Sperm surface protein Sp17

Overview


Name	Sperm surface protein Sp17
Smed ID	SMED30034016
Length (bp)	1425

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Sperm surface protein Sp17 (SMED30034016) t-SNE clustered cells

Violin plots show distribution of expression levels for Sperm surface protein Sp17 (SMED30034016) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Sperm surface protein Sp17 (SMED30034016) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Sperm surface protein Sp17 (SMED30034016) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30034016

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 33

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
intestinal phagocyte	SMED30034016	SMESG000046919.1	Contig367	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30034016	SMESG000046919.1	Contig367	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30034016	SMESG000062750.1	Contig367	newmark_ests	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30034016	SMESG000062750.1	Contig367	uc_Smed_v2	PMID:23079596 Forsthoefel et al., 2012
head region	SMED30034016	SMESG000046919.1	SmedASXL_015865	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
head region	SMED30034016	SMESG000046919.1	SmedASXL_015863	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
head region	SMED30034016		SmedASXL_074411	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30034016	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30034016	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30034016	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30034016	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30034016	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neuron	SMED30034016	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30034016	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchyma	SMED30034016	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30034016	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30034016	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30034016	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034016	SMESG000046919.1	Contig26910	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034016	SMESG000046919.1	Contig26910	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034016	SMESG000046919.1	Contig3808	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034016	SMESG000046919.1	Contig3808	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034016	SMESG000046919.1	Contig10753	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034016	SMESG000046919.1	Contig10753	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034016	SMESG000046919.1	Contig36377	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034016	SMESG000046919.1	Contig36377	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034016	SMESG000004166.1	Contig3808	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30034016	SMESG000004166.1	Contig3808	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
head region	SMED30034016	SMESG000046919.1	dd_Smed_v6_4783_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
reproductive organ	SMED30034016	SMESG000046919.1	Contig26910	newmark_ests	PMID:28434803 Rouhana et al., 2017
reproductive organ	SMED30034016	SMESG000046919.1	Contig26910	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
epidermis	SMED30034016	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
ciliated epithelial cell	SMED30034016	SMESG000046919.1	dd_Smed_v4_4783_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of Sperm surface protein Sp17 vs. Ensembl Human
Match: SPA17 (sperm autoantigenic protein 17 [Source:HGNC Symbol;Acc:HGNC:11210])

HSP 1 Score: 97.8265 bits (242), Expect = 1.486e-23
Identity = 63/136 (46.32%), Postives = 88/136 (64.71%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXX---XXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQK 457
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  G+K+EDRFYNNH+F+       +   +E+   + K +          E D +KEE AA+ IQA+FRGH  R+  K
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDP-AEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQISGKEEETSVTILDSSEEDKEKEEVAAVKIQAAFRGHIAREEAK 135

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Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXX---XXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQK 457
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  G+K+EDRFYNNH+F+       +   +E+   + K +          E D +KEE AA+ IQA+FRGH  R+  K
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDP-AEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQISGKEEETSVTILDSSEEDKEKEEVAAVKIQAAFRGHIAREEAK 135

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BLAST of Sperm surface protein Sp17 vs. Ensembl Human
Match: CABYR (calcium binding tyrosine phosphorylation regulated [Source:HGNC Symbol;Acc:HGNC:15569])

HSP 1 Score: 51.6026 bits (122), Expect = 4.566e-8
Identity = 25/51 (49.02%), Postives = 31/51 (60.78%), Query Frame = 2

Query:   74 SNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYD 226
            S  RL VP G + +LEG++R VL+  P NI  FAA YF+EL   R  T  D
Sbjct:    4 SKPRLVVPYGLKTLLEGISRAVLKTNPSNINQFAAAYFQELTMYRGNTTMD 54

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Query:   74 SNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYD 226
            S  RL VP G + +LEG++R VL+  P NI  FAA YF+EL   R  T  D
Sbjct:    4 SKPRLVVPYGLKTLLEGISRAVLKTNPSNINQFAAAYFQELTMYRGNTTMD 54

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Query:   74 SNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYD 226
            S  RL VP G + +LEG++R VL+  P NI  FAA YF+EL   R  T  D
Sbjct:    4 SKPRLVVPYGLKTLLEGISRAVLKTNPSNINQFAAAYFQELTMYRGNTTMD 54

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BLAST of Sperm surface protein Sp17 vs. Ensembl Celegans
Match: F39H12.3 (pep chromosome:WBcel235:X:838144:841493:-1 gene:WBGene00018214.1 transcript:F39H12.3.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F39H12.3)

HSP 1 Score: 65.4698 bits (158), Expect = 5.320e-12
Identity = 56/193 (29.02%), Postives = 93/193 (48.19%), Query Frame = 2

Query:   92 VPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQE--------TGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFER-------QNQKPNSNEEIDLNNSQINQEDEEID---IDLNDPEVEKAAVKIQSVYKGFRSRKTTERI 616
            VP   + +LE LAREVLR QP ++  F   +F+E L+ R+E          Y+   +  A L+ +F                          +E  R A+   +DT     AA  IQA+F+GH  R        + + +S+E++D  N++ +Q+   +    ID++ PE ++AA KIQS  +GF +RK  +++
Sbjct:    9 VPHDLRPILEALAREVLRSQPSDVAEFGHMFFDEYLKHRRENRNILKDPAAYE---VFRADLQKKF--------------------------AEVERPAS--PMDT-----AATKIQAAFKGHLVRAHPEKYGMSTRTSSSEKLDSANNKKDQKRHSVGGYTIDVDTPE-DRAATKIQSEIRGFLTRKHVDKM 164

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BLAST of Sperm surface protein Sp17 vs. Ensembl Fly
Match: igl (gene:FBgn0013467 transcript:FBtr0087362)

HSP 1 Score: 58.151 bits (139), Expect = 3.206e-10
Identity = 30/68 (44.12%), Postives = 48/68 (70.59%), Query Frame = 2

Query:  401 AAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQE-DEEIDIDLNDPEVEKAAVKIQSVYKGFRSRK 601
            AA+ IQ++FRGH  R+    ++ E+ D+   +I ++  EE+DIDL DPE+ KAA KIQ+ ++G ++RK
Sbjct:   52 AALKIQSTFRGHLARKLVNKDAPEDEDI--QEITKKVAEELDIDLTDPELNKAATKIQASFRGHKTRK 117

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BLAST of Sperm surface protein Sp17 vs. Ensembl Fly
Match: igl (gene:FBgn0013467 transcript:FBtr0332267)

HSP 1 Score: 58.151 bits (139), Expect = 3.206e-10
Identity = 30/68 (44.12%), Postives = 48/68 (70.59%), Query Frame = 2

Query:  401 AAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQE-DEEIDIDLNDPEVEKAAVKIQSVYKGFRSRK 601
            AA+ IQ++FRGH  R+    ++ E+ D+   +I ++  EE+DIDL DPE+ KAA KIQ+ ++G ++RK
Sbjct:   52 AALKIQSTFRGHLARKLVNKDAPEDEDI--QEITKKVAEELDIDLTDPELNKAATKIQASFRGHKTRK 117

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BLAST of Sperm surface protein Sp17 vs. Ensembl Fly
Match: igl (gene:FBgn0013467 transcript:FBtr0087361)

HSP 1 Score: 58.151 bits (139), Expect = 2.972e-9
Identity = 30/68 (44.12%), Postives = 48/68 (70.59%), Query Frame = 2

Query:  401 AAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQE-DEEIDIDLNDPEVEKAAVKIQSVYKGFRSRK 601
            AA+ IQ++FRGH  R+    ++ E+ D+   +I ++  EE+DIDL DPE+ KAA KIQ+ ++G ++RK
Sbjct:  170 AALKIQSTFRGHLARKLVNKDAPEDEDIQ--EITKKVAEELDIDLTDPELNKAATKIQASFRGHKTRK 235

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BLAST of Sperm surface protein Sp17 vs. Ensembl Zebrafish
Match: spa17 (sperm autoantigenic protein 17 [Source:ZFIN;Acc:ZDB-GENE-061027-337])

HSP 1 Score: 106.301 bits (264), Expect = 5.459e-24
Identity = 48/70 (68.57%), Postives = 57/70 (81.43%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFY 268
            MAVP+SNT LR+P+GF N+LEGL REVLR QPE+I +FAA YF ELL+ R+E+G  D A  GAKLEDRFY
Sbjct:    1 MAVPFSNTHLRIPRGFGNLLEGLTREVLREQPEDIATFAAVYFTELLKAREESGL-DPAEWGAKLEDRFY 69

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BLAST of Sperm surface protein Sp17 vs. Ensembl Xenopus
Match: mettl4 (methyltransferase like 4 [Source:Xenbase;Acc:XB-GENE-992320])

HSP 1 Score: 95.9005 bits (237), Expect = 1.257e-20
Identity = 59/148 (39.86%), Postives = 85/148 (57.43%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEE--------QQSAAVKIQSEYRRFAAKK-------EVDTKKEEEAAIVIQASFRGHFERQNQK 457
            M++P+SNT  R+P+GF N+LEGL REVLR QP++I  F A YF +LL+ RQ+T + D A  GA LEDRFYNN+ F++        E        ++S ++   S+    +          E D  ++ +AA  IQA+FRG+  R   K
Sbjct:    1 MSIPFSNTHYRIPRGFANLLEGLTREVLRHQPKDIPLFGAKYFSQLLQQRQDTDF-DPAQWGAALEDRFYNNYDFQHTELKKYTSEPSELKKSKRKSFSMLDNSDTYPLSPSTSLEQKEIETDDMRKMQAATTIQAAFRGYNVRHKVK 147

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BLAST of Sperm surface protein Sp17 vs. Ensembl Xenopus
Match: ENSXETT00000015907.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV460425.1:107652:107837:1 gene:ENSXETG00000009842.1 transcript:ENSXETT00000015907.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.0542 bits (105), Expect = 5.502e-6
Identity = 21/34 (61.76%), Postives = 27/34 (79.41%), Query Frame = 2

Query:  503 QEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRKT 604
            + +EEIDIDLN PE EKAA+ IQS ++ F+ RKT
Sbjct:   24 KAEEEIDIDLNAPETEKAALAIQSKFRRFQKRKT 57

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BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
Match: Spa17 (sperm autoantigenic protein 17 [Source:MGI Symbol;Acc:MGI:1333778])

HSP 1 Score: 95.9005 bits (237), Expect = 5.029e-23
Identity = 62/134 (46.27%), Postives = 90/134 (67.16%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQS-AAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQK 457
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  GAK+EDRFYNNH+FK        E++ + ++ + ++    F    E + ++EE AA+ IQ+ FRGH  R+  K
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFENLLEKREKTSFDP-AEWGAKVEDRFYNNHAFKEQEQVEKCEQELAKSSGREETPVTPFEESTEEEREQEEAAALKIQSLFRGHVAREEVK 133

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BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
Match: Spa17 (sperm autoantigenic protein 17 [Source:MGI Symbol;Acc:MGI:1333778])

HSP 1 Score: 93.9745 bits (232), Expect = 1.588e-22
Identity = 61/131 (46.56%), Postives = 89/131 (67.94%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQS-AAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQ 448
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  GAK+EDRFYNNH+FK        E++ + ++ + ++    F    E + ++EE AA+ IQ+ FRGH  R+
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFENLLEKREKTSFDP-AEWGAKVEDRFYNNHAFKEQEQVEKCEQELAKSSGREETPVTPFEESTEEEREQEEAAALKIQSLFRGHVARE 130

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BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
Match: Cabyr (calcium-binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2) [Source:MGI Symbol;Acc:MGI:1918382])

HSP 1 Score: 53.9138 bits (128), Expect = 5.432e-8
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 2

Query:   74 SNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDI 232
            S  RL VP G + +LEG++R +L+  P NI  FAA YF+EL+  R+     DI
Sbjct:    4 SKPRLVVPYGLKTLLEGVSRAILKTNPTNITQFAAVYFKELIVFREGNSSLDI 56

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BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
Match: Cabyr (calcium-binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2) [Source:MGI Symbol;Acc:MGI:1918382])

HSP 1 Score: 53.9138 bits (128), Expect = 9.086e-8
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 2

Query:   74 SNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDI 232
            S  RL VP G + +LEG++R +L+  P NI  FAA YF+EL+  R+     DI
Sbjct:    4 SKPRLVVPYGLKTLLEGVSRAILKTNPTNITQFAAVYFKELIVFREGNSSLDI 56

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BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
Match: Cabyr (calcium-binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2) [Source:MGI Symbol;Acc:MGI:1918382])

HSP 1 Score: 54.6842 bits (130), Expect = 1.706e-7
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 2

Query:   74 SNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDI 232
            S  RL VP G + +LEG++R +L+  P NI  FAA YF+EL+  R+     DI
Sbjct:    4 SKPRLVVPYGLKTLLEGVSRAILKTNPTNITQFAAVYFKELIVFREGNSSLDI 56

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BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
Match: sp|P36425|SP17_RABIT (Sperm surface protein Sp17 OS=Oryctolagus cuniculus OX=9986 GN=SPA17 PE=1 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 1.329e-28
Identity = 65/133 (48.87%), Postives = 90/133 (67.67%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQK 457
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  GAK++DRFYNNH+F+   +     E++S +V  + E        E D  KEE AA+ IQA+FRGH  R++ K
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFENLLEKREKTNFDP-AEWGAKVDDRFYNNHAFQEHESEKCEAEEKSQSV-TEEETPVLTIDSEDDKDKEEMAALKIQAAFRGHLAREDVK 131

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BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
Match: sp|O19021|SP17_MACFA (Sperm surface protein Sp17 OS=Macaca fascicularis OX=9541 GN=SPA17 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 5.401e-23
Identity = 63/143 (44.06%), Postives = 87/143 (60.84%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQ----------SAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQK 457
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  G+K+EDRFYNNH+F+       ++ +Q           A+V I         K+EV       AA+ IQA+FRGH  R+  K
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDP-AEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQIPGKEEEASVTILDSSEEDKEKEEV-------AAVKIQAAFRGHVAREEVK 135

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BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
Match: sp|Q15506|SP17_HUMAN (Sperm surface protein Sp17 OS=Homo sapiens OX=9606 GN=SPA17 PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 7.136e-23
Identity = 63/136 (46.32%), Postives = 88/136 (64.71%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXX---XXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQK 457
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  G+K+EDRFYNNH+F+       +   +E+   + K +          E D +KEE AA+ IQA+FRGH  R+  K
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDP-AEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQISGKEEETSVTILDSSEEDKEKEEVAAVKIQAAFRGHIAREEAK 135

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BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
Match: sp|Q95230|SP17_PAPHA (Sperm surface protein Sp17 OS=Papio hamadryas OX=9557 GN=SPA17 PE=2 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 1.063e-22
Identity = 66/154 (42.86%), Postives = 94/154 (61.04%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXX---XXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQED 511
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  G+K+EDRFYNNH+F+       +   +E+   + K +          E D +KEE AA+ IQA+FRGH  R+  K    + +     + N ED
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDP-AEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQVSGKEEETSVTILDSSEEDKEKEEVAAVKIQAAFRGHVAREEVKKMKTDSLQNEEKEENSED 153

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BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
Match: sp|Q62252|SP17_MOUSE (Sperm surface protein Sp17 OS=Mus musculus OX=10090 GN=Spa17 PE=1 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 3.517e-22
Identity = 62/134 (46.27%), Postives = 90/134 (67.16%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQS-AAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQK 457
            M++P+SNT  R+P+GF N+LEGL RE+LR QP+NI +FAA YFE LL  R++T +D  A  GAK+EDRFYNNH+FK        E++ + ++ + ++    F    E + ++EE AA+ IQ+ FRGH  R+  K
Sbjct:    1 MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFENLLEKREKTSFDP-AEWGAKVEDRFYNNHAFKEQEQVEKCEQELAKSSGREETPVTPFEESTEEEREQEEAAALKIQSLFRGHVAREEVK 133

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BLAST of Sperm surface protein Sp17 vs. TrEMBL
Match: K1PZL4 (Sperm surface protein Sp17 OS=Crassostrea gigas OX=29159 GN=CGI_10005609 PE=4 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 3.263e-56
Identity = 154/386 (39.90%), Postives = 230/386 (59.59%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQN----QKPNS----NEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHES-EDLPPAHGENQNYQEYDIEDSQTMLDESKSSLKENDMDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXX----XVEKAAIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFR 1177
            MAVP+SNT+LRVPKGFQN+LEGLARE LR QPENIY F A YF++L+++R++TG+D  A+ GA+LEDRFYNN SF++P+T+  + +QQ AA+KIQ+E+RR  A++EV+  KEE+AA+ IQ+ FRG  +RQ     + P +     EE D   S+     + +DIDL+DPEV +AAVKIQ+ +KG+++RK  +     A T+  +++ +           E E+ D     +     H   Q+ +E   +  + ML E   S  EN +    A     +   E K L + +  +  + ++       +VE+AA+KIQ+ FKG+++RK  E  +     +  E++     + ++V         DIDLNDPDV  AAVKIQ+   GFR
Sbjct:    1 MAVPFSNTKLRVPKGFQNILEGLARECLRSQPENIYEFGAKYFQQLIQVREQTGHDP-AVHGARLEDRFYNNDSFQSPSTDPGDPQQQEAALKIQTEFRRHHAEQEVEHMKEEDAAVKIQSGFRGFQDRQKVLEMKDPEAYAKQKEEADERLSKGGSSKDSVDIDLDDPEVAEAAVKIQAGFKGYKARKEVKDKKEKASTDSKSDSQLP---------SEKEMADAATKIQAGFRGHKTRQDMKEKKEKGKEDMLSEINKSELENAVVHIQAGFRGYQARKEMKHLMDSQPAKQPEEEVDIDLNDPDVEQAALKIQAGFKGYKTRKEFESKKM----ESSEVINAGKNEEEEV---------DIDLNDPDVANAAVKIQA---GFR 360

HSP 2 Score: 88.9669 bits (219), Expect = 4.416e-15
Identity = 109/372 (29.30%), Postives = 171/372 (45.97%), Query Frame = 2

Query:  326 AAVKIQSEYRRFAAKKEVDTKKEE------------------EAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQIN----------------------------------QEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDES-KSSLKENDMDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKAAIKIQSTFKGFRSRK------------TLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXX-----------------LNDPDVEKAAVKIQSVYKGFRNRKTIK 1195
            AAVKIQ+ ++ + A+KEV  KKE+                  +AA  IQA FRGH  RQ+ K    +  +   S+IN                                  Q +EE+DIDLNDP+VE+AA+KIQ+ +KG+++RK  E            +  +E + ++++  +E E +D               +  + D+ ++   +    ++ +K    DT  + VT  E +P    + E + +E+IDIDL DPEVEKAA+KIQ+ FKGFR  K            +  E +T  ++   E  E  L+     + +   + +                    LNDPDVE+AA+KIQ+ +KG++ RK IK
Sbjct:  173 AAVKIQAGFKGYKARKEVKDKKEKASTDSKSDSQLPSEKEMADAATKIQAGFRGHKTRQDMKEKKEKGKEDMLSEINKSELENAVVHIQAGFRGYQARKEMKHLMDSQPAKQPEEEVDIDLNDPDVEQAALKIQAGFKGYKTRKEFE------------SKKMESSEVINAGKNEEEEVDI--------------DLNDPDVANAAVKIQAGFRAHIKAKKSDTAKSSVTK-ESSP----VAEQESEEVIDIDLNDPEVEKAALKIQAGFKGFRKAKKATTQDQTDKTESKPEEETSSKAADKETEEAALKIQAGFRGYKTRQEVKEQMAKEEKKGEEQEEVDIDLNDPDVEQAALKIQAGFKGYQARKEIK 513

HSP 3 Score: 61.2326 bits (147), Expect = 3.284e-6
Identity = 45/117 (38.46%), Postives = 65/117 (55.56%), Query Frame = 2

Query:  314 EQQSAAVKIQSEYRRFAAKKEVDT---------------------KKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRK 601
            E + AA+KIQ+ ++ F   K+  T                     K+ EEAA+ IQA FRG+  RQ  K     E      +  +E EE+DIDLNDP+VE+AA+KIQ+ +KG+++RK
Sbjct:  399 EVEKAALKIQAGFKGFRKAKKATTQDQTDKTESKPEEETSSKAADKETEEAALKIQAGFRGYKTRQEVK-----EQMAKEEKKGEEQEEVDIDLNDPDVEQAALKIQAGFKGYQARK 510

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BLAST of Sperm surface protein Sp17 vs. TrEMBL
Match: A0A210PVC9 (Sperm surface protein Sp17 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT22352 PE=4 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 4.443e-52
Identity = 105/182 (57.69%), Postives = 140/182 (76.92%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEID-LNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRK 601
            MAVP+SNT+LRVPKGFQN+LEGLAREVLR QPENIY F A YF++LLR+R ETG+D  A+ GA++EDRFYNN +F++P  ++ + +QQ AA+KIQ+EYRR +AKKEVD  +EE+AA  IQA  RGH +RQ        ++D +   +   +  E+DI+L DPEVE AAVKIQ+ +KG+++RK
Sbjct:    1 MAVPFSNTKLRVPKGFQNILEGLAREVLRNQPENIYEFGAKYFDQLLRVRDETGHDP-AVHGARMEDRFYNNDAFRSPGVDSGDPQQQDAALKIQTEYRRHSAKKEVDDMREEDAATTIQAGVRGHQDRQ--------KVDEIKEGEARLKSAEVDINLEDPEVEAAAVKIQAGFKGYKARK 173

HSP 2 Score: 94.3597 bits (233), Expect = 1.630e-16
Identity = 100/282 (35.46%), Postives = 138/282 (48.94%), Query Frame = 2

Query:  395 EEAAIVIQASFRGHFERQNQKPN----------SNEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDAQTNQHNN----TDIEENTLVHSNID----EPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSSLKENDMDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKAAIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRK 1186
            E+AA+ IQ+SF+G   R+++  +          SN       S   ++ EEIDIDLNDPEVEKAAVKIQ+ +KG +SR+   +    + TN  NN    T   +   V   ID    +PE+                       I+ +   L   K    +    +P  P+ N   NP P        ++ IDIDL DPEV KAA+KIQSTFKG +SRK   +  T     ++E            +       +DIDLNDP+VEKAA+KIQS +KG ++RK
Sbjct:  419 EKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAK--KSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAA-----------------LKIQSTFKGLKSRKFGKNKTSQSSP--PINN--VNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPVPVPTEEE------VDIDLNDPEVEKAALKIQSTFKGLKSRK 671

HSP 3 Score: 89.7373 bits (221), Expect = 4.628e-15
Identity = 99/344 (28.78%), Postives = 157/344 (45.64%), Query Frame = 2

Query:  314 EQQSAAVKIQSEY-----RRFAAKKEVDTKKE----------------------------EEAAIVIQASFRG----HFERQNQKPNSNEEIDLNNSQIN-QEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDA--QTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSSLKENDMDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVE--KAAIKIQSTFKGFRSRKTLEEM--QTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXX------LNDPDVEKAAVKIQSVYKGFRNRKTIK 1195
            E + AA+KIQS +     R+F AKK   T  E                            E+AA+ IQA+F+G     F  +   P+   +I      +N   +EEIDIDLNDPEVEKAAVKIQ+ +KG +SRK  +  P     Q    N+ D + + +   +ID                        + D+E +   +  S   L+        A  T +++   PK  ++  E +  +ID+   + E  +AA+KIQ+ F+G+++RK ++E     GD+++  +  EE     +       +     +      LNDP+V KAA KIQ+ +KG++ RK +K
Sbjct:  652 EVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDID----------------------LTDPDVEKAAVKIQASFKGLR--------ARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 965

HSP 4 Score: 82.4185 bits (202), Expect = 8.850e-13
Identity = 108/327 (33.03%), Postives = 160/327 (48.93%), Query Frame = 2

Query:  326 AAVKIQSEYRRFAAKKEVDTKKE--------------------------EEAAIVIQASFRGHFERQNQKPN-SNEEIDLNNSQINQEDEE---------IDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDAQTNQHNNTDIE----ENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSSLKENDMDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKAAIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRK 1186
            AA KIQ+ ++ +  +KE++ K+                            +AA  IQA+F+GH  R+  + N S  E D  ++ I+  D++         IDIDL+DPEVEKAA KIQ+ YKG ++R+  + I     T+      +E    E  ++  ++ +PE+           A     +++      S+T    S S   E    TP  P++   E  E            IDIDL DPEVEKAA+KIQ+TFKG +SR+     +    + +L  ++      DKV     EE IDIDLNDP+V KAA+KIQS +KG ++RK
Sbjct:  253 AATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKA--------AVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQE-----------EIDIDLNDPEVEKAAVKIQATFKGLKSRRF--GAKKSSPTNNLNNMKPVTPPVDKVPV---EEEIDIDLNDPEVAKAALKIQSTFKGLKSRK 555

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BLAST of Sperm surface protein Sp17 vs. TrEMBL
Match: A0A267EAR1 (RIIa domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig011073g1 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 1.822e-47
Identity = 98/181 (54.14%), Postives = 134/181 (74.03%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRK 601
            M+VP+S+T+LRVP+GFQ++LEGLAREVLR QP +IY+F A YFEEL+R R+ETG DD A L AK+EDRFYNN SF+ P  + ++ +QQ A+  IQ+ +RR AA+KE    +E++AA  IQ++ RGH ER+  K ++ E+            EE+DIDLNDPEVE AA KIQ+ +KG+++RK
Sbjct:    3 MSVPFSSTKLRVPRGFQSLLEGLAREVLRSQPADIYTFGAMYFEELIRRREETGDDDPAQLAAKVEDRFYNNTSFQQPTIDPSDPQQQDASKVIQTNFRRHAAQKEAAKLREDKAASEIQSAVRGHQEREAIKQSAPEQGSA---------EEVDIDLNDPEVEAAAAKIQAGFKGYKTRK 174

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BLAST of Sperm surface protein Sp17 vs. TrEMBL
Match: A0A267EEY9 (RIIa domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig023834g1 PE=4 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 4.965e-47
Identity = 98/181 (54.14%), Postives = 134/181 (74.03%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRK 601
            M+VP+S+T+LRVP+GFQ++LEGLAREVLR QP +IY+F A YFEEL+R R+ETG DD A L AK+EDRFYNN SF+ P  + ++ +QQ A+  IQ+ +RR AA+KE    +E++AA  IQ++ RGH ER+  K ++ E+            EE+DIDLNDPEVE AA KIQ+ +KG+++RK
Sbjct:    3 MSVPFSSTKLRVPRGFQSLLEGLAREVLRSQPADIYTFGAMYFEELIRRREETGDDDPAQLAAKVEDRFYNNTSFQQPTIDPSDPQQQDASKVIQTNFRRHAAQKEAAKLREDKAASEIQSAVRGHQEREAIKQSAPEQGSA---------EEVDIDLNDPEVEAAAAKIQAGFKGYKTRK 174

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BLAST of Sperm surface protein Sp17 vs. TrEMBL
Match: A0A1I8GWL3 (RIIa domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 3.931e-46
Identity = 98/181 (54.14%), Postives = 134/181 (74.03%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRK 601
            M+VP+S+T+LRVP+GFQ++LEGLAREVLR QP +IY+F A YFEEL+R R+ETG DD A L AK+EDRFYNN SF+ P  + ++ +QQ A+  IQ+ +RR AA+KE    +E++AA  IQ++ RGH ER+  K ++ E+            EE+DIDLNDPEVE AA KIQ+ +KG+++RK
Sbjct:    1 MSVPFSSTKLRVPRGFQSLLEGLAREVLRSQPADIYTFGAMYFEELIRRREETGDDDPAQLAAKVEDRFYNNTSFQQPTIDPSDPQQQDASKVIQTNFRRHAAQKEAAKLREDKAASEIQSAVRGHQEREAIKQSAPEQGSA---------EEVDIDLNDPEVEAAAAKIQAGFKGYKTRK 172

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BLAST of Sperm surface protein Sp17 vs. Ensembl Cavefish
Match: ENSAMXT00000046795.1 (pep primary_assembly:Astyanax_mexicanus-2.0:2:22320681:22325023:1 gene:ENSAMXG00000038900.1 transcript:ENSAMXT00000046795.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 98.5969 bits (244), Expect = 1.279e-23
Identity = 46/70 (65.71%), Postives = 55/70 (78.57%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFY 268
            M+VP+SNT LRVP GF N+LEGLAREVLR QP++I +FAA YF  LL+ R E+G D  A  GA+LEDRFY
Sbjct:    1 MSVPFSNTNLRVPHGFGNILEGLAREVLREQPDDIPTFAAVYFTALLKQRDESGLDP-AEWGARLEDRFY 69

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BLAST of Sperm surface protein Sp17 vs. Ensembl Nematostella
Match: EDO46037 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR24])

HSP 1 Score: 102.834 bits (255), Expect = 4.941e-26
Identity = 62/138 (44.93%), Postives = 85/138 (61.59%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKK-----EEEAAIVIQASFRGHFERQNQK 457
            MAV Y+ T+LRVP GFQN+LEGLAREVLR QP++I  FAA YF+  L +R+ETG DD     AK  +        +  + + ++ E Q AA KIQ+ +R    ++++   K     EE AA+ IQ+SFRGH  R+  K
Sbjct:    1 MAVQYAPTKLRVPPGFQNLLEGLAREVLREQPDDIVQFAAQYFKNQLSIREETGKDD-----AKRGENLEKLMKGEEVDIDIDDPEVQKAATKIQASFRGHKTREQIKQDKQQKDEEEAAALKIQSSFRGHKAREEVK 133

HSP 2 Score: 45.4394 bits (106), Expect = 6.517e-6
Identity = 23/40 (57.50%), Postives = 29/40 (72.50%), Query Frame = 2

Query:  512 EEIDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDAQ 631
            EE+DID++DPEV+KAA KIQ+    FR  KT E+I  D Q
Sbjct:   71 EEVDIDIDDPEVQKAATKIQA---SFRGHKTREQIKQDKQ 107

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BLAST of Sperm surface protein Sp17 vs. Ensembl Nematostella
Match: EDO35043 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMM3])

HSP 1 Score: 73.559 bits (179), Expect = 1.043e-15
Identity = 58/173 (33.53%), Postives = 81/173 (46.82%), Query Frame = 2

Query:   83 RLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRK 601
            +L VP GF+N+LE L  E+LR +P+NI SFAAD+F E L  RQ  GYDD  LLG+++ D FY            N E  +    K +S             KK +E          GH+E    K N   +    N+   +  ++   + +    E AA KIQ+ Y G+  RK
Sbjct:    7 KLSVPSGFRNLLESLTVEILRVRPDNIPSFAADFFRERLAARQRNGYDD-CLLGSQI-DGFY------------NFEPDRPGNFKDKS-------------KKVKE---------HGHYEPARAKLNDTAD---GNNNCGESKKQTTSEDDGERCETAATKIQAGYHGYTCRK 140

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BLAST of Sperm surface protein Sp17 vs. Ensembl Medaka
Match: ENSORLT00000038630.1 (sperm autoantigenic protein 17 [Source:NCBI gene;Acc:105355434])

HSP 1 Score: 83.9593 bits (206), Expect = 7.572e-17
Identity = 39/70 (55.71%), Postives = 49/70 (70.00%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFY 268
            M+  +    LRVP+G   V+EGLAREVLR QPENI  +AA+YF  LLR R+E+G  D A   AKLEDR++
Sbjct:    1 MSAHFFTAHLRVPRGLGAVIEGLAREVLRDQPENIPEYAANYFNGLLRQREESGV-DPAAWAAKLEDRYH 69

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BLAST of Sperm surface protein Sp17 vs. Ensembl Medaka
Match: ENSORLT00000038228.1 (sperm autoantigenic protein 17 [Source:NCBI gene;Acc:105355434])

HSP 1 Score: 83.9593 bits (206), Expect = 8.044e-17
Identity = 39/70 (55.71%), Postives = 49/70 (70.00%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFY 268
            M+  +    LRVP+G   V+EGLAREVLR QPENI  +AA+YF  LLR R+E+G  D A   AKLEDR++
Sbjct:    1 MSAHFFTAHLRVPRGLGAVIEGLAREVLRDQPENIPEYAANYFNGLLRQREESGV-DPAAWAAKLEDRYH 69

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BLAST of Sperm surface protein Sp17 vs. Planmine SMEST
Match: SMESG000046919.1 (SMESG000046919.1)

HSP 1 Score: 540.806 bits (1392), Expect = 0.000e+0
Identity = 291/335 (86.87%), Postives = 291/335 (86.87%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSSLKENDMDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKAAIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLE 1063
            MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDS TMLDESKSSLKENDMDTPIAPVTNDEENP                                           EEMQTGDESQHLEIVEENLE
Sbjct:    1 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSHTMLDESKSSLKENDMDTPIAPVTNDEENP-------------------------------------------EEMQTGDESQHLEIVEENLE 292

HSP 2 Score: 57.3806 bits (137), Expect = 1.691e-8
Identity = 38/82 (46.34%), Postives = 54/82 (65.85%), Query Frame = 2

Query:  944 EKAAIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFXXXXXXXXXLNDPDVEKAAVKIQSVYKGFRNRKT 1189
            E+AAI IQ++F+G   R+     Q  + ++ +++    + + D+         IDIDLNDP+VEKAAVKIQSVYKGFR+RKT
Sbjct:  113 EEAAIVIQASFRGHFERQN----QKPNSNEEIDLNNSQINQEDE--------EIDIDLNDPEVEKAAVKIQSVYKGFRSRKT 182

[Close]

BLAST of Sperm surface protein Sp17 vs. Planmine SMEST
Match: SMESG000046919.1 (SMESG000046919.1)

HSP 1 Score: 436.032 bits (1120), Expect = 2.012e-148
Identity = 248/335 (74.03%), Postives = 248/335 (74.03%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSSLKENDMDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKAAIKIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLE 1063
            MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEID                                           RIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDS TMLDESKSSLKENDMDTPIAPVTNDEENP                                           EEMQTGDESQHLEIVEENLE
Sbjct:    1 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEID-------------------------------------------RIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSHTMLDESKSSLKENDMDTPIAPVTNDEENP-------------------------------------------EEMQTGDESQHLEIVEENLE 249

[Close]

BLAST of Sperm surface protein Sp17 vs. Planmine SMEST
Match: SMESG000046919.1 (SMESG000046919.1)

HSP 1 Score: 435.647 bits (1119), Expect = 1.330e-147
Identity = 262/350 (74.86%), Postives = 269/350 (76.86%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSSLKENDMDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXVEKAAIKIQSTFKGFRSRKT---------------LEEMQTGDESQHLEIVEENLE 1063
            MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEID                                           RIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDS TMLDESKSSLKENDM              +P+ LQ    +EIIDIDLTDP+VEKAA+KIQS FKGF++RK                 EEMQTGDESQHLEIVEENLE
Sbjct:    1 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEID-------------------------------------------RIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSHTMLDESKSSLKENDM--------------KPEELQ--NSEEIIDIDLTDPDVEKAAVKIQSAFKGFKTRKANEDTPIAPVTNDEENPEEMQTGDESQHLEIVEENLE 291

[Close]

BLAST of Sperm surface protein Sp17 vs. Planmine SMEST
Match: SMESG000046919.1 (SMESG000046919.1)

HSP 1 Score: 431.795 bits (1109), Expect = 2.651e-146
Identity = 244/334 (73.05%), Postives = 253/334 (75.75%), Query Frame = 2

Query:   59 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYXXXXXXXXXXXXXXEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQEDEEIDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESKSSLKENDMDTPIAPVTNDEENPEPKRLQXXXXXXXXXXXXXXXXV---------------------EKAAIKIQSTFKGFRSRK 997
            MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEID                                           RIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDS TMLDESKSSLKENDMDTPIAPVTNDEENPE  +  +  +   I  +  +  V                     EKAA+KIQS +KG+R+RK
Sbjct:    1 MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMRQETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRRFAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEID-------------------------------------------RIPSDAQTNQHNNTDIEENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSHTMLDESKSSLKENDMDTPIAPVTNDEENPEEMQTGDESQHLEIVEENLEGPVAGGHGDYVETHEEIHLYDETSEKAAVKIQSVYKGYRTRK 291

The following BLAST results are available for this feature:

BLAST of Sperm surface protein Sp17 vs. Ensembl Human
BLAST of Sperm surface protein Sp17 vs. Ensembl Celegans
BLAST of Sperm surface protein Sp17 vs. Ensembl Fly
BLAST of Sperm surface protein Sp17 vs. Ensembl Zebrafish
BLAST of Sperm surface protein Sp17 vs. Ensembl Xenopus
BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
BLAST of Sperm surface protein Sp17 vs. TrEMBL
BLAST of Sperm surface protein Sp17 vs. Ensembl Cavefish
BLAST of Sperm surface protein Sp17 vs. Ensembl Sea Lamprey
BLAST of Sperm surface protein Sp17 vs. Ensembl Yeast
BLAST of Sperm surface protein Sp17 vs. Ensembl Nematostella
BLAST of Sperm surface protein Sp17 vs. Ensembl Medaka
BLAST of Sperm surface protein Sp17 vs. Planmine SMEST

BLAST of Sperm surface protein Sp17 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SPA17	1.486e-23	46.32	sperm autoantigenic protein 17 [Source:HGNC Symbol...	[more]
SPA17	1.486e-23	46.32	sperm autoantigenic protein 17 [Source:HGNC Symbol...	[more]
CABYR	4.566e-8	49.02	calcium binding tyrosine phosphorylation regulated...	[more]
CABYR	4.566e-8	49.02	calcium binding tyrosine phosphorylation regulated...	[more]
CABYR	4.566e-8	49.02	calcium binding tyrosine phosphorylation regulated...	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
F39H12.3	5.320e-12	29.02	pep chromosome:WBcel235:X:838144:841493:-1 gene:WB...	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3


Match Name	E-value	Identity	Description
igl	3.206e-10	44.12	gene:FBgn0013467 transcript:FBtr0087362	[more]
igl	3.206e-10	44.12	gene:FBgn0013467 transcript:FBtr0332267	[more]
igl	2.972e-9	44.12	gene:FBgn0013467 transcript:FBtr0087361	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
spa17	5.459e-24	68.57	sperm autoantigenic protein 17 [Source:ZFIN;Acc:ZD...	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2


Match Name	E-value	Identity	Description
mettl4	1.257e-20	39.86	methyltransferase like 4 [Source:Xenbase;Acc:XB-GE...	[more]
ENSXETT00000015907.1	5.502e-6	61.76	pep primary_assembly:Xenopus_tropicalis_v9.1:KV460...	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Spa17	5.029e-23	46.27	sperm autoantigenic protein 17 [Source:MGI Symbol;...	[more]
Spa17	1.588e-22	46.56	sperm autoantigenic protein 17 [Source:MGI Symbol;...	[more]
Cabyr	5.432e-8	45.28	calcium-binding tyrosine-(Y)-phosphorylation regul...	[more]
Cabyr	9.086e-8	45.28	calcium-binding tyrosine-(Y)-phosphorylation regul...	[more]
Cabyr	1.706e-7	45.28	calcium-binding tyrosine-(Y)-phosphorylation regul...	[more]

BLAST of Sperm surface protein Sp17 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P36425\|SP17_RABIT	1.329e-28	48.87	Sperm surface protein Sp17 OS=Oryctolagus cuniculu...	[more]
sp\|O19021\|SP17_MACFA	5.401e-23	44.06	Sperm surface protein Sp17 OS=Macaca fascicularis ...	[more]
sp\|Q15506\|SP17_HUMAN	7.136e-23	46.32	Sperm surface protein Sp17 OS=Homo sapiens OX=9606...	[more]
sp\|Q95230\|SP17_PAPHA	1.063e-22	42.86	Sperm surface protein Sp17 OS=Papio hamadryas OX=9...	[more]
sp\|Q62252\|SP17_MOUSE	3.517e-22	46.27	Sperm surface protein Sp17 OS=Mus musculus OX=1009...	[more]

BLAST of Sperm surface protein Sp17 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
K1PZL4	3.263e-56	39.90	Sperm surface protein Sp17 OS=Crassostrea gigas OX...	[more]
A0A210PVC9	4.443e-52	57.69	Sperm surface protein Sp17 OS=Mizuhopecten yessoen...	[more]
A0A267EAR1	1.822e-47	54.14	RIIa domain-containing protein (Fragment) OS=Macro...	[more]
A0A267EEY9	4.965e-47	54.14	RIIa domain-containing protein (Fragment) OS=Macro...	[more]
A0A1I8GWL3	3.931e-46	54.14	RIIa domain-containing protein OS=Macrostomum lign...	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSAMXT00000046795.1	1.279e-23	65.71	pep primary_assembly:Astyanax_mexicanus-2.0:2:2232...	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Sperm surface protein Sp17 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Sperm surface protein Sp17 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO46037	4.941e-26	44.93	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35043	1.043e-15	33.53	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Sperm surface protein Sp17 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ENSORLT00000038630.1	7.572e-17	55.71	sperm autoantigenic protein 17 [Source:NCBI gene;A...	[more]
ENSORLT00000038228.1	8.044e-17	55.71	sperm autoantigenic protein 17 [Source:NCBI gene;A...	[more]

BLAST of Sperm surface protein Sp17 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4


Match Name	E-value	Identity	Description
SMESG000046919.1	1.691e-8	86.87	SMESG000046919.1	[more]
SMESG000046919.1	2.012e-148	74.03	SMESG000046919.1	[more]
SMESG000046919.1	1.330e-147	74.86	SMESG000046919.1	[more]
SMESG000046919.1	2.651e-146	73.05	SMESG000046919.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30034016 ID=SMED30034016|Name=Sperm surface protein Sp17|organism=Schmidtea mediterranea sexual|type=transcript|length=1425bp
CGTAAATTAATATCGAAAATTTTGTTGATTACTTATTAAAAGATTTTTAT
AATTAAATATGGCAGTTCCATATTCAAACACAAGATTAAGAGTGCCAAAA
GGTTTTCAAAATGTGTTAGAAGGTCTTGCACGTGAAGTCTTAAGATGCCA
ACCAGAAAATATTTATAGTTTTGCTGCTGATTATTTTGAAGAGTTATTAA
GAATGCGTCAAGAAACTGGTTATGACGATATAGCACTGTTAGGAGCGAAA
TTAGAAGACAGATTTTATAATAATCATAGTTTTAAAAATCCTAATACAAA
TACAAATAATGAAGAACAACAATCGGCTGCTGTTAAAATTCAATCGGAAT
ACCGTCGATTTGCCGCGAAAAAAGAAGTCGATACGAAGAAAGAAGAAGAG
GCTGCAATCGTTATACAAGCTTCATTCAGAGGGCATTTTGAAAGGCAGAA
CCAAAAACCAAATTCAAATGAAGAAATTGATTTAAATAATTCCCAAATTA
ATCAAGAAGATGAGGAAATTGACATCGACCTCAATGATCCGGAAGTCGAG
AAAGCCGCTGTTAAAATCCAGTCCGTTTATAAAGGTTTTCGAAGCCGTAA
AACGACAGAGAGAATTCCTTCCGATGCTCAAACTAATCAGCACAATAATA
CAGATATAGAAGAAAATACATTGGTACATTCAAACATTGATGAGCCAGAA
ATTATGGATAAACATGAATCTGAAGATCTTCCTCCGGCACATGGTGAAAA
TCAAAATTATCAAGAATATGATATAGAAGACTCTCAAACTATGCTCGACG
AATCTAAATCAAGTCTCAAAGAAAACGACATGGATACTCCAATAGCTCCG
GTAACTAACGATGAAGAAAATCCTGAGCCTAAAAGACTTCAAGAAATAAA
AGAAGATGAAATAATTGATATAGATTTAACCGATCCAGAGGTTGAGAAGG
CAGCAATAAAAATACAGTCGACATTTAAAGGTTTTCGAAGTCGAAAGACA
TTAGAGGAAATGCAAACTGGAGATGAATCTCAACATCTTGAAATAGTCGA
GGAAAATCTTGAAAAAAGTGATAAAGTTAAAGCATTCATCGATGAAGAGA
TCATTGATATAGATCTGAACGATCCTGACGTAGAGAAAGCAGCTGTCAAA
ATACAATCGGTTTATAAAGGTTTTCGAAATCGTAAAACTATTAAGGTTTG
AGCAGTAAAAGTATCATAGTATGTTATGCATCCGATCTGTGGGAGCACAA
GTGAAATTTGATTGATGTTTGTCTTAGATGTTAGAGATAAATTTTTATGA
TTATATATATAATTTCACCTTTTTTTACTTTATATTCTGAAATAATATAT
TTATCAATAGTGTAATATAAAAGTGCATGAGTAATTATTTTTTATTGAAT
TTTATTCTTTTTCATATCTTTCTGC

protein sequence of SMED30034016-orf-1

>SMED30034016-orf-1 ID=SMED30034016-orf-1|Name=SMED30034016-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=381bp
MAVPYSNTRLRVPKGFQNVLEGLAREVLRCQPENIYSFAADYFEELLRMR
QETGYDDIALLGAKLEDRFYNNHSFKNPNTNTNNEEQQSAAVKIQSEYRR
FAAKKEVDTKKEEEAAIVIQASFRGHFERQNQKPNSNEEIDLNNSQINQE
DEEIDIDLNDPEVEKAAVKIQSVYKGFRSRKTTERIPSDAQTNQHNNTDI
EENTLVHSNIDEPEIMDKHESEDLPPAHGENQNYQEYDIEDSQTMLDESK
SSLKENDMDTPIAPVTNDEENPEPKRLQEIKEDEIIDIDLTDPEVEKAAI
KIQSTFKGFRSRKTLEEMQTGDESQHLEIVEENLEKSDKVKAFIDEEIID
IDLNDPDVEKAAVKIQSVYKGFRNRKTIKV*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000020	protonephridia
PLANA:0000034	epidermis
PLANA:0000070	intestinal phagocyte
PLANA:0000099	neuron
PLANA:0000106	ciliated epithelial cell
PLANA:0000418	head
PLANA:0002032	epidermal cell
PLANA:0002089	reproductive organ

Vocabulary: **INTERPRO**
Term	Definition
IPR000048	IQ_motif_EF-hand-BS
IPR003117	cAMP_dep_PK_reg_su_I/II_a/b

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding

Vocabulary: **biological process**
Term	Definition
GO:0007339	binding of sperm to zona pellucida

Vocabulary: **cellular component**
Term	Definition
GO:0016020	membrane

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003117	cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain	SMART	SM00394	akam_3	coord: 14..51 e-value: 4.1E-13 score: 59.6
IPR003117	cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain	PFAM	PF02197	RIIa	coord: 14..50 e-value: 4.0E-14 score: 52.0
IPR000048	IQ motif, EF-hand binding site	SMART	SM00015	iq_5	coord: 357..379 e-value: 0.12 score: 21.5 coord: 294..316 e-value: 0.027 score: 23.6 coord: 111..133 e-value: 3.3E-4 score: 30.0 coord: 162..184 e-value: 0.25 score: 20.4 coord: 86..108 e-value: 0.9 score: 18.6
IPR000048	IQ motif, EF-hand binding site	PFAM	PF00612	IQ	coord: 164..181 e-value: 5.5E-4 score: 19.5 coord: 359..377 e-value: 0.0011 score: 18.5 coord: 89..106 e-value: 0.035 score: 13.9 coord: 296..314 e-value: 4.5E-4 score: 19.8 coord: 114..131 e-value: 0.0021 score: 17.7
IPR000048	IQ motif, EF-hand binding site	PROSITE	PS50096	IQ	coord: 164..192 score: 8.297
IPR000048	IQ motif, EF-hand binding site	PROSITE	PS50096	IQ	coord: 296..324 score: 9.359
IPR000048	IQ motif, EF-hand binding site	PROSITE	PS50096	IQ	coord: 359..380 score: 8.773
IPR000048	IQ motif, EF-hand binding site	PROSITE	PS50096	IQ	coord: 87..116 score: 7.821
IPR000048	IQ motif, EF-hand binding site	PROSITE	PS50096	IQ	coord: 112..141 score: 9.304
None	No IPR available	GENE3D	G3DSA:1.20.890.10		coord: 8..51 e-value: 3.2E-14 score: 54.0
None	No IPR available	GENE3D	G3DSA:1.20.5.190		coord: 294..378 e-value: 3.0E-9 score: 38.5 coord: 87..131 e-value: 1.5E-12 score: 49.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 186..204
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 177..204
None	No IPR available	PANTHER	PTHR10699	NEUROMODULIN	coord: 337..379 coord: 266..323 coord: 108..222 coord: 1..93
None	No IPR available	CDD	cd12100	DD_CABYR_SP17	coord: 12..50 e-value: 5.06886E-19 score: 77.6609
None	No IPR available	SUPERFAMILY	SSF47391	Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit	coord: 8..50

Main menu

Sperm surface protein Sp17

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 33

Owlery

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