SMED30033819
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30033819 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Homology
BLAST of SMED30033819 vs. Ensembl Human
Match: C7orf50 (chromosome 7 open reading frame 50 [Source:HGNC Symbol;Acc:HGNC:22421]) HSP 1 Score: 51.6026 bits (122), Expect = 5.686e-8 Identity = 26/88 (29.55%), Postives = 44/88 (50.00%), Query Frame = 1 Query: 184 DYLLSYEDKS-NWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSI---INDDCSGKTSKNRASKIASILN 435 DYL + K NW+F K Q LL +MY DK F + Y++ LQG ++ ++K ++ ++++ S RA +I +L Sbjct: 72 DYLCRWAQKHKNWRFQKTRQTWLLLHMYDSDKVPDEHFSTLLAYLEGLQGRARELTVQKAEALMRELDEEGSDPPLPGRAQRIRQVLQ 159
BLAST of SMED30033819 vs. Ensembl Human
Match: C7orf50 (chromosome 7 open reading frame 50 [Source:HGNC Symbol;Acc:HGNC:22421]) HSP 1 Score: 51.6026 bits (122), Expect = 7.329e-8 Identity = 26/88 (29.55%), Postives = 44/88 (50.00%), Query Frame = 1 Query: 184 DYLLSYEDKS-NWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSI---INDDCSGKTSKNRASKIASILN 435 DYL + K NW+F K Q LL +MY DK F + Y++ LQG ++ ++K ++ ++++ S RA +I +L Sbjct: 89 DYLCRWAQKHKNWRFQKTRQTWLLLHMYDSDKVPDEHFSTLLAYLEGLQGRARELTVQKAEALMRELDEEGSDPPLPGRAQRIRQVLQ 176
BLAST of SMED30033819 vs. Ensembl Human
Match: C7orf50 (chromosome 7 open reading frame 50 [Source:HGNC Symbol;Acc:HGNC:22421]) HSP 1 Score: 51.6026 bits (122), Expect = 8.391e-8 Identity = 26/88 (29.55%), Postives = 44/88 (50.00%), Query Frame = 1 Query: 184 DYLLSYEDKS-NWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSI---INDDCSGKTSKNRASKIASILN 435 DYL + K NW+F K Q LL +MY DK F + Y++ LQG ++ ++K ++ ++++ S RA +I +L Sbjct: 104 DYLCRWAQKHKNWRFQKTRQTWLLLHMYDSDKVPDEHFSTLLAYLEGLQGRARELTVQKAEALMRELDEEGSDPPLPGRAQRIRQVLQ 191
BLAST of SMED30033819 vs. Ensembl Human
Match: C7orf50 (chromosome 7 open reading frame 50 [Source:HGNC Symbol;Acc:HGNC:22421]) HSP 1 Score: 51.2174 bits (121), Expect = 9.665e-8 Identity = 26/88 (29.55%), Postives = 44/88 (50.00%), Query Frame = 1 Query: 184 DYLLSYEDKS-NWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSI---INDDCSGKTSKNRASKIASILN 435 DYL + K NW+F K Q LL +MY DK F + Y++ LQG ++ ++K ++ ++++ S RA +I +L Sbjct: 104 DYLCRWAQKHKNWRFQKTRQTWLLLHMYDSDKVPDEHFSTLLAYLEGLQGRARELTVQKAEALMRELDEEGSDPPLPGRAQRIRQVLQ 191
BLAST of SMED30033819 vs. Ensembl Human
Match: C7orf50 (chromosome 7 open reading frame 50 [Source:HGNC Symbol;Acc:HGNC:22421]) HSP 1 Score: 51.2174 bits (121), Expect = 9.665e-8 Identity = 26/88 (29.55%), Postives = 44/88 (50.00%), Query Frame = 1 Query: 184 DYLLSYEDKS-NWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSI---INDDCSGKTSKNRASKIASILN 435 DYL + K NW+F K Q LL +MY DK F + Y++ LQG ++ ++K ++ ++++ S RA +I +L Sbjct: 104 DYLCRWAQKHKNWRFQKTRQTWLLLHMYDSDKVPDEHFSTLLAYLEGLQGRARELTVQKAEALMRELDEEGSDPPLPGRAQRIRQVLQ 191
BLAST of SMED30033819 vs. Ensembl Zebrafish
Match: zmp:0000000624 (zmp:0000000624 [Source:ZFIN;Acc:ZDB-GENE-130530-627]) HSP 1 Score: 49.6766 bits (117), Expect = 3.544e-7 Identity = 29/92 (31.52%), Postives = 45/92 (48.91%), Query Frame = 1 Query: 184 DYLLSY-EDKSNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIINDDCSGKTS--------KNRASKIASIL 432 +YL + E + WKF K Q LLQNMY K S F ++ Y+ L+G+++ L+K I+ D G+ + RA +I +L Sbjct: 167 EYLTCWSERREEWKFQKTRQTWLLQNMYDSVKVPDSHFDVLLSYLDGLRGSARDTTLQKAEVILRWDGQGEKEETQDAEKKQQRAKQIVQLL 258
BLAST of SMED30033819 vs. Ensembl Xenopus
Match: rap1gap (RAP1 GTPase activating protein [Source:Xenbase;Acc:XB-GENE-996878]) HSP 1 Score: 48.9062 bits (115), Expect = 6.556e-7 Identity = 29/83 (34.94%), Postives = 42/83 (50.60%), Query Frame = 1 Query: 187 YLLSYEDK-SNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIIND-DCSGKTSKNRASKIASI 429 YL S+ K WKF K Q LL +MY +K FKI+ YI LQG ++ +EK +++ D S ++ K+A I Sbjct: 122 YLKSWSKKHEEWKFQKTRQTWLLLHMYDPEKVPDKYFKILLNYIAGLQGRARDTTVEKAEALMKQYDSSETQEEDHVPKLARI 204
BLAST of SMED30033819 vs. Ensembl Xenopus
Match: rap1gap (RAP1 GTPase activating protein [Source:Xenbase;Acc:XB-GENE-996878]) HSP 1 Score: 48.9062 bits (115), Expect = 7.474e-7 Identity = 29/83 (34.94%), Postives = 42/83 (50.60%), Query Frame = 1 Query: 187 YLLSYEDK-SNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIIND-DCSGKTSKNRASKIASI 429 YL S+ K WKF K Q LL +MY +K FKI+ YI LQG ++ +EK +++ D S ++ K+A I Sbjct: 133 YLKSWSKKHEEWKFQKTRQTWLLLHMYDPEKVPDKYFKILLNYIAGLQGRARDTTVEKAEALMKQYDSSETQEEDHVPKLARI 215
BLAST of SMED30033819 vs. UniProt/SwissProt
Match: sp|Q9BRJ6|CG050_HUMAN (Uncharacterized protein C7orf50 OS=Homo sapiens OX=9606 GN=C7orf50 PE=1 SV=1) HSP 1 Score: 51.2174 bits (121), Expect = 4.641e-7 Identity = 26/88 (29.55%), Postives = 44/88 (50.00%), Query Frame = 1 Query: 184 DYLLSYEDKS-NWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSI---INDDCSGKTSKNRASKIASILN 435 DYL + K NW+F K Q LL +MY DK F + Y++ LQG ++ ++K ++ ++++ S RA +I +L Sbjct: 104 DYLCRWAQKHKNWRFQKTRQTWLLLHMYDSDKVPDEHFSTLLAYLEGLQGRARELTVQKAEALMRELDEEGSDPPLPGRAQRIRQVLQ 191
BLAST of SMED30033819 vs. TrEMBL
Match: G4YM97 (Uncharacterized protein OS=Phytophthora sojae (strain P6497) OX=1094619 GN=PHYSODRAFT_248463 PE=4 SV=1) HSP 1 Score: 60.8474 bits (146), Expect = 4.083e-8 Identity = 33/123 (26.83%), Postives = 64/123 (52.03%), Query Frame = 1 Query: 151 NESSDIIAKIRDYLLSYE--------DKSNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIINDDCSGKTSKNRAS-KIASILNSTLQNS*YNIVIEFYQIFA 492 + S ++I + + YL ++ D+ WKF K+ Q+ +LQ MY D ++F IV +Y+ +QGA++TRV+E +I+ K ++ A+ + +L+ L Y ++ ++ A Sbjct: 62 DRSPEMIQQAKYYLEQWQKRDETKADDELPWKFKKIKQQWILQWMYEADVVPKAMFAIVLEYLNGIQGAARTRVMESAQEVIDAGVPSKDEEDAAADEKEKMLSIKLARRRYKRALQVAELLA 184
BLAST of SMED30033819 vs. TrEMBL
Match: A0A0C9XUB2 (Unplaced genomic scaffold K443scaffold_7, whole genome shotgun sequence OS=Laccaria amethystina LaAM-08-1 OX=1095629 GN=K443DRAFT_85301 PE=4 SV=1) HSP 1 Score: 58.9214 bits (141), Expect = 3.280e-7 Identity = 32/105 (30.48%), Postives = 55/105 (52.38%), Query Frame = 1 Query: 121 HKNKTSVSDINESSDIIAKIRDYL----LSYEDKSNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIINDDCSGKTSKNRASKIA 423 HKN+T ++D E + + + R L L + S WKFNK Q +++N++ + +V KY+ +QG S+T+++E C SI+ D ++N K A Sbjct: 78 HKNRTGLADPREDTVLSNQARKGLEYAFLQFHRPSKWKFNKARQNWIIRNVWSAENVPEMYLPLVIKYLTNVQGGSRTKLIETCQSIL-DKPHKPEAENPEEKPA 181
BLAST of SMED30033819 vs. TrEMBL
Match: S4RKQ7 (Zmp:0000000624 OS=Petromyzon marinus OX=7757 PE=4 SV=1) HSP 1 Score: 57.3806 bits (137), Expect = 3.361e-7 Identity = 28/65 (43.08%), Postives = 40/65 (61.54%), Query Frame = 1 Query: 184 DYLLSYE-DKSNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIIN 375 +YL S+ DK WKF K+ Q LLQ+MY +K S F+I+ KY+ L+G +K +EK SI+ Sbjct: 54 EYLKSWATDKEQWKFQKLRQTWLLQHMYDAEKVPDSYFRILLKYLAGLKGQAKDTTIEKAESIMR 118
BLAST of SMED30033819 vs. TrEMBL
Match: A0A2D4C909 (Uncharacterized protein OS=Pythium insidiosum OX=114742 GN=PINS_010900 PE=4 SV=1) HSP 1 Score: 58.9214 bits (141), Expect = 3.813e-7 Identity = 30/99 (30.30%), Postives = 53/99 (53.54%), Query Frame = 1 Query: 196 SYEDKSNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIINDDCSGKTSKNRASKIASILNSTLQNS*YNIVIEFYQIFA 492 + + + WKF KV Q +L+ M+ D S+F +V +Y++ +QGA++ RVL+ + II++ KT + A +AS L Y ++ Q+ A Sbjct: 124 TPDGEEPWKFKKVKQLWILKWMFEADVIPKSMFALVLEYLEGMQGAARQRVLDDAHKIIDEGVPDKTDDDAAEDLASRLTRRR----YKRALQIAQVLA 218
BLAST of SMED30033819 vs. TrEMBL
Match: A0A067TM27 (Uncharacterized protein OS=Galerina marginata (strain CBS 339.88) OX=685588 GN=GALMADRAFT_235499 PE=4 SV=1) HSP 1 Score: 58.151 bits (139), Expect = 8.324e-7 Identity = 28/89 (31.46%), Postives = 50/89 (56.18%), Query Frame = 1 Query: 121 HKNKTSVSDINESSDIIAKIR---DYLLSYEDK-SNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIIN 375 HKN+T +D E +++ + R +Y + ++ S WKFNK Q L++N++ + F + KY+ A+QG S+ ++ + C S IN Sbjct: 73 HKNRTGFADPREDTNLNNQSRKALEYAFTQMNRPSKWKFNKARQNWLIRNIWAPEALPEVYFPLAVKYLAAVQGGSREKLTQTCQSYIN 161
BLAST of SMED30033819 vs. Ensembl Sea Lamprey
Match: zmp:0000000624 (zmp:0000000624 [Source:ZFIN;Acc:ZDB-GENE-130530-627]) HSP 1 Score: 57.3806 bits (137), Expect = 2.582e-11 Identity = 28/65 (43.08%), Postives = 40/65 (61.54%), Query Frame = 1 Query: 184 DYLLSYE-DKSNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIIN 375 +YL S+ DK WKF K+ Q LLQ+MY +K S F+I+ KY+ L+G +K +EK SI+ Sbjct: 54 EYLKSWATDKEQWKFQKLRQTWLLQHMYDAEKVPDSYFRILLKYLAGLKGQAKDTTIEKAESIMR 118
BLAST of SMED30033819 vs. Ensembl Medaka
Match: zmp:0000000624 (chromosome 19 C7orf50 homolog [Source:NCBI gene;Acc:101164188]) HSP 1 Score: 47.3654 bits (111), Expect = 2.030e-6 Identity = 25/86 (29.07%), Postives = 48/86 (55.81%), Query Frame = 1 Query: 202 EDKSNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIINDDCSGKTSK--------NRASKIASILN 435 E++S WKF K Q LLQ+M+ +K F ++ +Y++ L+G ++ +EK S++ + SG+ + +RA +I +L+ Sbjct: 158 ENRSVWKFQKTRQTWLLQHMFDSEKIPDEKFSLLLQYLEGLRGMARDTTVEKAMSMVEE--SGRAPEDAVVQQRAHRAREIIQMLS 241
BLAST of SMED30033819 vs. Planmine SMEST
Match: SMESG000045681.1 (SMESG000045681.1) HSP 1 Score: 244.973 bits (624), Expect = 4.618e-84 Identity = 141/142 (99.30%), Postives = 141/142 (99.30%), Query Frame = 1 Query: 28 MSIDENPIEQNLDXXXXXXXXXXXXXXXXLSHKNKTSVSDINESSDIIAKIRDYLLSYEDKSNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIINDDCSGKTSKNRASKIASILNSTLQNS 453 MSIDENPIEQNLDTKKKRITNRRRKIVKRLSHKNKTSVSDINESSDIIAKIRDYLLSYE KSNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIINDDCSGKTSKNRASKIASILNSTLQNS Sbjct: 1 MSIDENPIEQNLDTKKKRITNRRRKIVKRLSHKNKTSVSDINESSDIIAKIRDYLLSYEGKSNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIINDDCSGKTSKNRASKIASILNSTLQNS 142
BLAST of SMED30033819 vs. Planmine SMEST
Match: SMESG000017524.1 (SMESG000017524.1) HSP 1 Score: 242.662 bits (618), Expect = 3.075e-83 Identity = 140/142 (98.59%), Postives = 141/142 (99.30%), Query Frame = 1 Query: 28 MSIDENPIEQNLDXXXXXXXXXXXXXXXXLSHKNKTSVSDINESSDIIAKIRDYLLSYEDKSNWKFNKVTQKALLQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIINDDCSGKTSKNRASKIASILNSTLQNS 453 MSIDENPIEQNLDTKKKRITNRRRKIVKRLSHKNKTSVSDI ESSDIIAKIRDYLLSYEDKSNWKFNKVTQKA+LQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIINDDCSGKTSKNRASKIASILNSTLQNS Sbjct: 1 MSIDENPIEQNLDTKKKRITNRRRKIVKRLSHKNKTSVSDIIESSDIIAKIRDYLLSYEDKSNWKFNKVTQKAILQNMYFKDKCDSSLFKIVKKYIQALQGASKTRVLEKCNSIINDDCSGKTSKNRASKIASILNSTLQNS 142 The following BLAST results are available for this feature:
BLAST of SMED30033819 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of SMED30033819 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30033819 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30033819 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of SMED30033819 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 2
BLAST of SMED30033819 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30033819 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of SMED30033819 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30033819 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30033819 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of SMED30033819 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30033819 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30033819 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of SMED30033819 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30033819 ID=SMED30033819|Name=SMED30033819|organism=Schmidtea mediterranea sexual|type=transcript|length=558bpback to top protein sequence of SMED30033819-orf-1 >SMED30033819-orf-1 ID=SMED30033819-orf-1|Name=SMED30033819-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=143bp MSIDENPIEQNLDTKKKRITNRRRKIVKRLSHKNKTSVSDINESSDIIAKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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