Mannosyl phosphorylinositol ceramide synthase SUR1

Overview
NameMannosyl phosphorylinositol ceramide synthase SUR1
Smed IDSMED30033783
Length (bp)867
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Mannosyl phosphorylinositol ceramide synthase SUR1 (SMED30033783) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Mannosyl phosphorylinositol ceramide synthase SUR1 (SMED30033783) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033783

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
non-ciliated neuronSMED30033783h1SMcG0004311 dd_Smed_v4_78546_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. UniProt/SwissProt
Match: sp|P33300|SUR1_YEAST (Mannosyl phosphorylinositol ceramide synthase SUR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUR1 PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.042e-22
Identity = 56/182 (30.77%), Postives = 89/182 (48.90%), Query Frame = 2 
Query:   86 PNRIPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQ------SSSVHQTGPIMLTGTFKSYKK 613
            P  IPKIIH  YKT  IP  +    + C++++ D K++ W+D MAY F+   +P +L+     F N+   ++ +D IRYFIL  +GG+Y+D D  C++        D  +       +T      P    N +M  V  H FF   +  L   D+       + +  TGP+ L+  +K YK+
Sbjct:   52 PQLIPKIIHQTYKTEDIPEHWKEGRQKCLDLHPDYKYILWTDEMAYEFIKEEYPWFLDT----FENYKYPIERADAIRYFILSHYGGVYIDLDDGCERKL------DPLLAFPAFLRKTS-----PLGVSNDVMGSVPRHPFFLKALKSLKHYDKYWFIPYMTIMGSTGPLFLSVIWKQYKR 218
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. UniProt/SwissProt
Match: sp|P38287|CSH1_YEAST (Mannosyl phosphorylinositol ceramide synthase CSH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CSH1 PE=1 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 7.126e-21
Identity = 57/192 (29.69%), Postives = 97/192 (50.52%), Query Frame = 2 
Query:   86 PNRIPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQS------SSVHQTGPIMLTGTFKSYKKSTRQLKNKNI 643
            P  IPKIIH  YKT+ IP Q+V   + C++++ D  ++ W+D M+  F+   +P +L+ + SY       ++ +D IRYFIL  +GGIY+D D  C++  RL    D  +       +T      P    N +M  V  H FF  ++  L    ++      + +  TGP+ ++  +K YK+ +   +N  +
Sbjct:   59 PQLIPKIIHQTYKTNDIPEQWVKGRQKCIDLHPDYTYILWTDEMSDTFIKQEYPWFLDTFRSY----EYPIERADAIRYFILSHYGGIYIDLDDGCER--RL----DPLLKVPAFLRKTS-----PTGVSNDVMGSVPRHPFFLKVIKSLKHYKKNWYIPYMTIMGSTGPLFISVVWKQYKRWSNTAENGAV 235
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. UniProt/SwissProt
Match: sp|Q9UT67|IMT2_SCHPO (Inositol phosphoceramide mannosyltransferase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC4F11.04c PE=3 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 2.274e-19
Identity = 54/186 (29.03%), Postives = 101/186 (54.30%), Query Frame = 2 
Query:   95 IPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQS------SSVHQTGPIMLTGTFKSYKKSTRQLKN 634
            IPKIIH  +KT+ +P  +    ++C++++ D +++ W+D M+  F+ +++P +L  + +Y  N    ++ +DVIRYF+L  +GG Y+D D  C++  RL    DS +       +T      P    N +M  V +H +F+ ++  L  + +S      + +  TGP+ ++  ++ YK   RQL N
Sbjct:   82 IPKIIHQTWKTTEVPEGWKGAQQSCIDLHPDYEYILWTDEMSRNFIADNYPWFLPYFDAYPFN----VQRADVIRYFVLYHYGGNYIDLDDGCRQ--RL----DSLLYYPVWVRRTD-----PVGVSNDVMGSVPHHPYFELIIQNLEKNAKSYWLPYLTIMLSTGPLSISFLWEKYK---RQLPN 249
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. UniProt/SwissProt
Match: sp|Q10323|IMT3_SCHPO (Inositol phosphoceramide mannosyltransferase 3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC17G8.11c PE=1 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.245e-18
Identity = 52/179 (29.05%), Postives = 89/179 (49.72%), Query Frame = 2 
Query:   95 IPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQS------SSVHQTGPIMLTGTFKSYKK 613
            IPKIIH  +KT+ IP ++V   K+C++++ D ++V W+D     F+   +P +L  Y SY  N    ++ +DV+RYFIL K+GGIY+D D  C +            L   +     +    P    N++M     H F   +V  L     +      + ++ TGP+ L+  + +++K
Sbjct:   57 IPKIIHQTWKTNEIPEKWVGAQKSCIDLHPDYEYVLWTDESMREFIATDYPWFLTQYDSYPYN----IERADVVRYFILYKYGGIYLDLDVGCNR-----------TLDPLLHYPAWVRRTSPSGISNNVMGFAKGHPFLLQVVRNLPRFAFNYHFPYLTVMYSTGPLFLSIIWSAWRK 220
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. UniProt/SwissProt
Match: sp|Q5UQW4|YL373_MIMIV (Uncharacterized glycosyltransferase L373 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L373 PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 6.949e-12
Identity = 60/207 (28.99%), Postives = 90/207 (43.48%), Query Frame = 2 
Query:   95 IPKIIHYVYKTSF--IPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHF-PEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSR--LNISDQSSSVHQTGPIMLTGTFKSYKKSTRQLKNKNISQKISLANSYLFSPYSVGN 700
            IP IIH ++   +  IP++     + C++INY  K  FW +     F+ N+F PEYL +Y  Y        + +D  RY IL   GGIY+D D  C+K       N    L          L NV    +N ++    NH  F Y+     L  +D S+  + TG  +   +   Y K+       N +  ISL +S    P ++ N
Sbjct:    2 IPNIIHQIWIQGYESIPSELRKYHENCLKINYGFKNEFWDNDRIRNFLKNNFEPEYLELYDKY----KIYAQKADFARYAILKIHGGIYLDMDMVCRK-------NLGDFLGLGFFFTAYKLKNVFTNYLNGVIGSRPNHPVFDYIFKNMFLRQNDASNVTNSTGTKLFRDSITEYTKN-------NPTNDISLIDSKYLHPCNLYN 190
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. TrEMBL
Match: A0A267FUE4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019369g1 PE=4 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 2.010e-39
Identity = 86/261 (32.95%), Postives = 127/261 (48.66%), Query Frame = 2 
Query:   59 IEHGNLFQFPNRIPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQSSSVH-QTGPIMLTGTFKSYKKSTRQLKNKNISQKISLANSYLFSPYSVGNLESLCNYDLNHQWKIYGCLNLKAHREIQKRKMTEAVVLHLFLHLGY 838
            IE G    F   IP I+H+ + +  +   Y  ++ +C+++N +  +  W D  A   +  H+P+Y+  Y      F D L+ SD IRY IL +FGGIY+D D +C +PF       +  L +E  EQT+I W   F AMNSLM  V  H+FFQ +  R+ +       +  TGP +LT  F+ ++ S   + N  +        S L  P    N   LC Y   + W   GC  L+A +     +    V +H FLHLGY
Sbjct:   52 IEGGTTAHF--EIPHILHHTFASEMVYENYRPMISSCLKLNPNWTYYLWRDSDARALIFLHYPQYMKTYM----RFNDNLQRSDAIRYIILHRFGGIYLDMDVECVRPFHPALTRQAAFLDQERVEQTRIAWGRQFSAMNSLMGSVPGHKFFQAMSDRVLLRKLPGDAYSSTGPNVLTQVFQRFRNSP-AIWNWPVHLLPPSVASPLMDPNR--NWRQLCRY-ARYGWGSRGCRMLRAKKWRMDDRNGGTVAVHRFLHLGY 302
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. TrEMBL
Match: A0A267FEG6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig026308g1 PE=4 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 9.419e-39
Identity = 79/255 (30.98%), Postives = 131/255 (51.37%), Query Frame = 2 
Query:   95 IPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLN-ISDQSSSVHQTGPIMLTGTFKSYKKSTRQLKNKNISQKISLANSYLFSPY--SVGNLESLCNYDLNHQW---KIYGCLNLKAHREIQKRKMTEAVVLHLFLHLGYK 841
            IPKIIH  + T  +   Y+ L+ +C+ +N D ++  W+D  A   + + +P +L+ Y SY     D L+ +D IRY +L  FGGIY+D D +CQ+PF          + +E +EQ+ I W++P+ AMNS M     H F+Q++++ ++      ++   TGP +LT  +  Y   +R+  N      ++L  S + SP      + +  C   LN +W    + GC  L+A            + +H FLHLGY+
Sbjct:   82 IPKIIHQTFPTKMVYKNYLPLINSCLTLNPDWQYYLWTDSDAEALIASKYPGFLSKYLSY----KDNLERADSIRYIVLHHFGGIYLDMDVECQRPFFPALSGLPAFVDQERQEQSNIFWSMPYSAMNSAMGSAPGHPFYQFMINAMSQYGKVGNAFESTGPRLLTAKYAEYCALSRKASNSAKLPPVALLESRVMSPLMDPGKDWKQTCLVTLNFRWWTVSLAGCKTLQAKNWRMDDSDNATLAVHKFLHLGYR 332
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. TrEMBL
Match: A0A1I8GB54 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017105g1 PE=4 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 9.722e-39
Identity = 79/255 (30.98%), Postives = 131/255 (51.37%), Query Frame = 2 
Query:   95 IPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLN-ISDQSSSVHQTGPIMLTGTFKSYKKSTRQLKNKNISQKISLANSYLFSPY--SVGNLESLCNYDLNHQW---KIYGCLNLKAHREIQKRKMTEAVVLHLFLHLGYK 841
            IPKIIH  + T  +   Y+ L+ +C+ +N D ++  W+D  A   + + +P +L+ Y SY     D L+ +D IRY +L  FGGIY+D D +CQ+PF          + +E +EQ+ I W++P+ AMNS M     H F+Q++++ ++      ++   TGP +LT  +  Y   +R+  N      ++L  S + SP      + +  C   LN +W    + GC  L+A            + +H FLHLGY+
Sbjct:   82 IPKIIHQTFPTKMVYKNYLPLINSCLTLNPDWQYYLWTDSDAEALIASKYPGFLSKYLSY----KDNLERADSIRYIVLHHFGGIYLDMDVECQRPFFPALSGLPAFVDQERQEQSNIFWSMPYSAMNSAMGSAPGHPFYQFMINAMSQYGKVGNAFESTGPRLLTAKYAEYCALSRKASNSAKLPPVALLESRVMSPLMDPGKDWKQTCLVTLNFRWWTVSLAGCKTLQAKNWRMDDSDNATLAVHKFLHLGYR 332
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. TrEMBL
Match: A0A267H5Y6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000626g1 PE=4 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 6.673e-38
Identity = 92/289 (31.83%), Postives = 145/289 (50.17%), Query Frame = 2 
Query:   95 IPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVF---------------VNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSR-LNISDQSSSVHQTGPIMLTGTFKSYKKSTRQLKNKNISQKISLANSYLFSPY---SVGNLESLCN-----------YDLNHQWKIYGCLNLKAHREIQKRKMTEAVVLHLFLHLGYKD---SKQKIN 862
            IPKI+HYV+K+  + + Y   +K+C++ N D + +FWSD  + + + +H+  +++ Y SY     D L+ SD +RY IL KFGG+Y+D D +C+KPF  +F               V  S  L +E  EQT+IL+   + AMNS+M     H FFQ  + R L +   + +   TGP  LTG ++ +   +    +    + + L  + L SP    S+ N ES C             +   +W+  GC  L A+     +     V +H FLHLGY +   +K+K N
Sbjct:   78 IPKILHYVFKSEQVFDSYKPFIKSCLDQNPDWQVLFWSDSHSEKLIADHYARFIHKYRSY----RDRLEKSDSVRYMILHKFGGVYLDMDVECRKPFSRLFTQPPVQESLAVASATVAPSAFLGEERREQTRILYGYSYCAMNSVMGSRPGHPFFQLAIDRMLGMPMLAHARLTTGPDFLTGLYRMWLNCSAGPASCQAKEPVLLYPARLVSPLMDTSLVNWESKCRPFLSKGKPDAKLEGRRKWEAMGCSQLVANGWNYDQTDDTTVAVHRFLHLGYGNQGLNKRKFN 362
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. TrEMBL
Match: A0A267FYT6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015448g3 PE=4 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 6.727e-38
Identity = 91/287 (31.71%), Postives = 143/287 (49.83%), Query Frame = 2 
Query:   95 IPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVN---------------DSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSR-LNISDQSSSVHQTGPIMLTGTFKSYKKSTRQLKNKNISQKISLANSYLFSPY---SVGNLESLCN-----------YDLNHQWKIYGCLNLKAHREIQKRKMTEAVVLHLFLHLGYKDSKQKINN 865
            IPKI+HYV+K+  + + Y   +K+C++ N D + +FWSD  + + + +H+  +++ Y SY     D L+ SD +RY IL KFGG+Y+D D +C+KPF  +F                  S  L +E  EQT+IL+   + AMNS+M     H FFQ  + R L +   + +   TGP  LTG ++ +   +    +    + + L  + L SP    S+ N ES C             +   +W+  GC  L A+     +     V +H FLHLGY +  Q +NN
Sbjct:   92 IPKILHYVFKSEQVFDSYKPFIKSCLDQNPDWQVLFWSDSHSEKLIADHYARFIHKYRSY----RDRLEKSDSVRYMILHKFGGVYLDMDVECRKPFSRLFTQPPVQESLAVASATAAPSAFLGEERREQTRILYGYSYCAMNSMMGSRPGHPFFQLAIDRMLGMPMLAHARLTTGPDFLTGLYRMWLNCSAGPASCQAKEPVLLYPARLVSPLMDTSLVNWESKCRPFFSKGKPDAKLEGRRKWEAMGCSQLVANGWNYDQTDDTTVAVHRFLHLGYGN--QGLNN 372
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Ensembl Yeast
Match: SUR1 (Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication [Source:SGD;Acc:S000005978])

HSP 1 Score: 98.9821 bits (245), Expect = 2.941e-24
Identity = 56/182 (30.77%), Postives = 89/182 (48.90%), Query Frame = 2 
Query:   86 PNRIPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQ------SSSVHQTGPIMLTGTFKSYKK 613
            P  IPKIIH  YKT  IP  +    + C++++ D K++ W+D MAY F+   +P +L+     F N+   ++ +D IRYFIL  +GG+Y+D D  C++        D  +       +T      P    N +M  V  H FF   +  L   D+       + +  TGP+ L+  +K YK+
Sbjct:   52 PQLIPKIIHQTYKTEDIPEHWKEGRQKCLDLHPDYKYILWTDEMAYEFIKEEYPWFLDT----FENYKYPIERADAIRYFILSHYGGVYIDLDDGCERKL------DPLLAFPAFLRKTS-----PLGVSNDVMGSVPRHPFFLKALKSLKHYDKYWFIPYMTIMGSTGPLFLSVIWKQYKR 218
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Ensembl Yeast
Match: CSH1 (Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication [Source:SGD;Acc:S000000365])

HSP 1 Score: 94.7449 bits (234), Expect = 1.026e-22
Identity = 57/192 (29.69%), Postives = 97/192 (50.52%), Query Frame = 2 
Query:   86 PNRIPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQS------SSVHQTGPIMLTGTFKSYKKSTRQLKNKNI 643
            P  IPKIIH  YKT+ IP Q+V   + C++++ D  ++ W+D M+  F+   +P +L+ + SY       ++ +D IRYFIL  +GGIY+D D  C++  RL    D  +       +T      P    N +M  V  H FF  ++  L    ++      + +  TGP+ ++  +K YK+ +   +N  +
Sbjct:   59 PQLIPKIIHQTYKTNDIPEQWVKGRQKCIDLHPDYTYILWTDEMSDTFIKQEYPWFLDTFRSY----EYPIERADAIRYFILSHYGGIYIDLDDGCER--RL----DPLLKVPAFLRKTS-----PTGVSNDVMGSVPRHPFFLKVIKSLKHYKKNWYIPYMTIMGSTGPLFISVVWKQYKRWSNTAENGAV 235
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Planmine SMEST
Match: SMESG000052805.1 (SMESG000052805.1)

HSP 1 Score: 596.275 bits (1536), Expect = 0.000e+0
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 2 
Query:    5 FSADIPIGFCSIITSDNLIEHGNLFQFPNRIPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQSSSVHQTGPIMLTGTFKSYKKSTRQLKNKNISQKISLANSYLFSPYSVGNLESLCNYDLNHQWKIYGCLNLKAHREIQKRKMTEAVVLHLFLHLGYKDSKQKINN 865
            FSADIPIGFCSIITSDNLIEHGNLFQFPNRIPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQSSSVHQTGPIMLTGTFKSYKKSTRQLKNKNISQKISLANSYLFSPYSVGNLESLCNYDLNHQWKIYGCLNLKAHREIQKRKMTEAVVLHLFLHLGYKDSKQKINN
Sbjct:   43 FSADIPIGFCSIITSDNLIEHGNLFQFPNRIPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQSSSVHQTGPIMLTGTFKSYKKSTRQLKNKNISQKISLANSYLFSPYSVGNLESLCNYDLNHQWKIYGCLNLKAHREIQKRKMTEAVVLHLFLHLGYKDSKQKINN 329
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Planmine SMEST
Match: SMESG000052405.1 (SMESG000052405.1)

HSP 1 Score: 318.931 bits (816), Expect = 7.046e-109
Identity = 151/278 (54.32%), Postives = 204/278 (73.38%), Query Frame = 2 
Query:   26 GFCSIITSDNLIEHGNLFQFPNRIPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQSSSVHQTGPIMLTGTFKSYKKSTRQLKNKNISQKISLANSYLFSPYSVGNLESLCNYDLNHQWKIYGCLNLKAHREIQKRKMTEAVVLHLFLHLGYKDSKQKI 859
            G C I ++++L+E G   Q+ N IPKIIHYVYKT++IP QY   +K CM+IN D KFVFW+D  AYRF++N+F  Y+ +YSSYFS  T  LKISDVIRYFIL KFGGIYMDFDT+CQKPF +  +N SCILS+EV EQT+ILWN+P+LAMNS MA + +H FF++L+ +L ++D S  + QTGP +L+ +  +YKK       K++ + ++LA+ Y +SP++   LESLC    +  WKI GC +L +HR+ QK KM EAVV+HLF+HLGY+ +K  I
Sbjct:   33 GVCEIFSTNSLMEFGRRHQYANGIPKIIHYVYKTAYIPTQYSPHVKNCMDINKDAKFVFWADFTAYRFIINNFHNYVKLYSSYFSKSTHSLKISDVIRYFILYKFGGIYMDFDTRCQKPFNIAMLNGSCILSQEVLEQTRILWNLPYLAMNSFMAAIPDHDFFKFLIGKLTLADSSFILSQTGPFLLSNSLDAYKKIITN--TKDVKKSVTLADPYFYSPHTENYLESLCK-SPSGFWKIQGCKDLGSHRQQQKLKMNEAVVVHLFMHLGYEFAKGNI 307
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Planmine SMEST
Match: SMESG000005599.1 (SMESG000005599.1)

HSP 1 Score: 318.161 bits (814), Expect = 1.370e-108
Identity = 157/279 (56.27%), Postives = 198/279 (70.97%), Query Frame = 2 
Query:   26 GFCSIITSDNLIEHGNLFQFPNRIPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQSSSVHQTGPIMLTGTFKSY-KKSTRQLKNKNISQKISLANSYLFSPYSVGNLESLCNYDLNHQWKIYGCLNLKAHREIQKRKMTEAVVLHLFLHLGYKDSKQKI 859
            G C +I+   L+E G   Q+ N IPKIIHYVYKT++IP QY + +K C  IN D KFVFW+D  AYRF++N+F +Y+ +YSSYFS  T  LKISDVIRYFIL KFGGIYMD DT+CQKPF +  +N SCILS+EV EQT+ILWN P+LAMNS MA + +H FF+YL+ +L ++D S  + QTGP MLT T +SY K  + Q K KN    +SLAN YL+SP +   LE LC +    +WK  GC +L  HR+ QK KM EAV +HLF+HLGY  +K KI
Sbjct:   33 GHCDVISMKGLLEFGRRHQYANGIPKIIHYVYKTAYIPTQYSSHIKNCKAINKDAKFVFWTDFTAYRFIINNFLDYVKLYSSYFSKSTHSLKISDVIRYFILYKFGGIYMDLDTRCQKPFDMAMLNGSCILSQEVHEQTRILWNRPYLAMNSFMAVIPDHDFFKYLIEKLTMADSSPILSQTGPFMLTNTLESYIKLISHQTKPKN---SVSLANPYLYSPLTEHYLERLCKFQYT-KWKRKGCTHLNNHRQQQKVKMKEAVAIHLFMHLGYSFAKAKI 307
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Planmine SMEST
Match: SMESG000054422.1 (SMESG000054422.1)

HSP 1 Score: 316.235 bits (809), Expect = 6.964e-108
Identity = 151/278 (54.32%), Postives = 202/278 (72.66%), Query Frame = 2 
Query:   26 GFCSIITSDNLIEHGNLFQFPNRIPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQSSSVHQTGPIMLTGTFKSYKKSTRQLKNKNISQKISLANSYLFSPYSVGNLESLCNYDLNHQWKIYGCLNLKAHREIQKRKMTEAVVLHLFLHLGYKDSKQKI 859
            G C I ++++L+E G   Q+ N IPKIIHYVYKT+FIP QY   +K CM+IN D KFVFW+D  AYRF++N+F  Y+ +YSSYFS  T  LKISDVIRYFIL KFGGIYMDFDT+CQK F +  +N SCILS+EV EQT+ILWN+P+LAMNS MA + +H FF+YL+ +L ++D S  + QTGP +L+ +  +YK        K++ + ++LA+ Y +SP++   LESLC    +  WKI GC +L +HR+ QK KM EAVV+HLF+HLGY+ +K  I
Sbjct:   33 GVCEIFSTNSLMEFGRRHQYVNGIPKIIHYVYKTAFIPTQYSPHVKNCMDINKDAKFVFWADFTAYRFIINNFHNYVKLYSSYFSKSTHSLKISDVIRYFILYKFGGIYMDFDTRCQKSFDMAMLNGSCILSQEVHEQTRILWNLPYLAMNSFMAAIPDHDFFKYLIGKLTLADSSFILSQTGPFLLSNSLDAYKNIVTN--TKDVKKSVTLADPYFYSPHTENYLESLCR-SPSGFWKIQGCKDLGSHRQQQKLKMNEAVVVHLFMHLGYEFAKGNI 307
[Close]
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Planmine SMEST
Match: SMESG000054245.1 (SMESG000054245.1)

HSP 1 Score: 316.235 bits (809), Expect = 6.964e-108
Identity = 149/278 (53.60%), Postives = 202/278 (72.66%), Query Frame = 2 
Query:   26 GFCSIITSDNLIEHGNLFQFPNRIPKIIHYVYKTSFIPNQYVALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILKISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQILWNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQSSSVHQTGPIMLTGTFKSYKKSTRQLKNKNISQKISLANSYLFSPYSVGNLESLCNYDLNHQWKIYGCLNLKAHREIQKRKMTEAVVLHLFLHLGYKDSKQKI 859
            G C I +++ L+E G   Q+ N IPKIIHY+YKT+FIP QY   +K CM+IN D KFVFW+D  AYRF++N+F  Y+ +YSSYFS  T  LKISDVIRYFIL KFGGIYMDFDT+CQKPF +  +N SCILS+EV EQ +I+WN+P+LAMNS MA + +H FF+YL+ +L ++D S  + QTGP +L+ +  +YKK       K++ + ++LA+ Y +SP++   LESLC    +  WKI GC +L +HR+ QK KM +AVV+HLF+HLGY+ +K  I
Sbjct:   33 GVCEIFSTNLLMEFGRRHQYANGIPKIIHYIYKTAFIPTQYSPHVKNCMDINKDAKFVFWADFTAYRFIINNFHNYVKLYSSYFSKSTHSLKISDVIRYFILYKFGGIYMDFDTRCQKPFDMAMLNGSCILSQEVHEQKRIMWNLPYLAMNSFMAAIPDHDFFKYLIGKLTLADSSFILSQTGPFLLSNSLDAYKKIVTN--TKDVKKSVTLADPYFYSPHTENYLESLCR-SPSGFWKIQGCKDLGSHRQQQKLKMNDAVVVHLFMHLGYEFAKGNI 307
The following BLAST results are available for this feature:
BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P33300|SUR1_YEAST2.042e-2230.77Mannosyl phosphorylinositol ceramide synthase SUR1... [more]
sp|P38287|CSH1_YEAST7.126e-2129.69Mannosyl phosphorylinositol ceramide synthase CSH1... [more]
sp|Q9UT67|IMT2_SCHPO2.274e-1929.03Inositol phosphoceramide mannosyltransferase 2 OS=... [more]
sp|Q10323|IMT3_SCHPO1.245e-1829.05Inositol phosphoceramide mannosyltransferase 3 OS=... [more]
sp|Q5UQW4|YL373_MIMIV6.949e-1228.99Uncharacterized glycosyltransferase L373 OS=Acanth... [more]
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BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267FUE42.010e-3932.95Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FEG69.419e-3930.98Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GB549.722e-3930.98Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267H5Y66.673e-3831.83Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FYT66.727e-3831.71Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
SUR12.941e-2430.77Mannosylinositol phosphorylceramide (MIPC) synthas... [more]
CSH11.026e-2229.69Mannosylinositol phosphorylceramide (MIPC) synthas... [more]
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BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mannosyl phosphorylinositol ceramide synthase SUR1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000052805.10.000e+0100.00SMESG000052805.1[more]
SMESG000052405.17.046e-10954.32SMESG000052405.1[more]
SMESG000005599.11.370e-10856.27SMESG000005599.1[more]
SMESG000054422.16.964e-10854.32SMESG000054422.1[more]
SMESG000054245.16.964e-10853.60SMESG000054245.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033783 ID=SMED30033783|Name=Mannosyl phosphorylinositol ceramide synthase SUR1|organism=Schmidtea mediterranea sexual|type=transcript|length=867bp
GATCTTCTCAGCAGATATTCCGATTGGTTTTTGCTCTATTATAACAAGTG
ACAATCTCATTGAGCATGGGAACCTGTTTCAGTTTCCAAATCGAATTCCG
AAAATAATTCATTATGTGTACAAAACTTCTTTCATTCCAAACCAATATGT
AGCACTCATGAAAACTTGTATGGAAATTAACTATGATGTGAAATTTGTGT
TCTGGAGTGACCTCATGGCCTATAGGTTCATGATGAACCATTTCCCAGAA
TATCTCAATGTTTACAGCAGTTATTTTTCTAATTTCACCGACATCCTCAA
AATATCAGATGTTATCAGATATTTTATTCTGTTGAAATTTGGAGGGATTT
ATATGGATTTTGATACAAAATGTCAAAAACCTTTTCGATTAGTTTTTGTA
AATGATTCCTGCATATTGAGCAAAGAAGTCGAAGAGCAAACTCAAATTCT
ATGGAATGTTCCTTTTCTAGCAATGAACAGTCTCATGGCTTGTGTTTCCA
ACCATCAATTCTTTCAATATCTTGTCTCTAGATTGAATATTTCTGACCAG
TCTTCGTCTGTACATCAAACCGGTCCAATTATGCTCACTGGTACGTTTAA
ATCATATAAAAAGTCCACACGTCAACTAAAAAATAAAAATATTTCCCAGA
AAATTAGCCTTGCCAATTCTTACTTGTTTTCTCCTTATTCTGTTGGTAAC
CTGGAAAGTCTGTGTAATTATGATTTGAACCACCAGTGGAAAATTTACGG
CTGCCTCAATTTAAAAGCACATCGAGAGATACAAAAACGAAAAATGACAG
AGGCTGTTGTGCTGCATTTGTTTTTGCATTTAGGATATAAAGATAGTAAA
CAGAAAATAAATAACGT
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protein sequence of SMED30033783-orf-1

>SMED30033783-orf-1 ID=SMED30033783-orf-1|Name=SMED30033783-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=288bp
IFSADIPIGFCSIITSDNLIEHGNLFQFPNRIPKIIHYVYKTSFIPNQYV
ALMKTCMEINYDVKFVFWSDLMAYRFMMNHFPEYLNVYSSYFSNFTDILK
ISDVIRYFILLKFGGIYMDFDTKCQKPFRLVFVNDSCILSKEVEEQTQIL
WNVPFLAMNSLMACVSNHQFFQYLVSRLNISDQSSSVHQTGPIMLTGTFK
SYKKSTRQLKNKNISQKISLANSYLFSPYSVGNLESLCNYDLNHQWKIYG
CLNLKAHREIQKRKMTEAVVLHLFLHLGYKDSKQKINN
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR007577GlycoTrfase_DXD_sugar-bd_CS
IPR029044Nucleotide-diphossugar_trans
Vocabulary: molecular function
TermDefinition
GO:0000030mannosyltransferase activity
GO:0016740transferase activity
GO:0016757transferase activity, transferring glycosyl groups
Vocabulary: cellular component
TermDefinition
GO:0005783endoplasmic reticulum
GO:0005789endoplasmic reticulum membrane
GO:0005794Golgi apparatus
GO:0016020membrane
GO:0016021integral component of membrane
GO:0032588trans-Golgi network membrane
GO:0033106cis-Golgi network membrane
Vocabulary: biological process
TermDefinition
GO:0051999mannosyl-inositol phosphorylceramide biosynthetic process
GO:0097502mannosylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007577Glycosyltransferase, DXD sugar-binding motifPFAMPF04488Gly_transf_sugcoord: 49..127
e-value: 1.3E-13
score: 51.4
NoneNo IPR availableGENE3DG3DSA:3.90.550.20coord: 31..278
e-value: 2.6E-15
score: 59.3
NoneNo IPR availablePANTHERPTHR32385FAMILY NOT NAMEDcoord: 27..223
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILYSSF53448Nucleotide-diphospho-sugar transferasescoord: 28..230