Alpha-latrocrustotoxin-Lt1a
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30033645 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Human
Match: ANKRD42 (ankyrin repeat domain 42 [Source:HGNC Symbol;Acc:HGNC:26752]) HSP 1 Score: 176.792 bits (447), Expect = 1.133e-47 Identity = 112/342 (32.75%), Postives = 181/342 (52.92%), Query Frame = 1
Query: 4 SMLNDEYLEESEKRRLLFAYITADDDARFKRAVKDIDPVEIRKIIMEDTDL----SLLHWAALKGSVVIVNFLIDKMEFPSDLDN-NAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
S + E ++++ F I DA VK + + +R + + D+ + LHWAA GS+ +++L + +D+ + WTA+H+A I G+DAC+++LIM + + D RGCT LHLAA HG + + L +D + +W P+ YAA+HGR+ ++LL++ G + + D +G P+HLAA EGHL K FL + L A ++NG L LA+ K I+++I+ E + + E + L +PGH+AA GDL ++ L+E G I +N+ + G T +HKA GHI+ ++WL+ GA I E D+
Sbjct: 10 STSSRETANPCSRKKVHFGSI---HDAVRAGDVKQLSEIVVRGASINELDVLHKFTPLHWAAHSGSLECLHWL---LWHGADITHVTTRGWTASHIAAIRGQDACVQALIMNGANLTAQ-DDRGCTPLHLAATHGHSFTLQIM-----LRSGVDPSVTDKREWRPVHYAAFHGRLGCLQLLVKWGCSIEDVDYNGNLPVHLAAMEGHLHCFK-FLVSRMSSATQVLKAFNDNGENVLDLAQRFFKQNILQFIQGAEYEGKDLEDQETLAFPGHVAAFKGDLGMLKKLVEDGVININERADNGSTPMHKAAGQGHIECLQWLIKMGADSNITNKAGERPSDVA 338
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Human
Match: ANKRD42 (ankyrin repeat domain 42 [Source:HGNC Symbol;Acc:HGNC:26752]) HSP 1 Score: 176.407 bits (446), Expect = 2.291e-47 Identity = 112/342 (32.75%), Postives = 181/342 (52.92%), Query Frame = 1
Query: 4 SMLNDEYLEESEKRRLLFAYITADDDARFKRAVKDIDPVEIRKIIMEDTDL----SLLHWAALKGSVVIVNFLIDKMEFPSDLDN-NAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
S + E ++++ F I DA VK + + +R + + D+ + LHWAA GS+ +++L + +D+ + WTA+H+A I G+DAC+++LIM + + D RGCT LHLAA HG + + L +D + +W P+ YAA+HGR+ ++LL++ G + + D +G P+HLAA EGHL K FL + L A ++NG L LA+ K I+++I+ E + + E + L +PGH+AA GDL ++ L+E G I +N+ + G T +HKA GHI+ ++WL+ GA I E D+
Sbjct: 10 STSSRETANPCSRKKVHFGSI---HDAVRAGDVKQLSEIVVRGASINELDVLHKFTPLHWAAHSGSLECLHWL---LWHGADITHVTTRGWTASHIAAIRGQDACVQALIMNGANLTAQ-DDRGCTPLHLAATHGHSFTLQIM-----LRSGVDPSVTDKREWRPVHYAAFHGRLGCLQLLVKWGCSIEDVDYNGNLPVHLAAMEGHLHCFK-FLVSRMSSATQVLKAFNDNGENVLDLAQRFFKQNILQFIQGAEYEGKDLEDQETLAFPGHVAAFKGDLGMLKKLVEDGVININERADNGSTPMHKAAGQGHIECLQWLIKMGADSNITNKAGERPSDVA 338
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Human
Match: ANKRD42 (ankyrin repeat domain 42 [Source:HGNC Symbol;Acc:HGNC:26752]) HSP 1 Score: 175.637 bits (444), Expect = 4.379e-47 Identity = 111/341 (32.55%), Postives = 179/341 (52.49%), Query Frame = 1
Query: 4 SMLNDEYLEESEKRRLLFAYITADDDARFKRAVKDIDPVEIRKIIMEDTDL----SLLHWAALKGSVVIVNFLIDKMEFPSDLDN-NAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
S + E ++++ F I DA VK + + +R + + D+ + LHWAA GS+ +++L + +D+ + WTA+H+A I G+DAC+++LIM + + D RGCT LHLAA HG + + L + +W P+ YAA+HGR+ ++LL++ G + + D +G P+HLAA EGHL K FL + L A ++NG L LA+ K I+++I+ E + + E + L +PGH+AA GDL ++ L+E G I +N+ + G T +HKA GHI+ ++WL+ GA I E D+
Sbjct: 10 STSSRETANPCSRKKVHFGSI---HDAVRAGDVKQLSEIVVRGASINELDVLHKFTPLHWAAHSGSLECLHWL---LWHGADITHVTTRGWTASHIAAIRGQDACVQALIMNGANLTAQ-DDRGCTPLHLAATHGHSFTLQIM-----LRSGDPSVTDKREWRPVHYAAFHGRLGCLQLLVKWGCSIEDVDYNGNLPVHLAAMEGHLHCFK-FLVSRMSSATQVLKAFNDNGENVLDLAQRFFKQNILQFIQGAEYEGKDLEDQETLAFPGHVAAFKGDLGMLKKLVEDGVININERADNGSTPMHKAAGQGHIECLQWLIKMGADSNITNKAGERPSDVA 337
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Human
Match: ANKRD42 (ankyrin repeat domain 42 [Source:HGNC Symbol;Acc:HGNC:26752]) HSP 1 Score: 164.851 bits (416), Expect = 2.483e-44 Identity = 93/252 (36.90%), Postives = 142/252 (56.35%), Query Frame = 1
Query: 259 WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
WTA+H+A I G+DAC+++LIM + + D RGCT LHLAA HG + + L +D + +W P+ YAA+HGR+ ++LL++ G + + D +G P+HLAA EGHL K FL + L A ++NG L LA+ K I+++I+ E + + E + L +PGH+AA GDL ++ L+E G I +N+ + G T +HKA GHI+ ++WL+ GA I E D+
Sbjct: 66 WTASHIAAIRGQDACVQALIMNGANLTAQ-DDRGCTPLHLAATHGHSFTLQIM-----LRSGVDPSVTDKREWRPVHYAAFHGRLGCLQLLVKWGCSIEDVDYNGNLPVHLAAMEGHLHCFK-FLVSRMSSATQVLKAFNDNGENVLDLAQRFFKQNILQFIQGAEYEGKDLEDQETLAFPGHVAAFKGDLGMLKKLVEDGVININERADNGSTPMHKAAGQGHIECLQWLIKMGADSNITNKAGERPSDVA 310
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Human
Match: ANKRD42 (ankyrin repeat domain 42 [Source:HGNC Symbol;Acc:HGNC:26752]) HSP 1 Score: 90.1225 bits (222), Expect = 5.171e-20 Identity = 60/198 (30.30%), Postives = 103/198 (52.02%), Query Frame = 1
Query: 4 SMLNDEYLEESEKRRLLFAYITADDDARFKRAVKDIDPVEIRKIIMEDTDL----SLLHWAALKGSVVIVNFLIDKMEFPSDLDN-NAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRN-MQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTP 579
S + E ++++ F I DA VK + + +R + + D+ + LHWAA GS+ +++L + +D+ + WTA+H+A I G+DAC+++LIM + + D RGCT LHLAA HG + + L +D + +W P+ YAA+HGR+ ++LL++ G + + D +G P
Sbjct: 10 STSSRETANPCSRKKVHFGSI---HDAVRAGDVKQLSEIVVRGASINELDVLHKFTPLHWAAHSGSLECLHWL---LWHGADITHVTTRGWTASHIAAIRGQDACVQALIMNGANLTAQ-DDRGCTPLHLAATHGHSFTLQIM-----LRSGVDPSVTDKREWRPVHYAAFHGRLGCLQLLVKWGCSIEDVDYNGNLP 195 HSP 2 Score: 50.0618 bits (118), Expect = 2.856e-6 Identity = 46/144 (31.94%), Postives = 68/144 (47.22%), Query Frame = 1
Query: 469 QWTPLMYAAWHGRISIVELLIRHGVATDVTDVD--GRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQY-PGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRG 891
++TPL +AA G + + L+ HG D+T V G T H+AA G V+ + G + LTA D+ G T L LA T ++ + DP + D ++ P H AA +G L +Q LL K ++ D D G
Sbjct: 60 KFTPLHWAAHSGSLECLHWLLWHGA--DITHVTTRGWTASHIAAIRGQDACVQALIMNGAN-----LTAQDDRGCTPLHLAATHGHSFTLQIMLRSGVDP---SVTDKREWRPVHYAAFHGRLGCLQ-LLVKWGCSIEDVDYNG 192
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 77.0258 bits (188), Expect = 2.890e-14 Identity = 66/219 (30.14%), Postives = 105/219 (47.95%), Query Frame = 1
Query: 316 IMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
++R IN ++ G LHLA+ G + L + +DA R T L A+ G+ IV +L+ +G +V V+G TPL++AA E H +VKY L+ G ++ L+ D GFT L +A + D +V + + + + ++ P HIAA D LL + + T G+T LH A GH ++ + LL KGA++
Sbjct: 52 LLRAGTDINTSNANGLNSLHLASKEGHSEVVREL---IKRQAQVDAATRKGN-TALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGA---NQALSTED--GFTPLAVALQQGHDRVVAVLLENDSKGK-------VRLPALHIAAKKDD-TTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANV 253 HSP 2 Score: 73.559 bits (179), Expect = 3.996e-13 Identity = 91/323 (28.17%), Postives = 140/323 (43.34%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGK-DACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQ-WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCH-DIHKCLTAVDNNGFTALKLAETKSKDEIVKY-IRDKEK-DPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDT-DN-------------------------------RGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
L+ LH AA G +V+ L+ + S N H+A G DA L R P ++ T LH+AA HC H L + D R + +TPL A RI +VELL+++ A + T G TPLH+AA G + +V Y L++G + D+ G T L LA ++ ++V+ IR+ K D + E LQ P HIA+ G+ + LL+ G+ + T DN +G+T LH A G++++V+ LL +G + I+ Q T + +
Sbjct: 294 LTPLHCAARSGHDQVVDLLVVQGAPISAKTKNG--LAPLHMAAQGDHVDAARTLLYHRAP--VDDVTVDYLTPLHVAA-----HCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETV------RGETPLHLAARANQTDVVRVLIRNGAKVDAQARE----LQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVA 597 HSP 3 Score: 72.7886 bits (177), Expect = 6.286e-13 Identity = 82/297 (27.61%), Postives = 126/297 (42.42%), Query Frame = 1
Query: 151 DLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRG---CTLLHLAAAHGRYHCMHYLANFTRLL-----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVK--YIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+ S LH AA +G + L+D + L +T H+A G + L+ R V D G T LH+AA HY + +L + A +N +TPL AA ++ I L++ + G TPLHL+A EGH + +E G + A NNG TA+ L + + + Y E + +T + L H+A G L ++ L+E G+ V + YT LH+A GH + V++LL GAS +T +T + I
Sbjct: 524 NYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKG--FTPLHLASKYGNLEVVRLLLERGTPV----DIEGKNQVTPLHVAA--------HYNNDKVAMLLLENGASAKAAAKN-GYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSD-----VGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPL----HVACHFGQLNMVKFLVENGA-DVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 4 Score: 68.9366 bits (167), Expect = 8.890e-12 Identity = 100/397 (25.19%), Postives = 145/397 (36.52%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYL-----------------------ANFT---RLL----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVD---------------------------------GRTPLHLAAAEGHLPLVKYFLEEGCH-DIH---------------------------KCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDP--RTWELFDDLQYP---GH---------------IAAVNG----------DLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
L+ LH AA G V + L+D+ P+ N +T H+A + +E L+++ I G T LH+AA G + + YL AN T R+L +DAQ R +Q TPL A+ G IV LL++ G ++ T D G TPLHLA+ G+L +V+ LE G DI A NG+T L +A K++ EI + + DP ++ F L GH A NG D + + +L +N N GYT LH AC G +++VK+L+ GA + KT
Sbjct: 360 LTPLHVAAHCGHVRVAKLLLDRSADPNSRALNG--FTPLHIACKKNRIKVVE-LLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQ-TPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKT 752 HSP 5 Score: 60.8474 bits (146), Expect = 3.098e-9 Identity = 72/265 (27.17%), Postives = 108/265 (40.75%), Query Frame = 1
Query: 328 PEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLL----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLA---------------ETKSKDEIVKYIR------------------DKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
P+V ++ G T LH+AA +G N +LL N++ Q R+ +PL A GR ++ LL+ G D D TPLH AA GH +V + +G ++A NG L +A D V Y+ D+ DP + L + P HI A + + + LL K A+ T G T LH A G I+IV +LL +GA+ ++T+ ET + +
Sbjct: 220 PDVTSK---SGFTPLHIAAHYGH-------ENVGQLLLEKGANVNYQARH-NISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGA-----PISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRAL--NGFTPLHI-ACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLA 465 HSP 6 Score: 51.9878 bits (123), Expect = 1.677e-6 Identity = 49/195 (25.13%), Postives = 85/195 (43.59%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEI-WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIR 744
LH AA K + I + L ++F +D + + +T H++ G + L++ + + G T +HL A L N ++++ N +TPL A G++++V+ L+ +G TPLH AA +GH V+Y LE G + T G T L +A+ +V+ +R
Sbjct: 627 LHIAAKKNQMEIASTL---LQFKADPNAKSRAGFTPLHLSAQEGHKE-ISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGA---EINSKT-NAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTAT-----GQTPLSIAQRLGYVSVVETLR 808
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 77.0258 bits (188), Expect = 2.890e-14 Identity = 66/219 (30.14%), Postives = 105/219 (47.95%), Query Frame = 1
Query: 316 IMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
++R IN ++ G LHLA+ G + L + +DA R T L A+ G+ IV +L+ +G +V V+G TPL++AA E H +VKY L+ G ++ L+ D GFT L +A + D +V + + + + ++ P HIAA D LL + + T G+T LH A GH ++ + LL KGA++
Sbjct: 52 LLRAGTDINTSNANGLNSLHLASKEGHSEVVREL---IKRQAQVDAATRKGN-TALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGA---NQALSTED--GFTPLAVALQQGHDRVVAVLLENDSKGK-------VRLPALHIAAKKDD-TTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANV 253 HSP 2 Score: 73.559 bits (179), Expect = 3.996e-13 Identity = 91/323 (28.17%), Postives = 140/323 (43.34%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGK-DACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQ-WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCH-DIHKCLTAVDNNGFTALKLAETKSKDEIVKY-IRDKEK-DPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDT-DN-------------------------------RGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
L+ LH AA G +V+ L+ + S N H+A G DA L R P ++ T LH+AA HC H L + D R + +TPL A RI +VELL+++ A + T G TPLH+AA G + +V Y L++G + D+ G T L LA ++ ++V+ IR+ K D + E LQ P HIA+ G+ + LL+ G+ + T DN +G+T LH A G++++V+ LL +G + I+ Q T + +
Sbjct: 294 LTPLHCAARSGHDQVVDLLVVQGAPISAKTKNG--LAPLHMAAQGDHVDAARTLLYHRAP--VDDVTVDYLTPLHVAA-----HCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETV------RGETPLHLAARANQTDVVRVLIRNGAKVDAQARE----LQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVA 597 HSP 3 Score: 72.7886 bits (177), Expect = 6.286e-13 Identity = 82/297 (27.61%), Postives = 126/297 (42.42%), Query Frame = 1
Query: 151 DLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRG---CTLLHLAAAHGRYHCMHYLANFTRLL-----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVK--YIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+ S LH AA +G + L+D + L +T H+A G + L+ R V D G T LH+AA HY + +L + A +N +TPL AA ++ I L++ + G TPLHL+A EGH + +E G + A NNG TA+ L + + + Y E + +T + L H+A G L ++ L+E G+ V + YT LH+A GH + V++LL GAS +T +T + I
Sbjct: 524 NYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKG--FTPLHLASKYGNLEVVRLLLERGTPV----DIEGKNQVTPLHVAA--------HYNNDKVAMLLLENGASAKAAAKN-GYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSD-----VGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPL----HVACHFGQLNMVKFLVENGA-DVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 4 Score: 68.9366 bits (167), Expect = 8.890e-12 Identity = 100/397 (25.19%), Postives = 145/397 (36.52%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYL-----------------------ANFT---RLL----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVD---------------------------------GRTPLHLAAAEGHLPLVKYFLEEGCH-DIH---------------------------KCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDP--RTWELFDDLQYP---GH---------------IAAVNG----------DLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
L+ LH AA G V + L+D+ P+ N +T H+A + +E L+++ I G T LH+AA G + + YL AN T R+L +DAQ R +Q TPL A+ G IV LL++ G ++ T D G TPLHLA+ G+L +V+ LE G DI A NG+T L +A K++ EI + + DP ++ F L GH A NG D + + +L +N N GYT LH AC G +++VK+L+ GA + KT
Sbjct: 360 LTPLHVAAHCGHVRVAKLLLDRSADPNSRALNG--FTPLHIACKKNRIKVVE-LLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQ-TPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKT 752 HSP 5 Score: 60.8474 bits (146), Expect = 3.098e-9 Identity = 72/265 (27.17%), Postives = 108/265 (40.75%), Query Frame = 1
Query: 328 PEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLL----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLA---------------ETKSKDEIVKYIR------------------DKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
P+V ++ G T LH+AA +G N +LL N++ Q R+ +PL A GR ++ LL+ G D D TPLH AA GH +V + +G ++A NG L +A D V Y+ D+ DP + L + P HI A + + + LL K A+ T G T LH A G I+IV +LL +GA+ ++T+ ET + +
Sbjct: 220 PDVTSK---SGFTPLHIAAHYGH-------ENVGQLLLEKGANVNYQARH-NISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGA-----PISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRAL--NGFTPLHI-ACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLA 465 HSP 6 Score: 51.9878 bits (123), Expect = 1.677e-6 Identity = 49/195 (25.13%), Postives = 85/195 (43.59%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEI-WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIR 744
LH AA K + I + L ++F +D + + +T H++ G + L++ + + G T +HL A L N ++++ N +TPL A G++++V+ L+ +G TPLH AA +GH V+Y LE G + T G T L +A+ +V+ +R
Sbjct: 627 LHIAAKKNQMEIASTL---LQFKADPNAKSRAGFTPLHLSAQEGHKE-ISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGA---EINSKT-NAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTAT-----GQTPLSIAQRLGYVSVVETLR 808
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 77.0258 bits (188), Expect = 2.999e-14 Identity = 66/219 (30.14%), Postives = 105/219 (47.95%), Query Frame = 1
Query: 316 IMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
++R IN ++ G LHLA+ G + L + +DA R T L A+ G+ IV +L+ +G +V V+G TPL++AA E H +VKY L+ G ++ L+ D GFT L +A + D +V + + + + ++ P HIAA D LL + + T G+T LH A GH ++ + LL KGA++
Sbjct: 52 LLRAGTDINTSNANGLNSLHLASKEGHSEVVREL---IKRQAQVDAATRKGN-TALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGA---NQALSTED--GFTPLAVALQQGHDRVVAVLLENDSKGK-------VRLPALHIAAKKDD-TTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANV 253 HSP 2 Score: 73.1738 bits (178), Expect = 4.594e-13 Identity = 85/275 (30.91%), Postives = 125/275 (45.45%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGK-DACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQ-WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCH-DIHKCLTAVDNNGFTALKLAETKSKDEIVKY-IRDKEK-DPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
L+ LH AA G +V+ L+ + S N H+A G DA L R P ++ T LH+AA HC H L + D R + +TPL A RI +VELL+++ A + T G TPLH+AA G + +V Y L++G + D+ G T L LA ++ ++V+ IR+ K D + E LQ P HIA+ G+ + LL+ G+ + N T Y+ LH A G ++ LL A
Sbjct: 294 LTPLHCAARSGHDQVVDLLVVQGAPISAKTKNG--LAPLHMAAQGDHVDAARTLLYHRAP--VDDVTVDYLTPLHVAA-----HCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETV------RGETPLHLAARANQTDVVRVLIRNGAKVDAQARE----LQTPLHIASRLGNTDIVILLLQAGANS-NATTRDNYSPLHIAAKEGQEEVAGILLDHNA 548 HSP 3 Score: 72.7886 bits (177), Expect = 7.166e-13 Identity = 82/297 (27.61%), Postives = 126/297 (42.42%), Query Frame = 1
Query: 151 DLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRG---CTLLHLAAAHGRYHCMHYLANFTRLL-----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVK--YIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+ S LH AA +G + L+D + L +T H+A G + L+ R V D G T LH+AA HY + +L + A +N +TPL AA ++ I L++ + G TPLHL+A EGH + +E G + A NNG TA+ L + + + Y E + +T + L H+A G L ++ L+E G+ V + YT LH+A GH + V++LL GAS +T +T + I
Sbjct: 524 NYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKG--FTPLHLASKYGNLEVVRLLLERGTPV----DIEGKNQVTPLHVAA--------HYNNDKVAMLLLENGASAKAAAKN-GYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSD-----VGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPL----HVACHFGQLNMVKFLVENGA-DVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 4 Score: 68.9366 bits (167), Expect = 9.874e-12 Identity = 100/397 (25.19%), Postives = 145/397 (36.52%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYL-----------------------ANFT---RLL----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVD---------------------------------GRTPLHLAAAEGHLPLVKYFLEEGCH-DIH---------------------------KCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDP--RTWELFDDLQYP---GH---------------IAAVNG----------DLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
L+ LH AA G V + L+D+ P+ N +T H+A + +E L+++ I G T LH+AA G + + YL AN T R+L +DAQ R +Q TPL A+ G IV LL++ G ++ T D G TPLHLA+ G+L +V+ LE G DI A NG+T L +A K++ EI + + DP ++ F L GH A NG D + + +L +N N GYT LH AC G +++VK+L+ GA + KT
Sbjct: 360 LTPLHVAAHCGHVRVAKLLLDRSADPNSRALNG--FTPLHIACKKNRIKVVE-LLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQ-TPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKT 752 HSP 5 Score: 60.4622 bits (145), Expect = 3.874e-9 Identity = 72/265 (27.17%), Postives = 108/265 (40.75%), Query Frame = 1
Query: 328 PEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLL----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLA---------------ETKSKDEIVKYIR------------------DKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
P+V ++ G T LH+AA +G N +LL N++ Q R+ +PL A GR ++ LL+ G D D TPLH AA GH +V + +G ++A NG L +A D V Y+ D+ DP + L + P HI A + + + LL K A+ T G T LH A G I+IV +LL +GA+ ++T+ ET + +
Sbjct: 220 PDVTSK---SGFTPLHIAAHYGH-------ENVGQLLLEKGANVNYQARH-NISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGA-----PISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRAL--NGFTPLHI-ACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLA 465 HSP 6 Score: 51.9878 bits (123), Expect = 1.638e-6 Identity = 49/195 (25.13%), Postives = 85/195 (43.59%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEI-WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIR 744
LH AA K + I + L ++F +D + + +T H++ G + L++ + + G T +HL A L N ++++ N +TPL A G++++V+ L+ +G TPLH AA +GH V+Y LE G + T G T L +A+ +V+ +R
Sbjct: 627 LHIAAKKNQMEIASTL---LQFKADPNAKSRAGFTPLHLSAQEGHKE-ISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGA---EINSKT-NAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTAT-----GQTPLSIAQRLGYVSVVETLR 808
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 77.0258 bits (188), Expect = 2.999e-14 Identity = 66/219 (30.14%), Postives = 105/219 (47.95%), Query Frame = 1
Query: 316 IMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
++R IN ++ G LHLA+ G + L + +DA R T L A+ G+ IV +L+ +G +V V+G TPL++AA E H +VKY L+ G ++ L+ D GFT L +A + D +V + + + + ++ P HIAA D LL + + T G+T LH A GH ++ + LL KGA++
Sbjct: 52 LLRAGTDINTSNANGLNSLHLASKEGHSEVVREL---IKRQAQVDAATRKGN-TALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGA---NQALSTED--GFTPLAVALQQGHDRVVAVLLENDSKGK-------VRLPALHIAAKKDD-TTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANV 253 HSP 2 Score: 73.1738 bits (178), Expect = 4.594e-13 Identity = 85/275 (30.91%), Postives = 125/275 (45.45%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGK-DACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQ-WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCH-DIHKCLTAVDNNGFTALKLAETKSKDEIVKY-IRDKEK-DPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
L+ LH AA G +V+ L+ + S N H+A G DA L R P ++ T LH+AA HC H L + D R + +TPL A RI +VELL+++ A + T G TPLH+AA G + +V Y L++G + D+ G T L LA ++ ++V+ IR+ K D + E LQ P HIA+ G+ + LL+ G+ + N T Y+ LH A G ++ LL A
Sbjct: 294 LTPLHCAARSGHDQVVDLLVVQGAPISAKTKNG--LAPLHMAAQGDHVDAARTLLYHRAP--VDDVTVDYLTPLHVAA-----HCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETV------RGETPLHLAARANQTDVVRVLIRNGAKVDAQARE----LQTPLHIASRLGNTDIVILLLQAGANS-NATTRDNYSPLHIAAKEGQEEVAGILLDHNA 548 HSP 3 Score: 72.7886 bits (177), Expect = 7.166e-13 Identity = 82/297 (27.61%), Postives = 126/297 (42.42%), Query Frame = 1
Query: 151 DLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRG---CTLLHLAAAHGRYHCMHYLANFTRLL-----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVK--YIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+ S LH AA +G + L+D + L +T H+A G + L+ R V D G T LH+AA HY + +L + A +N +TPL AA ++ I L++ + G TPLHL+A EGH + +E G + A NNG TA+ L + + + Y E + +T + L H+A G L ++ L+E G+ V + YT LH+A GH + V++LL GAS +T +T + I
Sbjct: 524 NYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKG--FTPLHLASKYGNLEVVRLLLERGTPV----DIEGKNQVTPLHVAA--------HYNNDKVAMLLLENGASAKAAAKN-GYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSD-----VGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPL----HVACHFGQLNMVKFLVENGA-DVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 4 Score: 68.9366 bits (167), Expect = 9.874e-12 Identity = 100/397 (25.19%), Postives = 145/397 (36.52%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYL-----------------------ANFT---RLL----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVD---------------------------------GRTPLHLAAAEGHLPLVKYFLEEGCH-DIH---------------------------KCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDP--RTWELFDDLQYP---GH---------------IAAVNG----------DLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
L+ LH AA G V + L+D+ P+ N +T H+A + +E L+++ I G T LH+AA G + + YL AN T R+L +DAQ R +Q TPL A+ G IV LL++ G ++ T D G TPLHLA+ G+L +V+ LE G DI A NG+T L +A K++ EI + + DP ++ F L GH A NG D + + +L +N N GYT LH AC G +++VK+L+ GA + KT
Sbjct: 360 LTPLHVAAHCGHVRVAKLLLDRSADPNSRALNG--FTPLHIACKKNRIKVVE-LLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQ-TPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKT 752 HSP 5 Score: 60.4622 bits (145), Expect = 3.874e-9 Identity = 72/265 (27.17%), Postives = 108/265 (40.75%), Query Frame = 1
Query: 328 PEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLL----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLA---------------ETKSKDEIVKYIR------------------DKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
P+V ++ G T LH+AA +G N +LL N++ Q R+ +PL A GR ++ LL+ G D D TPLH AA GH +V + +G ++A NG L +A D V Y+ D+ DP + L + P HI A + + + LL K A+ T G T LH A G I+IV +LL +GA+ ++T+ ET + +
Sbjct: 220 PDVTSK---SGFTPLHIAAHYGH-------ENVGQLLLEKGANVNYQARH-NISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGA-----PISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRAL--NGFTPLHI-ACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLA 465 HSP 6 Score: 51.9878 bits (123), Expect = 1.638e-6 Identity = 49/195 (25.13%), Postives = 85/195 (43.59%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEI-WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIR 744
LH AA K + I + L ++F +D + + +T H++ G + L++ + + G T +HL A L N ++++ N +TPL A G++++V+ L+ +G TPLH AA +GH V+Y LE G + T G T L +A+ +V+ +R
Sbjct: 627 LHIAAKKNQMEIASTL---LQFKADPNAKSRAGFTPLHLSAQEGHKE-ISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGA---EINSKT-NAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTAT-----GQTPLSIAQRLGYVSVVETLR 808
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Celegans
Match: trp-4 (TRP (Transient receptor potential) channel family [Source:UniProtKB/TrEMBL;Acc:Q9GRV5]) HSP 1 Score: 77.0258 bits (188), Expect = 3.267e-14 Identity = 70/228 (30.70%), Postives = 109/228 (47.81%), Query Frame = 1
Query: 262 TAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVK 942
TA H+A G L L+++ +N G LHLAA HG ++ L L+A + Q T L +AA G++++ + L+ G + D G+TPLHLAA +VK FL+ ++ LTA+D+NGFT +A K +V+ + +K L+ H+AA G ++ LLE G+ A D ++ G T LH +G I I++
Sbjct: 922 TALHLAAFNGH-LSLVHLLLQHKAFVNSKSKTGEAPLHLAAQHGHVKVVNVLVQDHG--AALEAITLDNQ-TALHFAAKFGQLAVSQTLLALGANPNARDDKGQTPLHLAAENDFPDVVKLFLKMRNNN-RSVLTAIDHNGFTCAHIAAMKGSLAVVRELMMIDKPMVIQAKTKTLEATTLHMAAAGGHANIVKILLENGANA-EDENSHGMTALHLGAKNGFISILE 1143 HSP 2 Score: 72.4034 bits (176), Expect = 7.735e-13 Identity = 80/292 (27.40%), Postives = 126/292 (43.15%), Query Frame = 1
Query: 166 HWAALKGSVVIVN--FLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQC-RNMQWTPLMYAAWHGRISIVELLIRHGVAT--------------DVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYI---RDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
H AA+KGS+ +V +IDK E T H+A GG A + +++ +S G T LHL A +G + L F ++L +C R L AA++G V +++H AT + + G TPLHLAA GH LV+ L +G + T ++ L LA + +V + +++ + W + P H+AA NG + L+ +GS +N D G+T LH A +GH+ +VK + A +T
Sbjct: 1062 HIAAMKGSLAVVRELMMIDKPMVIQAKTKTLEA-TTLHMAAAGGH-ANIVKILLENGANAEDENSHGMTALHLGAKNG---FISILEAFDKILWK---RCSRKTGLNALHIAAFYGNSDFVNEMLKHVQATVRSEPPIYNHHVNKEFSTEYGFTPLHLAAQSGHDSLVRMLLNQGVQ-VDATSTTMN---VIPLHLAAQQGHIAVVGMLLSRSTQQQHAKDWRG----RTPLHLAAQNGHYEMVSLLIAQGS-NINVMDQNGWTGLHFATRAGHLSVVKLFIDSSADPLAET 1336 HSP 3 Score: 68.5514 bits (166), Expect = 1.296e-11 Identity = 70/229 (30.57%), Postives = 103/229 (44.98%), Query Frame = 1
Query: 289 GKDACLESLIMRKPEVINRPD-SRGCTLLHLAAAHGRYHCMHYLAN-----FTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKC-LTAVDNNGFTALKLAETKSKDEIVKY-IRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLL 951
G+DA L +L++ ++ P + T +H+AA G + + N +++ N Q +N W+PL+ A G + +L++H DV D GRT LHLAA GHL LV L+ HK + + G L LA ++V ++D L D Q H AA G L Q LL G+ N D++G T LH A + D+VK L
Sbjct: 824 GEDAKLVNLLIDYGGMVEMPSLNANETAMHMAARSGNQAVLLAMVNKIGAGAVQIVQN--KQSKN-GWSPLLEACARGHSGVANILLKHHARIDVFDEMGRTALHLAAFNGHLSLVHLLLQ------HKAFVNSKSKTGEAPLHLAAQHGHVKVVNVLVQDHGAALEAITL--DNQTALHFAAKFGQLAVSQTLLALGA-NPNARDDKGQTPLHLAAENDFPDVVKLFL 1040 HSP 4 Score: 62.7734 bits (151), Expect = 8.335e-10 Identity = 85/353 (24.08%), Postives = 136/353 (38.53%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSD---LDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAA-------------------------HGRYHCMHYLA-----NFTRLLPNLD------AQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYF---------LEEGCHDIHKCLTAVDNN----------------------------------GFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWI 975
LH AA G V +VN L+ + LDN TA H A G+ A ++L+ N D +G T LHLAA H + C H A R L +D A+ + ++ T L AA G +IV++L+ +G + + G T LHL A G + +++ F + G + +H + A N GFT L LA D +V+ + ++ ++ P H+AA G + + LL + + + D RG T LH A +GH ++V L+++G++I +
Sbjct: 957 LHLAAQHGHVKVVNVLVQDHGAALEAITLDNQ----TALHFAAKFGQLAVSQTLLALGANP-NARDDKGQTPLHLAAENDFPDVVKLFLKMRNNNRSVLTAIDHNGFTCAHIAAMKGSLAVVRELMMIDKPMVIQAKTKTLEATTLHMAAAGGHANIVKILLENGANAEDENSHGMTALHLGAKNGFISILEAFDKILWKRCSRKTGLNALH--IAAFYGNSDFVNEMLKHVQATVRSEPPIYNHHVNKEFSTEYGFTPLHLAAQSGHDSLVRMLLNQGVQVDATSTTMNV-IPLHLAAQQGHIAVVGMLLSRSTQQQHAKDWRGRTPLHLAAQNGHYEMVSLLIAQGSNINV 1301 HSP 5 Score: 62.3882 bits (150), Expect = 9.969e-10 Identity = 63/231 (27.27%), Postives = 100/231 (43.29%), Query Frame = 1
Query: 148 TDLSLLHWAALKGSVVIVNFLIDKMEF-----PSDLDN--NAEI-----WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQW---TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQY 795
T L+ LH AA G+ VN ++ ++ P ++ N E +T H+A G D+ + L+ + +V + LHLAA G ++A LL Q W TPL AA +G +V LLI G +V D +G T LH A GHL +VK F++ + A G L A + E ++++ ++ D T +L +D ++
Sbjct: 1157 TGLNALHIAAFYGNSDFVNEMLKHVQATVRSEPPIYNHHVNKEFSTEYGFTPLHLAAQSGHDSLVRMLLNQGVQVDATSTTMNVIPLHLAAQQG------HIAVVGMLLSRSTQQQHAKDWRGRTPLHLAAQNGHYEMVSLLIAQGSNINVMDQNGWTGLHFATRAGHLSVVKLFIDSSADPL-----AETKEGKVPLCFAAAHNHIECLRFLLKQKHD--THQLMEDRKF 1374 HSP 6 Score: 55.4546 bits (132), Expect = 1.314e-7 Identity = 52/172 (30.23%), Postives = 80/172 (46.51%), Query Frame = 1
Query: 478 PLMYAAWHGRISIVELLIRHGVATDVT---DVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNN-GFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEK--GSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWI 975
P+ A G ++IVELL+ G + + T D +G T LHLAA G++ V+ + GC + + V N G T L ++K + D + D + P H+AA GD ++ L++K GSI D G T+LH A SGH L +G +++
Sbjct: 460 PIHLAFKFGNVNIVELLLS-GPSDEQTRKADGNGDTLLHLAARSGNIEAVRTAIAAGCDNAN-----VQNRVGRTPLHEVAEVGDQNMLKIMFKLRADANIHDKED--KTPVHVAAERGDTSMVESLIDKFGGSIRARTRD--GSTLLHIAACSGHTSTALAFLKRGVPLFM 621 HSP 7 Score: 51.9878 bits (123), Expect = 1.689e-6 Identity = 45/131 (34.35%), Postives = 57/131 (43.51%), Query Frame = 1
Query: 262 TAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIH 654
T HVA G + +ESLI + I G TLLH+AA G H LA R +P + L AA G +V++LI G DV D T LH+A G +V+ L G DIH
Sbjct: 561 TPVHVAAERGDTSMVESLIDKFGGSIRARTRDGSTLLHIAACSG--HTSTALAFLKRGVPLFMPNKKGALG--LHSAAAAGFNDVVKMLIARGTNVDVRTRDNYTALHVAVQSGKASVVETLLGSGA-DIH 686 HSP 8 Score: 50.0618 bits (118), Expect = 6.476e-6 Identity = 75/297 (25.25%), Postives = 126/297 (42.42%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLD-NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAA----AHGRYHCMHYLANFTRL-LPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEE-----GCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDD----LQYPG--------HIAAVNGDLLNIQGLLEK-GSIAVNDTDNR----GYTMLHKACISGHIDIVKWLLSKGASI 969
LH AA G +V LI + +++D + +TA HVAV GK + +E+L+ ++ + G T LH+AA A R M L + + + +D + T L AA G I+ LL+ + ++ G TPL +AA + L+ + + + N+GFTAL A + + + +D + L D ++ P H+AA +G+ + ++ K G+ AV N+ G++ L +AC GH + LL A I
Sbjct: 630 LHSAAAAGFNDVVKMLIAR---GTNVDVRTRDNYTALHVAVQSGKASVVETLLGSGADIHVKGGELGQTALHIAASLNGAESRDCAMMLLKSGGQPDVAQMDGE------TCLHIAARSGNKDIMRLLLDENADSKISSKIGETPLQVAAKSCNFEAASMILKHLSEVLTQEQLKEHVNHRTNDGFTALHYAAEIEQ----RQLHFPGEDAKLVNLLIDYGGMVEMPSLNANETAMHMAARSGNQAVLLAMVNKIGAGAVQIVQNKQSKNGWSPLLEACARGHSGVANILLKHHARI 913
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Fly
Match: CG10011 (gene:FBgn0039590 transcript:FBtr0085319) HSP 1 Score: 89.7373 bits (221), Expect = 4.409e-18 Identity = 87/293 (29.69%), Postives = 133/293 (45.39%), Query Frame = 1
Query: 139 MEDTDLSLLHWAALKGSVVIVNFLIDKMEFP--SDLDNNAEIW---TAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCR-NMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVD---NNGFTALKLAETKSKDEIVKY-------IRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
+++ +S L ++ +G+ + L++ P +D +W TA H V+ L++ I+ DS G T+L + AA G + L L LD R N WTPL YAA+ G + L+ G D D +G+T LHLAA EG L V+ L DIH VD ++G TA +LA + + V++ + K+ D RT +I A+ L ++ LL+ ++ VN D+ G T LH A GH D+VK L+ GA +
Sbjct: 1448 LDNDGMSPLLVSSFEGNAEVCELLLENAADPDLADFMGRTPLWAACTAGHATVV--------KLLLFWGCGIDCMDSEGRTVLSIGAAQGNVETVRQL-----LDRGLDETHRDNAGWTPLHYAAFEGFHEVCLQLLESGAKIDECDNEGKTALHLAAQEGRLHCVQALL-----DIHSSF--VDQKAHDGKTAFRLACLEGHMDTVEFLLKFCCDVNSKDADSRTTL---------YILALENKLEIVKYLLDMTNVDVNIPDSEGRTALHVAAWQGHADMVKTLIEAGADV 1711 HSP 2 Score: 63.1586 bits (152), Expect = 8.091e-10 Identity = 56/202 (27.72%), Postives = 95/202 (47.03%), Query Frame = 1
Query: 172 AALKGSVVIVNFLIDKMEFPSDLDN-NAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVK----YIRDKEKDP 762
A L+G + V FL ++F D+++ +A+ T ++ + K ++ L+ +N PDS G T LH+AA G + L + ++D + R TPL AW G ++ +L+ +G D G T L ++A EGH V L+ G + D+ G T +KLA S+ I+K Y +++ +P
Sbjct: 1625 ACLEGHMDTVEFL---LKFCCDVNSKDADSRTTLYILALENKLEIVKYLLDMTNVDVNIPDSEGRTALHVAAWQGHADMVKTLIEAGADVNSMDLEAR----TPLHSCAWQGNHDVMNILLYYGALADHACKQGATALGISAQEGHEKCVIALLQFGANPYKS-----DHCGRTPIKLAAKSSRTSILKIFESYTKNEASNP 1814 HSP 3 Score: 59.3066 bits (142), Expect = 1.079e-8 Identity = 55/181 (30.39%), Postives = 83/181 (45.86%), Query Frame = 1
Query: 475 TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTAL---KLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDI 1008
T L AAW G I++LLI G + D GRT L A+ GH +V+ LE G + H +D +G +AL L + +++ + D + T +L +D P +++ G+ + LLE + + D G T L AC +GH +VK LL G I TV+ I
Sbjct: 1352 TALRAAAWSGHEDILKLLIESGADVNSVDRQGRTSLIAASYMGHYDIVEILLENGANVNH-----LDLDGRSALCVAALCGSSGYSKVISTLLDHGAN--TDQLDNDGMSPLLVSSFEGNAEVCELLLENAADP-DLADFMGRTPLWAACTAGHATVVKLLLFWGCGIDCMDSEGRTVLSI 1524 HSP 4 Score: 55.8398 bits (133), Expect = 1.472e-7 Identity = 53/220 (24.09%), Postives = 87/220 (39.55%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQW------------------------------TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIV 732
LH+AA +G + L++ + DN E TA H+A G+ C+++L+ +++ G T LA G + +L F + + DA R + T L AAW G +V+ LI G + D++ RTPLH A +G+ ++ L G H C G TAL ++ + ++ V
Sbjct: 1555 LHYAAFEGFHEVCLQLLESGAKIDECDN--EGKTALHLAAQEGRLHCVQALLDIHSSFVDQKAHDGKTAFRLACLEGHMDTVEFLLKFCCDVNSKDADSRTTLYILALENKLEIVKYLLDMTNVDVNIPDSEGRTALHVAAWQGHADMVKTLIEAGADVNSMDLEARTPLHSCAWQGNHDVMNILLYYGALADHAC-----KQGATALGISAQEGHEKCV 1767
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0344222) HSP 1 Score: 88.5817 bits (218), Expect = 9.808e-18 Identity = 89/282 (31.56%), Postives = 128/282 (45.39%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGK-DACLESLIMRKP--EVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQ-WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYI--RDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
L+ LH AA G +V+ L+++ S N H+A G DA L R P EV T+ +L A H HC H L N DA R + +TPL A R+ +VELL+RHG + T G TPLH+AA G + +V Y L+ HD + V G T L LA ++ +I++ + + D R E Q P HIA+ G++ + LL+ G+ V+ T YT LH A G ++ L+ GA++ T
Sbjct: 272 LTPLHCAARSGHEQVVDMLLERGAPISAKTKNG--LAPLHMAAQGEHVDAARILLYHRAPVDEV---------TVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQ---HDASPDVPTV--RGETPLHLAARANQTDIIRILLRNGAQVDARARE----QQTPLHIASRLGNVDIVMLLLQHGA-QVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAAT 532 HSP 2 Score: 84.3445 bits (207), Expect = 2.209e-16 Identity = 75/274 (27.37%), Postives = 123/274 (44.89%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLD-NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
LH A+ G+V IV L ++ + +D +++TA H+A G+D + ++++ ++ +G T LHL A +G L ++DAQ +N TPL A + + LL+ G + T +G TPLH+AA + + + LE G A GFT L L+ + EI + E + P H+ A D +N+ +LEK ++ GYT LH A G ++V++LL GA++ T
Sbjct: 473 LHIASRLGNVDIVMLL---LQHGAQVDATTKDMYTALHIAAKEGQDE-VAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEA---DVDAQGKN-GVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGA-----LANAESKAGFTPLHLSSQEGHAEISNLLI--EHKAAVNHPAKNGLTPMHLCA-QEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAAT 730 HSP 3 Score: 75.485 bits (184), Expect = 1.171e-13 Identity = 76/271 (28.04%), Postives = 115/271 (42.44%), Query Frame = 1
Query: 244 NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCH---------------DIHKCLTAVDNN---------GFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKG-SIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
+NA A H+A G + L +R+ +++ +G T LH+A+ G+ + L +++ Q +N +TPL AA ++V LL+ +G + DG TPL +A +GH +V LE D+ +DN+ GFT L +A I + K D + ++ P H+AA G + LLEKG +I D G T LH A SGH +V LL +GA I KT
Sbjct: 40 SNANGLNALHLASKDGHIHVVSEL-LRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNA---SVNVQSQN-GFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGAD-VNYSAKHNIS-PLHVAAKWGKTNMVSLLLEKGGNIEAKTRD--GLTPLHCAARSGHEQVVDMLLERGAPISAKT 301 HSP 4 Score: 74.3294 bits (181), Expect = 2.547e-13 Identity = 74/275 (26.91%), Postives = 119/275 (43.27%), Query Frame = 1
Query: 145 DTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
++ L+ LH AA G + IV +L+ P E T H+A + + L+ +V R + T LH+A+ G + L +DA ++M +T L AA G+ + +LI +G A D G TPLHL A GH+ + + L++ + A NG T L +A + ++ + +K P + P HIAA + LLE G++A N G+T LH + GH +I L+ A++
Sbjct: 401 ESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGE--TPLHLAARANQTDIIRILLRNGAQVDARAREQQ-TPLHIASRLGNVDIVMLLLQHGA---QVDATTKDM-YTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEAD-----VDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHAT--AKNGHTPLHIAARKNQMDIATTLLEYGALA-NAESKAGFTPLHLSSQEGHAEISNLLIEHKAAV 660 HSP 5 Score: 73.559 bits (179), Expect = 5.310e-13 Identity = 85/294 (28.91%), Postives = 130/294 (44.22%), Query Frame = 1
Query: 136 IMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAE-IWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYI-RDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+ + + LH A+ G+ I N LI K +D++ +A+ + HVA GK + SL++ K I G T LH AA G + L R P + A+ +N PL AA + +L+ H D VD T LH+AA GH+ + K L+ + L NGFT L +A K++ ++V+ + R T E P H+AA G +NI L + + + RG T LH A + DI++ LL GA + + Q+T + I
Sbjct: 200 VTSKSGFTPLHIASHYGNQNIANLLIQKG---ADVNYSAKHNISPLHVAAKWGKTNMV-SLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDML--LERGAP-ISAKTKN-GLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARAL-----NGFTPLHIACKKNRLKVVELLLRHGASISATTE---SGLTPLHVAAFMG-CMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIA 476 HSP 6 Score: 72.7886 bits (177), Expect = 7.473e-13 Identity = 63/217 (29.03%), Postives = 101/217 (46.54%), Query Frame = 1
Query: 337 INRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVND-----TDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
IN ++ G LHLA+ G +H ++ R +D+ + T L A+ G+ +V+LL+ H + +V +G TPL++AA E H +V+ L G + + +GFT L +A + D++V + E D R ++ P HIAA D+ LL+ ND T G+T LH A G+ +I L+ KGA +
Sbjct: 37 INTSNANGLNALHLASKDGH---IHVVSELLRRGAIVDSATKKGN-TALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQ-----SLATEDGFTPLAVAMQQGHDKVVAVLL--ESDTR-----GKVRLPALHIAAKKDDVKAATLLLD------NDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADV 231 HSP 7 Score: 56.6102 bits (135), Expect = 8.636e-8 Identity = 53/185 (28.65%), Postives = 79/185 (42.70%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEI---WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKC-LTAVDNNGFTALKLA 705
LH AA K + I L++ NAE +T H++ G A + +L++ +N P G T +HL A + L N+D + +TPL A+ G+ ++V L+++G D G TPLH A +GH +V LE HK A NG T L +A
Sbjct: 605 LHIAARKNQMDIATTLLEYGAL-----ANAESKAGFTPLHLSSQEGH-AEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGA---NIDMATK-AGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLE------HKANANAQTVNGQTPLHIA 773
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303122) HSP 1 Score: 88.5817 bits (218), Expect = 1.104e-17 Identity = 89/282 (31.56%), Postives = 128/282 (45.39%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGK-DACLESLIMRKP--EVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQ-WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYI--RDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
L+ LH AA G +V+ L+++ S N H+A G DA L R P EV T+ +L A H HC H L N DA R + +TPL A R+ +VELL+RHG + T G TPLH+AA G + +V Y L+ HD + V G T L LA ++ +I++ + + D R E Q P HIA+ G++ + LL+ G+ V+ T YT LH A G ++ L+ GA++ T
Sbjct: 272 LTPLHCAARSGHEQVVDMLLERGAPISAKTKNG--LAPLHMAAQGEHVDAARILLYHRAPVDEV---------TVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQ---HDASPDVPTV--RGETPLHLAARANQTDIIRILLRNGAQVDARARE----QQTPLHIASRLGNVDIVMLLLQHGA-QVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAAT 532 HSP 2 Score: 84.3445 bits (207), Expect = 2.464e-16 Identity = 75/274 (27.37%), Postives = 123/274 (44.89%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLD-NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
LH A+ G+V IV L ++ + +D +++TA H+A G+D + ++++ ++ +G T LHL A +G L ++DAQ +N TPL A + + LL+ G + T +G TPLH+AA + + + LE G A GFT L L+ + EI + E + P H+ A D +N+ +LEK ++ GYT LH A G ++V++LL GA++ T
Sbjct: 473 LHIASRLGNVDIVMLL---LQHGAQVDATTKDMYTALHIAAKEGQDE-VAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEA---DVDAQGKN-GVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGA-----LANAESKAGFTPLHLSSQEGHAEISNLL--IEHKAAVNHPAKNGLTPMHLCA-QEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAAT 730 HSP 3 Score: 75.485 bits (184), Expect = 1.209e-13 Identity = 76/271 (28.04%), Postives = 115/271 (42.44%), Query Frame = 1
Query: 244 NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCH---------------DIHKCLTAVDNN---------GFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKG-SIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
+NA A H+A G + L +R+ +++ +G T LH+A+ G+ + L +++ Q +N +TPL AA ++V LL+ +G + DG TPL +A +GH +V LE D+ +DN+ GFT L +A I + K D + ++ P H+AA G + LLEKG +I D G T LH A SGH +V LL +GA I KT
Sbjct: 40 SNANGLNALHLASKDGHIHVVSEL-LRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNA---SVNVQSQN-GFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGAD-VNYSAKHNIS-PLHVAAKWGKTNMVSLLLEKGGNIEAKTRD--GLTPLHCAARSGHEQVVDMLLERGAPISAKT 301 HSP 4 Score: 74.3294 bits (181), Expect = 2.991e-13 Identity = 74/275 (26.91%), Postives = 119/275 (43.27%), Query Frame = 1
Query: 145 DTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
++ L+ LH AA G + IV +L+ P E T H+A + + L+ +V R + T LH+A+ G + L +DA ++M +T L AA G+ + +LI +G A D G TPLHL A GH+ + + L++ + A NG T L +A + ++ + +K P + P HIAA + LLE G++A N G+T LH + GH +I L+ A++
Sbjct: 401 ESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGE--TPLHLAARANQTDIIRILLRNGAQVDARAREQQ-TPLHIASRLGNVDIVMLLLQHGA---QVDATTKDM-YTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEAD-----VDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHAT--AKNGHTPLHIAARKNQMDIATTLLEYGALA-NAESKAGFTPLHLSSQEGHAEISNLLIEHKAAV 660 HSP 5 Score: 73.1738 bits (178), Expect = 5.924e-13 Identity = 85/294 (28.91%), Postives = 130/294 (44.22%), Query Frame = 1
Query: 136 IMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAE-IWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYI-RDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+ + + LH A+ G+ I N LI K +D++ +A+ + HVA GK + SL++ K I G T LH AA G + L R P + A+ +N PL AA + +L+ H D VD T LH+AA GH+ + K L+ + L NGFT L +A K++ ++V+ + R T E P H+AA G +NI L + + + RG T LH A + DI++ LL GA + + Q+T + I
Sbjct: 200 VTSKSGFTPLHIASHYGNQNIANLLIQKG---ADVNYSAKHNISPLHVAAKWGKTNMV-SLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDML--LERGAP-ISAKTKN-GLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARAL-----NGFTPLHIACKKNRLKVVELLLRHGASISATTE---SGLTPLHVAAFMG-CMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIA 476 HSP 6 Score: 72.7886 bits (177), Expect = 7.719e-13 Identity = 63/217 (29.03%), Postives = 101/217 (46.54%), Query Frame = 1
Query: 337 INRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVND-----TDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
IN ++ G LHLA+ G +H ++ R +D+ + T L A+ G+ +V+LL+ H + +V +G TPL++AA E H +V+ L G + + +GFT L +A + D++V + E D R ++ P HIAA D+ LL+ ND T G+T LH A G+ +I L+ KGA +
Sbjct: 37 INTSNANGLNALHLASKDGH---IHVVSELLRRGAIVDSATKKGN-TALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQ-----SLATEDGFTPLAVAMQQGHDKVVAVLL--ESDTR-----GKVRLPALHIAAKKDDVKAATLLLD------NDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADV 231 HSP 7 Score: 56.225 bits (134), Expect = 9.471e-8 Identity = 53/185 (28.65%), Postives = 79/185 (42.70%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEI---WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKC-LTAVDNNGFTALKLA 705
LH AA K + I L++ NAE +T H++ G A + +L++ +N P G T +HL A + L N+D + +TPL A+ G+ ++V L+++G D G TPLH A +GH +V LE HK A NG T L +A
Sbjct: 605 LHIAARKNQMDIATTLLEYGAL-----ANAESKAGFTPLHLSSQEGH-AEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGA---NIDMATK-AGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLE------HKANANAQTVNGQTPLHIA 773
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303123) HSP 1 Score: 88.5817 bits (218), Expect = 1.291e-17 Identity = 89/282 (31.56%), Postives = 128/282 (45.39%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGK-DACLESLIMRKP--EVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQ-WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYI--RDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
L+ LH AA G +V+ L+++ S N H+A G DA L R P EV T+ +L A H HC H L N DA R + +TPL A R+ +VELL+RHG + T G TPLH+AA G + +V Y L+ HD + V G T L LA ++ +I++ + + D R E Q P HIA+ G++ + LL+ G+ V+ T YT LH A G ++ L+ GA++ T
Sbjct: 272 LTPLHCAARSGHEQVVDMLLERGAPISAKTKNG--LAPLHMAAQGEHVDAARILLYHRAPVDEV---------TVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQ---HDASPDVPTV--RGETPLHLAARANQTDIIRILLRNGAQVDARARE----QQTPLHIASRLGNVDIVMLLLQHGA-QVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAAT 532 HSP 2 Score: 83.9593 bits (206), Expect = 3.024e-16 Identity = 76/277 (27.44%), Postives = 126/277 (45.49%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLD-NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKE---KDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
LH A+ G+V IV L ++ + +D +++TA H+A G+D + ++++ ++ +G T LHL A +G L + ++DAQ +N TPL A + + LL+ G + T +G TPLH+AA + + + LE G A GFT L L+ + EI + + + P L P H+ A D +N+ +LEK ++ GYT LH A G ++V++LL GA++ T
Sbjct: 473 LHIASRLGNVDIVMLL---LQHGAQVDATTKDMYTALHIAAKEGQDE-VAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLL---LQKEADVDAQGKN-GVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGA-----LANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGL-----TPMHLCA-QEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAAT 730 HSP 3 Score: 75.485 bits (184), Expect = 1.329e-13 Identity = 76/271 (28.04%), Postives = 116/271 (42.80%), Query Frame = 1
Query: 244 NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCH---------------DIHKCLTAVDNN---------GFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKG-SIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
+NA A H+A G + S ++R+ +++ +G T LH+A+ G+ + L +++ Q +N +TPL AA ++V LL+ +G + DG TPL +A +GH +V LE D+ +DN+ GFT L +A I + K D + ++ P H+AA G + LLEKG +I D G T LH A SGH +V LL +GA I KT
Sbjct: 40 SNANGLNALHLASKDGHIHVV-SELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNA---SVNVQSQN-GFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGAD-VNYSAKHNIS-PLHVAAKWGKTNMVSLLLEKGGNIEAKTRD--GLTPLHCAARSGHEQVVDMLLERGAPISAKT 301 HSP 4 Score: 73.1738 bits (178), Expect = 6.124e-13 Identity = 65/217 (29.95%), Postives = 102/217 (47.00%), Query Frame = 1
Query: 337 INRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVND-----TDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
IN ++ G LHLA+ G +H ++ R +D+ + T L A+ G+ +V+LL+ H + +V +G TPL++AA E H +V+ L G ++ L D GFT L +A + D++V + E D R ++ P HIAA D+ LL+ ND T G+T LH A G+ +I L+ KGA +
Sbjct: 37 INTSNANGLNALHLASKDGH---IHVVSELLRRGAIVDSATKKGN-TALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGA---NQSLATED--GFTPLAVAMQQGHDKVVAVLL--ESDTR-----GKVRLPALHIAAKKDDVKAATLLLD------NDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADV 231 HSP 5 Score: 72.7886 bits (177), Expect = 7.776e-13 Identity = 74/275 (26.91%), Postives = 119/275 (43.27%), Query Frame = 1
Query: 145 DTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
++ L+ LH AA G + IV +L+ P E T H+A + + L+ +V R + T LH+A+ G + L +DA ++M +T L AA G+ + +LI +G A D G TPLHL A GH+ + + L++ + A NG T L +A + ++ + +K P + P HIAA + LLE G++A N G+T LH + GH +I L+ A++
Sbjct: 401 ESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGE--TPLHLAARANQTDIIRILLRNGAQVDARAREQ-QTPLHIASRLGNVDIVMLLLQHGA---QVDATTKDM-YTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEAD-----VDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHAT--AKNGHTPLHIAARKNQMDIATTLLEYGALA-NAESKAGFTPLHLSSQEGHAEISNLLIEHKAAV 660 HSP 6 Score: 72.4034 bits (176), Expect = 1.066e-12 Identity = 85/294 (28.91%), Postives = 130/294 (44.22%), Query Frame = 1
Query: 136 IMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAE-IWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYI-RDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+ + + LH A+ G+ I N LI K +D++ +A+ + HVA GK + SL++ K I G T LH AA G + L R P + A+ +N PL AA + +L+ H D VD T LH+AA GH+ + K L+ + L NGFT L +A K++ ++V+ + R T E P H+AA G +NI L + + + RG T LH A + DI++ LL GA + + Q+T + I
Sbjct: 200 VTSKSGFTPLHIASHYGNQNIANLLIQKG---ADVNYSAKHNISPLHVAAKWGKTNMV-SLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDML--LERGAP-ISAKTKN-GLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARAL-----NGFTPLHIACKKNRLKVVELLLRHGASISATTE---SGLTPLHVAAFMG-CMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIA 476 HSP 7 Score: 57.7658 bits (138), Expect = 4.128e-8 Identity = 53/185 (28.65%), Postives = 80/185 (43.24%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEI---WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKC-LTAVDNNGFTALKLA 705
LH AA K + I L++ NAE +T H++ G A + +L++ +N P G T +HL A + L + N+D + +TPL A+ G+ ++V L+++G D G TPLH A +GH +V LE HK A NG T L +A
Sbjct: 605 LHIAARKNQMDIATTLLEYGAL-----ANAESKAGFTPLHLSSQEGH-AEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILE---KNGANIDMATK-AGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLE------HKANANAQTVNGQTPLHIA 773
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303113) HSP 1 Score: 88.1965 bits (217), Expect = 1.399e-17 Identity = 89/282 (31.56%), Postives = 128/282 (45.39%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGK-DACLESLIMRKP--EVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQ-WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYI--RDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
L+ LH AA G +V+ L+++ S N H+A G DA L R P EV T+ +L A H HC H L N DA R + +TPL A R+ +VELL+RHG + T G TPLH+AA G + +V Y L+ HD + V G T L LA ++ +I++ + + D R E Q P HIA+ G++ + LL+ G+ V+ T YT LH A G ++ L+ GA++ T
Sbjct: 272 LTPLHCAARSGHEQVVDMLLERGAPISAKTKNG--LAPLHMAAQGEHVDAARILLYHRAPVDEV---------TVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQ---HDASPDVPTV--RGETPLHLAARANQTDIIRILLRNGAQVDARARE----QQTPLHIASRLGNVDIVMLLLQHGA-QVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAAT 532 HSP 2 Score: 83.9593 bits (206), Expect = 3.192e-16 Identity = 76/277 (27.44%), Postives = 126/277 (45.49%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLD-NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKE---KDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
LH A+ G+V IV L ++ + +D +++TA H+A G+D + ++++ ++ +G T LHL A +G L + ++DAQ +N TPL A + + LL+ G + T +G TPLH+AA + + + LE G A GFT L L+ + EI + + + P L P H+ A D +N+ +LEK ++ GYT LH A G ++V++LL GA++ T
Sbjct: 473 LHIASRLGNVDIVMLL---LQHGAQVDATTKDMYTALHIAAKEGQDE-VAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLL---LQKEADVDAQGKN-GVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGA-----LANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGL-----TPMHLCA-QEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAAT 730 HSP 3 Score: 75.485 bits (184), Expect = 1.344e-13 Identity = 76/271 (28.04%), Postives = 116/271 (42.80%), Query Frame = 1
Query: 244 NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCH---------------DIHKCLTAVDNN---------GFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKG-SIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
+NA A H+A G + S ++R+ +++ +G T LH+A+ G+ + L +++ Q +N +TPL AA ++V LL+ +G + DG TPL +A +GH +V LE D+ +DN+ GFT L +A I + K D + ++ P H+AA G + LLEKG +I D G T LH A SGH +V LL +GA I KT
Sbjct: 40 SNANGLNALHLASKDGHIHVV-SELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNA---SVNVQSQN-GFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGAD-VNYSAKHNIS-PLHVAAKWGKTNMVSLLLEKGGNIEAKTRD--GLTPLHCAARSGHEQVVDMLLERGAPISAKT 301 HSP 4 Score: 73.1738 bits (178), Expect = 6.352e-13 Identity = 65/217 (29.95%), Postives = 102/217 (47.00%), Query Frame = 1
Query: 337 INRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVND-----TDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
IN ++ G LHLA+ G +H ++ R +D+ + T L A+ G+ +V+LL+ H + +V +G TPL++AA E H +V+ L G ++ L D GFT L +A + D++V + E D R ++ P HIAA D+ LL+ ND T G+T LH A G+ +I L+ KGA +
Sbjct: 37 INTSNANGLNALHLASKDGH---IHVVSELLRRGAIVDSATKKGN-TALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGA---NQSLATED--GFTPLAVAMQQGHDKVVAVLL--ESDTR-----GKVRLPALHIAAKKDDVKAATLLLD------NDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADV 231 HSP 5 Score: 72.7886 bits (177), Expect = 8.417e-13 Identity = 74/275 (26.91%), Postives = 119/275 (43.27%), Query Frame = 1
Query: 145 DTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
++ L+ LH AA G + IV +L+ P E T H+A + + L+ +V R + T LH+A+ G + L +DA ++M +T L AA G+ + +LI +G A D G TPLHL A GH+ + + L++ + A NG T L +A + ++ + +K P + P HIAA + LLE G++A N G+T LH + GH +I L+ A++
Sbjct: 401 ESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGE--TPLHLAARANQTDIIRILLRNGAQVDARAREQ-QTPLHIASRLGNVDIVMLLLQHGA---QVDATTKDM-YTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEAD-----VDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHAT--AKNGHTPLHIAARKNQMDIATTLLEYGALA-NAESKAGFTPLHLSSQEGHAEISNLLIEHKAAV 660 HSP 6 Score: 72.4034 bits (176), Expect = 1.144e-12 Identity = 85/294 (28.91%), Postives = 130/294 (44.22%), Query Frame = 1
Query: 136 IMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAE-IWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYI-RDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+ + + LH A+ G+ I N LI K +D++ +A+ + HVA GK + SL++ K I G T LH AA G + L R P + A+ +N PL AA + +L+ H D VD T LH+AA GH+ + K L+ + L NGFT L +A K++ ++V+ + R T E P H+AA G +NI L + + + RG T LH A + DI++ LL GA + + Q+T + I
Sbjct: 200 VTSKSGFTPLHIASHYGNQNIANLLIQKG---ADVNYSAKHNISPLHVAAKWGKTNMV-SLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDML--LERGAP-ISAKTKN-GLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARAL-----NGFTPLHIACKKNRLKVVELLLRHGASISATTE---SGLTPLHVAAFMG-CMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIA 476 HSP 7 Score: 57.7658 bits (138), Expect = 3.998e-8 Identity = 53/185 (28.65%), Postives = 80/185 (43.24%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEI---WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKC-LTAVDNNGFTALKLA 705
LH AA K + I L++ NAE +T H++ G A + +L++ +N P G T +HL A + L + N+D + +TPL A+ G+ ++V L+++G D G TPLH A +GH +V LE HK A NG T L +A
Sbjct: 605 LHIAARKNQMDIATTLLEYGAL-----ANAESKAGFTPLHLSSQEGH-AEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILE---KNGANIDMATK-AGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLE------HKANANAQTVNGQTPLHIA 773
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Zebrafish
Match: ripk4 (receptor-interacting serine-threonine kinase 4 [Source:ZFIN;Acc:ZDB-GENE-040426-2042]) HSP 1 Score: 98.9821 bits (245), Expect = 6.041e-21 Identity = 84/268 (31.34%), Postives = 117/268 (43.66%), Query Frame = 1
Query: 259 WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRH-GVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVD-------------------------------NNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
+T H A G +A L L++ + IN D++G T H+A HG+ + + L L D + WT L AAW G + IV+LL++ G D DGR+PLHLA+ G + + +E G ++H LT+ D NG TAL LA K VK + + DP + + DL+ P H+AA NG ++ LL S N D G T LH A GH D + LL GA
Sbjct: 502 YTPLHFAAQNGDEA-LTRLLLDRSASINETDAQGRTPTHIACHHGQENVVRVL-----LSRGADVHVKGKDDWTALHLAAWKGHLGIVKLLVKQAGADVDGQTSDGRSPLHLASQRGQYRVARILVELGA-NVH--LTSDDLYAPLHVAAETGHTSTSRLLVKHDADIKSRTANGCTALHLASQKGHLPTVKMLLAEGADPES--VNHDLRTPCHLAAQNGHCEVLKELLRSCSDVANAQDRNGLTALHLAVSGGHKDAICVLLEGGA 758 HSP 2 Score: 66.6254 bits (161), Expect = 6.127e-11 Identity = 66/241 (27.39%), Postives = 102/241 (42.32%), Query Frame = 1
Query: 340 NRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTP---------------------------------LHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
N +++G T LH AA L +R N++A+ + Q+TPL +AA +G ++ LL+ + + TD GRTP LHLAA +GHL +VK +++ D+ ++G + L LA + + + + + E DDL P H+AA G + LL K + G T LH A GH+ VK LL++GA
Sbjct: 461 NLANAQGATPLHQAAEKRLKGVSEIL--LSRKTTNVNAKDED-QYTPLHFAAQNGDEALTRLLLDRSASINETDAQGRTPTHIACHHGQENVVRVLLSRGADVHVKGKDDWTALHLAAWKGHLGIVKLLVKQAGADV----DGQTSDGRSPLHLASQRGQYRVARIL--VELGANVHLTSDDLYAPLHVAAETGH-TSTSRLLVKHDADIKSRTANGCTALHLASQKGHLPTVKMLLAEGA 691
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Zebrafish
Match: ripk4 (receptor-interacting serine-threonine kinase 4 [Source:ZFIN;Acc:ZDB-GENE-040426-2042]) HSP 1 Score: 98.5969 bits (244), Expect = 6.998e-21 Identity = 84/268 (31.34%), Postives = 117/268 (43.66%), Query Frame = 1
Query: 259 WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRH-GVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVD-------------------------------NNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
+T H A G +A L L++ + IN D++G T H+A HG+ + + L L D + WT L AAW G + IV+LL++ G D DGR+PLHLA+ G + + +E G ++H LT+ D NG TAL LA K VK + + DP + + DL+ P H+AA NG ++ LL S N D G T LH A GH D + LL GA
Sbjct: 517 YTPLHFAAQNGDEA-LTRLLLDRSASINETDAQGRTPTHIACHHGQENVVRVL-----LSRGADVHVKGKDDWTALHLAAWKGHLGIVKLLVKQAGADVDGQTSDGRSPLHLASQRGQYRVARILVELGA-NVH--LTSDDLYAPLHVAAETGHTSTSRLLVKHDADIKSRTANGCTALHLASQKGHLPTVKMLLAEGADPES--VNHDLRTPCHLAAQNGHCEVLKELLRSCSDVANAQDRNGLTALHLAVSGGHKDAICVLLEGGA 773 HSP 2 Score: 66.6254 bits (161), Expect = 7.465e-11 Identity = 66/241 (27.39%), Postives = 102/241 (42.32%), Query Frame = 1
Query: 340 NRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTP---------------------------------LHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
N +++G T LH AA L +R N++A+ + Q+TPL +AA +G ++ LL+ + + TD GRTP LHLAA +GHL +VK +++ D+ ++G + L LA + + + + + E DDL P H+AA G + LL K + G T LH A GH+ VK LL++GA
Sbjct: 476 NLANAQGATPLHQAAEKRLKGVSEIL--LSRKTTNVNAKDED-QYTPLHFAAQNGDEALTRLLLDRSASINETDAQGRTPTHIACHHGQENVVRVLLSRGADVHVKGKDDWTALHLAAWKGHLGIVKLLVKQAGADV----DGQTSDGRSPLHLASQRGQYRVARIL--VELGANVHLTSDDLYAPLHVAAETGH-TSTSRLLVKHDADIKSRTANGCTALHLASQKGHLPTVKMLLAEGA 706
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Zebrafish
Match: ripk4 (receptor-interacting serine-threonine kinase 4 [Source:ZFIN;Acc:ZDB-GENE-040426-2042]) HSP 1 Score: 98.5969 bits (244), Expect = 7.860e-21 Identity = 84/268 (31.34%), Postives = 117/268 (43.66%), Query Frame = 1
Query: 259 WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRH-GVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVD-------------------------------NNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
+T H A G +A L L++ + IN D++G T H+A HG+ + + L L D + WT L AAW G + IV+LL++ G D DGR+PLHLA+ G + + +E G ++H LT+ D NG TAL LA K VK + + DP + + DL+ P H+AA NG ++ LL S N D G T LH A GH D + LL GA
Sbjct: 474 YTPLHFAAQNGDEA-LTRLLLDRSASINETDAQGRTPTHIACHHGQENVVRVL-----LSRGADVHVKGKDDWTALHLAAWKGHLGIVKLLVKQAGADVDGQTSDGRSPLHLASQRGQYRVARILVELGA-NVH--LTSDDLYAPLHVAAETGHTSTSRLLVKHDADIKSRTANGCTALHLASQKGHLPTVKMLLAEGADPES--VNHDLRTPCHLAAQNGHCEVLKELLRSCSDVANAQDRNGLTALHLAVSGGHKDAICVLLEGGA 730 HSP 2 Score: 66.2402 bits (160), Expect = 8.071e-11 Identity = 66/241 (27.39%), Postives = 102/241 (42.32%), Query Frame = 1
Query: 340 NRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTP---------------------------------LHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
N +++G T LH AA L +R N++A+ + Q+TPL +AA +G ++ LL+ + + TD GRTP LHLAA +GHL +VK +++ D+ ++G + L LA + + + + + E DDL P H+AA G + LL K + G T LH A GH+ VK LL++GA
Sbjct: 433 NLANAQGATPLHQAAEKRLKGVSEIL--LSRKTTNVNAKDED-QYTPLHFAAQNGDEALTRLLLDRSASINETDAQGRTPTHIACHHGQENVVRVLLSRGADVHVKGKDDWTALHLAAWKGHLGIVKLLVKQAGADV----DGQTSDGRSPLHLASQRGQYRVARIL--VELGANVHLTSDDLYAPLHVAAETGH-TSTSRLLVKHDADIKSRTANGCTALHLASQKGHLPTVKMLLAEGA 663
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Zebrafish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2]) HSP 1 Score: 92.8189 bits (229), Expect = 2.772e-19 Identity = 85/280 (30.36%), Postives = 126/280 (45.00%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLL----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
L+ LH A G +V L+D+ L + H+A G C++ LI + V + + T LH+AA HC HY ++L N +A+ N +TPL A RI ++ELL++HG + G TP+H+AA GH +V G ++ G TAL +A + +VK++ D D Q P HI++ G +Q LL+ G++ + T GYT LH A GH D+ LL GAS+ I T
Sbjct: 301 LTPLHCGARSGHEQVVEMLLDR--GAPILSKTKNGLSPLHMATQGDHLNCVQLLIHHEVPVDDVTNDY-LTALHVAA-----HCGHY--KVAKVLVDKRANPNAKALN-GFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLTNHGA-----SPNTMNVRGETALHMAARAGQANVVKFLVANGADVDAKA--KDDQTPLHISSRLGKPDIVQQLLQHGALP-DSTTTSGYTPLHLAARDGHKDVASILLDNGASLGITT 561 HSP 2 Score: 69.707 bits (169), Expect = 6.446e-12 Identity = 56/180 (31.11%), Postives = 84/180 (46.67%), Query Frame = 1
Query: 472 WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+TPL AA +G I++ LL+ G A D + TPLH+A+ G+ +V+ LE G + A +G T L +++V+ + D+ P + + L P H+ A GD LN LL + V+D N T LH A GH + K L+ K A+ K L T + I
Sbjct: 235 FTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNANMVRLLLERGAR-----IDARTKDGLTPLHCGARSGHEQVVEMLLDRGA-PILSKTKNGLS-PLHM-ATQGDHLNCVQLLIHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVLVDKRANPNAKALNGFTPLHIA 406 HSP 3 Score: 68.9366 bits (167), Expect = 9.699e-12 Identity = 60/216 (27.78%), Postives = 104/216 (48.15%), Query Frame = 1
Query: 337 INRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVNDTDNR-GYTMLHKACISGHIDIVKWLLSKGASIWIK 978
IN + G LHLA+ G + + +L +DA + T L A+ G+ +V+ L+ +G + +G TPL++AA E HL +VK+ L+ G + +GFT L +A + D++V + + + + ++ P HIAA D LL+ A D +++ G+T LH A G+I++ LL++GA++ K
Sbjct: 66 INICNQNGLNALHLASKEGH---VEVVTELIKLGATVDAATKKGN-TALHIASLAGQADVVKELVNNGANINAQSQNGFTPLYMAAQENHLDVVKFLLDNGS-----SQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGK-------VRLPALHIAARKDDTKAAALLLQNDHNA--DIESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFK 263 HSP 4 Score: 63.5438 bits (153), Expect = 5.207e-10 Identity = 65/218 (29.82%), Postives = 95/218 (43.58%), Query Frame = 1
Query: 358 GCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
G T LH+AA +G + L N + D + RN TPL A+ G ++V LL+ G D DG TPLH A GH +V+ L+ G + K NG + L +A V+ + E ++ +D H+AA G + L++K + N G+T LH AC I +++ LL GASI T T I +
Sbjct: 234 GFTPLHIAAHYGNINVATLLLNRGAAV---DFKARN-DITPLHVASKRGNANMVRLLLERGARIDARTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKT-----KNGLSPLHMATQGDHLNCVQLLIHHEVP--VDDVTNDYLTALHVAAHCGHYKVAKVLVDKRANP-NAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVA 439
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Zebrafish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2]) HSP 1 Score: 92.8189 bits (229), Expect = 2.850e-19 Identity = 85/280 (30.36%), Postives = 126/280 (45.00%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLL----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
L+ LH A G +V L+D+ L + H+A G C++ LI + V + + T LH+AA HC HY ++L N +A+ N +TPL A RI ++ELL++HG + G TP+H+AA GH +V G ++ G TAL +A + +VK++ D D Q P HI++ G +Q LL+ G++ + T GYT LH A GH D+ LL GAS+ I T
Sbjct: 273 LTPLHCGARSGHEQVVEMLLDR--GAPILSKTKNGLSPLHMATQGDHLNCVQLLIHHEVPVDDVTNDY-LTALHVAA-----HCGHY--KVAKVLVDKRANPNAKALN-GFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLTNHGA-----SPNTMNVRGETALHMAARAGQANVVKFLVANGADVDAKA--KDDQTPLHISSRLGKPDIVQQLLQHGALP-DSTTTSGYTPLHLAARDGHKDVASILLDNGASLGITT 533 HSP 2 Score: 69.3218 bits (168), Expect = 7.478e-12 Identity = 56/180 (31.11%), Postives = 84/180 (46.67%), Query Frame = 1
Query: 472 WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+TPL AA +G I++ LL+ G A D + TPLH+A+ G+ +V+ LE G + A +G T L +++V+ + D+ P + + L P H+ A GD LN LL + V+D N T LH A GH + K L+ K A+ K L T + I
Sbjct: 207 FTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNANMVRLLLERGAR-----IDARTKDGLTPLHCGARSGHEQVVEMLLDRGA-PILSKTKNGLS-PLHM-ATQGDHLNCVQLLIHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVLVDKRANPNAKALNGFTPLHIA 378 HSP 3 Score: 68.9366 bits (167), Expect = 8.902e-12 Identity = 60/216 (27.78%), Postives = 104/216 (48.15%), Query Frame = 1
Query: 337 INRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVNDTDNR-GYTMLHKACISGHIDIVKWLLSKGASIWIK 978
IN + G LHLA+ G + + +L +DA + T L A+ G+ +V+ L+ +G + +G TPL++AA E HL +VK+ L+ G + +GFT L +A + D++V + + + + ++ P HIAA D LL+ A D +++ G+T LH A G+I++ LL++GA++ K
Sbjct: 38 INICNQNGLNALHLASKEGH---VEVVTELIKLGATVDAATKKGN-TALHIASLAGQADVVKELVNNGANINAQSQNGFTPLYMAAQENHLDVVKFLLDNGS-----SQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGK-------VRLPALHIAARKDDTKAAALLLQNDHNA--DIESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFK 235 HSP 4 Score: 63.1586 bits (152), Expect = 5.349e-10 Identity = 65/218 (29.82%), Postives = 94/218 (43.12%), Query Frame = 1
Query: 358 GCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
G T LH+AA +G + L N + D + RN TPL A+ G ++V LL+ G D DG TPLH A GH +V+ L+ G + K NG + L +A V+ + E ++ +D H+AA G + L++K N G+T LH AC I +++ LL GASI T T I +
Sbjct: 206 GFTPLHIAAHYGNINVATLLLNRGAAV---DFKARN-DITPLHVASKRGNANMVRLLLERGARIDARTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKT-----KNGLSPLHMATQGDHLNCVQLLIHHEVP--VDDVTNDYLTALHVAAHCGHYKVAKVLVDK-RANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVA 411
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Xenopus
Match: rps27a (ribosomal protein S27a [Source:Xenbase;Acc:XB-GENE-5741151]) HSP 1 Score: 176.022 bits (445), Expect = 9.148e-48 Identity = 107/305 (35.08%), Postives = 165/305 (54.10%), Query Frame = 1
Query: 97 AVKDIDPVEIRKIIMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+ D DP+ + LHWAA GS+ +++L+ SD+ WTAAH+A I G+DAC+++LI+ ++ + D R C HLAAAHG L + R ++++ R W P+ YAA+HGR+ ++LL R G + D++G P HLAA EGHL +K+ + + + + L A + NG T LA KD IV+YI E + + ++L +P H+AA GDL+ ++ L+E G I +N+ D++G T LHKA G ++ ++WLL GA I ET D+
Sbjct: 13 GINDPDPLH---------KFTPLHWAAHSGSLECLHWLLWHGADVSDVTTRG--WTAAHLAAIRGQDACMQALIVNGADLSAK-DDRHCCPAHLAAAHGHSFT---LQSVLRSGVDINSSDRT-GWKPVHYAAFHGRLGCLQLLFRWGATLEDADLNGNIPAHLAAMEGHLHCLKFLVSKAS-SVTRVLEATNMNGETPKDLARKLYKDNIVQYIDGVEYERDHPDEQENLAFPAHVAAFKGDLVTLRKLVESGIININERDDKGATPLHKAAGQGQLECLQWLLEMGADYNITNEAGETPKDVA 300
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Xenopus
Match: rps27a (ribosomal protein S27a [Source:Xenbase;Acc:XB-GENE-5741151]) HSP 1 Score: 176.022 bits (445), Expect = 1.207e-47 Identity = 107/305 (35.08%), Postives = 165/305 (54.10%), Query Frame = 1
Query: 97 AVKDIDPVEIRKIIMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+ D DP+ + LHWAA GS+ +++L+ SD+ WTAAH+A I G+DAC+++LI+ ++ + D R C HLAAAHG L + R ++++ R W P+ YAA+HGR+ ++LL R G + D++G P HLAA EGHL +K+ + + + + L A + NG T LA KD IV+YI E + + ++L +P H+AA GDL+ ++ L+E G I +N+ D++G T LHKA G ++ ++WLL GA I ET D+
Sbjct: 13 GINDPDPLH---------KFTPLHWAAHSGSLECLHWLLWHGADVSDVTTRG--WTAAHLAAIRGQDACMQALIVNGADLSAK-DDRHCCPAHLAAAHGHSFT---LQSVLRSGVDINSSDRT-GWKPVHYAAFHGRLGCLQLLFRWGATLEDADLNGNIPAHLAAMEGHLHCLKFLVSKAS-SVTRVLEATNMNGETPKDLARKLYKDNIVQYIDGVEYERDHPDEQENLAFPAHVAAFKGDLVTLRKLVESGIININERDDKGATPLHKAAGQGQLECLQWLLEMGADYNITNEAGETPKDVA 300
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Xenopus
Match: rps27a (ribosomal protein S27a [Source:Xenbase;Acc:XB-GENE-5741151]) HSP 1 Score: 175.637 bits (444), Expect = 2.968e-47 Identity = 109/314 (34.71%), Postives = 170/314 (54.14%), Query Frame = 1
Query: 97 AVKDIDPVEIRKIIMEDTDL---------SLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
AV+ D ++ I+ T + + LHWAA GS+ +++L+ SD+ WTAAH+A I G+DAC+++LI+ ++ + D R C HLAAAHG L + R ++++ R W P+ YAA+HGR+ ++LL R G + D++G P HLAA EGHL +K+ + + + + L A + NG T LA KD IV+YI E + + ++L +P H+AA GDL+ ++ L+E G I +N+ D++G T LHKA G ++ ++WLL GA I ET D+
Sbjct: 25 AVRGGDVEQLAAIVQSGTGINDPDPLHKFTPLHWAAHSGSLECLHWLLWHGADVSDVTTRG--WTAAHLAAIRGQDACMQALIVNGADLSAK-DDRHCCPAHLAAAHGHSFT---LQSVLRSGVDINSSDRT-GWKPVHYAAFHGRLGCLQLLFRWGATLEDADLNGNIPAHLAAMEGHLHCLKFLVSKAS-SVTRVLEATNMNGETPKDLARKLYKDNIVQYIDGVEYERDHPDEQENLAFPAHVAAFKGDLVTLRKLVESGIININERDDKGATPLHKAAGQGQLECLQWLLEMGADYNITNEAGETPKDVA 330
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Xenopus
Match: rps27a (ribosomal protein S27a [Source:Xenbase;Acc:XB-GENE-5741151]) HSP 1 Score: 175.252 bits (443), Expect = 4.240e-47 Identity = 109/314 (34.71%), Postives = 170/314 (54.14%), Query Frame = 1
Query: 97 AVKDIDPVEIRKIIMEDTDL---------SLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
AV+ D ++ I+ T + + LHWAA GS+ +++L+ SD+ WTAAH+A I G+DAC+++LI+ ++ + D R C HLAAAHG L + R ++++ R W P+ YAA+HGR+ ++LL R G + D++G P HLAA EGHL +K+ + + + + L A + NG T LA KD IV+YI E + + ++L +P H+AA GDL+ ++ L+E G I +N+ D++G T LHKA G ++ ++WLL GA I ET D+
Sbjct: 25 AVRGGDVEQLAAIVQSGTGINDPDPLHKFTPLHWAAHSGSLECLHWLLWHGADVSDVTTRG--WTAAHLAAIRGQDACMQALIVNGADLSAK-DDRHCCPAHLAAAHGHSFT---LQSVLRSGVDINSSDRT-GWKPVHYAAFHGRLGCLQLLFRWGATLEDADLNGNIPAHLAAMEGHLHCLKFLVSKAS-SVTRVLEATNMNGETPKDLARKLYKDNIVQYIDGVEYERDHPDEQENLAFPAHVAAFKGDLVTLRKLVESGIININERDDKGATPLHKAAGQGQLECLQWLLEMGADYNITNEAGETPKDVA 330
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Xenopus
Match: rps27a (ribosomal protein S27a [Source:Xenbase;Acc:XB-GENE-5741151]) HSP 1 Score: 120.939 bits (302), Expect = 1.362e-28 Identity = 78/224 (34.82%), Postives = 120/224 (53.57%), Query Frame = 1
Query: 97 AVKDIDPVEIRKIIMEDTDL---------SLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYI 741
AV+ D ++ I+ T + + LHWAA GS+ +++L+ SD+ WTAAH+A I G+DAC+++LI+ ++ + D R C HLAAAHG L + R ++++ R W P+ YAA+HGR+ ++LL R G + D++G P HLAA EGHL +K+ + + + + L A + NG T LA KD IV+YI
Sbjct: 25 AVRGGDVEQLAAIVQSGTGINDPDPLHKFTPLHWAAHSGSLECLHWLLWHGADVSDVTTRG--WTAAHLAAIRGQDACMQALIVNGADLSAK-DDRHCCPAHLAAAHGHSFT---LQSVLRSGVDINSSDRT-GWKPVHYAAFHGRLGCLQLLFRWGATLEDADLNGNIPAHLAAMEGHLHCLKFLVSKAS-SVTRVLEATNMNGETPKDLARKLYKDNIVQYI 240 HSP 2 Score: 51.9878 bits (123), Expect = 2.312e-6 Identity = 53/178 (29.78%), Postives = 79/178 (44.38%), Query Frame = 1
Query: 469 QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGR--TPLHLAAAEGHLPLVKYFLEEGC-----HDIHKC---LTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRT-WELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
++TPL +AA G + + L+ HG DV+DV R T HLAA G ++ + G D H C L A + FT +++ D RT W+ P H AA +G L +Q L G+ + D D G H A + GH+ +K+L+SK +S+
Sbjct: 52 KFTPLHWAAHSGSLECLHWLLWHGA--DVSDVTTRGWTAAHLAAIRGQDACMQALIVNGADLSAKDDRHCCPAHLAAAHGHSFTL---------QSVLRSGVDINSSDRTGWK-------PVHYAAFHGRLGCLQLLFRWGAT-LEDADLNGNIPAHLAAMEGHLHCLKFLVSKASSV 210
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Mouse
Match: Ankrd42 (ankyrin repeat domain 42 [Source:MGI Symbol;Acc:MGI:1921095]) HSP 1 Score: 182.185 bits (461), Expect = 2.456e-51 Identity = 112/336 (33.33%), Postives = 178/336 (52.98%), Query Frame = 1
Query: 19 EYLEESEKRRLLFAYITADDDARFKRAVKDIDPVEIRKIIMEDTD----LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
E + S ++++ F+ I DA VK + + R + + D + LHWAA GS+ +++L+ WTAAH+A I G+DACL++LI+ + + D RGCT LHLAA HG + + L +D + +W P+ YA++HGR+ ++LL++ G + D +G P+HLAA EGHL +K+ L + + L A ++NG L LA+ K +V++I+ + + + DDL +PGH+AA GDL ++ L+ G I +N+ D+ G T +HKA GHID ++WL+ GA I ET D+
Sbjct: 15 ETADSSSRKKVHFSSI---HDAVRAGDVKQLSDIVERGANLNEVDALHQFTPLHWAAHSGSLECLHWLL--WSGADATQTTTRGWTAAHIAAIRGQDACLQALIINGANLATQ-DDRGCTPLHLAATHGHSFSLQIM-----LRSGVDPSVTDKREWKPVHYASFHGRLGCLQLLVKWGCGIEDVDYNGNLPVHLAAMEGHLHCLKFLLSR-MNSATQALKAFNDNGENVLDLAQRFLKQNVVEFIQGAQYEGSHPDDHDDLAFPGHVAAFKGDLEVLKKLIGDGVINLNERDDNGSTPMHKAAGQGHIDCLQWLIEMGAESNITNKAGETPSDVA 338
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Mouse
Match: Ankrd42 (ankyrin repeat domain 42 [Source:MGI Symbol;Acc:MGI:1921095]) HSP 1 Score: 181.8 bits (460), Expect = 5.373e-51 Identity = 112/336 (33.33%), Postives = 178/336 (52.98%), Query Frame = 1
Query: 19 EYLEESEKRRLLFAYITADDDARFKRAVKDIDPVEIRKIIMEDTD----LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
E + S ++++ F+ I DA VK + + R + + D + LHWAA GS+ +++L+ WTAAH+A I G+DACL++LI+ + + D RGCT LHLAA HG + + L +D + +W P+ YA++HGR+ ++LL++ G + D +G P+HLAA EGHL +K+ L + + L A ++NG L LA+ K +V++I+ + + + DDL +PGH+AA GDL ++ L+ G I +N+ D+ G T +HKA GHID ++WL+ GA I ET D+
Sbjct: 15 ETADSSSRKKVHFSSI---HDAVRAGDVKQLSDIVERGANLNEVDALHQFTPLHWAAHSGSLECLHWLL--WSGADATQTTTRGWTAAHIAAIRGQDACLQALIINGANLATQ-DDRGCTPLHLAATHGHSFSLQIM-----LRSGVDPSVTDKREWKPVHYASFHGRLGCLQLLVKWGCGIEDVDYNGNLPVHLAAMEGHLHCLKFLLSR-MNSATQALKAFNDNGENVLDLAQRFLKQNVVEFIQGAQYEGSHPDDHDDLAFPGHVAAFKGDLEVLKKLIGDGVINLNERDDNGSTPMHKAAGQGHIDCLQWLIEMGAESNITNKAGETPSDVA 338
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Mouse
Match: Ankrd42 (ankyrin repeat domain 42 [Source:MGI Symbol;Acc:MGI:1921095]) HSP 1 Score: 185.267 bits (469), Expect = 9.361e-51 Identity = 112/336 (33.33%), Postives = 178/336 (52.98%), Query Frame = 1
Query: 19 EYLEESEKRRLLFAYITADDDARFKRAVKDIDPVEIRKIIMEDTD----LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
E + S ++++ F+ I DA VK + + R + + D + LHWAA GS+ +++L+ WTAAH+A I G+DACL++LI+ + + D RGCT LHLAA HG + + L +D + +W P+ YA++HGR+ ++LL++ G + D +G P+HLAA EGHL +K+ L + + L A ++NG L LA+ K +V++I+ + + + DDL +PGH+AA GDL ++ L+ G I +N+ D+ G T +HKA GHID ++WL+ GA I ET D+
Sbjct: 15 ETADSSSRKKVHFSSI---HDAVRAGDVKQLSDIVERGANLNEVDALHQFTPLHWAAHSGSLECLHWLL--WSGADATQTTTRGWTAAHIAAIRGQDACLQALIINGANLATQ-DDRGCTPLHLAATHGHSFSLQIM-----LRSGVDPSVTDKREWKPVHYASFHGRLGCLQLLVKWGCGIEDVDYNGNLPVHLAAMEGHLHCLKFLLSR-MNSATQALKAFNDNGENVLDLAQRFLKQNVVEFIQGAQYEGSHPDDHDDLAFPGHVAAFKGDLEVLKKLIGDGVINLNERDDNGSTPMHKAAGQGHIDCLQWLIEMGAESNITNKAGETPSDVA 338
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Mouse
Match: Ankrd42 (ankyrin repeat domain 42 [Source:MGI Symbol;Acc:MGI:1921095]) HSP 1 Score: 185.267 bits (469), Expect = 1.104e-50 Identity = 112/336 (33.33%), Postives = 178/336 (52.98%), Query Frame = 1
Query: 19 EYLEESEKRRLLFAYITADDDARFKRAVKDIDPVEIRKIIMEDTD----LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
E + S ++++ F+ I DA VK + + R + + D + LHWAA GS+ +++L+ WTAAH+A I G+DACL++LI+ + + D RGCT LHLAA HG + + L +D + +W P+ YA++HGR+ ++LL++ G + D +G P+HLAA EGHL +K+ L + + L A ++NG L LA+ K +V++I+ + + + DDL +PGH+AA GDL ++ L+ G I +N+ D+ G T +HKA GHID ++WL+ GA I ET D+
Sbjct: 15 ETADSSSRKKVHFSSI---HDAVRAGDVKQLSDIVERGANLNEVDALHQFTPLHWAAHSGSLECLHWLL--WSGADATQTTTRGWTAAHIAAIRGQDACLQALIINGANLATQ-DDRGCTPLHLAATHGHSFSLQIM-----LRSGVDPSVTDKREWKPVHYASFHGRLGCLQLLVKWGCGIEDVDYNGNLPVHLAAMEGHLHCLKFLLSR-MNSATQALKAFNDNGENVLDLAQRFLKQNVVEFIQGAQYEGSHPDDHDDLAFPGHVAAFKGDLEVLKKLIGDGVINLNERDDNGSTPMHKAAGQGHIDCLQWLIEMGAESNITNKAGETPSDVA 338
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Mouse
Match: Ankrd44 (ankyrin repeat domain 44 [Source:MGI Symbol;Acc:MGI:3045243]) HSP 1 Score: 93.5893 bits (231), Expect = 2.456e-19 Identity = 90/294 (30.61%), Postives = 137/294 (46.60%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDN-NAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAA--HGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKC---------LTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG----HIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI-----WIKT 981
LH AA G + +V L++ ++D N TA H+A G+DA + LI V N+P++ G T LH AAA HG C+ L N +++ Q ++ + +PL A HGR + + LI++G D D DG TPLH+AA GH L+ + G D KC L A++ + KL + K IV ++ +E+ D G H AA G++ I+ LL+ + D G T LH A + H +K L++ GA++ W +T
Sbjct: 7 LHAAASNGQISVVKHLLN---LGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGANV-NQPNNSGFTPLHFAAASTHGAL-CLELLVNNG---ADVNIQSKDGK-SPLHMTAVHGRFTRSQTLIQNGGEIDCVDKDGNTPLHVAARHGHELLINTLITSGA-DTAKCGIHSMFPLHLAALNAHSDCCRKLLSSGQKYSIVSLFSNEHVLSAGFEI-DTPDTFGRTCLHAAAAGGNVECIK-LLQSSGADFHKKDKCGRTPLHYAAANCHFHCIKALVTTGANVNETDDWGRT 288 HSP 2 Score: 71.633 bits (174), Expect = 1.968e-12 Identity = 79/278 (28.42%), Postives = 125/278 (44.96%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHG---VATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKE-----KDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVND-----TDNRGYTMLHKACISGHIDIVKWLLSK 957
+H+AA G + L+++ + + + + H+A G LE L+ ++ R D +G T L+LAA G C+ L N + D + TPL + +G + LL+ DV D G+TPL LA A GH+ V LE+ + + AVD G TAL +E V+ + ++E KD R + P H AA G + LL+ IA+++ DN+GYT LH AC +G+ + ++ LL +
Sbjct: 351 IHYAAAYGHRQCLELLLERTNTGFEESDGGALKSPLHLAAYNGHHQALEVLLQSLVDLDIR-DEKGRTALYLAAFKGHTECVEALVNQGASIFVKD---NVTKRTPLHASVINGHTLCLRLLLETADNPEVVDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEAN-----VDAVDIVGCTALHRGIMTGHEECVQMLLEQEASILCKDSRG-------RTPLHYAAARGHATWLNELLQ---IALSEEDCCLKDNQGYTPLHWACYNGNENCIEVLLEQ 609 HSP 3 Score: 69.707 bits (169), Expect = 8.014e-12 Identity = 79/286 (27.62%), Postives = 123/286 (43.01%), Query Frame = 1
Query: 136 IMEDTDLSLLHWAALKGSVVIVNFLIDKME--FPSDLDNNAEIWTAAHVAVIGGKDACLESLI--MRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQY-PGHIAAVNGDLLNIQGLLEKGSI---AVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
I ++ + L+ AA KG V L+++ F D N T H +VI G CL L+ PEV++ D++G T L LA A+G + L + +D + T L G V++L+ + D GRTPLH AAA GH + L+ + CL DN G+T L A + ++ + ++ + + F + P H A +NG LL G+I V+ D++G T LH A H + ++ LL A +
Sbjct: 410 IRDEKGRTALYLAAFKGHTECVEALVNQGASIFVKD---NVTKRTPLHASVINGHTLCLRLLLETADNPEVVDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEANVDAVDI----VGCTALHRGIMTGHEECVQMLLEQEASILCKDSRGRTPLHYAAARGHATWLNELLQIALSEEDCCLK--DNQGYTPLHWACYNGNENCIEVLLEQ----KCFRKFIGNPFTPLHCAIINGHESCASLLL--GAIDPSIVSCRDDKGRTTLHAAAFGDHAECLQLLLRHDAQV 680 HSP 4 Score: 67.781 bits (164), Expect = 2.745e-11 Identity = 76/261 (29.12%), Postives = 114/261 (43.68%), Query Frame = 1
Query: 232 SDLDNNAEIWTAAH--------VAVIGGKDA--CLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDN-NGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFD-DLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIK 978
SD+D N I AH + KDA CLE L+ R D G +H AAA+G C+ L R + +PL AA++G +E+L++ V D+ D GRT L+LAA +GH V+ + +G K DN T L + ++ + + +P ++ D Q P +A G + + LLEK V+ D G T LH+ ++GH + V+ LL + ASI K
Sbjct: 296 SDMDRNKMILGNAHDNSEELERAREVKEKDAALCLEFLLQNDANPSIR-DKEGYNSIHYAAAYGHRQCLELL--LERTNTGFEESDGGALKSPLHLAAYNGHHQALEVLLQSLVDLDIRDEKGRTALYLAAFKGHTECVEALVNQGASIFVK-----DNVTKRTPLHASVINGHTLCLRLLLETADNPEVVDVKDAKGQTPLMLAVAYGHIDAVSLLLEK-EANVDAVDIVGCTALHRGIMTGHEECVQMLLEQEASILCK 547 HSP 5 Score: 65.855 bits (159), Expect = 1.371e-10 Identity = 49/165 (29.70%), Postives = 81/165 (49.09%), Query Frame = 1
Query: 472 WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEI-VKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
+TPL AA +G+IS+V+ L+ GV D +V G T LH+A G +V ++ G + + +N+GFT L A + + ++ + + D D + P H+ AV+G Q L++ G ++ D G T LH A GH ++ L++ GA
Sbjct: 4 YTPLHAAASNGQISVVKHLLNLGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGAN-----VNQPNNSGFTPLHFAAASTHGALCLELLVNNGADVNIQS--KDGKSPLHMTAVHGRFTRSQTLIQNGG-EIDCVDKDGNTPLHVAARHGHELLINTLITSGA 160 HSP 6 Score: 65.0846 bits (157), Expect = 2.398e-10 Identity = 71/240 (29.58%), Postives = 106/240 (44.17%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDL---DNNAEIWTAAHVAVIGGKDACLESLIMRKP--EVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHG-RISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDD-LQYPGHIAAVNGDLLNIQGLLEKGS 861
LH+AA +G +N L+ D DN +T H A G + C+E L+ +K + I P T LH A +G C L + P++ CR+ + ++AA G ++LL+RH + D G+T L +AA G V + D LT D + T L LA +K ++ I DK +D + LQ P HIAA NG + ++ LL KG+
Sbjct: 555 LHYAAARGHATWLNELLQIALSEEDCCLKDNQG--YTPLHWACYNGNENCIEVLLEQKCFRKFIGNP----FTPLHCAIINGHESCASLL--LGAIDPSI-VSCRDDKGRTTLHAAAFGDHAECLQLLLRHDAQVNAVDNSGKTALMMAAENGQAGAVDILVNSAQAD----LTVKDKDLNTPLHLAISKGHEKCALLILDKIQDESLINAKNSALQTPLHIAARNGLKVVVEELLAKGA 781 HSP 7 Score: 62.7734 bits (151), Expect = 1.260e-9 Identity = 65/239 (27.20%), Postives = 103/239 (43.10%), Query Frame = 1
Query: 337 INRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHK---------------CL----------TAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIK 978
I+ PD+ G T LH AAA G C+ L + D R TPL YAA + ++ L+ G + TD GRT LH AAA + K L HD + CL + D G+ ++ A + ++ + ++ L+ P H+AA NG ++ LL+ + ++ D +G T L+ A GH + V+ L+++GASI++K
Sbjct: 213 IDTPDTFGRTCLHAAAAGGNVECIKLLQSSGADFHKKDKCGR----TPLHYAAANCHFHCIKALVTTGANVNETDDWGRTALHYAAASD-MDRNKMILGNA-HDNSEELERAREVKEKDAALCLEFLLQNDANPSIRDKEGYNSIHYAAAYGHRQCLELLLERTNTGFEESDGGALKSPLHLAAYNGHHQALEVLLQS-LVDLDIRDEKGRTALYLAAFKGHTECVEALVNQGASIFVK 444
BLAST of Alpha-latrocrustotoxin-Lt1a vs. UniProt/SwissProt
Match: sp|Q3V096|ANR42_MOUSE (Ankyrin repeat domain-containing protein 42 OS=Mus musculus OX=10090 GN=Ankrd42 PE=1 SV=1) HSP 1 Score: 185.267 bits (469), Expect = 7.724e-50 Identity = 112/336 (33.33%), Postives = 178/336 (52.98%), Query Frame = 1
Query: 19 EYLEESEKRRLLFAYITADDDARFKRAVKDIDPVEIRKIIMEDTD----LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
E + S ++++ F+ I DA VK + + R + + D + LHWAA GS+ +++L+ WTAAH+A I G+DACL++LI+ + + D RGCT LHLAA HG + + L +D + +W P+ YA++HGR+ ++LL++ G + D +G P+HLAA EGHL +K+ L + + L A ++NG L LA+ K +V++I+ + + + DDL +PGH+AA GDL ++ L+ G I +N+ D+ G T +HKA GHID ++WL+ GA I ET D+
Sbjct: 15 ETADSSSRKKVHFSSI---HDAVRAGDVKQLSDIVERGANLNEVDALHQFTPLHWAAHSGSLECLHWLL--WSGADATQTTTRGWTAAHIAAIRGQDACLQALIINGANLATQ-DDRGCTPLHLAATHGHSFSLQIM-----LRSGVDPSVTDKREWKPVHYASFHGRLGCLQLLVKWGCGIEDVDYNGNLPVHLAAMEGHLHCLKFLLSR-MNSATQALKAFNDNGENVLDLAQRFLKQNVVEFIQGAQYEGSHPDDHDDLAFPGHVAAFKGDLEVLKKLIGDGVINLNERDDNGSTPMHKAAGQGHIDCLQWLIEMGAESNITNKAGETPSDVA 338
BLAST of Alpha-latrocrustotoxin-Lt1a vs. UniProt/SwissProt
Match: sp|Q8N9B4|ANR42_HUMAN (Ankyrin repeat domain-containing protein 42 OS=Homo sapiens OX=9606 GN=ANKRD42 PE=2 SV=2) HSP 1 Score: 164.851 bits (416), Expect = 1.192e-43 Identity = 93/252 (36.90%), Postives = 142/252 (56.35%), Query Frame = 1
Query: 259 WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
WTA+H+A I G+DAC+++LIM + + D RGCT LHLAA HG + + L +D + +W P+ YAA+HGR+ ++LL++ G + + D +G P+HLAA EGHL K FL + L A ++NG L LA+ K I+++I+ E + + E + L +PGH+AA GDL ++ L+E G I +N+ + G T +HKA GHI+ ++WL+ GA I E D+
Sbjct: 66 WTASHIAAIRGQDACVQALIMNGANLTAQ-DDRGCTPLHLAATHGHSFTLQIM-----LRSGVDPSVTDKREWRPVHYAAFHGRLGCLQLLVKWGCSIEDVDYNGNLPVHLAAMEGHLHCFK-FLVSRMSSATQVLKAFNDNGENVLDLAQRFFKQNILQFIQGAEYEGKDLEDQETLAFPGHVAAFKGDLGMLKKLVEDGVININERADNGSTPMHKAAGQGHIECLQWLIKMGADSNITNKAGERPSDVA 310
BLAST of Alpha-latrocrustotoxin-Lt1a vs. UniProt/SwissProt
Match: sp|Q54KA7|SECG_DICDI (Ankyrin repeat, PH and SEC7 domain containing protein secG OS=Dictyostelium discoideum OX=44689 GN=secG PE=2 SV=1) HSP 1 Score: 95.5153 bits (236), Expect = 5.485e-19 Identity = 90/304 (29.61%), Postives = 130/304 (42.76%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDK--MEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRK--PEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEG----CHDI-------HKCLTA-----------------VDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWI 975
L WA+ +G + + L++K ++ + D N T H A + C+ L+ K P + + G T LH A+A G C+ L + +D C TPL A++ G S V LL++ G D D+ G +PLH AA+ G++ V+ + G C DI H C VD G T L A E+ +Y+ DP + D Q H+AA NG LL++ LL + +N D G T LHKA +GH K L+ KGA I I
Sbjct: 106 LQWASSRGHLECIKLLVEKGGVDVNTKDDKNG---TPLHKASLFASAECVLYLLNGKADPRAVT---TNGETPLHHASAGGNPQCVELLIKADSKVNAVDNDC----ITPLHQASFSGHSSCVSLLLKKGAKVDPRDIHGISPLHNAASAGYVDCVEQLVRNGENINCVDIEGVTPLHHTCFNGNLQLTKRLIELGAKINMVDEMGETPLHKAAFNGHKEVCEYLL--YLDPTMIDCRDSRQSTSLHLAAFNG-LLDMVDLLIRYKAQINIKDEEGATPLHKASFNGHSSCAKLLVDKGAPICI 396 HSP 2 Score: 84.7297 bits (208), Expect = 1.361e-15 Identity = 58/165 (35.15%), Postives = 84/165 (50.91%), Query Frame = 1
Query: 475 TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
TPL +AA+ G + V L+ ++ D G TPL A++ GHL +K +E+G D++ T D NG T L A + E V Y+ + + DPR + + + P H A+ G+ ++ LL K VN DN T LH+A SGH V LL KGA +
Sbjct: 71 TPLHHAAFCGSAACVNFLLDKKANANIKDSAGNTPLQWASSRGHLECIKLLVEKGGVDVN---TKDDKNG-TPLHKASLFASAECVLYLLNGKADPRA--VTTNGETPLHHASAGGNPQCVE-LLIKADSKVNAVDNDCITPLHQASFSGHSSCVSLLLKKGAKV 228 HSP 3 Score: 77.0258 bits (188), Expect = 3.491e-13 Identity = 96/306 (31.37%), Postives = 133/306 (43.46%), Query Frame = 1
Query: 88 FKRAVKDIDPVEIRKIIMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDE-IVKYIRDKEKDPRTWELFDDL-QYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQET 996
K+ K +DP +I I S LH AA G V V L+ E + +D E T H G + LI + IN D G T LH AA +G YL L P + CR+ Q T L AA++G + +V+LLIR+ ++ D +G TPLH A+ GH K +++G + VD+ G T L A + + + IR + E+ D P H AA NG + LL+KG+ VN D T LH A +G D V L+ A I K +T
Sbjct: 222 LKKGAK-VDPRDIHGI-------SPLHNAASAGYVDCVEQLVRNGENINCVD--IEGVTPLHHTCFNGNLQLTKRLIELGAK-INMVDEMGETPLHKAAFNGHKEVCEYL---LYLDPTM-IDCRDSRQSTSLHLAAFNGLLDMVDLLIRYKAQINIKDEEGATPLHKASFNGHSSCAKLLVDKGAP-----ICIVDSQGATPLHKAAFNGRSKCLATLIRSGAE----LEVKDSQGGTPLHNAAYNGHSDCCRILLKKGA-NVNAVDTHSSTPLHLASAAGARDTVDVLIQFKARIDAKNFAGKT 502 HSP 4 Score: 58.151 bits (139), Expect = 2.206e-7 Identity = 57/207 (27.54%), Postives = 89/207 (43.00%), Query Frame = 1
Query: 136 IMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCH-DIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKE 753
I ++ + LH A+ G L+DK +D ++ T H A G+ CL +LI E + DS+G T LH AA +G C L + +D TPL A+ G V++LI+ D + G+TPL A + H + + + G D ++VD FT K T++ DEI + + +E
Sbjct: 363 IKDEEGATPLHKASFNGHSSCAKLLVDKGAPICIVD--SQGATPLHKAAFNGRSKCLATLIRSGAE-LEVKDSQGGTPLHNAAYNGHSDCCRILLKKGANVNAVDTHSS----TPLHLASAAGARDTVDVLIQFKARIDAKNFAGKTPLVYAIKKNHSDVARVLIRAGADLDQVSLRSSVD---FT--KTFGTENHDEIYQIVNKRE 557
BLAST of Alpha-latrocrustotoxin-Lt1a vs. UniProt/SwissProt
Match: sp|Q5F478|ANR44_CHICK (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B OS=Gallus gallus OX=9031 GN=ANKRD44 PE=2 SV=1) HSP 1 Score: 94.7449 bits (234), Expect = 1.081e-18 Identity = 90/286 (31.47%), Postives = 133/286 (46.50%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFL------IDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAA--HGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI-----WIKT 981
LH AA G + IV L ID+M + TA H+A G+D+ + LI V N+P++ G T LH AAA HG C+ L N +++ Q ++ + +PL A HGR + + LI++G D D DG TPLH+AA GH L+ + G D KC +N F L LA + + + + + T + F H AA G++ I+ LL+ N D RG T LH A + H ++ L++ GA+I W +T
Sbjct: 210 LHAAASNGQINIVKHLLNLGVEIDEMNIYGN--------TALHIACYNGQDSVVNELIDYGANV-NQPNNNGFTPLHFAAASTHGAL-CLELLVNNG---ADVNIQSKDGK-SPLHMTAVHGRFTRSQTLIQNGGEIDCVDKDGNTPLHVAARYGHELLINTLITSGA-DTAKCGI---HNMFP-LHLAALNAHSDCCRKLLSSGFEIDTPDSFGRTCL--HAAAAGGNVECIK-LLQSSGADFNKKDKRGRTPLHYAAANCHFHCIETLVTTGANINETDDWGRT 473 HSP 2 Score: 78.1814 bits (191), Expect = 1.430e-13 Identity = 81/284 (28.52%), Postives = 127/284 (44.72%), Query Frame = 1
Query: 136 IMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKE-----KDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVND-----TDNRGYTMLHKACISGHIDIVKWLLSK 957
I + + +H+AA G + L++K + +++ + H+A G LE L+ + ++ D +G T L LAA G C+ L + + D + TPL + +G + LL+ DVTD G+TPL LA A GH+ V LE+ + A D G TAL +E V+ + +KE KD R + P H AA G + LL+ IA+++ DN+GYT LH AC +GH + ++ LL +
Sbjct: 527 IQDKEGYNTVHYAAAYGHRQCLELLLEKNSNMFEESDSSATKSPLHLAAYNGHHQALEVLLQSLVD-LDIKDEKGRTALDLAAFKGHAECVEALISQGASVTVKD---NVTKRTPLHASVINGHTPCLRLLLEVADNPDVTDAKGQTPLMLAVAYGHIDAVSLLLEK-----EASVDAADLLGCTALHRGIMTGHEECVQMLLEKEVSILCKDARG-------RTPLHFAAARGHATWLSELLQ---IALSEEDCSLKDNQGYTPLHWACYNGHENCIEVLLEQ 791 HSP 3 Score: 75.0998 bits (183), Expect = 1.281e-12 Identity = 93/371 (25.07%), Postives = 143/371 (38.54%), Query Frame = 1
Query: 55 FAYITADDDARFKRAVKDIDPVEIRKIIMEDTDLSLL--------HWAALKGSVVIVNFLI---------DKM---------------------EFPSDLDNNAEIW-TAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEG-----------------CH-----------DIHKCLTAVDNNGFTALKLAETKSKDEI-VKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
A + D +A+ DP EIR +I + D++ L H A+ G I+ LI D M + +D++ + W T HVA C E +I+ +N D G T LH AA +G ++ L + D + R L +AA+ G + +V LLI HG D G TPLH AA+ G + +VK+ L G C+ D + +NNGFT L A + + ++ + + D D + P H+ AV+G Q L++ G ++ D G T LH A GH ++ L++ GA
Sbjct: 1 MAVLKLADQPPLVQAIFSGDPEEIRMLIYKTEDVNALDAEKRTPLHVASFLGDADIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKALKCAE-IIIPMLSSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRR----ALHWAAYMGHLEVVALLINHGAEVTCKDKKGYTPLHAAASNGQINIVKHLLNLGVEIDEMNIYGNTALHIACYNGQDSVVNELIDYGANVNQPNNNGFTPLHFAAASTHGALCLELLVNNGADVNIQS--KDGKSPLHMTAVHGRFTRSQTLIQNGG-EIDCVDKDGNTPLHVAARYGHELLINTLITSGA 363 HSP 4 Score: 73.1738 bits (178), Expect = 5.336e-12 Identity = 83/301 (27.57%), Postives = 125/301 (41.53%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAA---------------------------EGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDD--LQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIK 978
LH AA G +++N LI + H+A + C L+ E I+ PDS G T LH AAA G C+ L + D + R TPL YAA + +E L+ G + TD GRTPLH AAA E L FL + +D + + D G+ + A + ++ + EK+ +E D + P H+AA NG ++ LL+ + ++ D +G T L A GH + V+ L+S+GAS+ +K
Sbjct: 343 LHVAARYGHELLINTLITSGADTAKC--GIHNMFPLHLAALNAHSDCCRKLLSSGFE-IDTPDSFGRTCLHAAAAGGNVECIKLLQSSGADFNKKDKRGR----TPLHYAAANCHFHCIETLVTTGANINETDDWGRTPLHYAAASDMDRKKNILGNSHENAEELERTSEMKEKEAALCLEFLLQ--NDANPSIQ--DKEGYNTVHYAAAYGHRQCLELLL--EKNSNMFEESDSSATKSPLHLAAYNGHHQALEVLLQS-LVDLDIKDEKGRTALDLAAFKGHAECVEALISQGASVTVK 629 HSP 5 Score: 71.2478 bits (173), Expect = 1.838e-11 Identity = 81/289 (28.03%), Postives = 125/289 (43.25%), Query Frame = 1
Query: 136 IMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPD---SRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFL-----EEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSI---AVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
I ++ + L AA KG V LI + + + +N T H +VI G CL L+ EV + PD ++G T L LA A+G + L + D + T L G V++L+ V+ D GRTPLH AAA GH + L EE C + DN G+T L A + ++ + +++ + + + P H A +N D N +L G+I VN D++G T LH A + H++ ++ LLS A +
Sbjct: 595 IKDEKGRTALDLAAFKGHAECVEALISQGASVT-VKDNVTKRTPLHASVINGHTPCLRLLL----EVADNPDVTDAKGQTPLMLAVAYGHIDAVSLLLEKEASVDAADL----LGCTALHRGIMTGHEECVQMLLEKEVSILCKDARGRTPLHFAAARGHATWLSELLQIALSEEDC-------SLKDNQGYTPLHWACYNGHENCIEVLLEQK---FFRKFYGNSFSPLHCAVIN-DHENCASML-IGAIDASIVNCKDDKGRTPLHAAAFADHVECLQLLLSHSAQV 862 HSP 6 Score: 67.781 bits (164), Expect = 2.674e-10 Identity = 71/251 (28.29%), Postives = 118/251 (47.01%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFL--IDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQ----WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDD-LQYPGHIAAVNGDLLNIQGLLEKGS--IAVNDTDNR 888
LH+AA +G ++ L I E L +N + +T H A G + C+E L+ +K + + LH A + +C + L+ +DA N + TPL AA+ + ++LL+ H + D G+T L +AA GH+ V + + D LT D + T+L LA +K ++ I DK ++ ++ LQ P HIAA NG + ++ LL KG+ +AV++ +R
Sbjct: 737 LHFAAARGHATWLSELLQIALSEEDCSLKDN-QGYTPLHWACYNGHENCIEVLLEQK--FFRKFYGNSFSPLHCAVINDHENCA------SMLIGAIDASIVNCKDDKGRTPLHAAAFADHVECLQLLLSHSAQVNAVDHAGKTALMMAAQNGHVGAVDFLVNIAKAD----LTLRDKDSNTSLHLASSKGHEKCALLILDKIQEQSLINAKNNSLQTPLHIAARNGLKMVVEELLAKGACVLAVDENVSR 974 HSP 7 Score: 65.855 bits (159), Expect = 1.054e-9 Identity = 65/230 (28.26%), Postives = 103/230 (44.78%), Query Frame = 1
Query: 301 CLESLIMR--KPEVINRPDSRGCTLLHLAAAHGRYHCMHYL-ANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDN-NGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIK 978
CLE L+ P + D G +H AAA+G C+ L + + D+ +PL AA++G +E+L++ V D+ D GRT L LAA +GH V+ + +G +T DN T L + ++ + + +P + Q P +A G + + LLEK +V+ D G T LH+ ++GH + V+ LL K SI K
Sbjct: 514 CLEFLLQNDANPSI---QDKEGYNTVHYAAAYGHRQCLELLLEKNSNMFEESDSSATK---SPLHLAAYNGHHQALEVLLQSLVDLDIKDEKGRTALDLAAFKGHAECVEALISQG-----ASVTVKDNVTKRTPLHASVINGHTPCLRLLLEVADNPDVTDAKG--QTPLMLAVAYGHIDAVSLLLEK-EASVDAADLLGCTALHRGIMTGHEECVQMLLEKEVSILCK 729 HSP 8 Score: 62.7734 bits (151), Expect = 7.901e-9 Identity = 50/168 (29.76%), Postives = 81/168 (48.21%), Query Frame = 1
Query: 478 PLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDE----IVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
PL+ A + G + +LI + D + RTPLH+A+ G +++ + G + A DN T L A +E ++K+ D + W Q P H+AA N L + ++ S +VN +D G T LH A ++GH+++V LL+KGA+I
Sbjct: 11 PLVQAIFSGDPEEIRMLIYKTEDVNALDAEKRTPLHVASFLGDADIIELLILSGAR-----VNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNW------QTPLHVAAANKALKCAEIIIPMLS-SVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANI 166 HSP 9 Score: 60.8474 bits (146), Expect = 3.388e-8 Identity = 63/220 (28.64%), Postives = 98/220 (44.55%), Query Frame = 1
Query: 313 LIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQW-TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
+++ K E +N D+ T LH+A+ G + L +L ++ W TPL A V++LI+H + D + +TPLH+AAA L + + + + D G TAL A E+V + K + ++ D + H AA G L + L+ G+ V D +GYT LH A +G I+IVK LL+ G I
Sbjct: 26 MLIYKTEDVNALDAEKRTPLHVASFLGDADIIELL-----ILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKALKCAEIIIP-----MLSSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKD--RRALHWAAYMGHLEVVALLINHGA-EVTCKDKKGYTPLHAAASNGQINIVKHLLNLGVEI 232
BLAST of Alpha-latrocrustotoxin-Lt1a vs. UniProt/SwissProt
Match: sp|Q5RF15|TNI3K_PONAB (Serine/threonine-protein kinase TNNI3K OS=Pongo abelii OX=9601 GN=TNNI3K PE=2 SV=3) HSP 1 Score: 91.2781 bits (225), Expect = 6.925e-18 Identity = 90/315 (28.57%), Postives = 133/315 (42.22%), Query Frame = 1
Query: 145 DTDLSLLHWAAL-KGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCH-DIHKCLTAVDNNGFTALKLAETKSKDEIVKYI--RDKEKDPRTWELFDDLQYPGHIAAVNG------DLLNIQG------------------------------LLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
+ LSLLH + G+ + L+ K PS L N +TA H+AV + SL+ + I + G T LH+A G L + DA + +TPL AA++G + LL++ G +V+ G PLHLA+A+G L + K +EEG D+ A DN L +IVKY+ D E P ++ D P H+A NG +++ I G LL++ I +N G+T LH AC GHI +V++LL GA +
Sbjct: 65 ENGLSLLHLCCICGGNKSHIRTLMLKGLRPSRLTRNG--FTALHLAVYKDNAELITSLLHGGAD-IQQVGYGGLTALHIATIAGHLEAADVLLQHGANVNIQDA----VFFTPLHIAAYYGHEQVTRLLLKFGADVNVSGEVGDRPLHLASAKGFLNIAKLLMEEGSKADV----NAQDNEDHVPLHFCSRFGHHDIVKYLLQNDLEVQPHVVNIYGDT--PLHLACYNGKFEVAKEIIQISGTESLTKENIFSETAFHSACTYGKSIDLVKFLLDQNVININHQGRDGHTGLHSACYHGHIHLVQFLLDNGADM 366
BLAST of Alpha-latrocrustotoxin-Lt1a vs. TrEMBL
Match: A0A3M6T632 (ANK_REP_REGION domain-containing protein OS=Pocillopora damicornis OX=46731 GN=pdam_00000898 PE=4 SV=1) HSP 1 Score: 196.438 bits (498), Expect = 1.017e-51 Identity = 113/319 (35.42%), Postives = 177/319 (55.49%), Query Frame = 1
Query: 82 ARFKRAVKDIDPVEIRKII-----MEDTD----LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
A AV+ D +E++ ++ + D D + LHWAA GS+ +++L+ D + WT +H+A I G+DACL++L + +N D+RGCT HLAAAHG +C+ + + D NM WTP+ AA+HGR+ ++LL R G + D D G TP HLAA+EGHLP +K+ + G I + A ++ G T LA+ K + + Y+ E D E ++L +P H+AA NGDL ++ L+E G ++VN+ D++G T HKA +GH+ +++WL+ GA++ I ET D+
Sbjct: 8 ANIHEAVRYGDVLELQSMVQRGAGINDADGKFKFTPLHWAAHHGSLECLHWLL--WHGADHSDKTPQGWTPSHLAAIRGQDACLQALAANGAD-LNDKDNRGCTAAHLAAAHGNSYCLQSILRHGVDINATD----NMGWTPVHCAAFHGRLGCLQLLSRWGASVDEVDHSGSTPAHLAASEGHLPCLKFIVCAGA-SIDHTMNARNDQGDTPKNLAQQFYKKDCIDYLNAVEYDLLHPEDEENLAFPAHVAAYNGDLAQLKMLIETGVVSVNERDDKGSTPAHKAAGNGHVQVLQWLVENGANLRIVNSAGETPKDVA 318
BLAST of Alpha-latrocrustotoxin-Lt1a vs. TrEMBL
Match: A0A5F8GMZ4 (Ankyrin repeat domain 42 OS=Monodelphis domestica OX=13616 GN=ANKRD42 PE=4 SV=1) HSP 1 Score: 190.66 bits (483), Expect = 9.602e-50 Identity = 105/283 (37.10%), Postives = 167/283 (59.01%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIR--DKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVID 1005
LHWAA GS+ +++L+ + + WTAAH+A I G+DAC+++LIM + + PD RGC+ HLAA HG + + + + + PN+ +C W P+ YA++HGR+ ++LL++ G A + TD +G P+HLAA EGHL K+ L + + L ++NG A+ LA+ KD I+++I+ D E D R+ + + L +PGH AA GDL ++ L+E G I +N+ D+ T+LHKA GH++ ++WL+ KGA + I E +D
Sbjct: 49 LHWAAHSGSLECLHWLL--WHGADIMSVSPGGWTAAHIAAIKGQDACMQALIMNGAD-LTLPDDRGCSPSHLAATHGHSYTLQVMLR-SGVDPNVSDKC---DWKPVHYASFHGRLGCLQLLVKWGAAIEDTDQNGNLPVHLAAMEGHLYCFKFLLSR-MSSVRQTLKVFNDNGENAMDLAQRFFKDNIIQFIQGTDFEGDNRSDQ--ETLAFPGHAAAYKGDLEMLKKLVENGIININERDDSESTLLHKAAGQGHVECLQWLIEKGADVNITNKAGELAVD 321 HSP 2 Score: 62.3882 bits (150), Expect = 2.175e-6 Identity = 49/167 (29.34%), Postives = 77/167 (46.11%), Query Frame = 1
Query: 469 QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
++TPL +AA G + + L+ HG G T H+AA +G ++ + G LT D+ G + LA T ++ + DP + D P H A+ +G L +Q LL K A+ DTD G +H A + GH+ K+LLS+ +S+
Sbjct: 45 KFTPLHWAAHSGSLECLHWLLWHGADIMSVSPGGWTAAHIAAIKGQDACMQALIMNGAD-----LTLPDDRGCSPSHLAATHGHSYTLQVMLRSGVDPNVSDKCD--WKPVHYASFHGRLGCLQ-LLVKWGAAIEDTDQNGNLPVHLAAMEGHLYCFKFLLSRMSSV 203
BLAST of Alpha-latrocrustotoxin-Lt1a vs. TrEMBL
Match: F6VRI7 (Ankyrin repeat domain 42 OS=Monodelphis domestica OX=13616 GN=ANKRD42 PE=4 SV=2) HSP 1 Score: 191.045 bits (484), Expect = 1.289e-49 Identity = 105/283 (37.10%), Postives = 167/283 (59.01%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIR--DKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVID 1005
LHWAA GS+ +++L+ + + WTAAH+A I G+DAC+++LIM + + PD RGC+ HLAA HG + + + + + PN+ +C W P+ YA++HGR+ ++LL++ G A + TD +G P+HLAA EGHL K+ L + + L ++NG A+ LA+ KD I+++I+ D E D R+ + + L +PGH AA GDL ++ L+E G I +N+ D+ T+LHKA GH++ ++WL+ KGA + I E +D
Sbjct: 49 LHWAAHSGSLECLHWLL--WHGADIMSVSPGGWTAAHIAAIKGQDACMQALIMNGAD-LTLPDDRGCSPSHLAATHGHSYTLQVMLR-SGVDPNVSDKC---DWKPVHYASFHGRLGCLQLLVKWGAAIEDTDQNGNLPVHLAAMEGHLYCFKFLLSR-MSSVRQTLKVFNDNGENAMDLAQRFFKDNIIQFIQGTDFEGDNRSDQ--ETLAFPGHAAAYKGDLEMLKKLVENGIININERDDSESTLLHKAAGQGHVECLQWLIEKGADVNITNKAGELAVD 321 HSP 2 Score: 62.003 bits (149), Expect = 2.945e-6 Identity = 49/167 (29.34%), Postives = 77/167 (46.11%), Query Frame = 1
Query: 469 QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
++TPL +AA G + + L+ HG G T H+AA +G ++ + G LT D+ G + LA T ++ + DP + D P H A+ +G L +Q LL K A+ DTD G +H A + GH+ K+LLS+ +S+
Sbjct: 45 KFTPLHWAAHSGSLECLHWLLWHGADIMSVSPGGWTAAHIAAIKGQDACMQALIMNGAD-----LTLPDDRGCSPSHLAATHGHSYTLQVMLRSGVDPNVSDKCD--WKPVHYASFHGRLGCLQ-LLVKWGAAIEDTDQNGNLPVHLAAMEGHLYCFKFLLSRMSSV 203
BLAST of Alpha-latrocrustotoxin-Lt1a vs. TrEMBL
Match: A0A2B4RXB3 (Ankyrin repeat domain-containing protein 42 OS=Stylophora pistillata OX=50429 GN=Ankrd42 PE=4 SV=1) HSP 1 Score: 194.126 bits (492), Expect = 1.386e-49 Identity = 104/286 (36.36%), Postives = 163/286 (56.99%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+ LHWA+ GS+ +++L+ D + WT +H+A I G+D+CL++L + +N D+RGCT HLAAAHG +C+ + + D NM WTP+ AA+HGR+ ++LL R G + D D G TP HLAA+EGHLP +K+ + G I + A ++ G T LA+ K + + Y+ E D E ++L +P H+AA NGDL ++ L+E G ++VN+ D++G T HKA +GH+ +++WL+ GA++ I ET D+
Sbjct: 17 FTPLHWASHHGSLECLHWLL--WHGADHSDKTPQGWTPSHLAAIRGQDSCLQALAANGAD-LNGKDNRGCTAAHLAAAHGNSYCLQSILRHGVDINATD----NMGWTPVHCAAFHGRLGCLQLLSRWGASVDEVDHSGSTPAHLAASEGHLPCLKFIVCAGA-SIDHTMNARNDQGDTPKNLAQQFYKKDCIDYLNAVEYDLLHPEDEENLAFPAHVAAYNGDLAQLKMLIETGVVSVNERDDKGSTPAHKAAGNGHVQVLQWLVENGANLRIVNSAGETPKDVA 294 HSP 2 Score: 67.781 bits (164), Expect = 6.664e-8 Identity = 51/169 (30.18%), Postives = 77/169 (45.56%), Query Frame = 1
Query: 466 MQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQY-PGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
++TPL +A+ HG + + L+ HG G TP HLAA G ++ G L DN G TA LA ++ I D D++ + P H AA +G L +Q LL + +V++ D+ G T H A GH+ +K+++ GASI
Sbjct: 15 FRFTPLHWASHHGSLECLHWLLWHGADHSDKTPQGWTPSHLAAIRGQDSCLQALAANGAD-----LNGKDNRGCTAAHLAAAHGNSYCLQSILRHGVDINA---TDNMGWTPVHCAAFHGRLGCLQ-LLSRWGASVDEVDHSGSTPAHLAASEGHLPCLKFIVCAGASI 174
BLAST of Alpha-latrocrustotoxin-Lt1a vs. TrEMBL
Match: A0A091IEY5 (Ankyrin repeat domain-containing protein 42 (Fragment) OS=Calypte anna OX=9244 GN=N300_01742 PE=4 SV=1) HSP 1 Score: 188.348 bits (477), Expect = 3.548e-49 Identity = 112/283 (39.58%), Postives = 160/283 (56.54%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
LHWAA GS+ +++L+ +++ WTAAH+A I G+DACL++L +R D RGCT HLAAAHG+ + L N R N++ RN W P+ YAA+HGR+ ++LL+R G D D +G P HLAA EGHL K+ + + IH L A ++ G T LAE KD I++YI EK+ E + L +P H AA GDLL ++ L++ G I +N+ D++G T++HKA GHI ++WL+ GA I ET D+
Sbjct: 43 LHWAAHSGSLECLHWLLWHGADTTEV--TVRGWTAAHLAAIRGQDACLQAL-LRNGANAEAQDDRGCTASHLAAAHGQSYT---LQNILRSGVNVNVSDRN-DWKPVHYAAFHGRLGCLQLLVRWGACVDDVDNNGNLPAHLAAMEGHLHCFKFLVSKMASVIH-TLKARNDQGETPRDLAERFYKDNILQYIDSLEKEREHPESQEVLAFPAHSAAFKGDLLVLRSLVQSGVININERDDKGSTLMHKAAGQGHIHCLQWLIEMGADCDITNDAGETPKDVA 317 HSP 2 Score: 67.3958 bits (163), Expect = 5.579e-8 Identity = 57/177 (32.20%), Postives = 81/177 (45.76%), Query Frame = 1
Query: 469 QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKE-----KDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTL 984
++TPL +AA G + + L+ HG T V G T HLAA G ++ L G + A D+ G TA LA + ++ I D W+ P H AA +G L +Q L+ G+ V+D DN G H A + GH+ K+L+SK AS+ I TL
Sbjct: 39 KFTPLHWAAHSGSLECLHWLLWHGADTTEVTVRGWTAAHLAAIRGQDACLQALLRNGAN-----AEAQDDRGCTASHLAAAHGQSYTLQNILRSGVNVNVSDRNDWK-------PVHYAAFHGRLGCLQLLVRWGAC-VDDVDNNGNLPAHLAAMEGHLHCFKFLVSKMASV-IHTL 201
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Cavefish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2]) HSP 1 Score: 96.6709 bits (239), Expect = 3.170e-20 Identity = 86/282 (30.50%), Postives = 129/282 (45.74%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLL----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEK--DPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
L+ LH A G +V L+D+ L + H+A G C++ L+ + V + + T LH+AA HC HY +++ N +A+ N +TPL A RI ++ELL++HG + G TP+H+AA GH +V + G + G TAL +A + E+VKY+ D ++ D Q P HI++ G IQ LL+ G+ + T GYT LH A GH D+ LL +GAS+ I T
Sbjct: 249 LTPLHCGARSGHEQVVEMLLDRGA--PILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDY-LTALHVAA-----HCGHY--KVAKVIVDKKANPNAKALN-GFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHGASP-----NTTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKS----KDDQTPLHISSRLGKPEIIQQLLQHGACP-DSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITT 509 HSP 2 Score: 83.9593 bits (206), Expect = 3.087e-16 Identity = 73/234 (31.20%), Postives = 103/234 (44.02%), Query Frame = 1
Query: 262 TAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
T HVA G + + L M N + RG T LH+AA G+ + YL + DA+ ++ Q TPL ++ G+ I++ L++HG D T G TPLHLAA EGH + L++G L GFT L +A K E+ + K P P H+AA + LL++G+ N GYT LH A ++I LL GA
Sbjct: 382 TPIHVAAFMGHENIVTQL-MNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNGAYV---DAKSKDDQ-TPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGA-----SLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASG--KSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKN-GYTPLHIAAKKNQMEIATTLLEYGA 602 HSP 3 Score: 71.633 bits (174), Expect = 2.111e-12 Identity = 58/190 (30.53%), Postives = 89/190 (46.84%), Query Frame = 1
Query: 442 NLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
N D + ++ +TPL AA +G I++ LL+ G A D + TPLH+A+ G+ +VK LE G + A +G T L +++V+ + D+ P + + L P H+ A GD LN LL + V+D N T LH A GH + K ++ K A+ K L T + I
Sbjct: 174 NADVESKS-GFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGAR-----IDAKTKDGLTPLHCGARSGHEQVVEMLLDRGA-PILSKTKNGLS-PLHM-ATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIA 354 HSP 4 Score: 70.8626 bits (172), Expect = 3.075e-12 Identity = 59/216 (27.31%), Postives = 104/216 (48.15%), Query Frame = 1
Query: 337 INRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVNDTDNR-GYTMLHKACISGHIDIVKWLLSKGASIWIK 978
IN + G LHLA+ G + + ++ +DA + T L A+ G+ +V+ L+ +G + +G TPL++AA E HL +V+Y L+ G + +GFT L +A + D++V + + + + ++ P HIAA D LL+ A D +++ G+T LH A G+I++ LL++GA++ K
Sbjct: 14 INICNQNGLNALHLASKEGH---VEVVTQLIKMGATVDAATKKGN-TALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQ-----SIATEDGFTPLAVALQQGHDQVVSLLLENDTKGK-------VRLPALHIAARKDDTKAAALLLQNDHNA--DVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFK 211 HSP 5 Score: 66.6254 bits (161), Expect = 6.604e-11 Identity = 64/218 (29.36%), Postives = 96/218 (44.04%), Query Frame = 1
Query: 358 GCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
G T LH+AA +G + L N + D + RN TPL A+ G ++V+LL+ G D DG TPLH A GH +V+ L+ G + K NG + L +A V+ + E ++ +D H+AA G + +++K + N G+T LH AC I +++ LL GASI T T I +
Sbjct: 182 GFTPLHIAAHYGNINVATLLLNRGAAV---DFKARN-DITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKT-----KNGLSPLHMATQGDHLNCVQLLLHHEVP--VDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANP-NAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVA 387 HSP 6 Score: 51.2174 bits (121), Expect = 3.524e-6 Identity = 44/165 (26.67%), Postives = 72/165 (43.64%), Query Frame = 1
Query: 148 TDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQ---CRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLE 633
+ L+ LH AA + + L+D+ P N +T H+A + +L+ + N +G + LHLA+ G + LL DA C TPL AA ++++ E+L+ HG D G TPLH+A G++ +V + L+
Sbjct: 544 SGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNG--YTPLHIAAKKNQMEIATTLLEYGADT-NAITRQGISPLHLASQEGN-------VDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLK 698
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Cavefish
Match: ankrd44 (ankyrin repeat domain 44 [Source:ZFIN;Acc:ZDB-GENE-070912-605]) HSP 1 Score: 90.1225 bits (222), Expect = 2.816e-18 Identity = 89/284 (31.34%), Postives = 136/284 (47.89%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDN-NAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAA--HGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGC----HDIHKC----LTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG----HIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
LH AA G + +V L++ ++D NA TA HVA G+DA + LI V ++P+++G T LH AAA HG C+ +L N +++ Q R+ + +PL A HGR + + LI++G D D DG TPLH+AA GH L+ + G +H L A++ + KL + + I+ + + +++ D G H AA G++ ++ LL GS N TD G T LH A S H ++ L+S G I
Sbjct: 210 LHAAASNGQINVVKHLLN---LSVEIDEANAFGNTALHVACFNGQDAVVSELIDYGANV-SQPNNKGFTPLHFAAASTHGAL-CLEFLVNNG---ADVNVQSRDGK-SPLHMTAVHGRFTRSQTLIQNGGEIDSVDKDGNTPLHIAARYGHELLINTLITSGADCTRRGVHGMFPLHLAALNAHADCCRKLLSSGRRYSIICPLSNDSVLCAGFQI-DTPDSLGRTCLHAAAAGGNVECVKLLLSSGSDH-NRTDKHGRTPLHYAAASRHFQCLETLVSCGTCI 482 HSP 2 Score: 68.1662 bits (165), Expect = 1.958e-11 Identity = 82/304 (26.97%), Postives = 125/304 (41.12%), Query Frame = 1
Query: 76 DDARFKRAVKDIDPVEIRKIIMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQW-TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKE-----KDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
D +A+ + DP EIR +I + D++ L +AE T H A G +E LI+ V N D+ T LH A A + L + D R+ W TPL AA + + E++I + +V+D GRT LH AA GH +V L +G + + A D AL A ++V + D+ KD R + P H AA NG + ++ LL S+ +++ + G T LH AC +G +V L+ GA++
Sbjct: 8 DQPPLLQAIFNGDPEEIRMLIFKSEDINAL---------------------------DAEKRTPLHAAAFLGDAEIIELLILSGARV-NAKDNMWLTPLHRAVASRSEEAVRVLIRHSA-----DVNARDKNWQTPLHVAAANKALRCAEVIIPLLSSVNVSDRGGRTALHHAALNGHTEMVSLLLSKGAN-----INAFDKKDGRALHWAAYMGHLDVVCLLVDQGAEVSCKDKRGYT-------PLHAAASNGQINVVKHLLNL-SVEIDEANAFGNTALHVACFNGQDAVVSELIDYGANV 265 HSP 3 Score: 68.1662 bits (165), Expect = 2.152e-11 Identity = 83/277 (29.96%), Postives = 121/277 (43.68%), Query Frame = 1
Query: 157 SLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDV---TDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG-HIAAVNGDLLNIQGLLEKGSIAVNDT------DNRGYTMLHKACISGHIDIVKWLLSK 957
S +H+AA G + L+++ E D ++ + H+A G LE L+ EV ++ D G T L LAA G C L N + D+ TP+ A +G S V LL+ D+ D G+TPL LA GH+ V LE + D+ G TAL L ++E V+ + ++E + L D H+AA G + LL SIA ++ DN+GYT LH AC +GH V+ LL +
Sbjct: 547 SAVHYAAAYGHRHCLELLLEREENHQDEMESSSTRSPLHLAAYHGHAQALEVLLQGHCEV-DQGDEVGRTALALAALRGHTDCALTLLNHGASPRSRDS---GRGRTPIHLAVMNGHTSCVRLLLEDSDCADLVEAADSQGQTPLMLAVVGGHVDAVSLLLER-----EASVDTADHQGLTALHLGLLCGQEECVQCLLEQEA---SVLLGDSKGRTALHLAAARGHASWLSELL---SIACSEPPTPPLRDNQGYTPLHWACYNGHEGCVEVLLEQ 808 HSP 4 Score: 67.3958 bits (163), Expect = 3.956e-11 Identity = 74/288 (25.69%), Postives = 120/288 (41.67%), Query Frame = 1
Query: 130 KIIMEDTDL------------SLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSK 957
++++ED+D + L A + G V V+ L+++ D+ TA H+ ++ G++ C++ L+ ++ V+ DS+G T LHLAAA G + L + P N +TPL +A ++G VE+L+ D + TPLH A H LE +I C + D A A +++ D D L +AA G + ++ LL S ++ TD +G T LH AC G D +L K
Sbjct: 664 RLLLEDSDCADLVEAADSQGQTPLMLAVVGGHVDAVSLLLEREASVDTADHQG--LTALHLGLLCGQEECVQCLLEQEASVL-LGDSKGRTALHLAAARGHASWLSELLSIACSEPPTPPLRDNQGYTPLHWACYNGHEGCVEVLLEQ-KGCRCFDGNPFTPLHCAVVNDHESCATLLLEAMGSEIVTCKDSRDRAPLHAAAFAGNVDCVQLL-LAHDAPVDAVDQSGRSALM----MAAERGAVGVVEALLTSASADLSLTDQKGNTALHLACSKGKEDCALLILEK 942 HSP 5 Score: 66.2402 bits (160), Expect = 8.060e-11 Identity = 83/320 (25.94%), Postives = 129/320 (40.31%), Query Frame = 1
Query: 157 SLLHWAALKGSVVIVNFLIDKMEFPSDLD---NNAEIWTA--AHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHD----------------IHKCLT-------------AVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKG-----------------SIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
+ LH AAL G +V+ L+ K + D A W A H+ V+ CL L+ + EV + D RG T LH AA++G+ + + +L N + + +A T L A ++G+ ++V LI +G + G TPLH AAA H L FL D +H T +VD +G T L +A + ++ + D + +P H+AA+N + LL G ++ D+ G T LH A G+++ VK LLS G+
Sbjct: 142 TALHHAALNGHTEMVSLLLSKGANINAFDKKDGRALHWAAYMGHLDVV-----CL--LVDQGAEVSCK-DKRGYTPLHAAASNGQINVVKHLLNLSVEIDEANA----FGNTALHVACFNGQDAVVSELIDYGANVSQPNNKGFTPLHFAAASTHGALCLEFLVNNGADVNVQSRDGKSPLHMTAVHGRFTRSQTLIQNGGEIDSVDKDGNTPLHIAARYGHELLINTLITSGADCTRRGVHG--MFPLHLAALNAHADCCRKLLSSGRRYSIICPLSNDSVLCAGFQIDTPDSLGRTCLHAAAAGGNVECVKLLLSSGS 447 HSP 6 Score: 65.4698 bits (158), Expect = 1.227e-10 Identity = 77/291 (26.46%), Postives = 119/291 (40.89%), Query Frame = 1
Query: 121 EIRKIIMEDTDLSLLHWAALKGSVVIVNFL--IDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQ---CRNMQ-WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWI 975
E ++ + + LH AA +G ++ L I E P+ + + +T H A G + C+E L+ +K D T LH A + C T LL + ++ C++ + PL AA+ G + V+LL+ H D D GR+ L +AA G + +V+ L D L+ D G TAL LA +K K++ I +K D + V T++ T LH A SG VK LLS+GAS+ +
Sbjct: 739 EASVLLGDSKGRTALHLAAARGHASWLSELLSIACSEPPTPPLRDNQGYTPLHWACYNGHEGCVEVLLEQKG--CRCFDGNPFTPLHCAVVNDHESCA------TLLLEAMGSEIVTCKDSRDRAPLHAAAFAGNVDCVQLLLAHDAPVDAVDQSGRSALMMAAERGAVGVVEALLTSASAD----LSLTDQKGNTALHLACSKGKEDCALLILEKLPD---------------------------------TTIVKATNSALQTPLHLAAHSGLKQTVKELLSRGASVQV 984 HSP 7 Score: 65.0846 bits (157), Expect = 2.034e-10 Identity = 62/226 (27.43%), Postives = 98/226 (43.36%), Query Frame = 1
Query: 301 CLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDL-QYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWI 975
CLE +++ + D +G + +H AAA+G HC+ R + D + +PL AA+HG +E+L++ D D GRT L LAA GH L G + G T + LA V+ + + E D Q P +A V G + + LLE+ +V+ D++G T LH + G + V+ LL + AS+ +
Sbjct: 527 CLE-FLLKSGATASLKDKQGYSAVHYAAAYGHRHCL--ELLLEREENHQDEMESSSTRSPLHLAAYHGHAQALEVLLQGHCEVDQGDEVGRTALALAALRGHTDCALTLLNHGASPRSR----DSGRGRTPIHLAVMNGHTSCVRLLLEDSDCADLVEAADSQGQTPLMLAVVGGHVDAVSLLLER-EASVDTADHQGLTALHLGLLCGQEECVQCLLEQEASVLL 744 HSP 8 Score: 60.077 bits (144), Expect = 6.935e-9 Identity = 79/274 (28.83%), Postives = 112/274 (40.88%), Query Frame = 1
Query: 181 KGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGC-TLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQW-----TPLMYAAWHGRISIVELLIRHGVATDVTDVD-GRTPLHLAAAEGHLPLVKYFLEEG-CHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG----HIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGAS 966
K + + + FL+ S D ++A H A G CLE L+ R+ + +S + LHLAA HG + L L C Q T L AA G L+ HG + D GRTP+HLA GH V+ LE+ C D+ + A D+ G T L LA + V + ++E D + G H+ + G +Q LLE+ +V D++G T LH A GH + LLS S
Sbjct: 522 KEAALCLEFLLKSGATASLKDKQG--YSAVHYAAAYGHRHCLELLLEREENHQDEMESSSTRSPLHLAAYHGHAQALEVL---------LQGHCEVDQGDEVGRTALALAALRGHTDCALTLLNHGASPRSRDSGRGRTPIHLAVMNGHTSCVRLLLEDSDCADL---VEAADSQGQTPLMLAVVGGHVDAVSLLLEREAS------VDTADHQGLTALHLGLLCGQEECVQCLLEQ-EASVLLGDSKGRTALHLAAARGHASWLSELLSIACS 774 HSP 9 Score: 56.9954 bits (136), Expect = 6.640e-8 Identity = 88/340 (25.88%), Postives = 118/340 (34.71%), Query Frame = 1
Query: 157 SLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTR------LLPNLDAQCRNMQW--------TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDE-----------IVKYIRDKE---------KDPRTWELFDDLQY------------------------------------PGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGAS 966
S LH A+ G LI +D + T H+A G + + +LI + R G LHLAA + C L + R L N C Q T L AA G + V+LL+ G + TD GRTPLH AAA H ++ + G C+ A D G +AL A D V+ ++KE K T L D Y P H+AA +G ++ LL+ G V+ D G T L A + GH D LL+ GAS
Sbjct: 308 SPLHMTAVHGRFTRSQTLIQNGGEIDSVDKDGN--TPLHIAARYGHELLINTLITSGADCTRR-GVHGMFPLHLAALNAHADCCRKLLSSGRRYSIICPLSNDSVLCAGFQIDTPDSLGRTCLHAAAAGGNVECVKLLLSSGSDHNRTDKHGRTPLHYAAASRHFQCLETLVSCG-----TCINATDQWGRSALHYAAASDLDRRRRVALEPESPGVQAEKEKEAALCLEFLLKSGATASLKDKQGYSAVHYAAAYGHRHCLELLLEREENHQDEMESSSTRSPLHLAAYHGHAQALEVLLQ-GHCEVDQGDEVGRTALALAALRGHTDCALTLLNHGAS 638
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Cavefish
Match: ANKRD50 (ankyrin repeat domain 50 [Source:NCBI gene;Acc:103026486]) HSP 1 Score: 88.9669 bits (219), Expect = 9.057e-18 Identity = 83/272 (30.51%), Postives = 126/272 (46.32%), Query Frame = 1
Query: 157 SLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAE---IWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
++L A+ +G+V +V L+D+ LD N WT H+A G ++L+ + + D+ G L LAA G Y C+ L + RN L AA G IVELL+ HG D D DGR L++ A E L + +YFLE G + + D G TAL ++ + E+V+ + D + + ++ + AA G +Q L++ G+ V+ T N+G T L A GHID+V+ LL GA
Sbjct: 810 TVLSIASAQGNVEVVRTLLDR-----GLDENHRDDAGWTPLHMAAFEGHRQVCDALLEQGARC-SEVDNDGRIPLILAAQEGHYDCVQILLENKSCIDQRGYDGRN----SLRVAALEGHRDIVELLLSHGADIDYNDADGRPTLYILALENQLAMAEYFLENGAN-----VETSDTEGRTALHVSCWQGHVEMVRLLISYHADVNSCD--NEKRSALQSAAWQGHSKVVQFLIDSGT-HVDHTCNQGATALGIAAQEGHIDVVRILLEHGA 1063 HSP 2 Score: 80.4925 bits (197), Expect = 3.879e-15 Identity = 82/271 (30.26%), Postives = 125/271 (46.13%), Query Frame = 1
Query: 172 AALKGSVVIVNFLIDKMEFPS--DLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGY---TMLHKACISGHIDIVKWLLSKGASI 969
AA G IV L+D + D+D + AA A + SL++ + ++ D T L +AA G + L + + D R TPL+ AA G S+V L+ G A D D +GRT L +A+A+G++ +V+ L+ G + H+ D+ G+T L +A + ++ + E+ R E+ +D + P +AA G +Q LLE S D RGY L A + GH DIV+ LLS GA I
Sbjct: 678 AAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDCMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGR----TPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHR-----DDAGWTPLHMAAFEGHRQVCDALL--EQGARCSEVDNDGRIPLILAAQEGHYDCVQILLENKSC----IDQRGYDGRNSLRVAALEGHRDIVELLLSHGADI 933 HSP 3 Score: 79.337 bits (194), Expect = 7.320e-15 Identity = 81/265 (30.57%), Postives = 120/265 (45.28%), Query Frame = 1
Query: 193 VIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAA-----AHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQY-PGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
+++N L E + DN TA A G +E L+ EV N D G T L +AA + G + L + + + D C TPL+ AA+ G + +V+LL+ G D TD +GRTPL AA+ GH +V L G + ++D+ G T L +A + E+V+ + D+ D DD + P H+AA G LLE+G+ ++ DN G L A GH D V+ LL + I
Sbjct: 653 IVLNLLQHGAEV-NKADNEGR--TALIAAAYMGHREIVEHLLDHGAEV-NHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDC----MTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGA-----AVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENH---RDDAGWTPLHMAAFEGHRQVCDALLEQGA-RCSEVDNDGRIPLILAAQEGHYDCVQILLENKSCI 900 HSP 4 Score: 68.9366 bits (167), Expect = 1.471e-11 Identity = 67/227 (29.52%), Postives = 97/227 (42.73%), Query Frame = 1
Query: 310 SLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRD-------KEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
+L++ + + D+ G T L LAA G ++ L + + D + WT L AAW G +V L+ G D D D RT L AA GH +V L+ G + DN G TAL A EIV+++ D ++ D RT L P A G + L+++G+ V+ D T L A GH+D+V LL GA +
Sbjct: 556 NLLISRGANMELQDNHGQTPLTLAARQGHTKVVNCLIGCDSNINHTD----HDGWTALRSAAWGGHSEVVSALLYAGAKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAE-----VNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVP----ASKGHASVVSLLIDRGA-EVDHCDKDCMTPLLVAAYEGHVDVVDLLLEGGADV 768 HSP 5 Score: 64.6994 bits (156), Expect = 2.808e-10 Identity = 60/172 (34.88%), Postives = 82/172 (47.67%), Query Frame = 1
Query: 475 TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVK---YIRDK----EKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
T L AA+ G + +V LLI G ++ D G+TPL LAA +GH +V + GC + D++G+TAL+ A E+V Y K + D RT + GH V LN LL+ G+ VN DN G T L A GH +IV+ LL GA +
Sbjct: 541 TLLGNAAYSGNLDVVNLLISRGANMELQDNHGQTPLTLAARQGHTKVVNCLI--GC---DSNINHTDHDGWTALRSAAWGGHSEVVSALLYAGAKVDCADADSRT--ALRAAAWGGHEDIV----LN---LLQHGA-EVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEV 697 HSP 6 Score: 60.8474 bits (146), Expect = 3.765e-9 Identity = 58/197 (29.44%), Postives = 86/197 (43.65%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLD-NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDA---QCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYI 741
L AAL+G IV L + +D+D N+A+ ++ + + A E + V D+ G T LH++ C RLL + A C N + + L AAW G +V+ LI G D T G T L +AA EGH+ +V+ LE G H D G TA+++A I+K +
Sbjct: 911 LRVAALEGHRDIVELL---LSHGADIDYNDADGRPTLYILALENQLAMAEYFLENGANV-ETSDTEGRTALHVS-------CWQGHVEMVRLLISYHADVNSCDNEKRSALQSAAWQGHSKVVQFLIDSGTHVDHTCNQGATALGIAAQEGHIDVVRILLEHGADPNH-----ADQFGRTAMRVAAKGGHSGIIKLL 1091
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Cavefish
Match: psmd10 (proteasome 26S subunit, non-ATPase 10 [Source:NCBI gene;Acc:103037594]) HSP 1 Score: 83.5741 bits (205), Expect = 1.025e-17 Identity = 58/179 (32.40%), Postives = 90/179 (50.28%), Query Frame = 1
Query: 475 TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
T L +A G +IV+ L+ G D+ D TPLH+AA+ G +V+ + +G L +V+ NG T L A +K++ EI + + DP + + P H A+ G+ IQ LL K S + N D+ G T LH AC ++ K L+ GASI+I+ ++T + I
Sbjct: 42 TALHWACSAGHANIVQFLLDVGAEVDLKDDASWTPLHIAASAGREEIVRSLISKGAQ-----LNSVNQNGCTPLHYAASKNQYEIALLLLENGADPNATDKLES--TPLHRASAKGNYRLIQ-LLLKQSASTNIQDSEGNTALHLACDEERVEAAKLLVEHGASIYIENKEEKTPLQIA 212 HSP 2 Score: 81.2629 bits (199), Expect = 5.314e-17 Identity = 58/183 (31.69%), Postives = 93/183 (50.82%), Query Frame = 1
Query: 121 EIRKIIMED------TDL---SLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYH-CMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEG 639
E++K ++ D TD + LHWA G IV FL+D + DL ++A WT H+A G++ + SLI + + +N + GCT LH AA+ +Y + L N PN + ++ TPL A+ G +++LL++ +T++ D +G T LHLA E + K +E G
Sbjct: 21 ELKKCVLSDRAQASRTDQDHRTALHWACSAGHANIVQFLLD-VGAEVDLKDDAS-WTPLHIAASAGREEIVRSLISKGAQ-LNSVNQNGCTPLHYAASKNQYEIALLLLENGAD--PNATDK---LESTPLHRASAKGNYRLIQLLLKQSASTNIQDSEGNTALHLACDEERVEAAKLLVEHG 195
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Cavefish
Match: ripk4 (receptor interacting serine/threonine kinase 4 [Source:NCBI gene;Acc:103022568]) HSP 1 Score: 88.1965 bits (217), Expect = 1.081e-17 Identity = 71/235 (30.21%), Postives = 114/235 (48.51%), Query Frame = 1
Query: 259 WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRH-GVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSK 957
+T H A G +A L L++ + +N D++G T +H+A HGR + + L L DA R +WT L AAW G + IV+LL++ GV D VDGR PLHLA+ G + + ++ G D+ + A + T + ++K+ D + RT + P H+AA G ++ LLE+G+ +DT++ T H A GH +++ LL +
Sbjct: 492 YTPLHFAAQNGDEA-LTRLLLDRAATMNEADAQGRTPIHVACHHGRENVVRVL-----LSRGADAHARGKDEWTALHLAAWQGHLGIVKLLVKQAGVNVDGQTVDGRAPLHLASQRGQYRVARVLVDLGA-DVSITSACLSTPLHVAAETGHTSTSRLLLKHGADIQS--RTAQGL----TPLHLAAWKGHQATVRMLLEEGANP-HDTEHLLRTPCHLAAEGGHCQVMEELLQR 712 HSP 2 Score: 85.8853 bits (211), Expect = 5.196e-17 Identity = 87/295 (29.49%), Postives = 118/295 (40.00%), Query Frame = 1
Query: 262 TAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAA----------------------AHGR----YHCMHYLANFTRLL--PNLDAQCRNM-QWTPLMYAAWHGRISIVELLIRH-GVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDI---HKCLT--------------------------AVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
T H+AV + E L+ RK +N D T LH AA A GR C H N R+L DA R +WT L AAW G + IV+LL++ GV D VDGR PLHLA+ G + + ++ G D+ CL+ + G T L LA K V+ + ++ +P E L+ P H+AA G ++ LL++ AV D G T LH A G+ D + LLS GA I
Sbjct: 459 TPLHLAVEKRLKSVAEILLGRKSTNVNAKDEDQYTPLHFAAQNGDEALTRLLLDRAATMNEADAQGRTPIHVACHHGRENVVRVLLSRGADAHARGKDEWTALHLAAWQGHLGIVKLLVKQAGVNVDGQTVDGRAPLHLASQRGQYRVARVLVDLGA-DVSITSACLSTPLHVAAETGHTSTSRLLLKHGADIQSRTAQGLTPLHLAAWKGHQATVRMLLEEGANPHDTEHL--LRTPCHLAAEGGHCQVMEELLQRWPEAVATPDESGLTPLHIAVRGGYRDAICLLLSHGADI 750 HSP 3 Score: 70.0922 bits (170), Expect = 5.167e-12 Identity = 67/214 (31.31%), Postives = 99/214 (46.26%), Query Frame = 1
Query: 340 NRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
N PDSRG T LHLA L R N++A+ + Q+TPL +AA +G ++ LL+ + D GRTP+H+A G +V+ L G D H A + +TAL LA + IVK + + + D + P H+A+ G + L++ G+ V+ T T LH A +GH + LL GA I +T
Sbjct: 451 NLPDSRGSTPLHLAVEKRLKSVAEIL--LGRKSTNVNAKDED-QYTPLHFAAQNGDEALTRLLLDRAATMNEADAQGRTPIHVACHHGRENVVRVLLSRGA-DAH----ARGKDEWTALHLAAWQGHLGIVKLLVKQAGVNVDGQTVDG-RAPLHLASQRGQYRVARVLVDLGA-DVSITSACLSTPLHVAAETGHTSTSRLLLKHGADIQSRT 654 HSP 4 Score: 52.373 bits (124), Expect = 1.274e-6 Identity = 55/195 (28.21%), Postives = 79/195 (40.51%), Query Frame = 1
Query: 157 SLLHWAALKGSVVIVNFLIDKMEFPSD---LDNNAEI--------WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQW-TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLA 705
+ LH AA +G + IV L+ + D +D A + + A V V G D + S + P LH+AA G H + L D Q R Q TPL AAW G + V +L+ G T+ RTP HLAA GH +++ L+ + + D +G T L +A
Sbjct: 559 TALHLAAWQGHLGIVKLLVKQAGVNVDGQTVDGRAPLHLASQRGQYRVARVLVDLGADVSITSACLSTP-------------LHVAAETG-----HTSTSRLLLKHGADIQSRTAQGLTPLHLAAWKGHQATVRMLLEEGANPHDTEHLLRTPCHLAAEGGHCQVMEELLQR----WPEAVATPDESGLTPLHIA 731
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 92.8189 bits (229), Expect = 1.192e-19 Identity = 84/279 (30.11%), Postives = 127/279 (45.52%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQ-WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYI--RDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
L+ LH AA G +V L+++ L + H+A G C++ L+ + V + TL +L A H HC HY L + R + +TPL A RI ++ELL+++G + + G TP+H++A G+L +V L+ G A + G TAL +A + E+V+ + + D + E Q P HIAA G L +Q LL+ + T N GYT LH A GH D+ LL GAS+ + T
Sbjct: 7 LTPLHCAARSGHEQVVEILLEQGA--PILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDD------ITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASP-----NATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKARE----EQTPLHIAARLGKLEIVQLLLQHMASPDAATSN-GYTPLHVAAREGHEDVTAILLEHGASLAVTT 267 HSP 2 Score: 80.1073 bits (196), Expect = 9.935e-16 Identity = 67/208 (32.21%), Postives = 97/208 (46.63%), Query Frame = 1
Query: 340 NRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
N + RG T LH+AA G+ + L R +DA+ R Q TPL AA G++ IV+LL++H + D +G TPLH+AA EGH + LE G L GFT L +A K E+ + + P + P H+A+ + LL++G+ + + T GYT LH A ++I LL GA
Sbjct: 165 NATNVRGETALHMAARAGQTEVVRSL---LRNGAQVDAKAREEQ-TPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGA-----SLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAG--KNGLTPLHVASHYDNQKVALLLLDQGA-SPHATAKNGYTPLHIAAKKDQLEIATTLLEYGA 360 HSP 3 Score: 67.0106 bits (162), Expect = 1.020e-11 Identity = 81/314 (25.80%), Postives = 121/314 (38.54%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYL---------ANFTRLLP---------------------NLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVN-GDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
LH AA G + IV L+ M P +N +T HVA G + + ++++ + +G T LH+A+ +G+ L A L P + A +N +TPL AA ++ I L+ +G T+ G PLHL A EGH + L H +G + L LA + K + + + K L Y I A + G++ + LL+ G+ VN GYT LH+A GH IV LL GA T+ T + I
Sbjct: 208 LHIAARLGKLEIVQLLLQHMASPDAATSNG--YTPLHVAAREGHED-VTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKN-GYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNH-----ATKSGLSPLHLAAQEDKVNVAEILV---KHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGA-DVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAIA 508 HSP 4 Score: 52.7582 bits (125), Expect = 2.246e-7 Identity = 46/137 (33.58%), Postives = 63/137 (45.99%), Query Frame = 1
Query: 565 DGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEK--DPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
DG TPLH AA GH +V+ LE+G + K NG + L +A E V+ + D T + L H+AA G + LL+KG+ N G+T LH AC I +++ LL GASI
Sbjct: 5 DGLTPLHCAARSGHEQVVEILLEQGAPILAK-----TKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTAL----HVAAHCGHYRTAKVLLDKGANP-NVRALNGFTPLHIACKKNRIRVMELLLKYGASI 131
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 81.6481 bits (200), Expect = 2.337e-16 Identity = 84/293 (28.67%), Postives = 137/293 (46.76%), Query Frame = 1
Query: 136 IMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIW-TAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+ + + LH AA G++ + L++ + +D A T HVA G + ++ L+ R + I+ G T LH AA G + L R P L A+ +N +PL A ++ V+LL+ D +D TPLH+AA GH + K L+ G + L NGFT L +A K++ ++++ + T + P H+A+ G L +Q LL+ G+ + N T+ RG T LH A +G +++VK LL GA + +K +T + I
Sbjct: 200 VTSKSGFTPLHIAAHYGNMNVATLLLN---LGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAK-IDTKTRDGLTPLHCAARSGHEKVIEML--LERDAPIL-AKTKN-GLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARAL-----NGFTPLHIACKKNRIKVMELLLKHGASIHTTT--ESGLTPIHVASFMGHLNVVQLLLQNGA-SSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIA 476 HSP 2 Score: 80.1073 bits (196), Expect = 7.697e-16 Identity = 82/284 (28.87%), Postives = 132/284 (46.48%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSR---GCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAA--VNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQ 990
LS LH A + V L+ + + P D D + T HVA G + L+ R E P++R G T LH+A R M L + TP+ A++ G +++V+LL+++G +++ T+V G TPLH+A+ G L +VK L+ G + A ++ T L +A K EIV+ + + DP P + A+ +GD+ ++ LLE G+ ++ +G+T LH A G +++ LL K AS K GQ
Sbjct: 305 LSPLHMATQGEHLACVQLLLGR-QAPVD-DVTLDYLTPLHVAAHCGHYRVAKLLLDRGGE----PNARALNGFTPLHIACKKNRIKVMELLLKHGASIHT----TTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGA---RVGVKAREDQ--TPLHIASRLGKAEIVQLLLQRGSDPNAPTASGS--TPLYFASREGHGDVASL--LLENGA-DISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQ 568 HSP 3 Score: 72.4034 bits (176), Expect = 2.021e-13 Identity = 72/247 (29.15%), Postives = 108/247 (43.72%), Query Frame = 1
Query: 337 INRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLL---PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVK----------------YIRDKEKDPRTWELFDDLQY-------------PGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
IN + G LHLA+ G + T LL +D + + T L AA G+ IV +L+ G + +G TPL++AA E HL +VK+ L+ G + + +GFT L +A + +++V +I ++ D L LQ+ P HIAA G+ +N+ LL AV+ T G T LH A G+ ++VK LL +GA I KT
Sbjct: 37 INTCNQNGLNALHLASKEGHVDVV------TELLKREAEIDTATK-VGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQ-----SVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALL--LQHEHNADVTSKSGFTPLHIAAHYGN-MNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKT 268 HSP 4 Score: 70.8626 bits (172), Expect = 6.349e-13 Identity = 55/181 (30.39%), Postives = 83/181 (45.86%), Query Frame = 1
Query: 472 WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQY-PGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
+TPL AA ++ + ++L+ G T++ G PLHLAA EGH +V L H ++ G TA+ LA + K + + + + D T L Y P H+A+ G++ + LL ++ VN GYT LH+A GH +V LL AS T T + I
Sbjct: 604 YTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGR-----HASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTT---KLCYTPLHVASHYGNIKMVNFLLRNRAL-VNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTALAIA 775 HSP 5 Score: 70.0922 bits (170), Expect = 9.352e-13 Identity = 79/284 (27.82%), Postives = 115/284 (40.49%), Query Frame = 1
Query: 259 WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDK--EKDPRTWE---------------------LFDDLQYPG----------HIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
T HVA G ++ L+++ N + RG T LH+A+ G+ + L + + R Q TPL A+ G+ IV+LL++ G + G TPL+ A+ EGH + LE G DI + V GFT L +A + E+ + +K DP+ L D P HIAA L + LLE G+ N +G LH A GH D+V LL + ASI + T G T + +
Sbjct: 404 LTPIHVASFMGHLNVVQ-LLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSG---ARVGVKAREDQ-TPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGA-DI----SPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGA-ETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLA 676 HSP 6 Score: 48.1358 bits (113), Expect = 6.426e-6 Identity = 43/155 (27.74%), Postives = 69/155 (44.52%), Query Frame = 1
Query: 310 SLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKC-LTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTW 771
+L++ + I+ G T +HLAA + + L + C +TPL A+ +G I +V L+R+ + +G TPLH AA +GH +V L+ H+ LTA NG TAL +A +V + ++P T
Sbjct: 653 ALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLC----YTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQ---HEASPNELTA---NGNTALAIARRLGYISVVDTLSILTEEPVTG 797 HSP 7 Score: 47.7506 bits (112), Expect = 8.026e-6 Identity = 42/139 (30.22%), Postives = 67/139 (48.20%), Query Frame = 1
Query: 565 DGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
D T AA G+L + L+ G DI+ C + NG AL LA + ++V + +E + T + HIAA+ G ++ L+E G+ +N G+T L+ A H++IVK+LL GA+ + T
Sbjct: 10 DAATSFLRAARNGNLERLMDHLKGGM-DINTC----NQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTAL--HIAALAGQTDIVRVLVEFGA-NINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVAT 140
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 81.6481 bits (200), Expect = 2.477e-16 Identity = 84/290 (28.97%), Postives = 137/290 (47.24%), Query Frame = 1
Query: 145 DTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIW-TAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
++ + LH AA G++ + L++ + +D A T HVA G + ++ L+ R + I+ G T LH AA G + L R P L A+ +N +PL A ++ V+LL+ D +D TPLH+AA GH + K L+ G + L NGFT L +A K++ ++++ + T + P H+A+ G L +Q LL+ G+ + N T+ RG T LH A +G +++VK LL GA + +K +T + I
Sbjct: 210 ESGFTPLHIAAHYGNMNVATLLLN---LGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAK-IDTKTRDGLTPLHCAARSGHEKVIEML--LERDAPIL-AKTKN-GLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARAL-----NGFTPLHIACKKNRIKVMELLLKHGASIHTTT--ESGLTPIHVASFMGHLNVVQLLLQNGA-SSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIA 483 HSP 2 Score: 77.0258 bits (188), Expect = 7.338e-15 Identity = 79/276 (28.62%), Postives = 129/276 (46.74%), Query Frame = 1
Query: 154 LSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSR---GCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAA--VNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGAS 966
LS LH A + V L+ + + P D D + T HVA G + L+ R E P++R G T LH+A R M L + TP+ A++ G +++V+LL+++G +++ T+V G TPLH+A+ G L +VK L+ G + A ++ T L +A K EIV+ + + DP P + A+ +GD+ ++ LLE G+ ++ +G+T LH A G +++ LL K AS
Sbjct: 312 LSPLHMATQGEHLACVQLLLGR-QAPVD-DVTLDYLTPLHVAAHCGHYRVAKLLLDRGGE----PNARALNGFTPLHIACKKNRIKVMELLLKHGASIHT----TTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGA---RVGVKAREDQ--TPLHIASRLGKAEIVQLLLQRGSDPNAPTASGS--TPLYFASREGHGDVASL--LLENGA-DISPVTKKGFTPLHIASKYGRLEVATLLLEKYAS 567 HSP 3 Score: 72.4034 bits (176), Expect = 2.125e-13 Identity = 78/282 (27.66%), Postives = 114/282 (40.43%), Query Frame = 1
Query: 259 WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDK--EKDPRTWELFDDLQY-----------------------------PGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
T HVA G ++ L+++ N + RG T LH+A+ G+ + L + + R Q TPL A+ G+ IV+LL++ G + G TPL+ A+ EGH + LE G DI + V GFT L +A + E+ + +K DP+ L P HIAA L + LLE G+ N +G LH A GH D+V LL + ASI + T G T + +
Sbjct: 411 LTPIHVASFMGHLNVVQ-LLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSG---ARVGVKAREDQ-TPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGA-DI----SPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGA-ETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLA 681 HSP 4 Score: 70.8626 bits (172), Expect = 5.110e-13 Identity = 81/320 (25.31%), Postives = 129/320 (40.31%), Query Frame = 1
Query: 151 DLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDAC--LESLIMRKPEVINRPDSRGCTLLHLAAAHGR-------------------------YHCMHYLANFTRLL-----PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQY-PGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
D + LH A+ G IV L+ + P NA + + ++ + SL++ I+ +G T LH+A+ +GR + HY LL + + +N +TPL AA ++ + ++L+ G T++ G PLHLAA EGH +V L H ++ G TA+ LA + K + + + + D T L Y P H+A+ G++ + LL ++ VN GYT LH+A GH +V LL AS T T + I
Sbjct: 476 DQTPLHIASRLGKAEIVQLLLQRGSDP-----NAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKN-GYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGR-----HASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTT---KLCYTPLHVASHYGNIKMVNFLLRNRAL-VNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTALAIA 780 HSP 5 Score: 69.3218 bits (168), Expect = 1.617e-12 Identity = 72/255 (28.24%), Postives = 108/255 (42.35%), Query Frame = 1
Query: 337 INRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLL---PNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVK----------------YIRDKEKDPRTWELFDDLQY---------------------PGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
IN + G LHLA+ G + T LL +D + + T L AA G+ IV +L+ G + +G TPL++AA E HL +VK+ L+ G + + +GFT L +A + +++V +I ++ D L LQ+ P HIAA G+ +N+ LL AV+ T G T LH A G+ ++VK LL +GA I KT
Sbjct: 36 INTCNQNGLNALHLASKEGHVDVV------TELLKREAEIDTATK-VGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQ-----SVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALL--LQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGN-MNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKT 275 HSP 6 Score: 63.5438 bits (153), Expect = 1.225e-10 Identity = 54/186 (29.03%), Postives = 82/186 (44.09%), Query Frame = 1
Query: 454 QCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
Q +TPL AA +G +++ LL+ G A D T +G TPLH+AA G+ +VK L+ G K +G T L A +++++ + E+D + P H+A L +Q LL + V+D T LH A GH + K LL +G + L T + I
Sbjct: 207 QTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKT-----RDGLTPLHCAARSGHEKVIEMLL--ERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGR-QAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIA 384 HSP 7 Score: 48.1358 bits (113), Expect = 5.606e-6 Identity = 43/155 (27.74%), Postives = 69/155 (44.52%), Query Frame = 1
Query: 310 SLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKC-LTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTW 771
+L++ + I+ G T +HLAA + + L + C +TPL A+ +G I +V L+R+ + +G TPLH AA +GH +V L+ H+ LTA NG TAL +A +V + ++P T
Sbjct: 658 ALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLC----YTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQ---HEASPNELTA---NGNTALAIARRLGYISVVDTLSILTEEPVTG 802 HSP 8 Score: 47.7506 bits (112), Expect = 8.450e-6 Identity = 42/139 (30.22%), Postives = 67/139 (48.20%), Query Frame = 1
Query: 565 DGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
D T AA G+L + L+ G DI+ C + NG AL LA + ++V + +E + T + HIAA+ G ++ L+E G+ +N G+T L+ A H++IVK+LL GA+ + T
Sbjct: 9 DAATSFLRAARNGNLERLMDHLKGGM-DINTC----NQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTAL--HIAALAGQTDIVRVLVEFGA-NINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVAT 139
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007449.1 (pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007449.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 79.337 bits (194), Expect = 1.403e-15 Identity = 67/208 (32.21%), Postives = 97/208 (46.63%), Query Frame = 1
Query: 340 NRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
N + RG T LH+AA G+ + L R +DA+ R Q TPL AA G++ IV+LL++H + D +G TPLH+AA EGH + LE G L GFT L +A K E+ + + P + P H+A+ + LL++G+ + + T GYT LH A ++I LL GA
Sbjct: 75 NATNVRGETALHMAARAGQTEVVRSL---LRNGAQVDAKAREEQ-TPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHG-----ASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAG--KNGLTPLHVASHYDNQKVALLLLDQGA-SPHATAKNGYTPLHIAAKKDQLEIATTLLEYGA 270 HSP 2 Score: 77.0258 bits (188), Expect = 7.380e-15 Identity = 58/172 (33.72%), Postives = 86/172 (50.00%), Query Frame = 1
Query: 472 WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYI--RDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKT 981
+TPL A RI ++ELL+++G + + G TP+H++A G+L +V L+ G A + G TAL +A + E+V+ + + D + E Q P HIAA G L +Q LL+ + T N GYT LH A GH D+ LL GAS+ + T
Sbjct: 16 FTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASP-----NATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKARE----EQTPLHIAARLGKLEIVQLLLQHMASPDAATSN-GYTPLHVAAREGHEDVTAILLEHGASLAVTT 177 HSP 3 Score: 65.855 bits (159), Expect = 2.142e-11 Identity = 81/314 (25.80%), Postives = 121/314 (38.54%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYL---------ANFTRLLP---------------------NLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVN-GDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
LH AA G + IV L+ M P +N +T HVA G + + ++++ + +G T LH+A+ +G+ L A L P + A +N +TPL AA ++ I L+ +G T+ G PLHL A EGH + L H +G + L LA + K + + + K L Y I A + G++ + LL+ G+ VN GYT LH+A GH IV LL GA T+ T + I
Sbjct: 118 LHIAARLGKLEIVQLLLQHMASPDAATSNG--YTPLHVAAREGHED-VTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKN-GYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNH-----ATKSGLSPLHLAAQEDKVNVAEIL---VKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGA-DVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAIA 418
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003991.1 (pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1 gene:ENSPMAG00000003623.1 transcript:ENSPMAT00000003991.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 79.337 bits (194), Expect = 1.541e-15 Identity = 86/283 (30.39%), Postives = 129/283 (45.58%), Query Frame = 1
Query: 157 SLLHWAALKGSVVIVNFLIDKMEFPSDLDN-NAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAA--HGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGC----HDIHKC----LTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPG---HIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
S LH A+ G + +V L+D D+D+ N+ TA HVA G+D + LI V N+ + RG T LH AAA HG C+ L N +++ Q ++ + +PL A HGR + + LI++G D D DG TPLH+AA GH L+ + G IH L A+ KL K IV ++ +E+ ++ H AA G++ + LL G+ +N D T LH A + ++ V L++ GA
Sbjct: 206 SPLHAASSSGQIGVVKLLLD---LGVDIDDPNSFGNTALHVACYNGQDVVVNELIDCGANV-NQANERGFTPLHFAAASTHGAL-CLELLVNNGA---DVNVQSKDGK-SPLHMTAVHGRFTRSQTLIQNGGEIDCEDRDGNTPLHIAARYGHELLINTLITNGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSAGQKYSIVSSFSNEHVLSAGFEIDTPDEFGRTCLHAAASGGNVECLNLLLSTGA-DMNKKDRFERTALHYAAANCNVQCVVTLVASGA 478 HSP 2 Score: 78.9518 bits (193), Expect = 2.083e-15 Identity = 70/227 (30.84%), Postives = 105/227 (46.26%), Query Frame = 1
Query: 301 CLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFD-DLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIK 978
CLE L +RK D +G T +H AAA+G C+ L + D + +PL AA+HG +E+LIR V DV + +G TPL LAA GH V+ ++ G L + TAL A T E ++ + + + D + Q P +A NG LL KG+ ++N DN+G T LH+ +GH + V LL + + ++
Sbjct: 530 CLEHL-LRKDADPGIQDKQGYTPVHYAAAYGHRVCLELLLEQPGAFQS-DGK-SGHPISPLHLAAYHGHHGALEVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGA----SVLLKEGPDRRTALHSAATNGHVECLRLLVENADSNEAVDASDANEQTPVMLAVGNGHTDCASLLLSKGA-SINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLR 748 HSP 3 Score: 68.1662 bits (165), Expect = 4.222e-12 Identity = 72/303 (23.76%), Postives = 124/303 (40.92%), Query Frame = 1
Query: 157 SLLHWAALKGSVVIVNFLIDKMEFPSDLD-NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQ----YPGHIAAVNGDLLNIQGLLEK-GSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTL------------GQETVIDIV 1011
+ LH AA G V + L++ + +D ++A T +AV G C SL++ K IN D++GCT LH AA G C V+LL++ V + + GR PLH+AAA GH+ ++ ++ + D NG+T L A D V+ + + ++F + P H A +N + + L++ G+ +N D +G LH A + H++ ++ LLS A + + + GQ T ++++
Sbjct: 652 TALHSAATNGHVECLRLLVENADSNEAVDASDANEQTPVMLAVGNGHTDC-ASLLLSKGASINAKDNKGCTALHRGAATGHEEC-------------------------------------VDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILSALVQAAMASGAP-IPLTDRNGYTPLHWACYNGHDGCVELLLE-------LDVFQKQEGNAFSPLHCAVLNDNESCAEMLIDTFGAKIINCPDAKGRMPLHAAAFTDHVECLQLLLSHNALVNVTDMAGKSPLMMACENGQATAVEVL 908 HSP 4 Score: 60.8474 bits (146), Expect = 8.247e-10 Identity = 74/252 (29.37%), Postives = 115/252 (45.63%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKM---EFPSDL-DNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANF--TRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFD-DLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYT 897
LH AA G + I++ L+ P L D N +T H A G D C+E L+ + +V + + + LH A + C L + +++ DA+ R PL AA+ + ++LL+ H +VTD+ G++PL +A G V+ + D L D N TAL LA ++S + I DK D + LQ P H+AA +G +Q LL KG+ ++ D+ GYT
Sbjct: 756 LHMAAACGHMGILSALVQAAMASGAPIPLTDRNG--YTPLHWACYNGHDGCVELLL--ELDVFQKQEGNAFSPLHCAVLNDNESCAEMLIDTFGAKIINCPDAKGR----MPLHAAAFTDHVECLQLLLSHNALVNVTDMAGKSPLMMACENGQATAVEVLVNSTKTD----LKLQDANRNTALHLACSRSHETCALLILDKLDDLNLINATNAALQTPLHLAARSGMATVVQVLLNKGA-SILAVDHNGYT 994 HSP 5 Score: 60.4622 bits (145), Expect = 1.161e-9 Identity = 84/361 (23.27%), Postives = 143/361 (39.61%), Query Frame = 1
Query: 82 ARFKRAVKDIDPVEIRKII--------MEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIW-TAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHC---------------------MHYLA--NFTRLLPNLDAQCRNMQW------TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEG-----------------CH-----------DIHKCLTAVDNNGFTALKLAETKSKDEI-VKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
A +A+ + DP E+R +I ++D + LH AA G I+ L+ + DN W T H A +A +++L+ +V N D T LH+AAA+ C +H+ A + ++ L A+ N+ + +AA+ G +V++L+ HG D G +PLH A++ G + +VK L+ G C+ D + + GFT L A + + ++ + + D D + P H+ AV+G Q L++ G ++ D G T LH A GH ++ L++ GA
Sbjct: 8 APLIQAIFNGDPDEVRSLIYKKEEVNAVDDEKRTPLHAAAYLGEAEIIELLVLSGARVNSKDNK---WLTPLHRACASRSEAAVQTLLKHSADV-NARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVVNELIDCGANVNQANERGFTPLHFAAASTHGALCLELLVNNGADVNVQS--KDGKSPLHMTAVHGRFTRSQTLIQNGG-EIDCEDRDGNTPLHIAARYGHELLINTLITNGA 361 HSP 6 Score: 55.0694 bits (131), Expect = 4.953e-8 Identity = 66/232 (28.45%), Postives = 99/232 (42.67%), Query Frame = 1
Query: 277 AVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQW-TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
A+ G + SLI +K EV N D T LH AA G + L +L ++ +W TPL A + V+ L++H + D + +TPLH+AAA + + + + D G TAL A E+V ++ K + ++ D H AA G ++ L+ G+ V D +GY+ LH A SG I +VK LL G I
Sbjct: 13 AIFNGDPDEVRSLIYKKEEV-NAVDDEKRTPLHAAAYLGEAEIIELL-----VLSGARVNSKDNKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAANRAVKCAEAIIP-----MLSSSNVSDRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAI--HWAAYMGHTEVVKVLVAHGA-DVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDI 230 HSP 7 Score: 53.9138 bits (128), Expect = 1.096e-7 Identity = 51/195 (26.15%), Postives = 83/195 (42.56%), Query Frame = 1
Query: 475 TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIV----KYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI------------WIKTLGQETVIDIV 1011
PL+ A ++G V LI + D + RTPLH AA G +++ + G + + DN T L A + V K+ D + W Q P H+AA N + + ++ S + N +D G T LH A +GH ++V +L++KGA+I W +G V+ ++
Sbjct: 8 APLIQAIFNGDPDEVRSLIYKKEEVNAVDDEKRTPLHAAAYLGEAEIIELLVLSGAR-----VNSKDNKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNW------QTPLHVAAANRAVKCAEAIIPMLS-SSNVSDRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVL 190 HSP 8 Score: 51.6026 bits (122), Expect = 5.014e-7 Identity = 63/240 (26.25%), Postives = 97/240 (40.42%), Query Frame = 1
Query: 337 INRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLH-LAAAEGHLPLVKYF------------LEEGCHDIHKCLTAV----------DNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNR---GYTMLHKACISGHIDIVKWLLSKGASIWIK 978
I+ PD G T LH AA+ G C++ L + + D + T L YAA + + V L+ G + D TPLH AAA+G V + + C + CL + D G+T + A ++ + ++ ++ P H+AA +G G LE ++ D D R GYT L A + GH D V+ L+ GAS+ +K
Sbjct: 414 IDTPDEFGRTCLHAAASGGNVECLNLLLSTGADMNKKD----RFERTALHYAAANCNVQCVVTLVASGARLNEQDERRCTPLHYAAAADGDGSTVSFSVYNTISVQSYIDVHSTCSEAQICLEHLLRKDADPGIQDKQGYTPVHYAAAYGHRVCLELLLEQPGAFQSDGKSGHPISPLHLAAYHGH----HGALEVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGASVLLK 645 HSP 9 Score: 48.1358 bits (113), Expect = 6.011e-6 Identity = 49/172 (28.49%), Postives = 72/172 (41.86%), Query Frame = 1
Query: 475 TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAET----KSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIK 978
TPL AA+ G I+ELL+ G + D TPLH A A V+ L+ + A D N T L +A K + I+ + R + H AA NG + L+ KG+ +N D + +H A GH ++VK L++ GA + K
Sbjct: 41 TPLHAAAYLGEAEIIELLVLSGARVNSKDNKWLTPLHRACASRSEAAVQTLLKHSAD-----VNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAG------RTALHHAAFNGHSEMVNFLVAKGA-NINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCK 200
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464]) HSP 1 Score: 56.9954 bits (136), Expect = 1.197e-9 Identity = 45/136 (33.09%), Postives = 63/136 (46.32%), Query Frame = 1
Query: 550 DVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSK 957
D D G TP H+A + G+L +VK + + L + N G T L LA K E+ +++ + R + F+ Q P H AA G L I+ L G AVN D +G+T L A GH D L+ K
Sbjct: 66 DYPDDSGWTPFHIACSVGNLEVVKSLYDR---PLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFN--QIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEK 196 HSP 2 Score: 53.5286 bits (127), Expect = 1.707e-8 Identity = 50/157 (31.85%), Postives = 70/157 (44.59%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKME------FPSDLDNNAEIWTAAHVAVIGGKDACLESLIMR--KPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHG-VATDVTDVDGRTPLHLAAAEGH 606
LHW+ + I +FL+ KME +P D WT H+A G ++SL R KP+ +N+ ++G T LHLA + +L + D PL AA G + ++ELL G A + D G TPL A AEGH
Sbjct: 40 LHWSVSFQAHEITSFLLSKMENVNLDDYPDD-----SGWTPFHIACSVGNLEVVKSLYDRPLKPD-LNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKD----KFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGH 186
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Yeast
Match: AKR1 (Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant [Source:SGD;Acc:S000002672]) HSP 1 Score: 58.5362 bits (140), Expect = 2.024e-9 Identity = 46/174 (26.44%), Postives = 80/174 (45.98%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIW--TAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQW-TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVD 675
LHWA++ + +V+FL+ + +D++ A T H A G ++ L+ + D +G LLHL+ + Y+ LD CR+ + T L++AA+ G V L++ G + + D +G TPLH +G ++KY +++G K T D
Sbjct: 113 LHWASINNRLSVVDFLVSQ---GADVNARAGALHATPLHWAARYGYVYIVDFLLKHGADP-TMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYLIQDGADFFQKTDTGKD 282 HSP 2 Score: 49.2914 bits (116), Expect = 1.686e-6 Identity = 51/195 (26.15%), Postives = 80/195 (41.03%), Query Frame = 1
Query: 262 TAAHVAVIGGKDACLESLIMRKP-EVINRPDS-RGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFL----EEGCHDI-----------------------------HKCLTAVDNNGFTALKLAETKSKDEIVKYI 741
T H A G A ++ +I K EV N DS T LH A+ + R + +L + +++A+ + TPL +AA +G + IV+ L++HG +TD G LHL+ ++ LV Y L +G DI + D GFT L K + ++KY+
Sbjct: 75 TRYHTACQRGDLATVKEMIHGKLLEVNNDGDSTEHITGLHWASINNRLSVVDFLVSQGA---DVNARAGALHATPLHWAARYGYVYIVDFLLKHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYL 266
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Yeast
Match: HOS4 (Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate [Source:SGD;Acc:S000001374]) HSP 1 Score: 56.6102 bits (135), Expect = 1.070e-8 Identity = 37/100 (37.00%), Postives = 49/100 (49.00%), Query Frame = 1
Query: 349 DSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVD--GRTPLHLAAAEGHLPLVKYFLEEGC 642
DS G T L +A G+Y + + N N T L AA G I IVELLI +G ++ ++ G TPL A+A GHL +VKY L+ G
Sbjct: 327 DSGGRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGN---TALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGA 423 HSP 2 Score: 53.9138 bits (128), Expect = 7.505e-8 Identity = 27/71 (38.03%), Postives = 42/71 (59.15%), Query Frame = 1
Query: 802 HIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTL---GQETVID 1005
IA G ++ ++E+G +ND DN G T LH+A + GHI+IV+ L+ GA + IK++ G +ID
Sbjct: 335 QIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLID 405
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Yeast
Match: AKR2 (Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication [Source:SGD;Acc:S000005560]) HSP 1 Score: 49.2914 bits (116), Expect = 1.675e-6 Identity = 58/217 (26.73%), Postives = 90/217 (41.47%), Query Frame = 1
Query: 370 LHLAAAHGRYHCMHYL----ANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDI 1008
LH A + R+ +L AN PN A T L +AA +G I IV+LL++HG + D G +H + ++ LV + V+NN + SKD + + P W AA GD L ++ LL+ GS V TDNRG+ LH A + G ++ L+ GA+ + + ++ D+
Sbjct: 88 LHWACINNRFSVAKFLLLRGAN-----PNQAAGPGGA--TALHWAARYGNIYIVDLLLKHGADPTLKDEQGLNIMHFSVYSSNILLVV----------YVLYFVVNNN-----DNVDIDSKD-------NNNRTPLLW------------AAYQGDFLTVELLLKFGS-TVAWTDNRGFNALHCALVGGDQRVICDLILSGANFYERNNQKQDCFDL 262
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Yeast
Match: AVO2 (Component of a complex containing the Tor2p kinase and other proteins; complex may have a role in regulation of cell growth [Source:SGD;Acc:S000004672]) HSP 1 Score: 46.9802 bits (110), Expect = 7.169e-6 Identity = 48/205 (23.41%), Postives = 78/205 (38.05%), Query Frame = 1
Query: 73 DDDARFKRAVKDIDPVEIRKIIMEDTDL----------SLLHWAALKGSVVIVNFLI----DKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATD--VTDVDGRTPLHLAAAEGHLPLVKYFLEEG 639
+ R + A+ + + + +++++ + DL S LH+A+ G +I +LI DK E N T H+A++ G + L L+ + P IN G +H+A + Y C+ LLI GV D V D +G TPLH+ G + +K L EG
Sbjct: 4 EPSVRLREAIIEGNLLIVKRLLRRNPDLLTNIDSENGWSSLHYASYHGRYLICVYLIQLGHDKHELIKTFKGN----TCVHLALMKGHEQTLHLLLQQFPRFINHRGENGRAPIHIACMNDYYQCL-------------------------------------SLLI--GVGADLWVMDTNGDTPLHVCLEYGSISCMKMLLNEG 165
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Nematostella
Match: EDO43381 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYU3]) HSP 1 Score: 74.3294 bits (181), Expect = 9.107e-14 Identity = 54/168 (32.14%), Postives = 83/168 (49.40%), Query Frame = 1
Query: 232 SDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLD-AQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIV 732
S+ +N + H A G A ++ LI P + + PD +G T LH+A+++G C+ +LA + +D Q +T + AA G ++ + +L G + VD +TPLHLAA GHL VK+ L A D+NG TAL +AE ++IV
Sbjct: 893 SESENVFRTGPSIHDAAKKGDLARIKELIGVAPAMKSVPDEKGMTALHIASSNGHLDCVKWLA-----VSGVDLGQETPTGYTAIHLAAMGGHVNCMMILSAMGSVVNCKTVDDQTPLHLAAMSGHLECVKWLLANRSR-----TEAEDSNGRTALDIAEEYGHEDIV 1050
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Nematostella
Match: EDO38725 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC11]) HSP 1 Score: 69.3218 bits (168), Expect = 1.975e-13 Identity = 59/214 (27.57%), Postives = 95/214 (44.39%), Query Frame = 1
Query: 337 INRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGF---TALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
+N+ D G + LH A RY+ + +A R + ++ TPL A G + + LL+ G A D + G TPLH+AA G +K+ L +G A+D F T A + E + +K+++ ++ P H+A G L+++ L G ++ N+ D G + LH A +SG +V LL GA I
Sbjct: 1 LNKMDDTGFSALHHAT---RYNRVEAMAELIR-----NGSIEDLS-TPLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKG--------VAIDERDFSHRTPFLSAVAAGRTENAMLLLEKDENQKS---------PLHLACAAGRALHVEALTRAG-LSTNERDEEGQSALHMAALSGFRKVVTQLLRYGADI 187
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Nematostella
Match: EDO38702 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC57]) HSP 1 Score: 70.8626 bits (172), Expect = 5.488e-13 Identity = 68/202 (33.66%), Postives = 87/202 (43.07%), Query Frame = 1
Query: 175 ALKGSVVIVNFLIDKMEFPSDL--DNNAEI-------WTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDA-QCRNMQ-WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRD 747
L G V N LI + S L DN A I T H A G+ + +L+ R P +IN D T LHLAA HG + A LL A RN WTPL AA G + + +L+ D TD TPLHLA+ EGH+ +VK L + +T D+ G L +A S E+ I D
Sbjct: 247 CLNGLVGGSNKLILVLRISSQLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHG------HAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKAN-----ITLTDSAGRNCLDIAIESSHKEVAVAIID 437 HSP 2 Score: 57.3806 bits (137), Expect = 1.169e-8 Identity = 79/324 (24.38%), Postives = 128/324 (39.51%), Query Frame = 1
Query: 139 MEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSD--------LDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPD-------------SRGCTLLHLAAAHGRYHCMHYLANFTR--LLPNLDA--------------QCRNM-QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEG------CHDIHKCLTAV---DNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
+++ ++ LH+A ++G++ + L E D + E TA H+AV GG L L + K +N +R C + + + +Y R LL D +CR+ + TPLM AA + V L++HG D++ R + AAAEG + ++ LE C D+ CL + N L+++ D D + D +D+ P H AA G + + LL++ +ND D T LH A + GH +VK LL +G +I
Sbjct: 33 LDEEQMTPLHFACMEGNLGVAKLLFVAAELRGGWSTVSKMVTDQDREEQTALHLAVEGGHGD-LAKLCLEKGANVNAVKESKNKTFHKTIVVARECIFVMIKVSKLKYLSFCKSEKIKREILLYFFDVVIIICYIIRFRACIECRDKDKDTPLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICDDV--CLNGLVGGSNKLILVLRISSQLLLDN--GACIDSKND-------EDIT-PIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAI 343 HSP 3 Score: 54.6842 bits (130), Expect = 8.552e-8 Identity = 53/180 (29.44%), Postives = 72/180 (40.00%), Query Frame = 1
Query: 475 TPLMYAAWHGRISIVELLIRHGVATDVTDVDG--RTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDI 1008
TP+ YAA GR+ V L++ A + D DG TPLHLAA GH +VK L G A+D T L P AA G ++ + +L V+ TD T LH A GH+++VK LLS+ A+I + +DI
Sbjct: 285 TPIHYAAKFGRVRTVCALLKRDPAI-INDEDGASNTPLHLAALHGHAAVVKELLYRG--------GAIDARNAT--------------------------------LWTPLDCAAARG-MVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDI 422
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Nematostella
Match: EDO37520 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFL9]) HSP 1 Score: 70.8626 bits (172), Expect = 1.338e-12 Identity = 75/304 (24.67%), Postives = 122/304 (40.13%), Query Frame = 1
Query: 115 PVEIRKIIMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSR--------------------GCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGAS 966
P E+ T+++ LH+AA + ++ L + LD N+ T A +A G+ L L + + + +S G +LLH AA G + +L N +L + ++ N TP+ AA +GR+S++E+L +HG D PL AAA GHLP V++ + G D +C+ ++ + K + K + D+ W L HIA+ + +L A+ D G LHKA + I +K L G S
Sbjct: 791 PSELLNSSDSTTNMTALHFAASTSNTGVIGLLANHFSTVDCLDRNSR--TPAFIAASTGQKNILAILFQKGANLFHVTNSLSKKHQQRAKNPVKDCKRLMCGYSLLHAAAQGGHVETVKFLLNH-KLSKDAESGAGN---TPMQIAAQYGRVSVMEVLHKHGAPCD------PRPLRSAAAGGHLPAVRFLHQAGVSD--ECVPC-------KYSISPSYMKQRVGKLVDDE----YLWRCETAL----HIASAMEQPDIVSYILRSNDTALTCYDYLGMRPLHKAVVYNSIKSLKAFLEYGVS 1065
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Nematostella
Match: EDO36720 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHV7]) HSP 1 Score: 66.2402 bits (160), Expect = 4.213e-12 Identity = 60/200 (30.00%), Postives = 92/200 (46.00%), Query Frame = 1
Query: 475 TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGC-----HDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDK--EKDPRTWELFDDLQYPG---------------HIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDI 1008
T L A + G + +V+LL+ +G D+ D +G + LH AA G+L +++Y + G +D+ + V + + LAE + + K + DK K P+ L D G H A GD ++Q LLE G+ VN G + LH+A SG+ID LL GA ++ +T G IDI
Sbjct: 48 TALQRACFTGNLKLVQLLVSYGANLDIQDKEGWSVLHAAAVAGNLSILRYLVAVGADVSVRNDLGELPIDVATDVHCVIVLAEGMKRAGLRK-LTDKFFAKRPQLKTLIKDSLIQGLDNVNDLNDSGISLLHEMAGRGDSESVQFLLEHGA-EVNRQSLNGSSPLHEAVQSGNIDCTLLLLRSGADLYAETDGGFLPIDI 245
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Medaka
Match: psmd10 (proteasome 26S subunit, non-ATPase 10 [Source:NCBI gene;Acc:101166639]) HSP 1 Score: 88.9669 bits (219), Expect = 1.374e-19 Identity = 57/183 (31.15%), Postives = 94/183 (51.37%), Query Frame = 1
Query: 121 EIRKIIMEDTDLSL---------LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYH-CMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEG 639
++++ I+ D L+ LHWA G IV FL+D + +L ++A WT H+A G++ +++LI + + +N + GCT LH AA+ RY + L N PN + ++WTPL A+ G +++LL++ +T++ D G TPLHLA E + K +E G
Sbjct: 21 KLKQCILSDKTLACKTDQDRRTALHWACSAGHTNIVEFLLD-LGVEVNLQDDAS-WTPLHIAASAGREDIVKALISKGAQ-LNSVNQNGCTPLHYAASKDRYEIALMLLENGAD--PNATDK---LEWTPLHRASAKGNYRLIQLLLKQSASTNIQDCQGNTPLHLACEEERVEAAKLLVEHG 195 HSP 2 Score: 83.9593 bits (206), Expect = 7.732e-18 Identity = 62/180 (34.44%), Postives = 92/180 (51.11%), Query Frame = 1
Query: 475 TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQY-PGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
T L +A G +IVE L+ GV ++ D TPLH+AA+ G +VK + +G L +V+ NG T L A +K + EI + + DP D L++ P H A+ G+ IQ LL K S + N D +G T LH AC ++ K L+ GASI+I+ ++T + I
Sbjct: 42 TALHWACSAGHTNIVEFLLDLGVEVNLQDDASWTPLHIAASAGREDIVKALISKGAQ-----LNSVNQNGCTPLHYAASKDRYEIALMLLENGADPNA---TDKLEWTPLHRASAKGNYRLIQ-LLLKQSASTNIQDCQGNTPLHLACEEERVEAAKLLVEHGASIYIENKEEKTPLQIA 212
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Medaka
Match: ankrd28b (ankyrin repeat domain 28 [Source:NCBI gene;Acc:101159131]) HSP 1 Score: 86.2705 bits (212), Expect = 5.248e-17 Identity = 87/312 (27.88%), Postives = 139/312 (44.55%), Query Frame = 1
Query: 76 DDARFKRAVKDIDPVEIRKIIMEDTDLSL--------LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIW-TAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDI-VKWLLSKGASIWIKT 981
D +A+ ++DP E+R +I + D++ LH AA G I+ LI + DN W T H AV + ++ L+ +V N D T LH+AAA+ C L LL N++ R + T L +AA+ G + +V LL+ G + D R +H AA GH+ +VK G +T D +T L A + +VK++ D D + + P H+A NG + + L+E G+ VN + +G+ LH S H + ++ L+ GA + IK+
Sbjct: 8 DQPPLLKAIFNVDPDEVRSLIFKKEDVNAQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDNK---WLTPLHRAVASCSEEAVQVLLKHSADV-NARDKNWQTPLHIAAANKAVRCAEAL---VPLLSNVNVSDRAGR-TALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAE-----VTCKDKKSYTPLHAAASSGMISVVKFLLDLGVDINEPNAYGNT--PLHVACYNGQDVVVNELIECGA-HVNQVNEKGFAPLHFTAASRHGALCLELLVCNGADVNIKS 303 HSP 2 Score: 73.9442 bits (180), Expect = 3.682e-13 Identity = 80/284 (28.17%), Postives = 120/284 (42.25%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDN-NAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAA--HGRYHCMHYLANFTRLLPNLDAQCRNMQW-TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFL-------EEGCHDIHK-----------CLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPR-TWE--------LFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACIS 921
LH AA G + +V FL+D D++ NA T HVA G+D + LI V N+ + +G LH AA HG C+ L + D ++ TPL A HGR S + +I +G D D +G TPLH+AA GH L+ + + G H + C + ++GF + + D+ + + +W+ +F + P H AA N + + L+ G+ +VND D RG T LH A S
Sbjct: 210 LHAAASSGMISVVKFLLD---LGVDINEPNAYGNTPLHVACYNGQDVVVNELIECGAHV-NQVNEKGFAPLHFTAASRHGAL-CLELL-----VCNGADVNIKSKDGKTPLHMTAIHGRFSRSQAIIENGAEIDCEDKNGNTPLHIAARYGHELLINTLITNRADTAKRGIHGMFPLHLAALSGFSDCCRKLLSSGF------DIDTPDDFGRTCLHAAAESNLSWKPTLFFSCFIFSQFRTPLHYAAANCNYQCLFALVGSGA-SVNDLDERGCTPLHYAAAS 476 HSP 3 Score: 73.1738 bits (178), Expect = 6.380e-13 Identity = 77/293 (26.28%), Postives = 128/293 (43.69%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDA-CLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLAN-FTRLLPNLDAQCRNM--------QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
LH+AA + + L+ +DLD T H A D CLE L+ R D++G +H A+A+G C+ + N F P ++ ++ +PL AA+HG +E+L++ + DV + GRTPL LAA +GH+ V + +G + K T A K + K K ++ + K+ R L +A ++G + LL KG+ +V D G T LH+ ++GH + ++ LL AS ++ T + +
Sbjct: 437 LHYAAANCNYQCLFALVGSGASVNDLDERG--CTPLHYAAASDTDGKCLEYLLRNDANPGIR-DNQGYNAVHYASAYGHRLCLELVRNAFPPCYPLMETSGTDILNDSDVRAPVSPLHLAAYHGHHQAMEVLVQSLLDLDVRNSQGRTPLDLAAFKGHVECVDVLINQGASILVKDFTLKRTPIHAAGKNCK-KEKTKLPGLCNLRLKNMRNANGLTPLM----LAVLSGHTDCVYSLLNKGA-SVEAKDKWGRTALHRGAVTGHEECMEALLQHNASFLVRDCRGRTPVHLA 720 HSP 4 Score: 62.003 bits (149), Expect = 1.573e-9 Identity = 69/235 (29.36%), Postives = 96/235 (40.85%), Query Frame = 1
Query: 262 TAAHVAVIGGKDACLESLIMRKPEVINRP---DSRGCTLLHLAAAHGRYHC-----MHYLANFTR--------------LLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFD-DLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYT 897
T H+A G L L+ V P DS G T LH A +G+Y + + A F R L P + TPL AA+ + ++LL+ H + D G++PL +AA G V+ + D LT D TAL LA +K + I +K D + LQ P H+AA NG + +Q LL KG+ +V D GYT
Sbjct: 715 TPVHLAAACGHIGVLGGLLHAAQSVETLPVLTDSHGYTPLHWACYNGKYQMDLLLDISFFA-FLRIRDNEGAAEMLIDTLGPTIVNAKDGKNRTPLHAAAFTDHVECLQLLLSHNAQVNTVDAAGKSPLMMAAENGQTNAVELLVSSAKAD----LTIQDAVKNTALHLACSKGHETSALLILEKISDRNLINATNAALQTPLHVAARNGLTVVVQELLAKGA-SVLAVDENGYT 943 HSP 5 Score: 56.9954 bits (136), Expect = 6.391e-8 Identity = 56/218 (25.69%), Postives = 87/218 (39.91%), Query Frame = 1
Query: 340 NRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEI--------VKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
N ++ G T L LA G C++ L N + D R T L A G +E L++H + V D GRTP+HLAAA GH+ ++ L + D++G+T L A K ++ ++R ++ + L D L G VN D + T LH A + H++ ++ LLS A +
Sbjct: 641 NMRNANGLTPLMLAVLSGHTDCVYSLLNKGASVEAKDKWGR----TALHRGAVTGHEECMEALLQHNASFLVRDCRGRTPVHLAAACGHIGVLGGLLHAA-QSVETLPVLTDSHGYTPLHWACYNGKYQMDLLLDISFFAFLRIRDNEGAAEMLIDTL----------------------GPTIVNAKDGKNRTPLHAAAFTDHVECLQLLLSHNAQV 831
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Medaka
Match: ripk4 (receptor interacting serine/threonine kinase 4 [Source:NCBI gene;Acc:101174941]) HSP 1 Score: 85.8853 bits (211), Expect = 5.529e-17 Identity = 79/273 (28.94%), Postives = 124/273 (45.42%), Query Frame = 1
Query: 142 EDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEV-INRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSK 957
++ + LHWAA G I L+D+ ++ D T AHVA G++ + L+ R +V I D+ T LH AA G + L + ++D Q + + T L A+ G+ + +LI G + +T V TPLH+AA GH + ++ D+H A + G T L LA + VK + + DP L P H+AA +G ++ LL A + TD G + LH A +GH +++K LL +
Sbjct: 506 DEDQFTALHWAAQNGDEAIARLLLDRGAAINETDGQGR--TPAHVACQHGQENVIRVLLSRGADVQIKGKDN--WTALHFAAWQGHLGIVKLLVK--QAGADVDGQTSDGR-TSLHLASQRGQYRVARILIELGASVHLTSVGLNTPLHVAAETGHTSTSRLLIKHKA-DMH----AQNTQGLTPLHLACQRGHLATVKMLIAEGADPS--RPSQTLCTPCHLAAGSGHCEVLKELLLHCPDAGSVTDEEGLSPLHLAVGAGHSNVIKMLLPQ 764 HSP 2 Score: 65.4698 bits (158), Expect = 1.214e-10 Identity = 65/237 (27.43%), Postives = 103/237 (43.46%), Query Frame = 1
Query: 352 SRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTP---------------------------------LHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
+RG T LHLA + L R N++A+ + Q+T L +AA +G +I LL+ G A + TD GRTP LH AA +GHL +VK +++ D+ ++G T+L LA + + + + + + + L P H+AA G + LL K ++ + +G T LH AC GH+ VK L+++GA
Sbjct: 473 TRGSTPLHLATEKHQKPLAELL--LGRRSMNVNAKDED-QFTALHWAAQNGDEAIARLLLDRGAAINETDGQGRTPAHVACQHGQENVIRVLLSRGADVQIKGKDNWTALHFAAWQGHLGIVKLLVKQAGADV----DGQTSDGRTSLHLASQRGQYRVARILIELGASVHLTSV--GLNTPLHVAAETGH-TSTSRLLIKHKADMHAQNTQGLTPLHLACQRGHLATVKMLIAEGA 699 HSP 3 Score: 53.9138 bits (128), Expect = 5.007e-7 Identity = 42/137 (30.66%), Postives = 61/137 (44.53%), Query Frame = 1
Query: 568 GRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIK 978
G TPLHLA + PL + L +++ A D + FTAL H AA NGD + LL++G+ A+N+TD +G T H AC G ++++ LLS+GA + IK
Sbjct: 475 GSTPLHLATEKHQKPLAELLLGRRSMNVN----AKDEDQFTAL-----------------------------------HWAAQNGDEAIARLLLDRGA-AINETDGQGRTPAHVACQHGQENVIRVLLSRGADVQIK 571
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Medaka
Match: ankrd28b (ankyrin repeat domain 28 [Source:NCBI gene;Acc:101159131]) HSP 1 Score: 85.8853 bits (211), Expect = 6.564e-17 Identity = 87/312 (27.88%), Postives = 139/312 (44.55%), Query Frame = 1
Query: 76 DDARFKRAVKDIDPVEIRKIIMEDTDLSL--------LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIW-TAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDI-VKWLLSKGASIWIKT 981
D +A+ ++DP E+R +I + D++ LH AA G I+ LI + DN W T H AV + ++ L+ +V N D T LH+AAA+ C L LL N++ R + T L +AA+ G + +V LL+ G + D R +H AA GH+ +VK G +T D +T L A + +VK++ D D + + P H+A NG + + L+E G+ VN + +G+ LH S H + ++ L+ GA + IK+
Sbjct: 8 DQPPLLKAIFNVDPDEVRSLIFKKEDVNAQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDNK---WLTPLHRAVASCSEEAVQVLLKHSADV-NARDKNWQTPLHIAAANKAVRCAEAL---VPLLSNVNVSDRAGR-TALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAE-----VTCKDKKSYTPLHAAASSGMISVVKFLLDLGVDINEPNAYGNT--PLHVACYNGQDVVVNELIECGA-HVNQVNEKGFAPLHFTAASRHGALCLELLVCNGADVNIKS 303 HSP 2 Score: 77.411 bits (189), Expect = 3.042e-14 Identity = 76/282 (26.95%), Postives = 128/282 (45.39%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDA-CLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNM--------QWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFD-DLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIK 978
LH+AA + + L+ +DLD T H A D CLE L+ R D++G +H A+A+G C+ +A+ T L ++ ++ +PL AA+HG +E+L++ + DV + GRTPL LAA +GH+ V + +G + K T T + A T E ++ + ++ D + Q P +A ++G + LL KG+ +V D G T LH+ ++GH + ++ LL AS ++
Sbjct: 442 LHYAAANCNYQCLFALVGSGASVNDLDERG--CTPLHYAAASDTDGKCLEYLLRNDANPGIR-DNQGYNAVHYASAYGHRLCLELIASETPLDVLMETSGTDILNDSDVRAPVSPLHLAAYHGHHQAMEVLVQSLLDLDVRNSQGRTPLDLAAFKGHVECVDVLINQGASILVKDFTLKR----TPIHAAATNGHSECLRLLIGNADLQSAVDIQDGNGQTPLMLAVLSGHTDCVYSLLNKGA-SVEAKDKWGRTALHRGAVTGHEECMEALLQHNASFLVR 715 HSP 3 Score: 71.633 bits (174), Expect = 1.798e-12 Identity = 82/321 (25.55%), Postives = 131/321 (40.81%), Query Frame = 1
Query: 136 IMEDTDL----SLLHWAALKGSVVIVNFLIDKMEFPSDLD-NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIV---------------------------------ELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQ----YPGHIAAVNGDLLNIQGLLEK-GSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
I+ D+D+ S LH AA G + L+ + DLD N++ T +A G C++ LI + ++ + + T +H AA +G C+ L L +D Q N Q TPLM A G V E L++H + V D GRTP+HLAAA GH+ ++ L + D++G+T L A D V+ + ++ E+F + P H A + + + L++ G VN D + T LH A + H++ ++ LLS A +
Sbjct: 541 ILNDSDVRAPVSPLHLAAYHGHHQAMEVLVQSLL---DLDVRNSQGRTPLDLAAFKGHVECVDVLINQGASILVKDFTLKRTPIHAAATNGHSECLRLLIGNADLQSAVDIQDGNGQ-TPLMLAVLSGHTDCVYSLLNKGASVEAKDKWGRTALHRGAVTGHEECMEALLQHNASFLVRDCRGRTPVHLAAACGHIGVLGGLLHAA-QSVETLPVLTDSHGYTPLHWACYNGHDTCVEVLLEQ-------EVFHKAEGNPFSPLHCAVIRDNEGAAEMLIDTLGPTIVNAKDGKNRTPLHAAAFTDHVECLQLLLSHNAQV 849 HSP 4 Score: 70.4774 bits (171), Expect = 3.761e-12 Identity = 83/285 (29.12%), Postives = 119/285 (41.75%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDN-NAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAA--HGRYHCMHYLANFTRLLPNLDAQCRNMQW-TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFL-------EEGCHDIHK-----------CLTAVDNNGF----------TALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACIS 921
LH AA G + +V FL+D D++ NA T HVA G+D + LI V N+ + +G LH AA HG C+ L + D ++ TPL A HGR S + +I +G D D +G TPLH+AA GH L+ + + G H + C + ++GF T L A E + + + D + F + P H AA N + + L+ G+ +VND D RG T LH A S
Sbjct: 210 LHAAASSGMISVVKFLLD---LGVDINEPNAYGNTPLHVACYNGQDVVVNELIECGAHV-NQVNEKGFAPLHFTAASRHGAL-CLELL-----VCNGADVNIKSKDGKTPLHMTAIHGRFSRSQAIIENGAEIDCEDKNGNTPLHIAARYGHELLINTLITNRADTAKRGIHGMFPLHLAALSGFSDCCRKLLSSGFDIDTPDDFGRTCLHAAAAGGNLECLNLLLNTGADFNRKDSFG--RTPLHYAAANCNYQCLFALVGSGA-SVNDLDERGCTPLHYAAAS 481 HSP 5 Score: 63.5438 bits (153), Expect = 5.106e-10 Identity = 67/250 (26.80%), Postives = 106/250 (42.40%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLD--NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFD-DLQYPGHIAAVNGDLLNIQGLLEKG-SIAVNDTDNRGYTM 900
+H AA G + ++ L+ + L ++ +T H A G D C+E L+ + EV ++ + + LH A L L P + TPL AA+ + ++LL+ H + D G++PL +AA G V+ + D LT D TAL LA +K + I +K D + LQ P H+AA NG + +Q LL KG S+ D + + +T
Sbjct: 723 VHLAAACGHIGVLGGLLHAAQSVETLPVLTDSHGYTPLHWACYNGHDTCVEVLLEQ--EVFHKAEGNPFSPLHCAVIRDNEGAAEML--IDTLGPTIVNAKDGKNRTPLHAAAFTDHVECLQLLLSHNAQVNTVDAAGKSPLMMAAENGQTNAVELLVSSAKAD----LTIQDAVKNTALHLACSKGHETSALLILEKISDRNLINATNAALQTPLHVAARNGLTVVVQELLAKGASVLAVDENGKHFTF 964 HSP 6 Score: 56.9954 bits (136), Expect = 5.504e-8 Identity = 85/326 (26.07%), Postives = 124/326 (38.04%), Query Frame = 1
Query: 76 DDARFKRAVKDIDPV--EIRKIIMEDTDL--SLLHWAALKGSVVIVNFLIDKMEFPSDLD-NNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATD----VTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRD------------KEKDPRTWELFDD----LQY-----------------PGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGH 927
D A FK V+ +D + + I+++D L + +H AA G + LI + S +D + T +AV+ G C+ SL + K + D G T LH A G CM L L CR TP+ AA G I ++ L+ + + +TD G TPLH A GH V+ LE+ HK + N F+ L A + + + + D K + P F D LQ P +AA NG ++ L+ + D T LH AC GH
Sbjct: 588 DLAAFKGHVECVDVLINQGASILVKDFTLKRTPIHAAATNGHSECLRLLIGNADLQSAVDIQDGNGQTPLMLAVLSGHTDCVYSL-LNKGASVEAKDKWGRTALHRGAVTGHEECMEALLQHNASF--LVRDCRGR--TPVHLAAACGHIGVLGGLLHAAQSVETLPVLTDSHGYTPLHWACYNGHDTCVEVLLEQ--EVFHK----AEGNPFSPLHCAVIRDNEGAAEMLIDTLGPTIVNAKDGKNRTPLHAAAFTDHVECLQLLLSHNAQVNTVDAAGKSPLMMAAENGQTNAVELLVSSAKADLTIQDAVKNTALHLACSKGH 902
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Medaka
Match: ankrd44 (ankyrin repeat domain 44 [Source:NCBI gene;Acc:101173198]) HSP 1 Score: 85.8853 bits (211), Expect = 6.636e-17 Identity = 82/274 (29.93%), Postives = 128/274 (46.72%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLID---KMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAA--HGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
LH AA G + +V L+ +++ P+ N TA H+A G+DA + LI V ++P+++G T LH AAA HG C+ +L N +++ Q R+ + +PL A HGR + + LI++G + D DG TPLH+AA GH L+ + G + + +G L LA + + + + T + H AA G++ +Q LL+ G N TD G T LH A S H ++ L+ G I
Sbjct: 210 LHAAASSGQIAVVKHLLSLSVEIDEPNAFGN-----TALHLACFNGQDAVVSELIDYGANV-SQPNNKGFTPLHFAAASTHGAL-CLEFLVNNGA---DINIQSRDGK-SPLHMTAVHGRFTRSQTLIQNGGEINSVDKDGNTPLHVAARYGHELLINTLITSGADCARRGV-----HGMFPLHLAALNAHSDCCRKLLSSGFQIDTPDSLGRTCL--HAAAAGGNVDCVQLLLKSGG-EHNRTDKCGRTPLHYAAASRHYHCLETLVVCGTDI 464 HSP 2 Score: 77.411 bits (189), Expect = 2.779e-14 Identity = 69/224 (30.80%), Postives = 102/224 (45.54%), Query Frame = 1
Query: 301 CLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFD-DLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
CLE L+ + D +G + +H AAA G HC+ L + T +A C N Q +PL AA+HG +++L+R G D D GRTPL LAA GH V L +G L+ G T + LA V+ + D+ ++ D + P +A V G + + LLE+ VN D +G LH A + + V+ LL + A +
Sbjct: 509 CLEFLLQSGAAAALK-DKQGYSPVHYAAAFGHRHCLELLLD-THDGRQEEADCGNAQ-SPLHLAAYHGHAQALQVLLRGGREVDERDQAGRTPLALAALRGHADCVHTLLSQGASP----LSTDQLYGRTPVHLAVMNGHTTCVRLLLDEPDGAEVLDVADPQGRTPLMLAVVGGHVDAVSLLLER-EANVNTADKKGLMALHLALLCAQDECVQCLLEQDACL 724 HSP 3 Score: 72.7886 bits (177), Expect = 8.281e-13 Identity = 95/370 (25.68%), Postives = 144/370 (38.92%), Query Frame = 1
Query: 55 FAYITADDDARFKRAVKDIDPVEIRKIIMEDTDLSLL--------HWAALKGSVVIVNFLIDKMEFPSDLDNNAEIW-TAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHC---------------------MHYLA--NFTRLLPNLDAQCRNMQ------WTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEE----------GCHDIH-KCLTAVD-----------------NNGFTALKLAETKSKDEI-VKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGA 963
A + D +A+ DP EIR +I + D+++L H AA G I LI + DN +W T H AV ++ LI R +N D T LH+AAA+ C +H+ A T ++ L A+ N+ L +AA+ G + +V +L+ HG D G TPLH AA+ G + +VK+ L G +H C D N GFT L A + + ++++ + D D + P H+ AV+G Q L++ G +N D G T LH A GH ++ L++ GA
Sbjct: 1 MAVLKLADQPALIQAIFSGDPEEIRMLIYKSEDINVLDAEKRTPLHAAAFLGDAEITELLILSGARVNAKDN---MWFTPLHRAVASRSQDAVQVLI-RHSADVNARDKNWQTPLHVAAANNALRCAEVIIPLLTSVNVSDRGGRTALHHAALNGHTEMVNLLLAKGANINAFDKKDGRALHWAAFMGHLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQIAVVKHLLSLSVEIDEPNAFGNTALHLACFNGQDAVVSELIDYGANVSQPNNKGFTPLHFAAASTHGALCLEFLVNNGADINIQS--RDGKSPLHMTAVHGRFTRSQTLIQNGG-EINSVDKDGNTPLHVAARYGHELLINTLITSGA 363 HSP 4 Score: 70.4774 bits (171), Expect = 3.751e-12 Identity = 74/266 (27.82%), Postives = 114/266 (42.86%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLI-----DKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGC--------TLLHLAAAHGRYHCMHYLANFTRLLPNLDAQ------CRNMQW-TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFD-DLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYT 897
+H AA +G ++ L+ + P D + +T H A G+D C+E L+ + RGC T LH A +G C L LDA CR+ + TPL AA+ G+ ++LL+ H D D GRTPL AAA+G + ++ L+ G L+ +D +G T L +A + ++ V I + D + +Q P H+AA G +Q LL +G+ + D G T
Sbjct: 735 IHLAAAQGHASWLSELLTIACSESSSVPPLKDGSG--YTPLHWACFYGQDGCVEVLL----------EQRGCRCIDGNPFTPLHCAVVNGHESCAALL---------LDAMGSDITGCRDAKGRTPLHAAAFAGQTECIQLLLSHDAPVDPADDHGRTPLMTAAAKGRVGALEALLDAG-----AGLSLIDRDGNTPLHVACSNGTEDGVLMILQRLSDATLINATNAAMQTPLHMAAQRGLKKAVQELLSRGA-STQTVDEDGLT 973 HSP 5 Score: 69.707 bits (169), Expect = 6.497e-12 Identity = 78/279 (27.96%), Postives = 118/279 (42.29%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIW--TAAHVAVIGGKDACLESLIMRKP---EVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLM---YAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQY-PGHIAAVNGDLLNIQGLLEK-GSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
L AAL+G V+ L+ + P D +++ T H+AV+ G C+ L++ +P EV++ D +G T L LA G + LL +A LM A + V+ L+ D GRTP+HLAAA+GH + L C + D +G+T L A +D V+ + ++ R D + P H A VNG LL+ GS D +G T LH A +G + ++ LLS A +
Sbjct: 599 LALAALRGHADCVHTLLSQGASPLSTD---QLYGRTPVHLAVMNGHTTCVR-LLLDEPDGAEVLDVADPQGRTPLMLAVVGGHV-------DAVSLLLEREANVNTADKKGLMALHLALLCAQDECVQCLLEQDACLLQGDSQGRTPIHLAAAQGHASWLSELLTIACSESSSVPPLKDGSGYTPLHWACFYGQDGCVEVLLEQ----RGCRCIDGNPFTPLHCAVVNGHESCAALLLDAMGSDITGCRDAKGRTPLHAAAFAGQTECIQLLLSHDAPV 862 HSP 6 Score: 67.3958 bits (163), Expect = 3.258e-11 Identity = 73/255 (28.63%), Postives = 103/255 (40.39%), Query Frame = 1
Query: 271 HVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAA---------------EG--------HLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGAS 966
H+A + C L+ + I+ PDS G T LH AAA G C+ L D R TPL YAA +E L+ G + TD GR+ LH AAA EG +++ L+ G K D G++ + A ++ + D + + Q P H+AA +G +Q LL +G V++ D G T L A + GH D V LLS+GAS
Sbjct: 377 HLAALNAHSDCCRKLLSSGFQ-IDTPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGR----TPLHYAAASRHYHCLETLVVCGTDINATDQWGRSALHYAAASDLDRRRRVALEPDSEGVQAEKEKEAAQCLEFLLQSGAAAALK-----DKQGYSPVHYAAAFGHRHCLELLLDTHDGRQEEADCGNAQSPLHLAAYHGHAQALQVLL-RGGREVDERDQAGRTPLALAALRGHADCVHTLLSQGAS 620 HSP 7 Score: 63.5438 bits (153), Expect = 5.850e-10 Identity = 81/309 (26.21%), Postives = 131/309 (42.39%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANF----TRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRH-------------------------------GVATDVT---DVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFD-DLQYPGHIAAVNGDLLNIQGLLEKGSIA--VNDTDNRGYTMLHKACISGHIDIVKWLLSKGAS 966
L A + G V V+ L+++ + D A H+A++ +D C++ L+ + ++ + DS+G T +HLAAA G + L + +P L +TPL +A ++G+ VE+L+ + +D+T D GRTPLH AA G ++ L HD + D++G T L A K + ++ + D L D D P H+A NG + +L++ S A +N T+ T LH A G V+ LLS+GAS
Sbjct: 669 LMLAVVGGHVDAVSLLLEREANVNTADKKG--LMALHLALLCAQDECVQCLLEQDACLL-QGDSQGRTPIHLAAAQGHASWLSELLTIACSESSSVPPLK---DGSGYTPLHWACFYGQDGCVEVLLEQRGCRCIDGNPFTPLHCAVVNGHESCAALLLDAMGSDITGCRDAKGRTPLHAAAFAGQTECIQLLLS---HDAP--VDPADDHGRTPLMTAAAKGRVGALEALLDAGAG---LSLIDRDGNTPLHVACSNGTEDGVLMILQRLSDATLINATNAAMQTPLHMAAQRGLKKAVQELLSRGAS 963
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Planmine SMEST
Match: SMESG000036685.1 (SMESG000036685.1) HSP 1 Score: 1174.84 bits (3038), Expect = 0.000e+0 Identity = 641/641 (100.00%), Postives = 641/641 (100.00%), Query Frame = 1
Query: 7 MLNDEYLEESEKRRLLFAYITADDDARFKRAVKDIDPVEIRKIIMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIVXXXXXXXXXXXXXXXXXXXXPFLKKEDPDRRVFIXXXXXXXXXXXXXXXYKRLNELIEESKSEILNLEEMINKKEMILQLEQSEVXXXXXXXXXXLIKQKHRNIKALKYEIMKLNYQLNVVKFNLYQLTKEPTIKEREIGRALADCEHQFEEITDRLFYEREVREQTEAELSRIRQQLLKLRDFILKNDCPDDLVTMVIAFDKYLIGKENLTVIRKAPEKKDKVYSIQFEINDLSKITISKVSYYTDSKLIVKSNIENLSFKKFVEYIKCMIKQDMKRHXXXXXXXXXXXXXXHLHPKGGIFVTIK 1929
MLNDEYLEESEKRRLLFAYITADDDARFKRAVKDIDPVEIRKIIMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIVKRKGHGKLLKLLEAEEARYEPFLKKEDPDRRVFIPLLSSLDNPNLDSLDYKRLNELIEESKSEILNLEEMINKKEMILQLEQSEVDDETDDTDDDLIKQKHRNIKALKYEIMKLNYQLNVVKFNLYQLTKEPTIKEREIGRALADCEHQFEEITDRLFYEREVREQTEAELSRIRQQLLKLRDFILKNDCPDDLVTMVIAFDKYLIGKENLTVIRKAPEKKDKVYSIQFEINDLSKITISKVSYYTDSKLIVKSNIENLSFKKFVEYIKCMIKQDMKRHKIKKGNKGKQIKGKHLHPKGGIFVTIK
Sbjct: 1 MLNDEYLEESEKRRLLFAYITADDDARFKRAVKDIDPVEIRKIIMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIVKRKGHGKLLKLLEAEEARYEPFLKKEDPDRRVFIPLLSSLDNPNLDSLDYKRLNELIEESKSEILNLEEMINKKEMILQLEQSEVDDETDDTDDDLIKQKHRNIKALKYEIMKLNYQLNVVKFNLYQLTKEPTIKEREIGRALADCEHQFEEITDRLFYEREVREQTEAELSRIRQQLLKLRDFILKNDCPDDLVTMVIAFDKYLIGKENLTVIRKAPEKKDKVYSIQFEINDLSKITISKVSYYTDSKLIVKSNIENLSFKKFVEYIKCMIKQDMKRHKIKKGNKGKQIKGKHLHPKGGIFVTIK 641
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Planmine SMEST
Match: SMESG000005009.1 (SMESG000005009.1) HSP 1 Score: 174.866 bits (442), Expect = 2.176e-47 Identity = 108/315 (34.29%), Postives = 167/315 (53.02%), Query Frame = 1
Query: 97 AVKDIDPVEIRKIIMEDTDL---------SLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNN-GFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWIKTLGQETVIDIV 1011
AVK+ D E++K++ + + LHWAA G++ +++L+ P+ WT AHVA I G+DAC++ LI +N D+RG T HLA+ HG + + ++D Q WTP+ AA+HGR+ ++ L+R G+ +D D G T HLAA EGHLP++K+ + +G C+ N+ G T LA+ K +IV+YI E + E L +P H+AA N DL ++ L+E G + +N+ DN G T LHKA G++ +++WL+ GA+ I+ ET DI
Sbjct: 12 AVKNGDTEEMKKMVKSGASINEVDSEHKFTPLHWAAYVGALECMHWLMWNGADPTVCTPRG--WTPAHVASIKGQDACIQCLI-NNGVGLNSKDTRGSTPAHLASVHGNSFTLQAILRAGVETESVDYQG----WTPVHGAAFHGRLGCLQFLLRWGLRSDDVDNQGNTAAHLAAMEGHLPVLKFLVAQG--PTSSCVLGCRNDDGETPKMLAQRFFKQDIVEYISHLEWERDCPEEAMSLAFPAHLAAANDDLEQLRALIENGVVNINERDNHGSTPLHKAAGQGNLKVIQWLIELGANQEIQNDAGETAQDIA 317
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Planmine SMEST
Match: SMESG000026756.1 (SMESG000026756.1) HSP 1 Score: 90.8929 bits (224), Expect = 1.175e-18 Identity = 87/302 (28.81%), Postives = 125/302 (41.39%), Query Frame = 1
Query: 136 IMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCH----------DIHK------------------CLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFD-DLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLS 954
+ + ++L +H+A L GS IV LID+ + S LD + H A G LE L+ R V N D R + +H AA +G + L + D P+ YA +G +++V LLI G + ++ D D R P+H A H+ +VK L+ C+ IH CL A G + A DEIVK + + + EL D D H A G L NI +L S +N D +H AC +GH +IVK L+S
Sbjct: 127 VHDKSNLRPIHYACLSGSYQIVEMLIDRCQIES-LDKHD--MMPLHYASQNGHCGVLELLLSRVSNV-NAVDKRSQSPIHYAAQNGHVDAVSLLLKHNSAIEAPD----KFDRCPIHYACQNGHLNVVRLLISEGCSIEICDKDKRKPIHFACQNDHVEIVKLLLKSKCNLEASDKNGLRPIHYACFHGNVEIVKLLISSQCCLDAESLTGSRPIHFASFYGHDEIVKILLSERCN---IELQDKDSGRAIHYACCRGHL-NIVEMLLAQSCEINAGDKNYRRPIHHACQNGHCEIVKLLVS 416 HSP 2 Score: 57.3806 bits (137), Expect = 2.680e-8 Identity = 63/242 (26.03%), Postives = 101/242 (41.74%), Query Frame = 1
Query: 331 EVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGC----HD------IH-KCLT----------------AVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWI 975
EV + D R +H A G Y + L ++ N++ C P+ YA +G IVELL+ + + D + PLH A GH +V+ L C HD IH CL+ ++D + L A +++ + + + + Q P H AA NG + + LL+ S A+ D +H AC +GH+++V+ L+S+G SI I
Sbjct: 27 EVFCKNDKRP---IHYACESGDYDIVKLLLSYK---CNIEV-CDINLMRPIHYACQNGYHKIVELLLSNQADKEAKDKNNGRPLHHACRSGHANVVELLLNYYCDIEVHDKSNLRPIHYACLSGSYQIVEMLIDRCQIESLDKHDMMPLHYASQNGHCGVLELLLSRVSNVNAVD--KRSQSPIHYAAQNGHVDAVSLLLKHNS-AIEAPDKFDRCPIHYACQNGHLNVVRLLISEGCSIEI 258 HSP 3 Score: 53.9138 bits (128), Expect = 3.221e-7 Identity = 49/196 (25.00%), Postives = 87/196 (44.39%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRN-MQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRD 747
+H+A+ G IV L+ + D ++ A H A G +E L+ + E IN D +H A +G + L ++ D + R+ + TP+ YA ++G ++ +L+ + +V D D P+H A GHL +VK + + C+ + A ++ L LA K +IV+ + D
Sbjct: 333 IHFASFYGHDEIVKILLSERCNIELQDKDS--GRAIHYACCRGHLNIVEMLLAQSCE-INAGDKNYRRPIHHACQNGHCEIVKLLVSYK-----CDKEVRDENKLTPIHYACFYGYFDVMTVLLINNFKVEVGDKDDGRPIHYACFFGHLHIVKILISQNCN-----IEACTKENWSPLDLASQKGFSQIVELLSD 515
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Planmine SMEST
Match: SMESG000026756.1 (SMESG000026756.1) HSP 1 Score: 90.5077 bits (223), Expect = 1.394e-18 Identity = 87/302 (28.81%), Postives = 125/302 (41.39%), Query Frame = 1
Query: 136 IMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCH----------DIHK------------------CLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFD-DLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLS 954
+ + ++L +H+A L GS IV LID+ + S LD + H A G LE L+ R V N D R + +H AA +G + L + D P+ YA +G +++V LLI G + ++ D D R P+H A H+ +VK L+ C+ IH CL A G + A DEIVK + + + EL D D H A G L NI +L S +N D +H AC +GH +IVK L+S
Sbjct: 133 VHDKSNLRPIHYACLSGSYQIVEMLIDRCQIES-LDKHD--MMPLHYASQNGHCGVLELLLSRVSNV-NAVDKRSQSPIHYAAQNGHVDAVSLLLKHNSAIEAPD----KFDRCPIHYACQNGHLNVVRLLISEGCSIEICDKDKRKPIHFACQNDHVEIVKLLLKSKCNLEASDKNGLRPIHYACFHGNVEIVKLLISSQCCLDAESLTGSRPIHFASFYGHDEIVKILLSERCN---IELQDKDSGRAIHYACCRGHL-NIVEMLLAQSCEINAGDKNYRRPIHHACQNGHCEIVKLLVS 422 HSP 2 Score: 58.151 bits (139), Expect = 1.974e-8 Identity = 69/273 (25.27%), Postives = 114/273 (41.76%), Query Frame = 1
Query: 244 NNAEIWTAAHVAVIGGKDACLESLIMRKP--EVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGC----HD------IH-KCLT----------------AVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASIWI 975
N+ ++ H A G+ ++ L+M EV + D R +H A G Y + L ++ N++ C P+ YA +G IVELL+ + + D + PLH A GH +V+ L C HD IH CL+ ++D + L A +++ + + + + Q P H AA NG + + LL+ S A+ D +H AC +GH+++V+ L+S+G SI I
Sbjct: 2 NDNDLMRPIHYACQYGQLDLVKMLLMGMCDVEVFCKNDKRP---IHYACESGDYDIVKLLLSYK---CNIEV-CDINLMRPIHYACQNGYHKIVELLLSNQADKEAKDKNNGRPLHHACRSGHANVVELLLNYYCDIEVHDKSNLRPIHYACLSGSYQIVEMLIDRCQIESLDKHDMMPLHYASQNGHCGVLELLLSRVSNVNAVD--KRSQSPIHYAAQNGHVDAVSLLLKHNS-AIEAPDKFDRCPIHYACQNGHLNVVRLLISEGCSIEI 264 HSP 3 Score: 53.9138 bits (128), Expect = 3.229e-7 Identity = 49/196 (25.00%), Postives = 87/196 (44.39%), Query Frame = 1
Query: 163 LHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRN-MQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRD 747
+H+A+ G IV L+ + D ++ A H A G +E L+ + E IN D +H A +G + L ++ D + R+ + TP+ YA ++G ++ +L+ + +V D D P+H A GHL +VK + + C+ + A ++ L LA K +IV+ + D
Sbjct: 339 IHFASFYGHDEIVKILLSERCNIELQDKDS--GRAIHYACCRGHLNIVEMLLAQSCE-INAGDKNYRRPIHHACQNGHCEIVKLLVSYK-----CDKEVRDENKLTPIHYACFYGYFDVMTVLLINNFKVEVGDKDDGRPIHYACFFGHLHIVKILISQNCN-----IEACTKENWSPLDLASQKGFSQIVELLSD 521
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Planmine SMEST
Match: SMESG000078033.1 (SMESG000078033.1) HSP 1 Score: 88.9669 bits (219), Expect = 5.833e-18 Identity = 83/283 (29.33%), Postives = 131/283 (46.29%), Query Frame = 1
Query: 133 IIMEDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDN-NAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEE---GCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELFDDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
+I E + SLLH+A L+ IV+FL + F D++ A H+A G +E L K I+ D +G T LH A G + + L + + L+ ++ P+ +V++L+ +G +V + +G+TPL +AA EGH L L E +I L DN+GF+ L A EI+K D + +D + H AA+ G + L+E S +N TDN T +H A ++ ++ +VK LL KGA+I
Sbjct: 75 VIDEKNNFSLLHYACLENHKDIVSFL---LIFKPDVNVIGANKIRPIHLAAQQGHMEIVELLCKYKSTHIDAADVKGQTPLHTACRRGHLNVVKILLKYQASVQKLELDSISVMHHPVR----RNDTDMVKVLVAYGSPLNVRNNNGQTPLMVAAIEGHFELFDLLLNEMKKQGENIISILNEKDNDGFSCLSHAVQNENSEIIKICIGNAVDLDAKQ--NDGKTIFHSAAIIG-YSELFDLIETNSYHLNITDNELNTPIHHAIMNNNLKMVKLLLDKGANI 347 HSP 2 Score: 63.1586 bits (152), Expect = 5.061e-10 Identity = 60/204 (29.41%), Postives = 91/204 (44.61%), Query Frame = 1
Query: 142 EDTDLSLLHWAALKGSVVIVNFLIDKMEFPSDLDNNAEIWTAAHVAVIGGKDACLESLIMRKPEVINRPDSRGCTLLHLAAAHGRYHCMHYLANFTRLLPNLDAQCRNMQWTPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKE 753
+D+ S LH AA G+ I L+ P N + A HVA I G C+ L+ E D+ + ++LAA +GRY +L + + + D RN LM+AA G + IVE L+ V ++ D T LH AA GHL +VK ++G + D G T L +A + +V++ + E
Sbjct: 428 DDSHNSPLHCAAKIGNAEICQILLKAGADPFLFSQND--FLALHVATIKGHLDCVIILLEASKETSYYRDANENSAINLAAENGRYEIFKHLVDIGKDKMSKDDFGRN----TLMFAAKSGCLPIVEDLLNDEVNINLYDQMKSTALHQAAERGHLDIVKLLWKQGAK------LSEDMFGSTQLDVAILNQQWHVVRFFLNNE 619 HSP 3 Score: 55.8398 bits (133), Expect = 1.000e-7 Identity = 51/167 (30.54%), Postives = 79/167 (47.31%), Query Frame = 1
Query: 475 TPLMYAAWHGRISIVELLIRHGVATDVTDVDGRTPLHLAAAEGHLPLVKYFLEEGCHDIHKCLTAVDNNGFTALKLAETKSKDEIVKYIRDKEKDPRTWELF--DDLQYPGHIAAVNGDLLNIQGLLEKGSIAVNDTDNRGYTMLHKACISGHIDIVKWLLSKGASI 969
+PL AA G I ++L++ G + + LH+A +GHL V LE + D N +A+ LA + EI K++ D KD + + F + L + AA +G L ++ LL + +N D T LH+A GH+DIVK L +GA +
Sbjct: 433 SPLHCAAKIGNAEICQILLKAGADPFLFSQNDFLALHVATIKGHLDCVIILLEAS----KETSYYRDANENSAINLAAENGRYEIFKHLVDIGKDKMSKDDFGRNTLMF----AAKSGCLPIVEDLLND-EVNINLYDQMKSTALHQAAERGHLDIVKLLWKQGAKL 590 The following BLAST results are available for this feature:
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Alpha-latrocrustotoxin-Lt1a vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Alpha-latrocrustotoxin-Lt1a vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Alpha-latrocrustotoxin-Lt1a vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30033645 ID=SMED30033645|Name=Alpha-latrocrustotoxin-Lt1a|organism=Schmidtea mediterranea sexual|type=transcript|length=1965bpback to top protein sequence of SMED30033645-orf-1 >SMED30033645-orf-1 ID=SMED30033645-orf-1|Name=SMED30033645-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=644bp YSMLNDEYLEESEKRRLLFAYITADDDARFKRAVKDIDPVEIRKIIMEDTback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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