SMED30033630

Overview


Name	SMED30033630
Smed ID	SMED30033630
Length (bp)	1743

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30033630 (SMED30033630) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30033630 (SMED30033630) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30033630 (SMED30033630) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30033630 (SMED30033630) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30033630

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 2

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
intestinal phagocyte	SMED30033630		PL030014B20F06	ncbi_smed_ests	PMID:23079596 Forsthoefel et al., 2012
prepharyngeal region	SMED30033630		SmedASXL_076758	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016

Note: Hover over icons to view figure legend

Alignments

SMED30033630 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.004539:17518..20574 +	8151
v31.009923:9867..12935 +	7809

Homology

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BLAST of SMED30033630 vs. Ensembl Zebrafish
Match: CR354395.2 (pep chromosome:GRCz11:12:13553730:13556536:-1 gene:ENSDARG00000101547.2 transcript:ENSDART00000166396.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR354395.2)

HSP 1 Score: 54.6842 bits (130), Expect = 1.713e-8
Identity = 33/117 (28.21%), Postives = 62/117 (52.99%), Query Frame = -3

Query: 1010 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEK-VIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYG--KIFTLRTEYKAITFMLSTKK 1351
            +    +AF ++K  FT  P+  +P+    FI++ DAS   +GAVL QR     +    A+    ++S E+   +  +ELLA+    + +  +  G  + FT+ T++K + ++LS K+
Sbjct:  442 SSEARQAFSDLKTQFTTAPILRHPNPDLPFIVEVDASNTGVGAVLSQRQGQPSKMYPCAFFSRKLTSAERNYDVGNRELLAMKLALEEWRHWLEGASQQFTILTDHKNLEYLLSAKR 558

HSP 2 Score: 24.2534 bits (51), Expect = 1.713e-8
Identity = 16/58 (27.59%), Postives = 24/58 (41.38%), Query Frame = -2

Query:  792 FDIKIKYRKTVNHANVDALSRSTFGTCTQCLLSQENTKTEKRKIWRLNML*KILSCKW 965
            FD  + YR    ++  DALSR          LS++ T      I   N+L  +   +W
Sbjct:  573 FDFIVTYRPGSKNSKADALSR----------LSEDKTPISDETIIPTNLL--VAPVQW 618

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BLAST of SMED30033630 vs. Ensembl Zebrafish
Match: BX511224.1 (pep chromosome:GRCz11:2:18017000:18022765:1 gene:ENSDARG00000113243.1 transcript:ENSDART00000186877.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511224.1)

HSP 1 Score: 57.7658 bits (138), Expect = 3.221e-8
Identity = 38/122 (31.15%), Postives = 63/122 (51.64%), Query Frame = -3

Query: 1010 TLIRRTENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRD-NNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGK--IFTLRTEYKAITFMLSTKK 1366
            T  R +   E AF  +K  F   P+   PD +R F+++ DAS   +GA+L QR  ++G     AY  H +SS E+   I  +ELLA+    + +  +  G    F + T++K + ++ S K+
Sbjct:  786 TPFRWSSAAEAAFSKLKGCFVSAPILIAPDPSRQFVVEVDASEVGVGAILSQRSASDGKVHPCAYFSHRLSSAERNYDIGNRELLAVKLALEEWRHWLEGSGVPFIVWTDHKNLEYIKSAKR 907

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BLAST of SMED30033630 vs. Ensembl Zebrafish
Match: BX511082.1 (pep chromosome:GRCz11:9:14291932:14297132:1 gene:ENSDARG00000113678.1 transcript:ENSDART00000183119.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511082.1)

HSP 1 Score: 53.5286 bits (127), Expect = 8.023e-8
Identity = 35/122 (28.69%), Postives = 63/122 (51.64%), Query Frame = -3

Query: 1010 TLIRRTENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQR-DNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGK--IFTLRTEYKAITFMLSTKK 1366
            T  R +   + AF  +K  F   P+   PD +R F+++ DAS   +GA+L QR  ++G     AY  H +++ E+   I  +ELLA+    + +  +  G    F + T++K + ++ S K+
Sbjct:  786 TPFRWSNAAQVAFDRLKSCFVSAPILIAPDPSRQFVVEVDASEVGVGAILSQRSSSDGKMHPCAYFSHRLNNAEQNYDIGNRELLAVKLALEEWRHWLEGSGVPFIVWTDHKNLEYIQSAKR 907

HSP 2 Score: 23.0978 bits (48), Expect = 8.023e-8
Identity = 10/21 (47.62%), Postives = 11/21 (52.38%), Query Frame = -2

Query:  903 FDIKIKYRKTVNHANVDALSR 965
            FD  I YR    +   DALSR
Sbjct:  922 FDFSISYRPGSKNVKPDALSR 942

[Close]

BLAST of SMED30033630 vs. Ensembl Zebrafish
Match: CR855320.1 (pep chromosome:GRCz11:1:7956030:7961696:1 gene:ENSDARG00000099359.2 transcript:ENSDART00000159655.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR855320.1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.256e-7
Identity = 37/122 (30.33%), Postives = 62/122 (50.82%), Query Frame = -3

Query: 1010 TLIRRTENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQR-DNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGK--IFTLRTEYKAITFMLSTKK 1366
            T  R +   E AF  +K  F   P+   PD +R F+++ DAS   +GA+L QR  ++G     AY  H +S+ E    I  +ELLA+    + +  +  G    F + T++K + ++ S K+
Sbjct:  788 TPFRWSSAAEAAFSKLKGCFVSAPILITPDPSRQFVVEVDASEVGVGAILSQRSSSDGKIHPCAYYSHRLSAAESNYDIGNRELLAVKLALEEWRHWLEGSGVPFIVWTDHKNLEYIKSAKR 909

[Close]

BLAST of SMED30033630 vs. Ensembl Zebrafish
Match: BX546500.1 (pep chromosome:GRCz11:23:12926092:12931693:-1 gene:ENSDARG00000086495.3 transcript:ENSDART00000122176.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX546500.1)

HSP 1 Score: 55.0694 bits (131), Expect = 2.360e-7
Identity = 36/119 (30.25%), Postives = 61/119 (51.26%), Query Frame = -3

Query: 1010 RRTENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQR-DNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGK--IFTLRTEYKAITFMLSTKK 1357
            R +   E AF  +K  F   P+   PD +R F+++ DAS   +GA+L QR  ++G     AY  H +S  E+   I  +ELLA+    + +  +  G    F + T++K + ++ S K+
Sbjct:  763 RWSSAAEAAFSKLKGCFVSAPILIAPDPSRQFVVEVDASEVGVGAILSQRSSSDGKIHPCAYFSHRLSPAERNYDIGNRELLAVKLALEEWRHWLEGSGVPFIVWTDHKNLEYIRSAKR 881

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BLAST of SMED30033630 vs. Ensembl Xenopus
Match: anxa6 (annexin A6 [Source:Xenbase;Acc:XB-GENE-989741])

HSP 1 Score: 78.1814 bits (191), Expect = 2.983e-16
Identity = 41/109 (37.61%), Postives = 57/109 (52.29%), Query Frame = -3

Query: 1025 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFM 1351
            T  CE A   +K+A    PV   PDF+R FIL TDAS   +GAVL Q +  G E  +AY    +   E       KE LAI +  Q    Y YG+ FT+ T++  ++++
Sbjct:  605 TPECESAMNALKQALASSPVLAAPDFSRRFILQTDASNFGLGAVLSQVNTYGEEHPVAYLSRKLLPREAAYATIEKECLAIVWALQKLQPYLYGREFTVVTDHNPLSWL 713

HSP 2 Score: 27.7202 bits (60), Expect = 2.983e-16
Identity = 12/35 (34.29%), Postives = 19/35 (54.29%), Query Frame = -2

Query:  903 NNCAISKVDQLSQYFDIKIKYRKTVNHANVDALSR 1007
            +N  + +   L Q ++  I++RK   H N D LSR
Sbjct:  719 DNGKLLRWSLLLQQYNFTIQHRKGKEHHNADGLSR 753

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BLAST of SMED30033630 vs. Ensembl Xenopus
Match: ENSXETT00000024810.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV464121.1:1437:3201:1 gene:ENSXETG00000014958.1 transcript:ENSXETT00000024810.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 70.8626 bits (172), Expect = 4.249e-15
Identity = 39/113 (34.51%), Postives = 55/113 (48.67%), Query Frame = -3

Query: 1025 LIRRTENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFM 1363
            ++  T  C  A   +K A    PV   PDF+R FI+ TDAS   IGAVL Q D  G E  I Y    +   E       KE LAI +  +    Y +G  FT+ T++  ++++
Sbjct:  151 VVTWTPECATAMSALKSALVNAPVLYAPDFSRGFIVHTDASTYGIGAVLSQVDEKGGEHPIIYLSRKLLPREVAYATIEKECLAIVWALKKLQPYLFGSAFTVVTDHNPLSWL 263

HSP 2 Score: 30.8018 bits (68), Expect = 4.249e-15
Identity = 13/30 (43.33%), Postives = 17/30 (56.67%), Query Frame = -2

Query:  882 QYFDIKIKYRKTVNHANVDALSRSTFGTCT 971
            Q F+  I++RK  +H N D LSR     CT
Sbjct:  281 QQFNFTIQHRKGSHHGNADGLSRRDGEDCT 310

[Close]

BLAST of SMED30033630 vs. Ensembl Xenopus
Match: ENSXETT00000031144.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:8:63282574:63284044:1 gene:ENSXETG00000009810.1 transcript:ENSXETT00000031144.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 69.3218 bits (168), Expect = 6.596e-12
Identity = 40/115 (34.78%), Postives = 62/115 (53.91%), Query Frame = -3

Query: 1010 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAI-YYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKK 1351
            T  CE AF  +KK  T+ PV  Y D  R ++L  DAS++ +G VL QR   G   V AY   +++  EK   + + E LA+ +      + + YG  F +RT+   +T++L+T K
Sbjct:   97 TSACEGAFVQLKKRLTEAPVLAYADAHRPYVLHVDASYEGLGGVLHQRYPEGLRPV-AYLSRSLAPSEKNYPVHKLEFLALKWAIVDKLHDFLYGVEFEVRTDNNPLTYILTTAK 210

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BLAST of SMED30033630 vs. Ensembl Xenopus
Match: ENSXETT00000011934.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:51966053:51967075:1 gene:ENSXETG00000005538.1 transcript:ENSXETT00000011934.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 62.3882 bits (150), Expect = 3.603e-10
Identity = 37/114 (32.46%), Postives = 59/114 (51.75%), Query Frame = -3

Query: 1010 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKK 1351
            T   E AF N+K +  + PV   P+F ++F++ TDAS   +GAVL Q   NG E  +AY    ++  E    I  +E LAI +  +    Y  G+ F L T++  + +M   ++
Sbjct:   79 TPEAEEAFLNLKASLCRYPVLIAPNFKKEFLVQTDASEVGLGAVLSQ-VVNGEEHPVAYLSRKLTPAECRYAIVERECLAIKWALESLRYYLLGRQFKLITDHAPLKWMAQNRE 191

[Close]

BLAST of SMED30033630 vs. Ensembl Xenopus
Match: ENSXETT00000006041.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:6:37317867:37322724:1 gene:ENSXETG00000003070.1 transcript:ENSXETT00000006041.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.8474 bits (146), Expect = 5.633e-9
Identity = 35/115 (30.43%), Postives = 61/115 (53.04%), Query Frame = -3

Query: 1010 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAI-YYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKK 1351
            TE C++AF  +K   T  PV  Y D T+ + L  DAS + +G +L Q  N    + +A+   ++S  E+     + E LA+ +      ++Y YG  F ++T+   +T++L+T K
Sbjct:  617 TEECDKAFDQLKYCLTHAPVLAYADATKPYTLHIDASREGLGGILYQEYNKEL-RPVAFISRSLSPSERNYPAHKLEFLALKWAVVDKLHEYLYGAEFEVQTDNNPLTYILTTAK 730

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BLAST of SMED30033630 vs. UniProt/SwissProt
Match: sp|P10394|POL4_DROME (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 8.110e-15
Identity = 41/111 (36.94%), Postives = 68/111 (61.26%), Query Frame = -3

Query: 1019 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLS 1351
            T+ C++AF ++K       +  YPDF+++F + TDAS    GAVL Q ++NG++  +AY   A +  E     T +EL AI++   HF  Y YGK FT++T+++ +T++ S
Sbjct:  587 TDECQKAFIHLKSQLINPTLLQYPDFSKEFCITTDASKQACGAVLTQ-NHNGHQLPVAYASRAFTKGESNKSTTEQELAAIHWAIIHFRPYIYGKHFTVKTDHRPLTYLFS 696

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BLAST of SMED30033630 vs. UniProt/SwissProt
Match: sp|P04323|POL3_DROME (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.812e-8
Identity = 34/105 (32.38%), Postives = 59/105 (56.19%), Query Frame = -3

Query: 1025 ERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFM 1339
            + AFK +K   ++ P+   PDFT+ F L TDAS   +GAVL Q   +G+   ++Y    ++ HE       KELLAI +  + F  Y  G+ F + ++++ ++++
Sbjct:  484 DSAFKKLKYLISEDPILKVPDFTKKFTLTTDASDVALGAVLSQ---DGHP--LSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYLLGRHFEISSDHQPLSWL 583

HSP 2 Score: 21.1718 bits (43), Expect = 2.812e-8
Identity = 11/21 (52.38%), Postives = 12/21 (57.14%), Query Frame = -2

Query:  903 FDIKIKYRKTVNHANVDALSR 965
            FD  IKY K   +   DALSR
Sbjct:  603 FDFDIKYIKGKENCVADALSR 623

[Close]

BLAST of SMED30033630 vs. UniProt/SwissProt
Match: sp|P20825|POL2_DROME (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.384e-7
Identity = 34/108 (31.48%), Postives = 59/108 (54.63%), Query Frame = -3

Query: 1010 AFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKK 1333
            AF+ +K    + P+   PDF + F+L TDAS   +GAVL Q   NG+   I++    ++ HE       KELLAI +  + F  Y  G+ F + ++++ + ++ + K+
Sbjct:  485 AFEKLKALIIRDPILQLPDFEKKFVLTTDASNLALGAVLSQ---NGHP--ISFISRTLNDHELNYSAIEKELLAIVWATKTFRHYLLGRQFLIASDHQPLRWLHNLKE 587

[Close]

BLAST of SMED30033630 vs. UniProt/SwissProt
Match: sp|Q8I7P9|POL5_DROME (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 1.718e-7
Identity = 36/113 (31.86%), Postives = 59/113 (52.21%), Query Frame = -3

Query: 1013 ENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGK-IFTLRTEYKAITFMLSTK 1348
            E   ++F ++K       +  +P FT+ F L TDAS   IGAVL Q D+ G ++ IAY   +++  E+      KE+LAI +   +   Y YG     + T+++ +TF L  +
Sbjct:  407 ETALQSFNDLKSILCSSEILAFPCFTKPFHLTTDASNWAIGAVLSQ-DDQGRDRPIAYISRSLNKTEENYATIEKEMLAIIWSLDNLRAYLYGAGTIKVYTDHQPLTFALGNR 518

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BLAST of SMED30033630 vs. UniProt/SwissProt
Match: sp|Q7LHG5|YI31B_YEAST (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 55.4546 bits (132), Expect = 1.357e-6
Identity = 43/129 (33.33%), Postives = 64/129 (49.61%), Query Frame = -3

Query:  968 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDN-NGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKKSITVQFQR*INYLS 1351
            TE  ++A + +K A    PV    +   ++ L TDAS D IGAVL + DN N    V+ Y   ++ S +K       ELL I     HF    +GK FTLRT++ ++   L  K     + QR ++ L+
Sbjct:  885 TEKQDKAIEKLKAALCNSPVLVPFNNKANYRLTTDASKDGIGAVLEEVDNKNKLVGVVGYFSKSLESAQKNYPAGELELLGIIKALHHFRYMLHGKHFTLRTDHISL-LSLQNKNEPARRVQRWLDDLA 1012

[Close]

BLAST of SMED30033630 vs. TrEMBL
Match: A0A087T3I4 (Retrovirus-related Pol polyprotein from transposon 412 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_13332 PE=4 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 1.172e-24
Identity = 54/113 (47.79%), Postives = 77/113 (68.14%), Query Frame = -3

Query: 1013 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTK 1351
            T+ CE AFKN+K+A T  P+  YP   R FILDTDAS +++GAVL Q +  G E+V+AY    +S  E+  C+TRKELLAI    +HF+ Y YG+ F LRT++ ++ ++L+ K
Sbjct:  951 TKECENAFKNLKEALTSAPILTYPKLDRPFILDTDASNESVGAVLSQ-EIEGQERVVAYWSKCLSKPERNYCVTRKELLAIVKAVEHFHHYLYGRKFLLRTDHASLAWLLNFK 1062

HSP 2 Score: 32.3426 bits (72), Expect = 1.172e-24
Identity = 15/31 (48.39%), Postives = 21/31 (67.74%), Query Frame = -2

Query:  903 ISKVDQLSQYFDIKIKYRKTVNHANVDALSR 995
            I++  Q  Q +DI I++RK  +H N DALSR
Sbjct: 1068 IARWIQRLQEYDIIIRHRKGQSHGNADALSR 1098

[Close]

BLAST of SMED30033630 vs. TrEMBL
Match: A0A087SUZ9 (Retrovirus-related Pol polyprotein from transposon 412 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_16630 PE=4 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 1.516e-24
Identity = 54/113 (47.79%), Postives = 77/113 (68.14%), Query Frame = -3

Query: 1013 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTK 1351
            T+ CE AFKN+K+A T  P+  YP   R FILDTDAS +++GAVL Q +  G E+V+AY    +S  E+  C+TRKELLAI    +HF+ Y YG+ F LRT++ ++ ++L+ K
Sbjct:  937 TKECENAFKNLKEALTSAPILTYPQLDRPFILDTDASNESVGAVLSQ-EIEGQERVVAYWSKCLSKPERNYCVTRKELLAIVKAVEHFHHYLYGRKFLLRTDHASLAWLLNFK 1048

HSP 2 Score: 32.3426 bits (72), Expect = 1.516e-24
Identity = 15/31 (48.39%), Postives = 21/31 (67.74%), Query Frame = -2

Query:  903 ISKVDQLSQYFDIKIKYRKTVNHANVDALSR 995
            I++  Q  Q +DI I++RK  +H N DALSR
Sbjct: 1054 IARWIQRLQEYDITIRHRKGQSHGNADALSR 1084

[Close]

BLAST of SMED30033630 vs. TrEMBL
Match: A0A4Y2FYJ1 (Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ventricosus OX=182803 GN=TY3B-I_151 PE=4 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.881e-24
Identity = 53/114 (46.49%), Postives = 77/114 (67.54%), Query Frame = -3

Query: 1010 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKK 1351
            TE CE++F ++K+A T  P+  YP   +DFILDTDAS + IGAVL Q   N  E+VIAY   ++   E+  C+TRKELLAI    +HF+ Y YG+ F LRT++ ++ ++L+ K+
Sbjct:  899 TEECEKSFNSLKQALTSSPILTYPRTDKDFILDTDASNEGIGAVLSQNIGN-EERVIAYFSKSLGKPERNYCVTRKELLAIVKSIEHFHHYLYGQKFLLRTDHASLRWLLNFKE 1011

HSP 2 Score: 37.7354 bits (86), Expect = 1.881e-24
Identity = 17/41 (41.46%), Postives = 26/41 (63.41%), Query Frame = -2

Query:  876 ISKVDQLSQYFDIKIKYRKTVNHANVDALSRSTFG-TCTQC 995
            I++  Q  Q +D +I++RK  +H N DALSR  +G +C  C
Sbjct: 1016 IARWIQRLQEYDFEIQHRKGTSHGNADALSRRPWGESCKHC 1056

[Close]

BLAST of SMED30033630 vs. TrEMBL
Match: A0A087T293 (Retrovirus-related Pol polyprotein from transposon 412 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_23274 PE=4 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 2.126e-24
Identity = 54/113 (47.79%), Postives = 76/113 (67.26%), Query Frame = -3

Query: 1013 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTK 1351
            TE CE++F  +K A T  PV  YP   + FILDTDAS +++GAVL Q  + G E+VIAY    +S  E+  C+TRKELLAI    +HF+ Y YG+ F LRT++ ++T++L+ K
Sbjct:  967 TEECEKSFNELKTALTSTPVLAYPQIDKQFILDTDASNESVGAVLSQMID-GEERVIAYWSKCLSKPERNYCVTRKELLAIVKAIEHFHHYLYGRKFLLRTDHASLTWLLNFK 1078

HSP 2 Score: 33.113 bits (74), Expect = 2.126e-24
Identity = 15/31 (48.39%), Postives = 21/31 (67.74%), Query Frame = -2

Query:  903 ISKVDQLSQYFDIKIKYRKTVNHANVDALSR 995
            I++  Q  Q +D +IK+RK  +H N DALSR
Sbjct: 1084 IARWIQRLQEYDFEIKHRKGSSHGNADALSR 1114

[Close]

BLAST of SMED30033630 vs. TrEMBL
Match: A0A4Y2X454 (Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ventricosus OX=182803 GN=TY3B-I_1466 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 3.964e-24
Identity = 53/115 (46.09%), Postives = 78/115 (67.83%), Query Frame = -3

Query: 1007 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKKS 1351
            T+ CE++F ++K+A T  P+  YP   +DFILDTDAS + IGAVL Q   NG E+VIAY   ++   E+  C+TRKELLAI    +HF+ Y YG+ F LRT++ ++ ++L+ K+ 
Sbjct:  532 TDECEKSFNSLKQALTSSPILTYPRTDKDFILDTDASNEGIGAVLSQNTGNG-ERVIAYFSKSLGKPERNYCVTRKELLAIVKSIEHFHHYLYGRKFLLRTDHASLRWLLNFKEP 645

HSP 2 Score: 34.6538 bits (78), Expect = 3.964e-24
Identity = 17/41 (41.46%), Postives = 25/41 (60.98%), Query Frame = -2

Query:  876 ISKVDQLSQYFDIKIKYRKTVNHANVDALSRST-FGTCTQC 995
            I++  Q  Q +D +I++RK  +H N DALSR     +C QC
Sbjct:  649 IARWIQRLQEYDFEIQHRKGTSHGNADALSRRPCKESCKQC 689

[Close]

BLAST of SMED30033630 vs. Ensembl Cavefish
Match: ENSAMXT00000038919.1 (pep primary_assembly:Astyanax_mexicanus-2.0:25:7611858:7613942:-1 gene:ENSAMXG00000031762.1 transcript:ENSAMXT00000038919.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 98.9821 bits (245), Expect = 8.911e-24
Identity = 50/115 (43.48%), Postives = 72/115 (62.61%), Query Frame = -3

Query: 1007 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKKS 1351
            T  C+ AF+ +K+  T  PV GYP  T + ILDTDAS   IGAVL Q  N+  E+V+AY    +S  E+  C TR+ELLA+  F  HF QY  G+ FT+RT++ ++ +++  K+ 
Sbjct:  106 TAECQEAFEELKRRLTSAPVLGYPLDTGNIILDTDASNFGIGAVLSQVQND-EERVLAYGSRRLSQTEQNYCTTRRELLAVVEFTSHFRQYLLGRTFTVRTDHSSLRWLMRMKEP 219

HSP 2 Score: 31.187 bits (69), Expect = 8.911e-24
Identity = 13/48 (27.08%), Postives = 26/48 (54.17%), Query Frame = -2

Query:  843 VDQLSQYFDIKIKYRKTVNHANVDALSRSTFGTCTQCLLSQENTKTEK 986
            +++L +Y D K+ +R    H N DA+SR        C++   ++ +E+
Sbjct:  227 LEKLGEY-DFKVIHRPGAQHCNADAMSRRPCRKSCPCMVPDPSSSSER 273

[Close]

BLAST of SMED30033630 vs. Ensembl Cavefish
Match: ENSAMXT00000039384.1 (pep primary_assembly:Astyanax_mexicanus-2.0:25:21146036:21149703:-1 gene:ENSAMXG00000035834.1 transcript:ENSAMXT00000039384.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 93.9745 bits (232), Expect = 5.754e-22
Identity = 45/109 (41.28%), Postives = 69/109 (63.30%), Query Frame = -3

Query: 1025 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFM 1351
            T+ C+ AF+ +K      P+  +PD  + FILDTDAS   IGA+L Q+  +G+EKV+AY   A+S  EK    T+KELLA+ +F  +F  Y  G+ F LRT++ ++ ++
Sbjct:  119 TQECQTAFQTLKDKLVSAPILTFPDPGQTFILDTDASDVAIGAILSQK-IDGFEKVVAYASRALSRQEKNYATTKKELLAVVHFTSYFRHYLLGRRFLLRTDHSSLRWL 226

HSP 2 Score: 30.4166 bits (67), Expect = 5.754e-22
Identity = 13/28 (46.43%), Postives = 20/28 (71.43%), Query Frame = -2

Query:  903 VDQLSQYFDIKIKYRKTVNHANVDALSR 986
            ++QL+Q FD +I++R    H N D+LSR
Sbjct:  240 LEQLAQ-FDYEIEHRPGKKHVNADSLSR 266

[Close]

BLAST of SMED30033630 vs. Ensembl Cavefish
Match: ENSAMXT00000039316.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:30933509:30934681:1 gene:ENSAMXG00000029182.1 transcript:ENSAMXT00000039316.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 93.2041 bits (230), Expect = 2.078e-21
Identity = 45/111 (40.54%), Postives = 70/111 (63.06%), Query Frame = -3

Query: 1025 RRTENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFM 1357
            + T+ C+ AF+ +K      P+  +PD  + FILDTDAS   IGA+L Q+  +G+EKV+AY   A+S  EK    T+KELLA+ +F  +F  Y  G+ F LRT++ ++ ++
Sbjct:  129 KWTQECQTAFQTLKDKLVSAPILTFPDPGQTFILDTDASDVAIGAILSQK-IDGFEKVVAYASRALSRQEKNYATTKKELLAVVHFTSYFRHYLLGRRFLLRTDHSSLRWL 238

HSP 2 Score: 29.261 bits (64), Expect = 2.078e-21
Identity = 13/28 (46.43%), Postives = 20/28 (71.43%), Query Frame = -2

Query:  903 VDQLSQYFDIKIKYRKTVNHANVDALSR 986
            ++QL+Q FD +I++R    H N D+LSR
Sbjct:  252 LEQLAQ-FDYEIEHRPGKKHVNADSLSR 278

[Close]

BLAST of SMED30033630 vs. Ensembl Cavefish
Match: ENSAMXT00000048272.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000106.1:219951:223307:1 gene:ENSAMXG00000032559.1 transcript:ENSAMXT00000048272.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 97.4413 bits (241), Expect = 1.282e-20
Identity = 48/115 (41.74%), Postives = 73/115 (63.48%), Query Frame = -3

Query: 1007 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKKS 1351
            T  C+ AF+ +K + T  PV GYP    + ILDTDAS   IGAVL Q   +G E+V+AY    +SS E+  C TR+ELLA+  F +HF QY  G+ F +R+++ ++ ++++ K+ 
Sbjct:  345 TPECQTAFEELKSSLTSTPVLGYPRDHGNLILDTDASNFGIGAVLSQV-QDGAERVLAYGSRRLSSTEQNYCTTRRELLAVVEFTRHFRQYLLGRPFIVRSDHSSLRWLVNMKEP 458

[Close]

BLAST of SMED30033630 vs. Ensembl Cavefish
Match: ENSAMXT00000052614.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001686.1:18497:23810:-1 gene:ENSAMXG00000038033.1 transcript:ENSAMXT00000052614.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 97.0561 bits (240), Expect = 1.350e-20
Identity = 48/115 (41.74%), Postives = 73/115 (63.48%), Query Frame = -3

Query: 1007 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKKS 1351
            T  C+ AF+ +K + T  PV GYP    + ILDTDAS   IGAVL Q   +G E+V+AY    +SS E+  C TR+ELLA+  F +HF QY  G+ F +R+++ ++ ++++ K+ 
Sbjct:  170 TPECQAAFEELKSSLTSTPVLGYPRDHGNLILDTDASNFGIGAVLSQV-QDGAERVLAYGSRRLSSTEQNYCTTRRELLAVVEFTRHFRQYLLGRPFIVRSDHSSLRWLVNIKEP 283

[Close]

BLAST of SMED30033630 vs. Ensembl Medaka
Match: ENSORLT00000036827.1 (pep primary_assembly:ASM223467v1:16:28613823:28617539:1 gene:ENSORLG00000023550.1 transcript:ENSORLT00000036827.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 93.2041 bits (230), Expect = 1.382e-20
Identity = 49/115 (42.61%), Postives = 72/115 (62.61%), Query Frame = -3

Query: 1007 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKKS 1351
            T+ CE+AF ++KKA T  P+   PD    FILDTDAS   +GAVL Q  + G E+V+AY    +S  E+  C+TR+ELLA+     HF  Y  G  FT+RT++ A+ ++++ K+ 
Sbjct:  540 TQECEQAFSSLKKALTNSPILTPPDPKLPFILDTDASDVGMGAVLSQMGSAG-ERVVAYFSKTLSKAERRYCVTRRELLAVVKAIGHFRYYLCGLPFTVRTDHSALQWLMTFKEP 653

HSP 2 Score: 26.1794 bits (56), Expect = 1.382e-20
Identity = 9/21 (42.86%), Postives = 13/21 (61.90%), Query Frame = -2

Query:  903 FDIKIKYRKTVNHANVDALSR 965
            F   +++R    HAN DA+SR
Sbjct:  667 FSFTVEHRPGSRHANADAMSR 687

[Close]

BLAST of SMED30033630 vs. Ensembl Medaka
Match: ENSORLT00000040305.1 (pep primary_assembly:ASM223467v1:1:25459511:25466649:-1 gene:ENSORLG00000028409.1 transcript:ENSORLT00000040305.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 90.5077 bits (223), Expect = 1.342e-19
Identity = 45/114 (39.47%), Postives = 70/114 (61.40%), Query Frame = -3

Query: 1010 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKK 1351
            T  C+ AF+ +K+  T  P+ GYP  + + +LDTDAS   +GAVL Q    G E+V+AY    +++ E+  C TR+ELLA+  F  HF QY  G+ F LRT++ ++ ++   K+
Sbjct: 1099 TTECQEAFEELKERLTSAPILGYPLDSGELLLDTDASDWGVGAVLSQV-QGGEERVLAYGSRRLTTTEQNYCTTRRELLAVVEFTSHFRQYLLGRSFVLRTDHSSLRWLTRLKE 1211

HSP 2 Score: 25.7942 bits (55), Expect = 1.342e-19
Identity = 14/46 (30.43%), Postives = 24/46 (52.17%), Query Frame = -2

Query:  849 VDQLSQYFDIKIKYRKTVNHANVDALSRSTFGTCTQCLLSQENTKT 986
            +++L++Y D ++ +R    H N DALSR        C L   +T +
Sbjct: 1220 LEKLAEY-DFQVLHRPGKVHQNADALSRRPCRELCACKLPDPSTDS 1264

[Close]

BLAST of SMED30033630 vs. Ensembl Medaka
Match: ENSORLT00000040051.1 (pep primary_assembly:ASM223467v1:1:37354481:37359289:-1 gene:ENSORLG00000026212.1 transcript:ENSORLT00000040051.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 75.0998 bits (183), Expect = 2.332e-15
Identity = 41/117 (35.04%), Postives = 65/117 (55.56%), Query Frame = -3

Query: 1007 RRTENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKKS 1357
            R TE C+R+F  +K+A  K P+   PD +  F + TDAS   +GAVL Q  + G E+VIAY    +   E+    + KE LA+ +  + +  Y  G  FT+ T++ A+T+  +  K+
Sbjct:  997 RWTEECQRSFNQLKQALAKAPILVQPDLSLPFEVHTDASDLGLGAVLAQMTSEG-ERVIAYASRGLKGAERNYSTSEKECLAVVWAVEKWQHYLEGAEFTVFTDHAALTWAFNCPKT 1112

HSP 2 Score: 26.9498 bits (58), Expect = 2.332e-15
Identity = 14/34 (41.18%), Postives = 19/34 (55.88%), Query Frame = -2

Query:  885 QYFDIKIKYRKTVNHANVDALSR-----STFGTC 971
            Q F  K++YRK +++   DALSR     S   TC
Sbjct: 1124 QRFQFKVQYRKGLHNVVPDALSRAMVSPSDIATC 1157

[Close]

BLAST of SMED30033630 vs. Ensembl Medaka
Match: ENSORLT00000034492.1 (pep primary_assembly:ASM223467v1:15:13490768:13497120:-1 gene:ENSORLG00000024904.1 transcript:ENSORLT00000034492.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 70.8626 bits (172), Expect = 2.588e-12
Identity = 41/113 (36.28%), Postives = 59/113 (52.21%), Query Frame = -3

Query: 1013 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTK 1351
            TE CERAF  +K      PV   PDF + F++  DAS   +GAVL+Q D  G E  + Y    +   E    +  KE LAI +  +    Y  G+ F L T+++A+T++ S K
Sbjct: 1310 TEECERAFNALKACLCSTPVLRSPDFNKRFLVQVDASAVGLGAVLVQGD-PGQEHPVLYLSRKLLPRETRYSVVEKEALAIKWALERLRYYLVGREFDLETDHRALTWINSMK 1421

[Close]

BLAST of SMED30033630 vs. Ensembl Medaka
Match: ENSORLT00000029819.1 (pep primary_assembly:ASM223467v1:24:6358775:6362914:-1 gene:ENSORLG00000022361.1 transcript:ENSORLT00000029819.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.8474 bits (146), Expect = 3.985e-12
Identity = 38/117 (32.48%), Postives = 61/117 (52.14%), Query Frame = -3

Query: 1013 TENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*C---ITRKELLAI-YYFCQHFNQYFYGKIFTLRTEYKAITFMLSTK 1351
            TE C+R+F+ +K   T  PV  Y DF+R FIL+ DAS   +GAVL Q +  G  + IAY    +   E+        R E LA+ +   + F +Y  G+   + T+   ++++ + K
Sbjct:  626 TEECQRSFEALKAKLTTTPVLAYADFSRPFILEVDASNGGLGAVLSQ-EQEGKVRPIAYASRGLRPTERNPVNYSSMRLEFLALKWAVAEKFREYLLGQKCIVYTDNNPLSYLSTAK 741

HSP 2 Score: 30.0314 bits (66), Expect = 3.985e-12
Identity = 12/24 (50.00%), Postives = 17/24 (70.83%), Query Frame = -2

Query:  894 FDIKIKYRKTVNHANVDALSRSTF 965
            FD++I+YR   ++ N DALSR  F
Sbjct:  756 FDLEIRYRSGRSNRNADALSRQHF 779

[Close]

BLAST of SMED30033630 vs. Planmine SMEST
Match: SMESG000016546.1 (SMESG000016546.1)

HSP 1 Score: 203.756 bits (517), Expect = 4.987e-58
Identity = 97/132 (73.48%), Postives = 111/132 (84.09%), Query Frame = -3

Query:  953 ENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKKSITVQFQR*INYLSILTSK 1348
            ENCE+AF++MKKA T+ PV GYPDFTRDFI+DTD+SFDTIG VL Q+DNNGYEKVIAY  HA+S+HEKG CI RKELLAIYYFCQHFN + YGK FTLRT+ KAITFMLSTKK IT QF+  IN+L +   K
Sbjct:  383 ENCEKAFEDMKKALTESPVLGYPDFTRDFIIDTDSSFDTIGDVLSQKDNNGYEKVIAYGSHAMSTHEKGYCIKRKELLAIYYFCQHFNHHLYGKRFTLRTDLKAITFMLSTKKPITAQFKTWINHLIVEIDK 514

[Close]

BLAST of SMED30033630 vs. Planmine SMEST
Match: SMESG000029259.1 (SMESG000029259.1)

HSP 1 Score: 208.764 bits (530), Expect = 3.712e-57
Identity = 116/241 (48.13%), Postives = 150/241 (62.24%), Query Frame = -3

Query:  683 IRRTENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKKSITVQFQR*INYLSILTSKLSIEK---------------Q*IMQM*MHYREVLLGLVHNAY*VRRIQRQKKGKYGV*TCCRKY*VVNGNKEIRQIKNEVAQGSCWKYMLKEEMVMTIEGKLWVP 1360
            IR TE CE+AF  MK+A    PV  +PDF ++FILDTDASFDTIGAVL Q+D  G+E VIAY  HA+SSHEKG CITRKELLAIYYFC+HFN Y YGK F LRT++KAITFM++TKK IT QFQ  INYLS L  K+   K                  +Q  M + +   G +      R +    +G Y       K+   N N E+++IKN++    C K++++   V+T +GK+W+P
Sbjct: 2993 IRWTEMCEKAFGEMKEALITAPVLVFPDFRKEFILDTDASFDTIGAVLSQKDEKGHEHVIAYGSHAMSSHEKGYCITRKELLAIYYFCKHFNHYLYGKRFVLRTDHKAITFMVTTKKPITAQFQTWINYLSSLDIKMEYRKGTSHTNADMLSRKTCGTCVQCMMEHEDAKTGKIK----TRILTVTAEGGYN------KW--QNDNMEVQEIKNKLENKDC-KFIMENNTVLTKQGKIWIP 3220

HSP 2 Score: 55.0694 bits (131), Expect = 1.703e-7
Identity = 28/62 (45.16%), Postives = 36/62 (58.06%), Query Frame = -2

Query:  780 FDIKIKYRKTVNHANVDALSRSTFGTCTQCLLSQENTKTEKRKIWRLNML*KILSCKWQQGN 965
             DIK++YRK  +H N D LSR T GTC QC++  E+ KT K K   L +  +    KWQ  N
Sbjct: 3125 LDIKMEYRKGTSHTNADMLSRKTCGTCVQCMMEHEDAKTGKIKTRILTVTAEGGYNKWQNDN 3186

[Close]

BLAST of SMED30033630 vs. Planmine SMEST
Match: SMESG000038955.1 (SMESG000038955.1)

HSP 1 Score: 121.709 bits (304), Expect = 2.352e-31
Identity = 64/132 (48.48%), Postives = 84/132 (63.64%), Query Frame = -3

Query:  968 LIRRTENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKKSITVQFQR*INYLS 1363
            +I  TE C  +F N+KK   + P+  YP   R F++DTDASF  IGAVL Q   +  E VIAY    ++ HE G C+T KELLA++ F  HF QY YG+ F  RT++KA+TFM +TKK I+ QFQ  +  LS
Sbjct:  362 MIVWTEECRNSFINLKKLLCEAPILEYPRADRLFVIDTDASFGAIGAVLSQIKEDCTEVVIAYGSRHLTKHEMGHCVTIKELLALHEFIVHFRQYLYGRRFVARTDHKALTFMNTTKKPISPQFQTWMANLS 493

HSP 2 Score: 33.8834 bits (76), Expect = 2.352e-31
Identity = 18/54 (33.33%), Postives = 28/54 (51.85%), Query Frame = -2

Query:  816 LSQYFDIKIKYRKTVNHANVDALSRSTFGTCTQCLLSQENTKTEKRKIWRLNML 977
            LS++ D  ++YRK   H N D  SR     C+ CL+  ++ K  K +   +N L
Sbjct:  492 LSEH-DFALQYRKGEEHGNADGKSRLNNTKCSHCLMENKDAKEAKCRTRYINSL 544

[Close]

BLAST of SMED30033630 vs. Planmine SMEST
Match: SMESG000075438.1 (SMESG000075438.1)

HSP 1 Score: 116.701 bits (291), Expect = 6.277e-30
Identity = 56/121 (46.28%), Postives = 79/121 (65.29%), Query Frame = -3

Query: 1001 LIRRTENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKKSIT 1363
            +I  TE C ++F N+KK   + P+  YP   R FI+DT+A F  +GAVL Q   +G ++VI Y    ++ HE G C+TRKELLA++ F  HF QY YG+ F  RT++KA+TFM +TK+ I 
Sbjct:  410 MIVWTEECRKSFINLKKLLCEAPILEYPRVDRLFIIDTNAGFGALGAVLSQIKEDGTKRVITYGSRHLTKHEMGYCVTRKELLALHEFIVHFRQYLYGRRFVARTDHKALTFMNTTKQPIN 530

HSP 2 Score: 33.8834 bits (76), Expect = 6.277e-30
Identity = 16/45 (35.56%), Postives = 24/45 (53.33%), Query Frame = -2

Query:  843 LSQYFDIKIKYRKTVNHANVDALSRSTFGTCTQCLLSQENTKTEK 977
            LS+Y D  ++YRK   H N D +SR     C+ C +  ++ K  K
Sbjct:  540 LSEY-DFALQYRKGEEHGNADNISRLDNTICSHCQMEHKDAKKAK 583

[Close]

BLAST of SMED30033630 vs. Planmine SMEST
Match: SMESG000029294.1 (SMESG000029294.1)

HSP 1 Score: 119.013 bits (297), Expect = 1.136e-29
Identity = 60/125 (48.00%), Postives = 82/125 (65.60%), Query Frame = -3

Query:  989 LIRRTENCERAFKNMKKAFTKLPV*GYPDFTRDFILDTDASFDTIGAVLLQRDNNGYEKVIAYE*HAISSHEKG*CITRKELLAIYYFCQHFNQYFYGKIFTLRTEYKAITFMLSTKKSITVQFQ 1363
            +I  TE C + F ++KK   K P+  YP   R FI+DT+ASF  IG+VL Q   +G E VIAY    ++ +E G C+TRKELLA++ F  HF+QY YG+ F  RT +KA+TFM + KK ++ QFQ
Sbjct:  191 MIVWTEECRKGFISLKKLLCKAPMLEYPMADRIFIIDTEASFGAIGSVLSQIKEDGTEGVIAYGSRHLAKYEMGYCVTRKELLALHKFIVHFSQYLYGRRFVARTNHKALTFMNTAKKPLSPQFQ 315

HSP 2 Score: 30.8018 bits (68), Expect = 1.136e-29
Identity = 12/41 (29.27%), Postives = 21/41 (51.22%), Query Frame = -2

Query:  843 FDIKIKYRKTVNHANVDALSRSTFGTCTQCLLSQENTKTEK 965
            ++  ++YRK   H N D +SR     C+ C +  ++ K  K
Sbjct:  324 YNFALQYRKGEEHGNADGMSRLNNTKCSYCQMEHKDAKEAK 364

The following BLAST results are available for this feature:

BLAST of SMED30033630 vs. Ensembl Human
BLAST of SMED30033630 vs. Ensembl Celegans
BLAST of SMED30033630 vs. Ensembl Fly
BLAST of SMED30033630 vs. Ensembl Zebrafish
BLAST of SMED30033630 vs. Ensembl Xenopus
BLAST of SMED30033630 vs. Ensembl Mouse
BLAST of SMED30033630 vs. UniProt/SwissProt
BLAST of SMED30033630 vs. TrEMBL
BLAST of SMED30033630 vs. Ensembl Cavefish
BLAST of SMED30033630 vs. Ensembl Sea Lamprey
BLAST of SMED30033630 vs. Ensembl Yeast
BLAST of SMED30033630 vs. Ensembl Nematostella
BLAST of SMED30033630 vs. Ensembl Medaka
BLAST of SMED30033630 vs. Planmine SMEST

BLAST of SMED30033630 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30033630 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30033630 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30033630 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CR354395.2	1.713e-8	28.21	pep chromosome:GRCz11:12:13553730:13556536:-1 gene...	[more]
BX511224.1	3.221e-8	31.15	pep chromosome:GRCz11:2:18017000:18022765:1 gene:E...	[more]
BX511082.1	8.023e-8	28.69	pep chromosome:GRCz11:9:14291932:14297132:1 gene:E...	[more]
CR855320.1	1.256e-7	30.33	pep chromosome:GRCz11:1:7956030:7961696:1 gene:ENS...	[more]
BX546500.1	2.360e-7	30.25	pep chromosome:GRCz11:23:12926092:12931693:-1 gene...	[more]

BLAST of SMED30033630 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
anxa6	2.983e-16	37.61	annexin A6 [Source:Xenbase;Acc:XB-GENE-989741]	[more]
ENSXETT00000024810.1	4.249e-15	34.51	pep primary_assembly:Xenopus_tropicalis_v9.1:KV464...	[more]
ENSXETT00000031144.1	6.596e-12	34.78	pep primary_assembly:Xenopus_tropicalis_v9.1:8:632...	[more]
ENSXETT00000011934.1	3.603e-10	32.46	pep primary_assembly:Xenopus_tropicalis_v9.1:3:519...	[more]
ENSXETT00000006041.1	5.633e-9	30.43	pep primary_assembly:Xenopus_tropicalis_v9.1:6:373...	[more]

BLAST of SMED30033630 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30033630 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P10394\|POL4_DROME	8.110e-15	36.94	Retrovirus-related Pol polyprotein from transposon...	[more]
sp\|P04323\|POL3_DROME	2.812e-8	32.38	Retrovirus-related Pol polyprotein from transposon...	[more]
sp\|P20825\|POL2_DROME	1.384e-7	31.48	Retrovirus-related Pol polyprotein from transposon...	[more]
sp\|Q8I7P9\|POL5_DROME	1.718e-7	31.86	Retrovirus-related Pol polyprotein from transposon...	[more]
sp\|Q7LHG5\|YI31B_YEAST	1.357e-6	33.33	Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomy...	[more]

BLAST of SMED30033630 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A087T3I4	1.172e-24	47.79	Retrovirus-related Pol polyprotein from transposon...	[more]
A0A087SUZ9	1.516e-24	47.79	Retrovirus-related Pol polyprotein from transposon...	[more]
A0A4Y2FYJ1	1.881e-24	46.49	Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ve...	[more]
A0A087T293	2.126e-24	47.79	Retrovirus-related Pol polyprotein from transposon...	[more]
A0A4Y2X454	3.964e-24	46.09	Transposon Ty3-I Gag-Pol polyprotein OS=Araneus ve...	[more]

BLAST of SMED30033630 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000038919.1	8.911e-24	43.48	pep primary_assembly:Astyanax_mexicanus-2.0:25:761...	[more]
ENSAMXT00000039384.1	5.754e-22	41.28	pep primary_assembly:Astyanax_mexicanus-2.0:25:211...	[more]
ENSAMXT00000039316.1	2.078e-21	40.54	pep primary_assembly:Astyanax_mexicanus-2.0:15:309...	[more]
ENSAMXT00000048272.1	1.282e-20	41.74	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000052614.1	1.350e-20	41.74	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]

BLAST of SMED30033630 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30033630 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30033630 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30033630 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000036827.1	1.382e-20	42.61	pep primary_assembly:ASM223467v1:16:28613823:28617...	[more]
ENSORLT00000040305.1	1.342e-19	39.47	pep primary_assembly:ASM223467v1:1:25459511:254666...	[more]
ENSORLT00000040051.1	2.332e-15	35.04	pep primary_assembly:ASM223467v1:1:37354481:373592...	[more]
ENSORLT00000034492.1	2.588e-12	36.28	pep primary_assembly:ASM223467v1:15:13490768:13497...	[more]
ENSORLT00000029819.1	3.985e-12	32.48	pep primary_assembly:ASM223467v1:24:6358775:636291...	[more]

BLAST of SMED30033630 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000016546.1	4.987e-58	73.48	SMESG000016546.1	[more]
SMESG000029259.1	3.712e-57	48.13	SMESG000029259.1	[more]
SMESG000038955.1	2.352e-31	48.48	SMESG000038955.1	[more]
SMESG000075438.1	6.277e-30	46.28	SMESG000075438.1	[more]
SMESG000029294.1	1.136e-29	48.00	SMESG000029294.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30033630 ID=SMED30033630|Name=SMED30033630|organism=Schmidtea mediterranea sexual|type=transcript|length=1743bp
ATTTCATTGTCAAATTATGATACGAATTGCAATTGTGTTTATAATTGTTG
CAAGTTCTTTTAATCAAATTAACTGCGGAATATTAGGCATTCTCGGTTAC
GGAGCATGCCAATCTGTGTGTAATGCGGGATATGTCGCGTGTTTAGGGTC
CTTGGGACTAACAGCGGTTAAAACATTGAAAATTTTATGTAATGATTCTA
TTTTATATTTTACAGGGGCGCTTAACGCTGGGGCTGGAGCACTTCCGGCA
GTTGCGGCTTGCTCAGCTGCTCAAGGAGCTTGCATGGCAGCTTGTGCTGT
AGCTGCTCTAAATCTTGTCATCTAGATTATTGCGAAGAAAAAATTCGGTG
AAATATTATTGTCTCAAAGCAGAAGCTTTCAATCATTTCTCCATCTTTGA
CATTTACTCGTTTTCCTCATTTCTATATTAGTCAGTTTGCAAATCCTTTG
CAAATGGATGCTATTTTGTAAATAAATCTTTTTTACTTGTTTCTGTTACA
ATTTCGTAATAACGTTTTCTGTACCTTATTATGGAAATGTAACAGTTCTT
TGTGGAGTATATGCATAACTGTAATATTTATTATACTTTGCATATACTCT
TTTATATAGCGCTCGAGTTAGGAATATTTTTTTATACATCTTTTTAATAC
ATCTTTTTTTACATTTCTTGTTTCTTGTTCTTCGGCACCCATAGTTTTCC
TTCTATTGTCATTACCATCTCTTCTTTCAACATATATTTCCAACACGACC
CTTGCGCTACTTCATTTTTGATTTGTCTAATTTCCTTGTTGCCATTTACA
ACTTAGTATTTTCTACAACATGTTTAAACGCCATATTTTCCTTTTTTCTG
TCTTTGTATTCTCCTGACTCAGTAGGCATTGTGTACAAGTCCCAAAAGTA
CTTCTCGATAGTGCATCTACATTTGCATGATTCACTGTTTTTCTATACTT
AATTTTGATGTCAAAATACTGAGATAATTGATCTACCTTTGAAATTGCAC
AGTTATTGACTTTTTCGTAGATAACATAAAAGTAATTGCTTTGTATTCTG
TTCTCAATGTAAATATTTTTCCGTAAAAGTATTGATTAAAATGTTGGCAA
AAATAGTAAATTGCGAGCAGCTCCTTTCTCGTAATGCATTACCCTTTCTC
GTGACTACTTATTGCGTGTTACTCGTATGCTATTACCTTTTCGTAACCAT
TATTATCTCTTTGTAATAGTACAGCCCCAATTGTATCAAAACTAGCGTCT
GTATCTAGTATGAAATCTCTTGTAAAATCTGGATATCCCTAAACAGGTAA
TTTGGTAAATGCTTTCTTCATATTTTTAAAAGCCCTTTCACAGTTTTCTG
TCCGACGGATCAACGTCAATCATTTTAAAACTTAATTTTATGTCCGTTGA
TAATTATGCGTAAATATCCTCACAATCACTATTTTCACAAAACTTTATGC
ATATATTTAAGAAAATAGCGGTAAAATATTTTAACAATACTTTATTATCT
CATGCCAAATTTTTAAAGGTTGATGTAAAAAGTAATTACTTAAATATTAA
TTTCTCCATTACAGTTTTAAAAATAGATGAACTGAAACGCGTAAATGTCT
GAAAAAATGGCATTGGGGACTTTTATAAGTTTTTAATTTATGTATAAATG
CTTTTTGAACTAACACAAATATATATATCTATAATATTTTAGTGTCTCTT
TAATAGCTGTTTCTTCTATATCATGTTCGACACTCCACTAGAC

protein sequence of SMED30033630-orf-1

>SMED30033630-orf-1 ID=SMED30033630-orf-1|Name=SMED30033630-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=108bp
FHCQIMIRIAIVFIIVASSFNQINCGILGILGYGACQSVCNAGYVACLGS
LGLTAVKTLKILCNDSILYFTGALNAGAGALPAVAACSAAQGACMAACAV
AALNLVI*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000070	intestinal phagocyte
PLANA:0000419	prepharyngeal region

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Alignment
None	No IPR available	TMHMM	TMhelix	coord: 67..89
None	No IPR available	TMHMM	TMhelix	coord: 4..23
None	No IPR available	TMHMM	TMhelix	coord: 30..52

Main menu

SMED30033630

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 2

Owlery

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