  N+ I SN  C   Y  G   N     CAG    G   C+GDSG  L  K   +W  AGV S        N+PG++ RVS F SWI   ++        CLN K  +      YN  TI  RSE
Sbjct:    5 MFVLLQTL-------RIVGGQDAAAGQWPWQAMLQ--IPVAGGTALCGGSLINSQWILSAAHCF---------------------------------------------KSTSNVVVSLGRITEQGSNPHQVSLSVSKIIVHPNYDSRTNNNDLTLLKLASPVT---FNDYISPVCLAAAGSDFPG---GTSSWVTGFGTLSSGGPLASTLQEVNIPIVSNTQCNSAY--GGITN--QMICAGLTTGGLDSCQGDSGGPLVIKNSTRWVQAGVVSFGEGCAKPNFPGVYARVSEFQSWISSQVDGPT--NSYCLNIKTFI------YNETTICQRSE 269          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000029022.1 (tryptase [Source:NCBI gene;Acc:101156896])

HSP 1 Score: 122.094 bits (305), Expect = 2.574e-32
Identity = 87/291 (29.90%), Postives = 123/291 (42.27%), Query Frame = 1
Query:   82 VISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILEN 954
            V   RIV G+DA   ++ W   L+  IP       CG SLI+ QW+L+AAHCF                                             + TS+   + G+  ++ +    + L VSKII+HPNY       D+ L+KL SP+     N+++  + L +     P    G    + G+G  ++GG ++   Q  N+ I SN  C   Y  G   N     CAG    G   C+GDSG  L  K   +W  AGV S        N+PG++ RVS F SWI  N
Sbjct:   23 VTESRIVGGQDAAAGQWPWQAMLQ--IPVAGGTALCGGSLINSQWILSAAHCF---------------------------------------------KSTSNVVVSLGRITEQGSNPHQVSLSVSKIIVHPNYDSRTNNNDLTLLKLASPVT---FNDYISPVCLAAAGSDFPG---GTSSWVTGFGTLSSGGPLASTLQEVNIPIVSNTQCNSAY--GGITN--QMICAGLTTGGLDSCQGDSGGPLVIKNSTRWVQAGVVSFGEGCAKPNFPGVYARVSEFQSWIRRN 256          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000029624.1 (tryptase [Source:NCBI gene;Acc:101156896])

HSP 1 Score: 121.709 bits (304), Expect = 3.239e-32
Identity = 87/291 (29.90%), Postives = 123/291 (42.27%), Query Frame = 1
Query:   82 VISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILEN 954
            V   RIV G+DA   ++ W   L+  IP       CG SLI+ QW+L+AAHCF                                             + TS+   + G+  ++ +    + L VSKII+HPNY       D+ L+KL SP+     N+++  + L +     P    G    + G+G  ++GG ++   Q  N+ I SN  C   Y  G   N     CAG    G   C+GDSG  L  K   +W  AGV S        N+PG++ RVS F SWI  N
Sbjct:   16 VTESRIVGGQDAAAGQWPWQAMLQ--IPVAGGTALCGGSLINSQWILSAAHCF---------------------------------------------KSTSNVVVSLGRITEQGSNPHQVSLSVSKIIVHPNYDSRTNNNDLTLLKLASPVT---FNDYISPVCLAAAGSDFPG---GTSSWVTGFGTLSSGGPLASTLQEVNIPIVSNTQCNSAY--GGITN--QMICAGLTTGGLDSCQGDSGGPLVIKNSTRWVQAGVVSFGEGCAKPNFPGVYARVSEFQSWIRRN 249          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000000316.2 (tryptase [Source:NCBI gene;Acc:101156896])

HSP 1 Score: 120.553 bits (301), Expect = 8.774e-32
Identity = 88/304 (28.95%), Postives = 129/304 (42.43%), Query Frame = 1
Query:   49 LFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENIN 960
            +FVL+ +        RIV G+DA   ++ W   L+  IP       CG SLI+ QW+L+AAHCF                                             + TS+   + G+  ++ +    + L VSKII+HPNY       D+ L+KL SP+     N+++  + L +     P    G    + G+G  ++GG ++   Q  N+ I SN  C   Y  G   N     CAG    G   C+GDSG  L  K   +W  AGV S        N+PG++ RVS F SWI   ++
Sbjct:    5 MFVLLQTL-------RIVGGQDAAAGQWPWQAMLQ--IPVAGGTALCGGSLINSQWILSAAHCF---------------------------------------------KSTSNVVVSLGRITEQGSNPHQVSLSVSKIIVHPNYDSRTNNNDLTLLKLASPVT---FNDYISPVCLAAAGSDFPG---GTSSWVTGFGTLSSGGPLASTLQEVNIPIVSNTQCNSAY--GGITN--QMICAGLTTGGLDSCQGDSGGPLVIKNSTRWVQAGVVSFGEGCAKPNFPGVYARVSEFQSWISSQVD 244          
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000028466.1 (tryptase [Source:NCBI gene;Acc:101156896])

HSP 1 Score: 120.939 bits (302), Expect = 1.206e-31
Identity = 96/325 (29.54%), Postives = 135/325 (41.54%), Query Frame = 1
Query:   85 ISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN**KFNCLN*KKIVILQVDTYN--TILTRSE 1053
            I   IV G+DA   ++ W   L+  IP       CG SLI+ QW+L+AAHCF                                             + TS+   + G+  ++ +    + L VSKII+HPNY       D+ L+KL SP+     N+++  + L +     P    G    + G+G  ++GG ++   Q  N+ I SN  C   Y  G   N     CAG    G   C+GDSG  L  K   +W  AGV S        N+PG++ RVS F SWI   ++        CLN K  +      YN  TI  RSE
Sbjct:   16 IDGEIVGGQDAAAGQWPWQAMLQ--IPVAGGTALCGGSLINSQWILSAAHCF---------------------------------------------KSTSNVVVSLGRITEQGSNPHQVSLSVSKIIVHPNYDSRTNNNDLTLLKLASPVT---FNDYISPVCLAAAGSDFPG---GTSSWVTGFGTLSSGGPLASTLQEVNIPIVSNTQCNSAY--GGITN--QMICAGLTTGGLDSCQGDSGGPLVIKNSTRWVQAGVVSFGEGCAKPNFPGVYARVSEFQSWISSQVDGPT--NSYCLNIKTFI------YNETTICQRSE 275          
BLAST of Peptidase S1 domain-containing protein vs. Planmine SMEST
Match: SMESG000012752.1 (SMESG000012752.1)

HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0
Identity = 316/317 (99.68%), Postives = 316/317 (99.68%), Query Frame = 1
Query:   19 MKRLFILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN 969
            MKRLFILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRR PESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN
Sbjct:    1 MKRLFILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRVPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN 317          
BLAST of Peptidase S1 domain-containing protein vs. Planmine SMEST
Match: SMESG000012752.1 (SMESG000012752.1)

HSP 1 Score: 649.818 bits (1675), Expect = 0.000e+0
Identity = 316/319 (99.06%), Postives = 316/319 (99.06%), Query Frame = 1
Query:   19 MKRLFILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFD--GTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN 969
            MKRLFILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFD  GTDGKWDKRR PESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN
Sbjct:    1 MKRLFILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDDLGTDGKWDKRRVPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN 319          
BLAST of Peptidase S1 domain-containing protein vs. Planmine SMEST
Match: SMESG000012752.1 (SMESG000012752.1)

HSP 1 Score: 649.432 bits (1674), Expect = 0.000e+0
Identity = 316/320 (98.75%), Postives = 316/320 (98.75%), Query Frame = 1
Query:   19 MKRLFILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDG---TDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN 969
            MKRLFILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDG   TDGKWDKRR PESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN
Sbjct:    1 MKRLFILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKYEKAYKGWYEETSDFDGMKRTDGKWDKRRVPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQN 320          
BLAST of Peptidase S1 domain-containing protein vs. Planmine SMEST
Match: SMESG000077268.1 (SMESG000077268.1)

HSP 1 Score: 235.343 bits (599), Expect = 2.344e-75
Identity = 127/326 (38.96%), Postives = 177/326 (54.29%), Query Frame = 1
Query:   31 FILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQ----WLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKYEHKY----------EKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENINKQ 966
            +I   +  + + S S   I KR++ G+ AP DKY W++S+KGD+   +    CGAS+I  +    WLLTAAHCF  + +    + T P LWHAR G  K++  Y K+   Y           KA+K  +  + +   T              D+H+ KI +HP+Y    +E DIALMKL  P+  + L   +  I LP   +   WP +G  C+++GWGC    G  +  A++A LK+  N  C   +    DLN E EFCAG  K  +GLC GDSGSGLACK   +W LAGV SA H++ PG +P IFT+V  F +WIL+ INK 
Sbjct:    3 YIEFLSSLIFVVSISTGQIDKRVIGGETAPPDKYRWAVSIKGDVDELKTSGACGASIIKTKGNISWLLTAAHCFFPSNKIKTFDLTKPELWHARVGLMKVDFYYKKHGRGYLSNNKLISFFSKAFKKLFGNSKNVVTTG-------------DIHIKKIFIHPDYVSTRLENDIALMKLIDPLPIEQLKG-VNTIELPDIPNLTKWPPVGSDCVVVGWGCTKAAGVPNTYAKLALLKVWDNDKCIDAFVAPIDLNPESEFCAGYLKRKYGLCPGDSGSGLACKYDGRWMLAGVVSAAHSKEPGQFPAIFTKVPSFKNWILDTINKN 314          
BLAST of Peptidase S1 domain-containing protein vs. Planmine SMEST
Match: SMESG000040907.1 (SMESG000040907.1)

HSP 1 Score: 203.371 bits (516), Expect = 1.017e-62
Identity = 121/329 (36.78%), Postives = 177/329 (53.80%), Query Frame = 1
Query:   19 MKRLFILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISLKGDIPTDEAVYGCGASLIDKQ----WLLTAAHCFLAAQEKSLLNSTDPILWHARAGARKLETEYVKY--EHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDLHVSKIILHPNYKPD---------EIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSKPWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADLNDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGNYPGIFTRVSYFISWILENIN 960
            MK         F+   + + + + KRI+ G++AP DKY W++S+KG++   +    CGAS++       WL+TAAHCF  +   ++ N T    WHAR G  K++  Y K+  + K+++  K  + +   +    GK DK +      DLH+SKII+HP Y+P           ++ DIALMKL+ P+    L+  +  I LP+ + +  WP IG  CI++GWGC     + +  A++A LKI SN  CE        +N   +FCAG  K  +GLC GDSGSGL C      T+AGV S  H   PG YP +F+RVS F  WI   I+
Sbjct:    1 MKNTIYQFLIAFIFCVTCTHSSMEKRIIGGEEAPPDKYRWAVSIKGNVDKLKMSGACGASILQTPGQVLWLVTAAHCFYPSDGVTVYNLTKGKYWHARVGLTKVDFLYQKHGMKDKFKEKVKSLFNKA--YRKLFGK-DKFKVTTD--DLHISKIIIHPGYQPAVKSNDDGMFRMDNDIALMKLKEPLPIHQLSG-VSTIGLPNWSVNTSWPAIGSDCIVVGWGCTKVKNAPNPIAKLAILKIWSNSECENSVPSKLYINPASDFCAGYLKKKYGLCHGDSGSGLICDYQGTPTIAGVVSRGHGSDPGGYPAVFSRVSAFKEWIFRVIS 323          
The following BLAST results are available for this feature:
BLAST of Peptidase S1 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TPSAB12.338e-2828.52tryptase alpha/beta 1 [Source:HGNC Symbol;Acc:HGNC... [more]
KLKB12.370e-2831.38kallikrein B1 [Source:HGNC Symbol;Acc:HGNC:6371][more]
TPSAB12.733e-2828.52tryptase alpha/beta 1 [Source:HGNC Symbol;Acc:HGNC... [more]
TPSB23.118e-2828.19tryptase beta 2 (gene/pseudogene) [Source:HGNC Sym... [more]
TPSB23.605e-2828.19tryptase beta 2 (gene/pseudogene) [Source:HGNC Sym... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
svh-13.062e-2028.77HGF/MSP/plasminogen-like protein [Source:UniProtK... [more]
try-11.846e-1622.41TRYpsin-like protease [Source:UniProtKB/TrEMBL;Ac... [more]
try-37.222e-1423.88TRYpsin-like protease [Source:UniProtKB/TrEMBL;Ac... [more]
try-32.422e-1124.36TRYpsin-like protease [Source:UniProtKB/TrEMBL;Ac... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG187351.799e-2227.84gene:FBgn0042098 transcript:FBtr0071744[more]
CG33552.471e-2228.37gene:FBgn0031619 transcript:FBtr0077376[more]
Sb8.942e-2229.01gene:FBgn0003319 transcript:FBtr0344008[more]
Sb8.942e-2229.01gene:FBgn0003319 transcript:FBtr0083250[more]
mas1.777e-2127.46gene:FBgn0011653 transcript:FBtr0332659[more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CR352265.12.513e-3227.89pep chromosome:GRCz11:24:21813127:21819010:-1 gene... [more]
tmprss3b1.073e-2831.89transmembrane serine protease 3b [Source:ZFIN;Acc:... [more]
tmprss3a1.562e-2730.56transmembrane serine protease 3a [Source:ZFIN;Acc:... [more]
tmprss3a1.626e-2730.56transmembrane serine protease 3a [Source:ZFIN;Acc:... [more]
zgc:1008681.511e-2629.59zgc:100868 [Source:ZFIN;Acc:ZDB-GENE-040801-33][more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TMPRSS93.617e-2929.01transmembrane serine protease 9 [Source:NCBI gene;... [more]
TMPRSS95.266e-2929.01transmembrane serine protease 9 [Source:NCBI gene;... [more]
pcdh8l2.582e-2627.99protocadherin-8-like [Source:Xenbase;Acc:XB-GENE-1... [more]
F112.051e-2529.31kallikrein B1 [Source:NCBI gene;Acc:100486524][more]
ENSXETT00000016164.14.021e-2529.10pep primary_assembly:Xenopus_tropicalis_v9.1:9:254... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Klkb18.483e-3029.83kallikrein B, plasma 1 [Source:MGI Symbol;Acc:MGI:... [more]
Tpsab11.817e-2827.95tryptase alpha/beta 1 [Source:MGI Symbol;Acc:MGI:9... [more]
Tmprss92.998e-2728.18transmembrane protease, serine 9 [Source:MGI Symbo... [more]
Tmprss92.998e-2728.18transmembrane protease, serine 9 [Source:MGI Symbo... [more]
Prss284.188e-2730.10protease, serine 28 [Source:MGI Symbol;Acc:MGI:214... [more]
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BLAST of Peptidase S1 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q2KJ63|KLKB1_BOVIN2.079e-2930.51Plasma kallikrein OS=Bos taurus OX=9913 GN=KLKB1 P... [more]
sp|P26262|KLKB1_MOUSE5.934e-2929.83Plasma kallikrein OS=Mus musculus OX=10090 GN=Klkb... [more]
sp|Q9N2D1|TRYT_PIG2.799e-2829.33Tryptase OS=Sus scrofa OX=9823 GN=MCT7 PE=2 SV=1[more]
sp|Q02844|TRYB1_MOUSE5.927e-2827.95Tryptase OS=Mus musculus OX=10090 GN=Tpsab1 PE=2 S... [more]
sp|P14272|KLKB1_RAT8.556e-2829.79Plasma kallikrein OS=Rattus norvegicus OX=10116 GN... [more]
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BLAST of Peptidase S1 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A074Z6751.632e-6739.63Peptidase S1 domain-containing protein OS=Opisthor... [more]
A0A4S2MLJ22.791e-6538.89Peptidase S1 domain-containing protein OS=Opisthor... [more]
A0A4E0RH516.772e-6539.86Subfamily S1A unassigned peptidase (S01 family) OS... [more]
A0A5J4P1021.119e-6435.34Peptidase S1 domain-containing protein OS=Paragoni... [more]
G7Y3P92.595e-6439.20Serine protease 33 OS=Clonorchis sinensis OX=79923... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000033802.16.346e-3028.91pep primary_assembly:Astyanax_mexicanus-2.0:3:2189... [more]
ENSAMXT00000014374.21.718e-2727.68chymotrypsinogen A-like [Source:NCBI gene;Acc:1030... [more]
ENSAMXT00000014359.22.824e-2727.68pep primary_assembly:Astyanax_mexicanus-2.0:8:2090... [more]
ctrb16.914e-2627.10chymotrypsin A-like [Source:NCBI gene;Acc:10302748... [more]
ENSAMXT00000049166.11.216e-2528.33serine protease 56 [Source:NCBI gene;Acc:103021696... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000002258.11.056e-2427.55pep scaffold:Pmarinus_7.0:GL487751:502724:507790:1... [more]
ENSPMAT00000002946.11.425e-2426.55pep scaffold:Pmarinus_7.0:GL477988:107792:112981:-... [more]
ENSPMAT00000005493.12.198e-2426.21pep scaffold:Pmarinus_7.0:GL476632:243920:250733:-... [more]
tmprss3a2.792e-2125.35transmembrane serine protease 3a [Source:ZFIN;Acc:... [more]
ENSPMAT00000001315.17.891e-2127.93pep scaffold:Pmarinus_7.0:GL479305:249343:254401:-... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO334595.627e-3229.93Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO396259.271e-2729.34Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO410356.357e-2626.92Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO442088.499e-2628.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO483683.190e-2525.52Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Peptidase S1 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000028221.11.711e-3229.38tryptase [Source:NCBI gene;Acc:101156896][more]
ENSORLT00000029022.12.574e-3229.90tryptase [Source:NCBI gene;Acc:101156896][more]
ENSORLT00000029624.13.239e-3229.90tryptase [Source:NCBI gene;Acc:101156896][more]
ENSORLT00000000316.28.774e-3228.95tryptase [Source:NCBI gene;Acc:101156896][more]
ENSORLT00000028466.11.206e-3129.54tryptase [Source:NCBI gene;Acc:101156896][more]
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BLAST of Peptidase S1 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000012752.10.000e+099.68SMESG000012752.1[more]
SMESG000012752.10.000e+099.06SMESG000012752.1[more]
SMESG000012752.10.000e+098.75SMESG000012752.1[more]
SMESG000077268.12.344e-7538.96SMESG000077268.1[more]
SMESG000040907.11.017e-6236.78SMESG000040907.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30034156 ID=SMED30034156|Name=Peptidase S1 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1055bp
ATTAAGTTCCATTTCGAGATGAAAAGATTATTTATTCTACTTTGCAATTT
ATTCGTTTTAATTTATTCTCGATCATTTAACGTAATCTCAAAAAGAATTG
TTAATGGCAAAGACGCTCCTGAGGACAAATATTTATGGTCTATATCATTG
AAAGGTGATATTCCGACTGATGAAGCTGTTTATGGCTGCGGAGCTTCGCT
GATTGATAAACAGTGGCTTTTGACGGCTGCGCATTGCTTCCTTGCTGCCC
AAGAAAAAAGTTTATTGAACTCAACAGATCCAATTTTATGGCACGCAAGA
GCAGGAGCTAGAAAATTAGAGACAGAATATGTAAAATATGAGCATAAATA
TGAAAAGGCTTATAAAGGCTGGTATGAAGAAACCAGTGATTTCGATGGAA
CTGATGGAAAATGGGATAAAAGAAGAGCTCCAGAGAGTTATCTCGATTTA
CATGTATCCAAAATAATTTTGCATCCAAATTATAAGCCAGATGAAATTGA
ATGGGATATAGCATTAATGAAGCTTCAATCTCCCATTGAAGAAAAATATC
TCAATGAATTCCTTGAAGTAATTCCTCTGCCGAGCGAAGCTGATTCGAAG
CCATGGCCGAAAATCGGTCAAATGTGCATTTTAATTGGGTGGGGTTGCAA
AACCAATGGCGGGTCTATTTCAGAAAGAGCACAAATTGCTAATTTAAAGA
TACAATCAAATAAATTTTGTGAAAAGGTTTATAATCAGGGGGCAGATTTA
AATGATGAGCATGAGTTTTGTGCGGGAAAATACAAAAGTGGACACGGTTT
ATGTGAAGGGGACAGTGGAAGTGGCTTGGCATGTAAGAAAGGAAACAAAT
GGACGTTAGCTGGCGTGGCTTCTGCTACCCATGCTGAATTGCCGGGAAAT
TATCCAGGTATTTTCACCCGAGTATCCTATTTTATCTCATGGATTCTGGA
AAATATTAATAAGCAAAATTAGTAAAAATTTAATTGTTTAAATTAGAAAA
AAATAGTGATTCTACAAGTAGATACGTATAATACAATATTAACGAGATCG
GAAGA
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protein sequence of SMED30034156-orf-1

>SMED30034156-orf-1 ID=SMED30034156-orf-1|Name=SMED30034156-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=324bp
IKFHFEMKRLFILLCNLFVLIYSRSFNVISKRIVNGKDAPEDKYLWSISL
KGDIPTDEAVYGCGASLIDKQWLLTAAHCFLAAQEKSLLNSTDPILWHAR
AGARKLETEYVKYEHKYEKAYKGWYEETSDFDGTDGKWDKRRAPESYLDL
HVSKIILHPNYKPDEIEWDIALMKLQSPIEEKYLNEFLEVIPLPSEADSK
PWPKIGQMCILIGWGCKTNGGSISERAQIANLKIQSNKFCEKVYNQGADL
NDEHEFCAGKYKSGHGLCEGDSGSGLACKKGNKWTLAGVASATHAELPGN
YPGIFTRVSYFISWILENINKQN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR001314Peptidase_S1A
IPR001254Trypsin_dom
IPR018114TRYPSIN_HIS
IPR009003Peptidase_S1_PA
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
Vocabulary: molecular function
TermDefinition
GO:0004252serine-type endopeptidase activity
GO:0008233peptidase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001314Peptidase S1A, chymotrypsin familyPRINTSPR00722CHYMOTRYPSINcoord: 265..277
score: 44.56
coord: 165..179
score: 36.18
coord: 64..79
score: 56.58
IPR001254Serine proteases, trypsin domainSMARTSM00020trypsin_2coord: 32..315
e-value: 5.8E-43
score: 158.7
IPR001254Serine proteases, trypsin domainPFAMPF00089Trypsincoord: 33..119
e-value: 1.0E-8
score: 35.3
coord: 142..315
e-value: 4.2E-32
score: 111.8
IPR001254Serine proteases, trypsin domainPROSITEPS50240TRYPSIN_DOMcoord: 33..320
score: 26.418
IPR001254Serine proteases, trypsin domainCDDcd00190Tryp_SPccoord: 33..318
e-value: 1.24377E-58
score: 186.328
NoneNo IPR availableGENE3DG3DSA:2.40.10.10coord: 26..119
e-value: 1.5E-13
score: 52.6
coord: 120..321
e-value: 8.4E-43
score: 148.5
NoneNo IPR availablePANTHERPTHR24256TRANSMEMBRANE PROTEASE, SERINEcoord: 29..321
IPR018114Serine proteases, trypsin family, histidine active sitePROSITEPS00134TRYPSIN_HIScoord: 74..79
IPR009003Peptidase S1, PA clanSUPERFAMILYSSF50494Trypsin-like serine proteasescoord: 18..321