SMED30034099
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Alignments
SMED30034099 aligns in the following genomic locations:
Homology
BLAST of SMED30034099 vs. Ensembl Fly
Match: Usp8 (gene:FBgn0038862 transcript:FBtr0304714) HSP 1 Score: 49.2914 bits (116), Expect = 9.383e-6 Identity = 49/246 (19.92%), Postives = 101/246 (41.06%), Query Frame = 2
Query: 332 IDEQDNSDVLSFLINRMHHDIKT----------------KIKIQKSCSNNLLNIKFPIIMTVKYDIICQCEKVIFSTVQSQISLNLTVSSKYTNLQNLFDKIFLGEVVKGFNCETRNCSHKK-AVRVCKLCKPLPETICIRLQGRNSFITLEILNEMLIKRSFVIDVSRDDFEEPPNQQTNSNRMENDYRYFLKAI-----VQNNTNFRYQCYKNDRNKWFLISDESLVECDQPKILEDKDYILFF 1003
+D+QD+ + L+ L++ +H D++T ++ K+ + +L++ + + + + C E + + S +SL L +S L D F GE + G+NC + C K+ A++ + K LP + + L+ F + +K+ + ++ + P +R Y L A+ ++ C + KWF D+ + D ++ YILF+
Sbjct: 119 VDQQDSHEFLTILMDWLHSDLQTLHVPRQREMISASEKAWLEFTKAKESMILHLFYGQMKSTVKCVACHKESATYESF-SNLSLELPPNSNVCQLNQCMDMYFSGERIHGWNCPS--CKTKRDAIKKLDISK-LPPVLVVHLK---RFYADPSNSGSYMKKQNYLRFPLENLDMNPYIARAESRAVTPKTYQLYAVSNHYGTMEGGHYTAFCKSANYGKWFKFDDQVVSALDSSNVVSSAAYILFY 357
BLAST of SMED30034099 vs. Planmine SMEST
Match: SMESG000040016.1 (SMESG000040016.1) HSP 1 Score: 1340.87 bits (3469), Expect = 0.000e+0 Identity = 679/679 (100.00%), Postives = 679/679 (100.00%), Query Frame = 2
Query: 86 MGQLQSFFNLLAKDESLYSFDSTMNQSKSSCSDIVSKLTKLQSEYPNLEELNMLIELVEILQRDSEMKWKKSQNSIFKPRSKIDEQDNSDVLSFLINRMHHDIKTKIKIQKSCSNNLLNIKFPIIMTVKYDIICQCEKVIFSTVQSQISLNLTVSSKYTNLQNLFDKIFLGEVVKGFNCETRNCSHKKAVRVCKLCKPLPETICIRLQGRNSFITLEILNEMLIKRSFVIDVSRDDFEEPPNQQTNSNRMENDYRYFLKAIVQNNTNFRYQCYKNDRNKWFLISDESLVECDQPKILEDKDYILFFENQISVPSISGSRVTSLYASKDNFPSSLVTPLSSHRKLYSRFKSKDIMDSSQKSIKELSNFELNYQQSISRKSPVSLKKLGINQYLGICLHSLSSCEVLIKRIQRITQKSMRKMKTFINLIMISDGDYRNAEISNSIIKEWNALYKTNLFHLLLALMNDLRNEIRIQNMNPYPLEINMTVRISCVNCGKHFQYQLTESAFTIAVSGSISNLSLQDSIDMIFQEQILNCYXXXXXXXXXXXXXXXXIRMRQPIASTLCVHVEKLIWNGRQNQFIFSKINFTDVLIMKLNYYENNSDRNSKLLSSCNEIFLETAVKKELVYHLQSLISCQFNDKYMCYKKLGNDWYCLTDTAMSNVSLNQVLESDPYILFYELLT 2122
MGQLQSFFNLLAKDESLYSFDSTMNQSKSSCSDIVSKLTKLQSEYPNLEELNMLIELVEILQRDSEMKWKKSQNSIFKPRSKIDEQDNSDVLSFLINRMHHDIKTKIKIQKSCSNNLLNIKFPIIMTVKYDIICQCEKVIFSTVQSQISLNLTVSSKYTNLQNLFDKIFLGEVVKGFNCETRNCSHKKAVRVCKLCKPLPETICIRLQGRNSFITLEILNEMLIKRSFVIDVSRDDFEEPPNQQTNSNRMENDYRYFLKAIVQNNTNFRYQCYKNDRNKWFLISDESLVECDQPKILEDKDYILFFENQISVPSISGSRVTSLYASKDNFPSSLVTPLSSHRKLYSRFKSKDIMDSSQKSIKELSNFELNYQQSISRKSPVSLKKLGINQYLGICLHSLSSCEVLIKRIQRITQKSMRKMKTFINLIMISDGDYRNAEISNSIIKEWNALYKTNLFHLLLALMNDLRNEIRIQNMNPYPLEINMTVRISCVNCGKHFQYQLTESAFTIAVSGSISNLSLQDSIDMIFQEQILNCYNCENKKCSNKTKSKKSIRMRQPIASTLCVHVEKLIWNGRQNQFIFSKINFTDVLIMKLNYYENNSDRNSKLLSSCNEIFLETAVKKELVYHLQSLISCQFNDKYMCYKKLGNDWYCLTDTAMSNVSLNQVLESDPYILFYELLT
Sbjct: 1 MGQLQSFFNLLAKDESLYSFDSTMNQSKSSCSDIVSKLTKLQSEYPNLEELNMLIELVEILQRDSEMKWKKSQNSIFKPRSKIDEQDNSDVLSFLINRMHHDIKTKIKIQKSCSNNLLNIKFPIIMTVKYDIICQCEKVIFSTVQSQISLNLTVSSKYTNLQNLFDKIFLGEVVKGFNCETRNCSHKKAVRVCKLCKPLPETICIRLQGRNSFITLEILNEMLIKRSFVIDVSRDDFEEPPNQQTNSNRMENDYRYFLKAIVQNNTNFRYQCYKNDRNKWFLISDESLVECDQPKILEDKDYILFFENQISVPSISGSRVTSLYASKDNFPSSLVTPLSSHRKLYSRFKSKDIMDSSQKSIKELSNFELNYQQSISRKSPVSLKKLGINQYLGICLHSLSSCEVLIKRIQRITQKSMRKMKTFINLIMISDGDYRNAEISNSIIKEWNALYKTNLFHLLLALMNDLRNEIRIQNMNPYPLEINMTVRISCVNCGKHFQYQLTESAFTIAVSGSISNLSLQDSIDMIFQEQILNCYNCENKKCSNKTKSKKSIRMRQPIASTLCVHVEKLIWNGRQNQFIFSKINFTDVLIMKLNYYENNSDRNSKLLSSCNEIFLETAVKKELVYHLQSLISCQFNDKYMCYKKLGNDWYCLTDTAMSNVSLNQVLESDPYILFYELLT 679
BLAST of SMED30034099 vs. Planmine SMEST
Match: SMESG000039991.1 (SMESG000039991.1) HSP 1 Score: 166.007 bits (419), Expect = 2.169e-42 Identity = 155/562 (27.58%), Postives = 265/562 (47.15%), Query Frame = 2
Query: 443 IKFPIIMTVKYDIICQCEKVIFSTVQSQISLNLTVSSKYTNLQNLFDKIFLGEVVKGFNCETRNCSHKKAVRVCKLCKPLPETICIRLQGRNSFITLEILNEMLIKRSFVIDVSRDDFEEPPNQQTNSNRMENDYRYFLKAIVQNNTNFRYQCYKNDRNKWFLISDESLVECDQPKILEDKDYILFFENQISVPSISGSRVTSLYASKDNFPSSLVTPLSSHRKLYSRFKSKDIMDSSQKSIKELSNFELNYQQSISRKSPVSLKKLGINQYLGICLHSLSSCEVLIKRIQRITQKSMRKMKTFINLIMISDGDYRNAEISNSIIKEWNALYKTNLFHLLLALMNDLRNEIRIQNMNPYPLEINMTVRISCVNCGKHFQYQLT-ESAFTIAVSGSISNLSLQDSIDMIFQEQILNCYXXXXXXXXXXXXXXXXIRMRQPIASTLCVHVEKLIWNGRQNQFIFSKINFTDVLIMKLNYYENNSDRNSKLLSSCNEIFLETAVKKELVYHLQSLIS---CQFNDKYMCYKKLGNDWYCLTDTAMSNVSLNQVLESDPYILFYEL 2116
+K PI ++V +I C+C + +S+ L L V + +LQ D IF E +NC L +P+P+ IC+ + + +++L E++I+ S+ S+ D + D +YFL +I+ + N Y+CY+ W+ I D+ E D+ L K +IL +EN + + T Y K F S+++P RK+ + D+ K++ FE Y IS+ S S K L ICLH+ SSC+ + IQ I + M K F+ I + + + EI + E + L +TN+F L L+++L E + MNP+ + +T+R C C + L +F I + +I + +QD ++ F+ +IL ++C K C K S R+ P + +C+HV ++IW+G +N + S I ++ L +KL Y + S N K+L+Y LQS+IS ++Y+C+K + +W+ +T ++ VS N+++ PYI+FYEL
Sbjct: 469 VKCPINISVMSEIKCKCNETTLKVNKSK--LGLKVFNFCISLQRSIDFIFNVEKEVTLKDNNKNCYCTSGFSKSNLSQPIPKIICVYSENESRLFQIKLLKELVIRNSY-FSQSQLDVRSHSENGKQRTLEKTDTKYFLTSIIGSLDN-GYECYRMCDGSWYHIKDDKSSEIDESANLFGKWHILLYEN------LKETHQTK-YNLKKTFKPSIISP----RKIC-------VEDAIMKNV-----FEFEY---ISKSSNSSHKAYC----LQICLHAFSSCKKFVNEIQNILTEEMEKSNLFMKYITLLLQSFADVEIPLEAVNELHLLEETNIFQFLKDLLSNLSTEWK---MNPFLISRRVTIR--CCLCETLQEDDLNCNLSFNIPATSNIGFIDIQDIVNSSFKIEILKNFDCIKKDCGGKESKMLS-RLCLPFPTVICIHVVRVIWDGLKNVLVNSNIRISEQLQIKLKYLDYQSSINYS--------------NKDLIYILQSVISFTESPVPNEYICFKNVNENWFKITKSSTLRVSKNEMMTYQPYIMFYEL 976 HSP 2 Score: 96.2857 bits (238), Expect = 3.755e-20 Identity = 166/687 (24.16%), Postives = 301/687 (43.81%), Query Frame = 2
Query: 161 QSKSSCSDIVSKLTKLQSEYPNLEEL----NMLIELVEILQRDSEMKWKKSQNSIFKPRSKIDEQDNSDVLSFLINRMHHDIKTKIKIQKSCSNNLLNIKFPIIMTVKYDIICQCEKVIFSTVQSQISLNLTVSSK----YTNLQNLFDKIFLGEVVKGFNCETRNCSHKKAVRVCKLCKPLPETICIRLQ------GRNSFIT--LEILNEMLIKRSFVIDVSRDDFEEPPNQQTNSNRMENDYRYFLKAIVQNNTNFRYQ---CYKNDRNKWFLISDESLVECDQPKILEDKDYILFFENQISVP--SISGSRVTSLYASKDNFPSSLVTPLSSHRKLYSRFKSKDIMDSSQKSIKELSNFELNYQQSISRKSPVSLK--KLGINQYLGICLHSLSSCEVLIKRI-----QRITQKSMRKMKTFINLIMISDGDYRNAEISNSIIKEWNALYKTNLFHLLLALMNDLRNEIRIQNMNPYPLEINMTVRISCVNCGKHFQYQLTESAFTIAVSGSISNLSLQDSIDMIF---QEQIL-----NCYXXXXXXXXXXXXXXXXIRMRQPIASTLCVHVEKLIWNGRQNQFIFSKINFTDVLIMKLNYYENNSDRNSKLLSSCNEIFLETAVKKELVYHLQSLISCQFNDKYMCYKKLGNDWYCLTDTAMSNVSLNQVLESDPYILFYE 2113
Q S+CS++ + L KL+ +YP+L +L +L + +I R+ M + + Q IF+ ++ +QD+ D+L+ +IN ++ +++ + + + ++K + M + +I C E T Q ++L++ + K LQ+L + F E + CE ++CS K+ KLC +P T+CIRLQ G I +E E+ I S++ N Q N + + N+ +Y LK+++ N N ++ CY+ N WF ++D S+ + D IL Y+LF+EN S P ++ R T + N S P + L R K+K I +S+ +++ +K+P+ + + + Q+ + + +LS+C ++ + + I ++ + +K +N + N E+ I+ E +K L + A N +R +N P+ I++ I C C + ++ +S + V +SLQ SID IF +E L NCY S + QPI +CV+ E N+ +I L+++ +Y+ S + S T K + Y L S+I ++ Y CY+ WY + D S + + L +IL YE
Sbjct: 37 QGISACSNLAATLRKLELKYPSLAKLRTFNKILKDWCDIYVRN--MSFDEDQ--IFQHWNRPGQQDSYDLLAHVINDVYSELQNETNLFQG------SLKLNLPMQISLEITCCPECNNEKTFQFDLALHVPIIKKPRLKSIVLQDLLESYFNEETINE-KCE-KSCSKKQK----KLC-IVPSTLCIRLQRLNWINGELQIIRDRVEFTEELTISASYLT-----------NNQVNMS-LANNQKYHLKSVIAQNPNDSFEHNICYRLMNNNWFCVTDSSVHKEDFNNILNCIAYVLFYENS-SRPLRNLINLRSTFRRSETLNLSRSTRNPFIATIPLNMR-KTKTISLTSRIFRSSSADY---------KKTPLKRETCRFEMFQWEMVLIEALSTCRSFVEALKLINCENIGERYQQIVKCILNRSLSPIKIIDNKEV--DILGE----FKKFLAAIFPAEFNSSITNLRGRNKVKCPINISVMSEIKC-KCNET-TLKVNKSKLGLKVFNFC--ISLQRSIDFIFNVEKEVTLKDNNKNCYCTSGFSKS---------NLSQPIPKIICVYSE--------NESRLFQIKLLKELVIRNSYF---SQSQLDVRSHSENGKQRTLEKTDTKYFLTSIIG-SLDNGYECYRMCDGSWYHIKDDKSSEIDESANLFGKWHILLYE 652 HSP 3 Score: 93.9745 bits (232), Expect = 1.951e-19 Identity = 84/326 (25.77%), Postives = 155/326 (47.55%), Query Frame = 2
Query: 1187 ELNYQQSISRKSPVSLKKLGINQYLGICLHSLSSCEVLIKRIQRITQK--SMRKMKTFINLIM-ISDGDYRNAEI-SNSIIKEWNALYKTNLFHLLLALMNDLRNEIRIQ-NMNPYPLEINMTVRIS-----CVNCG--KHFQYQLTESAFTIAVSGSISNLSLQDSIDMIFQEQILN--CYXXXXXXXXXXXXXXXXIRMRQPIASTLCVHVEKLIWNGRQNQFIFSKINFTDVLIMKLNYYENNSDRNSKLLSSCNEIFLETAVKKELVYHLQSLISCQFNDKY---MCYKKLGNDWYCLTDTAMSNVSLNQVLESDPYILFYE 2113
E++ Q P+S N+YL I L +S+C L ++++ K S+ K++TF ++ D RN + I + WN + + + LL ++ND+ +E++ + N+ L++N+ ++IS C C K FQ+ L I + ++ LQD ++ F E+ +N C +KK + STLC+ +++L W + Q I ++ FT+ L + +Y NN + ++ YHL+S+I+ ND + +CY+ + N+W+C+TD+++ N +L Y+LFYE
Sbjct: 8 EIDLQDVFPAIKPLSSIMHRANRYLNIGLQGISACSNLAATLRKLELKYPSLAKLRTFNKILKDWCDIYVRNMSFDEDQIFQHWNRPGQQDSYDLLAHVINDVYSELQNETNLFQGSLKLNLPMQISLEITCCPECNNEKTFQFDLALHV-PIIKKPRLKSIVLQDLLESYFNEETINEKCEKSCSKKQKKLCI----------VPSTLCIRLQRLNWINGELQIIRDRVEFTEELTISASYLTNNQ--------------VNMSLANNQKYHLKSVIAQNPNDSFEHNICYRLMNNNWFCVTDSSVHKEDFNNILNCIAYVLFYE 308
BLAST of SMED30034099 vs. Planmine SMEST
Match: SMESG000039986.1 (SMESG000039986.1) HSP 1 Score: 112.464 bits (280), Expect = 3.490e-25 Identity = 86/298 (28.86%), Postives = 150/298 (50.34%), Query Frame = 2
Query: 161 QSKSSCSDIVSKLTKLQSEYPNLEELNMLIELVEILQRDSEMKWKKSQNSIFKPRSKIDEQDNSDVLSFLINRMHHDIKTKIKIQKSCSNNLLNIKFPIIMTVKYDIICQCEKVIFSTVQSQISLNLTVSSKYTN----LQNLFDKIFLGEVVKGFNCETRNCSHKKAVRVCKLCKPLPETICIRLQGRNSFITLEIL---------NEMLIKRSFVIDVSRDDFEEPPNQQTNSNRMENDYRYFLKAIVQN--NTNF-RYQCYKNDRNKWFLISDESLVECDQPKILEDKDYILFFE 1006
Q+ S+CS+ V L Q + PN +L L L++IL ++ S++ IF +K +QD+ ++L+F++N+++ + K ++ ++ L ++ P+ + +++ I C + + +QS+I+L++T+ + LQ+L D F E+++ F CE C A + KLC PLP TICI LQ R + I+ E +I R S + F E + N+Y Y LK+++ + N +F Y CYK N WF SD + + D + + YILF+E
Sbjct: 92 QAISACSNFVKDLNDCQMKNPNFPKLKTLNNLIKILCYGNKKYVDLSRDEIFAGWNKTGQQDSFELLAFIVNQVYKEFKKLNEV-----DDWLKVQLPMRIILEHHITCCSKYGGHNVMQSEIALHITIRKNQRSDPIILQDLIDFYFKKELIEDFICEESKCPGTSAKKQIKLCLPLPNTICIHLQ-RLHYTADNIMKKVEDKVKFEEKIIIRKAFCKRSDNSFSE----------LLNEYDYHLKSVIAHYGNASFGHYICYKKFNNSWFYTSDSFIYKEDIDHVFNCQAYILFYE 373 HSP 2 Score: 98.2117 bits (243), Expect = 8.255e-21 Identity = 80/291 (27.49%), Postives = 155/291 (53.26%), Query Frame = 2
Query: 1259 LGICLHSLSSCEVLIKRIQRITQKSMRKMKTFINLIMISDGDYRNAEISNSIIKEWNALYKTNLFHLLLALMNDLRNEIRIQNMNPYPLEINMTVRISCVNCGKHFQYQLTES--AFTIAVSGSISNLSLQDSIDMIFQEQILNCYXXXXXXXXXXXXXXXXIRMRQPIASTLCVHVEKLIWNGRQNQFIFSKINFTDVLIMKLNYYENNSDRNSKLLSSCNEIFLETAVKKELVYHLQSLISC-QFND--KYMCYKKLGNDWYCLTDTAMSNVSLNQVLESDPYILFYEL 2116
L +C H+LSSC+ +I + + + KM+ I+L+ + ++++ +++ + ++TN++HLL L+++L E N NP+ + + T I C +CG Q + ES +F I ++ S ++++QD +D FQ Q LN Y + SK ++ +P + +C+HVE+++W+G +N+ +I + + +K Y+ SD + K+L Y LQS+IS +++D +Y CY L +W +T + ++V+ +++ +PYI+ YEL
Sbjct: 946 LKMCFHALSSCKSVIN--NKYLKCADNKMEKSISLLKVLKSLDNQSDLAIDVMETFPESFETNMYHLLTLLLSNLSKE---WNHNPFLISRDKT--IECYHCGI-LQSRFLESQISFNIPITNSWDHVNIQDFVDKDFQIQTLN-YFECPRGECMGKTSKMLSKLCKPFPTVICIHVERVVWDGEKNKVFNLRIRLPEYIQLKKKYFNIQSDV--------------SNTDKDLNYTLQSVISFREYSDPIEYFCYNNLFGNWSKITKFSKTSVNKYEIMSCNPYIMLYEL 1213 HSP 3 Score: 90.1225 bits (222), Expect = 3.377e-18 Identity = 83/319 (26.02%), Postives = 160/319 (50.16%), Query Frame = 2
Query: 1211 SRKSPVSLKKLGINQYLGICLHSLSSCEVLIKRIQ--RITQKSMRKMKTFINLIMI-SDGDYRNAEIS-NSIIKEWNALYKTNLFHLLLALMNDLRNEIR-IQNMNPY-----PLEINMTVRISCVNCGKHFQYQLTESAFTIAVSGSISNLS----LQDSIDMIFQEQILNCYXXXXXXXXXXXXXXXXIRMRQPIASTLCVHVEKLIWNGRQ-NQFIFSKINFTDVLIMKLNYYENNSDRNSKLLSSCNEIFLETAVKKELVYHLQSLISCQFN---DKYMCYKKLGNDWYCLTDTAMSNVSLNQVLESDPYILFYE 2113
S + PVSL G N YL L ++S+C +K + ++ + K+KT NLI I G+ + ++S + I WN + + F LL ++N + E + + ++ + P+ I + I+C C K+ + + +S + ++ + S LQD ID F+++++ + CE KC + K+ I++ P+ +T+C+H+++L + + + K+ F + +I++ + + + + S+LL+ E YHL+S+I+ N Y+CYKK N W+ +D+ + ++ V YILFYE
Sbjct: 71 SERIPVSLINYGANCYLNSGLQAISACSNFVKDLNDCQMKNPNFPKLKTLNNLIKILCYGNKKYVDLSRDEIFAGWNKTGQQDSFELLAFIVNQVYKEFKKLNEVDDWLKVQLPMRIILEHHITC--CSKYGGHNVMQSEIALHITIRKNQRSDPIILQDLIDFYFKKELIEDFICEESKCPGTSAKKQ-IKLCLPLPNTICIHLQRLHYTADNIMKKVEDKVKFEEKIIIRKAFCKRSDNSFSELLN-------------EYDYHLKSVIAHYGNASFGHYICYKKFNNSWFYTSDSFIYKEDIDHVFNCQAYILFYE 373 HSP 4 Score: 55.0694 bits (131), Expect = 1.990e-7 Identity = 49/199 (24.62%), Postives = 90/199 (45.23%), Query Frame = 2
Query: 452 PIIMTVKYDIIC-QCEKVIFSTVQSQISLNLTVSSKY--TNLQNLFDKIFLGEVVKGFNCETRNCSHKKAVRVCKLCKPLPETICIRLQ------GRNSFITLEI-LNEML-IKRSFVIDVSRDDFEEPPNQQTNSNRMENDYRYFLKAIV---QNNTNFRYQCYKNDRNKWFLISDESLVECDQPKILEDKDYILFFE 1006
P +++ I C C + ++SQIS N+ +++ + N+Q+ DK F + + F C C K + + KLCKP P ICI ++ +N L I L E + +K+ + N Q++ + + D Y L++++ + + Y CY N W I+ S ++ +I+ YI+ +E
Sbjct: 1026 PFLISRDKTIECYHCGILQSRFLESQISFNIPITNSWDHVNIQDFVDKDFQIQTLNYFECPRGECMGKTSKMLSKLCKPFPTVICIHVERVVWDGEKNKVFNLRIRLPEYIQLKKKYF------------NIQSDVSNTDKDLNYTLQSVISFREYSDPIEYFCYNNLFGNWSKITKFSKTSVNKYEIMSCNPYIMLYE 1212
BLAST of SMED30034099 vs. Planmine SMEST
Match: SMESG000040010.1 (SMESG000040010.1) HSP 1 Score: 108.997 bits (271), Expect = 4.158e-25 Identity = 110/364 (30.22%), Postives = 176/364 (48.35%), Query Frame = 2
Query: 1220 SPVSLKKLGINQYLGICLHSLSSCEVLIKRIQRITQK--SMRKMKTFINLIMISDGDYRNA-EIS-NSIIKEWNALYKTNLFHLLLALMNDLRNEIR----IQN-MNPY-----PLEINMTVRISCVNCGKHFQYQL-TESAFTIAVSGSISNLSLQDSIDMIFQEQILNCYXXXXXXXXXXXXXXXXIRMRQPIASTLCVHVEKLIWNGRQNQFIFSKINFTDVLIMKLNYYENNSDRNSKLLSSC---NEIFLETAV---------------------------------------------KKELVYHLQSLISCQFNDK---YMCYKKLGNDWYCLTDTAMSNVSLNQVLESDPYILFYE 2113
SP SL +G N YL L S+SSCE + I+R+ K + K+ F NL+++ + +NA EIS N+I + WN + + F LL ++N + NEIR ++N ++ + P+++ + I C +CG L ++ A I+V + N +L+ +D F + I+ Y C+ K C+ K + ++ +P+ TLC+H++K WNG Q + I ++INF+ LI+K Y + +S+LL S + I A+ KK ++Y+L S+I + N K YMCYK + N WY D +S V V +SDPYIL YE
Sbjct: 55 SPPSLANIGANCYLNSALQSISSCEKFKEDIKRLHSKYKDLDKLTLFHNLMIVLNNGSKNAIEISRNNIFRHWNKSGQQDSFELLAYVINQVYNEIRNHSEVKNDISGWLRIRLPMKLLIQYSIKC-SCGTIISIPLHSDLALHISVPRN-GNETLERCLDQHFTDDIILNYICDTKSCAGLNALKMT-KLLRPLPQTLCIHLQKSYWNGYQMKKINNRINFSAELIIKSEYCHTIDEIDSELLKSISNNSSITFSKALSKSSDSEKSLIGKGISLQKSFPSIKDNVINNLRLTSINSGEHLKQKKNILYNLCSVIVHKGNVKGGHYMCYKCIDNQWYLADDNQISKVRKEIVFKSDPYILIYE 415 HSP 2 Score: 100.908 bits (250), Expect = 1.701e-22 Identity = 53/184 (28.80%), Postives = 113/184 (61.41%), Query Frame = 2
Query: 161 QSKSSCSDIVSKLTKLQSEYPNLEELNMLIELVEILQRDSEMKWKKSQNSIFKPRSKIDEQDNSDVLSFLINRMHHDIKTKIKIQKSCSNNLLNIKFPIIMTVKYDIICQCEKVIFSTVQSQISLNLTVS-SKYTNLQNLFDKIFLGEVVKGFNCETRNCSHKKAVRVCKLCKPLPETICIRLQ 709
QS SSC + +L S+Y +L++L + L+ +L S+ + S+N+IF+ +K +QD+ ++L+++IN+++++I+ +++ S L I+ P+ + ++Y I C C +I + S ++L+++V + L+ D+ F +++ + C+T++C+ A+++ KL +PLP+T+CI LQ
Sbjct: 73 QSISSCEKFKEDIKRLHSKYKDLDKLTLFHNLMIVLNNGSKNAIEISRNNIFRHWNKSGQQDSFELLAYVINQVYNEIRNHSEVKNDIS-GWLRIRLPMKLLIQYSIKCSCGTIISIPLHSDLALHISVPRNGNETLERCLDQHFTDDIILNYICDTKSCAGLNALKMTKLLRPLPQTLCIHLQ 255
BLAST of SMED30034099 vs. Planmine SMEST
Match: SMESG000040010.1 (SMESG000040010.1) HSP 1 Score: 108.612 bits (270), Expect = 4.969e-25 Identity = 110/364 (30.22%), Postives = 176/364 (48.35%), Query Frame = 2
Query: 1220 SPVSLKKLGINQYLGICLHSLSSCEVLIKRIQRITQK--SMRKMKTFINLIMISDGDYRNA-EIS-NSIIKEWNALYKTNLFHLLLALMNDLRNEIR----IQN-MNPY-----PLEINMTVRISCVNCGKHFQYQL-TESAFTIAVSGSISNLSLQDSIDMIFQEQILNCYXXXXXXXXXXXXXXXXIRMRQPIASTLCVHVEKLIWNGRQNQFIFSKINFTDVLIMKLNYYENNSDRNSKLLSSC---NEIFLETAV---------------------------------------------KKELVYHLQSLISCQFNDK---YMCYKKLGNDWYCLTDTAMSNVSLNQVLESDPYILFYE 2113
SP SL +G N YL L S+SSCE + I+R+ K + K+ F NL+++ + +NA EIS N+I + WN + + F LL ++N + NEIR ++N ++ + P+++ + I C +CG L ++ A I+V + N +L+ +D F + I+ Y C+ K C+ K + ++ +P+ TLC+H++K WNG Q + I ++INF+ LI+K Y + +S+LL S + I A+ KK ++Y+L S+I + N K YMCYK + N WY D +S V V +SDPYIL YE
Sbjct: 53 SPPSLANIGANCYLNSALQSISSCEKFKEDIKRLHSKYKDLDKLTLFHNLMIVLNNGSKNAIEISRNNIFRHWNKSGQQDSFELLAYVINQVYNEIRNHSEVKNDISGWLRIRLPMKLLIQYSIKC-SCGTIISIPLHSDLALHISVPRN-GNETLERCLDQHFTDDIILNYICDTKSCAGLNALKMT-KLLRPLPQTLCIHLQKSYWNGYQMKKINNRINFSAELIIKSEYCHTIDEIDSELLKSISNNSSITFSKALSKSSDSEKSLIGKGISLQKSFPSIKDNVINNLRLTSINSGEHLKQKKNILYNLCSVIVHKGNVKGGHYMCYKCIDNQWYLADDNQISKVRKEIVFKSDPYILIYE 413 HSP 2 Score: 101.293 bits (251), Expect = 1.452e-22 Identity = 53/184 (28.80%), Postives = 113/184 (61.41%), Query Frame = 2
Query: 161 QSKSSCSDIVSKLTKLQSEYPNLEELNMLIELVEILQRDSEMKWKKSQNSIFKPRSKIDEQDNSDVLSFLINRMHHDIKTKIKIQKSCSNNLLNIKFPIIMTVKYDIICQCEKVIFSTVQSQISLNLTVS-SKYTNLQNLFDKIFLGEVVKGFNCETRNCSHKKAVRVCKLCKPLPETICIRLQ 709
QS SSC + +L S+Y +L++L + L+ +L S+ + S+N+IF+ +K +QD+ ++L+++IN+++++I+ +++ S L I+ P+ + ++Y I C C +I + S ++L+++V + L+ D+ F +++ + C+T++C+ A+++ KL +PLP+T+CI LQ
Sbjct: 71 QSISSCEKFKEDIKRLHSKYKDLDKLTLFHNLMIVLNNGSKNAIEISRNNIFRHWNKSGQQDSFELLAYVINQVYNEIRNHSEVKNDIS-GWLRIRLPMKLLIQYSIKCSCGTIISIPLHSDLALHISVPRNGNETLERCLDQHFTDDIILNYICDTKSCAGLNALKMTKLLRPLPQTLCIHLQ 253 The following BLAST results are available for this feature:
BLAST of SMED30034099 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30034099 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30034099 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of SMED30034099 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30034099 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30034099 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30034099 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30034099 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30034099 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30034099 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30034099 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30034099 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30034099 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30034099 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30034099 ID=SMED30034099|Name=SMED30034099|organism=Schmidtea mediterranea sexual|type=transcript|length=2133bpback to top protein sequence of SMED30034099-orf-1 >SMED30034099-orf-1 ID=SMED30034099-orf-1|Name=SMED30034099-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=680bp MGQLQSFFNLLAKDESLYSFDSTMNQSKSSCSDIVSKLTKLQSEYPNLEEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
