SMED30033755

Overview
NameSMED30033755
Smed IDSMED30033755
Length (bp)2519
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30033755 (SMED30033755) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30033755 (SMED30033755) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30033755 (SMED30033755) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30033755 (SMED30033755) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30033755

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 17

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30033755SMESG000014563.1 SMESG000014554.1 SMESG000014542.1 Contig4736uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30033755SMESG000014563.1 SMESG000014554.1 SMESG000014542.1 Contig4736newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30033755SMESG000017804.1 Contig4736uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30033755SMESG000017804.1 Contig4736newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pigment cellSMED30033755SMESG000014578.1 SMESG000014566.1 SMESG000014563.1 SMESG000014554.1 SMESG000014544.1 SMESG000014542.1 SmedASXL_017630SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017
whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30033755SMESG000014578.1 SMESG000014566.1 SMESG000014563.1 SMESG000014562.1 SMESG000014554.1 SMESG000014553.1 SMESG000014544.1 SMESG000014542.1 SMESG000014539.1 Contig33927uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30033755SMESG000014578.1 SMESG000014566.1 SMESG000014563.1 SMESG000014562.1 SMESG000014554.1 SMESG000014553.1 SMESG000014544.1 SMESG000014542.1 SMESG000014539.1 Contig33927newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30033755SMESG000014578.1 SMESG000014566.1 SMESG000014563.1 SMESG000014554.1 SMESG000014544.1 SMESG000014542.1 SMESG000014539.1 Contig42720newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30033755SMESG000014578.1 SMESG000014566.1 SMESG000014563.1 SMESG000014554.1 SMESG000014544.1 SMESG000014542.1 SMESG000014539.1 Contig42720uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30033755SMESG000066562.1 SMESG000036854.1 SMESG000014563.1 SMESG000014544.1 SMESG000014542.1 SMESG000014539.1 Contig29967uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30033755SMESG000066562.1 SMESG000036854.1 SMESG000014563.1 SMESG000014544.1 SMESG000014542.1 SMESG000014539.1 Contig29967newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30033755SMESG000014563.1 SMESG000014554.1 SMESG000014542.1 Contig4736uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30033755SMESG000014563.1 SMESG000014554.1 SMESG000014542.1 Contig4736newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30033755SMESG000017804.1 Contig4736uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30033755SMESG000017804.1 Contig4736newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pigment cellSMED30033755SMESG000070072.1 dd_Smed_v6_1789_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30033755SMESG000070072.1 dd_Smed_v6_1789_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30033755 vs. Planmine SMEST
Match: SMESG000014542.1 (SMESG000014542.1)

HSP 1 Score: 900.582 bits (2326), Expect = 0.000e+0
Identity = 457/542 (84.32%), Postives = 483/542 (89.11%), Query Frame = 1
Query:  103 IMLLNAVIFCLIQQLVLTDAQPLLTVCFVSTVPHLLKNTFDIQIKPQANGTKLSDDPSDTMVIRIYSFNFTEEEKSILNYGSQDAIDEEILGKFMDIKVWEKNFSSLRINISIMFKRGAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVNRKEIIINDMVMASCRAFGGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFXXXXXXXXXXXXXXXXXXXXLQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAIT 1728
            I +L  ++F L   L+  +A+  L V  VS  PH +   F + I+ ++   KLS DPSDTMVIRI   NFTE E++ L YG+   +D+E  G  MDIK+WEKN  S +I+ISI+FKR AHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKV+RKEIIINDMVMASCRAF GIPQEKPRFEDHAQLNVTEIIYNETANEISMNLR SLDDHLTNFSCSAGNVTETFIIRIHHHPSKIHI PDIKDILQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEI+IRSSAPTGSAWMFKCLMKSTIA VAMTKEKNI FRIASHPTSILI PVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNA+ FYNIP KGDQI+INSNS  GSKWT +CTMKS LVNT K LNKTISF+I 
Sbjct:    3 IQMLTVLLFIL--HLLFAEAEIALFVRSVSNFPHFIGEAFSLDIQSRSENGKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVDRKEIIINDMVMASCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRASLDDHLTNFSCSAGNVTETFIIRIHHHPSKIHITPDIKDILQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIFIRSSAPTGSAWMFKCLMKSTIAEVAMTKEKNITFRIASHPTSILIRPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIA 542          

HSP 2 Score: 162.155 bits (409), Expect = 6.672e-41
Identity = 80/198 (40.40%), Postives = 116/198 (58.59%), Query Frame = 1
Query: 1138 RIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1731
            RI  HP+ I I P    I++ N  I C   DGYP     +++I G  +AS YY   +NG++I ++ + PT S W  KC +KS +  V +   + I+FRIA+HPT++ I+P+       NSTI C   DGYP     W++I G  +A+L+YNI   G QI++  N  T S+W  +C++KS L N   ILN+TISF I +
Sbjct:  249 RIHHHPSKIHITPDIKDILQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIFIRSSAPTGSAWMFKCLMKSTIAEVAMTKEKNITFRIASHPTSILIRPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIAN 446          

HSP 3 Score: 154.066 bits (388), Expect = 2.668e-38
Identity = 74/185 (40.00%), Postives = 108/185 (58.38%), Query Frame = 1
Query:  901 LQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTAL 1455
            ++ N  I C   DGYP     +++I G  +AS YY   +NG++I+++ + PT S W  KC +KS +  V +   + I+FRIA+HPT++ I P+       NSTI C   DGYP     W++I G  +A  +YNI   G QIH+  N P  S+W +KC++KS L N    LN+TISF IA +P  +
Sbjct:  364 MEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIAKNPMEI 548          

HSP 4 Score: 102.834 bits (255), Expect = 3.866e-22
Identity = 59/176 (33.52%), Postives = 90/176 (51.14%), Query Frame = 1
Query: 1213 ACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENV-CVILNRTISF--RIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1731
            +C    G P  +  +E  A L    + YN   N   ++L+             S+   L N  C   N T +F  RI +HP+ ++I P IK   ++N  I+C+ +DGYP P +++QLI+GP NA+ +Y     G +I+I S++ TGS W F+C MKS +        K I+F I S
Sbjct:  186 SCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLR------------ASLDDHLTNFSCSAGNVTETFIIRIHHHPSKIHITPDIKDILQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIFIRSSAPTGSAWMFKCLMKSTIAEVAMTKEKNITFRIAS 349          

HSP 5 Score: 69.3218 bits (168), Expect = 9.747e-12
Identity = 37/60 (61.67%), Postives = 41/60 (68.33%), Query Frame = 3
Query: 1998 ILGNPMEICGEKCLNSRIFEIEETVKENVRLNEDIKLILN----QMEAMRKEHQEIRKAF 2165
            I  NPMEICGEKCLNSRI EIEE+V EN RL EDI+ ILN    QM AM K  Q+  +  
Sbjct:  541 IAKNPMEICGEKCLNSRISEIEESVDENERLQEDIRSILNQVKDQMIAMGKGQQKTSRGM 600          
BLAST of SMED30033755 vs. Planmine SMEST
Match: SMESG000014544.1 (SMESG000014544.1)

HSP 1 Score: 898.271 bits (2320), Expect = 0.000e+0
Identity = 454/542 (83.76%), Postives = 483/542 (89.11%), Query Frame = 1
Query:  103 IMLLNAVIFCLIQQLVLTDAQPLLTVCFVSTVPHLLKNTFDIQIKPQANGTKLSDDPSDTMVIRIYSFNFTEEEKSILNYGSQDAIDEEILGKFMDIKVWEKNFSSLRINISIMFKRGAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVNRKEIIINDMVMASCRAFGGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFXXXXXXXXXXXXXXXXXXXXLQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAIT 1728
            + +L  ++F L   L+  +A+  L V  VS  PH + + F + I+ ++  +KLS DPSDTMVIRI   NFTE E++ L YG+   +D+E  G  MDIK+WEKN  S +I+ISI+FKR AHNKYLEI YFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALT+LPIKV+RKEIIINDMVMASCRAF GIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFII IHHHPSKIHIKPDIKDILQANRAIQCY QDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAP GSAWMFKCLMKSTIAGVAMTKEKNI FRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAG ESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPT LYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNA+ FYNIP KGDQI+INSNS  GSKWT +CTMKS LVNT K LNKTISF+I 
Sbjct:    3 MQMLTVLLFIL--HLLFAEAEIALFVRSVSNFPHFIGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEILYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTVLPIKVDRKEIIINDMVMASCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPNGSAWMFKCLMKSTIAGVAMTKEKNITFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTTLYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIA 542          

HSP 2 Score: 162.155 bits (409), Expect = 6.249e-41
Identity = 79/197 (40.10%), Postives = 115/197 (58.38%), Query Frame = 1
Query: 1141 IASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1731
            I  HP+ I I P    I++ N  I C   DGYP     +++I G  +AS YY   +NG++I+++ + P  S W  KC +KS +  V +   + I+FRIA+HPT++ I P+       NSTI C   DGYP     W++I GP +A+L+YNI   G QI++  N  T S+W  +C++KS L N   ILN+TISF I +
Sbjct:  250 IHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPNGSAWMFKCLMKSTIAGVAMTKEKNITFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIAN 446          

HSP 3 Score: 157.918 bits (398), Expect = 1.633e-39
Identity = 75/185 (40.54%), Postives = 109/185 (58.92%), Query Frame = 1
Query:  901 LQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTAL 1455
            ++ N  I C   DGYP     +++I GP +AS YY   +NG++I+++ + PT S W  KC +KS +  V +   + I+FRIA+HPT++ I P+       NSTI C   DGYP     W++I G  +A  +YNI   G QIH+  N P  S+W +KC++KS L N    LN+TISF IA +P  +
Sbjct:  364 MEPNSTIACGPADGYPVARATWEVIAGPESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTTLYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIAKNPMEI 548          

HSP 4 Score: 98.9821 bits (245), Expect = 5.183e-21
Identity = 58/176 (32.95%), Postives = 89/176 (50.57%), Query Frame = 1
Query: 1213 ACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENV-CVILNRTISFRIA--NHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1731
            +C    G P  +  +E  A L    + YN   N   ++L+             S+   L N  C   N T +F I   +HP+ ++I+P IK   ++N  I+C+ +DGYP P +++QLI+GP NA+ +Y     G +IYI S++  GS W F+C MKS +        K I+F I S
Sbjct:  186 SCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLR------------TSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPNGSAWMFKCLMKSTIAGVAMTKEKNITFRIAS 349          

HSP 5 Score: 72.0182 bits (175), Expect = 1.331e-12
Identity = 38/60 (63.33%), Postives = 42/60 (70.00%), Query Frame = 3
Query: 1998 ILGNPMEICGEKCLNSRIFEIEETVKENVRLNEDIKLILN----QMEAMRKEHQEIRKAF 2165
            I  NPMEICGEKCLNSRI EIEE+V EN RL +DI+ ILN    QM AM KE QE  +  
Sbjct:  541 IAKNPMEICGEKCLNSRISEIEESVDENERLQKDIRSILNQVKEQMIAMGKEQQETSRGL 600          
BLAST of SMED30033755 vs. Planmine SMEST
Match: SMESG000014544.1 (SMESG000014544.1)

HSP 1 Score: 898.271 bits (2320), Expect = 0.000e+0
Identity = 454/542 (83.76%), Postives = 483/542 (89.11%), Query Frame = 1
Query:  103 IMLLNAVIFCLIQQLVLTDAQPLLTVCFVSTVPHLLKNTFDIQIKPQANGTKLSDDPSDTMVIRIYSFNFTEEEKSILNYGSQDAIDEEILGKFMDIKVWEKNFSSLRINISIMFKRGAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVNRKEIIINDMVMASCRAFGGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFXXXXXXXXXXXXXXXXXXXXLQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAIT 1728
            + +L  ++F L   L+  +A+  L V  VS  PH + + F + I+ ++  +KLS DPSDTMVIRI   NFTE E++ L YG+   +D+E  G  MDIK+WEKN  S +I+ISI+FKR AHNKYLEI YFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALT+LPIKV+RKEIIINDMVMASCRAF GIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFII IHHHPSKIHIKPDIKDILQANRAIQCY QDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAP GSAWMFKCLMKSTIAGVAMTKEKNI FRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAG ESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPT LYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNA+ FYNIP KGDQI+INSNS  GSKWT +CTMKS LVNT K LNKTISF+I 
Sbjct:    1 MQMLTVLLFIL--HLLFAEAEIALFVRSVSNFPHFIGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEILYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTVLPIKVDRKEIIINDMVMASCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPNGSAWMFKCLMKSTIAGVAMTKEKNITFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTTLYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIA 540          

HSP 2 Score: 162.155 bits (409), Expect = 5.971e-41
Identity = 79/197 (40.10%), Postives = 115/197 (58.38%), Query Frame = 1
Query: 1141 IASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1731
            I  HP+ I I P    I++ N  I C   DGYP     +++I G  +AS YY   +NG++I+++ + P  S W  KC +KS +  V +   + I+FRIA+HPT++ I P+       NSTI C   DGYP     W++I GP +A+L+YNI   G QI++  N  T S+W  +C++KS L N   ILN+TISF I +
Sbjct:  248 IHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPNGSAWMFKCLMKSTIAGVAMTKEKNITFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIAN 444          

HSP 3 Score: 157.918 bits (398), Expect = 1.576e-39
Identity = 75/185 (40.54%), Postives = 109/185 (58.92%), Query Frame = 1
Query:  901 LQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTAL 1455
            ++ N  I C   DGYP     +++I GP +AS YY   +NG++I+++ + PT S W  KC +KS +  V +   + I+FRIA+HPT++ I P+       NSTI C   DGYP     W++I G  +A  +YNI   G QIH+  N P  S+W +KC++KS L N    LN+TISF IA +P  +
Sbjct:  362 MEPNSTIACGPADGYPVARATWEVIAGPESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTTLYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIAKNPMEI 546          

HSP 4 Score: 98.9821 bits (245), Expect = 5.125e-21
Identity = 58/176 (32.95%), Postives = 89/176 (50.57%), Query Frame = 1
Query: 1213 ACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENV-CVILNRTISFRIA--NHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1731
            +C    G P  +  +E  A L    + YN   N   ++L+             S+   L N  C   N T +F I   +HP+ ++I+P IK   ++N  I+C+ +DGYP P +++QLI+GP NA+ +Y     G +IYI S++  GS W F+C MKS +        K I+F I S
Sbjct:  184 SCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLR------------TSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPNGSAWMFKCLMKSTIAGVAMTKEKNITFRIAS 347          

HSP 5 Score: 72.0182 bits (175), Expect = 1.352e-12
Identity = 38/60 (63.33%), Postives = 42/60 (70.00%), Query Frame = 3
Query: 1998 ILGNPMEICGEKCLNSRIFEIEETVKENVRLNEDIKLILN----QMEAMRKEHQEIRKAF 2165
            I  NPMEICGEKCLNSRI EIEE+V EN RL +DI+ ILN    QM AM KE QE  +  
Sbjct:  539 IAKNPMEICGEKCLNSRISEIEESVDENERLQKDIRSILNQVKEQMIAMGKEQQETSRGL 598          
BLAST of SMED30033755 vs. Planmine SMEST
Match: SMESG000014544.1 (SMESG000014544.1)

HSP 1 Score: 898.271 bits (2320), Expect = 0.000e+0
Identity = 454/540 (84.07%), Postives = 482/540 (89.26%), Query Frame = 1
Query:  109 LLNAVIFCLIQQLVLTDAQPLLTVCFVSTVPHLLKNTFDIQIKPQANGTKLSDDPSDTMVIRIYSFNFTEEEKSILNYGSQDAIDEEILGKFMDIKVWEKNFSSLRINISIMFKRGAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVNRKEIIINDMVMASCRAFGGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFXXXXXXXXXXXXXXXXXXXXLQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAIT 1728
            +L  ++F L   L+  +A+  L V  VS  PH + + F + I+ ++  +KLS DPSDTMVIRI   NFTE E++ L YG+   +D+E  G  MDIK+WEKN  S +I+ISI+FKR AHNKYLEI YFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALT+LPIKV+RKEIIINDMVMASCRAF GIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFII IHHHPSKIHIKPDIKDILQANRAIQCY QDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAP GSAWMFKCLMKSTIAGVAMTKEKNI FRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAG ESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPT LYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNA+ FYNIP KGDQI+INSNS  GSKWT +CTMKS LVNT K LNKTISF+I 
Sbjct:    1 MLTVLLFIL--HLLFAEAEIALFVRSVSNFPHFIGDAFSLDIQSRSENSKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEILYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTVLPIKVDRKEIIINDMVMASCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPNGSAWMFKCLMKSTIAGVAMTKEKNITFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTTLYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIA 538          

HSP 2 Score: 162.155 bits (409), Expect = 5.455e-41
Identity = 79/197 (40.10%), Postives = 115/197 (58.38%), Query Frame = 1
Query: 1141 IASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1731
            I  HP+ I I P    I++ N  I C   DGYP     +++I G  +AS YY   +NG++I+++ + P  S W  KC +KS +  V +   + I+FRIA+HPT++ I P+       NSTI C   DGYP     W++I GP +A+L+YNI   G QI++  N  T S+W  +C++KS L N   ILN+TISF I +
Sbjct:  246 IHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPNGSAWMFKCLMKSTIAGVAMTKEKNITFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGPESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIAN 442          

HSP 3 Score: 157.918 bits (398), Expect = 1.521e-39
Identity = 75/185 (40.54%), Postives = 109/185 (58.92%), Query Frame = 1
Query:  901 LQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTAL 1455
            ++ N  I C   DGYP     +++I GP +AS YY   +NG++I+++ + PT S W  KC +KS +  V +   + I+FRIA+HPT++ I P+       NSTI C   DGYP     W++I G  +A  +YNI   G QIH+  N P  S+W +KC++KS L N    LN+TISF IA +P  +
Sbjct:  360 MEPNSTIACGPADGYPVARATWEVIAGPESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTTLYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIAKNPMEI 544          

HSP 4 Score: 99.3673 bits (246), Expect = 4.854e-21
Identity = 58/176 (32.95%), Postives = 89/176 (50.57%), Query Frame = 1
Query: 1213 ACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENV-CVILNRTISFRIA--NHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1731
            +C    G P  +  +E  A L    + YN   N   ++L+             S+   L N  C   N T +F I   +HP+ ++I+P IK   ++N  I+C+ +DGYP P +++QLI+GP NA+ +Y     G +IYI S++  GS W F+C MKS +        K I+F I S
Sbjct:  182 SCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLR------------TSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPNGSAWMFKCLMKSTIAGVAMTKEKNITFRIAS 345          

HSP 5 Score: 72.0182 bits (175), Expect = 1.339e-12
Identity = 38/60 (63.33%), Postives = 42/60 (70.00%), Query Frame = 3
Query: 1998 ILGNPMEICGEKCLNSRIFEIEETVKENVRLNEDIKLILN----QMEAMRKEHQEIRKAF 2165
            I  NPMEICGEKCLNSRI EIEE+V EN RL +DI+ ILN    QM AM KE QE  +  
Sbjct:  537 IAKNPMEICGEKCLNSRISEIEESVDENERLQKDIRSILNQVKEQMIAMGKEQQETSRGL 596          
BLAST of SMED30033755 vs. Planmine SMEST
Match: SMESG000014542.1 (SMESG000014542.1)

HSP 1 Score: 898.271 bits (2320), Expect = 0.000e+0
Identity = 456/540 (84.44%), Postives = 482/540 (89.26%), Query Frame = 1
Query:  109 LLNAVIFCLIQQLVLTDAQPLLTVCFVSTVPHLLKNTFDIQIKPQANGTKLSDDPSDTMVIRIYSFNFTEEEKSILNYGSQDAIDEEILGKFMDIKVWEKNFSSLRINISIMFKRGAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVNRKEIIINDMVMASCRAFGGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFXXXXXXXXXXXXXXXXXXXXLQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAIT 1728
            +L  ++F L   L+  +A+  L V  VS  PH +   F + I+ ++   KLS DPSDTMVIRI   NFTE E++ L YG+   +D+E  G  MDIK+WEKN  S +I+ISI+FKR AHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKV+RKEIIINDMVMASCRAF GIPQEKPRFEDHAQLNVTEIIYNETANEISMNLR SLDDHLTNFSCSAGNVTETFIIRIHHHPSKIHI PDIKDILQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEI+IRSSAPTGSAWMFKCLMKSTIA VAMTKEKNI FRIASHPTSILI PVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNA+ FYNIP KGDQI+INSNS  GSKWT +CTMKS LVNT K LNKTISF+I 
Sbjct:    1 MLTVLLFIL--HLLFAEAEIALFVRSVSNFPHFIGEAFSLDIQSRSENGKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLPIKVDRKEIIINDMVMASCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLRASLDDHLTNFSCSAGNVTETFIIRIHHHPSKIHITPDIKDILQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIFIRSSAPTGSAWMFKCLMKSTIAEVAMTKEKNITFRIASHPTSILIRPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIA 538          

HSP 2 Score: 161.77 bits (408), Expect = 8.186e-41
Identity = 80/198 (40.40%), Postives = 116/198 (58.59%), Query Frame = 1
Query: 1138 RIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1731
            RI  HP+ I I P    I++ N  I C   DGYP     +++I G  +AS YY   +NG++I ++ + PT S W  KC +KS +  V +   + I+FRIA+HPT++ I+P+       NSTI C   DGYP     W++I G  +A+L+YNI   G QI++  N  T S+W  +C++KS L N   ILN+TISF I +
Sbjct:  245 RIHHHPSKIHITPDIKDILQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIFIRSSAPTGSAWMFKCLMKSTIAEVAMTKEKNITFRIASHPTSILIRPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIAN 442          

HSP 3 Score: 153.68 bits (387), Expect = 3.163e-38
Identity = 74/185 (40.00%), Postives = 108/185 (58.38%), Query Frame = 1
Query:  901 LQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHPTSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTAL 1455
            ++ N  I C   DGYP     +++I G  +AS YY   +NG++I+++ + PT S W  KC +KS +  V +   + I+FRIA+HPT++ I P+       NSTI C   DGYP     W++I G  +A  +YNI   G QIH+  N P  S+W +KC++KS L N    LN+TISF IA +P  +
Sbjct:  360 MEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKCTMKSVLVNTKKSLNKTISFSIAKNPMEI 544          

HSP 4 Score: 102.449 bits (254), Expect = 4.568e-22
Identity = 59/176 (33.52%), Postives = 90/176 (51.14%), Query Frame = 1
Query: 1213 ACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWEIKCSVKSCLENV-CVILNRTISF--RIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITS 1731
            +C    G P  +  +E  A L    + YN   N   ++L+             S+   L N  C   N T +F  RI +HP+ ++I P IK   ++N  I+C+ +DGYP P +++QLI+GP NA+ +Y     G +I+I S++ TGS W F+C MKS +        K I+F I S
Sbjct:  182 SCRAFSGIPQEKPRFEDHAQLNVTEIIYNETANEISMNLR------------ASLDDHLTNFSCSAGNVTETFIIRIHHHPSKIHITPDIKDILQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIFIRSSAPTGSAWMFKCLMKSTIAEVAMTKEKNITFRIAS 345          

HSP 5 Score: 69.3218 bits (168), Expect = 9.394e-12
Identity = 37/60 (61.67%), Postives = 41/60 (68.33%), Query Frame = 3
Query: 1998 ILGNPMEICGEKCLNSRIFEIEETVKENVRLNEDIKLILN----QMEAMRKEHQEIRKAF 2165
            I  NPMEICGEKCLNSRI EIEE+V EN RL EDI+ ILN    QM AM K  Q+  +  
Sbjct:  537 IAKNPMEICGEKCLNSRISEIEESVDENERLQEDIRSILNQVKDQMIAMGKGQQKTSRGM 596          
The following BLAST results are available for this feature:
BLAST of SMED30033755 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033755 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033755 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033755 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033755 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033755 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033755 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033755 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033755 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033755 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033755 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033755 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033755 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033755 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000014542.16.672e-4184.32SMESG000014542.1[more]
SMESG000014544.16.249e-4183.76SMESG000014544.1[more]
SMESG000014544.15.971e-4183.76SMESG000014544.1[more]
SMESG000014544.15.455e-4184.07SMESG000014544.1[more]
SMESG000014542.18.186e-4184.44SMESG000014542.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033755 ID=SMED30033755|Name=SMED30033755|organism=Schmidtea mediterranea sexual|type=transcript|length=2519bp
AGAAGTTTCATTGTCAATTTTATAGTGGTCTTGGACTTTGAACAGTCCCG
GTTCTTTATTCATATTTTTAAACTCTTAGTATTTCGGTGAACAATTCCTT
CAATAATGTTACTGAATGCTGTAATCTTTTGTCTCATACAACAATTGGTT
CTTACGGATGCGCAACCGCTACTGACTGTTTGTTTCGTTTCGACAGTTCC
ACATCTTCTTAAGAATACCTTTGATATCCAAATCAAGCCTCAAGCTAACG
GAACCAAATTGAGTGATGATCCGTCAGATACAATGGTCATACGAATTTAT
TCCTTTAATTTCACGGAGGAAGAAAAATCGATTCTAAATTATGGATCACA
AGATGCAATAGATGAAGAGATACTGGGTAAATTCATGGACATCAAGGTAT
GGGAGAAGAATTTCTCATCCTTGAGAATAAACATTTCAATAATGTTCAAA
CGAGGGGCCCATAACAAATATCTAGAAATATTTTACTTTTCTGCTCCCAA
TAAATTTGTACGAAGAAAATATTTGAAAAATCTCTCCCTTGCCAAACGGT
TATTTCATGAACTGATTGACTTGTTTATCCCATCCACATTGGCACTCACG
CTGCTCCCAATCAAGGTTAACAGAAAGGAAATAATAATAAATGATATGGT
AATGGCGTCCTGCAGAGCATTCGGTGGGATACCACAAGAAAAACCGAGAT
TCGAGGATCACGCTCAATTGAATGTGACGGAGATAATATATAATGAAACG
GCCAATGAGATCAGTATGAATTTGCGCACATCTCTCGATGACCATCTCAC
TAATTTCAGCTGCAGCGCCGGAAATGTTACGGAAACGTTCATAATCCGTA
TTCATCATCATCCGTCCAAGATTCACATAAAGCCAGATATTAAAGATATA
TTGCAAGCCAACAGAGCCATTCAATGCTATGTCCAAGATGGATACCCTCA
GCCTTCATTGCATTTCCAGCTCATCAAAGGTCCATCAAACGCATCTCACT
ATTATGAGACTAGGAAAAACGGATCCGAAATCTACATCCGTTCAAGTGCC
CCAACCGGATCAGCGTGGATGTTCAAATGCTTAATGAAAAGCACAATTGC
AGGGGTAGCAATGACCAAGGAAAAGAATATTAACTTTCGAATAGCCAGCC
ATCCGACATCAATCCTGATCATGCCAGTGCCAGATGGAATAATGGAACCG
AATTCAACAATTGCTTGTGGCCCAGCTGACGGATATCCTGTTGCCAGAGC
AACATGGGAAGTTATTGCGGGCCTAGAAAGCGCTTCACTATATTATAATA
TTATGCGCAATGGAACGCAAATTCATCTGAAAGAAAATTGCCCTACAAAA
TCTGAATGGGAAATCAAATGCTCTGTTAAAAGTTGCTTGGAAAATGTTTG
TGTAATCCTCAATCGGACAATTTCATTTAGAATAGCAAATCACCCGACCG
CATTATATATTCAACCAATCATCAAAAAGAGATTCCGATCAAACTCAACA
ATAAAATGCTTCACAAAAGATGGATATCCAGATCCAGAAATAAATTGGCA
ATTAATTGAAGGCCCCCCAAATGCTAACTTGTTTTACAATATTCCTCCAA
AGGGTGACCAAATCTACATCAATAGCAACAGCCAAACCGGTTCAAAATGG
ACGTTCAGATGCACAATGAAAAGCTTTCTCGTGAATACCAATAAAATTTT
GAATAAAACTATTTCATTTGCTATTACTAGTAAGCTTTTGATTGGATACT
ATTTCTTTGTTTTGATTGGGTAATATTTGTTGGTTTTGATTGGATATGAT
CTGGCGGTGGATATGATCATGATTAATATGGATACCCTTGTCATAATTAT
TTTAGGGAGCCCTCAATAATGAATTATCCACTCAACATGGTAAAATCCAA
ACTGCAAATATTATATATTCTTTTCGTTATTTCACTGTTACTTCTCTTTT
TTACTGATTATATTTTGAATTGATACTTTGCAAATTTATTTATTGTTATT
TTAGGGAATCCGATGGAAATTTGTGGGGAGAAATGTTTAAATTCACGGAT
TTTTGAAATTGAAGAAACGGTAAAGGAGAACGTGAGGCTAAATGAGGATA
TCAAGTTGATATTGAATCAAATGGAAGCCATGAGAAAGGAACACCAAGAA
ATAAGAAAGGCGTTTCTATATTTTCAATGGAATTTTAATACCACAACTTA
TGAAATACAAACAACCCAAACTTTTACTTCAGCGTTCCCATTGTCTGTTC
GATTCCAAACGGAATTCGTGGAAAAGGACCACAAACTATTCAAATGCGGA
TTGATAGTTAGGAATGACAATCCACAAATGTCTGTAAACTTGACAGTGAC
ATACGAATGTTGGATATCTGCAAAATCGTCGCCCCTCAAATTGATATTTA
ATGTTAAAGTCAAACCTCTAGAAGAAAATAAAGAAAAAAAAAATTTCTTC
ATATTTCCTTCTAGTAAGATATTTGGCCAAAACCTTTCTCAAACGAAACT
CTCTGTTGGATGTCATTTC
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protein sequence of SMED30033755-orf-1

>SMED30033755-orf-1 ID=SMED30033755-orf-1|Name=SMED30033755-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=556bp
MLLNAVIFCLIQQLVLTDAQPLLTVCFVSTVPHLLKNTFDIQIKPQANGT
KLSDDPSDTMVIRIYSFNFTEEEKSILNYGSQDAIDEEILGKFMDIKVWE
KNFSSLRINISIMFKRGAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLF
HELIDLFIPSTLALTLLPIKVNRKEIIINDMVMASCRAFGGIPQEKPRFE
DHAQLNVTEIIYNETANEISMNLRTSLDDHLTNFSCSAGNVTETFIIRIH
HHPSKIHIKPDIKDILQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYY
ETRKNGSEIYIRSSAPTGSAWMFKCLMKSTIAGVAMTKEKNINFRIASHP
TSILIMPVPDGIMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIM
RNGTQIHLKENCPTKSEWEIKCSVKSCLENVCVILNRTISFRIANHPTAL
YIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNANLFYNIPPKG
DQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITSKLLIGYYF
FVLIG*
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protein sequence of SMED30033755-orf-2

>SMED30033755-orf-2 ID=SMED30033755-orf-2|Name=SMED30033755-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=169bp
MEICGEKCLNSRIFEIEETVKENVRLNEDIKLILNQMEAMRKEHQEIRKA
FLYFQWNFNTTTYEIQTTQTFTSAFPLSVRFQTEFVEKDHKLFKCGLIVR
NDNPQMSVNLTVTYECWISAKSSPLKLIFNVKVKPLEENKEKKNFFIFPS
SKIFGQNLSQTKLSVGCHF
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR007110Ig-like_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding