SMED30033696

Overview
NameSMED30033696
Smed IDSMED30033696
Length (bp)733
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30033696 (SMED30033696) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30033696 (SMED30033696) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30033696

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
testis primordiumSMED30033696SMESG000029360.1 Contig3193_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism juvenile cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30033696 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000080:202326..212684 -3049
v31.000509:14822..15639 +2688
v31.000509:115885..116702 +2681
v31.000080:150105..150875 -2297
Homology
BLAST of SMED30033696 vs. Planmine SMEST
Match: SMESG000029360.1 (SMESG000029360.1)

HSP 1 Score: 319.316 bits (817), Expect = 7.028e-112
Identity = 168/174 (96.55%), Postives = 171/174 (98.28%), Query Frame = 2
Query:   92 MSAIQNXXXXXXXXXXXXXSAPHYEKKITTLNCLDGAKITTSCIFAIEIKPIITTPLSCILEFDSRSAQMILTIFNIKTKSEIDRKCPKFTMRVMTLGKSEMISRHCGEEIVGVNKTVEGFTSAYINYDNQMLNDTTSNAFIKISKTNLEKQNCEKGKSYQSRFDPHLSYVATL 613
            MSAIQNLALVLTLGLNLV SAP++EKKITTLNCL+GAKITTSCIFAIEIKPIITTPLSCILEFDSRSAQMILTIFNIKTKSEIDRKCPKFTM VMTLGK EMISRHCGEEIVGVNKTVEGFTSAYINYDNQMLNDTTSNAFIKISKTNLEKQNCEKGKSYQSRFDPHLSYVATL
Sbjct:    1 MSAIQNLALVLTLGLNLVASAPYFEKKITTLNCLNGAKITTSCIFAIEIKPIITTPLSCILEFDSRSAQMILTIFNIKTKSEIDRKCPKFTMHVMTLGKYEMISRHCGEEIVGVNKTVEGFTSAYINYDNQMLNDTTSNAFIKISKTNLEKQNCEKGKSYQSRFDPHLSYVATL 174          
BLAST of SMED30033696 vs. Planmine SMEST
Match: SMESG000029364.1 (SMESG000029364.1)

HSP 1 Score: 238.039 bits (606), Expect = 1.002e-79
Identity = 131/174 (75.29%), Postives = 145/174 (83.33%), Query Frame = 2
Query:   92 MSAIQNXXXXXXXXXXXXXSAPHYEKKITTLNCLDGAKITTSCIFAIEIKPIITTPLSCILEFDSRSAQMILTIFNIKTKSEIDRKCPKFTMRVMTLGKSEMISRHCGEEIVGVNKTVEGFTSAYINYDNQMLNDTTSNAFIKISKTNLEKQNCEKGKSYQSRFDPHLSYVATL 613
            MSAIQNLALVLTLGLNLVVSAPHYEKKITTLNCLDGAKITTSCIFAIEI P+I+ PLSC L+F S SAQ+ILT FNI  KS+ID KCP F M V + GK++ IS  CG EI G+ KTVEGFTSA +NYDN+ L D+T+ AFIKISKTNLEKQNCEKGKSYQ +FD  LS VA L
Sbjct:    1 MSAIQNLALVLTLGLNLVVSAPHYEKKITTLNCLDGAKITTSCIFAIEINPVISAPLSCTLQFISSSAQVILTAFNITIKSQIDNKCPMFNMNVFSSGKTQKISTTCGGEISGIKKTVEGFTSAVLNYDNKGLTDSTTVAFIKISKTNLEKQNCEKGKSYQVKFDALLSSVAIL 174          
BLAST of SMED30033696 vs. Planmine SMEST
Match: SMESG000029371.1 (SMESG000029371.1)

HSP 1 Score: 194.126 bits (492), Expect = 1.921e-62
Identity = 108/174 (62.07%), Postives = 127/174 (72.99%), Query Frame = 2
Query:   92 MSAIQNXXXXXXXXXXXXXSAPHYEKKITTLNCLDGAKITTSCIFAIEIKPIITTPLSCILEFDSRSAQMILTIFNIKTKSEIDRKCPKFTMRVMTLGKSEMISRHCGEEIVGVNKTVEGFTSAYINYDNQMLNDTTSNAFIKISKTNLEKQNCEKGKSYQSRFDPHLSYVATL 613
            MSAIQNLAL+LT  L LV SA + E+K TTLNCL+G  I TSCIF+IEIKP IT PL C LEFDS +A+++LT+ NI    ++D+ CPKF   V + G  E IS  CG+    +NKTVEGFTSA I YDN+ LNDTT+ AFIKISKTNL  +NC   KSYQ RFD HLSYVA L
Sbjct:    1 MSAIQNLALILTFELKLVASAQYNEQKTTTLNCLNGDLILTSCIFSIEIKPAITAPLICTLEFDSNNAKVLLTVTNITVTGQVDKICPKFNFEVFSSGNPEKISTTCGKSTPLINKTVEGFTSAIIFYDNKGLNDTTTVAFIKISKTNLGNENCNTDKSYQLRFDVHLSYVAIL 174          
BLAST of SMED30033696 vs. Planmine SMEST
Match: SMESG000029371.1 (SMESG000029371.1)

HSP 1 Score: 168.318 bits (425), Expect = 1.378e-52
Identity = 95/157 (60.51%), Postives = 114/157 (72.61%), Query Frame = 2
Query:   92 MSAIQNXXXXXXXXXXXXXSAPHYEKKITTLNCLDGAKITTSCIFAIEIKPIITTPLSCILEFDSRSAQMILTIFNIKTKSEIDRKCPKFTMRVMTLGKSEMISRHCGEEIVGVNKTVEGFTSAYINYDNQMLNDTTSNAFIKISKTNLEKQNCEKG 562
            MSAIQNLAL+LT  L LV SA + E+K TTLNCL+G  I TSCIF+IEIKP IT PL C LEFDS +A+++LT+ NI    ++D+ CPKF   V + G  E IS  CG+    +NKTVEGFTSA I YDN+ LNDTT+ AFIKISKTNL  +NC  G
Sbjct:    1 MSAIQNLALILTFELKLVASAQYNEQKTTTLNCLNGDLILTSCIFSIEIKPAITAPLICTLEFDSNNAKVLLTVTNITVTGQVDKICPKFNFEVFSSGNPEKISTTCGKSTPLINKTVEGFTSAIIFYDNKGLNDTTTVAFIKISKTNLGNENCNTG 157          
BLAST of SMED30033696 vs. Planmine SMEST
Match: SMESG000029374.1 (SMESG000029374.1)

HSP 1 Score: 148.288 bits (373), Expect = 3.218e-44
Identity = 88/155 (56.77%), Postives = 106/155 (68.39%), Query Frame = 2
Query:   92 MSAIQNXXXXXXXXXXXXXSAPHYEKKITTLNCLDGAKITTSCIFAIEIKPIITTPLSCILEFDSRSAQMILTIFNIKTKSEIDRKCPKFTMRVMTLGKSEMISRHCGEEIVGVNKTVEGFTSAYINYDNQMLNDTTSNAFIKISKTNLEKQNCE 556
            MSAIQNLAL+LT GL LV SA + EKKITTLNCL+GAKITTSCIFAIEIKP+IT PL C L FDS +A+++LT+ NI    ++  KCPK    V   G  E IS  CG+    +NKT +GF+ A I YDN+ LNDT +  FI IS  N    N +
Sbjct:    1 MSAIQNLALILTFGLKLVASAKYNEKKITTLNCLNGAKITTSCIFAIEIKPVITAPLICNLLFDSNNAKVLLTVTNITITGQVGNKCPKINFAVFFSGNPEQISTTCGKSTPLINKTFDGFSRAIIYYDNKGLNDTNTVVFITISNQNCNTANAK 155          
The following BLAST results are available for this feature:
BLAST of SMED30033696 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033696 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033696 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033696 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033696 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033696 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033696 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033696 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033696 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033696 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033696 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033696 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033696 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033696 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000029360.17.028e-11296.55SMESG000029360.1[more]
SMESG000029364.11.002e-7975.29SMESG000029364.1[more]
SMESG000029371.11.921e-6262.07SMESG000029371.1[more]
SMESG000029371.11.378e-5260.51SMESG000029371.1[more]
SMESG000029374.13.218e-4456.77SMESG000029374.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033696 ID=SMED30033696|Name=SMED30033696|organism=Schmidtea mediterranea sexual|type=transcript|length=733bp
TTTCGAATATCAAACGAATATTTAAATTATTAGCTAATTATATACTAATC
AACCGATTATATTTATTATTGCAGTGTTGGATATTTTCTGCATGTCAGCT
ATTCAGAATTTGGCATTGGTTTTGACTCTGGGATTGAATTTGGTGGTCTC
TGCTCCACATTATGAGAAAAAAATCACCACTTTGAATTGTCTGGATGGCG
CTAAGATAACGACTTCATGCATATTTGCAATAGAAATCAAGCCCATAATT
ACTACTCCATTGTCTTGTATTCTCGAATTCGATTCGAGAAGTGCGCAAAT
GATTTTGACAATTTTTAACATCAAAACTAAAAGTGAAATTGATAGAAAAT
GTCCTAAATTCACCATGCGTGTGATGACGTTGGGAAAATCTGAAATGATT
TCAAGACATTGTGGTGAAGAAATTGTTGGGGTTAACAAAACAGTTGAAGG
ATTTACTAGTGCATATATTAACTACGATAATCAAATGTTGAATGATACAA
CTAGCAATGCCTTCATAAAGATTTCAAAGACCAATTTAGAAAAGCAAAAT
TGTGAAAAAGGTAAATCGTATCAATCGAGATTTGATCCTCACCTCAGTTA
TGTCGCCACTTTGATCAGCTTCATGATTTTTGCGAAAATTATTTGAATTT
GAATGTTTAATATTTACCTGTAAATAAGTCGCTATAAAATTCGAATATTA
TAATTATTATAACAAATTATAAGCTGCCATCCT
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protein sequence of SMED30033696-orf-1

>SMED30033696-orf-1 ID=SMED30033696-orf-1|Name=SMED30033696-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=185bp
MSAIQNLALVLTLGLNLVVSAPHYEKKITTLNCLDGAKITTSCIFAIEIK
PIITTPLSCILEFDSRSAQMILTIFNIKTKSEIDRKCPKFTMRVMTLGKS
EMISRHCGEEIVGVNKTVEGFTSAYINYDNQMLNDTTSNAFIKISKTNLE
KQNCEKGKSYQSRFDPHLSYVATLISFMIFAKII*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003304testis primordium
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..20
score: 0.696