Sp5

Overview


Name	Sp5 (AIZ98420.1)
Smed ID	SMED30033839
Length (bp)	1336

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Sp5 (SMED30033839) t-SNE clustered cells

Violin plots show distribution of expression levels for Sp5 (SMED30033839) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Sp5 (SMED30033839) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Sp5 (SMED30033839) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30033839

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 19

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
posterior region of the whole animal	SMED30033839	SMESG000023227.1	dd_Smed_v4_7824_0_1	dd_Smed_v4	PMID:27063937 Scimone et al., 2016
posterior region of the whole animal	SMED30033839	SMESG000023227.1	dd_Smed_v4_7824_0_1	dd_Smed_v4	PMID:27063937 Scimone et al., 2016
tail region	SMED30033839	SMESG000023227.1	dd_Smed_v4_7824_0_1	dd_Smed_v4	PMID:27063937 Scimone et al., 2016
tail region	SMED30033839	SMESG000023227.1	SmedASXL_006379	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
Category 2 cell	SMED30033839	SMESG000023227.1	SMED30033839	smed_20140614	PMID:29100657 Cheng et al., 2018
epidermis	SMED30033839	SMESG000023227.1	SMED30033839	smed_20140614	PMID:29100657 Cheng et al., 2018
Category 3 cell	SMED30033839	SMESG000023227.1	SMED30033839	smed_20140614	PMID:29100657 Cheng et al., 2018
tail region	SMED30033839	SMESG000023227.1	SMED30033839	smed_20140614	PMID:29100657 Cheng et al., 2018
neoblast	SMED30033839	SMESG000023227.1	SMED30033839	smed_20140614	PMID:29100657 Cheng et al., 2018
X1 cell	SMED30033839	SMESG000023227.1	SMED30033839	smed_20140614	PMID:29100657 Cheng et al., 2018
nervous system	SMED30033839	SMESG000023227.1	dd_Smed_v4_7824_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30033839	SMESG000023227.1	dd_Smed_v4_7824_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30033839	SMESG000023227.1	dd_Smed_v4_7824_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
posterior side	SMED30033839	SMESG000023227.1	dd_Smed_v4_7824_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
posterior muscle cell	SMED30033839	SMESG000023227.1	dd_Smed_v4_7824_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
tail region	SMED30033839	SMESG000023227.1	dd_Smed_v6_7824_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
reproductive organ	SMED30033839	SMESG000023227.1	Contig49437	newmark_ests	PMID:28434803 Rouhana et al., 2017
reproductive organ	SMED30033839	SMESG000023227.1	Contig49437	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
cement gland	SMED30033839	SMESG000023227.1	KP003818.1	smed_ncbi_20200123	PMID:279364

Note: Hover over icons to view figure legend

Homology

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BLAST of Sp5 vs. Ensembl Human
Match: SP3 (Sp3 transcription factor [Source:HGNC Symbol;Acc:HGNC:11208])

HSP 1 Score: 169.859 bits (429), Expect = 5.665e-46
Identity = 74/106 (69.81%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +G     NL G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPFVC +  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  507 CTCPNCKEGGGRGTNL-GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 611

[Close]

BLAST of Sp5 vs. Ensembl Human
Match: SP3 (Sp3 transcription factor [Source:HGNC Symbol;Acc:HGNC:11208])

HSP 1 Score: 170.629 bits (431), Expect = 7.406e-46
Identity = 74/106 (69.81%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +G     NL G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPFVC +  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  557 CTCPNCKEGGGRGTNL-GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 661

[Close]

BLAST of Sp5 vs. Ensembl Human
Match: SP3 (Sp3 transcription factor [Source:HGNC Symbol;Acc:HGNC:11208])

HSP 1 Score: 170.629 bits (431), Expect = 7.490e-46
Identity = 74/106 (69.81%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +G     NL G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPFVC +  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  532 CTCPNCKEGGGRGTNL-GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 636

[Close]

BLAST of Sp5 vs. Ensembl Human
Match: SP3 (Sp3 transcription factor [Source:HGNC Symbol;Acc:HGNC:11208])

HSP 1 Score: 170.629 bits (431), Expect = 7.946e-46
Identity = 74/106 (69.81%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +G     NL G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPFVC +  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  532 CTCPNCKEGGGRGTNL-GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 636

[Close]

BLAST of Sp5 vs. Ensembl Human
Match: SP3 (Sp3 transcription factor [Source:HGNC Symbol;Acc:HGNC:11208])

HSP 1 Score: 170.629 bits (431), Expect = 1.171e-45
Identity = 74/106 (69.81%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +G     NL G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPFVC +  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  600 CTCPNCKEGGGRGTNL-GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 704

[Close]

BLAST of Sp5 vs. Ensembl Celegans
Match: sptf-3 (SP (Specificity Protein) Transcription Factor [Source:UniProtKB/TrEMBL;Acc:Q9XW26])

HSP 1 Score: 147.517 bits (371), Expect = 1.039e-39
Identity = 69/132 (52.27%), Postives = 92/132 (69.70%), Query Frame = 3

Query:  696 NISTQLNLSSNRANIPQLGQRKCQK---CTCPNCHQGLNSRANLNGAKKVHICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            N    +N+ ++  +I   G  +  K   CTCPNC Q  N + +  G  ++HIC++C+KTYGKTSHL+AH+R H  ++PF C +  C+K FTRSDELQRH RTHTGEKRF C  C K+FMRSDHL KH++TH+
Sbjct:  268 NEDGMMNMGNDYGDINGQGLGEAPKRCACTCPNCLQQNNRQGD--GKSRIHICHLCNKTYGKTSHLRAHLRGHAGNKPFACDWQHCNKKFTRSDELQRHRRTHTGEKRFACNHCGKKFMRSDHLTKHERTHQ 397

[Close]

BLAST of Sp5 vs. Ensembl Celegans
Match: sptf-2 (SP (Specificity Protein) Transcription Factor [Source:UniProtKB/TrEMBL;Acc:Q22678])

HSP 1 Score: 115.931 bits (289), Expect = 1.798e-30
Identity = 58/114 (50.88%), Postives = 73/114 (64.04%), Query Frame = 3

Query:  732 ANIPQLGQRKCQKCTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKH 1067
            AN     +RKC++CTCPNC      +    G++  H+C +  C KTY KTSHL+AH+R H  DRPFVC +  C K F RSD+L RH RTHT E RF C  C ++F RSDHL +H
Sbjct:   45 ANELTTKRRKCERCTCPNCKA---IKHGDRGSQHTHLCSVPGCGKTYKKTSHLRAHLRKHTGDRPFVCDWFDCGKRFDRSDQLIRHKRTHTKEYRFACKFCIRQFSRSDHLQQH 155

[Close]

BLAST of Sp5 vs. Ensembl Celegans
Match: klf-3 (Kruppel-Like Factor (Zinc finger protein) [Source:UniProtKB/TrEMBL;Acc:P91329])

HSP 1 Score: 98.5969 bits (244), Expect = 7.596e-23
Identity = 50/98 (51.02%), Postives = 60/98 (61.22%), Query Frame = 3

Query:  801 NSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGV 1088
            N R   +    VH C    C K Y K+SHLKAH R H+ ++PFVC +  CS  F RSDEL RHMR HTG+K F C LC + F RSDHL+ H K H  +
Sbjct:  212 NKRNPTDKKFVVHACTYPGCFKKYSKSSHLKAHERTHSGEKPFVCKWQNCSWKFARSDELTRHMRKHTGDKPFRCSLCDRNFARSDHLSLHMKRHSTI 309

[Close]

BLAST of Sp5 vs. Ensembl Celegans
Match: klf-3 (Kruppel-Like Factor (Zinc finger protein) [Source:UniProtKB/TrEMBL;Acc:P91329])

HSP 1 Score: 98.5969 bits (244), Expect = 7.805e-23
Identity = 50/98 (51.02%), Postives = 60/98 (61.22%), Query Frame = 3

Query:  801 NSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGV 1088
            N R   +    VH C    C K Y K+SHLKAH R H+ ++PFVC +  CS  F RSDEL RHMR HTG+K F C LC + F RSDHL+ H K H  +
Sbjct:  218 NKRNPTDKKFVVHACTYPGCFKKYSKSSHLKAHERTHSGEKPFVCKWQNCSWKFARSDELTRHMRKHTGDKPFRCSLCDRNFARSDHLSLHMKRHSTI 315

[Close]

BLAST of Sp5 vs. Ensembl Celegans
Match: klf-2 (Kruppel-Like Factor (Zinc finger protein) [Source:UniProtKB/TrEMBL;Acc:O62259])

HSP 1 Score: 89.7373 bits (221), Expect = 9.111e-20
Identity = 44/86 (51.16%), Postives = 54/86 (62.79%), Query Frame = 3

Query:  831 KVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            +VH C+   C K Y K+SHL AH R H+ ++P+ C +  CS  F RSDEL RH R HTG K F C  C ++F RSDHL  H K HE
Sbjct:  196 RVHKCFYQGCGKVYTKSSHLTAHERVHSGEKPYPCEWPGCSWRFARSDELTRHYRKHTGAKPFACKECSRKFSRSDHLQLHMKRHE 281

HSP 2 Score: 54.6842 bits (130), Expect = 4.843e-8
Identity = 26/60 (43.33%), Postives = 32/60 (53.33%), Query Frame = 3

Query:  927 CTYHLCSKSFTRSDELQRHMRTHTGEKRFVC--PLCFKRFMRSDHLAKHKKTHEGVDPIV 1100
            C Y  C K +T+S  L  H R H+GEK + C  P C  RF RSD L +H + H G  P  
Sbjct:  200 CFYQGCGKVYTKSSHLTAHERVHSGEKPYPCEWPGCSWRFARSDELTRHYRKHTGAKPFA 259

HSP 3 Score: 47.7506 bits (112), Expect = 7.560e-6
Identity = 32/117 (27.35%), Postives = 51/117 (43.59%), Query Frame = 3

Query:  717 LSSNRANIPQLGQRKCQKCTCPNCHQGLNSRANLNGAKKVHICYI--------CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFM 1043
            + S R +   L + +  KC    C +     ++L   ++VH            C   + ++  L  H R H   +PF C    CS+ F+RSD LQ HM+ H  +++      FK FM
Sbjct:  182 VPSKRRDKATLDRLRVHKCFYQGCGKVYTKSSHLTAHERVHSGEKPYPCEWPGCSWRFARSDELTRHYRKHTGAKPFACK--ECSRKFSRSDHLQLHMKRHETDEQDDGMDDFKDFM 296

[Close]

BLAST of Sp5 vs. Ensembl Fly
Match: Spps (gene:FBgn0039169 transcript:FBtr0084565)

HSP 1 Score: 155.221 bits (391), Expect = 1.818e-40
Identity = 76/136 (55.88%), Postives = 92/136 (67.65%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTH----EGVDPIVLGTVISKDTNSSIKSPKALN 1160
            CTCPNC  G          K+ HIC+I  CHK YGKTSHL+AH+RWH  +RPFVC++  C K FTRSDELQRH RTHTGEKRF C  C K+FMRSDHL+KH KTH     GV+ I L   I ++T     +PK+++
Sbjct:  722 CTCPNCTDGEKHSDK----KRQHICHITGCHKVYGKTSHLRAHLRWHTGERPFVCSWAFCGKRFTRSDELQRHRRTHTGEKRFQCQECNKKFMRSDHLSKHIKTHFKSRSGVELIELS--IKQETKGG-NAPKSIS 850

[Close]

BLAST of Sp5 vs. Ensembl Fly
Match: Sp1 (gene:FBgn0020378 transcript:FBtr0071381)

HSP 1 Score: 151.754 bits (382), Expect = 2.237e-40
Identity = 71/112 (63.39%), Postives = 79/112 (70.54%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGA----KKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGV 1088
            C CPNC +    R    G     K +H C+I  C K YGKTSHLKAH+RWH  +RPFVC +  C K FTRSDELQRH+RTHTGEKRF CP+C KRFMRSDHLAKH KTH G 
Sbjct:  166 CDCPNCQEA--ERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHNGT 275

[Close]

BLAST of Sp5 vs. Ensembl Fly
Match: Spps (gene:FBgn0039169 transcript:FBtr0330382)

HSP 1 Score: 154.836 bits (390), Expect = 2.308e-40
Identity = 76/136 (55.88%), Postives = 93/136 (68.38%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTH----EGVDPIVLGTVISKDTNSSIKSPKALN 1160
            CTCPNC  G       +  K+ HIC+I  CHK YGKTSHL+AH+RWH  +RPFVC++  C K FTRSDELQRH RTHTGEKRF C  C K+FMRSDHL+KH KTH     GV+ I L   I ++T     +PK+++
Sbjct:  722 CTCPNCTDGEKH----SDKKRQHICHITGCHKVYGKTSHLRAHLRWHTGERPFVCSWAFCGKRFTRSDELQRHRRTHTGEKRFQCQECNKKFMRSDHLSKHIKTHFKSRSGVELIELS--IKQETKGG-NAPKSIS 850

[Close]

BLAST of Sp5 vs. Ensembl Fly
Match: Sp1 (gene:FBgn0020378 transcript:FBtr0273354)

HSP 1 Score: 152.91 bits (385), Expect = 5.040e-40
Identity = 71/112 (63.39%), Postives = 79/112 (70.54%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGA----KKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGV 1088
            C CPNC +    R    G     K +H C+I  C K YGKTSHLKAH+RWH  +RPFVC +  C K FTRSDELQRH+RTHTGEKRF CP+C KRFMRSDHLAKH KTH G 
Sbjct:  361 CDCPNCQEA--ERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHNGT 470

[Close]

BLAST of Sp5 vs. Ensembl Fly
Match: Sp1 (gene:FBgn0020378 transcript:FBtr0331335)

HSP 1 Score: 153.295 bits (386), Expect = 5.139e-40
Identity = 71/112 (63.39%), Postives = 79/112 (70.54%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGA----KKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGV 1088
            C CPNC +    R    G     K +H C+I  C K YGKTSHLKAH+RWH  +RPFVC +  C K FTRSDELQRH+RTHTGEKRF CP+C KRFMRSDHLAKH KTH G 
Sbjct:  361 CDCPNCQEA--ERLGPAGVHLRKKNIHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTHNGT 470

[Close]

BLAST of Sp5 vs. Ensembl Zebrafish
Match: sp3a (sp3a transcription factor [Source:ZFIN;Acc:ZDB-GENE-070410-111])

HSP 1 Score: 169.859 bits (429), Expect = 7.485e-46
Identity = 72/106 (67.92%), Postives = 84/106 (79.25%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +G    +N+ G KK H+C+I  C K YGKTSHL+AH+RWH+ +RPFVCT+  C K FTRSDELQRH RTHTGEK+FVC  C KRFMRSDHLAKH KTH+
Sbjct:  512 CTCPNCKEGGGRGSNM-GKKKQHVCHIAGCGKVYGKTSHLRAHLRWHSGERPFVCTWMFCGKRFTRSDELQRHRRTHTGEKKFVCSECSKRFMRSDHLAKHIKTHQ 616

[Close]

BLAST of Sp5 vs. Ensembl Zebrafish
Match: sp3a (sp3a transcription factor [Source:ZFIN;Acc:ZDB-GENE-070410-111])

HSP 1 Score: 169.474 bits (428), Expect = 7.619e-46
Identity = 72/106 (67.92%), Postives = 84/106 (79.25%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +G    +N+ G KK H+C+I  C K YGKTSHL+AH+RWH+ +RPFVCT+  C K FTRSDELQRH RTHTGEK+FVC  C KRFMRSDHLAKH KTH+
Sbjct:  500 CTCPNCKEGGGRGSNM-GKKKQHVCHIAGCGKVYGKTSHLRAHLRWHSGERPFVCTWMFCGKRFTRSDELQRHRRTHTGEKKFVCSECSKRFMRSDHLAKHIKTHQ 604

[Close]

BLAST of Sp5 vs. Ensembl Zebrafish
Match: sp3b (Sp3b transcription factor [Source:ZFIN;Acc:ZDB-GENE-110815-3])

HSP 1 Score: 167.548 bits (423), Expect = 2.460e-45
Identity = 71/106 (66.98%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +    R +  G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPF+C++  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  469 CTCPNCKEA-GGRGSSMGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFICSWSYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 573

[Close]

BLAST of Sp5 vs. Ensembl Zebrafish
Match: sp3b (Sp3b transcription factor [Source:ZFIN;Acc:ZDB-GENE-110815-3])

HSP 1 Score: 167.548 bits (423), Expect = 2.852e-45
Identity = 71/106 (66.98%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +    R +  G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPF+C++  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  481 CTCPNCKEA-GGRGSSMGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFICSWSYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 585

[Close]

BLAST of Sp5 vs. Ensembl Zebrafish
Match: sp3b (Sp3b transcription factor [Source:ZFIN;Acc:ZDB-GENE-110815-3])

HSP 1 Score: 166.007 bits (419), Expect = 1.266e-44
Identity = 71/106 (66.98%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +    R +  G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPF+C++  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  493 CTCPNCKEA-GGRGSSMGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFICSWSYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 597

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BLAST of Sp5 vs. Ensembl Xenopus
Match: zp3.2 (zona pellucida glycoprotein 3 (sperm receptor), gene 2 [Source:Xenbase;Acc:XB-GENE-1019608])

HSP 1 Score: 171.014 bits (432), Expect = 2.522e-46
Identity = 75/106 (70.75%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC  G     NL G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPFVCT+  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  484 CTCPNCKDGAGRGTNL-GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCTWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 588

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BLAST of Sp5 vs. Ensembl Xenopus
Match: zp3.2 (zona pellucida glycoprotein 3 (sperm receptor), gene 2 [Source:Xenbase;Acc:XB-GENE-1019608])

HSP 1 Score: 171.785 bits (434), Expect = 3.139e-46
Identity = 75/106 (70.75%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC  G     NL G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPFVCT+  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  551 CTCPNCKDGAGRGTNL-GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCTWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 655

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BLAST of Sp5 vs. Ensembl Xenopus
Match: nr2e3 (nuclear receptor subfamily 2 group E member 3 [Source:Xenbase;Acc:XB-GENE-485801])

HSP 1 Score: 171.014 bits (432), Expect = 5.699e-46
Identity = 81/147 (55.10%), Postives = 98/147 (66.67%), Query Frame = 3

Query:  741 PQLGQR-KCQKCTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE---GVDPIVLGTVISKDTNSSIKSPKALNP 1163
            PQ G+R + + CTCP C +G    +   G KK HIC+I  C K YGKTSHL+AH+RWH  +RPFVCT+  C K FTRSDELQRH RTHTGEK+F+CP C KRFMRSDHL+KH KTH+   G    V  T ++ DT +      A  P
Sbjct:  535 PQPGRRMRREACTCPYCKEGEGRGSGDPGKKKQHICHIPGCGKVYGKTSHLRAHLRWHTGERPFVCTWVFCGKRFTRSDELQRHKRTHTGEKKFICPECPKRFMRSDHLSKHIKTHQNKKGQGGPVAMTTVNLDTGAGSDGSAASTP 681

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BLAST of Sp5 vs. Ensembl Xenopus
Match: nr2e3 (nuclear receptor subfamily 2 group E member 3 [Source:Xenbase;Acc:XB-GENE-485801])

HSP 1 Score: 170.244 bits (430), Expect = 1.070e-45
Identity = 81/147 (55.10%), Postives = 98/147 (66.67%), Query Frame = 3

Query:  741 PQLGQR-KCQKCTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE---GVDPIVLGTVISKDTNSSIKSPKALNP 1163
            PQ G+R + + CTCP C +G    +   G KK HIC+I  C K YGKTSHL+AH+RWH  +RPFVCT+  C K FTRSDELQRH RTHTGEK+F+CP C KRFMRSDHL+KH KTH+   G    V  T ++ DT +      A  P
Sbjct:  554 PQPGRRMRREACTCPYCKEGEGRGSGDPGKKKQHICHIPGCGKVYGKTSHLRAHLRWHTGERPFVCTWVFCGKRFTRSDELQRHKRTHTGEKKFICPECPKRFMRSDHLSKHIKTHQNKKGQGGPVAMTTVNLDTGAGSDGSAASTP 700

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BLAST of Sp5 vs. Ensembl Xenopus
Match: fxr1 (FMR1 autosomal homolog 1 [Source:Xenbase;Acc:XB-GENE-1004708])

HSP 1 Score: 167.162 bits (422), Expect = 1.702e-44
Identity = 76/127 (59.84%), Postives = 88/127 (69.29%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEG--VDPIVLGTVISKDTNSSI 1139
            C+CPNC +G     +  G KK HIC+I  C K YGKTSHL+AH+RWH  +RPFVC +  C K FTRSDELQRH RTHTGEKRF CP C KRFMRSDHL+KH KTH+        L  V S D +SS+
Sbjct:  616 CSCPNCREGEGRGNSEPGKKKQHICHIENCGKVYGKTSHLRAHLRWHTGERPFVCNWIFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTHQNKKGGGTALAIVTSGDLDSSV 742

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BLAST of Sp5 vs. Ensembl Mouse
Match: Sp3 (trans-acting transcription factor 3 [Source:MGI Symbol;Acc:MGI:1277166])

HSP 1 Score: 170.629 bits (431), Expect = 6.807e-46
Identity = 73/106 (68.87%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +G     NL G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPF+C +  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  558 CTCPNCKEGGGRGTNL-GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 662

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BLAST of Sp5 vs. Ensembl Mouse
Match: Sp3 (trans-acting transcription factor 3 [Source:MGI Symbol;Acc:MGI:1277166])

HSP 1 Score: 170.244 bits (430), Expect = 1.037e-45
Identity = 73/106 (68.87%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +G     NL G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPF+C +  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  602 CTCPNCKEGGGRGTNL-GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 706

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BLAST of Sp5 vs. Ensembl Mouse
Match: Sp4 (trans-acting transcription factor 4 [Source:MGI Symbol;Acc:MGI:107595])

HSP 1 Score: 167.162 bits (422), Expect = 1.251e-44
Identity = 73/127 (57.48%), Postives = 89/127 (70.08%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEG--VDPIVLGTVISKDTNSSI 1139
            C+CPNC +G    ++  G KK H+C+I  C K YGKTSHL+AH+RWH  +RPF+C +  C K FTRSDELQRH RTHTGEKRF CP C KRFMRSDHL+KH KTH+        L  V S + +SS+
Sbjct:  623 CSCPNCREGEGRGSSEPGKKKQHVCHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTHQNKKGGGTALAIVTSGELDSSV 749

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BLAST of Sp5 vs. Ensembl Mouse
Match: Sp4 (trans-acting transcription factor 4 [Source:MGI Symbol;Acc:MGI:107595])

HSP 1 Score: 164.851 bits (416), Expect = 8.417e-44
Identity = 74/127 (58.27%), Postives = 88/127 (69.29%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEG--VDPIVLGTVISKDTNSSI 1139
            C+CPNC +G  S     G KK H+C+I  C K YGKTSHL+AH+RWH  +RPF+C +  C K FTRSDELQRH RTHTGEKRF CP C KRFMRSDHL+KH KTH+        L  V S + +SS+
Sbjct:  623 CSCPNCREGEGSSEP--GKKKQHVCHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTHQNKKGGGTALAIVTSGELDSSV 747

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BLAST of Sp5 vs. Ensembl Mouse
Match: Sp1 (trans-acting transcription factor 1 [Source:MGI Symbol;Acc:MGI:98372])

HSP 1 Score: 158.303 bits (399), Expect = 5.659e-43
Identity = 78/156 (50.00%), Postives = 98/156 (62.82%), Query Frame = 3

Query:  675 SVRNSPPNISTQLNL----------------SSNRANI-PQLGQR-KCQKCTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            ++ N+P +  TQL L                  N +++ PQ G+R + + CTCP C       +   G KK HIC+I  C K YGKTSHL+AH+RWH  +RPF+C +  C K FTRSDELQRH RTHTGEK+F CP C KRFMRSDHL+KH KTH+
Sbjct:  238 AIANTPGDHGTQLGLHGSGGDGIHDETAGGEGENSSDLQPQAGRRTRREACTCPYCKDSEGRASGDPGKKKQHICHIQGCGKVYGKTSHLRAHLRWHTGERPFMCNWSYCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQ 393

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BLAST of Sp5 vs. UniProt/SwissProt
Match: sp|Q90WR8|SP3_CHICK (Transcription factor Sp3 OS=Gallus gallus OX=9031 GN=SP3 PE=1 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 3.484e-45
Identity = 74/106 (69.81%), Postives = 84/106 (79.25%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +G    +NL G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPFVC +  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  590 CTCPNCKEGGGRGSNL-GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 694

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BLAST of Sp5 vs. UniProt/SwissProt
Match: sp|Q02447|SP3_HUMAN (Transcription factor Sp3 OS=Homo sapiens OX=9606 GN=SP3 PE=1 SV=3)

HSP 1 Score: 170.629 bits (431), Expect = 5.625e-45
Identity = 74/106 (69.81%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +G     NL G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPFVC +  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  600 CTCPNCKEGGGRGTNL-GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 704

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BLAST of Sp5 vs. UniProt/SwissProt
Match: sp|O70494|SP3_MOUSE (Transcription factor Sp3 OS=Mus musculus OX=10090 GN=Sp3 PE=1 SV=2)

HSP 1 Score: 170.244 bits (430), Expect = 7.257e-45
Identity = 73/106 (68.87%), Postives = 83/106 (78.30%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +G     NL G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPF+C +  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  602 CTCPNCKEGGGRGTNL-GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 706

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BLAST of Sp5 vs. UniProt/SwissProt
Match: sp|Q02446|SP4_HUMAN (Transcription factor Sp4 OS=Homo sapiens OX=9606 GN=SP4 PE=1 SV=2)

HSP 1 Score: 169.859 bits (429), Expect = 1.066e-44
Identity = 75/127 (59.06%), Postives = 89/127 (70.08%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEG--VDPIVLGTVISKDTNSSI 1139
            C+CPNC +G    +N  G KK HIC+I  C K YGKTSHL+AH+RWH  +RPF+C +  C K FTRSDELQRH RTHTGEKRF CP C KRFMRSDHL+KH KTH+        L  V S + +SS+
Sbjct:  625 CSCPNCREGEGRGSNEPGKKKQHICHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTHQNKKGGGTALAIVTSGELDSSV 751

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BLAST of Sp5 vs. UniProt/SwissProt
Match: sp|Q62445|SP4_MOUSE (Transcription factor Sp4 OS=Mus musculus OX=10090 GN=Sp4 PE=2 SV=2)

HSP 1 Score: 167.162 bits (422), Expect = 9.924e-44
Identity = 73/127 (57.48%), Postives = 89/127 (70.08%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEG--VDPIVLGTVISKDTNSSI 1139
            C+CPNC +G    ++  G KK H+C+I  C K YGKTSHL+AH+RWH  +RPF+C +  C K FTRSDELQRH RTHTGEKRF CP C KRFMRSDHL+KH KTH+        L  V S + +SS+
Sbjct:  623 CSCPNCREGEGRGSSEPGKKKQHVCHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTHQNKKGGGTALAIVTSGELDSSV 749

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BLAST of Sp5 vs. TrEMBL
Match: A0A0A7NZ65 (Sp5 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 596.275 bits (1536), Expect = 0.000e+0
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 3

Query:  273 MNYIPNQTNCSTRFLMNSNDFWQSPEMAALQRTTEKFFVNQSLINNPTNPSAXXXXXXXXXXXXXXXARKPCIELPSLIDSKNQMQLMNSLSSHALEDCVDHFFRRSINCFSSGYNLPNLMACKEFSLDFPSADSVRNSPPNISTQLNLSSNRANIPQLGQRKCQKCTCPNCHQGLNSRANLNGAKKVHICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLGTVISKDTNSSIKSPKALNPDKIYKKQQ 1187
            MNYIPNQTNCSTRFLMNSNDFWQSPEMAALQRTTEKFFVNQSLINNPTNPSAIPNPTNCPTTPIRPIARKPCIELPSLIDSKNQMQLMNSLSSHALEDCVDHFFRRSINCFSSGYNLPNLMACKEFSLDFPSADSVRNSPPNISTQLNLSSNRANIPQLGQRKCQKCTCPNCHQGLNSRANLNGAKKVHICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLGTVISKDTNSSIKSPKALNPDKIYKKQQ
Sbjct:    1 MNYIPNQTNCSTRFLMNSNDFWQSPEMAALQRTTEKFFVNQSLINNPTNPSAIPNPTNCPTTPIRPIARKPCIELPSLIDSKNQMQLMNSLSSHALEDCVDHFFRRSINCFSSGYNLPNLMACKEFSLDFPSADSVRNSPPNISTQLNLSSNRANIPQLGQRKCQKCTCPNCHQGLNSRANLNGAKKVHICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLGTVISKDTNSSIKSPKALNPDKIYKKQQ 305

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BLAST of Sp5 vs. TrEMBL
Match: A0A224AB84 (Sp5 OS=Dugesia japonica OX=6161 PE=2 SV=1)

HSP 1 Score: 592.808 bits (1527), Expect = 0.000e+0
Identity = 303/377 (80.37%), Postives = 334/377 (88.59%), Query Frame = 3

Query:  150 MNSGPAIINIPTSIHVLPSSPGYFSSKSHHLLSGPISPNIDMNYIPNQTNCSTRFLMNSNDFWQSPEMAALQRTTEKFFVNQSLINNPTNPSAXXXXXXXXXXXXXXXARKPCIELPSLIDSKNQMQLMNSLSSHALEDCVDHFFRRSINCFSSGYNLPNLMACKEFSLDFPSADSVRNSPPN-------ISTQ--LNLSSNRANIPQLGQRKCQKCTCPNCHQGLNSRANLNGAKKVHICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLGTVISKDTNSSIKSPKALNPDKIYKKQQNVKKPRLGLSVSNSAMNSPDHH 1253
            MNSGPAIINIPT+IH LP+SPGYFS+KS H+LSGPISPN+D+NYI + TNCS+RF+M+SNDFWQSPEMAALQRTTEKFFVNQSLI+N +NP+       C  TPIRPIARKPCIELPSLIDSK+Q+ L+NSLSS  +EDCVDHFFRRSINCFSS Y LPNL+ACK+F+L+FPS ++ RN+  N       +S Q  + +S NR NIPQ+GQRKCQKCTCPNCHQGLNSRANLNGAKKVHICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLGTVISKDTNSSIKS K LN DK YKKQQN+KKPR GLS +NS +NSPDHH
Sbjct:    1 MNSGPAIINIPTTIHGLPNSPGYFSNKSQHILSGPISPNLDLNYIQSHTNCSSRFVMSSNDFWQSPEMAALQRTTEKFFVNQSLISNNSNPNNSITTNICQNTPIRPIARKPCIELPSLIDSKSQIHLLNSLSSRTIEDCVDHFFRRSINCFSSPYTLPNLLACKDFNLEFPSVENTRNNNNNSPNISGLLSHQKPMTISPNRTNIPQIGQRKCQKCTCPNCHQGLNSRANLNGAKKVHICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLGTVISKDTNSSIKSEKTLNSDKTYKKQQNIKKPRPGLSATNSTINSPDHH 377

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BLAST of Sp5 vs. TrEMBL
Match: A0A448XCY8 (Uncharacterized protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS27380 PE=4 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 3.867e-49
Identity = 89/137 (64.96%), Postives = 98/137 (71.53%), Query Frame = 3

Query:  735 NIPQLGQRKCQKCTCPNCHQGLNSR------------ANLNGAKKVHICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE---G-VDPI 1097
            + P    RKCQKCTCPNC  G NSR              +   +KVHIC IC KTYGKTSHLKAH+RWHN++RPF C Y  C K+FTRSDELQRHMRTHTGEKRF C LC KRFMRSDHL+KHKKTHE   G VDP+
Sbjct:  593 STPLGAGRKCQKCTCPNCEVGRNSRCGPASGSRASAGPGVGPGRKVHICAICGKTYGKTSHLKAHVRWHNDERPFRCVYDFCDKAFTRSDELQRHMRTHTGEKRFACNLCEKRFMRSDHLSKHKKTHELATGLVDPV 729

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BLAST of Sp5 vs. TrEMBL
Match: A0A5E4Q5Z4 (Uncharacterized protein OS=Leptidea sinapis OX=189913 GN=LSINAPIS_LOCUS4920 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 8.204e-46
Identity = 89/175 (50.86%), Postives = 109/175 (62.29%), Query Frame = 3

Query:  753 QRKCQKCTCPNC---HQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLGTVISKDTNSSIKSPKALNPDKIYKKQQ----NVKKPRLGLSVSNSAMNSPDH 1250
            QR+C KC CPNC     G       +GAK+ H+C++  C K YGKTSHLKAH+RWH  +RPFVC +  C K FTRSDELQRH+RTHTGEKRF C LC KRFMRSDHLAKH KTH                N + KS KA   DK+  +Q      V++P  GL+   +A+ SP++
Sbjct:   75 QRRCAKCRCPNCLTEAAGFGPNYGKDGAKREHVCHVPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACQLCTKRFMRSDHLAKHVKTH---------------ANGTRKSKKAKEDDKVVIEQTKTSVEVEQPS-GLNSEATAVGSPNY 233

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BLAST of Sp5 vs. TrEMBL
Match: A0A087VHY4 (Transcription factor Sp3 (Fragment) OS=Balearica regulorum gibbericeps OX=100784 GN=N312_05345 PE=4 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 6.926e-45
Identity = 76/106 (71.70%), Postives = 86/106 (81.13%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            CTCPNC +G   R+NL G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPFVCT+  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  509 CTCPNCKEGGGRRSNL-GKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCTWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 613

[Close]

BLAST of Sp5 vs. Ensembl Cavefish
Match: sp3a (Sp3 transcription factor [Source:NCBI gene;Acc:103031127])

HSP 1 Score: 170.244 bits (430), Expect = 3.327e-46
Identity = 77/128 (60.16%), Postives = 92/128 (71.88%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE---GVDPIVLGTVISKDTNSSI 1139
            CTCPNC +G   R    G KK H+C+I  C K YGKTSHL+AH+RWH+ +RPFVC +  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+   G    V+ T+ +  ++ SI
Sbjct:  492 CTCPNCKEG-GGRGTSIGKKKQHVCHIVGCGKVYGKTSHLRAHLRWHSGERPFVCNWMFCGKRFTRSDELQRHRRTHTGEKKFVCPQCSKRFMRSDHLAKHIKTHQNKKGGGGTVVATMDTSASSDSI 618

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BLAST of Sp5 vs. Ensembl Cavefish
Match: sp3a (Sp3 transcription factor [Source:NCBI gene;Acc:103031127])

HSP 1 Score: 170.244 bits (430), Expect = 4.844e-46
Identity = 77/128 (60.16%), Postives = 92/128 (71.88%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE---GVDPIVLGTVISKDTNSSI 1139
            CTCPNC +G   R    G KK H+C+I  C K YGKTSHL+AH+RWH+ +RPFVC +  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+   G    V+ T+ +  ++ SI
Sbjct:  528 CTCPNCKEG-GGRGTSIGKKKQHVCHIVGCGKVYGKTSHLRAHLRWHSGERPFVCNWMFCGKRFTRSDELQRHRRTHTGEKKFVCPQCSKRFMRSDHLAKHIKTHQNKKGGGGTVVATMDTSASSDSI 654

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BLAST of Sp5 vs. Ensembl Cavefish
Match: sp3b (transcription factor Sp3-like [Source:NCBI gene;Acc:103030342])

HSP 1 Score: 167.162 bits (422), Expect = 3.605e-45
Identity = 71/105 (67.62%), Postives = 82/105 (78.10%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTH 1079
            CTCPNC +    R +  G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPF+C++  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH
Sbjct:  490 CTCPNCKEA-GGRGSGTGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFICSWSYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTH 593

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BLAST of Sp5 vs. Ensembl Cavefish
Match: sp5l (transcription factor Sp5-like [Source:NCBI gene;Acc:103042779])

HSP 1 Score: 159.073 bits (401), Expect = 3.287e-44
Identity = 72/112 (64.29%), Postives = 84/112 (75.00%), Query Frame = 3

Query:  756 RKCQKCTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEG 1085
            R+C++C CPNC     S     G K++HIC+I  C K Y KTSHLKAH+RWH  +RPFVC +  C KSFTRSDELQRH+RTHTGEKRF C  C KRFMRSDHL+KH KTH+G
Sbjct:  236 RRCRRCKCPNCQTSGGSSLEF-GKKRLHICHIPECGKVYKKTSHLKAHLRWHAGERPFVCNWLFCGKSFTRSDELQRHLRTHTGEKRFGCQQCGKRFMRSDHLSKHVKTHQG 346

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BLAST of Sp5 vs. Ensembl Cavefish
Match: sp5a (Sp5 transcription factor [Source:NCBI gene;Acc:103027627])

HSP 1 Score: 155.606 bits (392), Expect = 7.251e-43
Identity = 71/103 (68.93%), Postives = 80/103 (77.67%), Query Frame = 3

Query:  780 PNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            PNC     S  +  G KK H+C+I  C K YGKTSHLKAH+RWH+ +RPFVC +  C KSFTRSDELQRH+RTHTGEKRFVCP C KRFMRSDHLAKH KTH+
Sbjct:  249 PNCQSSSGSGGDEPGKKKQHVCHIPGCGKVYGKTSHLKAHLRWHSGERPFVCNWLFCGKSFTRSDELQRHLRTHTGEKRFVCPDCCKRFMRSDHLAKHVKTHQ 351

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BLAST of Sp5 vs. Ensembl Sea Lamprey
Match: sp4 (sp4 transcription factor [Source:ZFIN;Acc:ZDB-GENE-031202-3])

HSP 1 Score: 157.147 bits (396), Expect = 5.604e-44
Identity = 68/107 (63.55%), Postives = 79/107 (73.83%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLN-GAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            C CPNC  G   R+    G KK H+C+I  C K YGKTSHL+AH+RWH  +RPFVC +  C K FTRSDELQRH RTHTGEK+F CP C KRFMRSDHL+KH +TH+
Sbjct:  271 CACPNCRDGEGRRSGSEPGKKKQHVCHIPGCSKVYGKTSHLRAHLRWHTGERPFVCGWLFCGKRFTRSDELQRHRRTHTGEKKFPCPHCSKRFMRSDHLSKHIRTHQ 377

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BLAST of Sp5 vs. Ensembl Sea Lamprey
Match: sp4 (sp4 transcription factor [Source:ZFIN;Acc:ZDB-GENE-031202-3])

HSP 1 Score: 157.918 bits (398), Expect = 1.570e-43
Identity = 68/107 (63.55%), Postives = 79/107 (73.83%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLN-GAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            C CPNC  G   R+    G KK H+C+I  C K YGKTSHL+AH+RWH  +RPFVC +  C K FTRSDELQRH RTHTGEK+F CP C KRFMRSDHL+KH +TH+
Sbjct:  315 CACPNCRDGEGRRSGSEPGKKKQHVCHIPGCSKVYGKTSHLRAHLRWHTGERPFVCGWLFCGKRFTRSDELQRHRRTHTGEKKFPCPHCSKRFMRSDHLSKHIRTHQ 421

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BLAST of Sp5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008916.1 (pep scaffold:Pmarinus_7.0:GL476670:39536:47416:-1 gene:ENSPMAG00000008063.1 transcript:ENSPMAT00000008916.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 158.688 bits (400), Expect = 1.073e-42
Identity = 68/105 (64.76%), Postives = 77/105 (73.33%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTH 1079
            C CPNC           G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPFVCT+  C K FTRSDELQRH RTHTGEK+F+C  C KRFMRSDHL+KH KTH
Sbjct:  564 CMCPNCRDSEGKVTGEAGQKKQHICHITGCSKVYGKTSHLRAHLRWHSGERPFVCTWLHCGKRFTRSDELQRHKRTHTGEKKFICSECSKRFMRSDHLSKHMKTH 668

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BLAST of Sp5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000432.1 (pep scaffold:Pmarinus_7.0:GL477956:42837:43256:-1 gene:ENSPMAG00000000389.1 transcript:ENSPMAT00000000432.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 135.961 bits (341), Expect = 5.408e-39
Identity = 61/116 (52.59%), Postives = 81/116 (69.83%), Query Frame = 3

Query:  738 IPQLGQRKCQKCTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTH 1079
            + Q   R+C +C CPNC    N+    + +++ H C++  C K YGKTSHL+AH+RWH  +RP+ C + LC++ FTRSDEL+RH RTHTGE+RF C  C KRF RSDHL KH +TH
Sbjct:    5 LAQPSTRRCHRCRCPNCQTASNTATISSSSRRQHTCHVSGCGKAYGKTSHLRAHLRWHAGERPYACAWPLCARRFTRSDELRRHARTHTGERRFACVACGKRFARSDHLGKHARTH 120

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BLAST of Sp5 vs. Ensembl Sea Lamprey
Match: KLF10 (Kruppel like factor 10 [Source:HGNC Symbol;Acc:HGNC:11810])

HSP 1 Score: 119.398 bits (298), Expect = 8.823e-30
Identity = 49/86 (56.98%), Postives = 62/86 (72.09%), Query Frame = 3

Query:  828 KKVHIC--YICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTH 1079
            ++ H+C    C KTY K+SHLKAH+R H  ++PF C++  C + F RSDEL RH R HTGEKRF CP C +RF+RSDHLAKH + H
Sbjct:  343 RRTHVCSHQGCGKTYFKSSHLKAHLRTHTGEKPFRCSWEGCERRFARSDELSRHRRAHTGEKRFACPACARRFLRSDHLAKHARRH 428

HSP 2 Score: 61.6178 bits (148), Expect = 1.525e-10
Identity = 27/59 (45.76%), Postives = 39/59 (66.10%), Query Frame = 3

Query:  915 RPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPL--CFKRFMRSDHLAKHKKTHEG 1085
            R  VC++  C K++ +S  L+ H+RTHTGEK F C    C +RF RSD L++H++ H G
Sbjct:  344 RTHVCSHQGCGKTYFKSSHLKAHLRTHTGEKPFRCSWEGCERRFARSDELSRHRRAHTG 402

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BLAST of Sp5 vs. Ensembl Yeast
Match: AZF1 (Zinc-finger transcription factor; involved in diauxic shift; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000005639])

HSP 1 Score: 88.9669 bits (219), Expect = 2.857e-19
Identity = 40/92 (43.48%), Postives = 55/92 (59.78%), Query Frame = 3

Query:  825 AKKVHICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIV 1100
            A K H C  CH+ + + +HL+ H+R H   +PFVC Y  C K FT+   L+ H R HTGEK + C +C K+F R  +LA H  TH+ + P V
Sbjct:  589 AVKKHECPYCHRLFSQATHLEVHVRSHIGYKPFVCDY--CGKRFTQGGNLRTHERLHTGEKPYSCDICDKKFSRKGNLAAHLVTHQKLKPFV 678

HSP 2 Score: 46.2098 bits (108), Expect = 9.689e-6
Identity = 23/67 (34.33%), Postives = 37/67 (55.22%), Query Frame = 3

Query:  792 QGLNSRAN--LNGAKKVHICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHM 986
            QG N R +  L+  +K + C IC K + +  +L AH+  H   +PFVC    C+K+FT+   ++ H 
Sbjct:  632 QGGNLRTHERLHTGEKPYSCDICDKKFSRKGNLAAHLVTHQKLKPFVCKLENCNKTFTQLGNMKAHQ 698

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BLAST of Sp5 vs. Ensembl Yeast
Match: CRZ1 (Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium [Source:SGD;Acc:S000004972])

HSP 1 Score: 79.7221 bits (195), Expect = 1.899e-16
Identity = 37/90 (41.11%), Postives = 54/90 (60.00%), Query Frame = 3

Query:  843 CYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCP---------LCFKRFMRSDHLAKHKKTHEG 1085
            C +C K + +  +LK+H+R H N+RPF+C+  +C K+F R  + +RH   HTG+KR+VC           C K+F RSD L +H KT  G
Sbjct:  571 CDVCGKKFTRPYNLKSHLRTHTNERPFICS--ICGKAFARQHDRKRHEDLHTGKKRYVCGGKLKDGKPWGCGKKFARSDALGRHFKTESG 658

HSP 2 Score: 53.1434 bits (126), Expect = 6.133e-8
Identity = 25/62 (40.32%), Postives = 33/62 (53.23%), Query Frame = 3

Query:  921 FVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLG 1106
            F C   +C K FTR   L+ H+RTHT E+ F+C +C K F R     +H+  H G    V G
Sbjct:  569 FAC--DVCGKKFTRPYNLKSHLRTHTNERPFICSICGKAFARQHDRKRHEDLHTGKKRYVCG 628

HSP 3 Score: 47.3654 bits (111), Expect = 3.572e-6
Identity = 25/69 (36.23%), Postives = 33/69 (47.83%), Query Frame = 3

Query:  840 ICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHL-------CSKSFTRSDELQRHMRTHTGEKRFVCPL 1025
            IC IC K + +    K H   H   + +VC   L       C K F RSD L RH +T +G +R + PL
Sbjct:  598 ICSICGKAFARQHDRKRHEDLHTGKKRYVCGGKLKDGKPWGCGKKFARSDALGRHFKTESG-RRCITPL 665

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BLAST of Sp5 vs. Ensembl Yeast
Match: ZAP1 (Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains [Source:SGD;Acc:S000003592])

HSP 1 Score: 74.3294 bits (181), Expect = 1.259e-14
Identity = 42/111 (37.84%), Postives = 59/111 (53.15%), Query Frame = 3

Query:  768 KCTCPNCHQGLNSRANLNGAKKVHI------CYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            +C   +CH+    R  L    KVH       C  C + +     L  H R H+ ++P+ C  H+C+K F  S  L+ H+RTHTGEK   C +C KRF  S +L+KH KTH+
Sbjct:  739 QCLWHDCHRTFPQRQKLIRHLKVHSKYKPYKCKTCKRCFSSEETLVQHTRTHSGEKPYKC--HICNKKFAISSSLKIHIRTHTGEKPLQCKICGKRFNESSNLSKHIKTHQ 847

HSP 2 Score: 65.855 bits (159), Expect = 6.277e-12
Identity = 40/133 (30.08%), Postives = 67/133 (50.38%), Query Frame = 3

Query:  732 ANIPQLGQRKCQKCTCPNCHQGLNSRANLNG-AKKVHIC----------YICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPI 1097
            +NIP + Q +   C    C++  +S   LN   + VH+           + CH+T+ +   L  H++ H+  +P+ C    C + F+  + L +H RTH+GEK + C +C K+F  S  L  H +TH G  P+
Sbjct:  694 SNIPPIKQPEQVICQWDGCNKSFSSAQELNDHLEAVHLTRGKSEYQCLWHDCHRTFPQRQKLIRHLKVHSKYKPYKCK--TCKRCFSSEETLVQHTRTHSGEKPYKCHICNKKFAISSSLKIHIRTHTGEKPL 824

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BLAST of Sp5 vs. Ensembl Yeast
Match: MIG2 (Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced gene repression; under low glucose conditions relocalizes to mitochondrion, where it interacts with Ups1p, antagonizes mitochondrial fission factor Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth in response to glucose depletion; activated in stochastic pulses of nuclear localization in response to low glucose [Source:SGD;Acc:S000003177])

HSP 1 Score: 68.9366 bits (167), Expect = 2.750e-13
Identity = 35/106 (33.02%), Postives = 56/106 (52.83%), Query Frame = 3

Query:  843 CYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLGTVISKDTNSSIKSPKALN 1160
            C  CH+ + +  H K H+R H  ++P  C +  C KSF+RSDEL+RHMRTHTG+ +       +R  ++    +   T  G+  I  G +I +     + +  A+N
Sbjct:   19 CDTCHRGFHRLEHKKRHLRTHTGEKPHHCAFPGCGKSFSRSDELKRHMRTHTGQSQ-------RRLKKASVQKQEFLTVSGIPTIASGVMIHQPIPQVLPANMAIN 117

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BLAST of Sp5 vs. Ensembl Yeast
Match: MSN2 (Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes; relative distribution to nucleus increases upon DNA replication stress [Source:SGD;Acc:S000004640])

HSP 1 Score: 63.929 bits (154), Expect = 2.334e-11
Identity = 29/58 (50.00%), Postives = 44/58 (75.86%), Query Frame = 3

Query:  912 DRPFVCTYHLCSKSFTRSDELQRHMRT-HTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            ++PF C  H+C KSF RS+ L+RH+R+ H+ E+ F C +C K+F RSD+L++H KTH+
Sbjct:  644 EKPFHC--HICPKSFKRSEHLKRHVRSVHSNERPFACHICDKKFSRSDNLSQHIKTHK 699

HSP 2 Score: 62.7734 bits (151), Expect = 5.223e-11
Identity = 26/52 (50.00%), Postives = 39/52 (75.00%), Query Frame = 3

Query:  843 CYICHKTYGKTSHLKAHIR-WHNNDRPFVCTYHLCSKSFTRSDELQRHMRTH 995
            C+IC K++ ++ HLK H+R  H+N+RPF C  H+C K F+RSD L +H++TH
Sbjct:  649 CHICPKSFKRSEHLKRHVRSVHSNERPFAC--HICDKKFSRSDNLSQHIKTH 698

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BLAST of Sp5 vs. Ensembl Nematostella
Match: EDO42885 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S016])

HSP 1 Score: 156.762 bits (395), Expect = 8.413e-44
Identity = 80/149 (53.69%), Postives = 97/149 (65.10%), Query Frame = 3

Query:  702 STQLNLSSNRANIPQLGQRKCQK--CTCPNCHQGLNSRANLNGA----KKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLGTVISKD 1124
             T   L S  + +P+  +R   +  C CPNC    N R + +GA    K  HIC+I  C K YGKTSHLKAH+RWH  +RPFVC +  C K FTRSDELQRH+RTHTGEKRF CP+C KRFMRSDHL+KH KTH  + P    +  S+D
Sbjct:  221 GTAAFLQSTPSLVPRTTRRYTGRATCDCPNCQD--NERMSASGAPFRKKSQHICHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPICSKRFMRSDHLSKHVKTHNNITPKKADSEKSED 367

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BLAST of Sp5 vs. Ensembl Nematostella
Match: EDO42941 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S019])

HSP 1 Score: 145.206 bits (365), Expect = 2.263e-42
Identity = 68/105 (64.76%), Postives = 79/105 (75.24%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTH 1079
            CTCPNC  G    AN    +K H+C++  C K YGKTSHL+AH+RWH+ +RPFVC +  C K FTRSDELQRH RTHTGEKRF CP C K+FMRSDHL+KH KTH
Sbjct:   12 CTCPNCRDGEGRTAN---GRKQHVCHVPGCGKVYGKTSHLRAHLRWHSGERPFVCNWLFCGKRFTRSDELQRHRRTHTGEKRFTCPECNKKFMRSDHLSKHVKTH 113

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BLAST of Sp5 vs. Ensembl Nematostella
Match: EDO42939 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S017])

HSP 1 Score: 134.035 bits (336), Expect = 1.666e-38
Identity = 62/87 (71.26%), Postives = 71/87 (81.61%), Query Frame = 3

Query:  828 KKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            +K H+C+I  C K YGKTSHLKAH+RWH  +RPFVC +  C KSFTRSDELQRH+RTHTGEKRF C  C KRFMRSDHL KH+KTH+
Sbjct:    5 RKQHVCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKSFTRSDELQRHLRTHTGEKRFQCDECGKRFMRSDHLRKHQKTHQ 91

[Close]

BLAST of Sp5 vs. Ensembl Nematostella
Match: EDO32290 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SVG8])

HSP 1 Score: 115.931 bits (289), Expect = 1.026e-31
Identity = 56/85 (65.88%), Postives = 64/85 (75.29%), Query Frame = 3

Query:  831 KVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTH 1079
            K H C    C+K YGK+SHLKAH R H  +RPF+CT+  C K F RSDEL RH RTHTGEKR+VC +C KRFMRSDHL+KH KTH
Sbjct:    3 KKHACTYAGCNKIYGKSSHLKAHTRTHTGERPFMCTWKDCEKRFARSDELARHYRTHTGEKRYVCKICDKRFMRSDHLSKHSKTH 87

HSP 2 Score: 58.5362 bits (140), Expect = 4.015e-11
Identity = 30/61 (49.18%), Postives = 38/61 (62.30%), Query Frame = 3

Query:  924 VCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPL--CFKRFMRSDHLAKHKKTHEGVDPIV 1100
             CTY  C+K + +S  L+ H RTHTGE+ F+C    C KRF RSD LA+H +TH G    V
Sbjct:    6 ACTYAGCNKIYGKSSHLKAHTRTHTGERPFMCTWKDCEKRFARSDELARHYRTHTGEKRYV 66

[Close]

BLAST of Sp5 vs. Ensembl Nematostella
Match: EDO42119 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2C5])

HSP 1 Score: 117.087 bits (292), Expect = 1.123e-31
Identity = 62/117 (52.99%), Postives = 73/117 (62.39%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHL-----CSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIV 1100
            C CPNC  G+NS  +     K H C+   C K YGKTSHLKAH+RWH  +RPFVC +       C KSFTRSDELQRH+RTHT EK   C +C K F RSDHL KH + H+    + 
Sbjct:    4 CACPNCVSGINSNPSPT-HPKYHNCHYPGCGKIYGKTSHLKAHLRWHRGERPFVCNWQTILGSTCGKSFTRSDELQRHLRTHTKEKAHTCHVCGKAFGRSDHLTKHLRIHKAKGQLA 119

[Close]

BLAST of Sp5 vs. Ensembl Medaka
Match: SP3 (Sp3 transcription factor [Source:NCBI gene;Acc:101162255])

HSP 1 Score: 168.703 bits (426), Expect = 1.251e-45
Identity = 76/119 (63.87%), Postives = 87/119 (73.11%), Query Frame = 3

Query:  744 QLGQRKCQ----KCTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            QLGQ   +     CTCPNC      R +  G KK HIC+I  C K YGKTSHL+AH+RWH+ +RPFVC++  C K FTRSDELQRH RTHTGEK+FVCP C KRFMRSDHLAKH KTH+
Sbjct:  482 QLGQEGKRLRRVACTCPNCKDS-GGRGSGTGKKKQHICHIVGCGKVYGKTSHLRAHLRWHSGERPFVCSWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQ 599

[Close]

BLAST of Sp5 vs. Ensembl Medaka
Match: sp5l (transcription factor Sp5 [Source:NCBI gene;Acc:101169109])

HSP 1 Score: 154.836 bits (390), Expect = 1.234e-42
Identity = 70/111 (63.06%), Postives = 83/111 (74.77%), Query Frame = 3

Query:  756 RKCQKCTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            R+C++C CPNC    N      G K++HIC+I  C K Y KTSHLKAH+RWH  +RPF+C +  C KSFTRSDELQRH+RTHTGEKRF C  C KRFMRSDHL+KH KTH+
Sbjct:  235 RRCRRCKCPNCQ--ANGGGLEFGKKRLHICHIPECGKVYKKTSHLKAHLRWHAGERPFICNWLFCGKSFTRSDELQRHLRTHTGEKRFGCQQCGKRFMRSDHLSKHVKTHQ 343

[Close]

BLAST of Sp5 vs. Ensembl Medaka
Match: sp4 (Sp4 transcription factor [Source:NCBI gene;Acc:101167131])

HSP 1 Score: 159.844 bits (403), Expect = 2.471e-42
Identity = 68/106 (64.15%), Postives = 78/106 (73.58%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            C+CPNC  G    +     KK HIC++  C K YGKTSHL+AH+RWH  +RPFVC +  C K FTRSDELQRH RTHTGEKRF CP C KRFMRSDHL+KH KTH+
Sbjct:  564 CSCPNCRDGEGRNSGDPTKKKQHICHMEGCGKVYGKTSHLRAHLRWHTGERPFVCNWIFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHIKTHQ 669

[Close]

BLAST of Sp5 vs. Ensembl Medaka
Match: sp9 (Sp9 transcription factor [Source:NCBI gene;Acc:101162490])

HSP 1 Score: 155.606 bits (392), Expect = 3.164e-42
Identity = 80/153 (52.29%), Postives = 101/153 (66.01%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGA----KKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLGTVISKDTNSSIKSPKALNPDKIYKK---QQNVKKP 1202
            C CPNC +    R    GA    K +H C+I  C K YGKTSHLKAH+RWH  +RPFVC +  C K FTRSDELQRH+RTHTGEKRF CP+C KRFMRSDHL+KH KTH        G+    DT S++++P++ +P+ I +    + +VK P
Sbjct:  284 CDCPNCQEA--ERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHIKTHTAGGGGKKGSDSDTDT-SNLETPRSESPELILEGVNPRISVKDP 433

[Close]

BLAST of Sp5 vs. Ensembl Medaka
Match: sp5a (Sp5 transcription factor [Source:NCBI gene;Acc:101158898])

HSP 1 Score: 152.14 bits (383), Expect = 1.571e-41
Identity = 72/103 (69.90%), Postives = 81/103 (78.64%), Query Frame = 3

Query:  780 PNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHE 1082
            PNC    +S  +  G KK HIC+I  C K YGKTSHLKAH+RWH+ +RPFVC +  C KSFTRSDELQRH+RTHTGEKRFVCP C KRFMRSDHLAKH KTH+
Sbjct:  248 PNCQSSTSS--DEPGKKKQHICHIPGCGKVYGKTSHLKAHLRWHSGERPFVCNWLFCGKSFTRSDELQRHLRTHTGEKRFVCPDCCKRFMRSDHLAKHVKTHQ 348

[Close]

BLAST of Sp5 vs. Planmine SMEST
Match: SMESG000023227.1 (SMESG000023227.1)

HSP 1 Score: 733.021 bits (1891), Expect = 0.000e+0
Identity = 373/373 (100.00%), Postives = 373/373 (100.00%), Query Frame = 3

Query:  150 MNSGPAIINIPTSIHVLPSSPGYFSSKSHHLLSGPISPNIDMNYIPNQTNCSTRFLMNSNDFWQSPEMAALQRTTEKFFVNQSLINNPTNPSAXXXXXXXXXXXXXXXARKPCIELPSLIDSKNQMQLMNSLSSHALEDCVDHFFRRSINCFSSGYNLPNLMACKEFSLDFPSADSVRNSPPNISTQLNLSSNRANIPQLGQRKCQKCTCPNCHQGLNSRANLNGAKKVHICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLGTVISKDTNSSIKSPKALNPDKIYKKQQNVKKPRLGLSVSNSAMNSPDHHHLHGK 1268
            MNSGPAIINIPTSIHVLPSSPGYFSSKSHHLLSGPISPNIDMNYIPNQTNCSTRFLMNSNDFWQSPEMAALQRTTEKFFVNQSLINNPTNPSAIPNPTNCPTTPIRPIARKPCIELPSLIDSKNQMQLMNSLSSHALEDCVDHFFRRSINCFSSGYNLPNLMACKEFSLDFPSADSVRNSPPNISTQLNLSSNRANIPQLGQRKCQKCTCPNCHQGLNSRANLNGAKKVHICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLGTVISKDTNSSIKSPKALNPDKIYKKQQNVKKPRLGLSVSNSAMNSPDHHHLHGK
Sbjct:    1 MNSGPAIINIPTSIHVLPSSPGYFSSKSHHLLSGPISPNIDMNYIPNQTNCSTRFLMNSNDFWQSPEMAALQRTTEKFFVNQSLINNPTNPSAIPNPTNCPTTPIRPIARKPCIELPSLIDSKNQMQLMNSLSSHALEDCVDHFFRRSINCFSSGYNLPNLMACKEFSLDFPSADSVRNSPPNISTQLNLSSNRANIPQLGQRKCQKCTCPNCHQGLNSRANLNGAKKVHICYICHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEGVDPIVLGTVISKDTNSSIKSPKALNPDKIYKKQQNVKKPRLGLSVSNSAMNSPDHHHLHGK 373

[Close]

BLAST of Sp5 vs. Planmine SMEST
Match: SMESG000050160.1 (SMESG000050160.1)

HSP 1 Score: 114.005 bits (284), Expect = 4.896e-29
Identity = 54/105 (51.43%), Postives = 68/105 (64.76%), Query Frame = 3

Query:  771 CTCPNCHQGLNSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTH 1079
            C+CPNC +  N    L      H C+I  C K Y K SHLKAH+RWH  +RPFVC +  C K F RSDELQ+H++ HT EK+  CP C K F+RS+ L+KH +TH
Sbjct:  114 CSCPNCQEMENLSGYL-SKNNSHNCHIAGCGKLYSKPSHLKAHLRWHTGERPFVCNWLFCGKRFNRSDELQKHLKVHTDEKKLNCPKCGKHFVRSEQLSKHIQTH 217

[Close]

BLAST of Sp5 vs. Planmine SMEST
Match: SMESG000025482.1 (SMESG000025482.1)

HSP 1 Score: 106.301 bits (264), Expect = 1.276e-26
Identity = 47/78 (60.26%), Postives = 58/78 (74.36%), Query Frame = 3

Query:  852 CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEG 1085
            C K Y K+SHLKAHIR H  D+P++C +  C + FTRSDEL RH RTHTGEK F C +C ++F RSDHL+KH KTH+ 
Sbjct:  101 CLKAYYKSSHLKAHIRVHTGDKPYICNWENCQRRFTRSDELSRHKRTHTGEKLFSCSICQRKFNRSDHLSKHIKTHKN 178

HSP 2 Score: 60.077 bits (144), Expect = 2.943e-10
Identity = 26/59 (44.07%), Postives = 39/59 (66.10%), Query Frame = 3

Query:  915 RPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPL--CFKRFMRSDHLAKHKKTHEG 1085
            + F C Y  C K++ +S  L+ H+R HTG+K ++C    C +RF RSD L++HK+TH G
Sbjct:   92 KSFKCNYKSCLKAYYKSSHLKAHIRVHTGDKPYICNWENCQRRFTRSDELSRHKRTHTG 150

[Close]

BLAST of Sp5 vs. Planmine SMEST
Match: SMESG000025482.1 (SMESG000025482.1)

HSP 1 Score: 106.686 bits (265), Expect = 1.335e-26
Identity = 47/78 (60.26%), Postives = 58/78 (74.36%), Query Frame = 3

Query:  852 CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRSDHLAKHKKTHEG 1085
            C K Y K+SHLKAHIR H  D+P++C +  C + FTRSDEL RH RTHTGEK F C +C ++F RSDHL+KH KTH+ 
Sbjct:  115 CLKAYYKSSHLKAHIRVHTGDKPYICNWENCQRRFTRSDELSRHKRTHTGEKLFSCSICQRKFNRSDHLSKHIKTHKN 192

HSP 2 Score: 60.077 bits (144), Expect = 3.475e-10
Identity = 26/59 (44.07%), Postives = 39/59 (66.10%), Query Frame = 3

Query:  915 RPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPL--CFKRFMRSDHLAKHKKTHEG 1085
            + F C Y  C K++ +S  L+ H+R HTG+K ++C    C +RF RSD L++HK+TH G
Sbjct:  106 KSFKCNYKSCLKAYYKSSHLKAHIRVHTGDKPYICNWENCQRRFTRSDELSRHKRTHTG 164

[Close]

BLAST of Sp5 vs. Planmine SMEST
Match: SMESG000067314.1 (SMESG000067314.1)

HSP 1 Score: 90.8929 bits (224), Expect = 4.005e-20
Identity = 58/182 (31.87%), Postives = 83/182 (45.60%), Query Frame = 3

Query:  660 FPSADSVRNSPPNISTQLN-LSSNRANIPQ---LGQRKCQKCTCPNC---------HQGL-----NSRANLNGAKKVHICYI--CHKTYGKTSHLKAHIRWHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEK-----------------------RFVCPLCFKRFMRSDHLAKHKKT 1076
            + +  S+ N P  + T +N    +  N P+    G      C CPNC         HQ L     N+  +L     +H C++  C K Y K+SHLKAH+RWH  +RPFVC +  C K F RSDELQ+H+R H   K                       +  C  C++ + ++D L KH K+
Sbjct:  121 YSNVSSLLNFPTMLETSINSWPLSNINYPRSSTRGYSSVGNCECPNCQETPFIENTHQKLLPTIMNASESLGQYPILHNCHVPGCGKVYKKSSHLKAHLRWHTGERPFVCNWLFCGKRFARSDELQQHIRLHQETKDNTNSTALSPKKELKTVDKDLGIKLTCEFCYRSYSKNDLLLKHIKS 302

The following BLAST results are available for this feature:

BLAST of Sp5 vs. Ensembl Human
BLAST of Sp5 vs. Ensembl Celegans
BLAST of Sp5 vs. Ensembl Fly
BLAST of Sp5 vs. Ensembl Zebrafish
BLAST of Sp5 vs. Ensembl Xenopus
BLAST of Sp5 vs. Ensembl Mouse
BLAST of Sp5 vs. UniProt/SwissProt
BLAST of Sp5 vs. TrEMBL
BLAST of Sp5 vs. Ensembl Cavefish
BLAST of Sp5 vs. Ensembl Sea Lamprey
BLAST of Sp5 vs. Ensembl Yeast
BLAST of Sp5 vs. Ensembl Nematostella
BLAST of Sp5 vs. Ensembl Medaka
BLAST of Sp5 vs. Planmine SMEST

BLAST of Sp5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SP3	5.665e-46	69.81	Sp3 transcription factor [Source:HGNC Symbol;Acc:H...	[more]
SP3	7.406e-46	69.81	Sp3 transcription factor [Source:HGNC Symbol;Acc:H...	[more]
SP3	7.490e-46	69.81	Sp3 transcription factor [Source:HGNC Symbol;Acc:H...	[more]
SP3	7.946e-46	69.81	Sp3 transcription factor [Source:HGNC Symbol;Acc:H...	[more]
SP3	1.171e-45	69.81	Sp3 transcription factor [Source:HGNC Symbol;Acc:H...	[more]

BLAST of Sp5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sptf-3	1.039e-39	52.27	SP (Specificity Protein) Transcription Factor [So...	[more]
sptf-2	1.798e-30	50.88	SP (Specificity Protein) Transcription Factor [So...	[more]
klf-3	7.596e-23	51.02	Kruppel-Like Factor (Zinc finger protein) [Source...	[more]
klf-3	7.805e-23	51.02	Kruppel-Like Factor (Zinc finger protein) [Source...	[more]
klf-2	9.111e-20	51.16	Kruppel-Like Factor (Zinc finger protein) [Source...	[more]

BLAST of Sp5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Spps	1.818e-40	55.88	gene:FBgn0039169 transcript:FBtr0084565	[more]
Sp1	2.237e-40	63.39	gene:FBgn0020378 transcript:FBtr0071381	[more]
Spps	2.308e-40	55.88	gene:FBgn0039169 transcript:FBtr0330382	[more]
Sp1	5.040e-40	63.39	gene:FBgn0020378 transcript:FBtr0273354	[more]
Sp1	5.139e-40	63.39	gene:FBgn0020378 transcript:FBtr0331335	[more]

BLAST of Sp5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sp3a	7.485e-46	67.92	sp3a transcription factor [Source:ZFIN;Acc:ZDB-GEN...	[more]
sp3a	7.619e-46	67.92	sp3a transcription factor [Source:ZFIN;Acc:ZDB-GEN...	[more]
sp3b	2.460e-45	66.98	Sp3b transcription factor [Source:ZFIN;Acc:ZDB-GEN...	[more]
sp3b	2.852e-45	66.98	Sp3b transcription factor [Source:ZFIN;Acc:ZDB-GEN...	[more]
sp3b	1.266e-44	66.98	Sp3b transcription factor [Source:ZFIN;Acc:ZDB-GEN...	[more]

BLAST of Sp5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
zp3.2	2.522e-46	70.75	zona pellucida glycoprotein 3 (sperm receptor), ge...	[more]
zp3.2	3.139e-46	70.75	zona pellucida glycoprotein 3 (sperm receptor), ge...	[more]
nr2e3	5.699e-46	55.10	nuclear receptor subfamily 2 group E member 3 [Sou...	[more]
nr2e3	1.070e-45	55.10	nuclear receptor subfamily 2 group E member 3 [Sou...	[more]
fxr1	1.702e-44	59.84	FMR1 autosomal homolog 1 [Source:Xenbase;Acc:XB-GE...	[more]

BLAST of Sp5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Sp3	6.807e-46	68.87	trans-acting transcription factor 3 [Source:MGI Sy...	[more]
Sp3	1.037e-45	68.87	trans-acting transcription factor 3 [Source:MGI Sy...	[more]
Sp4	1.251e-44	57.48	trans-acting transcription factor 4 [Source:MGI Sy...	[more]
Sp4	8.417e-44	58.27	trans-acting transcription factor 4 [Source:MGI Sy...	[more]
Sp1	5.659e-43	50.00	trans-acting transcription factor 1 [Source:MGI Sy...	[more]

BLAST of Sp5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q90WR8\|SP3_CHICK	3.484e-45	69.81	Transcription factor Sp3 OS=Gallus gallus OX=9031 ...	[more]
sp\|Q02447\|SP3_HUMAN	5.625e-45	69.81	Transcription factor Sp3 OS=Homo sapiens OX=9606 G...	[more]
sp\|O70494\|SP3_MOUSE	7.257e-45	68.87	Transcription factor Sp3 OS=Mus musculus OX=10090 ...	[more]
sp\|Q02446\|SP4_HUMAN	1.066e-44	59.06	Transcription factor Sp4 OS=Homo sapiens OX=9606 G...	[more]
sp\|Q62445\|SP4_MOUSE	9.924e-44	57.48	Transcription factor Sp4 OS=Mus musculus OX=10090 ...	[more]

BLAST of Sp5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0A7NZ65	0.000e+0	100.00	Sp5 (Fragment) OS=Schmidtea mediterranea OX=79327 ...	[more]
A0A224AB84	0.000e+0	80.37	Sp5 OS=Dugesia japonica OX=6161 PE=2 SV=1	[more]
A0A448XCY8	3.867e-49	64.96	Uncharacterized protein OS=Protopolystoma xenopodi...	[more]
A0A5E4Q5Z4	8.204e-46	50.86	Uncharacterized protein OS=Leptidea sinapis OX=189...	[more]
A0A087VHY4	6.926e-45	71.70	Transcription factor Sp3 (Fragment) OS=Balearica r...	[more]

BLAST of Sp5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sp3a	3.327e-46	60.16	Sp3 transcription factor [Source:NCBI gene;Acc:103...	[more]
sp3a	4.844e-46	60.16	Sp3 transcription factor [Source:NCBI gene;Acc:103...	[more]
sp3b	3.605e-45	67.62	transcription factor Sp3-like [Source:NCBI gene;Ac...	[more]
sp5l	3.287e-44	64.29	transcription factor Sp5-like [Source:NCBI gene;Ac...	[more]
sp5a	7.251e-43	68.93	Sp5 transcription factor [Source:NCBI gene;Acc:103...	[more]

BLAST of Sp5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sp4	5.604e-44	63.55	sp4 transcription factor [Source:ZFIN;Acc:ZDB-GENE...	[more]
sp4	1.570e-43	63.55	sp4 transcription factor [Source:ZFIN;Acc:ZDB-GENE...	[more]
ENSPMAT00000008916.1	1.073e-42	64.76	pep scaffold:Pmarinus_7.0:GL476670:39536:47416:-1 ...	[more]
ENSPMAT00000000432.1	5.408e-39	52.59	pep scaffold:Pmarinus_7.0:GL477956:42837:43256:-1 ...	[more]
KLF10	8.823e-30	56.98	Kruppel like factor 10 [Source:HGNC Symbol;Acc:HGN...	[more]

BLAST of Sp5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5


Match Name	E-value	Identity	Description
AZF1	2.857e-19	43.48	Zinc-finger transcription factor; involved in diau...	[more]
CRZ1	1.899e-16	41.11	Transcription factor, activates transcription of s...	[more]
ZAP1	1.259e-14	37.84	Zinc-regulated transcription factor; binds to zinc...	[more]
MIG2	2.750e-13	33.02	Zinc finger transcriptional repressor; cooperates ...	[more]
MSN2	2.334e-11	50.00	Stress-responsive transcriptional activator; activ...	[more]

BLAST of Sp5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO42885	8.413e-44	53.69	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42941	2.263e-42	64.76	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42939	1.666e-38	71.26	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO32290	1.026e-31	65.88	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO42119	1.123e-31	52.99	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Sp5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SP3	1.251e-45	63.87	Sp3 transcription factor [Source:NCBI gene;Acc:101...	[more]
sp5l	1.234e-42	63.06	transcription factor Sp5 [Source:NCBI gene;Acc:101...	[more]
sp4	2.471e-42	64.15	Sp4 transcription factor [Source:NCBI gene;Acc:101...	[more]
sp9	3.164e-42	52.29	Sp9 transcription factor [Source:NCBI gene;Acc:101...	[more]
sp5a	1.571e-41	69.90	Sp5 transcription factor [Source:NCBI gene;Acc:101...	[more]

BLAST of Sp5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000023227.1	0.000e+0	100.00	SMESG000023227.1	[more]
SMESG000050160.1	4.896e-29	51.43	SMESG000050160.1	[more]
SMESG000025482.1	1.276e-26	60.26	SMESG000025482.1	[more]
SMESG000025482.1	1.335e-26	60.26	SMESG000025482.1	[more]
SMESG000067314.1	4.005e-20	31.87	SMESG000067314.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Cross References

External references for this transcript


Database	Accession
SmedGD GBrowse	SMED30033839
GenBank	AIZ98420.1

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30033839 ID=SMED30033839|Name=Sp5|organism=Schmidtea mediterranea sexual|type=transcript|length=1336bp
TGTTTTTTATTTTCAAGTATTAATATATTCAAAATTCAAGTCCAATAAGA
ATTTATTATTATTATTTTTTTATTATTGTATTTTTTAAACCAAAACCCAT
TCAAAAGAAATTGATTGATTTTCGAAATTGAAATTATCCAAAAACAAAAA
TGAATTCCGGACCAGCTATAATAAACATTCCAACGTCGATTCACGTTTTA
CCATCGTCACCGGGATATTTCTCCAGCAAATCCCATCATTTACTTTCTGG
TCCAATTTCTCCAAATATCGATATGAATTACATCCCAAATCAGACTAATT
GCTCGACGAGATTCTTAATGAATTCCAACGATTTTTGGCAGAGTCCAGAA
ATGGCGGCCCTTCAAAGAACGACTGAAAAGTTTTTCGTCAATCAAAGTCT
TATAAATAACCCAACCAATCCATCGGCTATTCCTAATCCCACTAACTGTC
CGACCACTCCTATTAGACCTATCGCTAGAAAGCCTTGCATTGAACTTCCT
TCATTAATAGATTCTAAAAATCAAATGCAATTGATGAATTCTTTGTCTTC
CCATGCTTTAGAAGATTGCGTTGACCATTTCTTTCGTAGATCTATTAACT
GTTTTTCGTCTGGTTACAATCTTCCAAATTTAATGGCTTGCAAAGAGTTT
TCTTTAGACTTTCCATCAGCCGACAGCGTTAGAAACAGCCCACCAAACAT
ATCGACACAATTGAACTTATCATCAAACCGAGCAAACATTCCACAATTGG
GTCAGAGAAAATGTCAGAAGTGTACTTGTCCGAACTGTCATCAAGGTCTC
AATTCTCGAGCCAATCTCAATGGAGCGAAAAAAGTTCACATTTGTTACAT
TTGTCACAAAACATATGGAAAAACCAGTCATTTAAAAGCCCATATCAGGT
GGCACAACAACGATCGGCCGTTTGTTTGTACGTATCACTTATGTTCGAAA
TCTTTCACCAGATCAGATGAATTACAGAGACACATGAGAACTCACACAGG
AGAAAAGCGATTCGTTTGTCCTTTGTGTTTTAAAAGATTCATGCGCAGTG
ATCATCTAGCAAAACACAAGAAAACACACGAAGGCGTCGATCCGATAGTT
CTCGGTACAGTCATATCGAAAGACACAAATTCTTCCATTAAATCACCAAA
AGCTCTGAATCCAGACAAAATTTATAAAAAGCAGCAGAATGTAAAGAAAC
CGAGACTTGGTCTTTCTGTCTCAAACAGCGCTATGAATTCTCCAGATCAT
CACCATCTCCATGGGAAAGAAAACATTATCATTGAAGAAGATGTTGACAT
AGAATCTATTTGATTTTCATTTTGTGTAAAATCGTG

protein sequence of SMED30033839-orf-1

>SMED30033839-orf-1 ID=SMED30033839-orf-1|Name=SMED30033839-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=388bp
MNSGPAIINIPTSIHVLPSSPGYFSSKSHHLLSGPISPNIDMNYIPNQTN
CSTRFLMNSNDFWQSPEMAALQRTTEKFFVNQSLINNPTNPSAIPNPTNC
PTTPIRPIARKPCIELPSLIDSKNQMQLMNSLSSHALEDCVDHFFRRSIN
CFSSGYNLPNLMACKEFSLDFPSADSVRNSPPNISTQLNLSSNRANIPQL
GQRKCQKCTCPNCHQGLNSRANLNGAKKVHICYICHKTYGKTSHLKAHIR
WHNNDRPFVCTYHLCSKSFTRSDELQRHMRTHTGEKRFVCPLCFKRFMRS
DHLAKHKKTHEGVDPIVLGTVISKDTNSSIKSPKALNPDKIYKKQQNVKK
PRLGLSVSNSAMNSPDHHHLHGKENIIIEEDVDIESI*

Aliases

The feature 'Sp5' has the following synonyms


Synonym
sp5*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000028	Category 2 cell
PLANA:0000034	epidermis
PLANA:0000035	Category 3 cell
PLANA:0000101	muscle cell
PLANA:0000142	posterior region of the whole animal
PLANA:0000224	cement gland
PLANA:0000421	tail
PLANA:0000429	neoblast
PLANA:0002089	reproductive organ
PLANA:0002109	X1 cell
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR036236	Znf_C2H2_sf
IPR015880	Znf_C2H2-like
IPR007087	Znf_C2H2
IPR013087	Znf_C2H2_type

Vocabulary: **molecular function**
Term	Definition
GO:0046872	metal ion binding
GO:0003676	nucleic acid binding
GO:0043565	sequence-specific DNA binding
GO:0000977	RNA polymerase II regulatory region sequence-specific DNA binding
GO:0000981	RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003677	DNA binding

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **biological process**
Term	Definition
GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0006357	regulation of transcription from RNA polymerase II promoter
GO:0036342	post-anal tail morphogenesis
GO:0060349	bone morphogenesis
GO:0071407	cellular response to organic cyclic compound

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013087	Zinc finger C2H2-type	SMART	SM00355	c2h2final6	coord: 288..310 e-value: 0.028 score: 23.6 coord: 230..252 e-value: 30.0 score: 9.1 coord: 258..282 e-value: 0.0041 score: 26.4
IPR013087	Zinc finger C2H2-type	PFAM	PF00096	zf-C2H2	coord: 258..282 e-value: 9.2E-6 score: 25.8 coord: 288..310 e-value: 0.0022 score: 18.4
IPR013087	Zinc finger C2H2-type	PROSITE	PS00028	ZINC_FINGER_C2H2_1	coord: 290..310
IPR013087	Zinc finger C2H2-type	PROSITE	PS00028	ZINC_FINGER_C2H2_1	coord: 232..252
IPR013087	Zinc finger C2H2-type	PROSITE	PS00028	ZINC_FINGER_C2H2_1	coord: 260..282
IPR013087	Zinc finger C2H2-type	PROSITE	PS50157	ZINC_FINGER_C2H2_2	coord: 288..315 score: 13.09
IPR013087	Zinc finger C2H2-type	PROSITE	PS50157	ZINC_FINGER_C2H2_2	coord: 230..257 score: 12.03
IPR013087	Zinc finger C2H2-type	PROSITE	PS50157	ZINC_FINGER_C2H2_2	coord: 258..287 score: 15.521
None	No IPR available	GENE3D	G3DSA:3.30.160.60		coord: 285..315 e-value: 8.7E-10 score: 40.2
None	No IPR available	GENE3D	G3DSA:3.30.160.60		coord: 222..259 e-value: 5.5E-6 score: 28.3
None	No IPR available	GENE3D	G3DSA:3.30.160.60		coord: 260..284 e-value: 7.4E-15 score: 56.5
None	No IPR available	PANTHER	PTHR23235	TRANSCRIPTION FACTOR SP3	coord: 107..346
None	No IPR available	PANTHER	PTHR23235:SF15	SP1, ISOFORM F-RELATED	coord: 107..346
IPR036236	Zinc finger C2H2 superfamily	SUPERFAMILY	SSF57667	beta-beta-alpha zinc fingers	coord: 239..297