General transcription factor IIH subunit 4

Overview


Name	General transcription factor IIH subunit 4
Smed ID	SMED30033635
Length (bp)	1982

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of General transcription factor IIH subunit 4 (SMED30033635) t-SNE clustered cells

Violin plots show distribution of expression levels for General transcription factor IIH subunit 4 (SMED30033635) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of General transcription factor IIH subunit 4 (SMED30033635) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30033635

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
X1 cell	SMED30033635	SMESG000022992.1	SmedASXL_008862	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Human
Match: GTF2H4 (general transcription factor IIH subunit 4 [Source:HGNC Symbol;Acc:HGNC:4658])

HSP 1 Score: 224.942 bits (572), Expect = 9.210e-66
Identity = 140/462 (30.30%), Postives = 246/462 (53.25%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTR--VSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSD 1823
            L  F+  L    +++++  P TC  +FREL +L+K+ V R+LF+++ + ++ +A WV K++S    +S   L+ L I+    +  G QG  + LN  F++NL+ AL GG      +T  L  + +  +  S     L K A ++WE VL  + G     + +  +  ++  AG +K+ E      IT+ GFQFLLL    Q+W F++ YL+  +     +++IL  LFQ+   ++ + Y+    + +    LQH  EFG +         ++ T L ++L SG             A   +   G+I++E++  +YA+T+S+LQIA++ +F ++  RFP      +T +S+ +A+  GIT+ QII FL    HP M K+  V+P T+ D+I+L E  R+R R   G +Y  F +Q +F+ L+  A     + ++N  K +++V    H  +++FWK +K+S 
Sbjct:   19 LQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQG--LILNPIFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPS-----LDKYAEERWEVVLHFMVGS-PSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVS----------GAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS 462

[Close]

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTR--VSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSD 1823
            L  F+  L    +++++  P TC  +FREL +L+K+ V R+LF+++ + ++ +A WV K++S    +S   L+ L I+    +  G QG  + LN  F++NL+ AL GG      +T  L  + +  +  S     L K A ++WE VL  + G     + +  +  ++  AG +K+ E      IT+ GFQFLLL    Q+W F++ YL+  +     +++IL  LFQ+   ++ + Y+    + +    LQH  EFG +         ++ T L ++L SG             A   +   G+I++E++  +YA+T+S+LQIA++ +F ++  RFP      +T +S+ +A+  GIT+ QII FL    HP M K+  V+P T+ D+I+L E  R+R R   G +Y  F +Q +F+ L+  A     + ++N  K +++V    H  +++FWK +K+S 
Sbjct:   19 LQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQG--LILNPIFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPS-----LDKYAEERWEVVLHFMVGS-PSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVS----------GAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS 462

[Close]

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTR--VSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSD 1823
            L  F+  L    +++++  P TC  +FREL +L+K+ V R+LF+++ + ++ +A WV K++S    +S   L+ L I+    +  G QG  + LN  F++NL+ AL GG      +T  L  + +  +  S     L K A ++WE VL  + G     + +  +  ++  AG +K+ E      IT+ GFQFLLL    Q+W F++ YL+  +     +++IL  LFQ+   ++ + Y+    + +    LQH  EFG +         ++ T L ++L SG             A   +   G+I++E++  +YA+T+S+LQIA++ +F ++  RFP      +T +S+ +A+  GIT+ QII FL    HP M K+  V+P T+ D+I+L E  R+R R   G +Y  F +Q +F+ L+  A     + ++N  K +++V    H  +++FWK +K+S 
Sbjct:   19 LQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQG--LILNPIFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPS-----LDKYAEERWEVVLHFMVGS-PSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVS----------GAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS 462

[Close]

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTR--VSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSD 1823
            L  F+  L    +++++  P TC  +FREL +L+K+ V R+LF+++ + ++ +A WV K++S    +S   L+ L I+    +  G QG  + LN  F++NL+ AL GG      +T  L  + +  +  S     L K A ++WE VL  + G     + +  +  ++  AG +K+ E      IT+ GFQFLLL    Q+W F++ YL+  +     +++IL  LFQ+   ++ + Y+    + +    LQH  EFG +         ++ T L ++L SG             A   +   G+I++E++  +YA+T+S+LQIA++ +F ++  RFP      +T +S+ +A+  GIT+ QII FL    HP M K+  V+P T+ D+I+L E  R+R R   G +Y  F +Q +F+ L+  A     + ++N  K +++V    H  +++FWK +K+S 
Sbjct:   19 LQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQG--LILNPIFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPS-----LDKYAEERWEVVLHFMVGS-PSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVS----------GAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS 462

[Close]

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTR--VSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSD 1823
            L  F+  L    +++++  P TC  +FREL +L+K+ V R+LF+++ + ++ +A WV K++S    +S   L+ L I+    +  G QG  + LN  F++NL+ AL GG      +T  L  + +  +  S     L K A ++WE VL  + G     + +  +  ++  AG +K+ E      IT+ GFQFLLL    Q+W F++ YL+  +     +++IL  LFQ+   ++ + Y+    + +    LQH  EFG +         ++ T L ++L SG             A   +   G+I++E++  +YA+T+S+LQIA++ +F ++  RFP      +T +S+ +A+  GIT+ QII FL    HP M K+  V+P T+ D+I+L E  R+R R   G +Y  F +Q +F+ L+  A     + ++N  K +++V    H  +++FWK +K+S 
Sbjct:   19 LQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQG--LILNPIFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPS-----LDKYAEERWEVVLHFMVGS-PSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVS----------GAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS 462

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Celegans
Match: Y73F8A.24 (General transcription factor IIH subunit 4 [Source:UniProtKB/TrEMBL;Acc:D3NQB3])

HSP 1 Score: 162.54 bits (410), Expect = 3.693e-43
Identity = 130/471 (27.60%), Postives = 234/471 (49.68%), Query Frame = 3

Query:  483 LLLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECMRLNKKFQENLKYA--LFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVA-------GIIHKTXXXXXXXXXXXXAGYIKNDEITNEGFQFLLLQKKKQVWQFLISYLEY-IKEPSSIIDILPLLFQI------------GCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAMKEKS----VVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKN 1817
            L L+F+ T+  +  +++   P     I+R L  +++    ++++      K    +  + + +  +   ++ L DL +  R +   +   + ++  ++ +  YA  L   +  +LV E NE N +    DV  KKA+++W+ +L  +A         + +T+ N   ++   + G  +  EIT  GFQFLLL   KQ+W ++I YL+  + +   I++++  L QI             C  ID  +T     + Q E+L H  E G I         F +T LL  L + N+ +      D SA  +  S G +I+E++  +YA+T S LQ+A++ +F ++  RF   +   IT +S+  AL  GIT+ QIISFL  N HP     S     +PIT+ D+I+L E  R R      Y+Y +F ++ EF  +  +A  +N + W + ++ +V+V ED H ++R+++K  K+
Sbjct:   22 LFLDFLITIPPKTRQRLLQKPSCAFFIYRMLPPIAQQTTIQLIW------KGSFPTASDVEETKEIEDQVKLLKDLGLVHRQTTDGK---LNIDTDYKRSYMYAAMLGAAQISSLVLEPNEGNRR--GKDV-EKKAVERWDCILRYLALPSEENTQAVSETTRNLFKKANFTSGGDTQ-IEITTFGFQFLLLSPVKQMWTYVIEYLKLEMSQGQDIVEVIEPLIQIVLLANRGFKAERECYQIDANWT-----EPQNELLNHLRELGVIFIRKRKDGVFFLTQLLTHLAT-NETID-----DVSAEKV--SNGKVIVETNFRVYAYTSSLLQLAIIALFTEMTYRFQDMSVGMITRESVRGALQHGITAAQIISFLRANAHPQCIATSGPVNCLPITVADQIRLWEDERRRMNLKDAYIYSHFESEDEFHGVCEYARQQNILLWSDNQQKLVIVNEDGHELVRQWYKREKS 466

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Celegans
Match: Y73F8A.24 (General transcription factor IIH subunit 4 [Source:UniProtKB/TrEMBL;Acc:D3NQB3])

HSP 1 Score: 160.999 bits (406), Expect = 1.386e-42
Identity = 130/476 (27.31%), Postives = 236/476 (49.58%), Query Frame = 3

Query:  483 LLLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDL-IIFTRVSNGPQGECMRLNKKFQENLKYA--LFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVA-------GIIHKTXXXXXXXXXXXXAGYIKNDEITNEGFQFLLLQKKKQVWQFLISYLEY-IKEPSSIIDILPLLFQI----------------GCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAMKEKS----VVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKN 1817
            L L+F+ T+  +  +++   P     I+R L  +++    ++++      K    +  + + +  +   ++ L DL ++  + ++G     + ++  ++ +  YA  L   +  +LV E NE N +    DV  KKA+++W+ +L  +A         + +T+ N   ++   + G  +  EIT  GFQFLLL   KQ+W ++I YL+  + +   I++++  L QI                 C  ID  +T     + Q E+L H  E G I         F +T LL  L + N+ +      D SA  +  S G +I+E++  +YA+T S LQ+A++ +F ++  RF   +   IT +S+  AL  GIT+ QIISFL  N HP     S     +PIT+ D+I+L E  R R      Y+Y +F ++ EF  +  +A  +N + W + ++ +V+V ED H ++R+++K  K+
Sbjct:   22 LFLDFLITIPPKTRQRLLQKPSCAFFIYRMLPPIAQQTTIQLIW------KGSFPTASDVEETKEIEDQVKLLKDLGLVHRQTTDGK----LNIDTDYKRSYMYAAMLGAAQISSLVLEPNEGNRR--GKDV-EKKAVERWDCILRYLALPSEENTQAVSETTRNLFKKANFTSGGDTQ-IEITTFGFQFLLLSPVKQMWTYVIEYLKLEMSQGQDIVEVIEPLIQIVLLANRVQVAGFKAERECYQIDANWT-----EPQNELLNHLRELGVIFIRKRKDGVFFLTQLLTHLAT-NETID-----DVSAEKV--SNGKVIVETNFRVYAYTSSLLQLAIIALFTEMTYRFQDMSVGMITRESVRGALQHGITAAQIISFLRANAHPQCIATSGPVNCLPITVADQIRLWEDERRRMNLKDAYIYSHFESEDEFHGVCEYARQQNILLWSDNQQKLVIVNEDGHELVRQWYKREKS 470

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Celegans
Match: Y73F8A.24 (General transcription factor IIH subunit 4 [Source:UniProtKB/TrEMBL;Acc:D3NQB3])

HSP 1 Score: 157.918 bits (398), Expect = 6.517e-42
Identity = 124/409 (30.32%), Postives = 208/409 (50.86%), Query Frame = 3

Query:  681 TKSLEE----LNDLIIFTRVSNGPQGECMRLNKKFQENLKYA--LFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVA-------GIIHKTXXXXXXXXXXXXAGYIKNDEITNEGFQFLLLQKKKQVWQFLISYLEY-IKEPSSIIDILPLLFQI------------GCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAMKEKS----VVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKN 1817
            TK +E+    L DL +  R +   +   + ++  ++ +  YA  L   +  +LV E NE N +    DV  KKA+++W+ +L  +A         + +T+ N   ++   + G  +  EIT  GFQFLLL   KQ+W ++I YL+  + +   I++++  L QI             C  ID  +T     + Q E+L H  E G I         F +T LL  L + N+ +      D SA  +  S G +I+E++  +YA+T S LQ+A++ +F ++  RF   +   IT +S+  AL  GIT+ QIISFL  N HP     S     +PIT+ D+I+L E  R R      Y+Y +F ++ EF  +  +A  +N + W + ++ +V+V ED H ++R+++K  K+
Sbjct:   28 TKEIEDQVKLLKDLGLVHRQTTDGK---LNIDTDYKRSYMYAAMLGAAQISSLVLEPNEGNRR--GKDV-EKKAVERWDCILRYLALPSEENTQAVSETTRNLFKKANFTSGGDTQ-IEITTFGFQFLLLSPVKQMWTYVIEYLKLEMSQGQDIVEVIEPLIQIVLLANRGFKAERECYQIDANWT-----EPQNELLNHLRELGVIFIRKRKDGVFFLTQLLTHLAT-NETID-----DVSAEKV--SNGKVIVETNFRVYAYTSSLLQLAIIALFTEMTYRFQDMSVGMITRESVRGALQHGITAAQIISFLRANAHPQCIATSGPVNCLPITVADQIRLWEDERRRMNLKDAYIYSHFESEDEFHGVCEYARQQNILLWSDNQQKLVIVNEDGHELVRQWYKREKS 416

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Celegans
Match: Y73F8A.24 (General transcription factor IIH subunit 4 [Source:UniProtKB/TrEMBL;Acc:D3NQB3])

HSP 1 Score: 156.377 bits (394), Expect = 2.547e-41
Identity = 124/414 (29.95%), Postives = 210/414 (50.72%), Query Frame = 3

Query:  681 TKSLEE----LNDL-IIFTRVSNGPQGECMRLNKKFQENLKYA--LFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVA-------GIIHKTXXXXXXXXXXXXAGYIKNDEITNEGFQFLLLQKKKQVWQFLISYLEY-IKEPSSIIDILPLLFQI----------------GCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAMKEKS----VVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKN 1817
            TK +E+    L DL ++  + ++G     + ++  ++ +  YA  L   +  +LV E NE N +    DV  KKA+++W+ +L  +A         + +T+ N   ++   + G  +  EIT  GFQFLLL   KQ+W ++I YL+  + +   I++++  L QI                 C  ID  +T     + Q E+L H  E G I         F +T LL  L + N+ +      D SA  +  S G +I+E++  +YA+T S LQ+A++ +F ++  RF   +   IT +S+  AL  GIT+ QIISFL  N HP     S     +PIT+ D+I+L E  R R      Y+Y +F ++ EF  +  +A  +N + W + ++ +V+V ED H ++R+++K  K+
Sbjct:   28 TKEIEDQVKLLKDLGLVHRQTTDGK----LNIDTDYKRSYMYAAMLGAAQISSLVLEPNEGNRR--GKDV-EKKAVERWDCILRYLALPSEENTQAVSETTRNLFKKANFTSGGDTQ-IEITTFGFQFLLLSPVKQMWTYVIEYLKLEMSQGQDIVEVIEPLIQIVLLANRVQVAGFKAERECYQIDANWT-----EPQNELLNHLRELGVIFIRKRKDGVFFLTQLLTHLAT-NETID-----DVSAEKV--SNGKVIVETNFRVYAYTSSLLQLAIIALFTEMTYRFQDMSVGMITRESVRGALQHGITAAQIISFLRANAHPQCIATSGPVNCLPITVADQIRLWEDERRRMNLKDAYIYSHFESEDEFHGVCEYARQQNILLWSDNQQKLVIVNEDGHELVRQWYKREKS 420

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Fly
Match: mrn (gene:FBgn0261109 transcript:FBtr0075595)

HSP 1 Score: 214.157 bits (544), Expect = 1.371e-61
Identity = 141/476 (29.62%), Postives = 243/476 (51.05%), Query Frame = 3

Query:  468 NFEKSLLLNFIKTLDS-QDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQG-ECMRLNKKFQENLKYALFGGE-----TCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXX-----AGYIKNDE-----ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAMK-------EKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNS 1820
            N E      +++T  + + +EK++  P  C  +FREL  +++  + R+LF+D+ V ++++ SW  +  +    ++   L  L ++ RV+  P G     L+  F+++++  L GG      T TL K+   S  + IA   L   A+ +W  VL  + G  ++   +    S         A  +K DE     IT +GFQFLLL  + QVW F++ YL+  +E   S+ + L +LFQ+   ++ + Y+           LQH  EFG +         F+ T L +++ S     +     D+ A+      GYI++E++  +YA+TDS LQ+AVL +F +L  RFP      +T  S+ +AL  GIT++QI+S+L    HP M+        KS +P T+VD+IKL E  RNR     G +Y  F + ++F TL ++A+  + + W+N     ++V ++ H  ++++WK    S
Sbjct:   31 NLECKDFQEYLRTRQTPESLEKLYNYPPICLAVFRELPEIARQFIIRILFVDQPVPQAVVTSWGAQRCAKEQAEATSCLTALNVW-RVTAIPGGLSAWELSPTFKKSVRQVLLGGGKPWPMTNTLDKD---SKPRDIA--FLDTYAMSRWRCVLHYMVGTGNRNGTDAEAISPDAVRILLHANLMKRDERDGITITRQGFQFLLLDTRAQVWHFMLQYLDTCEERGISLPECLSMLFQLSFSTLGRDYSSEGMNSQMLTFLQHLREFGLVFQRKRKEGRFYPTRLALNVTSKEAAATASVAMDEEATQ---DCGYIVVETNYRVYAYTDSPLQVAVLGLFTELLYRFPNLVVGVLTRDSVRQALRGGITAEQIVSYLEQYAHPNMRMVESAIHSKSCLPPTVVDQIKLWELERNRFTYTEGVLYNQFLSHTDFVTLRDYAQSIHMLVWQNERTRTMVVQKNGHDDVKRYWKKYSKS 497

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Zebrafish
Match: gtf2h4 (general transcription factor IIH, polypeptide 4 [Source:ZFIN;Acc:ZDB-GENE-030131-6779])

HSP 1 Score: 217.238 bits (552), Expect = 6.112e-63
Identity = 135/465 (29.03%), Postives = 248/465 (53.33%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIF--TRVSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE-----ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSL------GYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKN 1817
            L  ++K L  + +++++  P TC  ++REL  L+K+ V R+LF+D  + ++ +A WV KD      + +  L  L ++    +  G QG    LN  F++NL+ AL GG      E   L  + +  + +S     L + A+++WE +L  + G     + +  +  +++ AG +K++      IT+ GFQFLLL    Q+W F + YL+  +     +++IL  LFQ+   ++ + Y+    +++    LQH  EFG +         ++ T L + L +G            SASS + ++      G+I++E++  IYA+T+S+LQIA++ +F ++  RFP      +T +S+ +A+  GIT+ QII FL    HP M K+  V+P T+ D+I+L E  ++R +   G +Y  F +Q++F+ L + A+    + W+N    +++V    H  +++FWK +K+
Sbjct:   12 LHEYLKELTPEILDRLYNHPATCLAVYRELPPLAKNYVMRMLFLDHPLPQAAVALWVKKDSQKDHDQCVSVLTGLRLWHSQHLQGGLQG--FVLNPVFKDNLRIALLGGGKPWADEGANLGPDRHARDVES-----LDRYAMERWEVILHFMVGS-PSAAVSQDLAQLLIQAGLMKSETGEAPCITSAGFQFLLLDTASQLWYFTLQYLKTAQSRGMDLVEILSFLFQLSFSTLGRDYSVEGMSESLLTFLQHLREFGLVFQRKRKSRRYYPTRLAITLAAG----VTANPASGSASSALGAIPGTGDTGFIVVETNYRIYAYTNSELQIALVALFSEMLYRFPNLVVAQVTRESVQQAISNGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELEKDRLQFTEGVLYNQFLSQADFEVLRDRAQGLGVLVWQNPAHRVMVVTPHGHSEVKRFWKRQKS 464

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Zebrafish
Match: gtf2h4 (general transcription factor IIH, polypeptide 4 [Source:ZFIN;Acc:ZDB-GENE-030131-6779])

HSP 1 Score: 217.238 bits (552), Expect = 9.878e-63
Identity = 135/465 (29.03%), Postives = 248/465 (53.33%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIF--TRVSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE-----ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSL------GYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKN 1817
            L  ++K L  + +++++  P TC  ++REL  L+K+ V R+LF+D  + ++ +A WV KD      + +  L  L ++    +  G QG    LN  F++NL+ AL GG      E   L  + +  + +S     L + A+++WE +L  + G     + +  +  +++ AG +K++      IT+ GFQFLLL    Q+W F + YL+  +     +++IL  LFQ+   ++ + Y+    +++    LQH  EFG +         ++ T L + L +G            SASS + ++      G+I++E++  IYA+T+S+LQIA++ +F ++  RFP      +T +S+ +A+  GIT+ QII FL    HP M K+  V+P T+ D+I+L E  ++R +   G +Y  F +Q++F+ L + A+    + W+N    +++V    H  +++FWK +K+
Sbjct:   30 LHEYLKELTPEILDRLYNHPATCLAVYRELPPLAKNYVMRMLFLDHPLPQAAVALWVKKDSQKDHDQCVSVLTGLRLWHSQHLQGGLQG--FVLNPVFKDNLRIALLGGGKPWADEGANLGPDRHARDVES-----LDRYAMERWEVILHFMVGS-PSAAVSQDLAQLLIQAGLMKSETGEAPCITSAGFQFLLLDTASQLWYFTLQYLKTAQSRGMDLVEILSFLFQLSFSTLGRDYSVEGMSESLLTFLQHLREFGLVFQRKRKSRRYYPTRLAITLAAGVT----ANPASGSASSALGAIPGTGDTGFIVVETNYRIYAYTNSELQIALVALFSEMLYRFPNLVVAQVTRESVQQAISNGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELEKDRLQFTEGVLYNQFLSQADFEVLRDRAQGLGVLVWQNPAHRVMVVTPHGHSEVKRFWKRQKS 482

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Xenopus
Match: gtf2h4 (Xenopus tropicalis general transcription factor IIH subunit 4 (gtf2h4), mRNA. [Source:RefSeq mRNA;Acc:NM_001015845])

HSP 1 Score: 218.779 bits (556), Expect = 1.653e-63
Identity = 138/462 (29.87%), Postives = 251/462 (54.33%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIF--TRVSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE-----ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPSSI-IDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISS--LGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNS 1820
            L  F++ L    +++++  P TC  +FREL  L+K+ V R+LF+++ + ++ +A WV K+      ++ + L  L ++   ++  G QG  + LN  F+ENLK AL GG      + C L  + +  +  S     L K A ++WE +L  + G     + +  +  +++ AG +K++      I++ GFQFLLL    Q+W F++ YL+  +    I ++IL  +FQ+   ++ + Y+    + +    LQH  EFG +         ++ T L ++L SG             + S++ S   G+I++E++  IYA+TDS+LQIA++ +F ++  RFP      +T +++ +A+  GIT++QII FL    HP M ++  V+P T+ D+I+L E  R+R R   G +Y  F +Q +F+ L N+A     + ++N  K +++V    H  +++FWK +K+S
Sbjct:   13 LHEFLQELSPGVLDQLYNHPATCLAVFRELPGLAKNYVMRMLFLEQPLPQAAVALWVKKENFKEHEENTQVLTGLRLWHTQQLPGGLQG--LILNPIFRENLKIALLGGGKPWADDGCLLGPDKHGKDVVS-----LDKYAEERWEVILHFMVGS-PSAAVSQDLAQLLIQAGLMKSESGEAPCISSAGFQFLLLDTPSQLWYFMLQYLKSAESRGMILVEILSFMFQLSFSTLGKDYSVEGMSDSLLTFLQHLREFGLVFQRKRKSRRYYPTRLAINLASG------------ISGSVVDSHKQGFIVVETNYRIYAYTDSELQIALIALFSEMLYRFPNLVVAQVTRENVQQAIGNGITAEQIIHFLRTRAHPVMLQQNPVLPPTITDQIRLWELERDRLRFSEGVLYNQFLSQVDFELLRNYARDLGVLVFENPAKRVMVVTPGGHSDVKRFWKRQKHS 454

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Xenopus
Match: gtf2h4 (Xenopus tropicalis general transcription factor IIH subunit 4 (gtf2h4), mRNA. [Source:RefSeq mRNA;Acc:NM_001015845])

HSP 1 Score: 218.779 bits (556), Expect = 1.746e-63
Identity = 138/462 (29.87%), Postives = 251/462 (54.33%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIF--TRVSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE-----ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPSSI-IDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISS--LGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNS 1820
            L  F++ L    +++++  P TC  +FREL  L+K+ V R+LF+++ + ++ +A WV K+      ++ + L  L ++   ++  G QG  + LN  F+ENLK AL GG      + C L  + +  +  S     L K A ++WE +L  + G     + +  +  +++ AG +K++      I++ GFQFLLL    Q+W F++ YL+  +    I ++IL  +FQ+   ++ + Y+    + +    LQH  EFG +         ++ T L ++L SG             + S++ S   G+I++E++  IYA+TDS+LQIA++ +F ++  RFP      +T +++ +A+  GIT++QII FL    HP M ++  V+P T+ D+I+L E  R+R R   G +Y  F +Q +F+ L N+A     + ++N  K +++V    H  +++FWK +K+S
Sbjct:   19 LHEFLQELSPGVLDQLYNHPATCLAVFRELPGLAKNYVMRMLFLEQPLPQAAVALWVKKENFKEHEENTQVLTGLRLWHTQQLPGGLQG--LILNPIFRENLKIALLGGGKPWADDGCLLGPDKHGKDVVS-----LDKYAEERWEVILHFMVGS-PSAAVSQDLAQLLIQAGLMKSESGEAPCISSAGFQFLLLDTPSQLWYFMLQYLKSAESRGMILVEILSFMFQLSFSTLGKDYSVEGMSDSLLTFLQHLREFGLVFQRKRKSRRYYPTRLAINLASG------------ISGSVVDSHKQGFIVVETNYRIYAYTDSELQIALIALFSEMLYRFPNLVVAQVTRENVQQAIGNGITAEQIIHFLRTRAHPVMLQQNPVLPPTITDQIRLWELERDRLRFSEGVLYNQFLSQVDFELLRNYARDLGVLVFENPAKRVMVVTPGGHSDVKRFWKRQKHS 460

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Xenopus
Match: gtf2h4 (Xenopus tropicalis general transcription factor IIH subunit 4 (gtf2h4), mRNA. [Source:RefSeq mRNA;Acc:NM_001015845])

HSP 1 Score: 168.703 bits (426), Expect = 5.246e-46
Identity = 99/326 (30.37%), Postives = 180/326 (55.21%), Query Frame = 3

Query:  870 LTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE-----ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPSSI-IDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISS--LGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNS 1820
            L K A ++WE +L  + G     + +  +  +++ AG +K++      I++ GFQFLLL    Q+W F++ YL+  +    I ++IL  +FQ+   ++ + Y+    + +    LQH  EFG +         ++ T L ++L SG             + S++ S   G+I++E++  IYA+TDS+LQIA++ +F ++  RFP      +T +++ +A+  GIT++QII FL    HP M ++  V+P T+ D+I+L E  R+R R   G +Y  F +Q +F+ L N+A     + ++N  K +++V    H  +++FWK +K+S
Sbjct:   58 LDKYAEERWEVILHFMVGS-PSAAVSQDLAQLLIQAGLMKSESGEAPCISSAGFQFLLLDTPSQLWYFMLQYLKSAESRGMILVEILSFMFQLSFSTLGKDYSVEGMSDSLLTFLQHLREFGLVFQRKRKSRRYYPTRLAINLASG------------ISGSVVDSHKQGFIVVETNYRIYAYTDSELQIALIALFSEMLYRFPNLVVAQVTRENVQQAIGNGITAEQIIHFLRTRAHPVMLQQNPVLPPTITDQIRLWELERDRLRFSEGVLYNQFLSQVDFELLRNYARDLGVLVFENPAKRVMVVTPGGHSDVKRFWKRQKHS 370

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Mouse
Match: Gtf2h4 (general transcription factor II H, polypeptide 4 [Source:MGI Symbol;Acc:MGI:1338799])

HSP 1 Score: 225.328 bits (573), Expect = 4.751e-66
Identity = 140/462 (30.30%), Postives = 246/462 (53.25%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTR--VSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSD 1823
            L  F+  L    +++++  P TC  +FREL +L+K+ V R+LF+++ + ++ +A WV K++S    +S   L+ L I+    +  G QG  + LN  F++NL+ AL GG      +T  L  + +  +  S     L K A ++WE VL  + G     + +  +  ++  AG +K+ E      IT+ GFQFLLL    Q+W F++ YL+  +     +++IL  LFQ+   ++ + Y+    + +    LQH  EFG +         ++ T L ++L SG             A   +   G+I++E++  +YA+T+S+LQIA++ +F ++  RFP      +T +S+ +A+  GIT+ QII FL    HP M K+  V+P T+ D+I+L E  R+R R   G +Y  F +Q +F+ L+  A     + ++N  K +++V    H  +++FWK +K+S 
Sbjct:   20 LQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQG--LILNPVFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPS-----LDKYAEERWEVVLHFMVGS-PSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVS----------GAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQNPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS 463

[Close]

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTR--VSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSD 1823
            L  F+  L    +++++  P TC  +FREL +L+K+ V R+LF+++ + ++ +A WV K++S    +S   L+ L I+    +  G QG  + LN  F++NL+ AL GG      +T  L  + +  +  S     L K A ++WE VL  + G     + +  +  ++  AG +K+ E      IT+ GFQFLLL    Q+W F++ YL+  +     +++IL  LFQ+   ++ + Y+    + +    LQH  EFG +         ++ T L ++L SG             A   +   G+I++E++  +YA+T+S+LQIA++ +F ++  RFP      +T +S+ +A+  GIT+ QII FL    HP M K+  V+P T+ D+I+L E  R+R R   G +Y  F +Q +F+ L+  A     + ++N  K +++V    H  +++FWK +K+S 
Sbjct:   20 LQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQG--LILNPVFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPS-----LDKYAEERWEVVLHFMVGS-PSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVS----------GAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQNPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS 463

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Mouse
Match: Gtf2h4 (general transcription factor II H, polypeptide 4 [Source:MGI Symbol;Acc:MGI:1338799])

HSP 1 Score: 66.6254 bits (161), Expect = 2.029e-12
Identity = 47/152 (30.92%), Postives = 82/152 (53.95%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTR--VSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLV 917
            L  F+  L    +++++  P TC  +FREL +L+K+ V R+LF+++ + ++ +A WV K++S    +S   L+ L I+    +  G QG  + LN  F++NL+ AL GG      +T  L  + +  +  S     L K A ++WE VL  +
Sbjct:   20 LQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQG--LILNPVFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPS-----LDKYAEERWEVVLHFM 164

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Mouse
Match: Gtf2h4 (general transcription factor II H, polypeptide 4 [Source:MGI Symbol;Acc:MGI:1338799])

HSP 1 Score: 66.2402 bits (160), Expect = 4.318e-12
Identity = 36/110 (32.73%), Postives = 64/110 (58.18%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTR--VSNGPQGECMRLNKKFQENLKYALFGGE 809
            L  F+  L    +++++  P TC  +FREL +L+K+ V R+LF+++ + ++ +A WV K++S    +S   L+ L I+    +  G QG  + LN  F++NL+ AL G  
Sbjct:   20 LQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQG--LILNPVFRQNLRIALLGAR 127

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Mouse
Match: Gtf2h4 (general transcription factor II H, polypeptide 4 [Source:MGI Symbol;Acc:MGI:1338799])

HSP 1 Score: 50.8322 bits (120), Expect = 1.357e-7
Identity = 25/76 (32.89%), Postives = 47/76 (61.84%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLI 713
            L  F+  L    +++++  P TC  +FREL +L+K+ V R+LF+++ + ++ +A WV K++S    K+ EE   L+
Sbjct:   20 LQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFS----KAQEESTGLL 91

[Close]

BLAST of General transcription factor IIH subunit 4 vs. UniProt/SwissProt
Match: sp|O70422|TF2H4_MOUSE (General transcription factor IIH subunit 4 OS=Mus musculus OX=10090 GN=Gtf2h4 PE=1 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 3.323e-65
Identity = 140/462 (30.30%), Postives = 246/462 (53.25%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTR--VSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSD 1823
            L  F+  L    +++++  P TC  +FREL +L+K+ V R+LF+++ + ++ +A WV K++S    +S   L+ L I+    +  G QG  + LN  F++NL+ AL GG      +T  L  + +  +  S     L K A ++WE VL  + G     + +  +  ++  AG +K+ E      IT+ GFQFLLL    Q+W F++ YL+  +     +++IL  LFQ+   ++ + Y+    + +    LQH  EFG +         ++ T L ++L SG             A   +   G+I++E++  +YA+T+S+LQIA++ +F ++  RFP      +T +S+ +A+  GIT+ QII FL    HP M K+  V+P T+ D+I+L E  R+R R   G +Y  F +Q +F+ L+  A     + ++N  K +++V    H  +++FWK +K+S 
Sbjct:   20 LQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQG--LILNPVFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPS-----LDKYAEERWEVVLHFMVGS-PSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVS----------GAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQNPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS 463

[Close]

BLAST of General transcription factor IIH subunit 4 vs. UniProt/SwissProt
Match: sp|P60027|TF2H4_PANTR (General transcription factor IIH subunit 4 OS=Pan troglodytes OX=9598 GN=GTF2H4 PE=3 SV=1)

HSP 1 Score: 224.942 bits (572), Expect = 4.423e-65
Identity = 140/462 (30.30%), Postives = 246/462 (53.25%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTR--VSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSD 1823
            L  F+  L    +++++  P TC  +FREL +L+K+ V R+LF+++ + ++ +A WV K++S    +S   L+ L I+    +  G QG  + LN  F++NL+ AL GG      +T  L  + +  +  S     L K A ++WE VL  + G     + +  +  ++  AG +K+ E      IT+ GFQFLLL    Q+W F++ YL+  +     +++IL  LFQ+   ++ + Y+    + +    LQH  EFG +         ++ T L ++L SG             A   +   G+I++E++  +YA+T+S+LQIA++ +F ++  RFP      +T +S+ +A+  GIT+ QII FL    HP M K+  V+P T+ D+I+L E  R+R R   G +Y  F +Q +F+ L+  A     + ++N  K +++V    H  +++FWK +K+S 
Sbjct:   19 LQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQG--LILNPIFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPS-----LDKYAEERWEVVLHFMVGS-PSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVS----------GAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS 462

[Close]

BLAST of General transcription factor IIH subunit 4 vs. UniProt/SwissProt
Match: sp|Q92759|TF2H4_HUMAN (General transcription factor IIH subunit 4 OS=Homo sapiens OX=9606 GN=GTF2H4 PE=1 SV=1)

HSP 1 Score: 224.942 bits (572), Expect = 4.423e-65
Identity = 140/462 (30.30%), Postives = 246/462 (53.25%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTR--VSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSD 1823
            L  F+  L    +++++  P TC  +FREL +L+K+ V R+LF+++ + ++ +A WV K++S    +S   L+ L I+    +  G QG  + LN  F++NL+ AL GG      +T  L  + +  +  S     L K A ++WE VL  + G     + +  +  ++  AG +K+ E      IT+ GFQFLLL    Q+W F++ YL+  +     +++IL  LFQ+   ++ + Y+    + +    LQH  EFG +         ++ T L ++L SG             A   +   G+I++E++  +YA+T+S+LQIA++ +F ++  RFP      +T +S+ +A+  GIT+ QII FL    HP M K+  V+P T+ D+I+L E  R+R R   G +Y  F +Q +F+ L+  A     + ++N  K +++V    H  +++FWK +K+S 
Sbjct:   19 LQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQG--LILNPIFRQNLRIALLGGGKAWSDDTSQLGPDKHARDVPS-----LDKYAEERWEVVLHFMVGS-PSAAVSQDLAQLLSQAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLGKDYSVEGMSDSLLNFLQHLREFGLVFQRKRKSRRYYPTRLAINLSSGVS----------GAGGTVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHARELGVLVFENSAKRLMVVTPAGHSDVKRFWKRQKHSS 462

[Close]

BLAST of General transcription factor IIH subunit 4 vs. UniProt/SwissProt
Match: sp|Q6CLR2|TFB2_KLULA (General transcription and DNA repair factor IIH subunit TFB2 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=TFB2 PE=3 SV=1)

HSP 1 Score: 193.356 bits (490), Expect = 5.408e-53
Identity = 129/495 (26.06%), Postives = 238/495 (48.08%), Query Frame = 3

Query:  468 NFEKSLLLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECMRLNKKFQENLKYALFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE-----ITNEGFQFLLLQKKKQVWQFLISYLEYIKE-PSSIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLS------------------GNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAMKEKS-------------------VVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSD 1823
            N  K+ + ++++ L  Q   +++ SP TC  I+R LS ++K  +  +LF D  V    +  WV  D  Y L  S++ +  L +        Q   +RLN  F+++ K  L GGE      +  + +   +++  L + + +KWE++L  + G  +  +    V  ++ ++G ++  E     ITN+GFQFLL     Q+W  L+ YL+  +      +D+L  +F +G   + + Y C   +  QR MLQ   ++G I      +  F+ T L   L S                   N+  +++    Q  ++  +  G +I+E++  +Y++++S LQIA+L +F  L  RF       +T +S+  AL  GIT++QII++L  + HP M+  +                   V+P T+VD+I+L +   +R     GY+Y +F +  E+ T+ ++A+    + W+N +K M  V  + +  +  F  +R+N D
Sbjct:    4 NLFKTTVNDYLEGLPEQVQSRLYESPATCLAIYRLLSPMAKFFIMSMLFQDHDVSLRDLDKWVKPDAKYQLQYSIKSMKSLNLIIE-GESKQPLLIRLNPIFKKSFKNVLTGGEINNSFGDVADDDTNPVSTATLDQYSAEKWETILHYMVGTPNTNTPGGKVLDLLQHSGLMEEAEYGELKITNQGFQFLLQDVNAQMWTLLLQYLKMAESLQMDPVDVLNFIFMLGALQLGKAYKCDQLSNTQRTMLQDMRDYGLIYQNQSDYAKFYPTRLATLLTSDTKAFRSASVALDSVLNKANETTAVEGDSGQDETTERTQDGALIIETNFKLYSYSNSPLQIAILSLFVHLKSRFANMVTGQLTRESVRNALLNGITAEQIIAYLETHAHPRMRRLAEENLSKKLELDPTVKETLQVLPPTVVDQIRLWQLELDRIISYDGYLYTDFESYQEYQTVADYAKDIGVLLWQNEKKKMFFVSTEGNSQVLDF--HRRNFD 495

[Close]

BLAST of General transcription factor IIH subunit 4 vs. UniProt/SwissProt
Match: sp|Q75B51|TFB2_ASHGO (General transcription and DNA repair factor IIH subunit TFB2 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=TFB2 PE=3 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 1.421e-50
Identity = 142/509 (27.90%), Postives = 240/509 (47.15%), Query Frame = 3

Query:  468 NFEKSLLLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECMRLNKKFQENLKYALFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIK----EPSSIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSL--------------------------------GYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAMK-------EK------------SVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNR 1811
            N  K+ +  +++ L      +++ SP TC  I+R LS ++K  +  ++F DE V    +  WV  D  + L  +++ +  L + T   +G Q   ++LN  F+E+ K AL GGE         E     +   +L   A DKWE++L  + G     S    V S++ ++  ++ DE      ITNEGFQFLL     Q+W  L+ YL   +    +P   +D+L L+F IG   + + Y+    ++ Q+ MLQ   ++G +  +      F+ T L   L S  D +S++     SAS  ++S+                                G +I+E++  +Y++++S LQIA+L +F  L  RF       IT +SI RAL  GIT+DQII+++  + HP M+       EK             V+P T+VD+IKL +   +R     GY++++F N  E+  L  +A     + W + +K M  V ++ +  +  F K +
Sbjct:   13 NLFKNTVNEYLQELPQPVQSRLYQSPATCLAIYRLLSPMAKFFIMSMIFQDEEVSLRDLDRWVKPDAKFQLHDAIKSMKLLHLITEGRSG-QPLMVQLNSIFKESFKNALTGGEVKNSFGNVVEEENDPVTMAMLDSYAADKWETILHFMVGTPLTKSPGKNVLSLLRHSKLMEVDESSKELKITNEGFQFLLQDANAQIWTLLLQYLTMAETFQMDP---VDVLNLIFMIGALELGKAYSVVGLSETQKTMLQDLRDYGLVFQKQSNLSKFYPTRLATMLTS--DVVSIR-----SASGAVNSVLRQRAEGVDGKVLNGTALGDDDLQAGGEGALDGALIVETNFKLYSYSNSPLQIAILSLFIHLKTRFQNMVTGQITRESIRRALHNGITADQIIAYMETHAHPQMRRLAGDNLEKKLELDPNCRDTLQVLPPTVVDQIKLWQLELDRIISYDGYLFRDFDNLQEYQVLAQYARDIGVLLWSDDKKKMFFVSKEGNAQVIDFHKRK 510

[Close]

BLAST of General transcription factor IIH subunit 4 vs. TrEMBL
Match: A0A4Y7NK39 (General transcription factor IIH subunit 4 OS=Moina brachiata OX=675436 GN=EOG090X04KD PE=2 SV=1)

HSP 1 Score: 263.462 bits (672), Expect = 5.095e-77
Identity = 149/448 (33.26%), Postives = 248/448 (55.36%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECMRLNKKFQENLKYALFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPA-MKEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWK 1805
            L +++KTL +  +++++  P TC  +FREL  LS+H + R+LF+D+ V K+++ SW++ + +  L +  + L +L I+  V      E + LN  F+ NLK AL GG     +K   E + KS     L   AI++WE VL  + G     + ++    ++ +AG +K +       IT +GFQFLL+    QVW FL+ YL+     +  +++ L  LFQ+   ++ Q Y+    +++    LQH  EFG +         F+ T L +D+ +G  +  L      S SS+  + GYII+E++  +YA+TDS LQ+A++ +FC+L  RFP      IT +SI +AL  GIT++QI+SFL  + HP   K   ++P T+ D+IKL    R+R     G +Y  F +Q++FD L N+A+ +  + W +     ++V  + H  ++KFWK
Sbjct:   13 LHSYLKTLPAGTLDRLYNHPATCLAVFRELPELSRHYIMRILFVDQAVPKAIMGSWISSNNTKELEELFKLLGELRIWHTVEMAGGLEGLLLNPAFRRNLKGALLGGGNEWSIKPPAEPDPKSRDIGFLDAYAIERWECVLHFMVGSRQHDAISSDALQLLHHAGLMKKENGDSQMTITRDGFQFLLMDTSSQVWYFLLQYLDTATARNLDLVECLGFLFQLSFSTLGQDYSTDNMSESLSRFLQHLREFGLVFQRKRKAGRFYPTRLALDIAAGPKKAMLG-----SMSSLSPTTGYIIVETNYRVYAYTDSTLQVALIALFCELMYRFPNLVVGIITRESIRQALRSGITAEQIVSFLRQHSHPERYKTPPILPPTIADQIKLWSLERDRFTYKDGVLYNQFDSQADFDVLKNYAQERGVLVWHSVTGRKMVVTRNGHDEVKKFWK 455

[Close]

BLAST of General transcription factor IIH subunit 4 vs. TrEMBL
Match: A0A4Y7M1Y0 (General transcription factor IIH subunit 4 OS=Daphnia hispanica OX=575233 GN=EOG090X04KD PE=2 SV=1)

HSP 1 Score: 262.692 bits (670), Expect = 9.014e-77
Identity = 152/449 (33.85%), Postives = 249/449 (55.46%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECMRLNKKFQENLKYALFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKND------EITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHP-AMKEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKN 1808
            L +++KTL +  +++++  P TC  +FREL  LS+  + R+LF+D+ V K+++ SWV+ + +  L   ++ L+DL ++  V      +   LN  F+ NLK AL GG     +K   +++ K+    +L + A+ +WE VL  + G       ++    ++ +AG +K +       IT +GFQFLL+    QVW FL+ YL+        +ID L  LFQ+   ++ Q Y+  + +   ++ LQH  EFG +         F+ T L +D+ SG  +  L   R   A+S   S GYII+E++  +YA+TDS LQ+A++ +FC+L  RFP      IT +SI +AL  GIT+DQI+SFL  + HP   KE  V+P T+ D+IKL    R+R     G +Y  F +Q++F+ L N+A+ +  + W       ++V ++ H  +RKFWK 
Sbjct:   13 LHSYLKTLPATVLDRLYNHPATCLAVFRELPELSRLYIMRILFVDQAVPKAIMGSWVSPNSAKELEDIVKLLSDLRLWQEVEMQGGLKGWLLNPTFRRNLKGALLGGGNEWSMKPPTDTDPKARGITILDEYAMGRWECVLHFMVGSHQHEVISSDALQILQHAGLMKKEVGENQLTITRDGFQFLLMDTSAQVWYFLLQYLDTATARGLDLIDCLGFLFQLSFSTLGQDYSTDSMSDGLQKFLQHLREFGLVYQRKRKDGRFYPTRLALDIASGPKKSMLNTMR---ATSSNQSSGYIIVETNYRVYAYTDSSLQVALIALFCELMYRFPNLVVGIITRESIRQALRSGITADQIVSFLRQHAHPECYKEPPVLPSTIADQIKLWAIERDRFIYKDGVLYNQFDSQADFEILKNYAQERGVLVWHTIHGRKMVVTKEGHEEVRKFWKR 458

[Close]

BLAST of General transcription factor IIH subunit 4 vs. TrEMBL
Match: A0A4Y7M1C8 (General transcription factor IIH subunit 4 OS=Daphnia atkinsoni OX=342845 GN=EOG090X04KD PE=2 SV=1)

HSP 1 Score: 260.766 bits (665), Expect = 6.736e-76
Identity = 151/449 (33.63%), Postives = 248/449 (55.23%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECMRLNKKFQENLKYALFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHP-AMKEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKN 1808
            L +++KTL +  +++++  P TC  +FREL  LS+  + R+LF+D+ V K+++ SWV+ + +  L   ++ L +L ++  V      +   LN  F+ NLK AL GG     +K   +++ K+    +L + A+ +WE VL  + G       ++    ++ +AG +K +       IT +GFQFLL+    QVW FL+ YL+        +ID L  LFQ+   ++ Q Y+    T+  ++ LQH  EFG +         F+ T L +D+ SG  +  L       A+S   S GYII+E++  +YA+TDS LQ+A++ +FC+L  RFP      IT +SI +AL  GIT+DQI+SFL  + HP   KE  V+P T+ D+IKL    R+R     G +Y  F +Q++F+ L N+A+ +  + W   +   ++V ++ H  +RKFWK 
Sbjct:   22 LHSYLKTLPATVLDRLYNHPATCLAVFRELPELSRLYIMRILFVDQAVPKAIMGSWVSPNSAKELEDIVKLLTELRLWQEVEMQGGLKGWLLNPTFRRNLKGALLGGGNDWSMKPPTDTDPKARGIAILDEYAMGRWECVLHFMVGSHQHEVISSDALQILQHAGLMKKEPGENQLTITRDGFQFLLMDTSAQVWYFLLQYLDTATARGLDLIDCLGFLFQLSFSTLGQDYSTDLMTEGLQKFLQHLREFGLVYQRKRKDGRFYPTRLALDIASGPKKSMLNT---MKATSSNQSSGYIIVETNYRVYAYTDSSLQVALIALFCELMYRFPNLVVGIITRESIRQALRSGITADQIVSFLRQHAHPECYKEPPVLPSTIADQIKLWAIERDRFIYKDGVLYNQFDSQADFEILKNYAQERGVLVWHTIQGRKMVVTKEGHEEVRKFWKR 467

[Close]

BLAST of General transcription factor IIH subunit 4 vs. TrEMBL
Match: A0A0R3UG68 (General transcription factor IIH subunit 4 OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS6190 PE=3 SV=1)

HSP 1 Score: 260.381 bits (664), Expect = 1.247e-75
Identity = 150/481 (31.19%), Postives = 270/481 (56.13%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECMRLNKKFQENLKYALFGGETCTLVKEYNESNFKSIA--SDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKND------EITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLL----------------------------SGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRK 1814
            + +++KTLD+ D+++++  P TC ++FREL  LSKH++ R+LF+++T+ KS+++ WV+K    +L ++  +L+DL ++  + +       RLNKKFQE++K +LFGG    L+ ++  +  +     +D L   A ++W+S+L  + G          VR V++ +G +K         IT  GFQFLL+ +  QV +F++ Y +Y+KE   ++I+ L  +FQ+   SI + Y  +  + A++E+LQH  E G           F+VT L + L                             +G  + ++K G  +S  +  + +G+I++E++  +YA+TDS L+ A+L +F K+  R+P      IT  S+  AL +GI++DQIISFLM+N HP M ++  ++P TLVD+I+L E  R R     G +Y+ F   ++F+ + ++A+    + W+  E+ +++V +  H  +R FWK ++
Sbjct:   10 IFDYLKTLDANDLDELYGHPPTCLIVFRELRELSKHLILRLLFLEQTIPKSIVSGWVSKGSQELLNQACTDLSDLRVWHSIDSNSARGSWRLNKKFQESMKVSLFGGGN-QLLSDFGSTTAEKHPKDADFLENYASERWDSILHFMVG-SDNAEVGTCVRDVLLLSGLMKRGGPDFSMGITQRGFQFLLMDRSSQVLRFMLHYFDYLKESRMNLIESLQFVFQLSFLSIGKSYPISMLSTAKQEILQHMRELGLAYQRKRTAPRFYVTRLALSLAFGTSTISSSVRSSLDGEHSLLFSTNPLPTGVSQRAVKAGGTESGGA--ADVGFILVETNFRLYAYTDSPLRTALLSLFSKIRVRYPNMVVADITRDSVREALLRGISADQIISFLMSNAHPDMLRQPEILPPTLVDQIRLWELERERLFFQEGCLYEQFAKNTDFEMIRDYAKGHGYLLWECPERRLMVVSKAGHDDVRAFWKQKR 486

[Close]

BLAST of General transcription factor IIH subunit 4 vs. TrEMBL
Match: A0A164RYY7 (General transcription factor IIH subunit 4 OS=Daphnia magna OX=35525 GN=APZ42_026818 PE=3 SV=1)

HSP 1 Score: 258.84 bits (660), Expect = 3.287e-75
Identity = 147/448 (32.81%), Postives = 248/448 (55.36%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECMRLNKKFQENLKYALFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE------ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHP-AMKEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWK 1805
            L +++KTL +  +++++  P TC  +FREL  LS+  + R+LF+D+ V K+++ SWV+ + +  L   ++ L +L ++  V      +   LN  F+ NLK AL GG     +K   +++ K+    +L + A+ +WE VL  + G       ++    ++ +AG +K +       IT +GFQFLL+    QVW FL+ YL+        +ID L  LFQ+   ++ Q Y+  + +   ++ LQH  EFG +         F+ T L +D+ SG  +  L      +A+S   + GYI++E++  +YA+TDS LQ+A++ +FC+L  RFP      IT +S+ +AL  GIT+DQI+SFL  + HP   KE  V+P T+ D+IKL    R+R     G +Y  F +Q++F+ L N+A+ +  + W       ++V ++ H  +RKFWK
Sbjct:   13 LHSYLKTLTATVLDRLYNHPATCLAVFRELPELSRLYIMRILFVDQAVPKAIMGSWVSPNSAKELEDIVKLLTELRLWQEVEMQGGLKGWLLNPTFRRNLKGALLGGGNEWSMKPPTDADPKARGIAILDEYAMGRWECVLHFMVGSHQHEVISSDALQILQHAGLMKKEPGENQLTITRDGFQFLLMDTSAQVWYFLLQYLDTATARGLDLIDCLGFLFQLSFSTLGQDYSTDSMSDGLQKFLQHLREFGLVYQRKRKDGRFYPTRLALDIASGPKKSMLNA---MTATSSNQNSGYIVVETNYRVYAYTDSSLQVALIALFCELMYRFPNLVVGIITRESVRQALRSGITADQIVSFLRQHAHPECYKEPPVLPSTIADQIKLWAIERDRFVYKDGVLYNQFDSQADFEILKNYAQERGVLVWHTTHGRKMVVTKEGHEEVRKFWK 457

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Cavefish
Match: gtf2h4 (general transcription factor IIH subunit 4 [Source:NCBI gene;Acc:103042913])

HSP 1 Score: 216.468 bits (550), Expect = 8.884e-63
Identity = 133/462 (28.79%), Postives = 247/462 (53.46%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIF--TRVSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE-----ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSG---NDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKN 1817
            L  +++ L    +++++  P TC  ++REL +L+K+ V R+LF+D  + ++ +A WV KD      + +  L  L ++   ++  G QG  + LN  F++NL+ AL GG      E  +L  + +  + +S     L + A+++WE +L  + G     + +  +  +++ AG +K++      IT+ GFQFLLL    Q+W   + YL  ++     +++IL  LFQ+   ++ + Y+    +++ +  LQH  EFG +         ++ T L + L +G   N   S       SA S     G+I++E++  +YA+TDS+LQIA++ +F ++  R        +T +S+ +A+  GIT+ QII FL    HP M K+  V+P T+ D+I+L E  R+R +   G +Y  F +Q++F+ L + A+    + W+N    +++V    H  +++FWK +K+
Sbjct:   12 LHEYLRELSPDILDRLYNHPATCLAVYRELPSLAKNYVMRMLFLDHPLPQAAVALWVKKDSQKDHDQCVSVLTGLRLWHNQQLQGGLQG--IVLNPVFKDNLRIALLGGGKAWADEGSSLGPDRHARDVES-----LDRYAMERWEVILHFMVGS-PSAAVSQDLAQLLIQAGLMKSEAGDAPCITSAGFQFLLLDTASQLWYITLQYLNTVQSRGMDLVEILSFLFQLSFSTLGRDYSVEGMSESLQTFLQHLREFGLVFQRKRKSRRYYPTRLAITLAAGVTANSSSSSSSSTAVSAMSGTGESGFIVVETNYRVYAYTDSELQIALVALFSEMLYRLSNVVVAQVTRESVQQAIANGITAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLQFTEGVLYNQFLSQADFEVLRDRAQGLGVLVWQNAPHRVMVVTPHGHSEVKRFWKRQKS 465

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Sea Lamprey
Match: gtf2h4 (general transcription factor IIH, polypeptide 4 [Source:ZFIN;Acc:ZDB-GENE-030131-6779])

HSP 1 Score: 120.168 bits (300), Expect = 1.402e-31
Identity = 62/194 (31.96%), Postives = 115/194 (59.28%), Query Frame = 3

Query: 1251 FHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHT---DSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNS 1820
            ++ T L ++L SG     + +G     S+ +   GY+++E++  +YA+T   DS+LQ+A++ +FC++  RFP      +T +S+ +A+  GIT++QII FL  + HP M K+  V+P T+ D+I+L E  R+R     G +Y  F +Q++++ L   A+    + W+   + +++V    H  +++FWK +KNS
Sbjct:    7 YYPTRLAINLASG-----ISQG-----STDVHQQGYLVVETNYRVYAYTAVLDSELQVALVALFCEMLYRFPNLVVAQVTRESVQQAIANGITAEQIIHFLRTHAHPEMMKQTPVLPPTITDQIRLWEMERDRLVFTEGVLYSQFLSQADYEVLCVRAKELGVLQWECAARRLMVVTPAGHTDVKRFWKRQKNS 190

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Sea Lamprey
Match: gtf2h4 (general transcription factor IIH, polypeptide 4 [Source:ZFIN;Acc:ZDB-GENE-030131-6779])

HSP 1 Score: 90.5077 bits (223), Expect = 8.739e-21
Identity = 69/232 (29.74%), Postives = 117/232 (50.43%), Query Frame = 3

Query:  567 RELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTR--VSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYI----KNDEITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFI 1223
            RELS L+++ V R+LF++  +  + I SWV KD      +S + L +L ++    +  G  G  + LN  F+ NL+ AL GG      +T  L  + +  +  S     L + A+++WE +L  + G       +  V  +++ AG +    K   IT+ GFQFLLL    QVW F++ YL   +     +++IL  LFQ+   ++ + Y+    +++    LQH  EFG +
Sbjct:    1 RELSPLARNYVMRMLFLETPLPTAAIDSWVCKDAQKAHEESAQLLRELRVWQDHVIPGGLPG--LVLNPVFRANLRTALLGGGNPWSDDTTGLGPDKHARDVTS-----LDRYALERWEVILHFMVGS-PGAEVSADVAQLLIKAGLMRRLKKQISITSAGFQFLLLDTASQVWYFMLQYLNSTETRGMDLVEILSFLFQLSFSTLGKDYSVEGMSESLLNFLQHLREFGMV 224

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Yeast
Match: TFB2 (Subunit of TFIIH and nucleotide excision repair factor 3 complexes; involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH [Source:SGD;Acc:S000006043])

HSP 1 Score: 163.696 bits (413), Expect = 4.898e-44
Identity = 130/500 (26.00%), Postives = 229/500 (45.80%), Query Frame = 3

Query:  495 FIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECM-RLNKKFQENLKYALFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGII------HKTXXXXXXXXXXXXAGYIKNDEITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMS---------LKEGRDQ-----------SASSIISS--------------LGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAMKEKS-------------------VVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNR 1811
            +++ +  Q   +++ SP TC  I+R L  L+K  +  ++F +  V    +  WVN +       +++ +  L +   + N   G  M  LN  F+ +L+ AL GGE         E N  S+  D+L + + +KWE++L  + G         K  N      ++         +ITNEGFQFLL +   Q+W  L+ YL+ I+     ++D+L  +F +G   + + Y     ++ QR MLQ   ++G +  +      F+ T L + L S    +          L++ R++           S + I +S               G +I+E++  IY++++S LQIAVL +F  L  RF       IT +SI RAL  GIT+DQII++L  + HP M+  +                   V+P T+VD+I+L +   +R     G +Y +F    E++ L  +A+    + WK+ +K    + ++ +  +  F K +
Sbjct:   13 YLEEIPQQVQNRLYTSPATCLAIYRILPPLAKFFIMAMVFNENEVPLLDLDKWVNSNGKLQFQNAIKSMKSLHLL--IPNKSSGTLMINLNPTFKISLRNALTGGEVQNSFGVVVEENVVSL--DLLDEYSANKWETILHFMVGTPLAKIPSEKVLNLLKHSKLMEEVNSTGEFKITNEGFQFLLQEINSQLWTLLLQYLKMIETSKMDLVDVLHFIFMLGALEVGKAYKIDALSETQRIMLQDMRDYGLVFQKHSNDSIFYPTKLALMLTSDTKTIRSASNAMDSVLRQNREEPSVNEDGANGKSTTDITTSDDLNKAGLKNQDIPDGSLIVETNFKIYSYSNSPLQIAVLSLFVHLKARFVNMVLGQITRESIRRALTNGITADQIIAYLETHAHPQMRRLAEEKLEKKLELDPNCKEPLQVLPPTVVDQIRLWQLELDRVITYEGSLYSDFETSQEYNLLSKYAQDIGVLLWKDDKKKKFFISKEGNSQVLDFAKRK 508

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Nematostella
Match: EDO38911 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBP9])

HSP 1 Score: 227.639 bits (579), Expect = 2.601e-67
Identity = 142/465 (30.54%), Postives = 242/465 (52.04%), Query Frame = 3

Query:  465 ENFEKSLLLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECMRLNKKFQENLKYALFGGETCTL-VKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGII----HKTXXXXXXXXXXXXAGYIK------NDEITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNS 1820
             + ++  L  ++  L    ++++F  P TC  +FREL  L+KH V R LF D+ V +S++++WV   Y      SL  L  L I+  V +G       +N  F+ N+K AL GG    +   ++   +  +   D L K AI++WESVL  + G      +    +  V  V+V +G +K      N  I+  GFQFLLL +  QVW F++  LE ++     +++ L LLFQ+   S  + Y     T +Q + LQ   E G +         ++ T L V+L +        +G + + S I    G+II+E++  +YA+T+S LQ++++ +FC++  RFP      +T +S  +AL  GI+++QI++FL    HP M K   ++P T+ D+++L E  R+R +   G +Y  F +Q++F+ L  +AE    + W N  K +V+V    H  +++FWK ++  
Sbjct:   15 RHLQRKDLYEYLSGLPVGILDRLFCHPATCLTVFRELPELAKHFVMRTLFADQPVPESIVSTWVKSAYYKYNIGSLNTLKQLRIWREVPSGVHKR-YEMNATFRTNMKAALCGGGKSWMGSTQHLGPDKHTREPDFLDKYAIERWESVLHFMTGSTEMADNAGGVSQDVVKVLVLSGLMKCESPGSNPIISPAGFQFLLLDRPSQVWYFMLQCLETVEARGMDLVECLSLLFQLSFSSPGKDYPTDGLTDSQMKFLQQLREIGLVFQRKRKSRRYYPTKLSVNLTAAG------KGINTTDSQI--EAGFIIIETNYRVYAYTESCLQVSLIGLFCEILCRFPNLCVASLTRESCQQALASGISAEQILNFLQTRAHPEMLKRTPIIPSTISDQVRLWEMERSRMKFTEGVLYNQFLSQADFEMLRKYAEDLGVLIWANSTKRVVVVSRSGHDDVKRFWKRQRQG 470

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Medaka
Match: gtf2h4 (general transcription factor IIH subunit 4 [Source:NCBI gene;Acc:101173874])

HSP 1 Score: 212.231 bits (539), Expect = 3.264e-61
Identity = 130/459 (28.32%), Postives = 244/459 (53.16%), Query Frame = 3

Query:  486 LLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIF--TRVSNGPQGECMRLNKKFQENLKYALFGG------ETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDE-----ITNEGFQFLLLQKKKQVWQFLISYLEYIKEPS-SIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAM-KEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKN 1817
            L  +++ L    +++++  P TC  + REL  L+K+ V R+LF+++ + ++ +A WV KD      + +  L  L ++   ++  G QG    LN  F++NL+ AL GG      E   L  + +  + +S     L + A+++WE +L  + G    ++ +  +  ++V AG +K++      IT+ GFQFLLL    Q+W   + YL   +     +++IL  LFQ+   ++ + Y+    +++    LQH  EFG +         ++ T L + L +G   +S     + + +      G+I++E++  IYA+T+S+LQIA++ +F ++  RFP     H+T +S+ +A+  GIT+ QII FL    HP M K+   +P T+ D+I+L E  R+R +   G +Y  F +Q++F+ L + A+    + W++     ++V    H  ++KFWK +++
Sbjct:   12 LHEYLRELGPDVLDRLYNHPATCLAVCRELPLLAKNYVMRMLFLEQPLPQAAVALWVRKDGQRDHDECVSVLTGLRLWHSQQLQGGLQGYI--LNPVFKDNLRIALLGGGKVWADEGIILGPDRHARDIES-----LDRYAMERWEVILQFMVG--SPSAVSQDLAQLLVQAGLMKSEAGEAPYITSAGFQFLLLDTASQLWYLTLQYLNTAQSRGMELVEILSFLFQLSFSTLGRDYSVEGMSESLLTFLQHLREFGLVFQRKRKSRRYYPTRLAITLAAG---VSSNSPSNMTNTPGTGDAGFIVVETNYRIYAYTNSELQIALVALFSEMLYRFPNVVVAHLTRESVQQAIANGITAQQIIHFLRTRAHPVMLKQSPALPPTITDQIRLWELERDRLQFTEGVLYNQFLSQADFEVLRDRAQGLGCLVWQDASHRAMVVTPQGHSEVKKFWKRQRS 458

[Close]

BLAST of General transcription factor IIH subunit 4 vs. Planmine SMEST
Match: SMESG000022992.1 (SMESG000022992.1)

HSP 1 Score: 917.146 bits (2369), Expect = 0.000e+0
Identity = 456/456 (100.00%), Postives = 456/456 (100.00%), Query Frame = 3

Query:  462 MENFEKSLLLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECMRLNKKFQENLKYALFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTXXXXXXXXXXXXAGYIKNDEITNEGFQFLLLQKKKQVWQFLISYLEYIKEPSSIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAMKEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSDYL 1829
            MENFEKSLLLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECMRLNKKFQENLKYALFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTSNNNIVRSVIVNAGYIKNDEITNEGFQFLLLQKKKQVWQFLISYLEYIKEPSSIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAMKEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSDYL
Sbjct:    1 MENFEKSLLLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVLFIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECMRLNKKFQENLKYALFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLSLVAGIIHKTSNNNIVRSVIVNAGYIKNDEITNEGFQFLLLQKKKQVWQFLISYLEYIKEPSSIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLEFGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYIIMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDKGITSDQIISFLMNNGHPAMKEKSVVPITLVDKIKLVEFNRNRTRPVSGYMYQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNRKNSDYL 456

The following BLAST results are available for this feature:

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Human
BLAST of General transcription factor IIH subunit 4 vs. Ensembl Celegans
BLAST of General transcription factor IIH subunit 4 vs. Ensembl Fly
BLAST of General transcription factor IIH subunit 4 vs. Ensembl Zebrafish
BLAST of General transcription factor IIH subunit 4 vs. Ensembl Xenopus
BLAST of General transcription factor IIH subunit 4 vs. Ensembl Mouse
BLAST of General transcription factor IIH subunit 4 vs. UniProt/SwissProt
BLAST of General transcription factor IIH subunit 4 vs. TrEMBL
BLAST of General transcription factor IIH subunit 4 vs. Ensembl Cavefish
BLAST of General transcription factor IIH subunit 4 vs. Ensembl Sea Lamprey
BLAST of General transcription factor IIH subunit 4 vs. Ensembl Yeast
BLAST of General transcription factor IIH subunit 4 vs. Ensembl Nematostella
BLAST of General transcription factor IIH subunit 4 vs. Ensembl Medaka
BLAST of General transcription factor IIH subunit 4 vs. Planmine SMEST

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
GTF2H4	9.210e-66	30.30	general transcription factor IIH subunit 4 [Source...	[more]
GTF2H4	9.210e-66	30.30	general transcription factor IIH subunit 4 [Source...	[more]
GTF2H4	9.210e-66	30.30	general transcription factor IIH subunit 4 [Source...	[more]
GTF2H4	9.210e-66	30.30	general transcription factor IIH subunit 4 [Source...	[more]
GTF2H4	9.210e-66	30.30	general transcription factor IIH subunit 4 [Source...	[more]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4


Match Name	E-value	Identity	Description
Y73F8A.24	3.693e-43	27.60	General transcription factor IIH subunit 4 [Sourc...	[more]
Y73F8A.24	1.386e-42	27.31	General transcription factor IIH subunit 4 [Sourc...	[more]
Y73F8A.24	6.517e-42	30.32	General transcription factor IIH subunit 4 [Sourc...	[more]
Y73F8A.24	2.547e-41	29.95	General transcription factor IIH subunit 4 [Sourc...	[more]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1


Match Name	E-value	Identity	Description
mrn	1.371e-61	29.62	gene:FBgn0261109 transcript:FBtr0075595	[more]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
gtf2h4	6.112e-63	29.03	general transcription factor IIH, polypeptide 4 [S...	[more]
gtf2h4	9.878e-63	29.03	general transcription factor IIH, polypeptide 4 [S...	[more]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3


Match Name	E-value	Identity	Description
gtf2h4	1.653e-63	29.87	Xenopus tropicalis general transcription factor II...	[more]
gtf2h4	1.746e-63	29.87	Xenopus tropicalis general transcription factor II...	[more]
gtf2h4	5.246e-46	30.37	Xenopus tropicalis general transcription factor II...	[more]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Gtf2h4	4.751e-66	30.30	general transcription factor II H, polypeptide 4 [...	[more]
Gtf2h4	4.751e-66	30.30	general transcription factor II H, polypeptide 4 [...	[more]
Gtf2h4	2.029e-12	30.92	general transcription factor II H, polypeptide 4 [...	[more]
Gtf2h4	4.318e-12	32.73	general transcription factor II H, polypeptide 4 [...	[more]
Gtf2h4	1.357e-7	32.89	general transcription factor II H, polypeptide 4 [...	[more]

BLAST of General transcription factor IIH subunit 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|O70422\|TF2H4_MOUSE	3.323e-65	30.30	General transcription factor IIH subunit 4 OS=Mus ...	[more]
sp\|P60027\|TF2H4_PANTR	4.423e-65	30.30	General transcription factor IIH subunit 4 OS=Pan ...	[more]
sp\|Q92759\|TF2H4_HUMAN	4.423e-65	30.30	General transcription factor IIH subunit 4 OS=Homo...	[more]
sp\|Q6CLR2\|TFB2_KLULA	5.408e-53	26.06	General transcription and DNA repair factor IIH su...	[more]
sp\|Q75B51\|TFB2_ASHGO	1.421e-50	27.90	General transcription and DNA repair factor IIH su...	[more]

BLAST of General transcription factor IIH subunit 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A4Y7NK39	5.095e-77	33.26	General transcription factor IIH subunit 4 OS=Moin...	[more]
A0A4Y7M1Y0	9.014e-77	33.85	General transcription factor IIH subunit 4 OS=Daph...	[more]
A0A4Y7M1C8	6.736e-76	33.63	General transcription factor IIH subunit 4 OS=Daph...	[more]
A0A0R3UG68	1.247e-75	31.19	General transcription factor IIH subunit 4 OS=Meso...	[more]
A0A164RYY7	3.287e-75	32.81	General transcription factor IIH subunit 4 OS=Daph...	[more]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
gtf2h4	8.884e-63	28.79	general transcription factor IIH subunit 4 [Source...	[more]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
gtf2h4	1.402e-31	31.96	general transcription factor IIH, polypeptide 4 [S...	[more]
gtf2h4	8.739e-21	29.74	general transcription factor IIH, polypeptide 4 [S...	[more]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
TFB2	4.898e-44	26.00	Subunit of TFIIH and nucleotide excision repair fa...	[more]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO38911	2.601e-67	30.54	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of General transcription factor IIH subunit 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
gtf2h4	3.264e-61	28.32	general transcription factor IIH subunit 4 [Source...	[more]

BLAST of General transcription factor IIH subunit 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000022992.1	0.000e+0	100.00	SMESG000022992.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30033635 ID=SMED30033635|Name=General transcription factor IIH subunit 4|organism=Schmidtea mediterranea sexual|type=transcript|length=1982bp
ATTTATTAAAATAGTTTGATGAATATAGTGCCATTGAATCTCAAGGCGAA
ACGCAAAGACATGATATGAGATGTTTGAGATAATTTATTCACTAGATATC
TATGTGATAAATCAAGCCGTTACCTTTACGTGCTCTTCTATATAATTATT
TTGATGTTACATGTGATAAGTGTAATTTTTTTATTTATTGAATATAAAAT
TTGGTAATGATTTTAAATGTTCGGAGTTGTTTAGTAAATGTGAACGAATA
ATTGTATACTGGATAATTGTATCAAAAAACAATGATGTTTGATTATCATC
CAATGAAAACCTCTAATGAATTTCACAATAAAATTTTTTTTCCAACAAAT
AATTGATATTAATTGAGATTTCTTTGTCAACAAATGTTTATTATTTTTTA
GTAAATATTTTGAGATTTGAAAATGACATCTTGAATAAATTGTTTTAATA
TTTAAATATCGATGGAAAATTTTGAAAAGAGTTTATTACTTAATTTTATT
AAGACCTTAGATTCTCAAGATATTGAGAAAATTTTCCTTTCTCCATATAC
TTGCGCAGTTATTTTTCGTGAATTGTCAAATCTTAGTAAACACATCGTTT
GTCGTGTGTTATTTATTGACGAAACTGTGCGTAAGAGCCTTATAGCTTCA
TGGGTAAATAAAGATTATTCCTACATGTTAACAAAATCTCTTGAAGAACT
AAATGATTTGATTATATTTACAAGAGTCAGTAATGGACCTCAAGGTGAAT
GTATGCGCCTTAACAAAAAATTTCAAGAAAACCTCAAATATGCTCTTTTT
GGAGGAGAAACATGTACATTAGTCAAAGAATATAATGAATCGAATTTTAA
ATCAATTGCTTCGGATGTTTTAACCAAAAAAGCAATTGACAAATGGGAAT
CGGTTTTATCTTTAGTTGCTGGTATTATTCATAAAACATCGAACAATAAT
ATTGTTCGTAGTGTTATTGTGAATGCTGGTTACATCAAAAACGATGAAAT
TACAAATGAAGGATTTCAGTTTCTTTTATTACAAAAAAAGAAACAAGTTT
GGCAGTTTCTTATTTCTTATTTGGAATATATTAAGGAACCTTCAAGTATT
ATCGATATTCTACCGTTGCTATTCCAAATCGGTTGCGGATCAATTGATCA
AGTATATACTTGCACAACACCGACAAAGGCTCAACGCGAAATGTTGCAGC
ATTTTTTGGAATTCGGTTTCATCGTTTCTGAAACGGAACCGCATCTGGCA
TTCCATGTGACGCCGTTATTGGTCGATTTGCTGTCTGGCAATGATCGAAT
GTCACTAAAGGAAGGCCGTGACCAGTCTGCATCATCGATCATTAGCAGTT
TGGGATATATCATTATGGAATCAAGTGATCTCATATATGCTCACACAGAT
TCAAAATTACAAATTGCAGTCTTAGAAATGTTTTGCAAACTGCATAAACG
TTTCCCTTTCGCCGCTGCCTATCATATAACAGATCAATCGATTTTCAGGG
CGCTTGATAAAGGAATCACTTCAGATCAGATAATATCCTTTCTCATGAAC
AACGGCCATCCGGCAATGAAAGAAAAATCCGTTGTTCCGATCACGTTGGT
GGATAAAATCAAATTAGTGGAATTCAACCGCAACCGCACAAGGCCAGTTA
GTGGTTACATGTATCAAAATTTTACTAATCAATCTGAATTCGACACGCTC
GTCAATTTTGCTGAGCCTAAAAATGCGATTGCCTGGAAGAATCGCGAGAA
GCTCATGGTGATGGTATACGAAGACAGCCATATGATGATTCGTAAATTCT
GGAAAAATAGGAAGAATTCAGATTATCTTTGATGAAGAAATCTCTATGAT
CATTATAAATATAGCTCACCATTTAATCTTCAGATATTGTATTTTTATTT
GTTAATACACTTTCTATCGTGTTTTGTAAATTGTTGAATAATGATTCAAA
TTCCTCGAGTTTTCATATATTTATTAGCATGT

protein sequence of SMED30033635-orf-1

>SMED30033635-orf-1 ID=SMED30033635-orf-1|Name=SMED30033635-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=457bp
MENFEKSLLLNFIKTLDSQDIEKIFLSPYTCAVIFRELSNLSKHIVCRVL
FIDETVRKSLIASWVNKDYSYMLTKSLEELNDLIIFTRVSNGPQGECMRL
NKKFQENLKYALFGGETCTLVKEYNESNFKSIASDVLTKKAIDKWESVLS
LVAGIIHKTSNNNIVRSVIVNAGYIKNDEITNEGFQFLLLQKKKQVWQFL
ISYLEYIKEPSSIIDILPLLFQIGCGSIDQVYTCTTPTKAQREMLQHFLE
FGFIVSETEPHLAFHVTPLLVDLLSGNDRMSLKEGRDQSASSIISSLGYI
IMESSDLIYAHTDSKLQIAVLEMFCKLHKRFPFAAAYHITDQSIFRALDK
GITSDQIISFLMNNGHPAMKEKSVVPITLVDKIKLVEFNRNRTRPVSGYM
YQNFTNQSEFDTLVNFAEPKNAIAWKNREKLMVMVYEDSHMMIRKFWKNR
KNSDYL*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0001671	ATPase activator activity
GO:0004003	ATP-dependent DNA helicase activity

Vocabulary: **biological process**
Term	Definition
GO:0006281	DNA repair
GO:0006974	cellular response to DNA damage stimulus
GO:0032781	positive regulation of ATPase activity
GO:0006289	nucleotide-excision repair
GO:0032508	DNA duplex unwinding

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002109	X1 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR040662	Tfb2_C
IPR004598	TFIIH_p52/Tfb2

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus
GO:0000439	core TFIIH complex

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.30.70.2610		coord: 377..454 e-value: 2.4E-16 score: 61.6
IPR004598	Transcription factor TFIIH subunit p52/Tfb2	PFAM	PF03849	Tfb2	coord: 11..367 e-value: 2.7E-82 score: 276.6
IPR004598	Transcription factor TFIIH subunit p52/Tfb2	PANTHER	PTHR13152	TFIIH, POLYPEPTIDE 4	coord: 3..450
IPR040662	Transcription factor Tfb2, C-terminal domain	PFAM	PF18307	Tfb2_C	coord: 381..448 e-value: 2.3E-14 score: 53.5