Transcription factor AP-4

Overview
NameTranscription factor AP-4
Smed IDSMED30033844
Length (bp)2109
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Transcription factor AP-4 (SMED30033844) t-SNE clustered cells

Violin plots show distribution of expression levels for Transcription factor AP-4 (SMED30033844) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Transcription factor AP-4 (SMED30033844) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Transcription factor AP-4 (SMED30033844) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033844

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
central nervous systemSMED30033844h1SMcG0021916 dd_Smed_v4_8205_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
X2 cellSMED30033844h1SMcG0021916 SmedASXL_006645SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30033844h1SMcG0021916 SmedASXL_011636SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30033844h1SMcG0021916 HO007476smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30033844h1SMcG0021916 HO007476smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
central nervous systemSMED30033844h1SMcG0021916 HO007476smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
progenitor cellSMED30033844h1SMcG0021916 HO007476smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
stem cellSMED30033844h1SMcG0021916 HO007476smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30033844h1SMcG0021916 HO007476smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Human
Match: TFAP4 (transcription factor AP-4 [Source:HGNC Symbol;Acc:HGNC:11745])

HSP 1 Score: 80.1073 bits (196), Expect = 1.640e-15
Identity = 39/56 (69.64%), Postives = 50/56 (89.29%), Query Frame = 2 
Query:  233 DKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +++++REIANSNERRRMQSIN GF++LK L+P T +GEKLSKA+ILQ TA+YI SL
Sbjct:   45 ERRIRREIANSNERRRMQSINAGFQSLKTLIPHT-DGEKLSKAAILQQTAEYIFSL 99
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Human
Match: TFAP4 (transcription factor AP-4 [Source:HGNC Symbol;Acc:HGNC:11745])

HSP 1 Score: 54.299 bits (129), Expect = 2.247e-8
Identity = 28/40 (70.00%), Postives = 34/40 (85.00%), Query Frame = 2 
Query:  281 MQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            MQSIN GF++LK L+P T +GEKLSKA+ILQ TA+YI SL
Sbjct:    1 MQSINAGFQSLKTLIPHT-DGEKLSKAAILQQTAEYIFSL 39
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Human
Match: FIGLA (folliculogenesis specific bHLH transcription factor [Source:HGNC Symbol;Acc:HGNC:24669])

HSP 1 Score: 48.9062 bits (115), Expect = 9.590e-6
Identity = 28/80 (35.00%), Postives = 44/80 (55.00%), Query Frame = 2 
Query:  182 VCKVRR-------NDHNYRQVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            VC+++R       +  N + V E     +R +AN+ ER R++++N GF  LK L+P   +  K SK  IL+   +YIQ L
Sbjct:   43 VCRLKRLPSGGYSSTENLQLVLE-----RRRVANAKERERIKNLNRGFARLKALVPFLPQSRKPSKVDILKGATEYIQVL 117
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Celegans
Match: hlh-11 (Helix-loop-helix protein 11 [Source:UniProtKB/Swiss-Prot;Acc:P34474])

HSP 1 Score: 74.7146 bits (182), Expect = 7.586e-14
Identity = 36/60 (60.00%), Postives = 45/60 (75.00%), Query Frame = 2 
Query:  224 YEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLI 403
             + D++M+R+IAN NERRRMQSIN GF  L+ LLP   EGEKLSKA+ILQ TA  +  L+
Sbjct:  104 LDPDRRMRRQIANCNERRRMQSINAGFLALRALLPRK-EGEKLSKAAILQQTADMVHQLL 162
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Celegans
Match: hlh-11 (Helix-loop-helix protein 11 [Source:UniProtKB/Swiss-Prot;Acc:P34474])

HSP 1 Score: 74.7146 bits (182), Expect = 7.586e-14
Identity = 36/60 (60.00%), Postives = 45/60 (75.00%), Query Frame = 2 
Query:  224 YEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLI 403
             + D++M+R+IAN NERRRMQSIN GF  L+ LLP   EGEKLSKA+ILQ TA  +  L+
Sbjct:  104 LDPDRRMRRQIANCNERRRMQSINAGFLALRALLPRK-EGEKLSKAAILQQTADMVHQLL 162
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Celegans
Match: hlh-11 (Helix-loop-helix protein 11 [Source:UniProtKB/Swiss-Prot;Acc:P34474])

HSP 1 Score: 74.3294 bits (181), Expect = 7.691e-14
Identity = 36/60 (60.00%), Postives = 45/60 (75.00%), Query Frame = 2 
Query:  224 YEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLI 403
             + D++M+R+IAN NERRRMQSIN GF  L+ LLP   EGEKLSKA+ILQ TA  +  L+
Sbjct:  104 LDPDRRMRRQIANCNERRRMQSINAGFLALRALLPRK-EGEKLSKAAILQQTADMVHQLL 162
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Celegans
Match: hlh-11 (Helix-loop-helix protein 11 [Source:UniProtKB/Swiss-Prot;Acc:P34474])

HSP 1 Score: 74.3294 bits (181), Expect = 7.691e-14
Identity = 36/60 (60.00%), Postives = 45/60 (75.00%), Query Frame = 2 
Query:  224 YEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLI 403
             + D++M+R+IAN NERRRMQSIN GF  L+ LLP   EGEKLSKA+ILQ TA  +  L+
Sbjct:  104 LDPDRRMRRQIANCNERRRMQSINAGFLALRALLPRK-EGEKLSKAAILQQTADMVHQLL 162
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Celegans
Match: hlh-11 (Helix-loop-helix protein 11 [Source:UniProtKB/Swiss-Prot;Acc:P34474])

HSP 1 Score: 70.8626 bits (172), Expect = 1.126e-12
Identity = 35/55 (63.64%), Postives = 42/55 (76.36%), Query Frame = 2 
Query:  239 KMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLI 403
            +M+R+IAN NERRRMQSIN GF  L+ LLP   EGEKLSKA+ILQ TA  +  L+
Sbjct:  111 RMRRQIANCNERRRMQSINAGFLALRALLPRK-EGEKLSKAAILQQTADMVHQLL 164
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Fly
Match: crp (gene:FBgn0001994 transcript:FBtr0343814)

HSP 1 Score: 80.4925 bits (197), Expect = 2.219e-15
Identity = 40/67 (59.70%), Postives = 52/67 (77.61%), Query Frame = 2 
Query:  200 NDHNYRQVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
             D + + + + +K+M+REIANSNERRRMQSIN GF++L+ LLP   EGEKLSKA+ILQ T +YI  L
Sbjct:   82 GDGSGKPLVDSEKRMRREIANSNERRRMQSINAGFQSLRSLLPR-HEGEKLSKAAILQQTFQYIVEL 147
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Fly
Match: crp (gene:FBgn0001994 transcript:FBtr0080854)

HSP 1 Score: 80.4925 bits (197), Expect = 2.219e-15
Identity = 40/67 (59.70%), Postives = 52/67 (77.61%), Query Frame = 2 
Query:  200 NDHNYRQVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
             D + + + + +K+M+REIANSNERRRMQSIN GF++L+ LLP   EGEKLSKA+ILQ T +YI  L
Sbjct:   82 GDGSGKPLVDSEKRMRREIANSNERRRMQSINAGFQSLRSLLPR-HEGEKLSKAAILQQTFQYIVEL 147
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Fly
Match: ato (gene:FBgn0010433 transcript:FBtr0081828)

HSP 1 Score: 50.0618 bits (118), Expect = 3.946e-6
Identity = 25/62 (40.32%), Postives = 36/62 (58.06%), Query Frame = 2 
Query:  215 RQVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +Q+    K+ +R  AN+ ERRRMQ++N  F+ L+Q LP      +LSK   LQ    YI +L
Sbjct:  246 KQITPVVKRKRRLAANARERRRMQNLNQAFDRLRQYLPCLGNDRQLSKHETLQMAQTYISAL 307
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Fly
Match: cato (gene:FBgn0024249 transcript:FBtr0087183)

HSP 1 Score: 48.1358 bits (113), Expect = 6.598e-6
Identity = 29/86 (33.72%), Postives = 45/86 (52.33%), Query Frame = 2 
Query:  236 KKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNML-NSKEKFPLSKS 490
            +K +R+ AN+ ER+RM  +N  FE L++++P     +KLSK   LQ    YI +L   L + + EV      +  +S   F LS  
Sbjct:  101 QKRRRQAANARERKRMNGLNAAFERLREVVPAPSIDQKLSKFETLQMAQSYILALCDLLNNGDVEVDAAAYTIFGDSDSGFGLSGG 186
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Fly
Match: tx (gene:FBgn0263118 transcript:FBtr0085009)

HSP 1 Score: 49.2914 bits (116), Expect = 7.695e-6
Identity = 29/73 (39.73%), Postives = 41/73 (56.16%), Query Frame = 2 
Query:  239 KMKREIANSNERRRMQSINMGFETLKQLLPMTMEG-------EKLSKASILQFTAKYIQSLI--VRLTSLEKE 430
            K +R+ AN+ ER RM+ IN  FETL+  +P  ++G       EKL+K + L+   KYI  L   +R  S E E
Sbjct:   93 KYRRKTANARERTRMREINTAFETLRHCVPEAIKGEDAANTNEKLTKITTLRLAMKYITMLTDSIRDPSYESE 165
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Zebrafish
Match: tfap4 (transcription factor AP-4 (activating enhancer binding protein 4) [Source:ZFIN;Acc:ZDB-GENE-090810-1])

HSP 1 Score: 78.5666 bits (192), Expect = 3.547e-15
Identity = 38/56 (67.86%), Postives = 50/56 (89.29%), Query Frame = 2 
Query:  233 DKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +++++REIANSNERRRMQSIN GF++LK L+P + +GEKLSKA+ILQ TA+YI SL
Sbjct:   45 ERRIRREIANSNERRRMQSINAGFQSLKTLIPHS-DGEKLSKAAILQQTAEYIFSL 99
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Zebrafish
Match: atoh7 (atonal bHLH transcription factor 7 [Source:NCBI gene;Acc:58216])

HSP 1 Score: 47.3654 bits (111), Expect = 5.555e-6
Identity = 22/59 (37.29%), Postives = 38/59 (64.41%), Query Frame = 2 
Query:  227 EYDKKMKREIA-NSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +++  M+R +A N+ ER+RMQ +N  F+ L++++P   + +KLSK   LQ    YI +L
Sbjct:   22 KFESAMRRRMAANARERKRMQGLNTAFDRLRKVVPQWGQDKKLSKYETLQMALSYIMAL 80
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Zebrafish
Match: twist3 (twist3 [Source:ZFIN;Acc:ZDB-GENE-000210-7])

HSP 1 Score: 48.1358 bits (113), Expect = 8.515e-6
Identity = 31/80 (38.75%), Postives = 46/80 (57.50%), Query Frame = 2 
Query:  218 QVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNML 457
            Q +E D   +R IAN  ER+R QS+N  F +L++++P T+  +KLSK  IL+  ++YI  L   L S E +      N L
Sbjct:   98 QPFE-DLHTQRVIANVRERQRTQSLNDAFASLRKIIP-TLPSDKLSKIQILKLASRYIDFLYQVLQSDEMDAKLASCNYL 175
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Xenopus
Match: rbm28 (RNA binding motif protein 28 [Source:Xenbase;Acc:XB-GENE-919947])

HSP 1 Score: 80.1073 bits (196), Expect = 1.283e-15
Identity = 39/56 (69.64%), Postives = 50/56 (89.29%), Query Frame = 2 
Query:  233 DKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +++++REIANSNERRRMQSIN GF++LK L+P T +GEKLSKA+ILQ TA+YI SL
Sbjct:   45 ERRIRREIANSNERRRMQSINAGFQSLKTLIPHT-DGEKLSKAAILQQTAEYIFSL 99
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Xenopus
Match: ppil6 (peptidylprolyl isomerase like 6 [Source:Xenbase;Acc:XB-GENE-979458])

HSP 1 Score: 51.2174 bits (121), Expect = 3.802e-7
Identity = 23/52 (44.23%), Postives = 34/52 (65.38%), Query Frame = 2 
Query:  245 KREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +R  AN+ ERRRMQ +N  F++L++++P   E +KLSK   LQ    YI +L
Sbjct:   35 RRMAANARERRRMQGLNTAFDSLRKVVPQWGEDKKLSKYETLQMALSYIMAL 86
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Xenopus
Match: st6gal1 (ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 [Source:Xenbase;Acc:XB-GENE-983202])

HSP 1 Score: 47.3654 bits (111), Expect = 6.022e-6
Identity = 30/87 (34.48%), Postives = 43/87 (49.43%), Query Frame = 2 
Query:  158 FTDCEEKYVCKVRRNDHN---YRQ---VYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
             T C   ++    R  H+   Y Q        KK  R  AN+ ER+RM S+N+ F+ L+ ++P T   +KLSK+  LQ    YI  L
Sbjct:    6 VTPCRISHLSLSHRKSHDIIGYSQNICALRQSKKSCRLAANARERKRMLSLNLAFDRLRSVIPTTQNKKKLSKSETLQMAKIYILML 92
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Mouse
Match: Tfap4 (transcription factor AP4 [Source:MGI Symbol;Acc:MGI:103239])

HSP 1 Score: 79.337 bits (194), Expect = 2.848e-16
Identity = 39/56 (69.64%), Postives = 50/56 (89.29%), Query Frame = 2 
Query:  233 DKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +++++REIANSNERRRMQSIN GF++LK L+P T +GEKLSKA+ILQ TA+YI SL
Sbjct:   25 ERRIRREIANSNERRRMQSINAGFQSLKTLIPHT-DGEKLSKAAILQQTAEYIFSL 79
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Mouse
Match: Tfap4 (transcription factor AP4 [Source:MGI Symbol;Acc:MGI:103239])

HSP 1 Score: 80.1073 bits (196), Expect = 9.459e-16
Identity = 39/56 (69.64%), Postives = 50/56 (89.29%), Query Frame = 2 
Query:  233 DKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +++++REIANSNERRRMQSIN GF++LK L+P T +GEKLSKA+ILQ TA+YI SL
Sbjct:   45 ERRIRREIANSNERRRMQSINAGFQSLKTLIPHT-DGEKLSKAAILQQTAEYIFSL 99
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Mouse
Match: Tfap4 (transcription factor AP4 [Source:MGI Symbol;Acc:MGI:103239])

HSP 1 Score: 54.6842 bits (130), Expect = 6.289e-8
Identity = 28/40 (70.00%), Postives = 34/40 (85.00%), Query Frame = 2 
Query:  281 MQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            MQSIN GF++LK L+P T +GEKLSKA+ILQ TA+YI SL
Sbjct:    1 MQSINAGFQSLKTLIPHT-DGEKLSKAAILQQTAEYIFSL 39
[Close]
BLAST of Transcription factor AP-4 vs. UniProt/SwissProt
Match: sp|Q01664|TFAP4_HUMAN (Transcription factor AP-4 OS=Homo sapiens OX=9606 GN=TFAP4 PE=1 SV=2)

HSP 1 Score: 80.1073 bits (196), Expect = 7.876e-15
Identity = 39/56 (69.64%), Postives = 50/56 (89.29%), Query Frame = 2 
Query:  233 DKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +++++REIANSNERRRMQSIN GF++LK L+P T +GEKLSKA+ILQ TA+YI SL
Sbjct:   45 ERRIRREIANSNERRRMQSINAGFQSLKTLIPHT-DGEKLSKAAILQQTAEYIFSL 99
[Close]
BLAST of Transcription factor AP-4 vs. UniProt/SwissProt
Match: sp|P34474|HLH11_CAEEL (Helix-loop-helix protein 11 OS=Caenorhabditis elegans OX=6239 GN=hlh-11 PE=3 SV=3)

HSP 1 Score: 70.8626 bits (172), Expect = 1.433e-11
Identity = 35/55 (63.64%), Postives = 42/55 (76.36%), Query Frame = 2 
Query:  239 KMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLI 403
            +M+R+IAN NERRRMQSIN GF  L+ LLP   EGEKLSKA+ILQ TA  +  L+
Sbjct:  111 RMRRQIANCNERRRMQSINAGFLALRALLPRK-EGEKLSKAAILQQTADMVHQLL 164
[Close]
BLAST of Transcription factor AP-4 vs. UniProt/SwissProt
Match: sp|O13126|ATO7B_XENLA (Protein atonal homolog 7-B OS=Xenopus laevis OX=8355 GN=atoh7-b PE=1 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 5.918e-7
Identity = 26/61 (42.62%), Postives = 38/61 (62.30%), Query Frame = 2 
Query:  245 KREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEK 427
            +R  AN+ ERRRMQ +N  F++L++++P   E +KLSK   LQ    YI +L   LT  E+
Sbjct:   34 RRLAANARERRRMQGLNTAFDSLRKVVPQWGEDKKLSKYETLQMALSYIMALSRILTEAER 94
[Close]
BLAST of Transcription factor AP-4 vs. UniProt/SwissProt
Match: sp|Q10574|LIN32_CAEEL (Protein lin-32 OS=Caenorhabditis elegans OX=6239 GN=lin-32 PE=1 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 6.690e-7
Identity = 24/54 (44.44%), Postives = 36/54 (66.67%), Query Frame = 2 
Query:  239 KMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +M+R  AN  ERRRM ++N+ ++ L+++LP    G+KLSK   LQ   KYI+ L
Sbjct:   71 RMRRSAANERERRRMNTLNVAYDELREVLPEIDSGKKLSKFETLQMAQKYIECL 124
[Close]
BLAST of Transcription factor AP-4 vs. UniProt/SwissProt
Match: sp|O13125|ATO7A_XENLA (Protein atonal homolog 7-A OS=Xenopus laevis OX=8355 GN=atoh7-a PE=1 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 5.579e-6
Identity = 24/61 (39.34%), Postives = 38/61 (62.30%), Query Frame = 2 
Query:  245 KREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEK 427
            +R  AN+ ERRRMQ +N  F++L++++P   E ++LSK   LQ    YI +L   L+  E+
Sbjct:   34 RRLAANARERRRMQGLNTAFDSLRKVVPQWGEDKQLSKYETLQMALSYIMALSRILSEAER 94
[Close]
BLAST of Transcription factor AP-4 vs. TrEMBL
Match: A0A5J4P0Q1 (Transcription factor AP-4 OS=Paragonimus westermani OX=34504 GN=DEA37_0011392 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 2.356e-26
Identity = 87/229 (37.99%), Postives = 123/229 (53.71%), Query Frame = 2 
Query:  140 MEKRHAFTDCEEKYVCKVRRNDHNYRQV----YEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNM----LNSKEKFPLSKSPSLMQE-----------QNRKRRLNS---CSVDLEIAQIENQDSTLDFXXXXXXXXXXXXXQENSNSITCQASEKTPGSLEHLVAAIEQVEGEKFYVGSINNSE 760
            MEKR +  D       K RR      Q      E +K+++REIANSNERRRMQSIN GFE+L+ LLP   +GEK+SKA+ILQ TA+YI SL+ R++ LE EVA Y+++          +FP+    S  ++           ++R+RR++    C  DL  + I       D  ++  SN  +S    +  S     +  T   LEHLV AIEQ+EG +   GS N+S+
Sbjct:    1 MEKRRSHADFTGDGCDKSRRPSDGSLQPGQEHLEQEKRVRREIANSNERRRMQSINAGFESLRILLPPVQDGEKMSKATILQLTAEYINSLVNRVSLLENEVAIYRQSTGIQPTTILPEFPVDFGLSRRRKSGEHKKRTGFNRSRRRRVDPERQC--DLSRSDIARSSPATDIHHTSPSNSFTSSISVSPPSPIPDGTSSTSARLEHLVMAIEQIEGGRAIYGSGNDSQ 227
[Close]
BLAST of Transcription factor AP-4 vs. TrEMBL
Match: A0A0X3Q3I6 (Transcription factor AP-4 OS=Schistocephalus solidus OX=70667 GN=TFAP4 PE=4 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 2.362e-26
Identity = 83/228 (36.40%), Postives = 123/228 (53.95%), Query Frame = 2 
Query:  119 MSSNQDIMEKRHAFTDCEEKYVCKVRRNDHNYRQV------YEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNMLNSKEKFPLSKSPSLMQEQNRKRRL-NSCSVDLEI-AQIENQDSTLDFXXXXXXXXXXXXXQENSNSITC------------------QASEKTPGSLEHLVAAIEQVEG 724
            MS   +++++R  +T+  E  + K RR+  +Y          E +K+++REIAN NERRRMQSIN GFE L+ LLP   EGEKLSKA+ILQ TAK+I SL++R+T+LE ++A Y++  L S        +  +     RKRR  +  + DL +     N D+  +F++  + N     S+   N   C                  +++  T   LEHLV AIEQ+EG
Sbjct:    1 MSQKAEVIDRRRYYTETREDILSKPRRSCSDYGSAKVGHLQVENEKRVRREIANCNERRRMQSINAGFENLRVLLPPVQEGEKLSKATILQLTAKFINSLLMRVTALEHQIAVYRQAGLPSAAVL-ADITDEVALGCGRKRRADDDKNFDLGVKVGRRNTDNDPNFTDLDDGNTSGRSSECYDNHFVCSPTSGAQRISTMSSVSPTESTSSTAARLEHLVMAIEQIEG 227
[Close]
BLAST of Transcription factor AP-4 vs. TrEMBL
Match: A0A183QI35 (BHLH domain-containing protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 7.593e-24
Identity = 81/203 (39.90%), Postives = 117/203 (57.64%), Query Frame = 2 
Query:  224 YEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNM---LNSKEKFPLSKSPSLMQEQNRKRRLNSCS------------VDLEIAQIENQDSTLDFXXXXXXXXXXXXXQE-NSNSITCQASEK------TPGSLEHLVAAIEQVEGEKFYVGSINNSEIP 766
            ++ +K+++REIANSNERRRMQSIN GF++L+ LLP   +GEK+SKA+ILQ TAKYI +L++R++ LE EVA Y+++    LN     P+S   S     +RKR+ +               V++E     ++ S + FS      + SS     NS++  C AS +      T   LEHLV AIEQ+EG     G++N S  P
Sbjct:   32 HDQEKRVRREIANSNERRRMQSINAGFDSLRILLPSISDGEKMSKATILQLTAKYINTLLMRVSLLESEVAVYRQSAGLQLN-----PVSSELSGSSVTSRKRKSDDLKDRPDCIRNRRRRVEIEGIYDSSRTSYVHFSPESTDTHQSSPLTSFNSSTSICSASPQPDSTSSTAARLEHLVMAIEQIEGG----GALNVSPQP 225
[Close]
BLAST of Transcription factor AP-4 vs. TrEMBL
Match: A0A0R3WDZ8 (BHLH domain-containing protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS9017 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 8.555e-24
Identity = 83/227 (36.56%), Postives = 124/227 (54.63%), Query Frame = 2 
Query:  119 MSSNQDIMEKRHAFTDCEEKYVCKVRRN---DHNYRQVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNMLNSKEKFPLSKSPSLMQEQ-----NRKRRLNSCSVDLEIAQIENQD---STLDFXXXXXXXXXXXXXQEN--------------SNSITCQASEKTPGSLEHLVAAIEQVEG 724
            MS   +++++R  F++  E+   K RR+   D +     E +K+++REIAN NERRRMQSIN GFE L+ LLP T +GEKLSKASILQ TAK + +L++++ +LE+E+A Y+++        P S   + + E+      RKRR       LE  ++  QD   + LD  +   S   S     N              S++   +++  T   LEHLV AIEQ+EG
Sbjct:    1 MSHKVELIDRRRTFSEYSEESGMKHRRSNISDISKSGQVENEKRVRREIANCNERRRMQSINAGFENLRILLPPTQDGEKLSKASILQQTAKLVNTLLLKVNALEQELAIYRRST-----GLPPSSVLADITEEVATGFARKRRTEDQGKTLESLKMAKQDAETALLDLDDGNASGRSSDCYDGNFGCSPSSATRLPPVSSASPSESTVGTAARLEHLVMAIEQIEG 222
[Close]
BLAST of Transcription factor AP-4 vs. TrEMBL
Match: G4LWQ5 (Activator protein 4 (Ap4), putative OS=Schistosoma mansoni OX=6183 GN=Smp_103540.2 PE=4 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 9.170e-24
Identity = 81/203 (39.90%), Postives = 117/203 (57.64%), Query Frame = 2 
Query:  224 YEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNM---LNSKEKFPLSKSPSLMQEQNRKRRLNSCS------------VDLEIAQIENQDSTLDFXXXXXXXXXXXXXQE-NSNSITCQASEK------TPGSLEHLVAAIEQVEGEKFYVGSINNSEIP 766
            ++ +K+++REIANSNERRRMQSIN GF++L+ LLP   +GEK+SKA+ILQ TAKYI +L++R++ LE EVA Y+++    LN     P+S   S     +RKRR +               V++E     ++ + + FS      + SS     NS++  C AS +      T   LEHLV AIEQ+EG     G++N S  P
Sbjct:   33 HDQEKRVRREIANSNERRRMQSINAGFDSLRILLPSISDGEKMSKATILQLTAKYINTLLMRVSLLESEVAVYRQSAGLQLN-----PVSSELSGSSVTSRKRRSDDLKDRPDCIRNRRRRVEIEGIYDSSRTNYVHFSPESTDTHQSSPLTSFNSSTSICSASPQPDSTSSTAARLEHLVMAIEQIEGG----GALNVSPQP 226
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Cavefish
Match: tfap4 (transcription factor AP-4 [Source:NCBI gene;Acc:103031420])

HSP 1 Score: 78.9518 bits (193), Expect = 2.065e-15
Identity = 38/56 (67.86%), Postives = 50/56 (89.29%), Query Frame = 2 
Query:  233 DKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +++++REIANSNERRRMQSIN GF++LK L+P + +GEKLSKA+ILQ TA+YI SL
Sbjct:   45 ERRIRREIANSNERRRMQSINAGFQSLKTLIPHS-DGEKLSKAAILQQTAEYIFSL 99
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Cavefish
Match: twist3 (twist3 [Source:ZFIN;Acc:ZDB-GENE-000210-7])

HSP 1 Score: 48.9062 bits (115), Expect = 2.209e-6
Identity = 31/80 (38.75%), Postives = 46/80 (57.50%), Query Frame = 2 
Query:  218 QVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNML 457
            Q +E D   +R IAN  ER+R QS+N  F +L++++P T+  +KLSK  IL+  ++YI  L   L S E +      N L
Sbjct:   58 QAFE-DVHSQRVIANVRERQRTQSLNDAFASLRKIIP-TLPSDKLSKIQILKLASRYIDFLYQVLQSDEMDAKLASCNYL 135
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004072.1 (pep scaffold:Pmarinus_7.0:GL492732:1750:4325:-1 gene:ENSPMAG00000003727.1 transcript:ENSPMAT00000004072.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.521 bits (114), Expect = 2.217e-6
Identity = 29/80 (36.25%), Postives = 40/80 (50.00%), Query Frame = 2 
Query:  233 DKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNMLNSKEK 472
            D    R+ AN  ERRRMQSIN  FE L+  +P     ++LSK   L+    YI S +  L   E+ +       L S++K
Sbjct:  128 DMSHLRQAANQRERRRMQSINAAFEGLRAHIPTLPYEKRLSKVDTLRLAIGYI-SFLGELVHSERPLRAQAGQPLLSQQK 206
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Sea Lamprey
Match: TWIST1 (twist family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:12428])

HSP 1 Score: 45.4394 bits (106), Expect = 2.380e-6
Identity = 25/61 (40.98%), Postives = 40/61 (65.57%), Query Frame = 2 
Query:  218 QVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            Q YE D + +R +AN  ER+R QS+N  F +L++++P T+  +KLSK   L+  ++YI  L
Sbjct:    1 QSYE-DLQNQRVLANVRERQRTQSLNEAFASLRKIIP-TLPSDKLSKIQTLKLASRYIDFL 59
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002470.1 (pep scaffold:Pmarinus_7.0:GL487131:18401:21594:-1 gene:ENSPMAG00000002259.1 transcript:ENSPMAT00000002470.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.4394 bits (106), Expect = 2.785e-6
Identity = 25/61 (40.98%), Postives = 34/61 (55.74%), Query Frame = 2 
Query:  230 YDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRL 412
            Y K ++R   NS ER R Q++N  F  L++LLP      KLSK  IL+   +YI  L+  L
Sbjct:   18 YLKLVRRIFTNSRERWRQQNVNGAFSELRRLLPTHPPDRKLSKNEILRLAMRYIDFLVALL 78
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Nematostella
Match: EDO39015 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBF3])

HSP 1 Score: 77.0258 bits (188), Expect = 7.713e-17
Identity = 38/56 (67.86%), Postives = 49/56 (87.50%), Query Frame = 2 
Query:  233 DKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +K+++REIANSNERRRMQSIN GF+ L+ L+P T EGEKLSKA+ILQ T++YI +L
Sbjct:   10 EKRLRREIANSNERRRMQSINSGFQALRMLIPNT-EGEKLSKAAILQQTSEYIFTL 64
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Nematostella
Match: EDO46193 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQN3])

HSP 1 Score: 50.0618 bits (118), Expect = 3.864e-8
Identity = 25/53 (47.17%), Postives = 32/53 (60.38%), Query Frame = 2 
Query:  242 MKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            MKR   NS ER R Q +N+ F  L++L+P      KLSK  IL+F  KYI+ L
Sbjct:    1 MKRLYTNSRERWRQQHVNLAFAELRKLIPTYPPERKLSKNEILRFAMKYIKFL 53
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Nematostella
Match: EDO36266 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ30])

HSP 1 Score: 45.4394 bits (106), Expect = 3.296e-6
Identity = 20/54 (37.04%), Postives = 36/54 (66.67%), Query Frame = 2 
Query:  239 KMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            + +R IAN+ ER R+ +++  FE+L++ +P   + +KLSK +IL+    YI +L
Sbjct:    1 RSRRLIANARERSRIHTMSEAFESLRKAVPSYSQDQKLSKLAILRLATSYISAL 54
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Nematostella
Match: EDO46194 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQN4])

HSP 1 Score: 48.9062 bits (115), Expect = 3.831e-6
Identity = 23/51 (45.10%), Postives = 31/51 (60.78%), Query Frame = 2 
Query:  248 REIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            R + N  ER R QS+N+ F  +++LLP     +KLSK  IL+   KYIQ L
Sbjct:   52 RTVTNKKERWRQQSVNLAFAEIRKLLPTYPPDKKLSKVEILRTAVKYIQFL 102
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Nematostella
Match: NVATO2 (Atonal-related protein 2; Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ22])

HSP 1 Score: 47.3654 bits (111), Expect = 6.762e-6
Identity = 21/55 (38.18%), Postives = 36/55 (65.45%), Query Frame = 2 
Query:  236 KKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            K+++R  AN  ERRRM+S+N   ++LK+ +P+     +++K  IL+    YI+SL
Sbjct:  139 KRIRRLRANDRERRRMKSLNRALDSLKKCIPVPQSKRRVTKLEILRIACNYIKSL 193
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Medaka
Match: TFAP4 (transcription factor AP-4 [Source:NCBI gene;Acc:101157191])

HSP 1 Score: 79.337 bits (194), Expect = 1.221e-15
Identity = 39/56 (69.64%), Postives = 49/56 (87.50%), Query Frame = 2 
Query:  233 DKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +++++REIANSNERRRMQSIN GF++LK LLP T +GEKLSKA+ILQ TA YI +L
Sbjct:   45 ERRIRREIANSNERRRMQSINAGFQSLKTLLPHT-DGEKLSKAAILQQTADYIFTL 99
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Medaka
Match: tfap4 (transcription factor AP-4 [Source:NCBI gene;Acc:101175405])

HSP 1 Score: 77.7962 bits (190), Expect = 4.276e-15
Identity = 37/56 (66.07%), Postives = 50/56 (89.29%), Query Frame = 2 
Query:  233 DKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            +++++REIANSNERRRMQSIN GF++LK L+P + +GEKLSKA+ILQ TA+YI +L
Sbjct:   45 ERRIRREIANSNERRRMQSINAGFQSLKTLIPHS-DGEKLSKAAILQQTAEYIFTL 99
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Medaka
Match: tfap4 (transcription factor AP-4 [Source:NCBI gene;Acc:101175405])

HSP 1 Score: 63.1586 bits (152), Expect = 2.452e-10
Identity = 30/47 (63.83%), Postives = 40/47 (85.11%), Query Frame = 2 
Query:  233 DKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQ 373
            +++++REIANSNERRRMQSIN GF++LK L+P + +GEKLSK S  Q
Sbjct:   45 ERRIRREIANSNERRRMQSINAGFQSLKTLIPHS-DGEKLSKVSCEQ 90
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Medaka
Match: atoh7 (atonal bHLH transcription factor 7 [Source:NCBI gene;Acc:101155052])

HSP 1 Score: 50.447 bits (119), Expect = 5.009e-7
Identity = 24/70 (34.29%), Postives = 41/70 (58.57%), Query Frame = 2 
Query:  245 KREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNM 454
            +R  AN+ ER+RMQ +N  F+ L++++P   + +KLSK   LQ    YI +L   LT   +  A +++ +
Sbjct:   32 RRMAANARERKRMQGLNTAFDRLRKVVPQWGQDKKLSKYETLQMALSYIMALSRILTDPRRHAAPHRQWL 101
[Close]
BLAST of Transcription factor AP-4 vs. Planmine SMEST
Match: SMESG000081049.1 (SMESG000081049.1)

HSP 1 Score: 666.766 bits (1719), Expect = 0.000e+0
Identity = 393/393 (100.00%), Postives = 393/393 (100.00%), Query Frame = 2 
Query:  119 MSSNQDIMEKRHAFTDCEEKYVCKVRRNDHNYRQVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNMLNSKEKFPLSKSPSLMQEQNRKRRLNSCSVDLEIAQIENQDSTLDFXXXXXXXXXXXXXQENSNSITCQASEKTPGSLEHLVAAIEQVEGEKFYVGSINNSEIPLQIMKKEINSAGSSVIELNDDSFENVDKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDTDNTPLLNSNADIKKQXXXXXXXXXXXVMKPTISPYTEKIILAVSQHPHMFENRASNYSFNISTQPTYQIISSDNLIDSTGLNRHFSGSQRMSIPHKYLHKPDIALSKNHTL 1297
            MSSNQDIMEKRHAFTDCEEKYVCKVRRNDHNYRQVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNMLNSKEKFPLSKSPSLMQEQNRKRRLNSCSVDLEIAQIENQDSTLDFSNSKNSNYLSSLSQENSNSITCQASEKTPGSLEHLVAAIEQVEGEKFYVGSINNSEIPLQIMKKEINSAGSSVIELNDDSFENVDKHHHHHHHHHSNNPPPTPPSHPLHHTQQQMQSIQSISDTDNTPLLNSNADIKKQSNSNNNNNNNSVMKPTISPYTEKIILAVSQHPHMFENRASNYSFNISTQPTYQIISSDNLIDSTGLNRHFSGSQRMSIPHKYLHKPDIALSKNHTL
Sbjct:    1 MSSNQDIMEKRHAFTDCEEKYVCKVRRNDHNYRQVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNMLNSKEKFPLSKSPSLMQEQNRKRRLNSCSVDLEIAQIENQDSTLDFSNSKNSNYLSSLSQENSNSITCQASEKTPGSLEHLVAAIEQVEGEKFYVGSINNSEIPLQIMKKEINSAGSSVIELNDDSFENVDKHHHHHHHHHSNNPPPTPPSHPLHHTQQQMQSIQSISDTDNTPLLNSNADIKKQSNSNNNNNNNSVMKPTISPYTEKIILAVSQHPHMFENRASNYSFNISTQPTYQIISSDNLIDSTGLNRHFSGSQRMSIPHKYLHKPDIALSKNHTL 393
[Close]
BLAST of Transcription factor AP-4 vs. Planmine SMEST
Match: SMESG000081049.1 (SMESG000081049.1)

HSP 1 Score: 653.284 bits (1684), Expect = 0.000e+0
Identity = 386/386 (100.00%), Postives = 386/386 (100.00%), Query Frame = 2 
Query:  140 MEKRHAFTDCEEKYVCKVRRNDHNYRQVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNMLNSKEKFPLSKSPSLMQEQNRKRRLNSCSVDLEIAQIENQDSTLDFXXXXXXXXXXXXXQENSNSITCQASEKTPGSLEHLVAAIEQVEGEKFYVGSINNSEIPLQIMKKEINSAGSSVIELNDDSFENVDKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDTDNTPLLNSNADIKKQXXXXXXXXXXXVMKPTISPYTEKIILAVSQHPHMFENRASNYSFNISTQPTYQIISSDNLIDSTGLNRHFSGSQRMSIPHKYLHKPDIALSKNHTL 1297
            MEKRHAFTDCEEKYVCKVRRNDHNYRQVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNMLNSKEKFPLSKSPSLMQEQNRKRRLNSCSVDLEIAQIENQDSTLDFSNSKNSNYLSSLSQENSNSITCQASEKTPGSLEHLVAAIEQVEGEKFYVGSINNSEIPLQIMKKEINSAGSSVIELNDDSFENVDKHHHHHHHHHSNNPPPTPPSHPLHHTQQQMQSIQSISDTDNTPLLNSNADIKKQSNSNNNNNNNSVMKPTISPYTEKIILAVSQHPHMFENRASNYSFNISTQPTYQIISSDNLIDSTGLNRHFSGSQRMSIPHKYLHKPDIALSKNHTL
Sbjct:    1 MEKRHAFTDCEEKYVCKVRRNDHNYRQVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVACYKKNMLNSKEKFPLSKSPSLMQEQNRKRRLNSCSVDLEIAQIENQDSTLDFSNSKNSNYLSSLSQENSNSITCQASEKTPGSLEHLVAAIEQVEGEKFYVGSINNSEIPLQIMKKEINSAGSSVIELNDDSFENVDKHHHHHHHHHSNNPPPTPPSHPLHHTQQQMQSIQSISDTDNTPLLNSNADIKKQSNSNNNNNNNSVMKPTISPYTEKIILAVSQHPHMFENRASNYSFNISTQPTYQIISSDNLIDSTGLNRHFSGSQRMSIPHKYLHKPDIALSKNHTL 386
[Close]
BLAST of Transcription factor AP-4 vs. Planmine SMEST
Match: SMESG000062264.1 (SMESG000062264.1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.639e-13
Identity = 38/87 (43.68%), Postives = 52/87 (59.77%), Query Frame = 2 
Query:  119 MSSNQDIMEKRHAFTDCEEKY------VCKVRRNDHNYRQVYEYDKKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKA 361
            MS+  D +E+ +   D E K         K+ +ND         D K++RE+AN+NERRRMQSIN GFE+L++LLP   E EK+SK 
Sbjct:    1 MSTYSDKLERHYILGDLENKLKRKSSRTLKLLKNDD--------DIKIRREVANNNERRRMQSINAGFESLRKLLPFGTEKEKISKV 79
[Close]
BLAST of Transcription factor AP-4 vs. Planmine SMEST
Match: SMESG000018258.1 (SMESG000018258.1)

HSP 1 Score: 50.0618 bits (118), Expect = 1.192e-6
Identity = 35/99 (35.35%), Postives = 47/99 (47.47%), Query Frame = 2 
Query:  245 KREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRL------TSLEKEVAC---YKKNMLNSKEKFPLSKSPSLMQEQN 514
            +R  ANS ER RM+ ++  F  LK  LP   +  KLSK   L+  A YI  L   L      T+L    +    Y   M+NS  K P+ ++ S  Q QN
Sbjct:   40 QRSAANSRERSRMRVLSSAFIDLKSALPWVPKDTKLSKLDTLKLAAGYIAYLKSVLVEDFSGTTLPPPWSVYDIYSPYMVNSSNKCPIPRTWSPTQHQN 138
[Close]
BLAST of Transcription factor AP-4 vs. Planmine SMEST
Match: SMESG000018830.1 (SMESG000018830.1)

HSP 1 Score: 47.3654 bits (111), Expect = 7.631e-6
Identity = 22/55 (40.00%), Postives = 36/55 (65.45%), Query Frame = 2 
Query:  236 KKMKREIANSNERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSL 400
            ++++R  AN+ ER R+ +I+  FE+L+Q++P      KLSK  IL+    YI+SL
Sbjct:   75 RQVRRMEANARERNRVHTISSAFESLRQVVPSYYNSYKLSKLGILRIACCYIESL 129
The following BLAST results are available for this feature:
BLAST of Transcription factor AP-4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
TFAP41.640e-1569.64transcription factor AP-4 [Source:HGNC Symbol;Acc:... [more]
TFAP42.247e-870.00transcription factor AP-4 [Source:HGNC Symbol;Acc:... [more]
FIGLA9.590e-635.00folliculogenesis specific bHLH transcription facto... [more]
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BLAST of Transcription factor AP-4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hlh-117.586e-1460.00Helix-loop-helix protein 11 [Source:UniProtKB/Swi... [more]
hlh-117.586e-1460.00Helix-loop-helix protein 11 [Source:UniProtKB/Swi... [more]
hlh-117.691e-1460.00Helix-loop-helix protein 11 [Source:UniProtKB/Swi... [more]
hlh-117.691e-1460.00Helix-loop-helix protein 11 [Source:UniProtKB/Swi... [more]
hlh-111.126e-1263.64Helix-loop-helix protein 11 [Source:UniProtKB/Swi... [more]
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BLAST of Transcription factor AP-4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
crp2.219e-1559.70gene:FBgn0001994 transcript:FBtr0343814[more]
crp2.219e-1559.70gene:FBgn0001994 transcript:FBtr0080854[more]
ato3.946e-640.32gene:FBgn0010433 transcript:FBtr0081828[more]
cato6.598e-633.72gene:FBgn0024249 transcript:FBtr0087183[more]
tx7.695e-639.73gene:FBgn0263118 transcript:FBtr0085009[more]
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BLAST of Transcription factor AP-4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
tfap43.547e-1567.86transcription factor AP-4 (activating enhancer bin... [more]
atoh75.555e-637.29atonal bHLH transcription factor 7 [Source:NCBI ge... [more]
twist38.515e-638.75twist3 [Source:ZFIN;Acc:ZDB-GENE-000210-7][more]
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BLAST of Transcription factor AP-4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rbm281.283e-1569.64RNA binding motif protein 28 [Source:Xenbase;Acc:X... [more]
ppil63.802e-744.23peptidylprolyl isomerase like 6 [Source:Xenbase;Ac... [more]
st6gal16.022e-634.48ST6 beta-galactosamide alpha-2,6-sialyltranferase ... [more]
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BLAST of Transcription factor AP-4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Tfap42.848e-1669.64transcription factor AP4 [Source:MGI Symbol;Acc:MG... [more]
Tfap49.459e-1669.64transcription factor AP4 [Source:MGI Symbol;Acc:MG... [more]
Tfap46.289e-870.00transcription factor AP4 [Source:MGI Symbol;Acc:MG... [more]
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BLAST of Transcription factor AP-4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q01664|TFAP4_HUMAN7.876e-1569.64Transcription factor AP-4 OS=Homo sapiens OX=9606 ... [more]
sp|P34474|HLH11_CAEEL1.433e-1163.64Helix-loop-helix protein 11 OS=Caenorhabditis eleg... [more]
sp|O13126|ATO7B_XENLA5.918e-742.62Protein atonal homolog 7-B OS=Xenopus laevis OX=83... [more]
sp|Q10574|LIN32_CAEEL6.690e-744.44Protein lin-32 OS=Caenorhabditis elegans OX=6239 G... [more]
sp|O13125|ATO7A_XENLA5.579e-639.34Protein atonal homolog 7-A OS=Xenopus laevis OX=83... [more]
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BLAST of Transcription factor AP-4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4P0Q12.356e-2637.99Transcription factor AP-4 OS=Paragonimus westerman... [more]
A0A0X3Q3I62.362e-2636.40Transcription factor AP-4 OS=Schistocephalus solid... [more]
A0A183QI357.593e-2439.90BHLH domain-containing protein OS=Schistosoma rodh... [more]
A0A0R3WDZ88.555e-2436.56BHLH domain-containing protein OS=Taenia asiatica ... [more]
G4LWQ59.170e-2439.90Activator protein 4 (Ap4), putative OS=Schistosoma... [more]
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BLAST of Transcription factor AP-4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
tfap42.065e-1567.86transcription factor AP-4 [Source:NCBI gene;Acc:10... [more]
twist32.209e-638.75twist3 [Source:ZFIN;Acc:ZDB-GENE-000210-7][more]
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BLAST of Transcription factor AP-4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSPMAT00000004072.12.217e-636.25pep scaffold:Pmarinus_7.0:GL492732:1750:4325:-1 ge... [more]
TWIST12.380e-640.98twist family bHLH transcription factor 1 [Source:H... [more]
ENSPMAT00000002470.12.785e-640.98pep scaffold:Pmarinus_7.0:GL487131:18401:21594:-1 ... [more]
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BLAST of Transcription factor AP-4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor AP-4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO390157.713e-1767.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO461933.864e-847.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO362663.296e-637.04Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO461943.831e-645.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
NVATO26.762e-638.18Atonal-related protein 2; Predicted protein [Sour... [more]
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BLAST of Transcription factor AP-4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
TFAP41.221e-1569.64transcription factor AP-4 [Source:NCBI gene;Acc:10... [more]
tfap44.276e-1566.07transcription factor AP-4 [Source:NCBI gene;Acc:10... [more]
tfap42.452e-1063.83transcription factor AP-4 [Source:NCBI gene;Acc:10... [more]
atoh75.009e-734.29atonal bHLH transcription factor 7 [Source:NCBI ge... [more]
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BLAST of Transcription factor AP-4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000081049.10.000e+0100.00SMESG000081049.1[more]
SMESG000081049.10.000e+0100.00SMESG000081049.1[more]
SMESG000062264.11.639e-1343.68SMESG000062264.1[more]
SMESG000018258.11.192e-635.35SMESG000018258.1[more]
SMESG000018830.17.631e-640.00SMESG000018830.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033844 ID=SMED30033844|Name=Transcription factor AP-4|organism=Schmidtea mediterranea sexual|type=transcript|length=2109bp
TATCTTGACCTAATAAACAACTGTTTTTTGCAAATTATCCATTATCTAAT
AACTTAGTGATTTCGTTTTGTCTTTTATTGTTGTCACTCGGAGTTTTTAT
CAAATTTATTCATTCAAAATGTCAAGTAACCAAGATATTATGGAAAAACG
GCATGCCTTTACGGACTGTGAGGAAAAATACGTTTGCAAAGTTCGACGAA
ATGATCACAACTATCGTCAAGTGTATGAGTATGATAAGAAAATGAAGCGT
GAAATTGCCAACAGTAATGAAAGGCGTAGAATGCAAAGTATAAATATGGG
ATTCGAAACTCTCAAACAACTTTTGCCGATGACAATGGAAGGAGAAAAGC
TAAGCAAGGCCTCAATACTACAATTTACTGCAAAATATATCCAATCATTA
ATTGTTCGACTGACATCTCTGGAAAAAGAAGTAGCTTGCTATAAAAAGAA
CATGTTGAATAGCAAAGAAAAATTTCCATTATCTAAATCTCCATCATTGA
TGCAAGAACAAAATCGAAAAAGACGATTGAACAGTTGTAGTGTTGATTTA
GAAATTGCTCAAATTGAAAACCAAGATTCGACTTTAGATTTTTCCAATTC
TAAAAATTCAAATTATTTATCATCACTTAGTCAAGAGAATTCAAATTCAA
TAACCTGTCAAGCATCAGAAAAAACTCCAGGAAGTTTGGAGCATTTGGTA
GCGGCAATTGAACAAGTGGAGGGTGAAAAATTCTATGTTGGATCGATTAA
TAATAGTGAGATTCCATTGCAAATAATGAAAAAGGAAATCAATAGTGCTG
GTTCGTCGGTTATTGAATTAAATGATGATTCGTTTGAAAATGTAGATAAA
CACCACCACCACCACCATCATCATCATAGTAATAATCCTCCTCCTACTCC
TCCCTCCCATCCTCTCCACCATACCCAGCAGCAAATGCAATCCATACAAT
CAATATCAGACACAGATAATACACCTCTGCTCAACAGTAACGCCGATATC
AAAAAGCAATCCAATAGTAATAATAATAATAATAATAATTCTGTAATGAA
ACCAACAATAAGTCCATACACTGAAAAAATAATCCTAGCAGTTTCCCAGC
ACCCGCATATGTTTGAGAATCGGGCATCCAATTACAGTTTCAATATATCC
ACTCAACCAACCTATCAAATCATTTCTAGCGACAATCTGATCGACTCAAC
GGGATTAAATCGCCATTTCTCTGGATCTCAAAGAATGTCGATACCTCACA
AATACCTTCATAAACCGGATATTGCTCTATCAAAAAATCATACGTTATGA
TTTTTTTCGGTTGTCTTCGTTTATAATCTTTGTTTAGAATGTGTTTATTA
AGCATTGAAAAGTTTAGCCGGAATTCTTTACACATCAAGTCAACACAATA
GGAAACTAATCAGCTTGATAAAAACAATAAATCAATATTTATTAACATGT
ATAATAAACTCTGTTATTTTTTTTGTTTTTTTTTAATGTTATTCTTTTTC
GTTAAATTTGATTGGTTCAAAAATTAACTATTGAACGGGTTTGTAATTAA
ATAATTACTCAATTATGATTTTTGATTGGTAGATATTGAATTGGAAAATA
CAATTTTCTTTGAAAAAAAATCATATTTTGTTAGTGTTTTGATTTTATGC
AATCTTTATAATAAAAATGGAAGCAAAGGAAATTTTCCATTAATGAATTT
TGAGAATTGGGTTGACTCCTTCTTCATTCCAGTGATTTTTTTCTTTTTTC
ATTATCGAGATTGGATGCTGGCCAAATAAGGATAATTTTTCATTTATACC
TGTACAGGTGGAGAGTGATTGAAGGAATTGTCTATCTGATTTCAGACCTA
TAACTACTGTGTTTTATGTTGATATATTCTACTGCTCTTTTTTATTTCTC
ATGTAATCGTAATTTTACATGTAAATATTTTTTTAAATACTGTACTGTTG
TAATGTAATATTTCTGTTAGAGATTTTTGGAATTATGTTTGATGTATTAA
AAAGTGATGCTAATTTTTACAAATATCATAGACATTCATGACATTACCTT
CCCGGTTCTTGTTTTTGTTAACACTGTCATGATAGTCAATAAACATTTAT
TTACAGATC
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protein sequence of SMED30033844-orf-1

>SMED30033844-orf-1 ID=SMED30033844-orf-1|Name=SMED30033844-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=394bp
MSSNQDIMEKRHAFTDCEEKYVCKVRRNDHNYRQVYEYDKKMKREIANSN
ERRRMQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTS
LEKEVACYKKNMLNSKEKFPLSKSPSLMQEQNRKRRLNSCSVDLEIAQIE
NQDSTLDFSNSKNSNYLSSLSQENSNSITCQASEKTPGSLEHLVAAIEQV
EGEKFYVGSINNSEIPLQIMKKEINSAGSSVIELNDDSFENVDKHHHHHH
HHHSNNPPPTPPSHPLHHTQQQMQSIQSISDTDNTPLLNSNADIKKQSNS
NNNNNNNSVMKPTISPYTEKIILAVSQHPHMFENRASNYSFNISTQPTYQ
IISSDNLIDSTGLNRHFSGSQRMSIPHKYLHKPDIALSKNHTL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000103central nervous system
PLANA:0000120progenitor cell
PLANA:0000121stem cell
PLANA:0002111X2 cell
PLANA:0002142parenchyma
Vocabulary: INTERPRO
TermDefinition
IPR036638HLH_DNA-bd_sf
IPR011598bHLH_dom
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 91..118
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 243..278
NoneNo IPR availablePANTHERPTHR15741BASIC HELIX-LOOP-HELIX ZIP TRANSCRIPTION FACTORcoord: 35..94
NoneNo IPR availablePANTHERPTHR15741:SF27TRANSCRIPTION FACTOR AP-4coord: 35..94
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 48..100
e-value: 1.9E-15
score: 67.3
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 43..94
e-value: 2.8E-15
score: 56.0
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 42..94
score: 16.018
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainCDDcd00083HLHcoord: 40..94
e-value: 6.25821E-15
score: 66.8539
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3DG3DSA:4.10.280.10coord: 37..105
e-value: 1.6E-16
score: 61.5
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILYSSF47459HLH, helix-loop-helix DNA-binding domaincoord: 40..112