SMED30033876

Overview
NameSMED30033876
Smed IDSMED30033876
Length (bp)350
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30033876 (SMED30033876) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30033876 (SMED30033876) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30033876 (SMED30033876) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30033876 (SMED30033876) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033876

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30033876 SmedASXL_001384SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30033876 dd_Smed_v4_3731_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30033876 dd_Smed_v4_3731_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30033876 dd_Smed_v4_3731_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30033876 dd_Smed_v4_3731_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30033876 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001512:13662..14011 -1750
Homology
[Close]
BLAST of SMED30033876 vs. Ensembl Human
Match: URM1 (ubiquitin related modifier 1 [Source:HGNC Symbol;Acc:HGNC:28378])

HSP 1 Score: 117.857 bits (294), Expect = 3.913e-35
Identity = 57/97 (58.76%), Postives = 74/97 (76.29%), Query Frame = -2 
Query:   41 MRLTLEFSGGAELLVGKIKKHEVNVPDNVKI--MKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 325
            + + +EF GGAELL   IKKH V +P   +   +++LL+WIK +L+KERPELF+QGDSVRPGILVLIND DW L  E+ Y L + D++LF+STLHGG
Sbjct:    5 LSVEVEFGGGAELLFDGIKKHRVTLPGQEEPWDIRNLLIWIKKNLLKERPELFIQGDSVRPGILVLINDADWELLGELDYQLQDQDSVLFISTLHGG 101
[Close]
BLAST of SMED30033876 vs. Ensembl Human
Match: URM1 (ubiquitin related modifier 1 [Source:HGNC Symbol;Acc:HGNC:28378])

HSP 1 Score: 90.8929 bits (224), Expect = 5.819e-24
Identity = 45/74 (60.81%), Postives = 57/74 (77.03%), Query Frame = -2 
Query:  110 MRLTLEFSGGAELLVGKIKKHEVNVPDNVKI--MKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSL 325
            + + +EF GGAELL   IKKH V +P   +   +++LL+WIK +L+KERPELF+QGDSVRPGILVLIND DW L
Sbjct:    5 LSVEVEFGGGAELLFDGIKKHRVTLPGQEEPWDIRNLLIWIKKNLLKERPELFIQGDSVRPGILVLINDADWEL 78
[Close]
BLAST of SMED30033876 vs. Ensembl Human
Match: URM1 (ubiquitin related modifier 1 [Source:HGNC Symbol;Acc:HGNC:28378])

HSP 1 Score: 63.929 bits (154), Expect = 2.437e-14
Identity = 32/59 (54.24%), Postives = 44/59 (74.58%), Query Frame = -2 
Query:  155 MRLTLEFSGGAELLVGKIKKHEVNVPDNVKI--MKDLLLWIKSSLIKERPELFLQGDSV 325
            + + +EF GGAELL   IKKH V +P   +   +++LL+WIK +L+KERPELF+QGDSV
Sbjct:    5 LSVEVEFGGGAELLFDGIKKHRVTLPGQEEPWDIRNLLIWIKKNLLKERPELFIQGDSV 63
[Close]
BLAST of SMED30033876 vs. Ensembl Celegans
Match: urm-1 (Ubiquitin-related modifier 1 homolog [Source:UniProtKB/Swiss-Prot;Acc:B3CKG1])

HSP 1 Score: 65.4698 bits (158), Expect = 6.168e-15
Identity = 39/97 (40.21%), Postives = 60/97 (61.86%), Query Frame = -2 
Query:   41 LTLEFSGGAELLVGKIKKHEVNVPDNVKIMKDLLLWIKSSLIKE--RPELFLQGDS--VRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 319
            +T++FSGGAE LV K K  +V +P +   ++D+L +++ +L+ +  R  + L  D+  V  G++ LIND D  L  E   ++   DTI FVSTLHGG
Sbjct:    6 VTIDFSGGAEFLV-KAKAQKVQIPAD-STLRDVLKFVRDNLVTDVHRINMLLNDDASEVAHGVITLINDTDTGLLLEYDTVIEAGDTITFVSTLHGG 100
[Close]
BLAST of SMED30033876 vs. Ensembl Fly
Match: Urm1 (gene:FBgn0053276 transcript:FBtr0114530)

HSP 1 Score: 112.849 bits (281), Expect = 1.367e-33
Identity = 55/96 (57.29%), Postives = 72/96 (75.00%), Query Frame = -2 
Query:   41 MRLTLEFSGGAELLVGKIKKHEVNVPDNVK-IMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 325
            +++ LEFS GAELL G IK+ E+N+    K  + +LL W+ ++++ ERPELFLQGD+VRPGILVLIND DW L  E+ Y L  ND +LF+STLHGG
Sbjct:    6 LKIILEFSAGAELLFGNIKRRELNLDGKQKWTIANLLKWMHANILTERPELFLQGDTVRPGILVLINDTDWELLGELDYELQPNDNVLFISTLHGG 101
[Close]
BLAST of SMED30033876 vs. Ensembl Zebrafish
Match: urm1 (ubiquitin related modifier 1 [Source:ZFIN;Acc:ZDB-GENE-091204-300])

HSP 1 Score: 118.627 bits (296), Expect = 1.305e-35
Identity = 57/95 (60.00%), Postives = 70/95 (73.68%), Query Frame = -2 
Query:   41 LTLEFSGGAELLVGKIKKHEVNVPD--NVKIMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 319
            L LEF GGAELL   +K H V +PD  N   MK LL WI+ +++KERPELF+QGD+VRPGILVLIND DW L  E+ Y L + D ++F+STLHGG
Sbjct:    7 LHLEFGGGAELLFDGVKNHHVTLPDQSNPWDMKQLLAWIRGNMLKERPELFMQGDTVRPGILVLINDADWELMGELDYQLQDKDNVVFISTLHGG 101
[Close]
BLAST of SMED30033876 vs. Ensembl Zebrafish
Match: urm1 (ubiquitin related modifier 1 [Source:ZFIN;Acc:ZDB-GENE-091204-300])

HSP 1 Score: 118.627 bits (296), Expect = 1.305e-35
Identity = 57/95 (60.00%), Postives = 70/95 (73.68%), Query Frame = -2 
Query:   41 LTLEFSGGAELLVGKIKKHEVNVPD--NVKIMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 319
            L LEF GGAELL   +K H V +PD  N   MK LL WI+ +++KERPELF+QGD+VRPGILVLIND DW L  E+ Y L + D ++F+STLHGG
Sbjct:    7 LHLEFGGGAELLFDGVKNHHVTLPDQSNPWDMKQLLAWIRGNMLKERPELFMQGDTVRPGILVLINDADWELMGELDYQLQDKDNVVFISTLHGG 101
[Close]
BLAST of SMED30033876 vs. Ensembl Zebrafish
Match: urm1 (ubiquitin related modifier 1 [Source:ZFIN;Acc:ZDB-GENE-091204-300])

HSP 1 Score: 111.309 bits (277), Expect = 9.398e-33
Identity = 56/95 (58.95%), Postives = 69/95 (72.63%), Query Frame = -2 
Query:   41 LTLEFSGGAELLVGKIKKHEVNVPD--NVKIMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 319
            L LEF GGAELL   +K H V +PD  N   MK LL WI+ +++KERPELF+QGD+V PGILVLIND DW L  E+ Y L + D ++F+STLHGG
Sbjct:    7 LHLEFGGGAELLFDGVKNHHVTLPDQSNPWDMKQLLAWIRGNMLKERPELFMQGDTV-PGILVLINDADWELMGELDYQLQDKDNVVFISTLHGG 100
[Close]
BLAST of SMED30033876 vs. Ensembl Xenopus
Match: ENSXETT00000045589.1 (ubiquitin related modifier 1 [Source:NCBI gene;Acc:100379922])

HSP 1 Score: 117.087 bits (292), Expect = 6.692e-35
Identity = 57/98 (58.16%), Postives = 75/98 (76.53%), Query Frame = -2 
Query:   41 IMRLTLEFSGGAELLVGKIKKHEVNVPD--NVKIMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 328
            ++ L LEF GGAELL   IK+H+V +P+  N   ++ LL+WI+ +L+KERPELF+QGD+VRPGILVLIND DW L  E+ Y L + D I+F+STLHGG
Sbjct:    4 LVSLLLEFGGGAELLFDGIKQHQVTLPNQSNPWDIRQLLVWIRHNLLKERPELFIQGDTVRPGILVLINDADWELMGELDYQLEDKDNIVFISTLHGG 101
[Close]
BLAST of SMED30033876 vs. Ensembl Xenopus
Match: ENSXETT00000045584.1 (ubiquitin related modifier 1 [Source:NCBI gene;Acc:100379922])

HSP 1 Score: 112.464 bits (280), Expect = 2.329e-32
Identity = 54/91 (59.34%), Postives = 70/91 (76.92%), Query Frame = -2 
Query:   41 FSGGAELLVGKIKKHEVNVPD--NVKIMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 307
            F GGAELL   IK+H+V +P+  N   ++ LL+WI+ +L+KERPELF+QGD+VRPGILVLIND DW L  E+ Y L + D I+F+STLHGG
Sbjct:   65 FLGGAELLFDGIKQHQVTLPNQSNPWDIRQLLVWIRHNLLKERPELFIQGDTVRPGILVLINDADWELMGELDYQLEDKDNIVFISTLHGG 155
[Close]
BLAST of SMED30033876 vs. Ensembl Mouse
Match: Urm1 (ubiquitin related modifier 1 [Source:MGI Symbol;Acc:MGI:1915455])

HSP 1 Score: 117.087 bits (292), Expect = 4.798e-35
Identity = 57/97 (58.76%), Postives = 75/97 (77.32%), Query Frame = -2 
Query:   41 MRLTLEFSGGAELLVGKIKKHEVNVPDNVKI--MKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 325
            + + +EF GGAELL   +KKH+V +P   +   +++LL+WIK +L+KERPELF+QGDSVRPGILVLIND DW L  E+ Y L + D+ILF+STLHGG
Sbjct:    5 LCVKVEFGGGAELLFDGVKKHQVALPGQEEPWDIRNLLVWIKKNLLKERPELFIQGDSVRPGILVLINDADWELLGELDYQLQDQDSILFISTLHGG 101
[Close]
BLAST of SMED30033876 vs. UniProt/SwissProt
Match: sp|Q9BTM9|URM1_HUMAN (Ubiquitin-related modifier 1 OS=Homo sapiens OX=9606 GN=URM1 PE=1 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 1.879e-34
Identity = 57/97 (58.76%), Postives = 74/97 (76.29%), Query Frame = -2 
Query:   41 MRLTLEFSGGAELLVGKIKKHEVNVPDNVKI--MKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 325
            + + +EF GGAELL   IKKH V +P   +   +++LL+WIK +L+KERPELF+QGDSVRPGILVLIND DW L  E+ Y L + D++LF+STLHGG
Sbjct:    5 LSVEVEFGGGAELLFDGIKKHRVTLPGQEEPWDIRNLLIWIKKNLLKERPELFIQGDSVRPGILVLINDADWELLGELDYQLQDQDSVLFISTLHGG 101
[Close]
BLAST of SMED30033876 vs. UniProt/SwissProt
Match: sp|Q9D2P4|URM1_MOUSE (Ubiquitin-related modifier 1 OS=Mus musculus OX=10090 GN=Urm1 PE=1 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 3.356e-34
Identity = 57/97 (58.76%), Postives = 75/97 (77.32%), Query Frame = -2 
Query:   41 MRLTLEFSGGAELLVGKIKKHEVNVPDNVKI--MKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 325
            + + +EF GGAELL   +KKH+V +P   +   +++LL+WIK +L+KERPELF+QGDSVRPGILVLIND DW L  E+ Y L + D+ILF+STLHGG
Sbjct:    5 LCVKVEFGGGAELLFDGVKKHQVALPGQEEPWDIRNLLVWIKKNLLKERPELFIQGDSVRPGILVLINDADWELLGELDYQLQDQDSILFISTLHGG 101
[Close]
BLAST of SMED30033876 vs. UniProt/SwissProt
Match: sp|A9YUB5|URM1_CAPHI (Ubiquitin-related modifier 1 OS=Capra hircus OX=9925 GN=URM1 PE=3 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 3.827e-34
Identity = 55/97 (56.70%), Postives = 74/97 (76.29%), Query Frame = -2 
Query:   41 MRLTLEFSGGAELLVGKIKKHEVNVPDNVKI--MKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 325
            + + +EF GGAELL   +KKH+V +P   +   ++ LL+WIK +L+KERPELF+QGDSVRPGILVL+ND DW L  E+ Y L + D++LF+STLHGG
Sbjct:    5 LSVEVEFGGGAELLFDGVKKHQVTLPGQEEPWDIRSLLVWIKKNLLKERPELFIQGDSVRPGILVLVNDADWELLGELDYQLQDQDSVLFISTLHGG 101
[Close]
BLAST of SMED30033876 vs. UniProt/SwissProt
Match: sp|Q148F0|URM1_BOVIN (Ubiquitin-related modifier 1 OS=Bos taurus OX=9913 GN=URM1 PE=3 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 3.827e-34
Identity = 55/97 (56.70%), Postives = 74/97 (76.29%), Query Frame = -2 
Query:   41 MRLTLEFSGGAELLVGKIKKHEVNVPDNVKI--MKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 325
            + + +EF GGAELL   +KKH+V +P   +   ++ LL+WIK +L+KERPELF+QGDSVRPGILVL+ND DW L  E+ Y L + D++LF+STLHGG
Sbjct:    5 LSVEVEFGGGAELLFDGVKKHQVTLPGQEEPWDIRSLLVWIKKNLLKERPELFIQGDSVRPGILVLVNDADWELLGELDYQLQDQDSVLFISTLHGG 101
[Close]
BLAST of SMED30033876 vs. UniProt/SwissProt
Match: sp|Q5ZJU4|URM1_CHICK (Ubiquitin-related modifier 1 OS=Gallus gallus OX=9031 GN=URM1 PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 7.795e-34
Identity = 56/95 (58.95%), Postives = 72/95 (75.79%), Query Frame = -2 
Query:   41 LTLEFSGGAELLVGKIKKHEVNVPDNVKI--MKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 319
            L +EF GGAELL   +KKH+V +P   +   +++LL WIK +L+KERPELF+QG+SVRPGILVLIND DW L  E+ Y L + D +LF+STLHGG
Sbjct:    7 LQVEFGGGAELLFDGVKKHQVTLPSQPEPWDIRNLLKWIKQNLLKERPELFMQGESVRPGILVLINDADWELMGELDYKLQDQDNVLFISTLHGG 101
[Close]
BLAST of SMED30033876 vs. TrEMBL
Match: A0A5J4NC37 (Ubiquitin-related modifier 1 homolog OS=Paragonimus westermani OX=34504 GN=DEA37_0003254 PE=3 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 1.593e-36
Identity = 63/97 (64.95%), Postives = 79/97 (81.44%), Query Frame = -2 
Query:   41 FIMRLTLEFSGGAELLVGKIKKHEVNVPDNVKIMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 331
            F M++ LEFSGG+ELL GK+K+HEV+VP NV  +KDLL WI+ +L++ERPELFLQG+ +RPGILVLIND D+SL  E  Y L E+D I FVS+LHGG
Sbjct:  150 FPMKIHLEFSGGSELLFGKVKEHEVDVPSNVVHLKDLLSWIRDNLLQERPELFLQGNRIRPGILVLINDTDYSLLGEDDYPLCESDRIAFVSSLHGG 246
[Close]
BLAST of SMED30033876 vs. TrEMBL
Match: A0A1I8G7D7 (Ubiquitin-related modifier 1 homolog OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 8.678e-35
Identity = 58/96 (60.42%), Postives = 75/96 (78.12%), Query Frame = -2 
Query:   41 MRLTLEFSGGAELLVGKIKKHEVNVPDNVK-IMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 325
            M+LTLEF GGAE LVG +KKH +++PD  +  +  LL WIK++ ++ERPELFLQG SVRPGILVL+ND DW L  E+ Y L +ND ++F+STLHGG
Sbjct:    1 MKLTLEFGGGAEFLVGNVKKHAIDLPDTQQWTLGQLLEWIKANKLQERPELFLQGSSVRPGILVLVNDTDWELLGELSYQLKDNDDVVFISTLHGG 96
[Close]
BLAST of SMED30033876 vs. TrEMBL
Match: A0A267GU21 (Ubiquitin-related modifier 1 (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027905g1 PE=3 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 1.387e-34
Identity = 58/96 (60.42%), Postives = 75/96 (78.12%), Query Frame = -2 
Query:   41 MRLTLEFSGGAELLVGKIKKHEVNVPDNVK-IMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 325
            M+LTLEF GGAE LVG +KKH +++PD  +  +  LL WIK++ ++ERPELFLQG SVRPGILVL+ND DW L  E+ Y L +ND ++F+STLHGG
Sbjct:    5 MKLTLEFGGGAEFLVGNVKKHTIDLPDTQQWTLGQLLEWIKANKLQERPELFLQGSSVRPGILVLVNDTDWELLGELSYQLKDNDDVVFISTLHGG 100
[Close]
BLAST of SMED30033876 vs. TrEMBL
Match: A0A0R3U8I2 (Ubiquitin-related modifier 1 homolog OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS3189 PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 1.539e-34
Identity = 58/95 (61.05%), Postives = 75/95 (78.95%), Query Frame = -2 
Query:   41 MRLTLEFSGGAELLVGKIKKHEVNVPDNVKIMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 325
            MR+ LEFSGGAELL G  ++ EV++PD+V  +K LL W++ +L+K RP+LF+Q +S+RPGIL+LIND DWSL NE +Y L E D I FVSTLHGG
Sbjct:    1 MRIFLEFSGGAELLFGGKREQEVDLPDSVTTIKHLLPWLRDNLLKGRPDLFMQSNSIRPGILILINDTDWSLHNEEEYQLKEKDKISFVSTLHGG 95
[Close]
BLAST of SMED30033876 vs. TrEMBL
Match: A0A139WLE8 (Ubiquitin-related modifier 1 homolog OS=Tribolium castaneum OX=7070 GN=TcasGA2_TC032387 PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 1.821e-34
Identity = 61/97 (62.89%), Postives = 76/97 (78.35%), Query Frame = -2 
Query:   41 MRLTLEFSGGAELLVGKIKKHEVNVPDNVK--IMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 325
            M +T+EF GGAELL    K H+V +P+  K  I+KDLL+WIK +L+KERPELFLQ +SVRPGILVLIND DW L + + Y++ END ILF+STLHGG
Sbjct:    1 MHITVEFGGGAELLFDNKKSHDVTLPEKDKEWIIKDLLIWIKDNLLKERPELFLQDESVRPGILVLINDADWELMDTVNYVIQENDRILFISTLHGG 97
[Close]
BLAST of SMED30033876 vs. Ensembl Cavefish
Match: urm1 (ubiquitin related modifier 1 [Source:ZFIN;Acc:ZDB-GENE-091204-300])

HSP 1 Score: 95.1301 bits (235), Expect = 1.685e-26
Identity = 42/64 (65.62%), Postives = 53/64 (82.81%), Query Frame = -2 
Query:   41 MKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 232
            MK LL WI+ +L+KERPELF+QGD+VRPGILVLIND DW L  E++Y L + D ++F+STLHGG
Sbjct:   33 MKQLLAWIRVNLLKERPELFVQGDTVRPGILVLINDADWELMGELEYQLQDQDNVVFISTLHGG 96
[Close]
BLAST of SMED30033876 vs. Ensembl Sea Lamprey
Match: urm1 (ubiquitin related modifier 1 [Source:ZFIN;Acc:ZDB-GENE-091204-300])

HSP 1 Score: 116.316 bits (290), Expect = 1.365e-35
Identity = 56/97 (57.73%), Postives = 74/97 (76.29%), Query Frame = -2 
Query:   41 MRLTLEFSGGAELLVGKIKKHEVNVPDNVKI--MKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 325
            + L +EF GGAELL G +K H+V +    K   ++ LL+WI+ +L+KERPELF+QGD+VRPGILVLIND DW L  E+ Y LV+ DT++F+STLHGG
Sbjct:    5 ISLNVEFGGGAELLFGGVKSHKVELSQQEKPWDVRQLLVWIRKNLLKERPELFIQGDNVRPGILVLINDTDWELLGELDYKLVDQDTVVFISTLHGG 101
[Close]
BLAST of SMED30033876 vs. Ensembl Yeast
Match: URM1 (Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response [Source:SGD;Acc:S000001270])

HSP 1 Score: 73.1738 bits (178), Expect = 9.172e-19
Identity = 39/99 (39.39%), Postives = 63/99 (63.64%), Query Frame = -2 
Query:   41 IMRLTLEFSGGAELLVGKIKKHEVNV-PDNVKIMKDLLLWIKSSLIKERPE--LFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 328
            ++ + +EF GG + + GK + H++ +  ++   + DL+  I S++I    +  +F++ DS+RPGI+ LIND DW LE E  Y+L + D I F STLHGG
Sbjct:    1 MVNVKVEFLGGLDAIFGKQRVHKIKMDKEDPVTVGDLIDHIVSTMINNPNDVSIFIEDDSIRPGIITLINDTDWELEGEKDYILEDGDIISFTSTLHGG 99
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BLAST of SMED30033876 vs. Ensembl Nematostella
Match: EDO33943 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQT3])

HSP 1 Score: 48.9062 bits (115), Expect = 2.789e-9
Identity = 25/50 (50.00%), Postives = 36/50 (72.00%), Query Frame = -2 
Query:  155 GAELLVGKIKKHEVNVPDNVKI--MKDLLLWIKSSLIKERPELFLQGDSV 298
            GAELL   +KKH+V +  +     +K LL WI+ +L+KERP+LF+QG+SV
Sbjct:    2 GAELLFDNVKKHDVQLARDQTPWNIKRLLPWIRDNLLKERPDLFMQGESV 51
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BLAST of SMED30033876 vs. Ensembl Medaka
Match: urm1 (ubiquitin related modifier 1 [Source:NCBI gene;Acc:110014343])

HSP 1 Score: 115.161 bits (287), Expect = 2.110e-34
Identity = 55/93 (59.14%), Postives = 70/93 (75.27%), Query Frame = -2 
Query:   41 LEFSGGAELLVGKIKKHEVNVPDNVKI--MKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 313
            LEF GGAELL   +K+H V +P   +   M+ LL+WI+ +L+KERPELF+QGDSVRPGILVLIND DW L  E+ Y L + D ++F+STLHGG
Sbjct:    9 LEFGGGAELLFDGVKEHHVTLPSQPEPWGMRQLLVWIQRNLLKERPELFVQGDSVRPGILVLINDADWELMGELDYQLQDQDNVVFISTLHGG 101
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BLAST of SMED30033876 vs. Planmine SMEST
Match: SMESG000064827.1 (SMESG000064827.1)

HSP 1 Score: 189.504 bits (480), Expect = 5.403e-64
Identity = 95/95 (100.00%), Postives = 95/95 (100.00%), Query Frame = -2 
Query:   41 MRLTLEFSGGAELLVGKIKKHEVNVPDNVKIMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 325
            MRLTLEFSGGAELLVGKIKKHEVNVPDNVKIMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG
Sbjct:    1 MRLTLEFSGGAELLVGKIKKHEVNVPDNVKIMKDLLLWIKSSLIKERPELFLQGDSVRPGILVLINDVDWSLENEIQYMLVENDTILFVSTLHGG 95
The following BLAST results are available for this feature:
BLAST of SMED30033876 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
URM13.913e-3558.76ubiquitin related modifier 1 [Source:HGNC Symbol;A... [more]
URM15.819e-2460.81ubiquitin related modifier 1 [Source:HGNC Symbol;A... [more]
URM12.437e-1454.24ubiquitin related modifier 1 [Source:HGNC Symbol;A... [more]
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BLAST of SMED30033876 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
urm-16.168e-1540.21Ubiquitin-related modifier 1 homolog [Source:UniP... [more]
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BLAST of SMED30033876 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Urm11.367e-3357.29gene:FBgn0053276 transcript:FBtr0114530[more]
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BLAST of SMED30033876 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
urm11.305e-3560.00ubiquitin related modifier 1 [Source:ZFIN;Acc:ZDB-... [more]
urm11.305e-3560.00ubiquitin related modifier 1 [Source:ZFIN;Acc:ZDB-... [more]
urm19.398e-3358.95ubiquitin related modifier 1 [Source:ZFIN;Acc:ZDB-... [more]
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BLAST of SMED30033876 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSXETT00000045589.16.692e-3558.16ubiquitin related modifier 1 [Source:NCBI gene;Acc... [more]
ENSXETT00000045584.12.329e-3259.34ubiquitin related modifier 1 [Source:NCBI gene;Acc... [more]
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BLAST of SMED30033876 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Urm14.798e-3558.76ubiquitin related modifier 1 [Source:MGI Symbol;Ac... [more]
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BLAST of SMED30033876 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9BTM9|URM1_HUMAN1.879e-3458.76Ubiquitin-related modifier 1 OS=Homo sapiens OX=96... [more]
sp|Q9D2P4|URM1_MOUSE3.356e-3458.76Ubiquitin-related modifier 1 OS=Mus musculus OX=10... [more]
sp|A9YUB5|URM1_CAPHI3.827e-3456.70Ubiquitin-related modifier 1 OS=Capra hircus OX=99... [more]
sp|Q148F0|URM1_BOVIN3.827e-3456.70Ubiquitin-related modifier 1 OS=Bos taurus OX=9913... [more]
sp|Q5ZJU4|URM1_CHICK7.795e-3458.95Ubiquitin-related modifier 1 OS=Gallus gallus OX=9... [more]
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BLAST of SMED30033876 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4NC371.593e-3664.95Ubiquitin-related modifier 1 homolog OS=Paragonimu... [more]
A0A1I8G7D78.678e-3560.42Ubiquitin-related modifier 1 homolog OS=Macrostomu... [more]
A0A267GU211.387e-3460.42Ubiquitin-related modifier 1 (Fragment) OS=Macrost... [more]
A0A0R3U8I21.539e-3461.05Ubiquitin-related modifier 1 homolog OS=Mesocestoi... [more]
A0A139WLE81.821e-3462.89Ubiquitin-related modifier 1 homolog OS=Tribolium ... [more]
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BLAST of SMED30033876 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
urm11.685e-2665.63ubiquitin related modifier 1 [Source:ZFIN;Acc:ZDB-... [more]
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BLAST of SMED30033876 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
urm11.365e-3557.73ubiquitin related modifier 1 [Source:ZFIN;Acc:ZDB-... [more]
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BLAST of SMED30033876 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
URM19.172e-1939.39Ubiquitin-like protein involved in thiolation of c... [more]
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BLAST of SMED30033876 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO339432.789e-950.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30033876 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
urm12.110e-3459.14ubiquitin related modifier 1 [Source:NCBI gene;Acc... [more]
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BLAST of SMED30033876 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000064827.15.403e-64100.00SMESG000064827.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033876 ID=SMED30033876|Name=SMED30033876|organism=Schmidtea mediterranea sexual|type=transcript|length=350bp
AACTCTTTTAAAATATATATTAATGAAAACATTTTAACTATCCACCATGT
AAAGTTGAAACAAACAATATTGTATCATTTTCAACTAGCATATATTGAAT
TTCATTTTCCAAACTCCAATCAACATCGTTTATTAATACTAATATTCCTG
GTCTAACACTATCTCCTTGCAGAAATAATTCTGGTCTTTCTTTAATAAGG
CTGCTCTTAATCCATAACAGCAAATCTTTCATAATTTTTACATTATCTGG
CACATTTACTTCATGTTTTTTAATTTTACCTACAAGAAGCTCAGCTCCAC
CTGAAAACTCTAATGTTAGACGCATGATAAATTATTAAATAAAAATCAAA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR012675Beta-grasp_dom_sf
IPR015221Urm1
IPR016155Mopterin_synth/thiamin_S_b
Vocabulary: biological process
TermDefinition
GO:0034227tRNA thio-modification
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015221Ubiquitin-related modifier 1PFAMPF09138Urm1coord: 3..95
e-value: 2.0E-33
score: 114.6
IPR015221Ubiquitin-related modifier 1PIRSFPIRSF037379Urm1coord: 1..95
e-value: 2.8E-36
score: 121.5
IPR015221Ubiquitin-related modifier 1PANTHERPTHR14986RURM1 PROTEINcoord: 1..95
IPR015221Ubiquitin-related modifier 1CDDcd01764Ubl_Urm1coord: 3..95
e-value: 2.76375E-45
score: 138.813
IPR012675Beta-grasp domain superfamilyGENE3DG3DSA:3.10.20.30coord: 1..95
e-value: 8.6E-35
score: 121.2
IPR016155Molybdopterin synthase/thiamin biosynthesis sulphur carrier, beta-graspSUPERFAMILYSSF54285MoaD/ThiScoord: 1..95