Eukaryotic peptide chain release factor subunit 1

Overview


Name	Eukaryotic peptide chain release factor subunit 1
Smed ID	SMED30034032
Length (bp)	1546

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Eukaryotic peptide chain release factor subunit 1 (SMED30034032) t-SNE clustered cells

Violin plots show distribution of expression levels for Eukaryotic peptide chain release factor subunit 1 (SMED30034032) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Eukaryotic peptide chain release factor subunit 1 (SMED30034032) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30034032

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
nervous system	SMED30034032	h1SMcG0001170	dd_Smed_v4_5017_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30034032	h1SMcG0001170	dd_Smed_v4_5017_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30034032	h1SMcG0001170	dd_Smed_v4_5017_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

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Homology

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Human
Match: ETF1 (eukaryotic translation termination factor 1 [Source:HGNC Symbol;Acc:HGNC:3477])

HSP 1 Score: 649.818 bits (1675), Expect = 0.000e+0
Identity = 321/436 (73.62%), Postives = 376/436 (86.24%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FIS DKVNV GLVLAGSADFK EL +SD+FD RL +KVLK VD+SYGGE GFNQAIE+S + L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL + RY L    T E   L L PEQ+K+++HF DK T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+      ++E F++DDY
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISGDKVNVAGLVLAGSADFKTELSQSDMFDQRLQSKVLKLVDISYGGENGFNQAIELSTEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDIMRYVLHCQGTEEEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQ--GGDDEFFDLDDY 437

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Human
Match: ETF1 (eukaryotic translation termination factor 1 [Source:HGNC Symbol;Acc:HGNC:3477])

HSP 1 Score: 610.527 bits (1573), Expect = 0.000e+0
Identity = 302/410 (73.66%), Postives = 353/410 (86.10%), Query Frame = 3

Query:  156 NGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            NGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FIS DKVNV GLVLAGSADFK EL +SD+FD RL +KVLK VD+SYGGE GFNQAIE+S + L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL + RY L    T E   L L PEQ+K+++HF DK T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+      ++E F++DDY
Sbjct:   16 NGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISGDKVNVAGLVLAGSADFKTELSQSDMFDQRLQSKVLKLVDISYGGENGFNQAIELSTEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDIMRYVLHCQGTEEEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQ--GGDDEFFDLDDY 423

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Human
Match: ETF1 (eukaryotic translation termination factor 1 [Source:HGNC Symbol;Acc:HGNC:3477])

HSP 1 Score: 602.438 bits (1552), Expect = 0.000e+0
Identity = 298/406 (73.40%), Postives = 349/406 (85.96%), Query Frame = 3

Query:  168 MISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            MISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FIS DKVNV GLVLAGSADFK EL +SD+FD RL +KVLK VD+SYGGE GFNQAIE+S + L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL + RY L    T E   L L PEQ+K+++HF DK T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+      ++E F++DDY
Sbjct:    1 MISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISGDKVNVAGLVLAGSADFKTELSQSDMFDQRLQSKVLKLVDISYGGENGFNQAIELSTEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDIMRYVLHCQGTEEEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQ--GGDDEFFDLDDY 404

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Human
Match: ETF1 (eukaryotic translation termination factor 1 [Source:HGNC Symbol;Acc:HGNC:3477])

HSP 1 Score: 376.326 bits (965), Expect = 2.461e-129
Identity = 176/208 (84.62%), Postives = 197/208 (94.71%), Query Frame = 3

Query:  168 MISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDI 791
            MISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FIS DKVNV GLVLAGSADFK EL +SD+
Sbjct:    1 MISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISGDKVNVAGLVLAGSADFKTELSQSDM 208

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Human
Match: ETF1 (eukaryotic translation termination factor 1 [Source:HGNC Symbol;Acc:HGNC:3477])

HSP 1 Score: 214.927 bits (546), Expect = 1.095e-67
Identity = 99/119 (83.19%), Postives = 113/119 (94.96%), Query Frame = 3

Query:  168 MISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVD 524
            MISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+D
Sbjct:    1 MISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVID 119

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Celegans
Match: erfa-1 (Eukaryotic peptide chain release factor subunit 1 [Source:UniProtKB/Swiss-Prot;Acc:O16520])

HSP 1 Score: 605.52 bits (1560), Expect = 0.000e+0
Identity = 294/429 (68.53%), Postives = 360/429 (83.92%), Query Frame = 3

Query:   99 NVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            NVEMWKIK+LIK+LE ARGNGTSMISLII P DQ+ ++ +MLA+EYGTASNIKSRVNRLSVL AITS Q +LKLYN+VP NGLVVYCGTIMTD+GKEKKV+IDFEPF+ +NTSLYLCDNKFHTEAL  LL D++KFGFI++DGNG LFGTL GNTR++LHKFTVDLPKKHGRGGQSA+RFARLR EKRHNYVRKVAE +V+ FI NDKV V GL+LAGSADFK EL +SD+FD RL AK++K VD++YGGE GFNQAIE++AD+LA+VKFIQE+KLI  +FDEI Q+TGK+ FG+ DT+  LEMGA+ETLI WENL + RY ++ NS GE   L LRP+++K+++HF D  T  ++E++E    LE+    YK++GA LEIV+DKS EG+QFV+GFGGIGG+LRY+VD   +D    E E ++ ++DDY
Sbjct:   19 NVEMWKIKRLIKSLELARGNGTSMISLIIPPKDQVARIQRMLAEEYGTASNIKSRVNRLSVLGAITSVQGRLKLYNKVPPNGLVVYCGTIMTDEGKEKKVNIDFEPFKAINTSLYLCDNKFHTEALQGLLADDNKFGFIIMDGNGCLFGTLQGNTREVLHKFTVDLPKKHGRGGQSAVRFARLRNEKRHNYVRKVAENSVEQFIKNDKVTVAGLILAGSADFKTELGQSDMFDQRLQAKMIKTVDIAYGGENGFNQAIELAADTLASVKFIQEKKLIGGYFDEISQDTGKYVFGVKDTLAALEMGAIETLICWENLDIVRYKMK-NSLGEDILLNLRPDEEKDKSHFTDSETGQDMEIIETMPLLEWFANNYKNFGAALEIVTDKSQEGAQFVRGFGGIGGLLRYRVDLAHVD---LEDELDNIDLDDY 443

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Celegans
Match: erfa-1 (Eukaryotic peptide chain release factor subunit 1 [Source:UniProtKB/Swiss-Prot;Acc:O16520])

HSP 1 Score: 605.134 bits (1559), Expect = 0.000e+0
Identity = 294/429 (68.53%), Postives = 360/429 (83.92%), Query Frame = 3

Query:   99 NVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            NVEMWKIK+LIK+LE ARGNGTSMISLII P DQ+ ++ +MLA+EYGTASNIKSRVNRLSVL AITS Q +LKLYN+VP NGLVVYCGTIMTD+GKEKKV+IDFEPF+ +NTSLYLCDNKFHTEAL  LL D++KFGFI++DGNG LFGTL GNTR++LHKFTVDLPKKHGRGGQSA+RFARLR EKRHNYVRKVAE +V+ FI NDKV V GL+LAGSADFK EL +SD+FD RL AK++K VD++YGGE GFNQAIE++AD+LA+VKFIQE+KLI  +FDEI Q+TGK+ FG+ DT+  LEMGA+ETLI WENL + RY ++ NS GE   L LRP+++K+++HF D  T  ++E++E    LE+    YK++GA LEIV+DKS EG+QFV+GFGGIGG+LRY+VD   +D    E E ++ ++DDY
Sbjct:   19 NVEMWKIKRLIKSLELARGNGTSMISLIIPPKDQVARIQRMLAEEYGTASNIKSRVNRLSVLGAITSVQGRLKLYNKVPPNGLVVYCGTIMTDEGKEKKVNIDFEPFKAINTSLYLCDNKFHTEALQGLLADDNKFGFIIMDGNGCLFGTLQGNTREVLHKFTVDLPKKHGRGGQSAVRFARLRNEKRHNYVRKVAENSVEQFIKNDKVTVAGLILAGSADFKTELGQSDMFDQRLQAKMIKTVDIAYGGENGFNQAIELAADTLASVKFIQEKKLIGGYFDEISQDTGKYVFGVKDTLAALEMGAIETLICWENLDIVRYKMK-NSLGEDILLNLRPDEEKDKSHFTDSETGQDMEIIETMPLLEWFANNYKNFGAALEIVTDKSQEGAQFVRGFGGIGGLLRYRVDLAHVD---LEDELDNIDLDDY 443

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Celegans
Match: erfa-1 (Eukaryotic peptide chain release factor subunit 1 [Source:UniProtKB/Swiss-Prot;Acc:O16520])

HSP 1 Score: 605.134 bits (1559), Expect = 0.000e+0
Identity = 294/429 (68.53%), Postives = 360/429 (83.92%), Query Frame = 3

Query:   99 NVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            NVEMWKIK+LIK+LE ARGNGTSMISLII P DQ+ ++ +MLA+EYGTASNIKSRVNRLSVL AITS Q +LKLYN+VP NGLVVYCGTIMTD+GKEKKV+IDFEPF+ +NTSLYLCDNKFHTEAL  LL D++KFGFI++DGNG LFGTL GNTR++LHKFTVDLPKKHGRGGQSA+RFARLR EKRHNYVRKVAE +V+ FI NDKV V GL+LAGSADFK EL +SD+FD RL AK++K VD++YGGE GFNQAIE++AD+LA+VKFIQE+KLI  +FDEI Q+TGK+ FG+ DT+  LEMGA+ETLI WENL + RY ++ NS GE   L LRP+++K+++HF D  T  ++E++E    LE+    YK++GA LEIV+DKS EG+QFV+GFGGIGG+LRY+VD   +D    E E ++ ++DDY
Sbjct:   19 NVEMWKIKRLIKSLELARGNGTSMISLIIPPKDQVARIQRMLAEEYGTASNIKSRVNRLSVLGAITSVQGRLKLYNKVPPNGLVVYCGTIMTDEGKEKKVNIDFEPFKAINTSLYLCDNKFHTEALQGLLADDNKFGFIIMDGNGCLFGTLQGNTREVLHKFTVDLPKKHGRGGQSAVRFARLRNEKRHNYVRKVAENSVEQFIKNDKVTVAGLILAGSADFKTELGQSDMFDQRLQAKMIKTVDIAYGGENGFNQAIELAADTLASVKFIQEKKLIGGYFDEISQDTGKYVFGVKDTLAALEMGAIETLICWENLDIVRYKMK-NSLGEDILLNLRPDEEKDKSHFTDSETGQDMEIIETMPLLEWFANNYKNFGAALEIVTDKSQEGAQFVRGFGGIGGLLRYRVDLAHVD---LEDELDNIDLDDY 443

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Fly
Match: eRF1 (gene:FBgn0036974 transcript:FBtr0078174)

HSP 1 Score: 641.343 bits (1653), Expect = 0.000e+0
Identity = 313/436 (71.79%), Postives = 379/436 (86.93%), Query Frame = 3

Query:   81 ESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNS-TGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            E+ +D NVE+WKIKKLIK+LE ARGNGTSMISLII P DQI++V+KMLADE+GTASNIKSRVNRLSVL AITS Q +LKLY +VP NGLV+YCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D++KFGFIV+DGNGALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAE A Q+FI+NDK N+ GL+LAGSADFK EL +SD+FD RL +KV+K VDVSYGGE GFNQAIE++A+SL NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT+R LE+G+VETLI WENL + RY L+N++ +  TT L L PEQ+K+++HF DK +  E+EL+E Q  LE+L   YK +GA LEI++DKS EGSQFV+GFGGIGGILRYKVDFQ M  D  E + + F++DDY
Sbjct:    5 ETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFITNDKPNIAGLILAGSADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHANSTSTTVLHLTPEQEKDKSHFTDKESGVEMELIESQPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQSMQLD--ELDNDGFDLDDY 438

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Fly
Match: eRF1 (gene:FBgn0036974 transcript:FBtr0078178)

HSP 1 Score: 641.343 bits (1653), Expect = 0.000e+0
Identity = 313/436 (71.79%), Postives = 379/436 (86.93%), Query Frame = 3

Query:   81 ESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNS-TGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            E+ +D NVE+WKIKKLIK+LE ARGNGTSMISLII P DQI++V+KMLADE+GTASNIKSRVNRLSVL AITS Q +LKLY +VP NGLV+YCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D++KFGFIV+DGNGALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAE A Q+FI+NDK N+ GL+LAGSADFK EL +SD+FD RL +KV+K VDVSYGGE GFNQAIE++A+SL NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT+R LE+G+VETLI WENL + RY L+N++ +  TT L L PEQ+K+++HF DK +  E+EL+E Q  LE+L   YK +GA LEI++DKS EGSQFV+GFGGIGGILRYKVDFQ M  D  E + + F++DDY
Sbjct:    5 ETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFITNDKPNIAGLILAGSADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHANSTSTTVLHLTPEQEKDKSHFTDKESGVEMELIESQPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQSMQLD--ELDNDGFDLDDY 438

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Fly
Match: eRF1 (gene:FBgn0036974 transcript:FBtr0078173)

HSP 1 Score: 641.343 bits (1653), Expect = 0.000e+0
Identity = 313/436 (71.79%), Postives = 379/436 (86.93%), Query Frame = 3

Query:   81 ESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNS-TGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            E+ +D NVE+WKIKKLIK+LE ARGNGTSMISLII P DQI++V+KMLADE+GTASNIKSRVNRLSVL AITS Q +LKLY +VP NGLV+YCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D++KFGFIV+DGNGALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAE A Q+FI+NDK N+ GL+LAGSADFK EL +SD+FD RL +KV+K VDVSYGGE GFNQAIE++A+SL NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT+R LE+G+VETLI WENL + RY L+N++ +  TT L L PEQ+K+++HF DK +  E+EL+E Q  LE+L   YK +GA LEI++DKS EGSQFV+GFGGIGGILRYKVDFQ M  D  E + + F++DDY
Sbjct:    5 ETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFITNDKPNIAGLILAGSADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHANSTSTTVLHLTPEQEKDKSHFTDKESGVEMELIESQPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQSMQLD--ELDNDGFDLDDY 438

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Fly
Match: eRF1 (gene:FBgn0036974 transcript:FBtr0078175)

HSP 1 Score: 641.343 bits (1653), Expect = 0.000e+0
Identity = 313/436 (71.79%), Postives = 379/436 (86.93%), Query Frame = 3

Query:   81 ESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNS-TGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            E+ +D NVE+WKIKKLIK+LE ARGNGTSMISLII P DQI++V+KMLADE+GTASNIKSRVNRLSVL AITS Q +LKLY +VP NGLV+YCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D++KFGFIV+DGNGALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAE A Q+FI+NDK N+ GL+LAGSADFK EL +SD+FD RL +KV+K VDVSYGGE GFNQAIE++A+SL NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT+R LE+G+VETLI WENL + RY L+N++ +  TT L L PEQ+K+++HF DK +  E+EL+E Q  LE+L   YK +GA LEI++DKS EGSQFV+GFGGIGGILRYKVDFQ M  D  E + + F++DDY
Sbjct:    5 ETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFITNDKPNIAGLILAGSADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHANSTSTTVLHLTPEQEKDKSHFTDKESGVEMELIESQPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQSMQLD--ELDNDGFDLDDY 438

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Fly
Match: eRF1 (gene:FBgn0036974 transcript:FBtr0078177)

HSP 1 Score: 641.343 bits (1653), Expect = 0.000e+0
Identity = 313/436 (71.79%), Postives = 379/436 (86.93%), Query Frame = 3

Query:   81 ESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNS-TGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            E+ +D NVE+WKIKKLIK+LE ARGNGTSMISLII P DQI++V+KMLADE+GTASNIKSRVNRLSVL AITS Q +LKLY +VP NGLV+YCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D++KFGFIV+DGNGALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAE A Q+FI+NDK N+ GL+LAGSADFK EL +SD+FD RL +KV+K VDVSYGGE GFNQAIE++A+SL NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT+R LE+G+VETLI WENL + RY L+N++ +  TT L L PEQ+K+++HF DK +  E+EL+E Q  LE+L   YK +GA LEI++DKS EGSQFV+GFGGIGGILRYKVDFQ M  D  E + + F++DDY
Sbjct:    5 ETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLADDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVATQLFITNDKPNIAGLILAGSADFKTELSQSDMFDPRLQSKVIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHANSTSTTVLHLTPEQEKDKSHFTDKESGVEMELIESQPLLEWLANNYKMFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQSMQLD--ELDNDGFDLDDY 438

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Zebrafish
Match: etf1b (eukaryotic translation termination factor 1b [Source:ZFIN;Acc:ZDB-GENE-021029-1])

HSP 1 Score: 649.432 bits (1674), Expect = 0.000e+0
Identity = 321/425 (75.53%), Postives = 372/425 (87.53%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLR---NNSTGETTE--LILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMD 1337
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+F+SNDKVNV GLVLAGSADFK EL +SD+FD RL AKVLK VD+SYGGE GFNQAIE+SA+ L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL   RY LR    N+T E  E  L L PEQ+K+++HF DK T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFVSNDKVNVAGLVLAGSADFKTELSQSDMFDPRLQAKVLKLVDISYGGENGFNQAIELSAEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDTMRYVLRCHGENTTPENDEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGME 428

[Close]

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLR---NNSTGETTE--LILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMD 1337
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+F+SNDKVNV GLVLAGSADFK EL +SD+FD RL AKVLK VD+SYGGE GFNQAIE+SA+ L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL   RY LR    N+T E  E  L L PEQ+K+++HF DK T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFVSNDKVNVAGLVLAGSADFKTELSQSDMFDPRLQAKVLKLVDISYGGENGFNQAIELSAEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDTMRYVLRCHGENTTPENDEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGME 428

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Zebrafish
Match: etf1b (eukaryotic translation termination factor 1b [Source:ZFIN;Acc:ZDB-GENE-021029-1])

HSP 1 Score: 641.343 bits (1653), Expect = 0.000e+0
Identity = 315/435 (72.41%), Postives = 368/435 (84.60%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTE---------LILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXX------XXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMD 1337
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+F+SNDKVNV GLVLAGSADFK EL +SD+FD RL AKVLK VD+SYGGE GFNQAIE+SA+ L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL   RY LR +    T E         L L PEQ+K+++HF DK  N  +   +E   +E +         YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFVSNDKVNVAGLVLAGSADFKTELSQSDMFDPRLQAKVLKLVDISYGGENGFNQAIELSAEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDTMRYVLRCHGENTTPENGTHIYEKILYLTPEQEKDKSHFTDKEVNKIISTGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGME 438

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Zebrafish
Match: etf1a (eukaryotic translation termination factor 1a [Source:ZFIN;Acc:ZDB-GENE-080218-14])

HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0
Identity = 294/425 (69.18%), Postives = 354/425 (83.29%), Query Frame = 3

Query:  156 NGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLR------NNSTGETTELILRPEQKKERAHFLDK---------TTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            NGTSMISLI+ P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLY++VP NGLVVYCGTI+TD+GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNT+++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+++SNDKVNV GLVLAGSADFK EL +SD+FD RL  KVLK VD+ YGGE GFNQAIE+SA+ L NVKF+QE+KLI ++FDEI Q+TGK+C+G++DT+R LEMGAVE LIV+EN    RY L+      N S  +   + L PEQ+++++HF+DK          T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDF G++   +++EEE F++ DY
Sbjct:    1 NGTSMISLIVPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYSKVPHNGLVVYCGTIVTDEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLTDDSKFGFIVIDGSGALFGTLQGNTKEVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLYVSNDKVNVVGLVLAGSADFKTELSQSDMFDPRLQVKVLKLVDICYGGENGFNQAIELSAEILINVKFMQEKKLIGRYFDEISQDTGKYCYGVEDTLRALEMGAVEILIVYENQETMRYVLKLHGAELNGSKNDEKVIYLSPEQERDKSHFIDKEECVLLGCFQTGHEHELMECMPLLEWFASNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFHGLE---YQEEEELFDLGDY 422

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Xenopus
Match: etf1 (eukaryotic translation termination factor 1 [Source:NCBI gene;Acc:394632])

HSP 1 Score: 655.596 bits (1690), Expect = 0.000e+0
Identity = 322/436 (73.85%), Postives = 379/436 (86.93%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FIS DKVNV GLVLAGSADFK EL +SD+FD RL +KVLK VD+SYGGE GFNQAIE+S + L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL + RY LR N + E   L L PEQ+K+++HF+DK T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+      ++E F++DDY
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISGDKVNVAGLVLAGSADFKTELSQSDMFDQRLQSKVLKLVDISYGGENGFNQAIELSTEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDIMRYVLRCNGSEEEKTLYLTPEQEKDKSHFIDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQ--GGDDEFFDLDDY 437

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Xenopus
Match: etf1 (eukaryotic translation termination factor 1 [Source:NCBI gene;Acc:394632])

HSP 1 Score: 646.351 bits (1666), Expect = 0.000e+0
Identity = 322/446 (72.20%), Postives = 379/446 (84.98%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDK----------TTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FIS DKVNV GLVLAGSADFK EL +SD+FD RL +KVLK VD+SYGGE GFNQAIE+S + L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL + RY LR N + E   L L PEQ+K+++HF+DK           T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+      ++E F++DDY
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISGDKVNVAGLVLAGSADFKTELSQSDMFDQRLQSKVLKLVDISYGGENGFNQAIELSTEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDIMRYVLRCNGSEEEKTLYLTPEQEKDKSHFIDKEVCAHVLGCFQTGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQ--GGDDEFFDLDDY 447

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Mouse
Match: Etf1 (eukaryotic translation termination factor 1 [Source:MGI Symbol;Acc:MGI:2385071])

HSP 1 Score: 649.818 bits (1675), Expect = 0.000e+0
Identity = 321/436 (73.62%), Postives = 376/436 (86.24%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FIS DKVNV GLVLAGSADFK EL +SD+FD RL +KVLK VD+SYGGE GFNQAIE+S + L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL + RY L    T E   L L PEQ+K+++HF DK T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+      ++E F++DDY
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISGDKVNVAGLVLAGSADFKTELSQSDMFDQRLQSKVLKLVDISYGGENGFNQAIELSTEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDIMRYVLHCQGTEEEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQ--GGDDEFFDLDDY 437

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. UniProt/SwissProt
Match: sp|P35615|ERF1_XENLA (Eukaryotic peptide chain release factor subunit 1 OS=Xenopus laevis OX=8355 GN=etf1 PE=2 SV=1)

HSP 1 Score: 657.136 bits (1694), Expect = 0.000e+0
Identity = 323/436 (74.08%), Postives = 378/436 (86.70%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FIS DKVNV GLVLAGSADFK EL +SD+FD RL +KVLK VD+SYGGE GFNQAIE+S + L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL   RY LR N + E   L L PEQ+K+++HF+DK T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGMD      ++E F++DDY
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISGDKVNVAGLVLAGSADFKTELSQSDMFDQRLQSKVLKLVDISYGGENGFNQAIELSTEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDTMRYVLRCNGSEEEKTLYLTPEQEKDKSHFIDKETGQEHELIESMPLLEWFANSYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMDYQGV--DDEFFDLDDY 437

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. UniProt/SwissProt
Match: sp|P62498|ERF1_XENTR (Eukaryotic peptide chain release factor subunit 1 OS=Xenopus tropicalis OX=8364 GN=etf1 PE=2 SV=1)

HSP 1 Score: 655.596 bits (1690), Expect = 0.000e+0
Identity = 322/436 (73.85%), Postives = 379/436 (86.93%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FIS DKVNV GLVLAGSADFK EL +SD+FD RL +KVLK VD+SYGGE GFNQAIE+S + L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL + RY LR N + E   L L PEQ+K+++HF+DK T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+      ++E F++DDY
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISGDKVNVAGLVLAGSADFKTELSQSDMFDQRLQSKVLKLVDISYGGENGFNQAIELSTEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDIMRYVLRCNGSEEEKTLYLTPEQEKDKSHFIDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQ--GGDDEFFDLDDY 437

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. UniProt/SwissProt
Match: sp|Q9GR88|ERF1_POLMI (Eukaryotic peptide chain release factor subunit 1 OS=Polyandrocarpa misakiensis OX=7723 GN=ERF1 PE=2 SV=1)

HSP 1 Score: 651.744 bits (1680), Expect = 0.000e+0
Identity = 312/419 (74.46%), Postives = 371/419 (88.54%), Query Frame = 3

Query:   81 ESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMD 1337
            E+ +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQ+++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+TD+GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D++KFGFIV+DGNGALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FI+ND+ NV GLVLAGSADFK EL +SD+FD RL  K+LK VDVSYGGE GFNQAIE+SA+ L+NVKFIQE+KLI ++FDEI Q+TGK+CFG+DDT++ LEMGAVE LIVWENL   RY L+N++T E   L L+P+Q+K++ HF DK T  E+E++E    LE+    YK +G+ LEIV+D+S EGSQFVKGFGGIGG+LRY++DFQGM+
Sbjct:    5 ETTADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQVSRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTDEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDNKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFITNDRPNVTGLVLAGSADFKTELSQSDMFDQRLQVKILKLVDVSYGGENGFNQAIELSAEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVDDTLKALEMGAVEILIVWENLDTMRYVLKNHTTDEEKILFLKPDQEKDKTHFTDKDTGVEMEMVESCSLLEWFANNYKKFGSTLEIVTDRSQEGSQFVKGFGGIGGMLRYRIDFQGME 423

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. UniProt/SwissProt
Match: sp|Q5U2Q7|ERF1_RAT (Eukaryotic peptide chain release factor subunit 1 OS=Rattus norvegicus OX=10116 GN=Etf1 PE=2 SV=3)

HSP 1 Score: 649.818 bits (1675), Expect = 0.000e+0
Identity = 321/436 (73.62%), Postives = 376/436 (86.24%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FIS DKVNV GLVLAGSADFK EL +SD+FD RL +KVLK VD+SYGGE GFNQAIE+S + L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL + RY L    T E   L L PEQ+K+++HF DK T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+      ++E F++DDY
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISGDKVNVAGLVLAGSADFKTELSQSDMFDQRLQSKVLKLVDISYGGENGFNQAIELSTEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDIMRYVLHCQGTEEEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQ--GGDDEFFDLDDY 437

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. UniProt/SwissProt
Match: sp|P62497|ERF1_RABIT (Eukaryotic peptide chain release factor subunit 1 OS=Oryctolagus cuniculus OX=9986 GN=ETF1 PE=1 SV=3)

HSP 1 Score: 649.818 bits (1675), Expect = 0.000e+0
Identity = 321/436 (73.62%), Postives = 376/436 (86.24%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FIS DKVNV GLVLAGSADFK EL +SD+FD RL +KVLK VD+SYGGE GFNQAIE+S + L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL + RY L    T E   L L PEQ+K+++HF DK T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+      ++E F++DDY
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISGDKVNVAGLVLAGSADFKTELSQSDMFDQRLQSKVLKLVDISYGGENGFNQAIELSTEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDIMRYVLHCQGTEEEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQ--GGDDEFFDLDDY 437

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. TrEMBL
Match: A0A1I8JEF3 (eRF1_1 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030584g1 PE=4 SV=1)

HSP 1 Score: 672.544 bits (1734), Expect = 0.000e+0
Identity = 330/441 (74.83%), Postives = 393/441 (89.12%), Query Frame = 3

Query:   69 ANTNESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEE--EDFNIDDY 1385
            AN + S SD NVEMWKIKKLIK+LEAARGNGTSMISLII P DQIN+V+KMLADEYGTASNIKSRVNRLSVLSAI+S QQ+LKLY++VPTNGLVVYCGTI+T++GKEKKV+IDFEPFRP+NTSLYLCDNKFHTEAL+ALL D+S+FGFIV+DGNGALFGTL+GNTRD+LHKFTVDLPKKHGRGGQSALRFARLR EKRHNYVRKVAETAVQ+FI+ND+VN EGLVLAGSADFK EL +SD+FD RL +KVLK VD+SYGGE GFNQAIE+SAD+LANVKFIQE+KLI+++FDEI QNT K+CFG+ DT+  LE GAVETLIVWENL + RY LRN++    T L LRPEQ+K+++HF+DK T  ELEL++ +LFLE+LT  YK +GA +EIV+D+S EG+QFVKGFGGIGGILRY++DFQ ++  + +  +  ++F++DDY
Sbjct:    7 ANGDSSSSDRNVEMWKIKKLIKSLEAARGNGTSMISLIIPPKDQINRVSKMLADEYGTASNIKSRVNRLSVLSAISSVQQRLKLYSKVPTNGLVVYCGTIVTEEGKEKKVNIDFEPFRPINTSLYLCDNKFHTEALTALLADDSRFGFIVMDGNGALFGTLSGNTRDVLHKFTVDLPKKHGRGGQSALRFARLRQEKRHNYVRKVAETAVQLFITNDRVNCEGLVLAGSADFKTELSQSDMFDQRLQSKVLKVVDISYGGENGFNQAIELSADALANVKFIQEKKLIQRYFDEISQNTSKYCFGVTDTLHALESGAVETLIVWENLDITRYLLRNHTADVETVLHLRPEQEKDKSHFIDKETGVELELVDSKLFLEWLTETYKTFGATMEIVTDRSQEGAQFVKGFGGIGGILRYQLDFQELEMALAQNGDMYDNFDLDDY 447

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. TrEMBL
Match: A0A1S3H0N4 (eukaryotic peptide chain release factor subunit 1 OS=Lingula unguis OX=7574 GN=LOC106151134 PE=4 SV=1)

HSP 1 Score: 664.455 bits (1713), Expect = 0.000e+0
Identity = 325/441 (73.70%), Postives = 390/441 (88.44%), Query Frame = 3

Query:   63 MSANTNESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            MSAN  E+ +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVLSAITS QQ+LKLY +VP NGLV+YCGTI+TDDGKE+KV+IDFEPFRP+NTSLYLCDNKFHTEAL+ALLED++KFGFI++DGNGALFGTL+GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAV +FI+NDK NV GLVLAGSADFK EL +SD+FD RL  K++K VDVSYGGE GFNQAIE+++DSL+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMG+VETLIVWENL + RY L+N+ST E   L L+PEQ+K++ +F DK T  ELEL + Q  LE+L   YK +GA LEI++D+S EG+QFVKGFGGIGGILRY+VDFQ M+  M + E +D+++DDY
Sbjct:    1 MSAN-GETSADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLSAITSVQQRLKLYTKVPPNGLVIYCGTIVTDDGKERKVNIDFEPFRPINTSLYLCDNKFHTEALTALLEDDNKFGFIIMDGNGALFGTLSGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVTLFITNDKPNVAGLVLAGSADFKTELSQSDMFDPRLQCKIIKVVDVSYGGENGFNQAIELASDSLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGSVETLIVWENLDITRYVLKNHSTDEEKILHLKPEQEKDKTYFTDKDTGVELELSDSQPLLEWLANNYKTFGATLEIITDRSQEGAQFVKGFGGIGGILRYQVDFQMMEGPM-DGEFDDWDMDDY 439

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. TrEMBL
Match: V4AI12 (eRF1_1 domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_216289 PE=4 SV=1)

HSP 1 Score: 664.07 bits (1712), Expect = 0.000e+0
Identity = 320/435 (73.56%), Postives = 385/435 (88.51%), Query Frame = 3

Query:   81 ESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            E+ +D NVEMWKIKKLIK+LEAARGNGTSMISLII P DQ+++V KMLADEYGTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLV+YCGTI+T++GKEKKV+IDFEPFRP+NTSLYLCDNKFHTEAL+ALL D++KFGFI++DGNGALFGTL+GNTR+ILHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAE AVQ+FI+NDK NV GLVLAGSADFK EL +SD+FD RL AK+LK VDVSYGGE GFNQAIE++ADSLANVKFIQE+KLI ++FDEI Q+TGK+CFG++DT+R LEMGAV+TLIVWENL++ R+ +R++ST E   L L+P+Q+ ++ +F DK T  E+E++E+   LE+    YK++GA LEI++DKS EG+QFV+GFGGIGGILRY+VDFQ    D+  QE +DF+IDDY
Sbjct:    9 ETSADRNVEMWKIKKLIKSLEAARGNGTSMISLIIPPKDQVSRVNKMLADEYGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVIYCGTIITEEGKEKKVNIDFEPFRPINTSLYLCDNKFHTEALTALLADDNKFGFIIMDGNGALFGTLSGNTREILHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAEVAVQLFITNDKPNVAGLVLAGSADFKTELSQSDMFDQRLQAKILKIVDVSYGGENGFNQAIELAADSLANVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALEMGAVDTLIVWENLSITRFVVRDHSTEENRILFLQPDQESDKTNFTDKDTGVEMEMVEQVSLLEWFANNYKNFGATLEIITDKSQEGAQFVRGFGGIGGILRYQVDFQMFHPDL--QEYDDFDIDDY 441

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. TrEMBL
Match: A0A0R3UQ50 (eRF1_1 domain-containing protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS9998 PE=4 SV=3)

HSP 1 Score: 663.3 bits (1710), Expect = 0.000e+0
Identity = 325/447 (72.71%), Postives = 394/447 (88.14%), Query Frame = 3

Query:   63 MSANTNESGS---DNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGM---DTDMFEQEEEDFNIDDY 1385
            MSA+  E+ +   D NVEMW+IKKLIK+LEAARGNGTSMISLII P DQI +VAKMLA+EYGTASNIKSRVNRLSVLSAITS QQ+LKLYN+VP NGLVVYCGTI+T+DGKEKKV+IDFEPFRPVNTSLYLCDNKFHTEAL+ALL D+SKFGFI++DG+G+LFGTL+GNTR++LHKF+V+LPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQ+FI+NDKVNVEG++LAG ADFK +L +S +FD RL AKVLK VD+SYGGE GFNQAIE+++D+LA VKFIQE+KLI+++FDEI Q+TGK+CFG+DDT++ LEMGAV++LIVWENL+V RY LR++S+ E   + LRP+Q+++R+HF+D  T  E+E+ E QL L++L L Y+ +GAVLEIV+DKS EGSQFV+GFGGIGGILRY+VDFQ M   D D     +EDF++ DY
Sbjct:    1 MSASNGETATTQADKNVEMWRIKKLIKSLEAARGNGTSMISLIIPPKDQITRVAKMLAEEYGTASNIKSRVNRLSVLSAITSVQQRLKLYNKVPPNGLVVYCGTIITEDGKEKKVNIDFEPFRPVNTSLYLCDNKFHTEALNALLTDDSKFGFIIMDGSGSLFGTLSGNTREVLHKFSVELPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQLFITNDKVNVEGIILAGLADFKTDLSQSGMFDPRLQAKVLKVVDISYGGESGFNQAIELASDTLAGVKFIQEKKLIQRYFDEISQDTGKYCFGVDDTLKALEMGAVDSLIVWENLSVTRYVLRSSSSDEKRIIHLRPDQERDRSHFIDPDTQTEMEVEESQLLLDWLALNYRSFGAVLEIVTDKSHEGSQFVRGFGGIGGILRYQVDFQHMAGGDLDY----DEDFDLSDY 443

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. TrEMBL
Match: S4RP18 (Eukaryotic translation termination factor 1a OS=Petromyzon marinus OX=7757 PE=4 SV=1)

HSP 1 Score: 660.218 bits (1702), Expect = 0.000e+0
Identity = 323/439 (73.58%), Postives = 382/439 (87.02%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLR-NNSTGETTE--LILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            + + +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++V+KMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DGNGALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FISNDK NV GL+LAGSADFK EL +SD+FD RL +KVLK VD+SYGGE GFNQAIE+S + L +VKFIQE+KLI K+F+EI Q+TGK+CFG+DDT+R LEMGAVE LIVWENL + RY LR N +  E  E  L L PEQ+K+++HF+DK T  E E++E    LE+    YK +GA LEI++DKS EGSQFV+GFGGIGGILRY+VDFQGMD   F+ ++E+FN+DDY
Sbjct:    4 DPTAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISNDKPNVVGLILAGSADFKTELSQSDMFDPRLQSKVLKIVDISYGGENGFNQAIELSQEVLTSVKFIQEKKLIGKYFEEISQDTGKYCFGVDDTLRALEMGAVEILIVWENLEITRYVLRCNPAEAEDPEKVLYLTPEQEKDKSHFIDKETGVEHEMVENMPLLEWFANNYKKFGATLEIITDKSQEGSQFVRGFGGIGGILRYRVDFQGMD---FQGDDEEFNLDDY 439

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Cavefish
Match: etf1b (eukaryotic peptide chain release factor subunit 1 [Source:NCBI gene;Acc:103028819])

HSP 1 Score: 647.892 bits (1670), Expect = 0.000e+0
Identity = 320/426 (75.12%), Postives = 371/426 (87.09%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETT------ELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMD 1337
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+F+SNDKVNV G+VLAGSADFK EL +SD+FD RL AKVLK VD+SYGGE GFNQAIE+SA+ L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL   RY LR + T  TT       L L PEQ+K+++HF DK T  E ELLE    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFVSNDKVNVAGMVLAGSADFKTELSQSDMFDPRLQAKVLKLVDISYGGENGFNQAIELSAEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDTMRYVLRCHGTEGTTLENDEKTLYLTPEQEKDKSHFTDKETGQEHELLESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGME 429

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Cavefish
Match: etf1a (eukaryotic peptide chain release factor subunit 1-like [Source:NCBI gene;Acc:103031395])

HSP 1 Score: 642.884 bits (1657), Expect = 0.000e+0
Identity = 316/426 (74.18%), Postives = 369/426 (86.62%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLR------NNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMD 1337
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+F+SNDKVNV G+VLAGSADFK EL +SD+FD RL AKVLK VD+SYGGE GFNQAIE+SA+ L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL   RY LR      N    +   + L PEQ+K+++HF DK T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFVSNDKVNVAGMVLAGSADFKTELSQSDMFDPRLQAKVLKLVDISYGGENGFNQAIELSAEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDTMRYILRVHGAELNGLENDEKTIYLTPEQEKDKSHFTDKETGQEHELIECMPLLEWFANSYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGME 429

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006985.1 (pep scaffold:Pmarinus_7.0:GL476537:382840:396315:-1 gene:ENSPMAG00000006286.1 transcript:ENSPMAT00000006985.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 660.218 bits (1702), Expect = 0.000e+0
Identity = 323/439 (73.58%), Postives = 382/439 (87.02%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLR-NNSTGETTE--LILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            + + +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++V+KMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DGNGALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FISNDK NV GL+LAGSADFK EL +SD+FD RL +KVLK VD+SYGGE GFNQAIE+S + L +VKFIQE+KLI K+F+EI Q+TGK+CFG+DDT+R LEMGAVE LIVWENL + RY LR N +  E  E  L L PEQ+K+++HF+DK T  E E++E    LE+    YK +GA LEI++DKS EGSQFV+GFGGIGGILRY+VDFQGMD   F+ ++E+FN+DDY
Sbjct:    4 DPTAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVMDGNGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFISNDKPNVVGLILAGSADFKTELSQSDMFDPRLQSKVLKIVDISYGGENGFNQAIELSQEVLTSVKFIQEKKLIGKYFEEISQDTGKYCFGVDDTLRALEMGAVEILIVWENLEITRYVLRCNPAEAEDPEKVLYLTPEQEKDKSHFIDKETGVEHEMVENMPLLEWFANNYKKFGATLEIITDKSQEGSQFVRGFGGIGGILRYRVDFQGMD---FQGDDEEFNLDDY 439

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Yeast
Match: SUP45 (Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p [Source:SGD;Acc:S000000347])

HSP 1 Score: 568.155 bits (1463), Expect = 0.000e+0
Identity = 277/430 (64.42%), Postives = 354/430 (82.33%), Query Frame = 3

Query:   93 DNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDD 1382
            + N+E+WK+KKL+++LE ARGNGTSMISL+I P  QI    KML DEYGTASNIKSRVNRLSVLSAITSTQQKLKLYN +P NGLV+YCG I+T+DGKEKKV+ D EP++P+NTSLYLCDNKFHTE LS LL+ + KFGFIV+DG G LFG+++GNTR +LHKFTVDLPKKHGRGGQSALRFARLR EKRHNYVRKVAE AVQ FI+NDKVNV+GL+LAGSADFK +L +S++FD RL  KV+  VDVSYGGE GFNQAIE+SA++LANVK++QE+KL++ +FDEI Q+TGKFC+G+DDT++ L++GAVE LIV+ENL   RYT ++    E  +    PE  K+++  +DK T  E++++ E+  +E+L   YK++GA LE ++DKS+EG+QFV GFGGIG +LRYKV+F+ +   + E E+E ++ D+
Sbjct:    6 EKNIEIWKVKKLVQSLEKARGNGTSMISLVIPPKGQIPLYQKMLTDEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNTLPKNGLVLYCGDIITEDGKEKKVTFDIEPYKPINTSLYLCDNKFHTEVLSELLQADDKFGFIVMDGQGTLFGSVSGNTRTVLHKFTVDLPKKHGRGGQSALRFARLREEKRHNYVRKVAEVAVQNFITNDKVNVKGLILAGSADFKTDLAKSELFDPRLACKVISIVDVSYGGENGFNQAIELSAEALANVKYVQEKKLLEAYFDEISQDTGKFCYGIDDTLKALDLGAVEKLIVFENLETIRYTFKDAEDNEVIKFA-EPEA-KDKSFAIDKATGQEMDVVSEEPLIEWLAANYKNFGATLEFITDKSSEGAQFVTGFGGIGAMLRYKVNFEQL---VDESEDEYYDEDE 430

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Nematostella
Match: EDO41505 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S428])

HSP 1 Score: 647.892 bits (1670), Expect = 0.000e+0
Identity = 320/441 (72.56%), Postives = 383/441 (86.85%), Query Frame = 3

Query:   63 MSANTNESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDY 1385
            MS    E+ +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVLSAITS QQ+LKLY++VP NGLVVYCGTI+TDDGKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D++KFGFIV+DGNGALFGTL GNTRD+LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+FI+NDK N+ G++LAGSADFK+EL +SD+FD RL AKVLK VDVSYGGE GFNQAIE++++ LANVKFIQE+KLI ++FDEI Q+TGK+CFG+ DT++ LEMGAV+ LIVWENL + R  LRN+ T E T L L PEQ+K++ HF+D  +  ELEL+++   LE+L   YK++GA LEI++DKS EG+QF KGFGGIGGILRY+VDFQ M+ D      ED++ ++Y
Sbjct:    1 MSGEGTEA-ADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLSAITSVQQRLKLYSKVPPNGLVVYCGTIVTDDGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALNALLADDNKFGFIVMDGNGALFGTLCGNTRDVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFITNDKPNITGMILAGSADFKSELSQSDMFDQRLQAKVLKLVDVSYGGENGFNQAIELASEVLANVKFIQEKKLIGRYFDEISQDTGKYCFGVADTLKALEMGAVDILIVWENLDIQRIVLRNHQTDEDTVLHLNPEQEKDKTHFIDSESGVELELVDKNPLLEWLANNYKNFGATLEIITDKSQEGAQFCKGFGGIGGILRYRVDFQSMEYD--GDPLEDYDDEEY 438

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Medaka
Match: etf1a (eukaryotic translation termination factor 1 [Source:NCBI gene;Acc:101172342])

HSP 1 Score: 646.736 bits (1667), Expect = 0.000e+0
Identity = 317/426 (74.41%), Postives = 370/426 (86.85%), Query Frame = 3

Query:   78 NESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLR------NNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMD 1337
            + S +D NVE+WKIKKLIK+LEAARGNGTSMISLII P DQI++VAKMLADE+GTASNIKSRVNRLSVL AITS QQ+LKLYN+VP NGLVVYCGTI+T++GKEKKV+IDFEPF+P+NTSLYLCDNKFHTEAL+ALL D+SKFGFIV+DG+GALFGTL GNTR++LHKFTVDLPKKHGRGGQSALRFARLR+EKRHNYVRKVAETAVQ+F+SNDKVNV G+VLAGSADFK EL +SD+FD RL AKVLK VD+SYGGE GFNQAIE+SA+ L+NVKFIQE+KLI ++FDEI Q+TGK+CFG++DT++ LEMGAVE LIV+ENL   RY LR      N +  +   L L PEQ+K+++HF DK T  E EL+E    LE+    YK +GA LEIV+DKS EGSQFVKGFGGIGGILRY+VDFQGM+
Sbjct:    4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFVSNDKVNVAGMVLAGSADFKTELSQSDMFDPRLQAKVLKLVDISYGGENGFNQAIELSAEVLSNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALEMGAVEILIVYENLDTMRYVLRVHGAESNGAENDEKTLYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGME 429

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BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Planmine SMEST
Match: SMESG000019227.1 (SMESG000019227.1)

HSP 1 Score: 870.152 bits (2247), Expect = 0.000e+0
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 3

Query:   63 MSANTNESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDYE 1388
            MSANTNESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAELELLEEQLFLEFLTLKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDYE
Sbjct:    1 MSANTNESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAELELLEEQLFLEFLTLKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDYE 442

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Planmine SMEST
Match: SMESG000038950.1 (SMESG000038950.1)

HSP 1 Score: 620.542 bits (1599), Expect = 0.000e+0
Identity = 301/433 (69.52%), Postives = 371/433 (85.68%), Query Frame = 3

Query:   90 SDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKVAKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDYE 1388
            +D N+E+WK+KKLI  L++ RGNGTSMIS+IIKPGDQI ++ KML DE GTASNIKSRVNRLSVLSAITS QQ+LKL N++PTNGLVVYCGTIM DDGKEKK SIDFEPFRPVNTSLY+CDNKFHTEAL++LLED+ KFGFI++DGNG LF  L+GNT+  L KF+VDLPKKH RGGQSALRFARLRVEKR+NYVRKVAETA Q+FI+NDKVNVEG++LAGSADFK +LH S++FD+RLLAKV+KKVD+SYGG QG +QAI +S D ++N+KF+QEQKLI KFF+EI+QNTG+ C+G+DD + GL+MG +ET+I WENL + RY+L NN +GET E ILRPEQ K  ++F+DK +  +LE+++EQL+L++L  KY +YGA LE VSDKS EGSQFVKGFGGIGG+LRYKVDF  +  ++    ++DF +D Y+
Sbjct:    2 TDKNIEIWKMKKLISKLQSVRGNGTSMISIIIKPGDQIFRINKMLTDEAGTASNIKSRVNRLSVLSAITSAQQRLKLRNKIPTNGLVVYCGTIMMDDGKEKKFSIDFEPFRPVNTSLYMCDNKFHTEALASLLEDDMKFGFIIMDGNGVLFANLSGNTKVTLAKFSVDLPKKHSRGGQSALRFARLRVEKRNNYVRKVAETACQLFITNDKVNVEGIILAGSADFKTDLHGSNLFDMRLLAKVIKKVDISYGGNQGLHQAILLSMDCISNLKFVQEQKLISKFFEEINQNTGRSCYGIDDIVNGLKMGVIETIIAWENLPLIRYSLINNESGETCEKILRPEQSKIASNFIDKASKVKLEIVDEQLYLDYLAEKYNEYGAGLETVSDKSPEGSQFVKGFGGIGGLLRYKVDFVEI-AELDYDSQDDFYMDAYD 433

[Close]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Planmine SMEST
Match: SMESG000038950.1 (SMESG000038950.1)

HSP 1 Score: 557.755 bits (1436), Expect = 0.000e+0
Identity = 273/390 (70.00%), Postives = 334/390 (85.64%), Query Frame = 3

Query:  219 MLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCGTIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGFIVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKRHNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLAKVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNTGKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRPEQKKERAHFLDKTTNAXXXXXXXXXXXXXXXXKYKDYGAVLEIVSDKSAEGSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDYE 1388
            ML DE GTASNIKSRVNRLSVLSAITS QQ+LKL N++PTNGLVVYCGTIM DDGKEKK SIDFEPFRPVNTSLY+CDNKFHTEAL++LLED+ KFGFI++DGNG LF  L+GNT+  L KF+VDLPKKH RGGQSALRFARLRVEKR+NYVRKVAETA Q+FI+NDKVNVEG++LAGSADFK +LH S++FD+RLLAKV+KKVD+SYGG QG +QAI +S D ++N+KF+QEQKLI KFF+EI+QNTG+ C+G+DD + GL+MG +ET+I WENL + RY+L NN +GET E ILRPEQ K  ++F+DK +  +LE+++EQL+L++L  KY +YGA LE VSDKS EGSQFVKGFGGIGG+LRYKVDF  +  ++    ++DF +D Y+
Sbjct:    1 MLTDEAGTASNIKSRVNRLSVLSAITSAQQRLKLRNKIPTNGLVVYCGTIMMDDGKEKKFSIDFEPFRPVNTSLYMCDNKFHTEALASLLEDDMKFGFIIMDGNGVLFANLSGNTKVTLAKFSVDLPKKHSRGGQSALRFARLRVEKRNNYVRKVAETACQLFITNDKVNVEGIILAGSADFKTDLHGSNLFDMRLLAKVIKKVDISYGGNQGLHQAILLSMDCISNLKFVQEQKLISKFFEEINQNTGRSCYGIDDIVNGLKMGVIETIIAWENLPLIRYSLINNESGETCEKILRPEQSKIASNFIDKASKVKLEIVDEQLYLDYLAEKYNEYGAGLETVSDKSPEGSQFVKGFGGIGGLLRYKVDFVEI-AELDYDSQDDFYMDAYD 389

The following BLAST results are available for this feature:

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Human
BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Celegans
BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Fly
BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Zebrafish
BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Xenopus
BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Mouse
BLAST of Eukaryotic peptide chain release factor subunit 1 vs. UniProt/SwissProt
BLAST of Eukaryotic peptide chain release factor subunit 1 vs. TrEMBL
BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Cavefish
BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Sea Lamprey
BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Yeast
BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Nematostella
BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Medaka
BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Planmine SMEST

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ETF1	0.000e+0	73.62	eukaryotic translation termination factor 1 [Sourc...	[more]
ETF1	0.000e+0	73.66	eukaryotic translation termination factor 1 [Sourc...	[more]
ETF1	0.000e+0	73.40	eukaryotic translation termination factor 1 [Sourc...	[more]
ETF1	2.461e-129	84.62	eukaryotic translation termination factor 1 [Sourc...	[more]
ETF1	1.095e-67	83.19	eukaryotic translation termination factor 1 [Sourc...	[more]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3


Match Name	E-value	Identity	Description
erfa-1	0.000e+0	68.53	Eukaryotic peptide chain release factor subunit 1 ...	[more]
erfa-1	0.000e+0	68.53	Eukaryotic peptide chain release factor subunit 1 ...	[more]
erfa-1	0.000e+0	68.53	Eukaryotic peptide chain release factor subunit 1 ...	[more]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
eRF1	0.000e+0	71.79	gene:FBgn0036974 transcript:FBtr0078174	[more]
eRF1	0.000e+0	71.79	gene:FBgn0036974 transcript:FBtr0078178	[more]
eRF1	0.000e+0	71.79	gene:FBgn0036974 transcript:FBtr0078173	[more]
eRF1	0.000e+0	71.79	gene:FBgn0036974 transcript:FBtr0078175	[more]
eRF1	0.000e+0	71.79	gene:FBgn0036974 transcript:FBtr0078177	[more]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4


Match Name	E-value	Identity	Description
etf1b	0.000e+0	75.53	eukaryotic translation termination factor 1b [Sour...	[more]
etf1b	0.000e+0	75.53	eukaryotic translation termination factor 1b [Sour...	[more]
etf1b	0.000e+0	72.41	eukaryotic translation termination factor 1b [Sour...	[more]
etf1a	0.000e+0	69.18	eukaryotic translation termination factor 1a [Sour...	[more]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2


Match Name	E-value	Identity	Description
etf1	0.000e+0	73.85	eukaryotic translation termination factor 1 [Sourc...	[more]
etf1	0.000e+0	72.20	eukaryotic translation termination factor 1 [Sourc...	[more]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Etf1	0.000e+0	73.62	eukaryotic translation termination factor 1 [Sourc...	[more]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P35615\|ERF1_XENLA	0.000e+0	74.08	Eukaryotic peptide chain release factor subunit 1 ...	[more]
sp\|P62498\|ERF1_XENTR	0.000e+0	73.85	Eukaryotic peptide chain release factor subunit 1 ...	[more]
sp\|Q9GR88\|ERF1_POLMI	0.000e+0	74.46	Eukaryotic peptide chain release factor subunit 1 ...	[more]
sp\|Q5U2Q7\|ERF1_RAT	0.000e+0	73.62	Eukaryotic peptide chain release factor subunit 1 ...	[more]
sp\|P62497\|ERF1_RABIT	0.000e+0	73.62	Eukaryotic peptide chain release factor subunit 1 ...	[more]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1I8JEF3	0.000e+0	74.83	eRF1_1 domain-containing protein OS=Macrostomum li...	[more]
A0A1S3H0N4	0.000e+0	73.70	eukaryotic peptide chain release factor subunit 1 ...	[more]
V4AI12	0.000e+0	73.56	eRF1_1 domain-containing protein OS=Lottia gigante...	[more]
A0A0R3UQ50	0.000e+0	72.71	eRF1_1 domain-containing protein OS=Mesocestoides ...	[more]
S4RP18	0.000e+0	73.58	Eukaryotic translation termination factor 1a OS=Pe...	[more]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
etf1b	0.000e+0	75.12	eukaryotic peptide chain release factor subunit 1 ...	[more]
etf1a	0.000e+0	74.18	eukaryotic peptide chain release factor subunit 1-...	[more]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000006985.1	0.000e+0	73.58	pep scaffold:Pmarinus_7.0:GL476537:382840:396315:-...	[more]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
SUP45	0.000e+0	64.42	Polypeptide release factor (eRF1) in translation t...	[more]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO41505	0.000e+0	72.56	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
etf1a	0.000e+0	74.41	eukaryotic translation termination factor 1 [Sourc...	[more]

BLAST of Eukaryotic peptide chain release factor subunit 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3


Match Name	E-value	Identity	Description
SMESG000019227.1	0.000e+0	100.00	SMESG000019227.1	[more]
SMESG000038950.1	0.000e+0	69.52	SMESG000038950.1	[more]
SMESG000038950.1	0.000e+0	70.00	SMESG000038950.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30034032 ID=SMED30034032|Name=Eukaryotic peptide chain release factor subunit 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1546bp
TACTGTATTAACATATTTTTTTGCGTTTTGATCTTAGTATTACCGTATTA
TCTAAACATAAAATGTCTGCGAATACTAATGAATCTGGTTCGGATAATAA
TGTAGAAATGTGGAAAATAAAGAAGCTGATTAAAAATTTAGAAGCAGCAA
GAGGGAATGGAACCAGTATGATTTCATTGATTATTAAACCAGGGGATCAA
ATTAATAAAGTTGCTAAGATGTTAGCTGATGAATATGGCACAGCTTCTAA
CATTAAAAGTAGAGTCAATCGTTTGTCTGTGCTTAGTGCTATTACATCAA
CACAACAAAAGTTAAAATTATACAATAGAGTTCCAACTAATGGCTTAGTA
GTGTATTGTGGAACAATTATGACAGATGATGGAAAGGAAAAGAAAGTCAG
TATAGATTTTGAACCTTTTAGACCTGTCAACACTTCTTTGTATTTGTGTG
ATAATAAATTTCACACTGAAGCCTTATCAGCTTTATTGGAAGACAATAGC
AAATTTGGTTTTATTGTTGTAGATGGAAATGGTGCTCTTTTTGGAACCCT
TGCTGGTAATACAAGAGATATATTGCACAAATTTACTGTTGACCTGCCCA
AGAAACATGGACGAGGTGGTCAATCTGCATTACGTTTTGCTCGATTAAGA
GTTGAAAAGCGGCACAATTACGTTAGAAAAGTGGCAGAAACTGCAGTCCA
GATGTTTATTAGTAATGATAAGGTTAACGTGGAAGGTTTAGTTCTGGCTG
GAAGTGCTGATTTCAAAAATGAACTTCACGAATCTGATATATTTGATTTG
AGACTTTTAGCAAAAGTTTTGAAAAAAGTTGATGTTTCTTATGGCGGAGA
ACAAGGGTTTAATCAAGCAATTGAAATATCTGCAGACAGTCTGGCAAATG
TTAAATTTATTCAAGAGCAAAAATTGATAAAAAAGTTTTTTGATGAAATT
CATCAAAATACCGGCAAATTCTGCTTTGGGTTAGACGATACAATGAGAGG
TTTGGAAATGGGAGCTGTTGAAACCCTAATTGTATGGGAAAATTTAGCAG
TCTGTCGCTATACTTTACGAAATAATAGTACGGGTGAAACTACGGAGCTC
ATTCTTAGACCTGAACAGAAAAAAGAAAGAGCGCATTTTTTGGATAAAAC
TACCAATGCAGAATTAGAATTGTTGGAAGAACAACTATTTTTAGAATTTT
TGACTCTAAAATATAAAGATTATGGAGCCGTTTTGGAAATTGTGAGTGAC
AAATCAGCAGAAGGTTCCCAATTTGTTAAAGGATTTGGAGGAATTGGTGG
CATTTTAAGATATAAAGTGGATTTTCAAGGTATGGATACTGATATGTTTG
AACAAGAGGAAGAGGACTTTAATATCGATGATTACGAATGACTATTACTG
GGATCAGATGACACCTTAAGAACTCGCGAAGGTCTTCAAGAAGTCGGTGT
TCGTCTCGGTTAAATGTTACAATTAAGAAAACAATGTTATTGTTATGTGA
TGATTTATTAAATTATAATTAAATATTAATTTTTTCTTAAAAACAA

protein sequence of SMED30034032-orf-1

>SMED30034032-orf-1 ID=SMED30034032-orf-1|Name=SMED30034032-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=443bp
MSANTNESGSDNNVEMWKIKKLIKNLEAARGNGTSMISLIIKPGDQINKV
AKMLADEYGTASNIKSRVNRLSVLSAITSTQQKLKLYNRVPTNGLVVYCG
TIMTDDGKEKKVSIDFEPFRPVNTSLYLCDNKFHTEALSALLEDNSKFGF
IVVDGNGALFGTLAGNTRDILHKFTVDLPKKHGRGGQSALRFARLRVEKR
HNYVRKVAETAVQMFISNDKVNVEGLVLAGSADFKNELHESDIFDLRLLA
KVLKKVDVSYGGEQGFNQAIEISADSLANVKFIQEQKLIKKFFDEIHQNT
GKFCFGLDDTMRGLEMGAVETLIVWENLAVCRYTLRNNSTGETTELILRP
EQKKERAHFLDKTTNAELELLEEQLFLEFLTLKYKDYGAVLEIVSDKSAE
GSQFVKGFGGIGGILRYKVDFQGMDTDMFEQEEEDFNIDDYE*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0003747	translation release factor activity
GO:0008079	translation termination factor activity
GO:0016149	translation release factor activity, codon specific

Vocabulary: **biological process**
Term	Definition
GO:0006449	regulation of translational termination
GO:0000184	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
GO:0006412	translation
GO:0006415	translational termination

Vocabulary: **INTERPRO**
Term	Definition
IPR042226	eFR1_2_sf
IPR004403	Peptide_chain-rel_eRF1/aRF1
IPR005140	eRF1_1_Pelota
IPR005142	eRF1_3
IPR024049	Release_factor_eRF1/aRF1_N
IPR005141	eRF1_2
IPR029064	L30e-like

Vocabulary: **cellular component**
Term	Definition
GO:0005737	cytoplasm

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005140	eRF1 domain 1/Pelota-like	SMART	SM01194	eRF1_1_2	coord: 7..143 e-value: 2.2E-47 score: 173.4
IPR005140	eRF1 domain 1/Pelota-like	PFAM	PF03463	eRF1_1	coord: 20..140 e-value: 5.6E-20 score: 71.7
IPR005141	eRF1 domain 2	PFAM	PF03464	eRF1_2	coord: 147..278 e-value: 2.1E-44 score: 151.0
IPR029064	50S ribosomal protein L30e-like	GENE3D	G3DSA:3.30.1330.30		coord: 280..441 e-value: 2.4E-56 score: 192.0
IPR029064	50S ribosomal protein L30e-like	SUPERFAMILY	SSF55315	L30e-like	coord: 279..422
IPR005142	eRF1 domain 3	PFAM	PF03465	eRF1_3	coord: 282..419 e-value: 2.7E-34 score: 118.1
IPR042226	eRF1, domain 2 superfamily	GENE3D	G3DSA:3.30.420.60		coord: 139..278 e-value: 7.9E-63 score: 212.6
IPR004403	Peptide chain release factor eRF1/aRF1	TIGRFAM	TIGR03676	TIGR03676	coord: 15..419 e-value: 1.0E-129 score: 430.8
IPR004403	Peptide chain release factor eRF1/aRF1	PANTHER	PTHR10113	PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1	coord: 3..431
IPR024049	Peptide Chain Release Factor eRF1/aRF1, N-terminal	GENE3D	G3DSA:3.30.960.10		coord: 2..138 e-value: 2.3E-65 score: 220.5
IPR024049	Peptide Chain Release Factor eRF1/aRF1, N-terminal	SUPERFAMILY	SSF55481	N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1	coord: 10..144
None	No IPR available	SUPERFAMILY	SSF53137	Translational machinery components	coord: 146..277