G_PROTEIN_RECEP_F3_4 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F3_4 domain-containing protein
Smed IDSMED30033858
Length (bp)420
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F3_4 domain-containing protein (SMED30033858) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F3_4 domain-containing protein (SMED30033858) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30033858

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30033858h1SMcG0010057 dd_Smed_v6_77671_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
oocyteSMED30033858h1SMcG0010057 Contig9714uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30033858h1SMcG0010057 Contig9714newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30033858h1SMcG0010057 Contig9714uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30033858h1SMcG0010057 Contig9714newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Human
Match: GRM5 (glutamate metabotropic receptor 5 [Source:HGNC Symbol;Acc:HGNC:4597])

HSP 1 Score: 56.225 bits (134), Expect = 1.620e-9
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  729 VYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 832          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Human
Match: GRM5 (glutamate metabotropic receptor 5 [Source:HGNC Symbol;Acc:HGNC:4597])

HSP 1 Score: 56.225 bits (134), Expect = 1.815e-9
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  729 VYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 832          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Human
Match: GRM5 (glutamate metabotropic receptor 5 [Source:HGNC Symbol;Acc:HGNC:4597])

HSP 1 Score: 56.225 bits (134), Expect = 1.815e-9
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  729 VYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 832          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Human
Match: GRM1 (glutamate metabotropic receptor 1 [Source:HGNC Symbol;Acc:HGNC:4593])

HSP 1 Score: 55.8398 bits (133), Expect = 2.380e-9
Identity = 36/108 (33.33%), Postives = 61/108 (56.48%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L ++  TY+ FKTR +P NF+++K I      +  +WL+ +P    ++  +I +    FAV ++ T+ LG +F PK+Y + I K E N
Sbjct:  742 VYLICNTSNLGVVAPLGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTC---FAVSLSVTVALGCMFTPKMY-IIIAKPERN 845          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Human
Match: GRM1 (glutamate metabotropic receptor 1 [Source:HGNC Symbol;Acc:HGNC:4593])

HSP 1 Score: 55.8398 bits (133), Expect = 2.380e-9
Identity = 36/108 (33.33%), Postives = 61/108 (56.48%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L ++  TY+ FKTR +P NF+++K I      +  +WL+ +P    ++  +I +    FAV ++ T+ LG +F PK+Y + I K E N
Sbjct:  742 VYLICNTSNLGVVAPLGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTC---FAVSLSVTVALGCMFTPKMY-IIIAKPERN 845          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Celegans
Match: mgl-2 (Metabotropic GLutamate receptor family [Source:UniProtKB/TrEMBL;Acc:A0A0K3AU50])

HSP 1 Score: 50.0618 bits (118), Expect = 9.522e-8
Identity = 30/103 (29.13%), Postives = 60/103 (58.25%), Query Frame = 1
Query:   16 CNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALIL-FAVFVNSTLVLGTLFIPKIYALFILKEE 321
            C+ +   F IP  W++  + + T + FKTR LPENF+++K I     C++ +W++ +   + T+      AL++ F+  +++++ L  LF PK+Y + +  E+
Sbjct:  732 CDTETKSFLIPFFWDFFLITLCTLYAFKTRNLPENFNEAKFIGFTMYCTVVVWIAFLVLHMGTT----HKALVMSFSYSLSASVALALLFFPKLYIILMHPEK 830          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Celegans
Match: mgl-2 (Metabotropic GLutamate receptor family [Source:UniProtKB/TrEMBL;Acc:A0A0K3AU50])

HSP 1 Score: 50.0618 bits (118), Expect = 1.013e-7
Identity = 30/103 (29.13%), Postives = 60/103 (58.25%), Query Frame = 1
Query:   16 CNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALIL-FAVFVNSTLVLGTLFIPKIYALFILKEE 321
            C+ +   F IP  W++  + + T + FKTR LPENF+++K I     C++ +W++ +   + T+      AL++ F+  +++++ L  LF PK+Y + +  E+
Sbjct:  732 CDTETKSFLIPFFWDFFLITLCTLYAFKTRNLPENFNEAKFIGFTMYCTVVVWIAFLVLHMGTT----HKALVMSFSYSLSASVALALLFFPKLYIILMHPEK 830          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Celegans
Match: mgl-2 (Metabotropic GLutamate receptor family [Source:UniProtKB/TrEMBL;Acc:A0A0K3AU50])

HSP 1 Score: 49.6766 bits (117), Expect = 1.170e-7
Identity = 30/103 (29.13%), Postives = 60/103 (58.25%), Query Frame = 1
Query:   16 CNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALIL-FAVFVNSTLVLGTLFIPKIYALFILKEE 321
            C+ +   F IP  W++  + + T + FKTR LPENF+++K I     C++ +W++ +   + T+      AL++ F+  +++++ L  LF PK+Y + +  E+
Sbjct:  732 CDTETKSFLIPFFWDFFLITLCTLYAFKTRNLPENFNEAKFIGFTMYCTVVVWIAFLVLHMGTT----HKALVMSFSYSLSASVALALLFFPKLYIILMHPEK 830          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Celegans
Match: mgl-3 (Metabotropic GLutamate receptor family [Source:UniProtKB/TrEMBL;Acc:A0A0K3AUI1])

HSP 1 Score: 47.3654 bits (111), Expect = 8.860e-7
Identity = 31/97 (31.96%), Postives = 50/97 (51.55%), Query Frame = 1
Query:   10 MVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSS-FLIQSALILFAVFVNSTLVLGTLFIPKIY 297
            + C        I + +N L ++  T + FKTRK+PENF++++ I      +  LWL+  P   AT S F IQ   +   + ++ T+ L   F PK+Y
Sbjct:  725 LTCKATTSHLLISLLYNILLIVACTVYAFKTRKIPENFNETRHIGFTMYSTCILWLAFGPIYFATQSDFRIQITSLCMCISLSGTVALICFFAPKVY 821          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Celegans
Match: mgl-3 (Metabotropic GLutamate receptor family [Source:UniProtKB/TrEMBL;Acc:A0A0K3AUI1])

HSP 1 Score: 47.3654 bits (111), Expect = 8.860e-7
Identity = 31/97 (31.96%), Postives = 50/97 (51.55%), Query Frame = 1
Query:   10 MVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSS-FLIQSALILFAVFVNSTLVLGTLFIPKIY 297
            + C        I + +N L ++  T + FKTRK+PENF++++ I      +  LWL+  P   AT S F IQ   +   + ++ T+ L   F PK+Y
Sbjct:  725 LTCKATTSHLLISLLYNILLIVACTVYAFKTRKIPENFNETRHIGFTMYSTCILWLAFGPIYFATQSDFRIQITSLCMCISLSGTVALICFFAPKVY 821          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Zebrafish
Match: grm1a (glutamate receptor, metabotropic 1a [Source:ZFIN;Acc:ZDB-GENE-030131-7893])

HSP 1 Score: 56.6102 bits (135), Expect = 8.145e-10
Identity = 35/108 (32.41%), Postives = 62/108 (57.41%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L ++  TY+ FKTR +P NF+++K I      +  +WL+ +P    ++  +I ++   F+V ++ T+ LG +F PK+Y + I K E N
Sbjct:  747 VYLICNTSNVGVVAPLGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTS---FSVSLSVTVALGCMFTPKMY-IIIAKPERN 850          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Zebrafish
Match: grm1a (glutamate receptor, metabotropic 1a [Source:ZFIN;Acc:ZDB-GENE-030131-7893])

HSP 1 Score: 56.6102 bits (135), Expect = 8.149e-10
Identity = 35/108 (32.41%), Postives = 62/108 (57.41%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L ++  TY+ FKTR +P NF+++K I      +  +WL+ +P    ++  +I ++   F+V ++ T+ LG +F PK+Y + I K E N
Sbjct:  755 VYLICNTSNVGVVAPLGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTS---FSVSLSVTVALGCMFTPKMY-IIIAKPERN 858          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Zebrafish
Match: grm1b (glutamate receptor, metabotropic 1b [Source:ZFIN;Acc:ZDB-GENE-090821-3])

HSP 1 Score: 56.225 bits (134), Expect = 1.142e-9
Identity = 36/108 (33.33%), Postives = 62/108 (57.41%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  TY+ FKTR +P NF+++K I      +  +WL+ +P    ++  +I ++   F+V ++ T+ LG +F PK+Y + I K E N
Sbjct:  742 VFLICNTSNMGMVAPLGYNGLLILSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTS---FSVSLSVTVALGCMFTPKMY-IIIAKPERN 845          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Zebrafish
Match: grm1b (glutamate receptor, metabotropic 1b [Source:ZFIN;Acc:ZDB-GENE-090821-3])

HSP 1 Score: 56.225 bits (134), Expect = 1.142e-9
Identity = 36/108 (33.33%), Postives = 62/108 (57.41%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  TY+ FKTR +P NF+++K I      +  +WL+ +P    ++  +I ++   F+V ++ T+ LG +F PK+Y + I K E N
Sbjct:  742 VFLICNTSNMGMVAPLGYNGLLILSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTS---FSVSLSVTVALGCMFTPKMY-IIIAKPERN 845          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Zebrafish
Match: grm5b (glutamate receptor, metabotropic 5b [Source:ZFIN;Acc:ZDB-GENE-090821-6])

HSP 1 Score: 55.4546 bits (132), Expect = 1.872e-9
Identity = 32/108 (29.63%), Postives = 63/108 (58.33%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L ++  T++ FKTR +P NF+++K I      +  +WL+ +P    ++    ++  + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  733 VNLICNTTNLGVVAPLGYNGLLIMSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSN---YKTITMCFSVSLSATVALGCMFVPKVY-IILAKPERN 836          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Xenopus
Match: GRM5 (glutamate receptor, metabotropic 5 [Source:NCBI gene;Acc:100485273])

HSP 1 Score: 57.3806 bits (137), Expect = 6.774e-10
Identity = 34/108 (31.48%), Postives = 64/108 (59.26%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN +      P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  735 VYLICNTNNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 838          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Xenopus
Match: GRM1 (glutamate receptor, metabotropic 1 [Source:NCBI gene;Acc:100495945])

HSP 1 Score: 57.3806 bits (137), Expect = 6.901e-10
Identity = 36/108 (33.33%), Postives = 62/108 (57.41%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L ++  TY+ FKTR +P NF+++K I      +  +WL+ +P    ++  +I ++   FAV ++ T+ LG +F PK+Y + I K E N
Sbjct:  740 VYLICNTSNLGVVAPLGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTS---FAVSLSVTVALGCMFTPKMY-IIIAKPERN 843          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Xenopus
Match: GRM5 (glutamate receptor, metabotropic 5 [Source:NCBI gene;Acc:100485273])

HSP 1 Score: 57.3806 bits (137), Expect = 7.029e-10
Identity = 34/108 (31.48%), Postives = 64/108 (59.26%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN +      P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  724 VYLICNTNNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 827          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Xenopus
Match: GRM5 (glutamate receptor, metabotropic 5 [Source:NCBI gene;Acc:100485273])

HSP 1 Score: 57.3806 bits (137), Expect = 7.097e-10
Identity = 34/108 (31.48%), Postives = 64/108 (59.26%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN +      P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  735 VYLICNTNNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 838          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Xenopus
Match: GRM5 (glutamate receptor, metabotropic 5 [Source:NCBI gene;Acc:100485273])

HSP 1 Score: 56.9954 bits (136), Expect = 7.453e-10
Identity = 34/108 (31.48%), Postives = 64/108 (59.26%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN +      P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  735 VYLICNTNNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 838          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Mouse
Match: Grm1 (glutamate receptor, metabotropic 1 [Source:MGI Symbol;Acc:MGI:1351338])

HSP 1 Score: 56.9954 bits (136), Expect = 6.971e-10
Identity = 37/108 (34.26%), Postives = 61/108 (56.48%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        PVG+N L ++  TY+ FKTR +P NF+++K I      +  +WL+ +P    ++  +I +    FAV ++ T+ LG +F PK+Y + I K E N
Sbjct:  742 VYLICNTSNLGVVAPVGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTC---FAVSLSVTVALGCMFTPKMY-IIIAKPERN 845          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Mouse
Match: Grm1 (glutamate receptor, metabotropic 1 [Source:MGI Symbol;Acc:MGI:1351338])

HSP 1 Score: 56.9954 bits (136), Expect = 6.971e-10
Identity = 37/108 (34.26%), Postives = 61/108 (56.48%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        PVG+N L ++  TY+ FKTR +P NF+++K I      +  +WL+ +P    ++  +I +    FAV ++ T+ LG +F PK+Y + I K E N
Sbjct:  742 VYLICNTSNLGVVAPVGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTC---FAVSLSVTVALGCMFTPKMY-IIIAKPERN 845          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Mouse
Match: Grm5 (glutamate receptor, metabotropic 5 [Source:MGI Symbol;Acc:MGI:1351342])

HSP 1 Score: 56.6102 bits (135), Expect = 9.528e-10
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  728 VYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 831          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Mouse
Match: Grm5 (glutamate receptor, metabotropic 5 [Source:MGI Symbol;Acc:MGI:1351342])

HSP 1 Score: 56.225 bits (134), Expect = 1.068e-9
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  728 VYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 831          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Mouse
Match: Grm5 (glutamate receptor, metabotropic 5 [Source:MGI Symbol;Acc:MGI:1351342])

HSP 1 Score: 56.225 bits (134), Expect = 1.068e-9
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  728 VYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 831          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. UniProt/SwissProt
Match: sp|P23385|GRM1_RAT (Metabotropic glutamate receptor 1 OS=Rattus norvegicus OX=10116 GN=Grm1 PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 4.318e-9
Identity = 37/108 (34.26%), Postives = 61/108 (56.48%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        PVG+N L ++  TY+ FKTR +P NF+++K I      +  +WL+ +P    ++  +I +    FAV ++ T+ LG +F PK+Y + I K E N
Sbjct:  742 VYLICNTSNLGVVAPVGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTC---FAVSLSVTVALGCMFTPKMY-IIIAKPERN 845          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. UniProt/SwissProt
Match: sp|P31424|GRM5_RAT (Metabotropic glutamate receptor 5 OS=Rattus norvegicus OX=10116 GN=Grm5 PE=1 SV=2)

HSP 1 Score: 56.6102 bits (135), Expect = 6.665e-9
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  728 VYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 831          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q3UVX5|GRM5_MOUSE (Metabotropic glutamate receptor 5 OS=Mus musculus OX=10090 GN=Grm5 PE=1 SV=2)

HSP 1 Score: 56.6102 bits (135), Expect = 6.665e-9
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  728 VYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 831          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. UniProt/SwissProt
Match: sp|P41594|GRM5_HUMAN (Metabotropic glutamate receptor 5 OS=Homo sapiens OX=9606 GN=GRM5 PE=1 SV=2)

HSP 1 Score: 56.225 bits (134), Expect = 7.780e-9
Identity = 34/108 (31.48%), Postives = 63/108 (58.33%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  729 VYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 832          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q13255|GRM1_HUMAN (Metabotropic glutamate receptor 1 OS=Homo sapiens OX=9606 GN=GRM1 PE=1 SV=3)

HSP 1 Score: 55.8398 bits (133), Expect = 1.143e-8
Identity = 36/108 (33.33%), Postives = 61/108 (56.48%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L ++  TY+ FKTR +P NF+++K I      +  +WL+ +P    ++  +I +    FAV ++ T+ LG +F PK+Y + I K E N
Sbjct:  742 VYLICNTSNLGVVAPLGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTC---FAVSLSVTVALGCMFTPKMY-IIIAKPERN 845          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. TrEMBL
Match: A0A1I8JG12 (G_PROTEIN_RECEP_F3_4 domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.760e-9
Identity = 38/107 (35.51%), Postives = 63/107 (58.88%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEEL 324
            VE  C+      WIP   N L +L++     + R LP+NFHDSKCI  F++C+ F+W++ IPS++  +S     AL++F +   +   +  LF+PK+YA+   + +L
Sbjct: 2987 VEFQCDYFSSSLWIPYALNLLLMLVTCSLAVRVRSLPDNFHDSKCILAFTACATFVWIAFIPSLVTMASLTSAGALLVFVLLFTNLFAVSILFLPKVYAVVFNRADL 3093          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. TrEMBL
Match: A0A1I8HVG3 (G_PROTEIN_RECEP_F3_4 domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.224e-9
Identity = 37/107 (34.58%), Postives = 63/107 (58.88%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEEL 324
            VE  C+      WIP   N + +L++     + R LP+NFHDSKCI  F++C+ F+W++ IPS++  +S     AL++F +   +   +  LF+PK+YA+   + +L
Sbjct:  681 VEFQCDYFSSSLWIPYALNLMLMLVTCSLAVRVRSLPDNFHDSKCILAFTACATFVWIAFIPSLVTMASLTSAGALLVFVLLFTNLFAVSILFLPKVYAVVFNRADL 787          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. TrEMBL
Match: A0A1I8HDN7 (G_PROTEIN_RECEP_F3_4 domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.243e-9
Identity = 37/107 (34.58%), Postives = 63/107 (58.88%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEEL 324
            VE  C+      WIP   N + +L++     + R LP+NFHDSKCI  F++C+ F+W++ IPS++  +S     AL++F +   +   +  LF+PK+YA+   + +L
Sbjct: 1298 VEFQCDYFSSSLWIPYALNLMLMLVTCSLAVRVRSLPDNFHDSKCILAFTACATFVWIAFIPSLVTMASLTSAGALLVFVLLFTNLFAVSILFLPKVYAVVFNRADL 1404          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. TrEMBL
Match: A0A0L8FPT1 (G_PROTEIN_RECEP_F3_4 domain-containing protein (Fragment) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22011612mg PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 4.774e-9
Identity = 37/111 (33.33%), Postives = 66/111 (59.46%), Query Frame = 1
Query:    1 YVEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELNYN 333
            +VEM CN       +P+ +N L ++I  Y+ F++RKLP+NF++S+ I      ++ +WLS +P+   TS    +  ++  A+ +N+T+ L  LF+ K+YAL+  + E   N
Sbjct:   26 FVEMYCNMSPEGLILPLSFNLLLVIICAYYAFRSRKLPDNFNESRYIAFCVDTTLLVWLSFLPTYFLTSRAYYKVLIMSSALIINATVTLICLFVTKVYALYKYRSEYQRN 136          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. TrEMBL
Match: W4YWK5 (G_PROTEIN_RECEP_F3_4 domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 6.602e-9
Identity = 43/131 (32.82%), Postives = 72/131 (54.96%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELNYNQTLHNTVEVSAR*KFFSFSET 396
            V ++CN     F  P+G++ L + + T + FKTR LPENF+++K I      +  +WL+ IP    +    ++  ++ FAV +++T+ LG LF PK Y + +LK E N   ++  T     R    SF+E+
Sbjct:  349 VRLICNTTTFGFAAPLGYDALLISLCTLYAFKTRNLPENFNEAKYIAFTMYTTCIVWLAFIPLYFGSD---LKEIVVCFAVTLSATVTLGCLFFPKTY-IILLKPERNRRSSM--TTSNLVRMHVGSFNES 473          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Cavefish
Match: grm1a (glutamate receptor, metabotropic 1a [Source:ZFIN;Acc:ZDB-GENE-030131-7893])

HSP 1 Score: 56.9954 bits (136), Expect = 4.737e-10
Identity = 35/108 (32.41%), Postives = 62/108 (57.41%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L ++  TY+ FKTR +P NF+++K I      +  +WL+ +P    ++  +I ++   F+V ++ T+ LG +F PK+Y + I K E N
Sbjct:  736 VYLICNTSNVGMIAPLGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTS---FSVSLSVTVALGCMFTPKMY-IIIAKPERN 839          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Cavefish
Match: grm1b (glutamate receptor, metabotropic 1b [Source:ZFIN;Acc:ZDB-GENE-090821-3])

HSP 1 Score: 56.225 bits (134), Expect = 1.026e-9
Identity = 36/108 (33.33%), Postives = 62/108 (57.41%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  TY+ FKTR +P NF+++K I      +  +WL+ +P    ++  +I ++   F+V ++ T+ LG +F PK+Y + I K E N
Sbjct:  745 VFLICNTSNMGMVAPLGYNGLLILSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITTS---FSVSLSVTVALGCMFTPKMY-IIIAKPERN 848          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Cavefish
Match: grm2a (metabotropic glutamate receptor 2 [Source:NCBI gene;Acc:103035135])

HSP 1 Score: 55.4546 bits (132), Expect = 1.518e-9
Identity = 31/102 (30.39%), Postives = 59/102 (57.84%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSS-FLIQSALILFAVFVNSTLVLGTLFIPKIYALF 306
            V + CN       + + +N + +++ T++ FKTRK PENF+++K I      +  +WL+  P    T+S + +Q+  +  +V ++ ++VLG LF PKI+ + 
Sbjct:  732 VRLKCNSKDSSMLMSLTYNCILIILCTFYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFQPIFYVTASDYRVQTTTMCISVSLSGSVVLGCLFAPKIHIIL 833          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Cavefish
Match: grm5b (metabotropic glutamate receptor 5 [Source:NCBI gene;Acc:103045948])

HSP 1 Score: 55.4546 bits (132), Expect = 1.528e-9
Identity = 32/108 (29.63%), Postives = 63/108 (58.33%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L ++  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  ++    + F+V +++T+ LG +F+PK+Y + + K E N
Sbjct:  733 VNLICNTTNLGVVAPLGYNGLLIMSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIVT---MCFSVSLSATVALGCMFVPKVY-IILAKPERN 836          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Cavefish
Match: grm2b (metabotropic glutamate receptor 2-like [Source:NCBI gene;Acc:103030591])

HSP 1 Score: 54.6842 bits (130), Expect = 3.180e-9
Identity = 31/102 (30.39%), Postives = 59/102 (57.84%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSS-FLIQSALILFAVFVNSTLVLGTLFIPKIYALF 306
            V + CN       + + +N + +++ T++ FKTRK PENF+++K I      +  +WL+  P    T+S + +Q+  +  +V ++ ++VLG LF PKI+ + 
Sbjct:  709 VTLKCNSLDSSMLVSLTYNCILIILCTFYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFQPIFYVTASDYRVQTTTMCISVSLSGSVVLGCLFAPKIHIIL 810          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004832.1 (pep scaffold:Pmarinus_7.0:GL494148:1537:3056:-1 gene:ENSPMAG00000004396.1 transcript:ENSPMAT00000004832.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.7658 bits (138), Expect = 1.143e-11
Identity = 36/108 (33.33%), Postives = 63/108 (58.33%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L ++  TY+ FKTR +PENF+++K I      +  +WL+ +P    +   ++ + L    V ++ST+VLG +F+PK+Y + + K E N
Sbjct:   14 VNLICNTSNLAMVAPLGFNGLLIMSCTYYAFKTRNVPENFNEAKYIAFTMYTTCIIWLAFVPIYFGSKYKIVTTCL---CVSLSSTVVLGCMFLPKVY-IILAKPERN 117          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003019.1 (pep scaffold:Pmarinus_7.0:GL478098:6745:34806:-1 gene:ENSPMAG00000002755.1 transcript:ENSPMAT00000003019.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 44.2838 bits (103), Expect = 2.375e-6
Identity = 31/100 (31.00%), Postives = 57/100 (57.00%), Query Frame = 1
Query:   49 VGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFL----IQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELNYNQ 336
            +G++ L ++  T +  KTR +PE F+++K I      +  +WL  IP    TS  +    IQ+  +  A+ +++++ LG LF PK+Y + +L+ ELN  +
Sbjct:  256 LGYSILLMVTCTVYAIKTRGVPEGFNEAKPIGFTMYTTCIIWLIFIPIFFGTSQSVNKLGIQTTTLTIAMSLSASVSLGMLFCPKVY-IVLLRPELNVQK 354          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Medaka
Match: grm5a (metabotropic glutamate receptor 5 [Source:NCBI gene;Acc:101173805])

HSP 1 Score: 54.299 bits (129), Expect = 4.544e-9
Identity = 33/108 (30.56%), Postives = 62/108 (57.41%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ L  +F+PK+Y + + K E N
Sbjct:  734 VHLICNLTTLGVVAPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALCCMFVPKVY-IMLAKPEKN 837          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Medaka
Match: grm1a (glutamate metabotropic receptor 1 [Source:NCBI gene;Acc:101165118])

HSP 1 Score: 54.299 bits (129), Expect = 4.902e-9
Identity = 34/108 (31.48%), Postives = 60/108 (55.56%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L ++  TY+ FKTR +P NF+++K I      +  +WL+ +P    +   +I ++   F+V ++ T+ L  +F PK+Y + I K E N
Sbjct:  748 VYLICNTSNVGMVAPLGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSDYKIITTS---FSVSLSVTVALSCMFTPKMY-IIIAKPERN 851          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Medaka
Match: grm2a (glutamate metabotropic receptor 2 [Source:NCBI gene;Acc:101169193])

HSP 1 Score: 53.5286 bits (127), Expect = 6.826e-9
Identity = 31/102 (30.39%), Postives = 58/102 (56.86%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSS-FLIQSALILFAVFVNSTLVLGTLFIPKIYALF 306
            V + CN       + + +N + +++ T + FKTRK PENF+++K I      +  +WL+  P    T+S + +Q+  +  +V ++ ++VLG LF PKI+ + 
Sbjct:  708 VTLKCNNRDSSMLLSLTYNCILIILCTVYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFQPIFYVTASDYRVQTTTMCISVSLSGSVVLGCLFAPKIHIIL 809          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Medaka
Match: grm5b (glutamate metabotropic receptor 5 [Source:NCBI gene;Acc:101164491])

HSP 1 Score: 53.5286 bits (127), Expect = 7.895e-9
Identity = 33/108 (30.56%), Postives = 62/108 (57.41%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ L  +F+PK+Y + + K E N
Sbjct:  746 VNLICNTTNLGVVAPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALCCMFVPKVY-IILAKPERN 849          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Medaka
Match: grm5b (glutamate metabotropic receptor 5 [Source:NCBI gene;Acc:101164491])

HSP 1 Score: 53.5286 bits (127), Expect = 8.751e-9
Identity = 33/108 (30.56%), Postives = 62/108 (57.41%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELN 327
            V ++CN        P+G+N L +L  T++ FKTR +P NF+++K I      +  +WL+ +P    ++  +I    + F+V +++T+ L  +F+PK+Y + + K E N
Sbjct:  724 VNLICNTTNLGVVAPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIIT---MCFSVSLSATVALCCMFVPKVY-IILAKPERN 827          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Planmine SMEST
Match: SMESG000064459.1 (SMESG000064459.1)

HSP 1 Score: 213.001 bits (541), Expect = 1.632e-65
Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 1
Query:    1 YVEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELNYNQTLHNTVEVSAR 369
            YVEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKCIFIFSSCSIFLWLSLIPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELNYNQTLHNTVEVSAR
Sbjct:  709 YVEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKCIFIFSSCSIFLWLSLIPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELNYNQTLHNTVEVSAR 831          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Planmine SMEST
Match: SMESG000064049.1 (SMESG000064049.1)

HSP 1 Score: 48.521 bits (114), Expect = 3.323e-7
Identity = 34/121 (28.10%), Postives = 66/121 (54.55%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELNYNQTLHNTVEVSA 366
            V ++CN +    +I +G++ + +   T++ FKTR LPENF+++K I      +  +W+ L+   L+T+   ++   + FA+   +T+ +  LF+PK+Y + + K E N       T E+  
Sbjct:  566 VRLICNTESQDLFISMGFDAVLVCFCTWYAFKTRNLPENFNEAKFIGFTMYTTCIIWIGLVVIYLSTN---LKEISLCFAMHFTATICIVFLFLPKLY-IILFKPERNERSAFTTTTEIRC 682          
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Planmine SMEST
Match: SMESG000064054.1 (SMESG000064054.1)

HSP 1 Score: 47.7506 bits (112), Expect = 4.861e-7
Identity = 34/122 (27.87%), Postives = 64/122 (52.46%), Query Frame = 1
Query:    4 VEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKXXXXXXXXXXXXXXXXXPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYALFILKEELNYNQTLHNTVEVSAR 369
            V ++CN       I +G++ + +   T++ FKTR LPENF+++K I      +  +W+ L+   L+T    +Q   + FA+ + +T+ +  LF+PK+Y + + + E N       T E+   
Sbjct:  232 VYLICNTPSSSLMISMGFDAVLIAFCTWYAFKTRNLPENFNEAKFIGFTMYTTCIIWIGLVVIYLSTD---LQVISLCFAIDLTATICIILLFLPKVY-IILFRPEKNERSAFTTTTEIQCH 349          
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GRM51.620e-931.48glutamate metabotropic receptor 5 [Source:HGNC Sym... [more]
GRM51.815e-931.48glutamate metabotropic receptor 5 [Source:HGNC Sym... [more]
GRM51.815e-931.48glutamate metabotropic receptor 5 [Source:HGNC Sym... [more]
GRM12.380e-933.33glutamate metabotropic receptor 1 [Source:HGNC Sym... [more]
GRM12.380e-933.33glutamate metabotropic receptor 1 [Source:HGNC Sym... [more]
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BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mgl-29.522e-829.13Metabotropic GLutamate receptor family [Source:Un... [more]
mgl-21.013e-729.13Metabotropic GLutamate receptor family [Source:Un... [more]
mgl-21.170e-729.13Metabotropic GLutamate receptor family [Source:Un... [more]
mgl-38.860e-731.96Metabotropic GLutamate receptor family [Source:Un... [more]
mgl-38.860e-731.96Metabotropic GLutamate receptor family [Source:Un... [more]
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BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
grm1a8.145e-1032.41glutamate receptor, metabotropic 1a [Source:ZFIN;A... [more]
grm1a8.149e-1032.41glutamate receptor, metabotropic 1a [Source:ZFIN;A... [more]
grm1b1.142e-933.33glutamate receptor, metabotropic 1b [Source:ZFIN;A... [more]
grm1b1.142e-933.33glutamate receptor, metabotropic 1b [Source:ZFIN;A... [more]
grm5b1.872e-929.63glutamate receptor, metabotropic 5b [Source:ZFIN;A... [more]
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BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GRM56.774e-1031.48glutamate receptor, metabotropic 5 [Source:NCBI ge... [more]
GRM16.901e-1033.33glutamate receptor, metabotropic 1 [Source:NCBI ge... [more]
GRM57.029e-1031.48glutamate receptor, metabotropic 5 [Source:NCBI ge... [more]
GRM57.097e-1031.48glutamate receptor, metabotropic 5 [Source:NCBI ge... [more]
GRM57.453e-1031.48glutamate receptor, metabotropic 5 [Source:NCBI ge... [more]
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BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Grm16.971e-1034.26glutamate receptor, metabotropic 1 [Source:MGI Sym... [more]
Grm16.971e-1034.26glutamate receptor, metabotropic 1 [Source:MGI Sym... [more]
Grm59.528e-1031.48glutamate receptor, metabotropic 5 [Source:MGI Sym... [more]
Grm51.068e-931.48glutamate receptor, metabotropic 5 [Source:MGI Sym... [more]
Grm51.068e-931.48glutamate receptor, metabotropic 5 [Source:MGI Sym... [more]
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BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P23385|GRM1_RAT4.318e-934.26Metabotropic glutamate receptor 1 OS=Rattus norveg... [more]
sp|P31424|GRM5_RAT6.665e-931.48Metabotropic glutamate receptor 5 OS=Rattus norveg... [more]
sp|Q3UVX5|GRM5_MOUSE6.665e-931.48Metabotropic glutamate receptor 5 OS=Mus musculus ... [more]
sp|P41594|GRM5_HUMAN7.780e-931.48Metabotropic glutamate receptor 5 OS=Homo sapiens ... [more]
sp|Q13255|GRM1_HUMAN1.143e-833.33Metabotropic glutamate receptor 1 OS=Homo sapiens ... [more]
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BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8JG121.760e-935.51G_PROTEIN_RECEP_F3_4 domain-containing protein OS=... [more]
A0A1I8HVG33.224e-934.58G_PROTEIN_RECEP_F3_4 domain-containing protein OS=... [more]
A0A1I8HDN73.243e-934.58G_PROTEIN_RECEP_F3_4 domain-containing protein OS=... [more]
A0A0L8FPT14.774e-933.33G_PROTEIN_RECEP_F3_4 domain-containing protein (Fr... [more]
W4YWK56.602e-932.82G_PROTEIN_RECEP_F3_4 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
grm1a4.737e-1032.41glutamate receptor, metabotropic 1a [Source:ZFIN;A... [more]
grm1b1.026e-933.33glutamate receptor, metabotropic 1b [Source:ZFIN;A... [more]
grm2a1.518e-930.39metabotropic glutamate receptor 2 [Source:NCBI gen... [more]
grm5b1.528e-929.63metabotropic glutamate receptor 5 [Source:NCBI gen... [more]
grm2b3.180e-930.39metabotropic glutamate receptor 2-like [Source:NCB... [more]
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BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000004832.11.143e-1133.33pep scaffold:Pmarinus_7.0:GL494148:1537:3056:-1 ge... [more]
ENSPMAT00000003019.12.375e-631.00pep scaffold:Pmarinus_7.0:GL478098:6745:34806:-1 g... [more]
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BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
grm5a4.544e-930.56metabotropic glutamate receptor 5 [Source:NCBI gen... [more]
grm1a4.902e-931.48glutamate metabotropic receptor 1 [Source:NCBI gen... [more]
grm2a6.826e-930.39glutamate metabotropic receptor 2 [Source:NCBI gen... [more]
grm5b7.895e-930.56glutamate metabotropic receptor 5 [Source:NCBI gen... [more]
grm5b8.751e-930.56glutamate metabotropic receptor 5 [Source:NCBI gen... [more]
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BLAST of G_PROTEIN_RECEP_F3_4 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000064459.11.632e-65100.00SMESG000064459.1[more]
SMESG000064049.13.323e-728.10SMESG000064049.1[more]
SMESG000064054.14.861e-727.87SMESG000064054.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033858 ID=SMED30033858|Name=G_PROTEIN_RECEP_F3_4 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=420bp
TACGTGGAAATGGTATGCAATCGAGACATTCCTCGATTCTGGATTCCGGT
CGGTTGGAACTGGCTGAAATTACTGATATCAACATATTGGGGTTTTAAAA
CTAGAAAACTACCAGAAAATTTTCATGACTCCAAATGTATTTTCATATTT
TCATCATGTTCAATATTTCTTTGGCTAAGTCTCATTCCATCGATATTGGC
GACCTCTTCATTTCTTATTCAATCAGCTCTGATTTTATTTGCCGTATTTG
TTAATTCGACACTAGTTTTAGGAACACTTTTTATTCCAAAGATTTATGCT
TTGTTTATTTTGAAAGAGGAACTCAATTACAATCAAACACTCCACAATAC
TGTTGAAGTTTCGGCCCGTTGAAAATTCTTTTCGTTTTCGGAAACTGTTT
TTGTGTGAAAATCAATCAAG
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protein sequence of SMED30033858-orf-1

>SMED30033858-orf-1 ID=SMED30033858-orf-1|Name=SMED30033858-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=124bp
YVEMVCNRDIPRFWIPVGWNWLKLLISTYWGFKTRKLPENFHDSKCIFIF
SSCSIFLWLSLIPSILATSSFLIQSALILFAVFVNSTLVLGTLFIPKIYA
LFILKEELNYNQTLHNTVEVSAR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000337GPCR_3
IPR017978GPCR_3_C
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000337GPCR, family 3PRINTSPR00248GPCRMGRcoord: 19..42
score: 37.89
coord: 42..63
score: 24.43
IPR017978GPCR family 3, C-terminalPFAMPF000037tm_3coord: 5..99
e-value: 3.6E-17
score: 62.9
IPR017978GPCR family 3, C-terminalPROSITEPS50259G_PROTEIN_RECEP_F3_4coord: 1..117
score: 15.972
NoneNo IPR availablePANTHERPTHR24060METABOTROPIC GLUTAMATE RECEPTORcoord: 2..109
NoneNo IPR availablePANTHERPTHR24060:SF30METABOTROPIC GLUTAMATE RECEPTOR 5coord: 2..109
NoneNo IPR availableCDDcd139537tm_classC_mGluR-likecoord: 1..102
e-value: 7.30178E-19
score: 76.8917
NoneNo IPR availableTMHMMTMhelixcoord: 76..95
NoneNo IPR availableTMHMMTMhelixcoord: 47..69
NoneNo IPR availableTMHMMTMhelixcoord: 13..32