Steroid 17-alpha-hydroxylase/17,20 lyase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30033976 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Homology
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Human
Match: CYP2J2 (cytochrome P450 family 2 subfamily J member 2 [Source:HGNC Symbol;Acc:HGNC:2634]) HSP 1 Score: 124.02 bits (310), Expect = 9.529e-30 Identity = 111/458 (24.24%), Postives = 198/458 (43.23%), Query Frame = 3
Query: 114 PPGPNRNSDEADFIINEKLIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSD-----YGPLWKTLQRNLHKGLRHCA-NLDRLNEIMYKCFDKFKETVEYRRGSK-NGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQY-----AEECDFPNDKP--------SKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKH---SLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNY 1418
PPGP R +F + + VQ ++ KYG + +L V I + E + ++G + ++ R +I + G WK +R LR+ L E + + E ++ G + +++N + N+I + F E F D +Q ++++ + ++ C N P ++ F + +KL+ + +I D+HRK D+ A + DF+ + +L SK G + H ++ S +D+ FA E + W +ALYP IQE ++ EI++ T + MPYTNAV+ E+QR + D + Y +PK ++I+ NL A+H D +W PD +P +FL E+G+ FK+R+ F+P+ +G R C+G + LF+ ++ +
Sbjct: 44 PPGPWRLPFLGNFFLVDFEQSHLEVQLFVKKYGNLFSLELGDISAVLITGLPLIKEALIHM--------DQNFGNRPVTPMREHIFKKNGLIMSSGQAWKEQRRFTLTALRNFGLGKKSLEERIQEEAQHLTEAIKEENGQPFDPHFKINNAV----SNIICSITFGERFEYQD-SWFQQLLKLLDEVTYLEASKTCQLYNVFPWIMKFLPGPHQTLFSNWKKLKLFVSHMI-------DKHRK--DWNPA--ETRDFI-----DAYLKEMSKHTGNPTSSFHEENLICSTLDLFFAGTETTSTTLRWALLYMALYPEIQEKVQAEIDRVIGQGQQPSTAA-RESMPYTNAVIHEVQRMGNIIPLNVPREVTVDTTLAGYHLPKGTMILTNLTALHRDPTEWATPDTFNPDHFL-ENGQ-FKKREAFMPFSIGKRACLGEQLARTELFIFFTSLMQKF 469
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Human
Match: CYP2R1 (cytochrome P450 family 2 subfamily R member 1 [Source:HGNC Symbol;Acc:HGNC:20580]) HSP 1 Score: 115.931 bits (289), Expect = 1.777e-28 Identity = 57/173 (32.95%), Postives = 93/173 (53.76%), Query Frame = 3
Query: 912 SLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHW--KSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKM 1424
S+ ++I A E N W +ALYPNIQ ++ EI+ N W K KMPYT AVL E+ R+ + FH+T +D + Y IPK + ++ NLY++H D+K W++P++ P+ FL G F +++ VP+ LG R C+G + +F+ ++ + +
Sbjct: 70 SVGELIIAGTETTTNVLRWAILFMALYPNIQGQVQKEIDLIMGPNGKP---SWDDKCKMPYTEAVLHEVLRFCNIVPLGIFHATSEDAVVRGYSIPKGTTVITNLYSVHFDEKYWRDPEVFHPERFLDSSG-YFAKKEALVPFSLGRRHCLGEHLARMEMFLFFTALLQRFHL 238
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Human
Match: CYP2U1 (cytochrome P450 family 2 subfamily U member 1 [Source:HGNC Symbol;Acc:HGNC:20582]) HSP 1 Score: 116.701 bits (291), Expect = 3.742e-27 Identity = 104/465 (22.37%), Postives = 215/465 (46.24%), Query Frame = 3
Query: 156 INEKLIYKKIVQKYIPK-YGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFN--DVDYKKYQG----AIEVIPQ----YAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEPFNIQFIR 1517
I+ +I +++ ++ + YG I + + VV ++D + E V++ ++ S R I++ + ++ + YGP+W+ ++ H LRH L +L+ + K ++FK + + + N+I L F + F+ + ++KK G +E+ C + P F ++L+++ + L+ I +H++ D + D D LL +EE + + ++ + + D+ A + NS +W ++L P++QE + EI + N +L K++MPYT A + E+QR H T ++ + Y IPK +LI+ NL+++H D W+ P+ P FL + G+ K ++ F+P+G+G R C+G ++ LF++ + ++ ++ ++ + ++ + + + PFNI R
Sbjct: 96 IDPSVIGPQVLLAHLARVYGSIFSFFIGHYLVVVLSDFHSVREALVQQ-AEVFSDRPRVPLISIVTKEKG-VVFAHYGPVWRQQRKFSHSTLRHFG-LGKLS-LEPKIIEEFKYVKAEMQKHGEDPFCPFSIISNAVSNIICSLCFGQRFDYTNSEFKKMLGFMSRGLEICLNSQVLLVNICPWLYYLP-----FGPFKELRQIEKDITSFLKKIIKDHQESLDRENPQ-DFIDMYLLHMEEE----RKNNSNSSFDEEYLFYIIGDLFIAGTDTTTNSLLWCLLYMSLNPDVQEKVHEEIERVIGANRAP-SLTDKAQMPYTEATIMEVQRLTVVVPLAIPHMTSENTVLQGYTIPKGTLILPNLWSVHRDPAIWEKPEDFYPNRFLDDQGQLIK-KETFIPFGIGKRVCMGEQLAKMELFLMFVSLMQSFAFALPEDSKKPLLTGRFGLTLAP-HPFNITISR 543
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Human
Match: CYP2R1 (cytochrome P450 family 2 subfamily R member 1 [Source:HGNC Symbol;Acc:HGNC:20580]) HSP 1 Score: 115.931 bits (289), Expect = 4.934e-27 Identity = 57/173 (32.95%), Postives = 93/173 (53.76%), Query Frame = 3
Query: 912 SLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHW--KSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKM 1424
S+ ++I A E N W +ALYPNIQ ++ EI+ N W K KMPYT AVL E+ R+ + FH+T +D + Y IPK + ++ NLY++H D+K W++P++ P+ FL G F +++ VP+ LG R C+G + +F+ ++ + +
Sbjct: 303 SVGELIIAGTETTTNVLRWAILFMALYPNIQGQVQKEIDLIMGPNGKP---SWDDKCKMPYTEAVLHEVLRFCNIVPLGIFHATSEDAVVRGYSIPKGTTVITNLYSVHFDEKYWRDPEVFHPERFLDSSG-YFAKKEALVPFSLGRRHCLGEHLARMEMFLFFTALLQRFHL 471
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Human
Match: CYP21A2 (cytochrome P450 family 21 subfamily A member 2 [Source:HGNC Symbol;Acc:HGNC:2600]) HSP 1 Score: 114.775 bits (286), Expect = 1.436e-26 Identity = 96/455 (21.10%), Postives = 195/455 (42.86%), Query Frame = 3
Query: 204 KYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKF-KETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFND-----VDYKKYQGAIEVIPQYAEECD--------FPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKK---GDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWK--SKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEPFNIQF 1511
K+GPI L+ VV +N T E V+K + +GR K++S N ++ DY LWK HK L A L + + M ++ +E E R + ++ + + ++I L F + D YK Q ++ ++ + FPN + +L++ + ++++++ +H++ G ++ D+ D++L V + ++ S +L + HV + +D++ E N+ W L +P IQ+ ++ E++ + +K +++P NA + E+ R H T +I Y+IP+ ++I+ NL H D+ W+ P P FL E G++ + + +G G R C+G + LFV++ ++ + ++ + + + I ++PF ++
Sbjct: 54 KFGPIYRLHLGLQDVVVLNSKRTIEEAMVKKWAD-FAGRPEPLTYKLVSKNYPDLSLGDYSLLWKA-----HKKLTRSALLLGIRDSMEPVVEQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLRFFPN---------PGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWR----DMMDYMLQGVAQPSMEEGSGQL----LEGHVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFL-EPGKNSRA----LAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRL 480
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Celegans
Match: cyp-33C9 (CYtochrome P450 family [Source:UniProtKB/TrEMBL;Acc:O16482]) HSP 1 Score: 103.219 bits (256), Expect = 3.807e-23 Identity = 105/445 (23.60%), Postives = 188/445 (42.25%), Query Frame = 3
Query: 180 KIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQR-NLHKGLRHCANLDRLNE-IMYKCFDKFKETVEYRRGSKNGGIEVD-NDLFWMA-DNVITMLLFNEIFNDVDYKKYQ-------------GAIEVIPQY-AEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIID 1460
K ++ KYG I T + P + ++ E E F++ + ++ E G+ + + GP W+ +R +H+ DR+ + IM + D F K G VD D+F A NVI +LF F++ +++ + + Q+ + P+ FK K QK F E +I+ H+K+ D+++ + +D+V ++E S + + + +D+ FA+ N+ W F Y + Q+ + E+++ + K +PYTNA + EIQR A+ H T D + Y IPK + +V + + +D+ + P I P+ FL +DG+ K+ + VP+ +G R C+G + LF+ I + Y++V N IID
Sbjct: 49 KAFARWTAKYGDIYTFWLGTRPYILVSSYEALKETFIKDGETYADKKPMAFQESFRGGSYGVVETN--GPFWREHRRFAIHQFRDFGLGKDRMEQRIMLEVEDIFNNC------DKTIGEGVDLTDIFDRAVGNVINQMLFGYRFDETRADEFRTIRAFFNFNSGEFASFSMRVQFFLPWMGYIMPGPTILDRFK---KYQKGFT---EFFGTQIENHKKEIDFELE--ENSDYVEAFLKEQRKREASGDFE-SFSTKQLSNMCLDLWFAALMTTSNTMTWCFAYTLNYLDAQQKLHEELDRVIGSER-HINTADKPNLPYTNAYINEIQRTANLVPLNLLHMTTRDTVLKGYNIPKGTGVVAQISTVMYDENVFPEPYIFKPERFLDDDGK-LKKVEQLVPFSVGKRQCLGEGLARMELFLFIANFFNRYRVVPDAN-GPPIID 473
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Celegans
Match: cyp-23A1 (CYtochrome P450 family [Source:UniProtKB/TrEMBL;Acc:Q27465]) HSP 1 Score: 99.3673 bits (246), Expect = 6.669e-22 Identity = 100/429 (23.31%), Postives = 187/429 (43.59%), Query Frame = 3
Query: 183 IVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKC-FDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFN-DVDYKKY-------QGAIEVIPQYAEECDFP--NDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHR---KKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLT---DLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNY 1418
+ Q + +YG I T++ P+V ++D+ T + F++ + S R ++ + N I+ D G W+ +R LR L E M +E G + + + N+I +LF FN D Y Q + P FP + P ++ +L + N+L+E L+ +I+ HR K + D+T ++ VE K K+ G + D +K L+D+ FA E + W F L+A +Q+N++ E++ + + ++++PY A + EIQR A+ + +DI I Y K LI+ + + +D + ++NP+ +P FL ED + K+ +F+P+ +G R C+G + + L+++ ++ N+
Sbjct: 81 LFQSWKQQYGGIFTVWIGPIPLVMVSDLPTIKKYFIQH-ADSFSNRWRNFVTDSIMEGSNGIVQID-GNKWREQRRFALHTLRDFGVGKPLMEQMITLEVTSLMNHMEKSCGLDGKELHLCPSIAVCVGNIINNMLFGLRFNQDNSYMHRLHQLLDDQSHTVMQPIMGAYIAFPVTSKIPIINGEWN---RLMGIKNELLEFLETQIEGHRMNWKDEMIEQEPEDLTYAYMIEVE------KRKRNGEDVGFFDDQQLKMLLLDLFFAGMETTVTTLKWAFLLMAKNQKVQKNVQAELDSI---GQPMIEIQHRTRLPYVQATINEIQRIANILPINLLRTVAEDIEIDGYNFKKGDLIIPQISILMNDPEIFENPEEFNPSRFLDEDN-NVKKIDEFLPFSIGRRQCLGESLARAELYLVFANLIQNF 494
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Celegans
Match: cyp-34A10 (CYtochrome P450 family [Source:UniProtKB/TrEMBL;Acc:O61204]) HSP 1 Score: 98.9821 bits (245), Expect = 9.150e-22 Identity = 105/425 (24.71%), Postives = 189/425 (44.47%), Query Frame = 3
Query: 207 YGPICTLYKDGTPVVFINDIETGYEVFVRK---LSKQVSGRSASY---GEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETV-EYRRGS-KNGGIEVDNDLFW--MADNVITMLLFNEIF--NDVDYKKYQGAIEVIPQYAEECD--FPN---DKPSKKSDFKSVEKLQKVFNQLIEVLQIRID---EHRKKGDYKI--ACFDITDFVLLAVE--EYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVV 1409
YG + T++ P V+I+D + +E +++ S + + + +Y G I++ N G W+ +R LR+ L R N + K +++ + +Y+R KNG IE+ F+ + ++I LL +E F D +++K + ++ + + D FP D P K K K+ + F+ + EV + + E K GD+ I D D +L ++ E P + L + D+ D+ A QE + W F L +PN+ + +R E+ K N V L+ ++ PY NAV E+QR AS F D I + ++ + +H D + +KNP P+ FL + K +P+G+G R+C G + + L++++ V+
Sbjct: 62 YGKVFTIWMGPLPSVYISDYDVAHESHIKRANIFSTRFAHGTTNYIREGRGIIAAN---------GDFWQDHRRFALTTLRNFG-LGR-NLMEEKIMEEYNYRISDYKRTHLKNGAIEIHAGTFFDLLVGSIINQLLISERFEQGDQEFEKIKTSLALSLENFGIIDIFFPTAILDSPLMKWRQK---KIFEPFDFIYEVSKRNLTKRMEAVKSGDHVIEDGGRDFVDAYILKIQKDENSGAPSTFNLETMIIDV------FDLWQAGQETTSTTLTWAFCCLLNHPNVVKKLRAELMKLTGGNR-HVGLNDRADTPYLNAVCNEVQRIASILNINLFRKIEQDTEIAGQPLASGCVVTTQMSMLHTDVEVYKNPTEFRPERFLENN----TLEKKLIPFGIGKRSCPGESLARAELYLILSNVI 461
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Celegans
Match: cyp-33C2 (CYtochrome P450 family [Source:UniProtKB/TrEMBL;Acc:O44706]) HSP 1 Score: 97.0561 bits (240), Expect = 3.573e-21 Identity = 107/477 (22.43%), Postives = 203/477 (42.56%), Query Frame = 3
Query: 111 LPPGPNRNSDEADF--IINEKLIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCA---NLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKS-----VEKLQKVF----------NQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFI 1481
PPGP + I N Y+ +++ KYG I T + TP + IN + E ++R ++ + + + G I++S+ G W+ +R +R NL + N I+ + D F ++ + GS+ EV + NV+ LLF F +YQ +I + P D K F + + KL F + +I+ HR++ D + TDFV ++E +S+ +++ + + +D+ FA N+ W + +P +Q+ + E++K + + +T K+ +PY NA + E QR + H+T D I ++IPK + +V + + ++++ + +P +P F+ E G+ KR ++ P+ +G R+C G + LF+ I ++ YK ++ + +D N I
Sbjct: 25 FPPGPCPLPVFGNLLSIANPPPGYEAF-ERWTKKYGDIYTFWIGNTPHIMINTWDKIKETYIRD-AETYTNKVRLPALDLFRGGEYGIIDSN-GATWREHRRFALSTMRDFGLGKNLMQEN-ILMEVQDVFAR-LDAKLGSETDIPEVFDH---AVANVVNQLLFGYRFVGPKESEYQELKRII-------EAPTDLFGKPHMFLAMNIPVIAKLMPEFMYEGPFKDFRDTAFAFFNKQIEAHRQEVDLNDLNSESTDFVETFLKEQKRR-ESEGDSETFSNVQLSNVCMDLWFAGLNTTTNTITWAISYILHHPEVQDKVHEEMDKVIGSDRL-ITTADKNDLPYFNAFINEAQRGINIVPLNLQHATTRDTVINGFKIPKGTGVVAQISTVMNNEEVFPDPHTFNPDRFIDEHGK-LKRVEELAPFSVGKRSCPGEGLARMELFLFIANFLNRYK-IYPSKEGPPSMDKSNGALI 482
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Celegans
Match: cyp-31A3 (CYtochrome P450 family [Source:UniProtKB/TrEMBL;Acc:Q9N574]) HSP 1 Score: 96.2857 bits (238), Expect = 6.605e-21 Identity = 70/277 (25.27%), Postives = 129/277 (46.57%), Query Frame = 3
Query: 702 DFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKI-ACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQR-YASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDD---KNAVFIRDVEPFNIQFIR 1517
D ++ EK ++ + + + + E ++ DYK+ D +L V+ +D + V+ + +F + +W LL +P +Q ++ E+++ D+ +VT+ S+M Y L E R + S T S DD IG IPK +LNLY +H D QWK+PD+ DP FL E+ + ++ F+P+ G R C+G F+ V++ ++ N+ + K+ E++ + K + +R V P +++ R
Sbjct: 232 DGRTHEKCLRILHDFTKKVIVERKEALQENDYKMEGRLAFLDLLLEMVKSGQMD-----------ETDVQAEVDTFMFEGHDTTSTGLMWAIHLLGNHPEVQRKVQAELDEVMGDDE-DVTIEHLSRMKYLECALKEALRLFPSVPIITRELS--DDQVIGGVNIPKGVTFLLNLYLVHRDPSQWKDPDVFDPDRFLPENSIA-RKSFAFIPFSAGSRNCIGQRFALMEEKVIMAHLLRNFNV-----KAVELMHEVRPKMEIIVRPVTPIHMKLTR 488
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Fly
Match: Cyp305a1 (gene:FBgn0036910 transcript:FBtr0074925) HSP 1 Score: 96.6709 bits (239), Expect = 6.117e-21 Identity = 47/204 (23.04%), Postives = 104/204 (50.98%), Query Frame = 3
Query: 912 SLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHW--KSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEPFNIQFIR 1517
+++D A + N+ +LA+ P++QE + +++ + + H + Y +A + E+QR+ T + +G Y+IPK++ I+++L ++H DK+ WK+P P+ F+ G+ FK + F+P+G+G R C+G+ + +F ++ +V ++ +V +S ++ +P+ +QF++
Sbjct: 302 TILDFFIAGSQTTSNTINLALMVLAMRPDVQEKLFSQVTASVAAASTDAFPHLSRREAFDYMDAFIMEVQRFFHITPITGPRRALWATKLGGYDIPKNATILISLRSVHLDKEHWKDPLEFRPERFIDSAGKCFK-DEYFMPFGMGRRRCLGDALARACIFSFLVRIVQHFSVVLPAGESPSMVLLPGITL--TPKPYKVQFVK 502
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Fly
Match: Cyp304a1 (gene:FBgn0038095 transcript:FBtr0082691) HSP 1 Score: 95.5153 bits (236), Expect = 1.294e-20 Identity = 50/176 (28.41%), Postives = 87/176 (49.43%), Query Frame = 3
Query: 912 SLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKN 1439
L+D F + +G + L LYP + ++ EI++ + L + +P+T A + E R + H ++D + Y IPKD+++V +LYA H D + W +P+ P+ FL DG+ + +P+G G R C G TF+ N+LF++ + ++ V G N
Sbjct: 308 GLVDFSFPAFTAIGVQLSLLVQYLMLYPAVLRRVQNEIDEVVGCGRLP-NLEDRKNLPFTEATIREGLRIETLVPSDVPHKALEDTELLGYRIPKDTIVVPSLYAFHSDARIWSDPEQFRPERFLDADGKLCLKLDVSLPFGAGKRLCAGETFARNMLFLVTATMCQHFDFVLGPN 482
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Fly
Match: Cyp18a1 (gene:FBgn0010383 transcript:FBtr0074610) HSP 1 Score: 90.5077 bits (223), Expect = 7.089e-19 Identity = 91/445 (20.45%), Postives = 188/445 (42.25%), Query Frame = 3
Query: 108 KLPPGPNRNSDEADFIINEKLIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLN---EIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFN--DVDYKKYQ----------GAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKL-GIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVL 1394
KLPPGP + + + ++ +YG + + V ++D + E F R+ + +GR + + L+G I+NS G LWK +R LH LR N ++ + + E + + S +++ + NVI L+ + F+ D ++++ G I + FP+ +K ++ ++Q+ + +I D+H++ D D+ DF L +E+ + +L K + + +ID+ A E + + +W+ + P ++ E+++ + + T+ +P T + + E R +S HS D+ + Y IP S ++ + ++H D W+ P+ P F+ +G+ ++ + F+P+G+G R C+G+ + LF+
Sbjct: 52 KLPPGPWGLPVIGYLLFMGSEKHTRFME-LAKQYGSLFSTRLGSQLTVVMSDYKMIRECFRRE---EFTGRPDTPFMQTLNGY--GIINST-GKLWKDQRRFLHDKLRQFGMTYMGNGKQQMQKRIMTEVHEFIGHLHASDGQPVDMSPVISVAVSNVICSLMMSTRFSIDDPKFRRFNFLIEEGMRLFGEIHTVDYIPTMQCFPSISTAKNKIAQNRAEMQRFYQDVI-------DDHKRSFDPN-NIRDLVDFYLCEIEKAKAEGTDAELFDGKNHEEQLVQVIIDLFSAGMETIKTTLLWINVFMLRNPKEMRRVQDELDQVVGRHRLP-TIEDLQYLPITESTILESMRRSSIVPLATTHSPTRDVELNGYTIPAGSHVIPLINSVHMDPNLWEKPEEFRPSRFIDTEGK-VRKPEYFIPFGVGRRMCLGDVLARMELFLF 479
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Fly
Match: Cyp18a1 (gene:FBgn0010383 transcript:FBtr0074609) HSP 1 Score: 90.5077 bits (223), Expect = 7.089e-19 Identity = 91/445 (20.45%), Postives = 188/445 (42.25%), Query Frame = 3
Query: 108 KLPPGPNRNSDEADFIINEKLIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLN---EIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFN--DVDYKKYQ----------GAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKL-GIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVL 1394
KLPPGP + + + ++ +YG + + V ++D + E F R+ + +GR + + L+G I+NS G LWK +R LH LR N ++ + + E + + S +++ + NVI L+ + F+ D ++++ G I + FP+ +K ++ ++Q+ + +I D+H++ D D+ DF L +E+ + +L K + + +ID+ A E + + +W+ + P ++ E+++ + + T+ +P T + + E R +S HS D+ + Y IP S ++ + ++H D W+ P+ P F+ +G+ ++ + F+P+G+G R C+G+ + LF+
Sbjct: 52 KLPPGPWGLPVIGYLLFMGSEKHTRFME-LAKQYGSLFSTRLGSQLTVVMSDYKMIRECFRRE---EFTGRPDTPFMQTLNGY--GIINST-GKLWKDQRRFLHDKLRQFGMTYMGNGKQQMQKRIMTEVHEFIGHLHASDGQPVDMSPVISVAVSNVICSLMMSTRFSIDDPKFRRFNFLIEEGMRLFGEIHTVDYIPTMQCFPSISTAKNKIAQNRAEMQRFYQDVI-------DDHKRSFDPN-NIRDLVDFYLCEIEKAKAEGTDAELFDGKNHEEQLVQVIIDLFSAGMETIKTTLLWINVFMLRNPKEMRRVQDELDQVVGRHRLP-TIEDLQYLPITESTILESMRRSSIVPLATTHSPTRDVELNGYTIPAGSHVIPLINSVHMDPNLWEKPEEFRPSRFIDTEGK-VRKPEYFIPFGVGRRMCLGDVLARMELFLF 479
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Fly
Match: Cyp303a1 (gene:FBgn0001992 transcript:FBtr0343812) HSP 1 Score: 80.8777 bits (198), Expect = 8.077e-16 Identity = 53/199 (26.63%), Postives = 89/199 (44.72%), Query Frame = 3
Query: 918 IDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEPFNIQFI 1514
+D+ A E S + F L L P IQE EI + + ++K+PY A+ E R TF H + D + YEIPKD++++ + + + +P+ +P +L DG K + F P+G G C+G+ LF+ V+ N+KMV + E + + A V+P++I +
Sbjct: 305 LDMFLAGSETTNKSLGFCFMHLVLQPEIQERAFQEIKEVVGLERIPEWSRDRTKLPYCEAITLEAVRMFMLHTFGIPHRAVCDTRLSGYEIPKDTMVIACFRGMLINPVDFPDPESFNPDRYLF-DGH-LKLPEAFNPFGFGRHRCMGDLLGRQNLFMFTTTVLQNFKMVAIPGQVPEEVPLEGAT--AAVKPYDIMLV 499
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Zebrafish
Match: cyp2x8 (cytochrome P450, family 2, subfamily X, polypeptide 8 [Source:ZFIN;Acc:ZDB-GENE-040704-28]) HSP 1 Score: 145.976 bits (367), Expect = 1.252e-37 Identity = 127/457 (27.79%), Postives = 220/457 (48.14%), Query Frame = 3
Query: 111 LPPGPNRNSDEADFIINEKLIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFW--MADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKL--QKVFNQLIEVLQIR----IDEH---RKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQ 1448
PPGP+ + + K +++ KYG I +LY P VF+N E V K ++ SGR + L+ + +L +DYGPLWK +R LR+ + E + + V Y KN G VD + + +A NVI+++LF F D + + Q I++I + ++ + P + F + L +K F+ ++VL+ IDEH R G + DF+ ++E LD + ++ D + + ++D+ FA + N+ + F L +P +Q + EI+ + + +V+ + MPYT AV+ E+QR A+ + FH T D + Y IPK +LI+ NL + ++ QWK P +P NFL+E G+ F++ + F+P+ G R C+G + LF++ + ++ YK VW ++ +
Sbjct: 27 FPPGPSPLPIFGNLLHFNLANPLKEFERFAEKYGNIFSLYTGSRPAVFLNSFAVIKEALVTK-AQDFSGRPQDFMISHLTECKGVVL-ADYGPLWKDHRRFALMTLRNFGLGKQSME--ERILGEISHVVGYL--DKNIGKTVDPQVMFHNVASNVISLVLFGRRF-DYNSETLQCYIQLITEISKILNGPWNMIYDTLPFLRILPLPFKKGFDH-VKVLKGMNLKLIDEHKSTRVPGKPR-------DFIDCYLDE--LDKRKNEVSTFSEDQLLMY-ILDLYFAGTDTTSNTLLTAFLYLMNHPEVQVKCQQEIDDVLEGKD-QVSYEDRDNMPYTLAVIHEVQRVANTVPLSVFHCTTRDTELMGYSIPKGTLIIPNLTIVLKEEGQWKFPHEFNPANFLNEQGQ-FEKPEAFIPFSTGPRVCLGEGLARMELFLVFVTLLRRYKFVWPRDAGK 463
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Zebrafish
Match: cyp2x8 (cytochrome P450, family 2, subfamily X, polypeptide 8 [Source:ZFIN;Acc:ZDB-GENE-040704-28]) HSP 1 Score: 145.591 bits (366), Expect = 1.872e-37 Identity = 126/457 (27.57%), Postives = 220/457 (48.14%), Query Frame = 3
Query: 111 LPPGPNRNSDEADFIINEKLIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFW--MADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKL--QKVFNQLIEVLQIR----IDEH---RKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQ 1448
PPGP+ + + K +++ KYG I +LY P VF+N E V K ++ SGR + L+ + +L +DYGPLWK +R LR+ + E + + V Y KN G VD + + +A NVI+++LF F D + + Q I++I + ++ + P + F + L +K F+ ++VL+ IDEH R G + DF+ ++E LD + ++ ++ + ++D+ FA + N+ + F L +P +Q + EI+ + + +V+ + MPYT AV+ E+QR A+ + FH T D + Y IPK +LI+ NL + ++ QWK P +P NFL+E G+ F++ + F+P+ G R C+G + LF++ + ++ YK VW ++ +
Sbjct: 27 FPPGPSPLPIFGNLLHFNLANPLKDFERFAEKYGNIFSLYTGSRPAVFLNSFAVIKEALVNK-AQDFSGRPQDFMISHLTECKGVVL-ADYGPLWKDHRRFALMTLRNFGLGKQSME--ERILGEISHVVCYL--DKNIGKTVDPQVMFHNVASNVISLVLFGRRF-DYNSETLQCYIQLITEISKILNGPWNMIYDTLPFLRILPLPFKKGFDH-VKVLKGMNLKLIDEHKSTRVPGKPR-------DFIDCYLDE--LDKRKNEVS-TFSEDQLLMYILDLYFAGTDTTSNTLLTAFLYLMNHPEVQVKCQQEIDDVLEGKD-QVSYEDRDNMPYTLAVIHEVQRVANTVPLSVFHCTTRDTELMGYSIPKGTLIIPNLTIVLKEEGQWKFPHEFNPANFLNEQGQ-FEKPEAFIPFSTGPRVCLGEGLARMELFLVFVTLLRRYKFVWPRDAGK 463
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Zebrafish
Match: cyp2x7 (cytochrome P450, family 2, subfamily X, polypeptide 7 [Source:ZFIN;Acc:ZDB-GENE-110114-2]) HSP 1 Score: 142.51 bits (358), Expect = 2.361e-36 Identity = 126/454 (27.75%), Postives = 218/454 (48.02%), Query Frame = 3
Query: 189 QKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFW--MADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECDFPNDK-------------PSKKSDFKSVEKLQKVFNQLIEVLQIRIDEH---RKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEP 1496
++ KYG I +LY P VF+N+ E E V K ++ SGR L+GN+ +L +DYGPLWK +R LR+ + E + + V++ KN G VD + + +A NVI ++LF F D + + +G I+ I + + P + P KK+ F +V+ ++ + +LI DEH R G + DF+ ++E LD K K ++ + +++D+ FA + N+ + F L +P++Q + EI+ + + +VT + MPYT AV+ E+QR A+ + H T D + Y IPK ++I+ NL + ++ QWK P +P NFL+E G+ F++ + F+P+ G R C+G + LF++ + ++ ++ VW ++ + D VF + P
Sbjct: 53 ERLAEKYGNIFSLYTGSKPAVFLNNFEVIKEALVTK-AQDFSGRPQDLMISHLTGNKGVVL-ADYGPLWKDHRRFALMTLRNFGLGKQSME--ERILGEISHIVDFL--DKNTGKTVDPQIMFHNIASNVINLVLFGCRF-DYNNEFLRGYIQRIAENLRILNGPWNMIYDTFPLLRILPLPFKKA-FDNVKIIKSMNRKLI-------DEHKSTRVPGQPR-------DFIDCYLDE--LD-KVKNCVSTFSEDQLIMNIMDMSFAGTDTTSNTLLAAFLYLMNHPDVQVKCQQEIDDVLEGKD-QVTYEDRHNMPYTLAVIHEVQRVANIVPLSVLHCTTRDTELMGYSIPKGTVIIPNLTVVLKEEGQWKFPHEFNPANFLNEQGQ-FEKPEAFIPFSTGPRVCLGEGLARMELFLIFVTLLRRFQFVWPEDAGKP---DYTPVFGLTMTP 476
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Zebrafish
Match: cyp2x6 (cytochrome P450, family 2, subfamily X, polypeptide 6 [Source:ZFIN;Acc:ZDB-GENE-050320-144]) HSP 1 Score: 141.739 bits (356), Expect = 3.303e-36 Identity = 120/443 (27.09%), Postives = 217/443 (48.98%), Query Frame = 3
Query: 189 QKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFW--MADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKL--QKVFN--QLIEVLQIR-IDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEP 1496
+K+ KYG I +LY P V +N E V K ++ SGR + + N+ +L +DYGP+ K +R +R+ + E + + V+Y KN G VD + + +A NVI++LLF F D + + Q I++ + ++ + P + + L +K F+ ++I+ + ++ IDEH+ DF+ ++E LD K K ++ + S++D+ FA + + N+ + F L +P +Q + EI+ + + +VT + MPYT AV+ E+QR A+ + FH T D + Y IPK ++I+ NL ++ ++ QWK P +P NFL+E G+ F++ + F+P+ GLR C+G + LF++ + ++ ++ VW ++ + D VF + P
Sbjct: 53 EKFAEKYGEIFSLYTGSRPAVILNSFAVIKEALVTK-AQDFSGRPQDFMISHATENKGIVL-ADYGPVLKDHRRFALMTMRNFGLSKQSME--ERILGEISHVVDYL--DKNAGKRVDPHIMFHNVASNVISLLLFGCRF-DYNSEFLQCYIQLSNEISKIINGPWNMIYDTFPLLRILPLPFKKAFDHVKVIKSMNLKLIDEHKSTRP--------RDFIDCYLDE--LD-KGKNCVSTFSEDKLLMSIMDLHFAGTDTISNTLLTAFLYLMNHPEVQVKCQQEIDDVLEGKD-QVTYEDRHNMPYTLAVIHEVQRVANTVPLSVFHCTTRDTELMGYSIPKGTIIIPNLTSVLKEEGQWKFPHEFNPANFLNEQGQ-FEKPEAFIPFSTGLRMCLGEGLARMELFLIFVTLLRRFQFVWPEDAGKP---DYTPVFGLTLTP 472
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Zebrafish
Match: cyp2x7 (cytochrome P450, family 2, subfamily X, polypeptide 7 [Source:ZFIN;Acc:ZDB-GENE-110114-2]) HSP 1 Score: 141.739 bits (356), Expect = 3.663e-36 Identity = 126/454 (27.75%), Postives = 217/454 (47.80%), Query Frame = 3
Query: 189 QKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFW--MADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECDFPNDK-------------PSKKSDFKSVEKLQKVFNQLIEVLQIRIDEH---RKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEP 1496
++ KYG I +LY P VF+N+ E E V K ++ SGR L+GN+ +L +DYGPLWK +R LR+ + E + + V++ KN G VD + + +A NVI ++LF F D + + +G I+ I + + P + P KK+ F +V+ ++ + +LI DEH R G + DF+ ++E LD K K ++ + +++D+ FA + N+ + F L +P +Q + EI+ + + +VT + MPYT AV+ E+QR A+ + H T D + Y IPK ++I+ NL + ++ QWK P +P NFL+E G+ F++ + F+P+ G R C+G + LF++ + ++ ++ VW ++ + D VF + P
Sbjct: 53 ERLAEKYGNIFSLYTGSKPAVFLNNFEVIKEALVTK-AQDFSGRPQDLMISHLTGNKGVVL-ADYGPLWKDHRRFALMTLRNFGLGKQSME--ERILGEISHIVDFL--DKNTGKTVDPQIMFHNIASNVINLVLFGCRF-DYNNEFLRGYIQRIAENLRILNGPWNMIYDTFPLLRILPLPFKKA-FDNVKIIKSMNRKLI-------DEHKSTRVPGQPR-------DFIDCYLDE--LD-KVKNCVSTFSEDQLIMNIMDMSFAGTDTTSNTLLAAFLYLMNHPEVQVKCQQEIDDVLEGKD-QVTYEDRHNMPYTLAVIHEVQRVANIVPLSVLHCTTRDTELMGYSIPKGTVIIPNLTVVLKEEGQWKFPHEFNPANFLNEQGQ-FEKPEAFIPFSTGPRVCLGEGLARMELFLIFVTLLRRFQFVWPEDAGKP---DYTPVFGLTMTP 476
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Xenopus
Match: ENSXETT00000043988.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:4:50332813:50396253:-1 gene:ENSXETG00000002382.1 transcript:ENSXETT00000043988.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 127.487 bits (319), Expect = 4.533e-31 Identity = 106/457 (23.19%), Postives = 212/457 (46.39%), Query Frame = 3
Query: 111 LPPGPNRNSDEADFIINEKLIYKKIVQKY--IPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWK-----TLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFN---DVDYKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKL----QKVFNQL---IEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVW 1430
LPP P R++ +D+++ + ++ Y + YGP+ ++Y TP V + +++ EV V K +GR + ++S ++ ++ + YG WK TL GL + DR+ E FK V + D ++ +N ++ ++ + +F D + +Q + ++ + + P+ + F ++ L +K+F + L ++EH++ + + D++ +EE L G+ + ++ + D+ A E +S W + +P IQE EI++ + N + + +MPYT AVL E+QR+AS HS + D+++ + IPK ++I+ +L ++H+DK WK P +P+NFL+E+G K + F+P+ G R C+G + +F+ ++ +++ W
Sbjct: 4 LPPSPGRSA--SDWLVRMFSLEQETGSCYTHVHLYGPVYSIYLGFTPAVVVRGLKSIKEVLVNK-GTDFAGRPQNRITDVISKSKGLVV-APYGQAWKEHRRFTLSTLRSFGLGKKSMEDRIQEENMHLIQAFKNNVLF-------------DPHFVLENAVSNIICSIVFGRRYDYNDTSFQNILNLVHENMKLSS-PSTSCLLYNAFGFIQFLPLPHKKIFQNVDVVFAFLGKVVEEHKET----LLPGEPRDYIDCYLEE--LKKADATRGMTFDEENLFSCVADLFVAGTETTTSSLEWCLLYMLKFPEIQEKCHEEIDRVFGNKNC-LEYEDRERMPYTQAVLQEVQRHASVVPLGVSHSPIRDVHLNGFFIPKGTVIITDLSSVHYDKTHWKYPHEFNPENFLNENGELIK-TEAFLPFSAGPRVCLGENLARMEIFLFFTAMLQHFEFHW 434
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Xenopus
Match: foxh1.2 (forkhead box H1, gene 2 [Source:Xenbase;Acc:XB-GENE-478064]) HSP 1 Score: 122.865 bits (307), Expect = 2.176e-29 Identity = 118/469 (25.16%), Postives = 205/469 (43.71%), Query Frame = 3
Query: 108 KLPPGPNRNSDEADFI-INEKLIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQR-------NLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFW--MADNVITMLLFNEIFN--DVDYKKYQGAIE---------------VIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSL---IDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKM 1424
KLPPGP +F+ + + ++K +++ + KYGP+ T++ PVV + + E + + EKI G + ++ G WK L+R N G R +R+ E + ++FK T G VD ++ NVI ++F + F D ++ + G + + P+ + P++ FKSV LQ+ Q I + Q +D + D D L+ +++ P+ T+ H++ +L D+ A E + + + +L +P+I+E I+ EI+ N + +S+MPYT+AV+ EIQR+ H DI Y IPK + + L ++ HD KQ+K PDI +P +F+ E+G+ F + F+P+ G R CVG + LF+ I ++ NY +
Sbjct: 33 KLPPGPTPLPFLGNFLQLRGEEVFKSLLE-FGKKYGPVYTIHLGMEPVVVLCSFDIVKEALNDNGDEFGARGHMPLLEKISHGGHGVVASN--GERWKQLRRFSLMTLRNFGMGKRSIE--ERIQEEAHFLTNEFKYT---------KGQPVDPTFYFSKAVSNVICSVVFGDRFEYEDTEFLRLLGLLNQVFRGFSSVWGQLYNIFPKVMGKLPGPHNM-----IFKSVNSLQEFIMQRINMHQETLDPSSPR--------DFIDCFLIKMQQEKDVPQ--------TEFHMQGALNTTFDMFGAGTETVSTTLRYGLLILLKHPDIEERIQKEIDSVIGRNRAPC-IEDRSRMPYTDAVIHEIQRFVDIIPMGIPHKVTRDIQFQGYFIPKGTTVYPMLSSVLHDPKQFKYPDIFNPGHFIDENGK-FCKNDGFMPFSSGKRICVGEGLARMELFLFITTILQNYTL 464
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Xenopus
Match: cyp2u1 (cytochrome P450 family 2 subfamily U member 1 [Source:NCBI gene;Acc:100127656]) HSP 1 Score: 122.479 bits (306), Expect = 4.066e-29 Identity = 117/485 (24.12%), Postives = 223/485 (45.98%), Query Frame = 3
Query: 108 KLPPGPNRNSDEADFIINEKLIYKKIVQKYIPK-YGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGG---IEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAE-----------ECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEPFNIQFIR 1517
++P G R +Y IV + K YG I LY +V +ND + + V S+ S R + I++ R I+ + YGP+W+ +R H LR+ L +L+ + K ++FK N G E+ N+ NVI + F + FN D K+++ + ++ + E C + P F ++L+++ + L+ I EH+ D D D LL ++E + K + I T+ ++ + + D+ A + N+ +W + LYP++QE ++ EI+ + +L KS+MP+T A + E+QR + H + Y IPK S+++ NL+++H D K W+ P+ P+ FL E+G+ K ++ F+P+G+G R C+G ++ LF++ + ++ ++ + + ++ + + + PF+I+ +
Sbjct: 100 RVPAGATRRG---------AFLYPHIVLTEMAKMYGKIYGLYIGTRLMVILNDFNSVKDALVSH-SEVFSDRPSVSLVTIIT-KRKGIVFAPYGPIWRQQRRFSHSTLRYFG-LGKLS-LEPKIIEEFKYVKAEMLKFGNKGFSPFEIINN---AVSNVICSISFGKRFNYED-KEFKTMLSLMSRGLEISVNSEAVLICLCSWLYYLP-----FGPFKELRQIVIDITAFLKRIIAEHQVTLD-PANPRDFIDMYLLHIKE---EQKGQAESIFNTE-YLFYIIGDLFIAGTDTTTNTLLWSLLYMCLYPDVQEKVQAEIDTVIGRDR-PPSLTDKSQMPFTEATIMEVQRMTVVVPLSVPHMASESSVFHGYTIPKGSVVMANLWSVHRDPKVWEKPNDFMPKRFLDENGQILK-KEAFIPFGIGRRVCMGEQLAKMELFLMFVNLLQSFSFSLADDTFKPSLEGRFGLTLAPY-PFDIKITK 554
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Xenopus
Match: cyp17a1 (cytochrome P450 family 17 subfamily A member 1 [Source:NCBI gene;Acc:100145650]) HSP 1 Score: 121.709 bits (304), Expect = 5.304e-29 Identity = 100/437 (22.88%), Postives = 200/437 (45.77%), Query Frame = 3
Query: 204 KYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCA-NLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVD------YKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKK--GDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRS-FKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIR 1484
KYG + + +V +N E EV ++K K GR + +L+ N +I +DY P WK ++ +H L + +I+ + +T+ +GS +++ +L NV+ L FN + D +G ++ + + + FP + D + +++ + ++L LQ ++ EH++ G+ D L++E + + S+++G LTD H+ ++ D+ A E W L YP +Q I+ E++ + L + +PY +A + E+ R H + + +IG Y IP+D+ +V+NL+++HHD +W+NP+ P FL E+G + + ++P+G G+R C+G ++ +F+ + ++ + + S +D K V ++
Sbjct: 39 KYGSLYSFKMGSHYIVIVNHHEHAKEVLLKK-GKTFGGRPRAVTTDLLTRNAKDIAFADYSPTWKFHRKLVHAALSMFGEGTVAIEKIISREAASLCQTLITFQGSP---LDMAPELTRAVTNVVCALCFNARYKRCDPEFEEMLAYSKGIVDTVAKDSLVDIFPWLQIFPNKDLEILKRSVAIRDKL---LQKKLKEHKEAFCGEEVNDLLDALLKAKLSMENNNSN-ISQEVG--LTDDHLLMTVGDIFGAGVETTTTVLKWTVAYLLHYPKVQAKIQEELD-VKVGFGRHPVLSDRRILPYLDATISEVLRIRPVAPLLIPHVALHESSIGEYTIPQDARVVINLWSLHHDPNEWENPEEFIPDRFLDENGNHLYTPSQSYLPFGAGIRVCLGEALAKMEIFLFLSWILQRFTLEVPAGDSLPDLDGKFGVVLQ 464
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Xenopus
Match: cyp17a1 (cytochrome P450 family 17 subfamily A member 1 [Source:NCBI gene;Acc:100145650]) HSP 1 Score: 121.324 bits (303), Expect = 7.128e-29 Identity = 100/437 (22.88%), Postives = 200/437 (45.77%), Query Frame = 3
Query: 204 KYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCA-NLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVD------YKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKK--GDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRS-FKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIR 1484
KYG + + +V +N E EV ++K K GR + +L+ N +I +DY P WK ++ +H L + +I+ + +T+ +GS +++ +L NV+ L FN + D +G ++ + + + FP + D + +++ + ++L LQ ++ EH++ G+ D L++E + + S+++G LTD H+ ++ D+ A E W L YP +Q I+ E++ + L + +PY +A + E+ R H + + +IG Y IP+D+ +V+NL+++HHD +W+NP+ P FL E+G + + ++P+G G+R C+G ++ +F+ + ++ + + S +D K V ++
Sbjct: 65 KYGSLYSFKMGSHYIVIVNHHEHAKEVLLKK-GKTFGGRPRAVTTDLLTRNAKDIAFADYSPTWKFHRKLVHAALSMFGEGTVAIEKIISREAASLCQTLITFQGSP---LDMAPELTRAVTNVVCALCFNARYKRCDPEFEEMLAYSKGIVDTVAKDSLVDIFPWLQIFPNKDLEILKRSVAIRDKL---LQKKLKEHKEAFCGEEVNDLLDALLKAKLSMENNNSN-ISQEVG--LTDDHLLMTVGDIFGAGVETTTTVLKWTVAYLLHYPKVQAKIQEELD-VKVGFGRHPVLSDRRILPYLDATISEVLRIRPVAPLLIPHVALHESSIGEYTIPQDARVVINLWSLHHDPNEWENPEEFIPDRFLDENGNHLYTPSQSYLPFGAGIRVCLGEALAKMEIFLFLSWILQRFTLEVPAGDSLPDLDGKFGVVLQ 490
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Mouse
Match: Cyp2j6 (cytochrome P450, family 2, subfamily j, polypeptide 6 [Source:MGI Symbol;Acc:MGI:1270148]) HSP 1 Score: 123.25 bits (308), Expect = 1.069e-29 Identity = 109/426 (25.59%), Postives = 195/426 (45.77%), Query Frame = 3
Query: 186 VQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCA-NLDRLNEIMYKCFDKFKETVEYRRGSK-NGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQ--YAEEC---DFPNDKP--------SKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNY 1418
+Q + KYG I +L V I + E ++ + + R S ++ +S N+N ++ S G +WK +R LR+ L E M + E + G N ++N + N+I + F E F+ D ++Q + ++ + Y E N P S + F++ EKL K+F + ID+HRK + + DF+ ++E P+ + ++ S +D+ FA E + W +ALYP +QE ++ EI++ L + MPYTNAV+ E+QR + D N+ + +PK ++++ NL A+H D K+W PD+ +P++FL E+G+ FK+R+ F+P+ +G R C+G + + LF+ ++ +
Sbjct: 68 IQPLVKKYGNIFSLNLGDITSVVITGLPLIKEALT-QMEQNIMNRPLSVMQERIS-NKNGLIFSS-GQIWKEQRRFALMTLRNFGLGKKSLEERMQEEASHLVEAIREEEGKPFNPHFSINNAV----SNIICSVTFGERFDYHD-SRFQEMLRLLDEVMYLETTMISQLYNIFPWIMKYIPGSHQKVFRNWEKL-KLF------VSCMIDDHRKDWNPD----EPRDFIDAFLKEMTKYPEKT---TSFNEENLICSTLDLFFAGTETTSTTLRWALLYMALYPEVQEKVQAEIDRVIGQKRA-ARLADRESMPYTNAVIHEVQRMGNIIPLNVPREVAMDTNLNGFHLPKGTMVLTNLTALHRDPKEWATPDVFNPEHFL-ENGQ-FKKRESFLPFSMGKRACLGEQLARSELFIFFTSLMQKF 468
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Mouse
Match: Cyp2r1 (cytochrome P450, family 2, subfamily r, polypeptide 1 [Source:MGI Symbol;Acc:MGI:2449771]) HSP 1 Score: 117.472 bits (293), Expect = 4.654e-29 Identity = 57/171 (33.33%), Postives = 94/171 (54.97%), Query Frame = 3
Query: 912 SLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKM 1424
S+ ++I A E N W +ALYPNIQ + EI+ N + +K KMPYT AVL E+ R+ + FH+T +D + Y IPK + ++ NLY++H D+K WK+PD+ P+ FL +G F +++ +P+ LG R C+G + +F+ ++ + +
Sbjct: 94 SVGELIIAGTETTTNVLRWAILFMALYPNIQGQVHKEIDLIVGHNR-RPSWEYKCKMPYTEAVLHEVLRFCNIVPLGIFHATSEDAVVRGYSIPKGTTVITNLYSVHFDEKYWKDPDMFYPERFLDSNG-YFTKKEALIPFSLGRRHCLGEQLARMEMFLFFTSLLQQFHL 262
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Mouse
Match: Cyp2j9 (cytochrome P450, family 2, subfamily j, polypeptide 9 [Source:MGI Symbol;Acc:MGI:1921769]) HSP 1 Score: 119.783 bits (299), Expect = 2.051e-28 Identity = 115/462 (24.89%), Postives = 201/462 (43.51%), Query Frame = 3
Query: 87 NRTISSYKLPPGPNRNS-DEADFIINEKLIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGS--KNGGIEVDN--DLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQY-----AEECDFPNDKP--------SKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNY 1418
NR +Y PPGP R F ++ K + + Q+++ KYG + +L P V I + E+F ++ R + K L I +SD WK +R LR+ R E ++ +E Y + GG D ++ N+I + F E F+ D ++Q + ++ + + C N P S ++ F++ KLQ + ++ + HR+ D D D L + +Y K + ++ S +D+ FA E + W +ALYP +QE ++ EI++ + L + MPYTNAV+ E+QR + F D + + +PK +++ NL A+H D +W PD +P++FL E+G+ FK+R+ F+P+ +G R C+G + + LF+ I + +
Sbjct: 37 NRRPKNY--PPGPMRLPFVGCLFHLDPKQPHLSL-QQFVKKYGNVLSLDFANIPSVVITGMPLIKEIFT-QMEHNFMNRPVTLLRKHLFNKNGLIFSSDQT--WKEQRRFALMTLRNFGLGKRSLE------ERIQEEAHYLVEAIRDEGGQPFDPHFNISNAVSNIICSITFGERFDYHD-SQFQEMLRLLDEAMCLESSMMCQLYNIFPRILQYLPGSHQTLFRNWRKLQLFVSDIV-------NNHRRDWDPD-EPRDFIDAFLTEMTKY-----PDKTTTSFNEENLICSTLDLFFAGTETTSTTLRWALLCMALYPEVQEKMQAEIDRVIGQAR-QPNLADRDSMPYTNAVIHEVQRMGNIIPFNVPREVAVDTQLAGFNLPKGIMVLSNLTALHRDPSEWTTPDTFNPEHFL-ENGQ-FKKRESFLPFSMGKRACLGEQLARSELFIFITTLFQKF 469
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Mouse
Match: Cyp2r1 (cytochrome P450, family 2, subfamily r, polypeptide 1 [Source:MGI Symbol;Acc:MGI:2449771]) HSP 1 Score: 118.242 bits (295), Expect = 7.128e-28 Identity = 57/171 (33.33%), Postives = 94/171 (54.97%), Query Frame = 3
Query: 912 SLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKM 1424
S+ ++I A E N W +ALYPNIQ + EI+ N + +K KMPYT AVL E+ R+ + FH+T +D + Y IPK + ++ NLY++H D+K WK+PD+ P+ FL +G F +++ +P+ LG R C+G + +F+ ++ + +
Sbjct: 303 SVGELIIAGTETTTNVLRWAILFMALYPNIQGQVHKEIDLIVGHNR-RPSWEYKCKMPYTEAVLHEVLRFCNIVPLGIFHATSEDAVVRGYSIPKGTTVITNLYSVHFDEKYWKDPDMFYPERFLDSNG-YFTKKEALIPFSLGRRHCLGEQLARMEMFLFFTSLLQQFHL 471
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Mouse
Match: Cyp2c29 (cytochrome P450, family 2, subfamily c, polypeptide 29 [Source:MGI Symbol;Acc:MGI:103238]) HSP 1 Score: 117.857 bits (294), Expect = 7.938e-28 Identity = 118/453 (26.05%), Postives = 213/453 (47.02%), Query Frame = 3
Query: 108 KLPPGPNRNSDEADFIINEKLIYKKIVQKYI---PKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGR-SASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECDFP-----NDKPSKKSDF--KSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKH---SLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKM 1424
KLPPGP +F+ ++ K I Q + YGP+ TLY P V ++ E E + + ++ +GR S EKI+ G N G WK ++R LR+ R I + ++ + VE R +K + L NVI ++F F+ D K++ ++ I + + P N PS D+ S K+ K FN L L +I EH++ D + DF+ +Y+L + + I+ ++ +++ ++ D+ A E + + LL YP++ ++ EI++ + + +S MPYT+A++ E+QR+ + H+ DI Y IPK + ++ +L ++ HD K++ NP++ DP +FL+ +G +FK+ F+P+ G R C G + LF+++ ++ N+K+
Sbjct: 28 KLPPGPTPLPIIGNFL---QIDVKNISQSFTNFSKAYGPVFTLYLGSKPTVILHGYEAVKEALIDR-GEEFAGRGSFPMAEKIIKGFGVVFSN---GNRWKEMRRFTLMTLRNLGMGKR--NIEDRVQEEAQCLVEELRKTKGSPCDPTFILSCAPCNVICSIIFQNRFDYKD-KEFLILMDKINENVKILSSPWLQVCNSFPSL-IDYCPGSHHKIVKNFNYLKSYLLEKIKEHKESLDVT----NPRDFI-----DYYLIKQKQVNHIEQSEFSLENLASTINDLFGAGTETTSTTLRYALLLLLKYPDVTAKVQEEIDRVVGRHRSPC-MQDRSHMPYTDAMIHEVQRFIDLLPTSLPHAVTCDIKFRKYLIPKGTTVITSLSSVLHDSKEFPNPEMFDPGHFLNGNG-NFKKSDYFMPFSTGKRICAGEGLARMELFLILTTILQNFKL 458
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. UniProt/SwissProt
Match: sp|P12394|CP17A_CHICK (Steroid 17-alpha-hydroxylase/17,20 lyase OS=Gallus gallus OX=9031 GN=CYP17A1 PE=2 SV=1) HSP 1 Score: 142.51 bits (358), Expect = 1.930e-35 Identity = 108/450 (24.00%), Postives = 211/450 (46.89%), Query Frame = 3
Query: 204 KYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLD-RLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIF--NDVDYKKY----QGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSK---KLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHW----KSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKR-RKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEPFNIQ 1508
+YG + L+ VV +N + EV ++K K +GR + +LS +I + YGPLWK ++ +H L L +I+ + ET+ +++ +++ +L NV+ L FN + D +++ QG ++ + + + FP + D +++ KV +QL LQ + EH++ C D ++ A+ + L+ ++ + G++LTD H+ ++ D+ A E W L YP +Q+ I+ E+ D + + H + +PY A + E R H ++ D +IG Y IPK + +V+NL+++HHD+K+W P+ +P FL E G+ ++P+G G+R C+G ++ LF+ + V+ + + +++ ++ K V ++ V+ F ++
Sbjct: 64 RYGSLYGLWMGSHYVVVVNSYQHAREVLLKK-GKAFAGRPRTVTTDLLSRGGKDIAFASYGPLWKFQRKLVHAALSMFGEGSVALEKIICREAASLCETLG---AAQDMALDMAPELTRAVTNVVCSLCFNSSYRRGDPEFEAMLEYSQGIVDTVAKESLVDIFPWLQIFPNRDLALLKRCLKVRDQL---LQQKFTEHKEA-----FCGDTVRDLMDALLQVRLNAENNSPLEPGLELTDDHLLMTVGDIFGAGVETTTTVLKWAVLYLLHYPEVQKKIQEEM-----DQKIGLARHPHLSDRPLLPYLEATISEGLRIRPVSPLLIPHVSLADTSIGEYSIPKGARVVINLWSVHHDEKEWDKPEEFNPGRFLDEQGQHIHSPSPSYLPFGAGIRVCLGEVLAKMELFLFLAWVLQRFTLECPQDQPLPSLEGKFGVVLQ-VQKFRVK 495
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. UniProt/SwissProt
Match: sp|Q92113|CP17A_SQUAC (Steroid 17-alpha-hydroxylase/17,20 lyase OS=Squalus acanthias OX=7797 GN=CYP17A1 PE=2 SV=1) HSP 1 Score: 134.035 bits (336), Expect = 2.096e-32 Identity = 114/431 (26.45%), Postives = 191/431 (44.32%), Query Frame = 3
Query: 183 IVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVD-----YKKY-QGAIEVIPQ------YAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNV--EVT--LHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSF-KRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKM 1424
+ QK YG + +L V IN+ + EV ++K K +GR + +LS +I YGP WK ++ + L + E M C + + R N ++ D+ NVI +L FN + D +KY QG + + + + FPN+ ++ L++ +LQ + ++H K +Y + +LL + + S LTD H+ ++ D+ A E +F W+ L +P +Q+ I+ EI D+N+ E T + K M + NA + EI R H + D +IG Y IPK + +++NL+AIHHD+K+WKNPD DP FL EDG+ + ++P+G G R C+G + LF+ ++ + +
Sbjct: 57 LFQKLQKTYGNLFSLMMGPHYAVVINNHQHAKEVLLKK-GKIFAGRPSMVTTDLLSRGGKDIAFGKYGPAWKFHRKLVLSALHLFGDGSAGIEKMI-CQEATSMCSTFER-LNNAAHDMMPDVTRAVTNVICLLCFNSTYEKEDPEFQTMRKYSQGIVNTVAKDSLIDIFPWLQFFPNE---------NLHTLKQCIATRDSILQKKFEDH--KANYSSDSANDLFNILLKAKMNAENNNSSVHEAGLTDDHMVMTVADIFGAGVETSSTAFAWMIIYLIHHPEVQKKIQEEI-----DSNIGFERTPKMSDKGNMNFLNATIHEILRIQPVSPLLIPHVALADSSIGDYTIPKGTRVIINLWAIHHDEKEWKNPDAFDPGRFLDEDGKYVCSSSESYLPFGAGTRVCLGEMLARMELFLFTSWILQRFTV 468
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. UniProt/SwissProt
Match: sp|P70085|CP17A_ORYLA (Steroid 17-alpha-hydroxylase/17,20 lyase OS=Oryzias latipes OX=8090 GN=cyp17a1 PE=2 SV=1) HSP 1 Score: 132.109 bits (331), Expect = 8.989e-32 Identity = 105/458 (22.93%), Postives = 208/458 (45.41%), Query Frame = 3
Query: 168 LIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGL----RHCANLDRLNEIMYKCFDKFKETVEYRRGSKNG-GIEVDNDLFWMADNVITMLLFNEIFNDVD------YKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIAC-FDITDFVLLA---VEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFK-RRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVE 1493
+++K++ QKY G +L V+ +N EV +++ + +GR + +L+ + +I DY W+ ++ +H L A+L R+ C + + G +++ +L NVI L FN ++ D + QG ++ + + + FP + D + +++ V +QL LQ + +EH K DY D+ D +L A E + + + L+D H+ ++ D+ A E W L YP +Q+ I+ E+++ + + L + +P+ A + E+ R H + D ++G + + K + +V+NL+++HHD+K+W NPD+ +P FLS DG S ++P+G GLR C+G ++ LF+ + ++ + + ++S ++ K V ++ V+
Sbjct: 56 VLFKELQQKY----GQTYSLKMGSHQVIIVNHHAHAREVLLKR-GRTFAGRPRTVTTDVLTRDGKDIAFGDYSATWRFHRKIVHGALCMFGEGSASLQRI-----ICTEAQSLCSTLSEAAATGLALDLSPELTRAVTNVICSLCFNSSYSRGDPEFEAMLRYSQGIVDTVAKDSLVDIFPWLQIFPNKDLRLLKQCVAVRDQL---LQKKFEEH--KSDYSDHVQRDLLDALLRAKRSAENNNTAAEFSAEAVGLSDDHLLMTVGDIFGAGVETTTTVLKWAITYLIHYPEVQKQIQEELDR-KVGVDRPPQLSDRGSLPFLEATIREVLRIRPVAPLLIPHVALSDTSLGDFTVRKGTRVVINLWSLHHDEKEWTNPDLFNPGRFLSADGSSLTLPSSSYLPFGAGLRVCLGEALAKMELFLFLSWILQRFTLSVPPSQSLPSLEGKFGVVLQPVK 497
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. UniProt/SwissProt
Match: sp|O57525|CP17A_RANDY (Steroid 17-alpha-hydroxylase/17,20 lyase OS=Rana dybowskii OX=71582 GN=CYP17A1 PE=2 SV=1) HSP 1 Score: 130.568 bits (327), Expect = 3.325e-31 Identity = 106/443 (23.93%), Postives = 196/443 (44.24%), Query Frame = 3
Query: 204 KYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECDFPNDKPSKKSDF--------KSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDG-RSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEPFNI 1505
KYG + + VV +N+ E EV ++K K GR + IL+ + +I +DY P WK ++ +H L C + I + + + +++ +L NV+ L FN YK+ E + +Y++ K S F K ++ L++ ++LQ +I+EH K D+ D +L A + + + LT+ H+ ++ D+ A E W L YP +Q+ I+ E++ + L + + YT A + E+ R H + + +IG Y IPK++ +V+NL+++HHD+K+W NP + P FL E+G R + F+P+G G+R C+G ++ +F+ + ++ + + + ++ K V I+ V+PF +
Sbjct: 73 KYGSLYSFMMGSHYVVVVNNHEDAREVLLKK-GKTFGGRPRTVTTDILTRDGKDIAFADYSPTWKFHRKMVHSAL--CMFGEGTVAIEQIISREAASLCQTLTSFQRIPLDMAPELIRAVTNVVCSLCFN-----TRYKRGDAEFETMLKYSKGIVDTVAKDSLVDIFPWLQIFPNKDLDILRQSVAARDQLLQKKINEH-KDAFCGETVKDLVDALLKAKLNMENNNSNVSQDVGLTEDHILMTVGDIFGAGVETTSTVLKWAVAYLLHYPEVQKKIQEELD-VKVGFGRYPLLSDRKILHYTEAAISEVLRIRPVSPLLIPHVALKESSIGEYTIPKEARVVINLWSLHHDEKEWVNPHLFSPDRFLDENGNRVYSPSPSFLPFGAGIRVCLGEALAKMEVFLFLSWILQRFTLEVPEGDPLPDLEGKFGVVIQ-VKPFKV 504
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. UniProt/SwissProt
Match: sp|P30437|CP17A_ONCMY (Steroid 17-alpha-hydroxylase/17,20 lyase OS=Oncorhynchus mykiss OX=8022 GN=cyp17a1 PE=2 SV=1) HSP 1 Score: 129.798 bits (325), Expect = 5.385e-31 Identity = 102/453 (22.52%), Postives = 209/453 (46.14%), Query Frame = 3
Query: 183 IVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGL----RHCANLDRL--NEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIF--NDVDYKKY----QGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYK-IACFDITDFVLLA--VEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFK-RRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVE 1493
+ QK KYG +L V+ +N + EV ++K K +GR + +L+ + +I +DYG W+ ++ +H L A+++++ E + C D +E S + +++ +L NV+ L F+ + D +++ QG ++ + + + FP + +D + +++ + ++L LQ + +EH K DY D+ D +L A E + + + L++ H+ ++ D+ A E W L +P +Q+ I+ E++ + L + +PY A + E+ R H D +IG + + K + I++NL+++HHD+K+WKNP++ DP FL+E+G ++P+G G+R C+G ++ +F+ + ++ M + ++ K V ++ V+
Sbjct: 58 LFQKLQQKYGHTYSLMMGPHTVILVNHHQHAKEVLLKK-GKIFAGRPRTVTTDLLTRDGKDIAFADYGATWRFHRKTVHGALCMFGEGSASIEKIICREALSLC-DTLRE-------SGSASLDLSPELTRAVTNVVCSLCFSSSYCRGDPEFEAMLQFSQGIVDTVAKDSLVDIFPWLQVFPNADLRLLKQCVSIRDKL---LQKKYEEH--KSDYSDHEQRDLLDALLRAKRSAENNNTAEITMETVGLSEDHLLMTVGDIFGAGVETTSTVLKWAIAYLIHHPQVQQRIQEELDSVVGGDRTP-QLSDRGSLPYLEATIREVLRIRPVAPLLIPHVAQTDTSIGKFTVRKGARIIINLWSLHHDEKEWKNPEMFDPGRFLNEEGTGLCIPSPSYLPFGAGVRVCLGEALAKMEIFLFLSWILQRLTMTVSPGQPLPSLEGKFGVVLQPVK 495
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. TrEMBL
Match: C3ZZH5 (Uncharacterized protein (Fragment) OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_145601 PE=3 SV=1) HSP 1 Score: 163.696 bits (413), Expect = 3.245e-41 Identity = 114/417 (27.34%), Postives = 201/417 (48.20%), Query Frame = 3
Query: 207 YGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFN--DVDY----KKYQGAIEVIPQYAEECDFPNDK--PSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIK----LTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYK 1421
YG + +L T VV +N ++ E FV+K + +GR I S +I +DY P WK ++ H +R A+ L K + ++T+ + +++++ ++ + NVI F +N D D+ K + E Q +P + PS SV +K+ +QL+E++Q +++HR+ D DITD ++ A ++ +++ GI+ LTD H++ + D+ FA + W LA++P IQE + E++ + + L + PYT A+ E+ R AS D + H+T D + Y+IPK + I+ NL+A+HHD W +PD+ P+ FL E G+ + +P+G+G R C G + F+L+ +V N++
Sbjct: 11 YGDVFSLKMGMTDVVVLNSLDAVKEAFVKK-GEDFAGRPKMLSTDISSEGGKDIAFADYSPTWKLHRKLFHSAIRGYASAQNLQS---KVHESLEDTIAVFSKMEGQAVDLEDYIYQLVYNVICSAAFGTRYNMDDEDFNTLMKISKDTTETFGQGLLADVYPLLRFLPSS-----SVTANRKMTHQLMEIMQRHLEQHRESFDPN-NLRDITDHMIKAQKD------AEEEGIQDIDSLTDTHLRQLIGDIFFAGTISTILTLRWAILYLAVHPEIQEKVAAELDSVVGRDRLP-ELSDREATPYTEAIFHEVMRMASMDPVSLPHATTVDTTLRGYQIPKGTWILPNLWALHHDPDTWGDPDVFRPERFLDESGKPIPKPAALMPFGVGRRACPGEALGKADTFLLLGGLVQNFR 410
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. TrEMBL
Match: C3YIB9 (Uncharacterized protein (Fragment) OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_211258 PE=3 SV=1) HSP 1 Score: 161.77 bits (408), Expect = 2.937e-40 Identity = 116/451 (25.72%), Postives = 212/451 (47.01%), Query Frame = 3
Query: 96 ISSYKLPPGPNRNSDEADFIINEKLIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFN--DVDYKKYQGAIEVIPQYAEECDFPNDKPSKK---SDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIK----LTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYK 1421
+ +++PPGP + N ++ I KYG + +L T VV +N ++ E FV+K +GR +IL+ +I +DY P WK L R L G+ H + K + ++T+ + ++++ ++ + NVI F +N D D+ + + + + + PS + S +S+ +K+ Q + +LQ ++EHR+ D DITD ++ A ++ +++ GI+ LTD H++ ++ DV + + W LA +P IQE + E++ + + + + MPYT A + EI R S + H+T D + Y+IPK + I+ NL+A+HHD W +PD+ P+ FL EDG+ + +P+ G R C G+ ++ V ++L+ +V N++
Sbjct: 20 MKRWRMPPGPFFWPIIGNIYCNGRMYITFI--DLAQKYGDVFSLKMGMTDVVVLNSLDAVKEAFVKK-GVDFAGRPKILSNEILTEGGKDIAFTDYSPTWK-LHRKLFHGVTHWGQ-----NLQTKVHESLEDTIAEFSKMEGQAVDLEGYIYKLVYNVICSTAFGVRYNMDDEDFITLMKISKDMTETLGQGLLADVYPSLRFLPSPGESLTAFRKMTEQSLTILQRHLEEHRETFDPS-NLRDITDHMIKAQKD------AEEEGIQDINSLTDTHLRQTMNDVFAGGTDTTIWTLRWANLYLAAHPEIQEKVAAELDSVVGRDRLP-EMSDREAMPYTEATIHEIMRMGSIAPLSLPHATTVDTTLSGYQIPKGTWIMPNLWALHHDPDTWGDPDVFRPERFLDEDGKPIPKPAALMPFSAGRRACPGDAVAKAVTYLLLGGLVQNFR 453
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. TrEMBL
Match: A0A1S3I374 (cytochrome P450 1A1 OS=Lingula unguis OX=7574 GN=LOC106160616 PE=3 SV=1) HSP 1 Score: 159.073 bits (401), Expect = 8.323e-39 Identity = 116/444 (26.13%), Postives = 219/444 (49.32%), Query Frame = 3
Query: 105 YKLPPGPNRNSDEADFII--NEKLIYKKIVQKYIPKYGPICTL-YKDGTPVVFINDIETGYEVFVRKLSKQVSGRSA-SYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIF--NDVDYKKYQGAIEVIPQYAEECDFPND--KPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKR--RKDFVPYGLGLRTCVGNTFSE-NVLFVLIML 1403
YKLPPGP + + N+ +Y + K +G + ++ VV + + +E V K + +GR ++ ++ SG + +++ SDYGP+W+ ++ K R D+L FD+ +E + + + +F N++ + F +++ N+ ++ +G ++ + P+D K S +++ LI+ ++++++H++ + A DITD +LL EE + + + L+DL + H+++D+ FA + ++ +W+ A PNIQE I E++ DD +SK+PY AV E+ R A + H + D +G Y+IPK++LI N++AIHHD K W++P++ P+ FL +DG S K +K ++P+ G R CVG + N++ +LI+
Sbjct: 34 YKLPPGPPQLPILGSYFAFRNDGRLYS-VFSKLGKSFGDLFSVDLGFWHTVVVLKSADIVHEALVEK-KETFAGRGVLTWKYELFSGRKRDLIFSDYGPVWRLQKQMALKAFRTYVASDKLEMFTRSAFDEIAALIEKETEPFDMHVYMRLLIF----NIVCRMAFGKVYKINEPEFVWLKGTLDESMEKVYGSIIPSDVIPVLKHLPIPSSLSTKRLLQGLIDFQKVKLEDHKRTINLGEAR-DITDQMLLLREELTAE-RDDDVKESLSDLRILHTILDIFFAGTQTTSDTLIWMILYAADNPNIQEKIHEELDSLGDDFCD--LRQCRSKLPYCEAVQREVLRMRPAGPVSIPHRALCDTKLGGYDIPKNTLIFANIWAIHHDPKNWESPEVFMPERFLEKDG-SLKELDKKTWLPFSSGKRKCVGEAIARTNIMTILILF 466
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. TrEMBL
Match: C3YIB7 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_73418 PE=3 SV=1) HSP 1 Score: 156.377 bits (394), Expect = 5.727e-38 Identity = 112/426 (26.29%), Postives = 205/426 (48.12%), Query Frame = 3
Query: 207 YGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECD-------FPNDK--PSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIK----LTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKS 1445
YG + +L T VV +N ++ E F++K +GR I S +I+ +DY P WK ++ H +R A+ L K + K+T+ + G+ +D+ L+ + NVI F +N +D + ++ ++V AE +P + PS +V +K+ +Q++ ++Q +++H++ D DITD ++ A ++ +++ GI+ LTD H++ ++ D+ F S W LA++P IQE + E++ + + L + PY A + E+ R AS D + H+T D + Y+IPK ++++ NL+A+HHD W +PD+ P+ FL E+G+ K +P+ G R C G T + F+L+ +V N++ K +
Sbjct: 53 YGDVFSLKLGMTDVVVLNSLDAVKEAFMKK-GADFAGRPKMLSMDISSEGGKDIVFTDYNPTWKLHRKLFHGAIRGYASGQNLQS---KVHESLKDTIAEFTKMEGQGVNLDDYLYQLVYNVICSAAFGVRYN-MDDENFKTLMKVSKDAAERFGQGFLADVYPLLRFLPSP-----TVTACRKMMDQILGIMQCHLEQHKETFDPSNR-RDITDHMIKAQKD------AEEEGIQDINSLTDTHLRQTISDIFFGGTFSSIFSLRWALLYLAVHPEIQEKVAAELDSVVGRDRLP-ELSDREATPYMEATIHEVMRMASMDPLSLPHATTVDTTLRGYQIPKGTMVMPNLWALHHDPDTWGDPDVFRPERFLDENGKPVKPAA-LMPFSAGRRACPGETLGKANNFLLLGGLVQNFRFSISKGEG 459
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. TrEMBL
Match: A0A210R6Z5 (Steroid 17-alpha-hydroxylase/17,20 lyase OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT16032 PE=3 SV=1) HSP 1 Score: 154.066 bits (388), Expect = 6.886e-37 Identity = 113/461 (24.51%), Postives = 223/461 (48.37%), Query Frame = 3
Query: 75 IITMNRTISSYKLPPGPNRNSDEADFIINEKLIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIE-VDNDLFWMADNVITMLLFNEI--FNDVDYKKYQGAIEVIPQYAEECDF---PNDKP--SKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRK---KGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKM 1424
+ + R I+ K PPGP ++ + + + KYGP+ T+ + V +N IET E V++ + +GR LS +IL S Y P K ++ + LR L I+ K +F + E +G+ E +D LF N+I + F ++ F+D +K Y +++ Q+ E + P +K + +K+ +F +L+ + ++ H++ +G+ + D D +L+A +E + + L +LTD+H++ +L D A+ + + W +A + ++Q ++ E+++ + + + + +PYT A L E+ R AS H TM D ++G Y +PK + +++N +A+H+D + WK P +DP+ FL+ DG ++ + F+P+ GLR C+G + ++ L +++ L++ + +
Sbjct: 37 VFILCRLITKPKYPPGPMALPVIGNYKLLTDPKIHEACARLSEKYGPVVTVQFGPSKTVVLNSIETVVEAMVKRRA-DFAGRPPVPSGMTLSEGGKDILMSQYTPSLKVHRKIVSAALRKYIKGPFLQTILEKSMGQFIKLFEETKGNPFIPHEYIDLALF----NIICGVCFQKVYKFDDPKFKHY---LDIDNQFVAEFGMGLIEDLYPILAKVWQSQRYKKVVSLFRELLHYIHEELEAHKQDFDEGNIR----DFADCLLMARKEAENEEDVEILS-QLTDVHLRQTLSDTFTAAFDTTRFTIQWALLYMADHQDVQRKVQAELDRVVGRDRLP-RVDDRPSLPYTEATLFEVMRIASVAPLGMPHMTMVDSSVGGYTVPKGTTVIINHWALHNDPRHWKEPSKLDPERFLNSDGTLARKPESFLPFSAGLRVCLGESTAKPELVIILALILHQFNI 483
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Cavefish
Match: cyp17a2 (cytochrome P450, family 17, subfamily A, polypeptide 2 [Source:ZFIN;Acc:ZDB-GENE-100822-1]) HSP 1 Score: 130.568 bits (327), Expect = 2.484e-32 Identity = 94/441 (21.32%), Postives = 190/441 (43.08%), Query Frame = 3
Query: 204 KYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCA-NLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQ------GAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHR---KKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIK-LTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVE 1493
+YGP+ +L + ++++ EV + + + +GR +++ +I +DY PLWK +R +H +L I+ + ++ RG +++ L NV+ L+FN + D + Q G ++ I + FP + D + + + ++L L +++EH+ G+ + D+ D +L+ +S G +TD HV + + A E + +W L +P +Q+ + E+++ + + L +S +P+ ++ LCE+ R H M D ++G + +PK + +++N++AIHHD K W+ PD P FL G+ F+P+G G R CVG + + LF+ + ++ + S + + V ++ +
Sbjct: 69 RYGPLFSLRAGRNYTLVVSEVGLAREVLLHR-GRDFAGRPQMVTTALMTRGGKDIAFADYSPLWKKHRRLVHSSFTLFGEGSSKLQNIVLDAAESLCSDLQACRGQH---VDLCPMLMRAVTNVVCRLVFNSSYRPHDPELQQVMDYNDGIVQTIARGGLVDIFPWLQIFPNKDLAKLRECVSIRDKL---LTKKLEEHKLTYTPGEPR----DLLDALLIG--------QSGSAGEDDITDDHVLMTAAEAFGAGVETTSTTLLWTIAFLLHHPQVQDRLHAELDE-QVGRDRPPALSDRSNLPFLDSALCEVMRIRPVSPILIPHVAMQDTSLGGHAVPKGTRVLVNMWAIHHDPKHWEEPDQFRPDRFLDATGKKIA-PPSFLPFGAGPRVCVGESLARMELFLFVSCLLQRFHFSVPAGASLPDLQGRYGVVLQPLR 488
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Cavefish
Match: ENSAMXT00000044947.1 (pep primary_assembly:Astyanax_mexicanus-2.0:11:11445979:11463125:-1 gene:ENSAMXG00000016265.2 transcript:ENSAMXT00000044947.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 130.568 bits (327), Expect = 4.037e-32 Identity = 105/443 (23.70%), Postives = 186/443 (41.99%), Query Frame = 3
Query: 204 KYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIF--NDVDYKKYQGAIEVIPQYAEEC------DFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKN-AVFIRDVEPFNI 1505
+YG I LY T +V +N E E V+K S +GR +SY +SG NI DY +WK +R +H L+ C + I + + ++YR + +++ D A NVIT L F + + + + +K G + I FP + F L K + E++ +DE +++ K + ++E K K L LTD + + ID++ E W L P +Q + E+ + + + ++PY AV+ E+ R H+ D +I Y IPK+++I+ NL+ HHD W P P+ FL G S + +P+G G R C+G + ++ LF+ ++ +++ + + +E+ D + A + +P+ +
Sbjct: 93 RYGNIYRLYCGSTTMVILNSSEFIREALVKKWS-DFAGRPSSYTGDKVSGGGQNISLGDYSEVWKAQRRVVHNALQRCTAESLHSVIETQALHLKQVLLDYREKA----VDLSEDFTVAASNVITTLAFAKSYDKSSAELQKLHGCLNKIVSLWGSLWISVLDSFPLLRRLPNPHF---SLLMKEVARRDEIIVGLLDEFKQRSGAKDGGTLTASLLQHSLESADKTAKPKTL---LTDTQLHMTTIDLLIGGTETTAAWLCWTVAFLLHRPEVQTGVYEELCSVLKGQYPQYSD--RHRLPYLCAVINEVLRLRPVAPLAVPHTAKRDSSIAGYFIPKNTVIIPNLFGAHHDPAVWSEPFTFRPERFLEGGGGSL---RALMPFGGGARLCLGESVAKMELFLFTAYLLRDFQFL--PEQPEELPDLRGVATVVLKAKPYRV 517
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Cavefish
Match: cyp17a1 (steroid 17-alpha-hydroxylase/17,20 lyase [Source:NCBI gene;Acc:103036160]) HSP 1 Score: 126.331 bits (316), Expect = 1.145e-30 Identity = 96/420 (22.86%), Postives = 193/420 (45.95%), Query Frame = 3
Query: 189 QKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIF--NDVDYKKY----QGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEI-NKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRK-DFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKM 1424
Q+ KYG I +L V+ +N EV ++K K +GR+ + IL+ + +I +DY W+ ++ +H L C + I + + +N +++ +L NV+ L F+ + D +++ QG ++ + + + FP + D + + + + ++L LQ + +EH+ + D+ D +L A + S + + LT+ H+ ++ D+ A E W L +P +Q+ I+ E+ +K D + + L + +PY A + E+ R H + D +IG Y + K + +++NL+++HHD+K+WKNP++ DP FL E+G ++P+G G+R C+G ++ LF+ + ++ + +
Sbjct: 62 QQLQKKYGDIYSLMMGSHKVLIVNSHHHAKEVLLKK-GKIFAGRARTVTTDILTRDGKDIAFADYSATWRFHRKMVHGAL--CMFGEGSASIEKIICREASSMCKVLTDLQNSAVDMAPELTRAVTNVVCSLCFSSAYKRGDAEFEAMLQYSQGILDTVAKDSLVDIFPWLQFFPNEDLRILRQCVSIRDKL---LQKKYEEHKANYSDNVQ-RDLLDALLRAKSSSENNNTSTQ-DVGLTEDHLLMTVGDIFGAGVETTTTVLKWSIAYLIHHPQVQKKIQEELDSKIGRDRHPQ--LSDRGNLPYLEATIREVLRIRPVSPLLIPHVALSDSSIGEYTVQKGTRVIVNLWSLHHDEKEWKNPELFDPGRFLDEEGNGLCNPSLSYLPFGAGIRVCLGEALAKMELFLFLSWILQRFTL 471
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Cavefish
Match: ENSAMXT00000016766.2 (pep primary_assembly:Astyanax_mexicanus-2.0:11:11445979:11462938:-1 gene:ENSAMXG00000016265.2 transcript:ENSAMXT00000016766.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 124.79 bits (312), Expect = 3.291e-30 Identity = 103/449 (22.94%), Postives = 186/449 (41.43%), Query Frame = 3
Query: 204 KYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIF--NDVDYKKYQGA------------IEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKN-AVFIRDVEPFNI 1505
+YG I LY T +V +N E E V+K S +GR +SY +SG NI DY +WK +R +H L+ C + I + + ++YR + +++ D A NVIT L F + + + + +K G I V+ + PN P K V + ++ L++ +++I+ + + VLL+ LTD + + ID++ E W L P +Q + E+ + + + ++PY AV+ E+ R H+ D +I Y IPK+++I+ NL+ HHD W P P+ FL G S + +P+G G R C+G + ++ LF+ ++ +++ + + +E+ D + A + +P+ +
Sbjct: 82 RYGNIYRLYCGSTTMVILNSSEFIREALVKKWS-DFAGRPSSYTGDKVSGGGQNISLGDYSEVWKAQRRVVHNALQRCTAESLHSVIETQALHLKQVLLDYREKA----VDLSEDFTVAASNVITTLAFAKSYDKSSAELQKLHGCLNKIVSLWGSLWISVLDSFPLLRRLPN--PHFSLLMKEVARRDEIIVGLLDEFKVKIE---------MLIMSVALSVLLSQ--------------LLTDTQLHMTTIDLLIGGTETTAAWLCWTVAFLLHRPEVQTGVYEELCSVLKGQYPQYSD--RHRLPYLCAVINEVLRLRPVAPLAVPHTAKRDSSIAGYFIPKNTVIIPNLFGAHHDPAVWSEPFTFRPERFLEGGGGSL---RALMPFGGGARLCLGESVAKMELFLFTAYLLRDFQFL--PEQPEELPDLRGVATVVLKAKPYRV 493
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Cavefish
Match: ENSAMXT00000055532.1 (cytochrome P450 2J2-like [Source:NCBI gene;Acc:103031006]) HSP 1 Score: 124.02 bits (310), Expect = 5.807e-30 Identity = 120/472 (25.42%), Postives = 197/472 (41.74%), Query Frame = 3
Query: 99 SSYKLPPGPNRNSDEADFIINEKLIYKKIVQ--KYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSK-----NGGIEVDNDLFW-------MADNVITMLLFNEIFNDVDYKKYQGAIEV-----------IPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLH---VKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVW 1430
S + PPGP F+ N I K + K +PKYG +C++Y P + +N ++ E FV+ + S K G I+ + YG WK +R LR F K+TVE R + ++ + F+ N+I ++F + F + D K++ +E+ I Q F P +K+ K N L E + +EHRK D + ++ DF+ + +L K+ + + H + S D+ A E N+ W + +P++QE EI + + ++ ++++PYT+A + EIQR A+ H T + Y IP+ + I+ NL AI DK WK PD +P+NFL E G+ F + F+P+ LG R C+G T + LF+ ++ K W
Sbjct: 35 SRSRSPPGPK----PMLFVGNLPQIMKDRMAFIKMMPKYGEMCSMYLGKKPTIVLNTVQIAKEAFVQNGTVFSSRPDNRLIRKFTQG--CGIIMAPYGNSWKQQRRFALHTLRD--------------FGLGKKTVEERVAEEARYLIKEMLKQEGKAFYPIHPIMNAVSNIICSIVFGDRF-EYDDKQFAKLLEILNENVRFTGSFISQTYLVIPFVEHLPGPH------QKVWKNLNSLREFILGVAEEHRKTLDPE----NLRDFI-----DAYLVEMIKQESNEDSTFHEENMAKSTADLFSAGTETTANTLRWGLIYMMEHPDVQERCHEEIVRVLGFDRFP-SMDDRAQLPYTHATVHEIQRNANIIPLGVMHHTTQPTTLRGYHIPQGTDIMSNLTAILTDKDHWKYPDTFNPENFLDEKGQ-FCKNDSFLPFSLGPRVCLGETLARTELFIFFTSLIQRLKFSW 468
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Sea Lamprey
Match: cyp17a1 (cytochrome P450, family 17, subfamily A, polypeptide 1 [Source:ZFIN;Acc:ZDB-GENE-040213-2]) HSP 1 Score: 127.102 bits (318), Expect = 1.256e-31 Identity = 104/423 (24.59%), Postives = 182/423 (43.03%), Query Frame = 3
Query: 207 YGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYK------------KYQG--AIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKD---FVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKM 1424
YG + L G +V +N++E+ E+ ++K S +GR +Y ++G +I DY +W+ +R L HC R EI+ + E + G ++ D+ NVI + F ++ D + + G AI + + FPN P+ + +V K EV+ +I H+ K + D+TDF+L + +S+ G LT+ V S++D A E W L L+P +Q+ I E+ + + + +L + K+ Y +A + EI R H + D ++G + I K S ++ NL+A H D W NP + P FL G S +RR +P+G G R C+G + ++ +F+ + V+ ++
Sbjct: 75 YGAVYCLRVGGEDIVVLNNVESIKEMLIKK-SADFAGRPQTYAGGKITGGGKDIALGDYSAVWRRQRRAALSAL-HCVGTARTEEIVQGEAQRLVEDLTQYIGRPT---DIHTDISMRVCNVICSVAFGTKYDKHDTEFLDIHCCLDSLVRLWGSPAIRALDCFPVLRLFPN--PAWRDLMAAVRKRD-------EVVWRQILLHKAKPSSSPS-SDMTDFLLKFTRHSRNEEESEARGGILTEERVHLSIMDTFIAGVETTATLIEWALAYLVLHPEVQDQIYEEMEQ-KLQPGLGPSLADRDKLLYLDATIKEILRLCPVAPLAIPHRALRDSSVGGFFICKGSTVIANLWAAHRDHAHWDNPSVFQPGRFL---GESLERRNPASLLLPFGAGKRVCLGESLAKAEIFLFLGHVLRRLRL 478
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Sea Lamprey
Match: cyp1d1 (cytochrome P450, family 1, subfamily D, polypeptide 1 [Source:ZFIN;Acc:ZDB-GENE-041114-179]) HSP 1 Score: 110.153 bits (274), Expect = 6.674e-26 Identity = 98/448 (21.88%), Postives = 193/448 (43.08%), Query Frame = 3
Query: 204 KYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRH-CANLDRLNEIMYKCFDKFKETVEYRR--------GSKNGGIEVDNDLFWMADNVITMLLFNEIF--NDVDYKKYQGAIEVIPQYAEECD----FPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHR---KKGDYKIACFDITD-FVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRR--KDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIR 1484
+YG + ++ PVV ++ +ET V+ +GR Y ++ Y WK +R LR+ CA R Y C + E R G++ GG + + NV+ L+F + + D + + G E Q + + FP + + ++++ L +L+ ++ I EH +KG + DITD + + E+ H +++G L+D V + +D+ A + + W L ++P++Q ++ EI++ + ++ + A L E+ R+++ FT HST D+ + IP+D+ + +NL+ ++HD W +P+ DP FLS DG+ + + +GLG R C+G+ + LFV+++ ++ + ++A+ ++
Sbjct: 87 RYGDVFRVHLGSVPVVVLSGLETIKRALVKN-GDAFAGRPPFYSLSLVGNGDTLTFTRAYDGCWKAHKRLARSALRNFCAAPARREG--YACLLEECVCEEAERLLLACKGGGAQPGGFDPVPSITCAVANVMCALVFGKRYPHGDPTFLQIVGFNEEASQLSGAGNLVDFFPFLRHVPNAQLRALKVLSA---RLLAFMEGHIKEHYSSYQKGHVR----DITDALIAMCAEQEH---ALREVG--LSDERVVATALDIFGAGFDTIITGLTWCLLYLCIHPDVQHKLQREIDETIGRDRTP-RFEDRANLRRVEAFLTEMFRHSAFVPFTIPHSTTQDVVLNGMFIPRDTCVFVNLHQVNHDPTVWSDPERFDPDRFLSHDGKEVNKEMVEKVQIFGLGKRRCLGDMVARQELFVVLVTLLQGLSFRVPPGTPTPSVAPQHALVMK 518
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000011254.1 (pep scaffold:Pmarinus_7.0:GL479221:5463:6953:-1 gene:ENSPMAG00000010227.1 transcript:ENSPMAT00000011254.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 109.383 bits (272), Expect = 9.197e-26 Identity = 105/445 (23.60%), Postives = 192/445 (43.15%), Query Frame = 3
Query: 186 VQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRH-----CANLDRLN-EIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFN--DVDYKKYQGAIEVIPQYAEE-----CD-FPN----DKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDK 1466
+Q+ KYG +CT+ TPVV ++ E EV V + + + R + L+ N L + YG WK +R LR+ D+++ E Y ET + + + + ++N L N+I LL ++ + ++K +E + Q E C+ FP P +K F KLQ + N ++ + D+H + D+ D L +++ +P S T + LI++ E N+ W + + P+IQ EI++ +S +PY +AV+ EIQR+ + H+T D+ Y PK + +++NL + HD WK P+ +P +FL +DG+ + F+P+ G R C+G + ++ +F+ ++ +++ W + S + K
Sbjct: 63 MQELAKKYGEVCTIMLGSTPVVLVSGYEAVKEVLVNR-GHEFADRPITPIFTKLTQNIGIAL-APYGDPWKQQRRFALSTLRNFGFGKATIADKISIEAKY-----LIETFKQETSAFDPHLNLNNAL----GNIICCLLNRNRYDYDNEQFQKLTHLLEYLVQLQAEPLVILCNVFPFLLKLPGPWQKV-FHIHSKLQCLLNDIVSEHRATKDQHNPR--------DLIDSYLAQIDKEKDNPNSS-----FTHQSLIQILIELFIGGLETTSNTLRWGLLFMMMNPDIQARCHGEIDRVVGSERPPCMTDRRS-LPYLDAVIHEIQRFGNVLPLGVLHATSKDVQFNDYLFPKGTHVMVNLMSALHDDIHWKTPNKFNPDHFLDKDGQCIQ-SDAFLPFSAGPRVCLGESLAKMEIFLFFTSLLQHFEFYWPNSVSPPTLAAK 480
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002462.1 (pep scaffold:Pmarinus_7.0:GL485824:2571:10389:-1 gene:ENSPMAG00000002249.1 transcript:ENSPMAT00000002462.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 105.916 bits (263), Expect = 2.230e-25 Identity = 69/236 (29.24%), Postives = 113/236 (47.88%), Query Frame = 3
Query: 756 LQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQ-ENIRTEINKF----RDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQ 1448
Q RI EHR+ + D+ D L +E+ DP+S TD ++ + + D+ A E + W + YP IQ E + EI++ R N + ++ +PYT+AV+ E QR A+ HST D+ Y IPK + ++LNL ++ HD+ QWK P+ +P +FL G+ F + F+ + G R C+G + LF+ ++ +K W S+
Sbjct: 83 FQERIREHRQTREAGQPR-DLIDAYLDEMEKESQDPEST-----FTDGNLLNVVGDLFIAGTETSSTTLRWALLYMMAYPQIQAERCQREIDEVIGGDRSPNTTD-----RANLPYTDAVIHEAQRMANVAPLGVLHSTTTDVKFRGYNIPKGTKVLLNLTSVLHDETQWKTPNEFNPDHFLDNTGQ-FVKPDAFLAFSAGPRVCLGENLARMELFLFFTSLLQRFKFHWPDATSR 306
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Sea Lamprey
Match: cyp2u1 (cytochrome P450, family 2, subfamily U, polypeptide 1 [Source:ZFIN;Acc:ZDB-GENE-070730-1]) HSP 1 Score: 104.375 bits (259), Expect = 4.459e-25 Identity = 49/179 (27.37%), Postives = 90/179 (50.28%), Query Frame = 3
Query: 921 DVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDIN--IGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQE 1451
D+ A + N+ +W L+ YP +Q ++ E++ V+L + ++P T A + E+QR +S HS + + + IP+ S+++LNL+A H D + W P + DP+ FL GR +RR F+P+G+G R+C+G + + LF+ ++ + S +
Sbjct: 106 DLFIAGTDTTANTLLWCLLLMCTYPAVQARVQAELDAVVGRGR-RVSLSDRPRLPLTVATIMEVQRLSSVVPLAIPHSATRSVRTELRGFTIPRGSVVLLNLWACHVDPRVWARPLLFDPERFLDPHGR-LRRRDAFMPFGIGRRSCLGESLARVELFLFFSALMQEFTFTSSAPHSPD 282
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Yeast
Match: ERG5 (C-22 sterol desaturase; a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs [Source:SGD;Acc:S000004617]) HSP 1 Score: 75.0998 bits (183), Expect = 8.389e-15 Identity = 44/164 (26.83%), Postives = 84/164 (51.22%), Query Frame = 3
Query: 885 KLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRD-DNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGP-YEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTF 1370
+ T+ + ++ +FASQ+ + W+F ++A P++ IR E R+ D + E+ L KM YTN V+ E RY + + + P Y PK ++++ LY HD + ++NPD P+ ++ E ++ + +K+++ +G G C+G T+
Sbjct: 320 EFTNKEISEAVFTFLFASQDASSSLACWLFQIVADRPDVLAKIREEQLAVRNNDMSTELNLDLIEKMKYTNMVIKETLRYRPPVLMVPY-VVKKNFPVSPNYTAPKGAMLIPTLYPALHDPEVYENPDEFIPERWV-EGSKASEAKKNWLVFGCGPHVCLGQTY 481
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Yeast
Match: ERG11 (Lanosterol 14-alpha-demethylase; catalyzes C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in ergosterol biosynthesis pathway; transcriptionally down-regulated when ergosterol is in excess; member of cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p; human CYP51A1 functionally complements the lethality of the erg11 null mutation [Source:SGD;Acc:S000001049]) HSP 1 Score: 51.2174 bits (121), Expect = 2.952e-7 Identity = 42/206 (20.39%), Postives = 84/206 (40.78%), Query Frame = 3
Query: 879 GIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINI--GPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKD---------------FVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKS 1445
G+K+TD + + LI V+ Q + W+ LA P++Q+ + E + D E+T +MP N + E R + F M D+++ Y IP ++++ H + + N + + + S+ ++ ++P+G G C+G F+ L VL+ + + K + + K+
Sbjct: 296 GVKMTDQEIANLLIGVLMGGQHTSAATSAWILLHLAERPDVQQELYEEQMRVLDGGKKELTYDLLQEMPLLNQTIKETLR-MHHPLHSLFRKVMKDMHVPNTSYVIPAGYHVLVSPGYTHLRDEYFPNAHQFNIHRWNKDSASSYSVGEEVDYGFGAISKGVSSPYLPFGGGRHRCIGEHFAYCQLGVLMSIFIRTLKWHYPEGKT 500
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Nematostella
Match: EDO41284 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S507]) HSP 1 Score: 134.806 bits (338), Expect = 3.469e-34 Identity = 116/457 (25.38%), Postives = 212/457 (46.39%), Query Frame = 3
Query: 207 YGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGP--LWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEY------RRGSKNGGIEVDN--DLFWMADNVITMLLFNEIF--------NDVDYKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACF-DITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDD--NNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFH-STMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGR-SFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEPFNIQ 1508
YG I TL VF++ EV + K ++GR ++ L+ +I+ DY +W+ ++ +H GLR MY F K +E + + R+ K+ G + + + + N+I+ L + F +++ + + V + F P +S F +KL ++ ++L++ + EH K + C D+TD L A E + K K +G ++TD H+ + DV FA E + W+ L YP +Q ++T+I DD + L K +PY A + E+ R A+ H +T +++ + Y IPKD++IV+NL+AIHHD ++W NPD DP F++EDG+ ++ ++P+ G R CVG + ++ LF+++ ++ + + + + + + R +PF +
Sbjct: 60 YGDIFTLKIFNDRFVFVSSGAGAREVLMGKRMNDLAGRPQTFTFNFLTRGAKDIIFGDYSRHLVWR--RKIVHSGLR-----------MY--FPKLEENIAWEVSKLERKLMKDVGTALHSRDEFSLLITNIISSFLLGKSFEPDDPDFLTTLEFTRIGSQLPVSGSILDNFPFLVHLPISESRF--YKKLVEIRDRLLDKFFL---EH--KSTFSEDCIRDLTDAFLKAKLEVEREDK-KAIG-EVTDDHITMLMTDVYFAGLETTSTTLSWLAAYLINYPQVQTRLQTDI----DDVIGSRWPVLADKPNLPYMEATIAEVLRIATVAPMGLPHKATTNNVPLMGYNIPKDTIIVMNLWAIHHDPREWPNPDKFDPDRFINEDGKFEVPGQRSYLPFSAGRRVCVGESLAKIELFLVMTRLLQQFTLETLPGQGPPSVGEASGNITRAPKPFKFR 488
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Nematostella
Match: EDO48191 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RK60]) HSP 1 Score: 129.798 bits (325), Expect = 9.848e-33 Identity = 96/416 (23.08%), Postives = 187/416 (44.95%), Query Frame = 3
Query: 207 YGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRH-CANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECDFPNDKPSKKSDF---------KSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSF-KRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYK 1421
YG + L +V +N IE E VR+ + +GR Y ++S N +I D+GPLWK ++ H LR + L L E + D+ + + G + +D+ N+I +F + D+D ++ ++ Q+ + + + D+ K + ++++ VLQ + EH ++ + D+TD +L A + + + + +T+ H+ ++ D+ A E S +W L P +QE + +I++ + L + +P A + E+ R +S HST D ++ YE+PKD++++ N++ +HHD ++W NPD DP FL + G+ ++P+ G R C+ + + LF+ I ++ ++
Sbjct: 19 YGEVFRLKVGSHRIVVVNSIEAAREALVRR-ANDFAGRPMLYTASLISRNGTDIAFGDFGPLWKQQRKIAHSALRMFGSGLKPLEEKITVEADELIKRLSLVEGRAHNP---KDDVNLAVLNIICSFVFGSRY-DMDDPEFHTVVDYTEQFVQ-----GFRAASLVDYFPWLRHLPNKGLRQIKEACQNRDVVLQKKYQEHLVTYEHGV-IRDLTDALLSANKAS----QEESAYLPMTEDHIIMTMNDIFSAGLETTATSILWSLVYLIRNPKVQEQLHQQIDEVVGRDRAP-ELSDRENLPLIEATIAEVLRSSSLVPLLFPHSTTTDTHLRGYEVPKDTIVLFNVWNMHHDPQRWSNPDEFDPTRFLDDKGKFICPATLSYLPFSAGRRVCLAESLGKMQLFLFISRLLHKFR 418
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Nematostella
Match: EDO46605 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPN7]) HSP 1 Score: 129.028 bits (323), Expect = 2.622e-32 Identity = 99/403 (24.57%), Postives = 190/403 (47.15%), Query Frame = 3
Query: 237 GTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIF--NDVDYKKYQGAIEVIPQYAEECDFPNDKPSKKS-DFKSVEKLQKVFNQLIEVLQIRIDEHR---KKGDYKIACFDITDFVLLAVEEY-HLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKM 1424
G V I++I+ +V ++K + V+ R+++Y +S N +++ Y P WK +R +H LR + R+ E + + + S+ ++ L NVI L+F + + +D ++ E + + A + P + + V+K+++ ++Q +I EHR K+G+ + D+TD +L ++E+ D + L LT+ H+ ++ DV A E + W+F +A YP +Q I E++ + KS +PY A + E+ R + +NIG Y IP+++ + +NL+AIHHD+ +W +P P FL +G + +P+G G+R CVG + ++ LF+ + ++ +K+
Sbjct: 70 GKRFVIIDNIKLARQVLLKKNGRDVADRTSAYVIDAISRNGCSVIFGKYSPTWKLQRRVVHSALRMYGSGVRILEAL--ILQEVDNLCDIFANSQGAPVDTKLPLTLSVMNVICALVFGKRYGLDDAEFTDIVATTESMAKLASPENPLIYMPWLRFFPLQVVKKMKETVTARDRLIQRKITEHRETYKEGEIR----DLTDALLKSLEDAKQEDNNAMSL---LTEDHLLIAINDVFMAGFETTTTALRWIFLYVAAYPEVQARIHQELDDVISFGRDPLIADRKS-LPYLEATILEVLRISPVFPLIR-RKARKQLNIGGYCIPEETFVYVNLWAIHHDEAEWVDPYKFRPSRFL--EGTNSASAYSMLPFGAGVRVCVGESLAKMELFLFVSRLLHRFKL 459
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Nematostella
Match: EDO49214 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHE7]) HSP 1 Score: 125.561 bits (314), Expect = 6.162e-31 Identity = 103/409 (25.18%), Postives = 183/409 (44.74%), Query Frame = 3
Query: 204 KYGPICTLYKDGTPVVFINDIETGYEVFVRKLSK--QVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAE---ECDFPNDKPSKKS-DFKSVEKLQKVFNQLIEVLQIRIDEHR---KKGDYKIACFDITDFVLLAVEEYHLDPKSKK---LGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVL 1394
KYGP+ L VV IND+ +++ S+ +GR+ S + SG+ +I Y W+ + L +RHC + + D+ ++ + + + DL + NV + ++F IF D ++Q ++ ++E +F + P + F ++K+++ ++ E+ + H+ K+G + DI D ++ A ++ + + S+ L L L ++L + I A E +W LLA YP++Q ++ I+ ++V +T +SK PY AV+ E R A+ H TM D + YEIPKD++ NL+ +HHD W+NP P + G +P+ LG R+C S +LFVL
Sbjct: 78 KYGPVYKLRLGRQLVVVINDL-----ALIKEASRGSDFAGRAKSISGEFFSGHGKSIGFQTYSEQWRGQHKALTIAIRHCEG--KQGPLEATVADEVEKLLGRLAKQADSTFDPRLDLGLLVTNVFSRVIFGRIFKKED-GEFQSLLQQTLNFSEGLAAANFLDAFPFLQVFPFPVIKKMRETASRRDEIFLSQHQLHQVTFKEGRIR----DIADALIQAEKDENQNIGSRPDADLPRSLDGLKRMYTLNESIIAGIESPLTCIMWTVALLAKYPDVQAKLQKSIDDIIGRSHVLLTYQDRSKFPYVEAVIYEALRLATVLPVAIPHFTMADAKLAGYEIPKDTIAFFNLWNVHHDPGLWENPYEFKPDRLIDSKGEVIT--AGLLPFSLGTRSCPWEVSSRKMLFVL 472
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Nematostella
Match: EDO41250 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S508]) HSP 1 Score: 125.176 bits (313), Expect = 6.706e-31 Identity = 111/469 (23.67%), Postives = 200/469 (42.64%), Query Frame = 3
Query: 186 VQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGP--LWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNG--------GIEVDNDLFWMADNVITMLLFN--------EIFNDVDYKKYQGAIEVIPQYAEECDFPNDKPSKKSDF--KSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACF-DITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHW-----KSKMPYTNAVLCEIQRYASADTFTDFH-STMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGR-SFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEPFNIQ 1508
K YG I L VF++ EV + K ++GR ++ +LS +I+ DY +W+ ++ +H GLR MY F K +E + + G I + N+I+ L + +++ + + V ++ F P +S F VE ++ ++ EH K + C D+TD L A E + K K +G ++TD H+ + DV E + W+ L YP +Q ++T+I+ F + W K +PY A + E+ R A+ H +T +++ + Y IPKD++IV+NL+AIHHD ++W NPD DP F++EDG+ ++ ++P+ G R CVG + ++ LF+++ ++ + + + + + + R +PF +
Sbjct: 53 AAKLAKTYGDIFMLKIFNDRFVFVSSGAGAREVLMGKRMNDLAGRPQTFTFNLLSRGAKDIVLGDYSRHLVWR--RKIVHLGLR-----------MY--FPKLEENIAWEVSKLEGKLMEDIGAAIHSKEVFLLLITNIISSFLLGKSYEPDDPDFLTTLEFIRIGSQVPVSGSILDKFPFLYHLPISESRFYKNVVEPRDRLLDKFFL-------EH--KSTFSEDCIRDLTDAFLKAKLEVEREDK-KAIG-EVTDDHITMLMTDVYVGGLETTSTTLSWLAAYLINYPLVQTRLQTDIDDF-------IGSRWPVLADKPNLPYMEATIAEVLRIATVLPMGVPHKATTNNVPLMGYNIPKDTIIVMNLWAIHHDPREWPNPDKFDPDRFINEDGKFEVPGQRSYLPFSAGRRVCVGESLAKIELFLVMTRLLQQFTLETLPGQGPPSVGEASGNITRAPKPFKFR 488
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Medaka
Match: cyp17 (cytochrome P-450 17alpha-hydroxylase/C17,20-lyase [Source:NCBI gene;Acc:100125816]) HSP 1 Score: 132.109 bits (331), Expect = 1.003e-32 Identity = 105/458 (22.93%), Postives = 208/458 (45.41%), Query Frame = 3
Query: 168 LIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGL----RHCANLDRLNEIMYKCFDKFKETVEYRRGSKNG-GIEVDNDLFWMADNVITMLLFNEIFNDVD------YKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIAC-FDITDFVLLA---VEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFK-RRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVE 1493
+++K++ QKY G +L V+ +N EV +++ + +GR + +L+ + +I DY W+ ++ +H L A+L R+ C + + G +++ +L NVI L FN ++ D + QG ++ + + + FP + D + +++ V +QL LQ + +EH K DY D+ D +L A E + + + L+D H+ ++ D+ A E W L YP +Q+ I+ E+++ + + L + +P+ A + E+ R H + D ++G + + K + +V+NL+++HHD+K+W NPD+ +P FLS DG S ++P+G GLR C+G ++ LF+ + ++ + + ++S ++ K V ++ V+
Sbjct: 56 VLFKELQQKY----GQTYSLKMGSHQVIIVNHHAHAREVLLKR-GRTFAGRPRTVTTDVLTRDGKDIAFGDYSATWRFHRKIVHGALCMFGEGSASLQRI-----ICTEAQSLCSTLSEAAATGLALDLSPELTRAVTNVICSLCFNSSYSRGDPEFEAMLRYSQGIVDTVAKDSLVDIFPWLQIFPNKDLRLLKQCVAVRDQL---LQKKFEEH--KSDYSDHVQRDLLDALLRAKRSAENNNTAAEFSAEAVGLSDDHLLMTVGDIFGAGVETTTTVLKWAITYLIHYPEVQKQIQEELDR-KVGVDRPPQLSDRGSLPFLEATIREVLRIRPVAPLLIPHVALSDTSLGDFTVRKGTRVVINLWSLHHDEKEWTNPDLFNPGRFLSADGSSLTLPSSSYLPFGAGLRVCLGEALAKMELFLFLSWILQRFTLSVPPSQSLPSLEGKFGVVLQPVK 497
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Medaka
Match: cyp17 (cytochrome P-450 17alpha-hydroxylase/C17,20-lyase [Source:NCBI gene;Acc:100125816]) HSP 1 Score: 132.109 bits (331), Expect = 1.950e-32 Identity = 105/458 (22.93%), Postives = 208/458 (45.41%), Query Frame = 3
Query: 168 LIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGL----RHCANLDRLNEIMYKCFDKFKETVEYRRGSKNG-GIEVDNDLFWMADNVITMLLFNEIFNDVD------YKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIAC-FDITDFVLLA---VEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFK-RRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVE 1493
+++K++ QKY Y +L V+ +N EV +++ + +GR + +L+ + +I DY W+ ++ +H L A+L R+ C + + G +++ +L NVI L FN ++ D + QG ++ + + + FP + D + +++ V +QL LQ + +EH K DY D+ D +L A E + + + L+D H+ ++ D+ A E W L YP +Q+ I+ E+++ + + L + +P+ A + E+ R H + D ++G + + K + +V+NL+++HHD+K+W NPD+ +P FLS DG S ++P+G GLR C+G ++ LF+ + ++ + + ++S ++ K V ++ V+
Sbjct: 134 VLFKELQQKYGQTY----SLKMGSHQVIIVNHHAHAREVLLKR-GRTFAGRPRTVTTDVLTRDGKDIAFGDYSATWRFHRKIVHGALCMFGEGSASLQRI-----ICTEAQSLCSTLSEAAATGLALDLSPELTRAVTNVICSLCFNSSYSRGDPEFEAMLRYSQGIVDTVAKDSLVDIFPWLQIFPNKDLRLLKQCVAVRDQL---LQKKFEEH--KSDYSDHVQRDLLDALLRAKRSAENNNTAAEFSAEAVGLSDDHLLMTVGDIFGAGVETTTTVLKWAITYLIHYPEVQKQIQEELDR-KVGVDRPPQLSDRGSLPFLEATIREVLRIRPVAPLLIPHVALSDTSLGDFTVRKGTRVVINLWSLHHDEKEWTNPDLFNPGRFLSADGSSLTLPSSSYLPFGAGLRVCLGEALAKMELFLFLSWILQRFTLSVPPSQSLPSLEGKFGVVLQPVK 575
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Medaka
Match: cyp21a2 (steroid 21-hydroxylase [Source:NCBI gene;Acc:101157155]) HSP 1 Score: 124.79 bits (312), Expect = 1.440e-30 Identity = 104/418 (24.88%), Postives = 186/418 (44.50%), Query Frame = 3
Query: 204 KYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEE--CDFPNDKPSKKSDFKSVEKL-QKVFNQLI-------EVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMV 1427
+YG I L T +V +N E E VRK S +GR SY I+SG NI DY W+ +R +H L+ C L+ ++ + ++ + +GS +++ D A NVIT L F + +Y K ++ + E + + S F + KL VF +L+ E+++ +D + +G IT +L A++++ ++ K G LTD+HV + +D++ E W L P IQ+ + E+ + + + + ++P A++ E+ R H + + +I + IPK+S+I+ NL+ HHD W P P+ FL G S + VP+G G R C+G + ++ LF+ ++ +++ +
Sbjct: 19 RYGSIYRLKCGNTTMVVLNSGELIRETLVRKWS-DFAGRPISYTGDIVSGGGRNISLGDYTEEWRAHRRLVHGALQRCCQ-QSLHSVIERQALHLRKVLMGYQGSP---VDLSEDFTVAASNVITTLAFGK-----EYDKSSSELQQLHSCLNEIVSLWGSSWISALDSFPLLRKLPNPVFARLLKEVARRDEIIKQHLDNFKLQGHQSED--SITGSLLQALDKH----QNTKEGQLLTDVHVHMATVDLLIGGTETTAAWLNWTVAFLLHRPEIQDKVYEELCTVPEGRYPKYSD--RQRLPVLCALVNEVLRLRPVAPLAVPHRAIRNSSIAGFFIPKNSVIIPNLFGAHHDPAVWSEPYSFKPERFLEGGGGS---TRALVPFGGGARLCLGESVAKMELFLFTAYLLRDFQFI 415
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Medaka
Match: ENSORLT00000024770.2 (cytochrome P450 2J6 [Source:NCBI gene;Acc:101169416]) HSP 1 Score: 125.176 bits (313), Expect = 1.980e-30 Identity = 108/442 (24.43%), Postives = 206/442 (46.61%), Query Frame = 3
Query: 207 YGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEY--RRGSKNGGIEVDNDLFW--MADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECDFPNDKPSKKSD-FKSVEKLQKVFNQ-------LIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEP 1496
YG + + + P V +N ++ E + K + SGR L+ R ++ DYG +W+ +R LR+ L +I + ++ E +++ + +N G + + A N+I +LFN ++ D K E++ + E N + D F + FN+ + + + ++EH+ ++A + DFV ++E K+KK ++ + D+ A + N+ + F L +YP+IQE + EI++ + + V+ + KMPY AV+ E+QR+A+ + FH T D + Y IPK +LI+ N+ ++ ++ QWK P +P+NFL++ G F + + F+P+ G R C+G + LF++++ ++ +K W ++ Q D + VF + P
Sbjct: 59 YGNVYSFFIGSRPAVVVNGLQAMKEAIMIK-ATDFSGRPQDMFVSDLT-QRKGVILVDYGSVWRDHRRFALMTLRNFG----LGKISME--ERIHEEIQFTISKLEENIGKTLSPKFMFHNAASNIICKVLFNTRYDYEDGK----ISEIVQGFVEMLQIANGPWAMMYDAFPWIRSFPLPFNKAFRNMENFLNLAKRLVNEHKMS---RVAG-EPRDFVDSYLDELE---KNKK---DFSEQQLLRMTFDLYIAGTDTTSNTMLAGFLYLMVYPHIQEKCQREIDQMLERKD-RVSFEDRHKMPYMQAVIHEVQRFANIVPLSVFHCTTKDSELSGYTIPKGTLIIQNMVSVLREEGQWKFPHEFNPENFLNDQG-EFIKPEAFMPFSAGPRMCLGEGLARMELFIIMVTLLRKFKFTWPEDAGQ---PDLSPVFGATLTP 473
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Medaka
Match: cyp17a2 (steroid 17-alpha-hydroxylase/17,20 lyase-like [Source:NCBI gene;Acc:101170547]) HSP 1 Score: 125.561 bits (314), Expect = 1.985e-30 Identity = 99/425 (23.29%), Postives = 185/425 (43.53%), Query Frame = 3
Query: 156 INEKLIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLR-HCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQ------GAIEVIPQ------YAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKL-GIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNV----EVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSE 1376
+ L++ K+ +Y G + L+ V +N + EV +++ + +GR + ++L+ +I SDY PLWK +R +H RL ++++ D + GS+ G + + NV+ L+F+ + D + + G +E I + Y FPN S+ KL++ +L +++EH+ +A D +D + ++ PKS L G +TD HV + + A E + +W+ L +P +Q+ ++ E+ D NV L + +PY + V+ E R H+ M D +IG + + + + +++N+++IHHD + W PD+ P+ FL + G F+P+G G R CVG + +
Sbjct: 69 LPPHLLFTKLAHRY----GSLFGLFLGPHYTVVVNSHQHAKEVLLQR-GRDFAGRPSMVTTELLTRGGKDIAFSDYSPLWKLHRRLVHNSFTLFGEGTSRLQDVVFSAVDSLCSEL-MSNGSR--GFDPSAAVTRAVTNVVCTLVFSSTYRHGDAELQEVISYNNGIVETIARGGLVDIYPWMKVFPNS---------SLRKLKECIAVRDRLLTRKLEEHKAS----LAEGDPSDLLDALLKGKKGGPKSHGLEGEGITDDHVLMTAAEAFGAGVETTSTTLLWILAYLLHHPEVQQRVQKEL-----DENVGADRAANLSDRGHLPYLDCVINEGMRIRPVSPVLIPHTAMTDSSIGGHAVRRGTRVLVNMWSIHHDPQNWDKPDLFQPERFLDDQGNR-ATPSCFMPFGAGPRVCVGESLAR 466
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Planmine SMEST
Match: SMESG000080703.1 (SMESG000080703.1) HSP 1 Score: 986.867 bits (2550), Expect = 0.000e+0 Identity = 479/480 (99.79%), Postives = 480/480 (100.00%), Query Frame = 3
Query: 84 MNRTISSYKLPPGPNRNSDEADFIINEKLIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEPFNIQFIRLK 1523
MNRTISSYKLPPGPNRNSDEADFIINEKLIY+KIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEPFNIQFIRLK
Sbjct: 1 MNRTISSYKLPPGPNRNSDEADFIINEKLIYQKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECDFPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQEIIDDKNAVFIRDVEPFNIQFIRLK 480
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Planmine SMEST
Match: SMESG000012534.1 (SMESG000012534.1) HSP 1 Score: 246.899 bits (629), Expect = 1.102e-75 Identity = 144/445 (32.36%), Postives = 240/445 (53.93%), Query Frame = 3
Query: 168 LIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFN--DVDYKKYQGAIEVIPQYAEECDFPNDKP-SKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKF-RDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVW-GKNKSQEIIDD-KNAVFIR 1484
+IYK++V+ Y PKYG + TL+ TP+VFIND + E K K ++GR + E I+S R+NI ++YGPLWK + R L G+ C L + + K + + + + + G+ ++ +W++DN + ++ F D DY Q + + ++ +F N P + K + +L + +L +L + + RK G Y+ +I +++L E L + K + L D+H++ D++FAS EG+G + +W LL L+P IQE IR +I K + WK+++PY AV E R++S DFH + + +G Y IPK LI +N YAI+HD ++W+NP+ I P+ FL G +F R D +P+G+G+R C G + ++ F+L+ + NYK+ W +N+ E++DD +NAV R
Sbjct: 1 MIYKRMVEYYKPKYGNLVTLFWGNTPIVFINDYKLAIEALGNKNGKHLAGRKSVQTEDIVSEERHNIFGNNYGPLWKEMIRALKLGVSKCGLTPSLEKNIQKSINFYCDELIRNPDNSGKGVFPFDESYWLSDNTVLSIVIGRTFTLEDEDYIILQDSRRQVFDIMDKSEFVNWIPFGRYLPLKCIRRLFEQSTKLNNLLAKFVSDARKMGRYRNNPDNIIEYLLQETENSKLVTEKK---VNLNDVHIRQIFKDILFASYEGIGMTNIWYMALLGLHPQIQERIRLDITKLCSKKKDSTPKFEWKNELPYIQAVEYEFFRFSSIVGILDFHCALKSVTLGGYHIPKGCLIAVNQYAINHDAERWENPETIKPERFLDSSG-AFTDRSDLMPFGMGVRMCPGTSITKGYGFLLLCNLCLNYKIKWSSENEFTELVDDPENAVLFR 441
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Planmine SMEST
Match: SMESG000012534.1 (SMESG000012534.1) HSP 1 Score: 239.195 bits (609), Expect = 8.876e-73 Identity = 141/440 (32.05%), Postives = 235/440 (53.41%), Query Frame = 3
Query: 183 IVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETVEYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFN--DVDYKKYQGAIEVIPQYAEECDFPNDKP-SKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKF-RDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVW-GKNKSQEIIDD-KNAVFIR 1484
+V+ Y PKYG + TL+ TP+VFIND + E K K ++GR + E I+S R+NI ++YGPLWK + R L G+ C L + + K + + + + + G+ ++ +W++DN + ++ F D DY Q + + ++ +F N P + K + +L + +L +L + + RK G Y+ +I +++L E L + K + L D+H++ D++FAS EG+G + +W LL L+P IQE IR +I K + WK+++PY AV E R++S DFH + + +G Y IPK LI +N YAI+HD ++W+NP+ I P+ FL G +F R D +P+G+G+R C G + ++ F+L+ + NYK+ W +N+ E++DD +NAV R
Sbjct: 1 MVEYYKPKYGNLVTLFWGNTPIVFINDYKLAIEALGNKNGKHLAGRKSVQTEDIVSEERHNIFGNNYGPLWKEMIRALKLGVSKCGLTPSLEKNIQKSINFYCDELIRNPDNSGKGVFPFDESYWLSDNTVLSIVIGRTFTLEDEDYIILQDSRRQVFDIMDKSEFVNWIPFGRYLPLKCIRRLFEQSTKLNNLLAKFVSDARKMGRYRNNPDNIIEYLLQETENSKLVTEKK---VNLNDVHIRQIFKDILFASYEGIGMTNIWYMALLGLHPQIQERIRLDITKLCSKKKDSTPKFEWKNELPYIQAVEYEFFRFSSIVGILDFHCALKSVTLGGYHIPKGCLIAVNQYAINHDAERWENPETIKPERFLDSSG-AFTDRSDLMPFGMGVRMCPGTSITKGYGFLLLCNLCLNYKIKWSSENEFTELVDDPENAVLFR 436
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Planmine SMEST
Match: SMESG000050434.1 (SMESG000050434.1) HSP 1 Score: 226.098 bits (575), Expect = 4.522e-67 Identity = 145/495 (29.29%), Postives = 247/495 (49.90%), Query Frame = 3
Query: 105 YKLPPGPNRNSDEADFII-------NEKLIYKKIVQKYIPKYGPICTLYKDGTPVVFINDIETGYEVFVRKLSKQVSGRSASYGEKILSGNRNNILNSDYGPLWKTLQRNLHKGLRHCANLDRLNEIMYKCFDKFKETV-EYRRGSKNGGIEVDNDLFWMADNVITMLLFNEIFNDVDYKKYQGAIEVIPQYAEECD---FPNDKPSKKSDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKI-------ACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWK--SKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRSFKRRKDFVPYGLGLRTCVGNTFS-ENVLFVLIMLVVDNYKMVW-GKNKSQEIIDDKNAVFIRDVEPFNIQFIRLK 1523
+ LPPGP +A II + L YK++++ + KYG I + G V+++D + GY+ FV+ S +++GR + +++ GN N W L++ + LR A ++ EI ++ F + E + +GG V+ +W+ N++ + + + + D YQ I VI + + F + P K+ + L + +Q I E + G+YK+ C +I D E+Y P LTD + + +D+ FA + G F W GLLA +P++Q+NIR E+ K D NVE + ++K K+ Y NAV+ E+ R S +FT FH M D+ +G Y+IPKD++I LN+Y H+D K WKNP+ +P F++ DG+ ++ F+P+G+G RTCV ++ + F+ + ++K+ W KNK+ ++ +D F R PF ++F +K
Sbjct: 31 HNLPPGP-----KAWPIIGSIPDPYSSPLSYKRLIEDWKEKYGEIILFKRMGMNFVYVSDYKLGYQAFVKDYSNELAGRPLNSVDRLSVGNENIFFTRSLDAGWTELRKQIMIMLRKVAEPQKMTEISNNAYELFATHIREKKELVSDGGFYVEQLCYWVGHNILVQVFWEDAV-EFDNPNYQDTISVIGKLMAKVGNLAFFDITPLNCLPHPGKNKILEYSKYLSDKVQKHIKERKSDGNYKVNPKYFIDYCIEIQDNQKEQKEKYGQLPM-------LTDWFISNLSLDIYFAGMDAFGIGFSWTIGLLATHPHVQDNIRKELQK-NDIENVE-SFNYKMMDKLHYLNAVVNEVMRIHSLSSFTSFHRAMSDVKVGEYDIPKDTIIALNIYGAHYDPKYWKNPNEFNPDRFINADGKFQSPKEGFMPFGVGRRTCVAERYTVKESHFIFTGRICQDFKITWSSKNKTDKVEEDLENTFFRHTAPFYVEFHSIK 510
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Planmine SMEST
Match: SMESG000078094.1 (SMESG000078094.1) HSP 1 Score: 117.472 bits (293), Expect = 6.969e-28 Identity = 76/275 (27.64%), Postives = 137/275 (49.82%), Query Frame = 3
Query: 699 SDFKSVEKLQKVFNQLIEVLQIRIDEHRKKGDYKIACFDITDFVLLAVEEYHLDPKSKKLGIKLTDLHVKHSLIDVIFASQEGLGNSFVWVFGLLALYPNIQENIRTEINKFRDDNNVEVTLHWKSKMPYTNAVLCEIQRYASADTFTDFHSTMDDINIGPYEIPKDSLIVLNLYAIHHDKKQWKNPDIIDPQNFLSEDGRS---FKRRKDFVPYGLGLRTCVGNTFSENVLFVLIMLVVDNYKMVWGKNKSQE-----IIDDKNAVFIRDVEPF 1499
SD+K K+Q F + I EHR D+ DI D L ++++ L + + D + + +++ A E + W ++ YP+IQE + EI++ + T++ K M YT +V+ E+ R +S + H T D NI + IPKDSLI NLYA H D + W++P P++FLS D ++ R++ +P+ +G R C+G + + + F+ + +V +++ +G+ S+E II ++ + D++ F
Sbjct: 238 SDWKRFVKIQDYFAE-------EIKEHRNALDFTSEGEDIMDRFL--IKQHELKQSTGNVE-SFVDWQLIRNSVELFIAGFETTSTTLNWCCLFMSKYPDIQEKVYKEISEIIGKERLP-TMNDKKAMNYTQSVMDEVLRLSSVSPLSLIHRTFHDANINGFYIPKDSLIFPNLYACHMDPEIWESPTTFYPEHFLSLDSNGISKYQPREELIPFSVGKRQCLGESLARSEYFIFFVTMVQRFQISFGEEISEEKYLQTIIGNEGLIRTSDIKNF 501 The following BLAST results are available for this feature:
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Steroid 17-alpha-hydroxylase/17,20 lyase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30033976 ID=SMED30033976|Name=Steroid 17-alpha-hydroxylase/17,20 lyase|organism=Schmidtea mediterranea sexual|type=transcript|length=1629bpback to top protein sequence of SMED30033976-orf-1 >SMED30033976-orf-1 ID=SMED30033976-orf-1|Name=SMED30033976-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=481bp MNRTISSYKLPPGPNRNSDEADFIINEKLIYKKIVQKYIPKYGPICTLYKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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