RNase H domain-containing protein

Overview
NameRNase H domain-containing protein
Smed IDSMED30033811
Length (bp)1796
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of RNase H domain-containing protein (SMED30033811) t-SNE clustered cells

Violin plots show distribution of expression levels for RNase H domain-containing protein (SMED30033811) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033811

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
neoblastSMED30033811 dd_Smed_v4_35674_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of RNase H domain-containing protein vs. TrEMBL
Match: A0A0V0SES0 (Gag-Pro-Pol polyprotein OS=Trichinella nelsoni OX=6336 GN=T07_8919 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 7.518e-43
Identity = 77/247 (31.17%), Postives = 119/247 (48.18%), Query Frame = 3 
Query:  105 ITRRIPLSLCGKLVGHYPVAA*LRVAYSYIKRIANGMKLDGRLTVTNSNCRVFWCE--------ASSLALEVVLITNGDAVEDASWLRKGQNYL*MWRKLMRC*KGISLPIQ*GVNRIEMMIDGSKVASWL*TVLNNESGNCIY*NLETLVKRRLGIIKELTADGSITIFVCLVKSAENRADVLTCVPVK*LK---LWNSF*CSKLSTEKIKERYANIIS-------------AQKSQCTSSNRKIR 773
            +TRR   S CGKLV H+PV   LR+A +YIKR AN         +     R    E        ASSLA+ V L   G  VEDA+WLR          +L    KG++L +   + +I +M D + V  W+   L+ ++      + E L++RR+G+I  L  +  + + V LVKSA N+AD LT VP + LK      +  C+  + + ++   A++               A+++  T S R++R
Sbjct:  687 LTRRSVFSYCGKLVSHFPVCGWLRIAAAYIKRKANDATTSWDEVIDGDELRELIQETALAVKKHASSLAIGVALEVGGSIVEDAAWLRPDDAQHINMAELDAVIKGLNLALSWQMRKIRLMTDSATVHRWVSDGLSGKARLKTKASGEMLIRRRVGMILSLVEEFGLELEVDLVKSACNKADELTRVPRRWLKPPAAGPALVCAATADQSVERMIADVHHAMGHPGIRRTLYFARRTDPTVSKRQVR 933

HSP 2 Score: 61.6178 bits (148), Expect = 7.518e-43
Identity = 30/64 (46.88%), Postives = 37/64 (57.81%), Query Frame = 2 
Query:  989 NLEEVFRERGPVSELLVYNRTSFQSNSFIELCWRWNIKPTYTCAYRPARNGIVERNQRTIKCNA 1180
             LE VF ERG   ELL  N T+F+   F E   RW ++  Y CAY P+ NGI ER  R++K  A
Sbjct: 1006 QLEAVFYERGAPEELLTDNDTAFRGRIFTEFVARWGVRVRYRCAYAPSGNGIAERCHRSVKVIA 1069

HSP 3 Score: 46.9802 bits (110), Expect = 7.518e-43
Identity = 22/63 (34.92%), Postives = 38/63 (60.32%), Query Frame = 1 
Query:  808 IDPLSIKW*HGILVVT*N*ESIGIDLKNYKESTFLTMINCEPSRFSVWMKIDREDAQN-TEKF 993
            +DP   KW  G L V    + +G+D+ + +   +LT+I+C PSRF+VW ++  + + N TE+ 
Sbjct:  945 LDPAPRKWKRGSLEVEEVWQRVGMDITHVRGRPYLTLIDCGPSRFAVWRRLRVQCSANVTEQL 1007

HSP 4 Score: 41.2022 bits (95), Expect = 7.518e-43
Identity = 29/90 (32.22%), Postives = 41/90 (45.56%), Query Frame = 3 
Query: 1215 LVQYAPRYGKNQDTAARRVVQ---VLLEILSKKKAES-SVTCEYEIGQRVVAKLALAKCTTRWPIRFVTGINSATNIEVDGTSKHVSDIR 1472
            L    PR G+N  TA   VV    V +  + +   E       + +G  V  +   A+C TR     VTG+ S   +EVDG  +HV D+R
Sbjct: 1082 LYNVTPRDGRNPWTAPANVVHAYAVRVRGVDRATEEPKEKNGRFAVGDSVWVRPPGARCDTRHQKGTVTGVVSDQAVEVDGMPRHVRDLR 1171
[Close]
BLAST of RNase H domain-containing protein vs. TrEMBL
Match: A0A085MC07 (Uncharacterized protein OS=Trichuris suis OX=68888 GN=M513_04453 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 7.906e-40
Identity = 81/242 (33.47%), Postives = 120/242 (49.59%), Query Frame = 3 
Query:   90 MIEKEITRRIPLSLCGKLVGHYPVAA*LRVAYSYIKRIANGM----------------------------KLDGRLTVTNSNCRVFWCEASSLALEVVLITNGDAVEDASWLRKGQNYL*MWRKLMRC*KGISLPIQ*GVNRIEMMIDGSKVASWL*TVLNNESGNCIY*NLETLVKRRLGIIKELTADGSITIFVCLVKSAENRADVLTCVPVK*LKLWN--SF*CSKLSTEKIKERYANI 725
            ++   ++RR   SLCGKLVGH PV   LRV  + IKR  N                                 G   VT    RV W +ASSLA+ V L  +G  VEDASWLR  +       +L    KG++L I   +++I+++ D S V  W+   L+ ++      + E L++RR+GI+  L  + S+ + V LVKSAEN+ADVLT VP +  ++    +  C+    E+++ R A I
Sbjct:   14 IVPPRLSRRSVFSLCGKLVGHLPVCGWLRVVTALIKRRVNDATSTWDQAIEDDELRMLLQEVVSEARSRDPARGMWDVTGDEARV-WVDASSLAIGVALEVDGAIVEDASWLRSDEASHINMAELDAVVKGLNLAISWRMSKIQLVTDSSTVHRWITDGLSGKTRLRTKASSEMLIRRRIGIVLSLVQEYSLDLSVKLVKSAENKADVLTRVPQRWSRILGTPTPVCATAIDEEVESRIAEI 254

HSP 2 Score: 62.003 bits (149), Expect = 7.906e-40
Identity = 31/64 (48.44%), Postives = 39/64 (60.94%), Query Frame = 2 
Query:  989 NLEEVFRERGPVSELLVYNRTSFQSNSFIELCWRWNIKPTYTCAYRPARNGIVERNQRTIKCNA 1180
             LE VF ERG   ELL  N T+F+S +F     RW I+  + CA+ P+ NGIVER  RT+K  A
Sbjct:  356 QLESVFCERGAPEELLTDNDTAFRSKAFSRFAARWGIRVRFRCAHVPSGNGIVERCHRTVKVIA 419

HSP 3 Score: 48.9062 bits (115), Expect = 7.906e-40
Identity = 19/51 (37.25%), Postives = 32/51 (62.75%), Query Frame = 1 
Query:  808 IDPLSIKW*HGILVVT*N*ESIGIDLKNYKESTFLTMINCEPSRFSVWMKI 960
            IDP  +KW  G L V    + +G+D+ +Y    +L++I+C PSRF++W  +
Sbjct:  295 IDPAPVKWRRGSLSVEKVWQRVGMDIAHYGGRPYLSLIDCGPSRFAIWRPL 345
[Close]
BLAST of RNase H domain-containing protein vs. TrEMBL
Match: A0A085M3Y4 (Uncharacterized protein OS=Trichuris suis OX=68888 GN=M513_07259 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 1.717e-39
Identity = 81/241 (33.61%), Postives = 119/241 (49.38%), Query Frame = 3 
Query:   93 IEKEITRRIPLSLCGKLVGHYPVAA*LRVAYSYIKRIANGM----------------------------KLDGRLTVTNSNCRVFWCEASSLALEVVLITNGDAVEDASWLRKGQNYL*MWRKLMRC*KGISLPIQ*GVNRIEMMIDGSKVASWL*TVLNNESGNCIY*NLETLVKRRLGIIKELTADGSITIFVCLVKSAENRADVLTCVPVK*LKLWN--SF*CSKLSTEKIKERYANI 725
            +   ++RR   SLCGKLVGH PV   LRV  + IKR  N                                 G   VT    RV W +ASSLA+ V L  +G  VEDASWLR  +       +L    KG++L I   +++I+++ D S V  W+   L+ ++      + E L++RR+GI+  L  + S+ + V LVKSAEN+ADVLT VP +  ++    +  C+    E+++ R A I
Sbjct:  459 VPPRLSRRSVFSLCGKLVGHLPVCGWLRVVTALIKRRVNDATSTWDQAIEDDELRMLLQEVVSEARSRDPARGMRDVTGDEARV-WVDASSLAIGVALEVDGAIVEDASWLRSDEASHINMAELDAVVKGLNLAISWRMSKIQLVTDSSTVHRWITDGLSGKTRLRTKASSEMLIRRRIGIVLSLVQEYSLDLSVKLVKSAENKADVLTRVPQRWSRILGTPTPVCATAIDEEVESRIAEI 698

HSP 2 Score: 60.8474 bits (146), Expect = 1.717e-39
Identity = 31/64 (48.44%), Postives = 38/64 (59.38%), Query Frame = 2 
Query:  989 NLEEVFRERGPVSELLVYNRTSFQSNSFIELCWRWNIKPTYTCAYRPARNGIVERNQRTIKCNA 1180
             LE VF ERG   ELL  N T+F+S  F     RW I+  + CA+ P+ NGIVER  RT+K  A
Sbjct:  800 QLESVFCERGAPEELLADNDTAFRSKVFSRFATRWGIRVRFRCAHVPSGNGIVERCHRTVKVIA 863

HSP 3 Score: 48.521 bits (114), Expect = 1.717e-39
Identity = 19/51 (37.25%), Postives = 32/51 (62.75%), Query Frame = 1 
Query:  808 IDPLSIKW*HGILVVT*N*ESIGIDLKNYKESTFLTMINCEPSRFSVWMKI 960
            IDP  +KW  G L V    + +G+D+ +Y    +L++I+C PSRF++W  +
Sbjct:  739 IDPAPVKWRRGSLSVEKVWQRVGMDIAHYGGRPYLSLIDCGPSRFTIWRPL 789
[Close]
BLAST of RNase H domain-containing protein vs. TrEMBL
Match: A0A5S6Q5U0 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 6.725e-38
Identity = 77/247 (31.17%), Postives = 114/247 (46.15%), Query Frame = 3 
Query:   75 CRSEEMIEKEITRRIPLSLCGKLVGHYPVAA*LRVAYSYIKRIANGMKLDGRLTVTNSNCRVF---------------------------WCEASSLALEVVLITNGDAVEDASWLRKGQNYL*MWRKLMRC*KGISLPIQ*GVNRIEMMIDGSKVASWL*TVLNNESGNCIY*NLETLVKRRLGIIKELTADGSITIFVCLVKSAENRADVLTCVPVK*LKLWNS---F*CSKLSTEKIKERYANI 725
            C     + +++TRR   SLCG+LVGHYPV   LRV  ++ KR  N +       V +   R F                           W +ASSLAL V+L  +G  VEDA WLR          +L    +G++L I   +  IE+M D S V  W+   L+ +S        E L++RR+  +  L A+  + + V LV+S  NRADVLT VP + +K       + C+ +  E ++    N+
Sbjct:  632 CSEISDVPRKLTRRAVFSLCGELVGHYPVCGWLRVTAAFAKRQINAVTKGWDDPVDSVAVRAFLEETVRKVRDEDPARGAWNVSGERARLWVDASSLALGVMLEVDGSVVEDAGWLRPDDAQHINMAELDALIRGLNLAIAWRMRCIEVMTDSSAVHRWISDGLSGKSRLRTKAAGEMLIRRRVSTVLSLVAEYGLELTVNLVRSETNRADVLTRVPQRIMKALTDSQRYTCAVIVDEDLRRLITNV 878

HSP 2 Score: 61.2326 bits (147), Expect = 6.725e-38
Identity = 27/60 (45.00%), Postives = 39/60 (65.00%), Query Frame = 2 
Query:  992 LEEVFRERGPVSELLVYNRTSFQSNSFIELCWRWNIKPTYTCAYRPARNGIVERNQRTIK 1171
            LE VF ERG   E+L+ N T+F+S +F +   RW ++  Y CA+ P+ NGI ER  R++K
Sbjct:  981 LENVFYERGAPEEILLDNDTAFRSRTFTDFAARWGMRVRYRCAHAPSGNGIAERCHRSVK 1040

HSP 3 Score: 47.3654 bits (111), Expect = 6.725e-38
Identity = 19/48 (39.58%), Postives = 30/48 (62.50%), Query Frame = 1 
Query:  808 IDPLSIKW*HGILVVT*N*ESIGIDLKNYKESTFLTMINCEPSRFSVW 951
            IDP  + W  G L V    + IG+D+ +Y    +LT+++C PSRF++W
Sbjct:  919 IDPAPVTWKRGNLEVDKVWQRIGVDITHYGSKPYLTIVDCGPSRFAIW 966
[Close]
BLAST of RNase H domain-containing protein vs. TrEMBL
Match: A0A085M286 (Uncharacterized protein OS=Trichuris suis OX=68888 GN=M513_07737 PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 4.210e-37
Identity = 71/245 (28.98%), Postives = 115/245 (46.94%), Query Frame = 3 
Query:   93 IEKEITRRIPLSLCGKLVGHYPVAA*LRVAYSYIKRIANGMK------------LD----------------GRLTVTNSNCRVFWCEASSLALEVVLITNGDAVEDASWLRKGQNYL*MWRKLMRC*KGISLPIQ*GVNRIEMMIDGSKVASWL*TVLNNESGNCIY*NLETLVKRRLGIIKELTADGSITIFVCLVKSAENRADVLTCVPVK*LKLWNSF*CSKLSTEKIKERYANIISAQKS 743
            + + +TRR   S CG+LVGHYPV   LR A +++KR AN M             LD                G+  V+    ++ W +AS +AL V L  NG  +ED +WLR          +L    +G++L +   +  +E+M D + V  W+   L+  +        E L++RR+  +  L  +  + + V  VKSA+NRAD+LT VP + L   +    S  +   +++    I+S  +S
Sbjct:  381 LPERLTRRTVFSYCGELVGHYPVCGWLRAATAFVKREANSMTSRWDEPIYSGRLLDHLREIAAAVKAQDPAHGKWDVSGDKAKI-WVDASVVALGVALEVNGAIIEDGAWLRPDDARHINMAELDAVIRGLNLALSWQMKDLELMTDSATVHRWIEDALSGRTRLKTRAANEMLIRRRIATVLALVKEYDLNLTVRQVKSADNRADILTRVPRRWLAPADPHGLSAGALADVEQSEKLIVSVHRS 624

HSP 2 Score: 62.003 bits (149), Expect = 4.210e-37
Identity = 28/63 (44.44%), Postives = 40/63 (63.49%), Query Frame = 2 
Query:  992 LEEVFRERGPVSELLVYNRTSFQSNSFIELCWRWNIKPTYTCAYRPARNGIVERNQRTIKCNA 1180
            +E VF ERG   E+L  N T+F+S +F ++  RW ++  + CAY P+ NGI ER  RT+K  A
Sbjct:  724 MEAVFFERGAPEEVLADNDTAFRSQAFADMAARWGLRIRFRCAYAPSGNGIAERCHRTVKVIA 786

HSP 3 Score: 48.9062 bits (115), Expect = 4.210e-37
Identity = 21/58 (36.21%), Postives = 33/58 (56.90%), Query Frame = 1 
Query:  808 IDPLSIKW*HGILVVT*N*ESIGIDLKNYKESTFLTMINCEPSRFSVWMKIDREDAQN 981
            IDP   KW  G L V    + +  D+ +++   FLTMI+C PSRF++W  +    A++
Sbjct:  662 IDPAPTKWRQGNLEVDRTWQRVSCDVTHFRGELFLTMIDCGPSRFAIWRALRSHSAKD 719
[Close]
BLAST of RNase H domain-containing protein vs. Planmine SMEST
Match: SMESG000034856.1 (SMESG000034856.1)

HSP 1 Score: 275.018 bits (702), Expect = 1.355e-79
Identity = 133/134 (99.25%), Postives = 133/134 (99.25%), Query Frame = 3 
Query: 1176 MRQESCRSSGYRLLVQYAPRYGKNQDTAARRVVQVLLEILSKKKAESSVTCEYEIGQRVVAKLALAKCTTRWPIRFVTGINSATNIEVDGTSKHVSDIRPVMDEDIADDNSKYRSKKDQARKWKVPHVKTGRDE 1577
            MRQESCRSSGYRLLVQYAPRYGKNQDTA RRVVQVLLEILSKKKAESSVTCEYEIGQRVVAKLALAKCTTRWPIRFVTGINSATNIEVDGTSKHVSDIRPVMDEDIADDNSKYRSKKDQARKWKVPHVKTGRDE
Sbjct:    1 MRQESCRSSGYRLLVQYAPRYGKNQDTAVRRVVQVLLEILSKKKAESSVTCEYEIGQRVVAKLALAKCTTRWPIRFVTGINSATNIEVDGTSKHVSDIRPVMDEDIADDNSKYRSKKDQARKWKVPHVKTGRDE 134
[Close]
BLAST of RNase H domain-containing protein vs. Planmine SMEST
Match: SMESG000069076.1 (SMESG000069076.1)

HSP 1 Score: 111.694 bits (278), Expect = 2.861e-31
Identity = 54/82 (65.85%), Postives = 68/82 (82.93%), Query Frame = 3 
Query: 1305 KAESSVTCEYEIGQRVVAKLALAKCTTRWPIRFVTGINSATNIEVDGTSKHVSDIRPVMDEDIADDNSKYRSKKDQARKWKV 1550
            + E+SVTC+YE GQ VV K A AKCTT+WPI  VTGINSAT+IEVDGTS+HVS+IRPV+ ED+ADD S+ RS+K+Q  + +V
Sbjct:  278 EVENSVTCDYETGQMVVVKPAPAKCTTQWPIGIVTGINSATDIEVDGTSRHVSNIRPVVKEDMADDRSENRSEKNQPSEIEV 359

HSP 2 Score: 36.1946 bits (82), Expect = 2.861e-31
Identity = 17/24 (70.83%), Postives = 19/24 (79.17%), Query Frame = 1 
Query: 1228 HLDMAKIRTQLPEGLFKYYWRFYR 1299
            HL+MA IRT   EGLFKY+W FYR
Sbjct:  255 HLEMAPIRT---EGLFKYHWIFYR 275

HSP 3 Score: 27.7202 bits (60), Expect = 2.861e-31
Identity = 13/15 (86.67%), Postives = 13/15 (86.67%), Query Frame = 2 
Query: 1178 APGVMQILWISSSGT 1222
            A GVMQIL ISSSGT
Sbjct:  238 ATGVMQILGISSSGT 252
[Close]
BLAST of RNase H domain-containing protein vs. Planmine SMEST
Match: SMESG000064600.1 (SMESG000064600.1)

HSP 1 Score: 52.7582 bits (125), Expect = 1.278e-22
Identity = 32/89 (35.96%), Postives = 53/89 (59.55%), Query Frame = 3 
Query: 1284 LEILSKKKAESSVTCEYEIGQRVVAKLALAKCTTRWPIRFVTGINSATNIEVDGTSKHVSDIRPVMDEDIADDNSKYRSKKDQARKWKV 1550
             E LS   A+S +T ++++G +V      A+C T   +  VTG+NS T+I VDG ++HVSDIR +  E++ +D  +  S ++   K K+
Sbjct:  281 FEFLSPSVAQS-LTFKFKLGDKVYVDPGRAQCFTPMKLGNVTGVNSETSITVDGINRHVSDIR-LASENMNEDRMESSSYEEALTKVKM 367

HSP 2 Score: 46.9802 bits (110), Expect = 1.278e-22
Identity = 22/55 (40.00%), Postives = 32/55 (58.18%), Query Frame = 1 
Query:  808 IDPLSIKW*HGILVVT*N*ESIGIDLKNYKESTFLTMINCEPSRFSVWMKIDRED 972
            +DP  IKW  G L V    + + +D  NY    FL++I+C  SRFS+W K+  E+
Sbjct:  137 VDPSPIKWLKGHLSVPEVWQRLAVDTTNYGNFVFLSVIDCGSSRFSIWRKLKSEN 191

HSP 3 Score: 46.2098 bits (108), Expect = 1.278e-22
Identity = 26/70 (37.14%), Postives = 36/70 (51.43%), Query Frame = 2 
Query:  962 TERTLKTQRNLEEVFRERGPVSELLVYNRTSFQSNSFIELCWRWNIKPTYTCAYRPARNGIVERNQRTIK 1171
            +E  +  + N   VF E G   E+L+ N  +F  N    LC    +K  Y   Y+P+ NGIVER  RT+K
Sbjct:  189 SENEIDVRDNHRSVFLEHGVPVEILLDNSRTFNFNEIRTLCELLCLKMIYRNTYQPSGNGIVERVHRTVK 258
[Close]
BLAST of RNase H domain-containing protein vs. Planmine SMEST
Match: SMESG000020506.1 (SMESG000020506.1)

HSP 1 Score: 60.4622 bits (145), Expect = 2.907e-19
Identity = 39/142 (27.46%), Postives = 69/142 (48.59%), Query Frame = 1 
Query:  877 IDLKNYKESTFLTMINCEPSRFSVWMKIDREDAQNTEKFGGSISRKRPSIRITCV**DKFSI*FLHRVMLEMEYKTNLHMRLPSR*KRD--CRKKSADDKV*CARSHADPLDIVFWYNTHLDMAKIRTQLPEG-LFKYYWRF 1293
            +D   +    FLTM++C PSRF++W+K+  +D+++  +    + R++  ++   V  +  S        L +++  NL  R   R   +    +     K   +RS +DP D +FWYN  L +   ++  P   LF Y WRF
Sbjct:    9 VDATQFNVLPFLTMVDCGPSRFAIWIKLSSKDSRSISRNLEKVFREQGPVKELLV-DNGASFRSDQIKKLCLKWSVNLSFRCAYRPSGNGIVERNHRTTKCSASRSRSDPRDDIFWYNVALKLKGDKSSAPANRLFNYGWRF 149

HSP 2 Score: 54.6842 bits (130), Expect = 2.907e-19
Identity = 24/53 (45.28%), Postives = 34/53 (64.15%), Query Frame = 3 
Query: 1332 YEIGQRVVAKLALAKCTTRWPIRFVTGINSATNIEVDGTSKHVSDIRPVMDED 1490
            +E  Q    K    KCTT WP+  +T INS TNI+VDG  +H++DIRP++  +
Sbjct:  163 FEECQSGFVKPYPFKCTTVWPMGTITAINSLTNIDVDGMPRHITDIRPMVQSE 215

HSP 3 Score: 87.0409 bits (214), Expect = 8.289e-19
Identity = 39/65 (60.00%), Postives = 51/65 (78.46%), Query Frame = 2 
Query:  986 RNLEEVFRERGPVSELLVYNRTSFQSNSFIELCWRWNIKPTYTCAYRPARNGIVERNQRTIKCNA 1180
            RNLE+VFRE+GPV ELLV N  SF+S+   +LC +W++  ++ CAYRP+ NGIVERN RT KC+A
Sbjct:   46 RNLEKVFREQGPVKELLVDNGASFRSDQIKKLCLKWSVNLSFRCAYRPSGNGIVERNHRTTKCSA 110
[Close]
BLAST of RNase H domain-containing protein vs. Planmine SMEST
Match: SMESG000019677.1 (SMESG000019677.1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.583e-18
Identity = 68/209 (32.54%), Postives = 107/209 (51.20%), Query Frame = 3 
Query:   93 IEKEITRRIPLSLCGKLVGHYPVAA*LRVAYSYIKRIANG------------------MKLDGR------LTVTNSNCRVFWCEASSLALEVVLITNGDAVEDASWLRKGQNYL*M-WRKLMRC*KGISLPIQ*GVNRIEMMIDGSKVASWL*TVLNNESGNCIY*NLETLVKRRLGIIKELTADGSITIFVCLVKSAENRADVLTCVP 644
            + K ++R+   SLCG+L G Y V   LR A SYIKR++NG                   K+D R       +V ++   + WC+ASS+A+ +VL      VEDA WLRK  +   +   +L    KG++L I+  +  I++  D   V  W+  VL+N+       N E L++ RL ++K L+ +  + + +  V S  N+A+VLT VP
Sbjct:  127 LNKGLSRKELYSLCGQLAGIYRVTGWLRPACSYIKRLSNGNWDDPIENHIVEKVKWLLEKVDERDPTSGIWSVPSNQTGIIWCDASSIAIGIVLEMEDAMVEDACWLRKPDDSRHINLVELEAIVKGVNLCIKRNLKYIKIKSDSKSVVCWVNKVLSNDRKIHTKANSELLIRPRLKVLKNLSIEYELKMDIEFVSSENNQANVLTRVP 335
The following BLAST results are available for this feature:
BLAST of RNase H domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNase H domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNase H domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNase H domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNase H domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNase H domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNase H domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNase H domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0V0SES07.518e-4331.17Gag-Pro-Pol polyprotein OS=Trichinella nelsoni OX=... [more]
A0A085MC077.906e-4033.47Uncharacterized protein OS=Trichuris suis OX=68888... [more]
A0A085M3Y41.717e-3933.61Uncharacterized protein OS=Trichuris suis OX=68888... [more]
A0A5S6Q5U06.725e-3831.17Uncharacterized protein OS=Trichuris muris OX=7041... [more]
A0A085M2864.210e-3728.98Uncharacterized protein OS=Trichuris suis OX=68888... [more]
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BLAST of RNase H domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNase H domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNase H domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNase H domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNase H domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of RNase H domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000034856.11.355e-7999.25SMESG000034856.1[more]
SMESG000069076.12.861e-3165.85SMESG000069076.1[more]
SMESG000064600.11.278e-2235.96SMESG000064600.1[more]
SMESG000020506.12.907e-1927.46SMESG000020506.1[more]
SMESG000019677.11.583e-1832.54SMESG000019677.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033811 ID=SMED30033811|Name=RNase H domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1796bp
AGCTCAATATGGCTTGACTACAAATTATCAGAGTCTTGGGCTCAGAATAA
AACATGATCGTTGTGGCCACCTGTTGTCGATCAGAGGAAATGATCGAAAA
GGAAATCACTCGTCGTATACCGCTTTCTTTATGCGGTAAGCTTGTTGGGC
ATTATCCAGTTGCAGCGTAGCTCAGAGTAGCCTACAGTTATATTAAAAGA
ATTGCAAACGGTATGAAATTGGATGGAAGGTTGACTGTAACTAATAGTAA
TTGCAGGGTTTTTTGGTGTGAAGCGAGTAGTCTCGCTTTAGAGGTTGTGC
TGATAACAAATGGCGATGCTGTTGAAGACGCTTCGTGGCTCAGAAAAGGC
CAGAATTACTTATAAATGTGGCGGAAATTGATGCGGTGCTAAAAAGGAAT
TAGCTTGCCAATTCAATGAGGAGTTAATAGAATCGAAATGATGATAGATG
GATCAAAGGTAGCAAGCTGGTTATAAACAGTGCTGAATAATGAAAGCGGA
AATTGTATCTATTGAAATTTAGAAACGCTTGTTAAGCGCCGCCTAGGAAT
AATCAAGGAACTAACTGCCGACGGAAGTATAACTATTTTTGTATGTTTAG
TGAAGTCAGCAGAGAACCGAGCAGATGTCCTCACGTGCGTTCCAGTGAAA
TAACTGAAGCTTTGGAATTCCTTCTGATGTTCCAAACTGTCAACTGAGAA
AATAAAAGAACGCTACGCAAACATCATTTCGGCTCAGAAAAGTCAATGTA
CTTCATCCAATCGGAAAATCCGGAAACCGATCAAGAAAGCATACAGAATA
CAGTATAATCGATCCATTATCGATTAAATGGTAGCATGGGATACTAGTGG
TAACGTAAAATTGAGAGAGTATAGGGATTGATCTCAAAAACTACAAGGAA
TCCACATTTTTGACAATGATAAACTGTGAACCAAGCAGGTTTTCAGTGTG
GATGAAAATAGACCGAGAGGACGCTCAAAACACAGAGAAATTTGGAGGAA
GTATTTCGAGAAAGAGGCCCAGTATCAGAATTACTTGTGTATAATAGGAC
AAGTTTTCAATCTAATTCCTTCATAGAGTTATGTTGGAGATGGAATATAA
AACCAACCTACACATGCGCTTACCGTCCCGCTAGAAACGGGATTGTAGAA
AGAAATCAGCGGACGATAAAGTGTAATGCGCCAGGAGTCATGCAGATCCT
CTGGATATCGTCTTCTGGTACAATACGCACCTAGATATGGCAAAAATCAG
GACACAGCTGCCAGAAGGGTTGTTCAAGTATTACTGGAGATTTTATCGAA
GAAAAAAGCTGAGAGTTCAGTAACATGCGAATATGAAATTGGTCAAAGAG
TTGTGGCTAAGCTTGCACTAGCAAAGTGTACTACTCGATGGCCCATTCGA
TTTGTGACTGGAATTAATTCAGCCACAAACATTGAAGTAGATGGGACATC
GAAACACGTATCGGACATCCGACCTGTAATGGACGAAGATATAGCTGACG
ATAATTCCAAATATCGATCTAAGAAAGACCAAGCAAGGAAATGGAAGGTG
CCACATGTGAAGACTGGTCGAGACGAGTAAGTCGCTTTATCTTGATCAGT
ATGGCTTGTATACATCGTACTGAGGAATTTTTAATGTTTTTTGTTTATTT
TGACACTGTTGCAAAATAGTGTTGAACCGTTGATAATCGCTGAGTTATAT
TATAGAAAGAACGAAATTTGAAGACATTTCAGATAACTCTTCTATGTTAA
TAAAAAATGAAATAATTTCTTAAGATTTTTCATGACATTCAATCAA
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protein sequence of SMED30033811-orf-1

>SMED30033811-orf-1 ID=SMED30033811-orf-1|Name=SMED30033811-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=135bp
MRQESCRSSGYRLLVQYAPRYGKNQDTAARRVVQVLLEILSKKKAESSVT
CEYEIGQRVVAKLALAKCTTRWPIRFVTGINSATNIEVDGTSKHVSDIRP
VMDEDIADDNSKYRSKKDQARKWKVPHVKTGRDE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004523RNA-DNA hybrid ribonuclease activity
GO:0003676nucleic acid binding
Vocabulary: biological process
TermDefinition
GO:0090502RNA phosphodiester bond hydrolysis, endonucleolytic
GO:0015074DNA integration
Vocabulary: INTERPRO
TermDefinition
IPR001584Integrase_cat-core
IPR012337RNaseH-like_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 106..134