Partner of Y14 and mago

Overview
NamePartner of Y14 and mago
Smed IDSMED30033947
Length (bp)2706
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Partner of Y14 and mago (SMED30033947) t-SNE clustered cells

Violin plots show distribution of expression levels for Partner of Y14 and mago (SMED30033947) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Partner of Y14 and mago (SMED30033947) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Partner of Y14 and mago (SMED30033947) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033947

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30033947SMESG000050479.1 SMESG000026600.1 SMESG000019968.1 SMESG000018885.1 SMESG000013089.1 SMESG000008152.1 Contig36611newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30033947SMESG000050479.1 SMESG000026600.1 SMESG000019968.1 SMESG000018885.1 SMESG000013089.1 SMESG000008152.1 Contig36611uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Partner of Y14 and mago vs. Ensembl Human
Match: PYM1 (PYM homolog 1, exon junction complex associated factor [Source:HGNC Symbol;Acc:HGNC:30258])

HSP 1 Score: 64.3142 bits (155), Expect = 5.877e-11
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = 2 
Query: 2147 VRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRV 2278
            V  E G  Y+ +TQRPDGTWRK+++VKEGY+PQEEVP+Y +K V
Sbjct:    6 VTDETGGKYIASTQRPDGTWRKQRRVKEGYVPQEEVPVYENKYV 49
[Close]
BLAST of Partner of Y14 and mago vs. Ensembl Human
Match: PYM1 (PYM homolog 1, exon junction complex associated factor [Source:HGNC Symbol;Acc:HGNC:30258])

HSP 1 Score: 61.6178 bits (148), Expect = 5.847e-10
Identity = 25/36 (69.44%), Postives = 33/36 (91.67%), Query Frame = 2 
Query: 2171 YLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRV 2278
            Y+ +TQRPDGTWRK+++VKEGY+PQEEVP+Y +K V
Sbjct:   15 YIASTQRPDGTWRKQRRVKEGYVPQEEVPVYENKYV 50
[Close]
BLAST of Partner of Y14 and mago vs. Ensembl Celegans
Match: T20G5.9 (pep chromosome:WBcel235:III:10209840:10211093:1 gene:WBGene00011871.1 transcript:T20G5.9.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T20G5.9)

HSP 1 Score: 54.6842 bits (130), Expect = 2.423e-8
Identity = 25/45 (55.56%), Postives = 33/45 (73.33%), Query Frame = 2 
Query: 2147 VRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRVL 2281
            V  E+G  ++ ATQR DGTWRK ++VK GYIPQ+E P Y++K  L
Sbjct:   22 VETENGETFITATQRADGTWRKARRVKGGYIPQDEQPKYQNKMQL 66
[Close]
BLAST of Partner of Y14 and mago vs. Ensembl Fly
Match: Pym (gene:FBgn0034918 transcript:FBtr0072233)

HSP 1 Score: 64.3142 bits (155), Expect = 2.857e-11
Identity = 63/198 (31.82%), Postives = 101/198 (51.01%), Query Frame = 2 
Query: 2171 YLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKR---VLNKEENLRP-YIDLTSVLNEKNNHKQ----------------VGPAGGVIILEDQYLXXXXXXXXXA----------NIEALIKSTEKIFIHEKPSAPKSEIDPEXXXXXXXXXXXXXYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLE 2674
            ++PAT+RPDGTWRK ++VK+GY+PQEEVP+Y SK    V  ++  + P    L +  ++K   KQ                  PA GV+++        K  + +           +I ++ K+ E      K  A +  +DP     K+L+ L+KK R+I +++ RI+A     LD DQ  K+ K++EI  +I+ LE
Sbjct:   12 FIPATKRPDGTWRKARRVKDGYVPQEEVPLYESKGKQFVAQRQAGVPPGMCPLLAAESKKEREKQERTRAKKQEKESGRQPKAPAPGVLVMPPSTCPPPKVSQQQQQQQQQPSGSRDINSISKTLEDTL---KLDAAQEVVDP----AKQLKKLRKKIREIEQIESRIQAGEQKKLDKDQLDKVKKKSEILRQIKDLE 202
[Close]
BLAST of Partner of Y14 and mago vs. Ensembl Zebrafish
Match: pym1 (PYM homolog 1, exon junction complex associated factor [Source:NCBI gene;Acc:393566])

HSP 1 Score: 57.7658 bits (138), Expect = 6.457e-9
Identity = 23/36 (63.89%), Postives = 32/36 (88.89%), Query Frame = 2 
Query: 2171 YLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRV 2278
            Y+ ATQRPDG+WRK ++V++GY+PQEEVP+Y +K V
Sbjct:   13 YIAATQRPDGSWRKPRRVRDGYVPQEEVPVYENKFV 48
[Close]
BLAST of Partner of Y14 and mago vs. Ensembl Xenopus
Match: pym1 (PYM homolog 1, exon junction complex associated factor [Source:NCBI gene;Acc:549028])

HSP 1 Score: 63.1586 bits (152), Expect = 1.253e-10
Identity = 26/36 (72.22%), Postives = 33/36 (91.67%), Query Frame = 2 
Query: 2171 YLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRV 2278
            Y+ ATQRPDG+WRK++KVKEGY+PQEEVP+Y +K V
Sbjct:   13 YIAATQRPDGSWRKQRKVKEGYVPQEEVPVYENKYV 48
[Close]
BLAST of Partner of Y14 and mago vs. Ensembl Xenopus
Match: pym1 (PYM homolog 1, exon junction complex associated factor [Source:NCBI gene;Acc:549028])

HSP 1 Score: 63.1586 bits (152), Expect = 1.328e-10
Identity = 26/36 (72.22%), Postives = 33/36 (91.67%), Query Frame = 2 
Query: 2171 YLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRV 2278
            Y+ ATQRPDG+WRK++KVKEGY+PQEEVP+Y +K V
Sbjct:   12 YIAATQRPDGSWRKQRKVKEGYVPQEEVPVYENKYV 47
[Close]
BLAST of Partner of Y14 and mago vs. Ensembl Mouse
Match: Pym1 (PYM homolog 1, exon junction complex associated factor [Source:MGI Symbol;Acc:MGI:1925678])

HSP 1 Score: 64.6994 bits (156), Expect = 3.373e-11
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = 2 
Query: 2147 VRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRV 2278
            V  E G  Y+ +TQRPDGTWRK+++VKEGY+PQEEVP+Y +K V
Sbjct:    6 VTDETGGKYIASTQRPDGTWRKQRRVKEGYVPQEEVPVYENKYV 49
[Close]
BLAST of Partner of Y14 and mago vs. Ensembl Mouse
Match: Pym1 (PYM homolog 1, exon junction complex associated factor [Source:MGI Symbol;Acc:MGI:1925678])

HSP 1 Score: 61.6178 bits (148), Expect = 3.946e-10
Identity = 25/36 (69.44%), Postives = 33/36 (91.67%), Query Frame = 2 
Query: 2171 YLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRV 2278
            Y+ +TQRPDGTWRK+++VKEGY+PQEEVP+Y +K V
Sbjct:   15 YIASTQRPDGTWRKQRRVKEGYVPQEEVPVYENKYV 50
[Close]
BLAST of Partner of Y14 and mago vs. UniProt/SwissProt
Match: sp|Q2F5J3|PYM_BOMMO (Partner of Y14 and mago OS=Bombyx mori OX=7091 GN=Pym PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.381e-14
Identity = 65/179 (36.31%), Postives = 94/179 (52.51%), Query Frame = 2 
Query: 2159 DGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSK-RVLNKEEN------LRPYIDLTSVLNEKNNHKQVGPAGGVIILEDQYLXXXXXXXXXANIEALIKSTEKIFIHEKPSAPKSEIDPEXXXXXXXXXX----XXXYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEI 2662
            DG  ++PATQRPDGTWRK +++KEGY+PQEEVP+Y SK +     +N      L P I +     +K     + P  G+II  ++     K KK    +E   +   K  I E P+ P   +  E   +          +KK R+I  L+++I+A  +   D DQK K+SK+ EI NEI
Sbjct:   12 DGSKFIPATQRPDGTWRKPRRIKEGYVPQEEVPLYESKGKQFRARQNDGLPVGLTPEI-VAQAQKKKGQRSTIQPIPGMIITVEKK---KKKKKTVTGVEEAAEKLAKCEIQE-PTLPSQSVPTESISQSDPTKRLKNLRKKLREIEFLEEKIKAGLLKSPDKDQKEKMSKKNEILNEI 185
[Close]
BLAST of Partner of Y14 and mago vs. UniProt/SwissProt
Match: sp|B4NSP6|PYM_DROSI (Partner of Y14 and mago OS=Drosophila simulans OX=7240 GN=Pym PE=3 SV=2)

HSP 1 Score: 69.707 bits (169), Expect = 4.059e-12
Identity = 64/195 (32.82%), Postives = 102/195 (52.31%), Query Frame = 2 
Query: 2171 YLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKR---VLNKEENLRP-YIDLTSVLNEKNNHKQ----------------VGPAGGVIILEDQYLXXXXXXXXXA-------NIEALIKSTEKIFIHEKPSAPKSEIDPEXXXXXXXXXXXXXYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLE 2674
            ++PAT+RPDGTWRK ++VK+GY+PQEEVP+Y SK    V  ++  + P    L +  ++K   KQ                  PA GV+++        K  + +        +I ++ K+ E      K  AP+  +DP     K+L+ L+KK R+I +++ RI+A     LD DQ  K+ K++EI  +I+ LE
Sbjct:   12 FIPATKRPDGTWRKARRVKDGYVPQEEVPLYESKGKQFVAQRQAGVPPGMCPLVAAESKKEREKQERTRAKKQEKESGRQPKAPAPGVLVMPPSTCPPPKVSQQQQQQPSGSRDINSISKAMEDTL---KLDAPQEVVDP----AKQLKKLRKKIREIEQIESRIQAGEQKKLDKDQLDKVKKKSEILRQIKDLE 199
[Close]
BLAST of Partner of Y14 and mago vs. UniProt/SwissProt
Match: sp|A6QPH1|PYM1_BOVIN (Partner of Y14 and mago OS=Bos taurus OX=9913 GN=PYM1 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 7.140e-11
Identity = 58/187 (31.02%), Postives = 92/187 (49.20%), Query Frame = 2 
Query: 2147 VRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSK--RVLNKEENLRPYIDLTSVLNEKNNHKQVGPAGGVIILED------QYLXXXXXXXXXANIEALIKSTEKIFIHEK---PSAPK-SEIDPEXXXXXXXXXXXXX-----------YRQIIELKKRIEADPIYVLDDDQKLKLSK 2638
            V  E G  Y+ +TQRPDGTWRK+++VKEGY+PQEEVP+Y +K  +    +  L P +   +      +     P GG   L        +  +K + ++ K   EAL ++ EK+ + E    PSAP+ S   P  + ++       +            RQ+ EL++RI+A  I     +Q  KL++
Sbjct:    6 VTDETGGKYIASTQRPDGTWRKQRRVKEGYVPQEEVPVYENKYVKFFKSKPELPPGLSPEATAPITASR----PEGGEPALSKTAKRNLKRKEKRRQQQEKGEAEALSRTLEKVSLGETAQVPSAPQASRAAPTAASDQPDSAATTEKAKKIKNLKKKLRQVEELQQRIQAGEISQPSKEQLEKLAR 188
[Close]
BLAST of Partner of Y14 and mago vs. UniProt/SwissProt
Match: sp|P82804|PYM_DROME (Partner of Y14 and mago OS=Drosophila melanogaster OX=7227 GN=Pym PE=1 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.864e-10
Identity = 63/198 (31.82%), Postives = 101/198 (51.01%), Query Frame = 2 
Query: 2171 YLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKR---VLNKEENLRP-YIDLTSVLNEKNNHKQ----------------VGPAGGVIILEDQYLXXXXXXXXXA----------NIEALIKSTEKIFIHEKPSAPKSEIDPEXXXXXXXXXXXXXYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLE 2674
            ++PAT+RPDGTWRK ++VK+GY+PQEEVP+Y SK    V  ++  + P    L +  ++K   KQ                  PA GV+++        K  + +           +I ++ K+ E      K  A +  +DP     K+L+ L+KK R+I +++ RI+A     LD DQ  K+ K++EI  +I+ LE
Sbjct:   12 FIPATKRPDGTWRKARRVKDGYVPQEEVPLYESKGKQFVAQRQAGVPPGMCPLLAAESKKEREKQERTRAKKQEKESGRQPKAPAPGVLVMPPSTCPPPKVSQQQQQQQQQPSGSRDINSISKTLEDTL---KLDAAQEVVDP----AKQLKKLRKKIREIEQIESRIQAGEQKKLDKDQLDKVKKKSEILRQIKDLE 202
[Close]
BLAST of Partner of Y14 and mago vs. UniProt/SwissProt
Match: sp|Q640E9|PYM1_XENLA (Partner of Y14 and mago OS=Xenopus laevis OX=8355 GN=pym1 PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 4.548e-10
Identity = 26/42 (61.90%), Postives = 35/42 (83.33%), Query Frame = 2 
Query: 2153 GEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRV 2278
             +D   Y+ +TQRPDG+WRK++KVKEGY+PQEEVP+Y +K V
Sbjct:    7 SDDSGKYIASTQRPDGSWRKQRKVKEGYVPQEEVPVYENKYV 48
[Close]
BLAST of Partner of Y14 and mago vs. TrEMBL
Match: A0A2A4JR05 (Mago-bind domain-containing protein OS=Heliothis virescens OX=7102 GN=B5V51_14183 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.401e-17
Identity = 75/180 (41.67%), Postives = 109/180 (60.56%), Query Frame = 2 
Query: 2159 DGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSK-RVLNKEENLRPYIDLT----SVLNEKNNHKQVGPAGGVIILEDQYLXXXXX-XXXXANIEALIKSTEKIFIHEKPSAPK-SEIDPEXXXXXXXXXXXXXYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLEK 2677
            DGC ++PATQRPDGTWRK +++K+GY+PQEEVP+Y SK +     +N    + LT    +   +K N   + P  G+II  D+  KK K   +  A+  + I+ TE  F    PSAP  +  DP     K+L+NL+KK R+I  L+++I++  +   D DQK KLSK+ +I  EI+ LEK
Sbjct:   11 DGCKFIPATQRPDGTWRKPRRIKDGYVPQEEVPLYESKGKQFKARQNTGLPVGLTPEIVAQAQKKKNPGVIQPIPGMIITVDKKKKKKKTGTEEVADKLSKIEITEPNFTTPSPSAPSNTTTDP----VKRLKNLRKKLREIETLEEKIKSGNLKTPDKDQKEKLSKKMDIVKEIEVLEK 186
[Close]
BLAST of Partner of Y14 and mago vs. TrEMBL
Match: A0A2H1WK89 (SFRICE_007003 OS=Spodoptera frugiperda OX=7108 GN=SFRICE_007003 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 2.389e-16
Identity = 75/183 (40.98%), Postives = 107/183 (58.47%), Query Frame = 2 
Query: 2159 DGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSK-RVLNKEEN------LRPYIDLTSVLNEKNNHKQVGPAGGVIILEDQYLXXXXXXXXXANIEALIKS--TEKIFIHEKPSAP-KSEIDPEXXXXXXXXXXXXXYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLEK 2677
            DGC ++PATQRPDGTWRK +++K+GY+PQEEVP+Y SK +     +N      L P +   +   + NN   + P  G++I  D+  KK K    +A  E L K   TE  F    PS P  S  DP     K+L+NL+KK R+I  L+++I++  +   D DQK K+SK+ +I  EI+ LEK
Sbjct:   11 DGCKFIPATQRPDGTWRKPRRIKDGYVPQEEVPLYESKGKQFKARQNTGLPVGLPPEVAAQAQKKKNNNPGVIQPIPGMLINVDKKKKKKKSGADEA-AEKLAKCEITEPNFSTPSPSTPTNSATDP----VKRLKNLRKKLREIETLEEKIKSGLLKTPDKDQKEKMSKKNDIIKEIELLEK 188
[Close]
BLAST of Partner of Y14 and mago vs. TrEMBL
Match: A0A1S3K099 (partner of Y14 and mago OS=Lingula unguis OX=7574 GN=LOC106177672 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 2.894e-16
Identity = 68/190 (35.79%), Postives = 114/190 (60.00%), Query Frame = 2 
Query: 2117 MNMKYERQGVVRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSK--RVLNKEENLRPYIDLTSVLNEKNNHKQVGPAGGVIILEDQYLXXXXXXXXXANIEALIKSTEKIFIH--EKPSAPKSEIDPEXXXXXXXXXXXXXYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLE 2674
            M    ER GVVR E+G +++PA++RPDGTWRK ++VK+GY+PQEEVP+Y SK  + +  +  L P +      ++ NN    G  G     +    +K K K+   ++ +L  S ++  ++  EK + P+ ++DP     K+++ LKKK +QI +L+ RI++  +   D DQ  K+S++ +I  EI++LE
Sbjct:   19 MAGALERDGVVRDEEGNMFIPASRRPDGTWRKARRVKDGYVPQEEVPVYESKGTQFVKNKPTLPPGL-FVEPDSKHNNPGSAGIPGLSKSAKKNAKRKEKKKQEAGDVTSLSSSLQETKLNDKEKDAKPEVKVDP----AKRIKTLKKKIKQIEDLQARIDSGDLKEPDKDQLEKISRKQQILTEIEELE 203
[Close]
BLAST of Partner of Y14 and mago vs. TrEMBL
Match: G4V9D4 (Mago-bind domain-containing protein OS=Schistosoma mansoni OX=6183 GN=Smp_091360 PE=4 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 2.647e-13
Identity = 36/50 (72.00%), Postives = 41/50 (82.00%), Query Frame = 2 
Query: 2129 YERQGVVRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRV 2278
            + RQGVVR E+G L +P TQRPDGTWRK  +VKEGYIPQEEVP+YRS  V
Sbjct:    4 FVRQGVVRDENGNLIIPPTQRPDGTWRKAVRVKEGYIPQEEVPLYRSSGV 53
[Close]
BLAST of Partner of Y14 and mago vs. TrEMBL
Match: Q5DDY3 (SJCHGC09415 protein OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 4.505e-13
Identity = 35/50 (70.00%), Postives = 42/50 (84.00%), Query Frame = 2 
Query: 2129 YERQGVVRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRV 2278
            + RQGVVR ++G L +P+TQRPDGTWRK  +VKEGYIPQEEVP+YRS  V
Sbjct:    4 FVRQGVVRDDNGNLIIPSTQRPDGTWRKAVRVKEGYIPQEEVPLYRSAGV 53
[Close]
BLAST of Partner of Y14 and mago vs. Ensembl Cavefish
Match: pym1 (PYM homolog 1, exon junction complex associated factor [Source:NCBI gene;Acc:103026776])

HSP 1 Score: 62.003 bits (149), Expect = 2.004e-10
Identity = 26/36 (72.22%), Postives = 32/36 (88.89%), Query Frame = 2 
Query: 2171 YLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRV 2278
            Y+ ATQRPDGTWRK ++VKEGY+PQEEVP+Y +K V
Sbjct:   13 YIAATQRPDGTWRKPRRVKEGYVPQEEVPVYENKYV 48
[Close]
BLAST of Partner of Y14 and mago vs. Ensembl Cavefish
Match: pym1 (PYM homolog 1, exon junction complex associated factor [Source:NCBI gene;Acc:103026776])

HSP 1 Score: 61.6178 bits (148), Expect = 6.121e-10
Identity = 26/36 (72.22%), Postives = 32/36 (88.89%), Query Frame = 2 
Query: 2171 YLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRV 2278
            Y+ ATQRPDGTWRK ++VKEGY+PQEEVP+Y +K V
Sbjct:   16 YIAATQRPDGTWRKPRRVKEGYVPQEEVPVYENKYV 51
[Close]
BLAST of Partner of Y14 and mago vs. Ensembl Nematostella
Match: EDO31659 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SX96])

HSP 1 Score: 56.9954 bits (136), Expect = 1.416e-10
Identity = 25/38 (65.79%), Postives = 32/38 (84.21%), Query Frame = 2 
Query: 2159 DGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSK 2272
            +G  ++PATQRPDGTWRK ++VK+GYIPQ+EV  Y SK
Sbjct:   11 EGDSWIPATQRPDGTWRKARRVKDGYIPQDEVEKYESK 48
[Close]
BLAST of Partner of Y14 and mago vs. Ensembl Medaka
Match: pym1 (PYM homolog 1, exon junction complex associated factor [Source:NCBI gene;Acc:101173466])

HSP 1 Score: 66.2402 bits (160), Expect = 6.960e-12
Identity = 61/192 (31.77%), Postives = 105/192 (54.69%), Query Frame = 2 
Query: 2171 YLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSK--RVLNKEENLRPYIDLTSVLNEKNNHKQVGP---AGGV-------IILEDQYLXXXXXXXXXANIEALIKSTEKIFIHEK--PSA--------PKSEIDPEXXXX--XXXXXXXXXYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLE 2674
            Y+ ATQRPDGTWRK ++VKEGY+PQEEVP+Y +K  +    + +L P +  +    ++           A G+       +  +++  ++ + +  +A +EA+  + E + I E+  PS+        P     PE + E  KK++NLKKK RQ+ EL++++++  I     DQ  KL +   ++ E++QLE
Sbjct:   13 YIAATQRPDGTWRKPRRVKEGYVPQEEVPVYENKYVKFFKSKPDLPPGLSSSEAAQQQQKTTPASAESEAAGLSKSAKRNMKRKEKRKQQHQDQDGEAEVEAVSNAVENVSISERDEPSSKAEPAVPVPSLHDSPETAAERVKKIKNLKKKLRQVEELQQKVDSGEIKQPTKDQLEKLGRAEALREELEQLE 204
[Close]
BLAST of Partner of Y14 and mago vs. Planmine SMEST
Match: SMESG000071754.1 (SMESG000071754.1)

HSP 1 Score: 317.775 bits (813), Expect = 2.664e-102
Identity = 184/186 (98.92%), Postives = 184/186 (98.92%), Query Frame = 2 
Query: 2117 MNMKYERQGVVRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRVLNKEENLRPYIDLTSVLNEKNNHKQVGPAGGVIILEDQYLXXXXXXXXXANIEALIKSTEKIFIHEKPSAPKSEIDPEXXXXXXXXXXXXXYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLE 2674
            MNMKYERQGVVRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRVLNKEENLRPYIDLTSVLNEKNNHKQVGPAGGVIILEDQYLKK KPKKPKANIEALIKSTEKI IHEKPSAPKSEIDPELSKEKKLRNLKKKYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLE
Sbjct:    1 MNMKYERQGVVRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRVLNKEENLRPYIDLTSVLNEKNNHKQVGPAGGVIILEDQYLKKAKPKKPKANIEALIKSTEKICIHEKPSAPKSEIDPELSKEKKLRNLKKKYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLE 186
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BLAST of Partner of Y14 and mago vs. Planmine SMEST
Match: SMESG000071754.1 (SMESG000071754.1)

HSP 1 Score: 313.153 bits (801), Expect = 1.416e-100
Identity = 182/184 (98.91%), Postives = 182/184 (98.91%), Query Frame = 2 
Query: 2123 MKYERQGVVRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRVLNKEENLRPYIDLTSVLNEKNNHKQVGPAGGVIILEDQYLXXXXXXXXXANIEALIKSTEKIFIHEKPSAPKSEIDPEXXXXXXXXXXXXXYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLE 2674
            MKYERQGVVRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRVLNKEENLRPYIDLTSVLNEKNNHKQVGPAGGVIILEDQYLKK KPKKPKANIEALIKSTEKI IHEKPSAPKSEIDPELSKEKKLRNLKKKYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLE
Sbjct:    1 MKYERQGVVRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYRSKRVLNKEENLRPYIDLTSVLNEKNNHKQVGPAGGVIILEDQYLKKAKPKKPKANIEALIKSTEKICIHEKPSAPKSEIDPELSKEKKLRNLKKKYRQIIELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLE 184
The following BLAST results are available for this feature:
BLAST of Partner of Y14 and mago vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
PYM15.877e-1163.64PYM homolog 1, exon junction complex associated fa... [more]
PYM15.847e-1069.44PYM homolog 1, exon junction complex associated fa... [more]
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BLAST of Partner of Y14 and mago vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
T20G5.92.423e-855.56pep chromosome:WBcel235:III:10209840:10211093:1 ge... [more]
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BLAST of Partner of Y14 and mago vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Pym2.857e-1131.82gene:FBgn0034918 transcript:FBtr0072233[more]
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BLAST of Partner of Y14 and mago vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pym16.457e-963.89PYM homolog 1, exon junction complex associated fa... [more]
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BLAST of Partner of Y14 and mago vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pym11.253e-1072.22PYM homolog 1, exon junction complex associated fa... [more]
pym11.328e-1072.22PYM homolog 1, exon junction complex associated fa... [more]
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BLAST of Partner of Y14 and mago vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Pym13.373e-1163.64PYM homolog 1, exon junction complex associated fa... [more]
Pym13.946e-1069.44PYM homolog 1, exon junction complex associated fa... [more]
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BLAST of Partner of Y14 and mago vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q2F5J3|PYM_BOMMO1.381e-1436.31Partner of Y14 and mago OS=Bombyx mori OX=7091 GN=... [more]
sp|B4NSP6|PYM_DROSI4.059e-1232.82Partner of Y14 and mago OS=Drosophila simulans OX=... [more]
sp|A6QPH1|PYM1_BOVIN7.140e-1131.02Partner of Y14 and mago OS=Bos taurus OX=9913 GN=P... [more]
sp|P82804|PYM_DROME2.864e-1031.82Partner of Y14 and mago OS=Drosophila melanogaster... [more]
sp|Q640E9|PYM1_XENLA4.548e-1061.90Partner of Y14 and mago OS=Xenopus laevis OX=8355 ... [more]
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BLAST of Partner of Y14 and mago vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2A4JR051.401e-1741.67Mago-bind domain-containing protein OS=Heliothis v... [more]
A0A2H1WK892.389e-1640.98SFRICE_007003 OS=Spodoptera frugiperda OX=7108 GN=... [more]
A0A1S3K0992.894e-1635.79partner of Y14 and mago OS=Lingula unguis OX=7574 ... [more]
G4V9D42.647e-1372.00Mago-bind domain-containing protein OS=Schistosoma... [more]
Q5DDY34.505e-1370.00SJCHGC09415 protein OS=Schistosoma japonicum OX=61... [more]
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BLAST of Partner of Y14 and mago vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pym12.004e-1072.22PYM homolog 1, exon junction complex associated fa... [more]
pym16.121e-1072.22PYM homolog 1, exon junction complex associated fa... [more]
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BLAST of Partner of Y14 and mago vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Partner of Y14 and mago vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Partner of Y14 and mago vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO316591.416e-1065.79Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Partner of Y14 and mago vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pym16.960e-1231.77PYM homolog 1, exon junction complex associated fa... [more]
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BLAST of Partner of Y14 and mago vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000071754.12.664e-10298.92SMESG000071754.1[more]
SMESG000071754.11.416e-10098.91SMESG000071754.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033947 ID=SMED30033947|Name=Partner of Y14 and mago|organism=Schmidtea mediterranea sexual|type=transcript|length=2706bp
ATATGTGTATATATATATATATTGAAAATATTTTGAAATATTGTGGGGCT
ATAAGCCATAATTTAAACAAAAATGAGAAAATTGTGTAAATTAAAACAAA
ACAAATGAGGTTTGGAAATTATCAATATGGCTGCTGCAACATAAGCACGC
ACCGCAAGCAGAAATCAAAAATAAAATAAAACATTAAATAAAACAAAATA
AAAACATAACAAAATTAAATAAAACACATAATAATTAAAATCATTGAAAA
GCCTACAGATGCAGAAAGAAACACAAAACTAAAATATAAAACCTCCAAAA
CCTTGCAAAAGAAGGTTTAGAAAATACATAGGCTGAAGTGGTGACCAAAA
TTTTAAATATCAAAGTGTAAAAAAAGAAACAATCAAAGAGAATGGTTAAA
TAATAAAAAATAAAGATTTACCAAAAATTTAGAAAATAATATCAAAAAAT
AGAGAAAAATAAGGAGAAAAATAAAATATTTAAAATAAAATAAAAGCAAT
AAAACCCCCAGAAAACTAGCCGAAAAAGGCTCAAAATACTCTGATTGGTC
GCACCCAGGCATGAAAATACTATATATACAGCAGTCATTTTCATAATTTT
TAGACATTTGGTGAACATGTTGTAGACACAAAATTTTAAGACAGTTGTTG
AGAAAATTTTTATGCCAACGTATGACAATTTTTCAAATTTGGATGCTTGA
CTGACAAACTTGTCGATCGTAAGTATTGTTTTTTGTAGAAATATATTTTT
CTAAGTTAATGTTTTGTTTAATTTAACTTATTCTGGATGGCCGCGATGTC
TTTTGGCATCGCTCGGCAGGTTCCCTTTAACTTATTTATATTCATTTTAC
ATATATTAGGTCAGTGGATAAGTGGTGAAACGACATTCAATTCCGGCAGT
TAAATTTGCAGTATGTTGATGTGTCGTTTTATCATTGACCACCCTTCGGT
GTCTGCTTAGTTTGGTCAGGCCTTGTTTGCACTGATCACGTGGCAGGACA
CCGTCGGAATTCACCGTGGCGGCCATTGCGATGTTGGTCGGAGCGCTAAA
CTGCGGGAAGATCGCCATAATTAAGGGGATAACATTTGTGTAAATGTTTA
AGTAGGAAAATTGAATTTTTAATTTAACTCCTGCCGATTATTATATTATC
AGGGGTACGACTAGTGATAGAAATTTAGGTCAACGAAAGTATAAAGTATG
GTTAAATCACGTACATTGTTTGATAATCATCGCGGTGGAAAATTAAAATA
AAATGATACGTTTGGGCATAGATCTGTGAGATGATAATATAGAGATTTAC
GAATGGTGGTAACTGTGGCGACCATAGGAAAGCAAATATGAGTTGTTGGA
CTAAAATAATGACATTATAATAATGCATTCATTTCAATTTACCATAGGCT
AACGTTTGTATGAAACAAGGAATTTTCCATAAACTAAGGGCGGAACGCTG
CCCAAATTTTTGAAAAATATTTCAATAAATAAACAGTAATGATGACAAAT
TGATATAGGAATTGTAGAAATAGATGAGTATGGACAAGCGTATAGATAGA
AAGAGACGTAAGACCTTCCATCGGCAGCCAGGAACCCGAAAGTGAAAATA
TCATGAGTAGCTTTAGAAATGGAGCTCACCTGCTCATGAATTTTCAGCTG
CTATATACAGTACCAACATGAGGTTGCTCGTGGGTATGTAGTGGTAGGAT
TAAAATTGTGGGGCGTCTGGGTGAAGTAGCATAGACCCTAGGAAAAAGAC
GTATTTTGATCGAGGAGAATAAGTTTGAATAAGCTTTGCACAGATTTAAA
CAAATTAGTTACACCCAGTACACCAGCAATATGGACAAGTCATTGTAATA
GCCCTTGAATATATAGCGAACCTGACATCGAATTGAACATATAATGAACC
GTTCAACAAAGTGCTCCGCATTGGTTTCTAACACAATGGCCCCTTGTAGT
AGTGAATATTGTACCACCTGGCTCACTTTTTGGTACCTCCTATACTACTT
ATTAGATTATAAAGATTAATATTATGTATTTGATTTCTTAACTTATTTTT
GTTTTAATTTTTTATTATTATTTTAATAATTAATTTATTTTTAATTTAAT
TAATTTATTATTTAATATGAATATGAAATATGAACGTCAAGGTGTTGTTC
GCGGTGAAGATGGCTGCCTTTATTTACCCGCTACTCAAAGACCAGATGGT
ACTTGGAGGAAACGACAAAAAGTCAAAGAAGGATATATTCCTCAAGAGGA
AGTTCCTATATATCGCTCAAAAAGAGTTCTTAACAAAGAAGAAAATCTTC
GCCCTTATATAGATTTGACTAGTGTGTTAAATGAGAAAAATAACCACAAA
CAAGTTGGACCTGCTGGTGGAGTAATAATTCTTGAAGATCAATATTTAAA
GAAGACAAAACCAAAGAAACCAAAAGCCAATATCGAAGCACTTATTAAAT
CCACAGAAAAAATTTTTATTCATGAAAAACCATCAGCTCCAAAAAGTGAA
ATAGATCCAGAACTATCTAAAGAGAAAAAACTTCGAAATCTCAAAAAAAA
ATATCGGCAAATCATTGAATTAAAAAAACGTATCGAAGCCGATCCAATTT
ACGTACTTGACGATGACCAGAAACTGAAATTGTCAAAAGAAGCTGAAATT
AAAAATGAAATTCAACAATTGGAAAAAGAGTGATAAAATCTTTCTAAAAA
GATCGG
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protein sequence of SMED30033947-orf-1

>SMED30033947-orf-1 ID=SMED30033947-orf-1|Name=SMED30033947-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=189bp
MNMKYERQGVVRGEDGCLYLPATQRPDGTWRKRQKVKEGYIPQEEVPIYR
SKRVLNKEENLRPYIDLTSVLNEKNNHKQVGPAGGVIILEDQYLKKTKPK
KPKANIEALIKSTEKIFIHEKPSAPKSEIDPELSKEKKLRNLKKKYRQII
ELKKRIEADPIYVLDDDQKLKLSKEAEIKNEIQQLEKE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:1903259exon-exon junction complex disassembly
GO:0000184nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
Vocabulary: INTERPRO
TermDefinition
IPR015362WIBG_fam
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005737cytoplasm
GO:0035145exon-exon junction complex