SMED30033629
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30033629 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Alignments
SMED30033629 aligns in the following genomic locations:
Homology
BLAST of SMED30033629 vs. TrEMBL
Match: A0A0H3YJ76 (Slc17a-11 (Fragment) OS=Schmidtea mediterranea OX=79327 GN=slc17a-11 PE=2 SV=1) HSP 1 Score: 64.3142 bits (155), Expect = 5.696e-7 Identity = 67/298 (22.48%), Postives = 133/298 (44.63%), Query Frame = 1 Query: 373 PIFNWFGFPIMLKFHIKVCYFLLIHLNLFDHTMYNQIYELRQFYYQCENIAFKIPSRLVSLTSYKFVKYL-FDHHQIFKLFDIKKLNMSERILKSMDKFITALDKNASHRSIYGYVETCRSCGHKFSYLKEDFSIKL-IESFDYIINNK-FNYLIGCKKCYGSSQVEIKLERLADFLYIDCPRGVNKTFQELNEIILEKPSKNLKLQGIIELKCPDQFDDLPEKEPK----IEEFIMKNAVKPK------FEFRIHFRNTFGWHCVDTNSGISIDPISESDIKISPNKVKILVFAEIN 1227 I NW LK K+ FL++HL++F + + EL+ Y A LV+ + F+ L + K D + N +ILKS++ ++ ++ Y + CRSC +S L D+ + L + + + I+ K +Y I C+KC ++++I ++ ++ FL I G + + E + + L G++E K DD P + K ++++ A+ K ++ IH+R+ GW + N G+ + ++S + + L++ +++ Sbjct: 93 SIINWLKLS-TLKVQSKIICFLILHLDVFRLNSDSLLNELKDEYSIINKFANLCKMELVNSLCFNFISKLSLLSKSVSKNEDFDE-NFENQILKSLNNYLININSKERVLCKYTFHFDCRSCNVVYSKLHCDYVLTLPYDKYYFRISKKGISYRISCEKCSNRAEIKITVQNISKFLIIHNENGCRENIKRF-EAGFHLRNDSFPLIGVLESK-----DDFPRIDGKYQMNFQQYLTTIAISEKPAGSISDDYAIHYRHQNGWFTMSEN-GLPLSCPADSITTTAGLSARFLIYGKLS 381
BLAST of SMED30033629 vs. Planmine SMEST
Match: SMESG000068800.1 (SMESG000068800.1) HSP 1 Score: 1162.13 bits (3005), Expect = 0.000e+0 Identity = 608/615 (98.86%), Postives = 612/615 (99.51%), Query Frame = 1 Query: 43 MDLCPVCLENNKESSLINIGVHSKVLACKSEDCIYIEYEDINNTKPDSDLNYEINATEDFSDIECXXXXXXXXXXXXXXXXXXXXXXXXYQVLPIQRHLMLAIKDANKLTPIFNWFGFPIMLKFHIKVCYFLLIHLNLFDHTMYNQIYELRQFYYQCENIAFKIPSRLVSLTSYKFVKYLFDHHQIFKLFDIKKLNMSERILKSMDKFITALDKNASHRSIYGYVETCRSCGHKFSYLKEDFSIKLIESFDYIINNKFNYLIGCKKCYGSSQVEIKLERLADFLYIDCPRGVNKTFQELNEIILEKPSKNLKLQGIIELKCPDQFDDLPEKEPKIEEFIMKNAVKPKFEFRIHFRNTFGWHCVDTNSGISIDPISESDIKISPNKVKILVFAEINDPSCFRLSDLHLPIYKVLEQXXXXXXXXXXXXTESIKYWIILFKYRDHCLRKGDNSKSFLNKIATRITAKFNLHLKRRQNRIKEKFKGTQNEETFTSVRISDESESNELGETHNELMIEGNQDDKLESTIDFTELLVELKNNIPKERVDNIGNQWNNAESVEDGKKDIWSIFQQEDGPNIFNLLPKKSQPTRSAVREDDTETMIDQLISMKKKKRKLCEH 1887 MDLCPVCLENN+ESSLINIG+HSKVLACKSEDCIYIEYEDINNTKPDSDLNYEINATEDFSDIECDLSTTDSEDDNSSLSNKNKLKFSKYQVLPIQRHLMLAIKDANKLTPIFNWFGFPIMLKFHIKVCYFLLIHLN FDHTMYNQIYELRQFYYQCENIAFKIPSRLVSLTSYKFVKYLFDHHQIFKLFDIKKLNMSERILKSMDKFITALDKNASHRSIYGYVETCRSCGHKFSYLKEDFSIKLIESFDYIINNKFNYL GCKKCYGSSQVEIKLERLADFLYIDCPRGVNKTFQELNEIILEKPSKNLKLQGIIELKCPDQFDDLPEKEPKIEEFIMKNAVKPKFEFRIHFRNTFGWHCVDTNSGISIDPISESDIKISPNKVKILVFAEINDPSCFRLSDLHLPIYKVLEQIKKPPKPMIKYPTESIKYWIILFKYRDHCLRKGDNSKSFLNKIATRITAKFNLHLKRRQNRIKEKFKGTQNEETFTSVRISDESESNELGETHNELMIEGNQDDKLESTIDFTELLVELKNNIPKERVDNIG+QWNNAESVEDGKKDIWSIFQQEDGPNIFN+LP KSQPTRSAVREDDTETMIDQLISMKKKKRKLCEH Sbjct: 1 MDLCPVCLENNQESSLINIGIHSKVLACKSEDCIYIEYEDINNTKPDSDLNYEINATEDFSDIECDLSTTDSEDDNSSLSNKNKLKFSKYQVLPIQRHLMLAIKDANKLTPIFNWFGFPIMLKFHIKVCYFLLIHLNFFDHTMYNQIYELRQFYYQCENIAFKIPSRLVSLTSYKFVKYLFDHHQIFKLFDIKKLNMSERILKSMDKFITALDKNASHRSIYGYVETCRSCGHKFSYLKEDFSIKLIESFDYIINNKFNYLTGCKKCYGSSQVEIKLERLADFLYIDCPRGVNKTFQELNEIILEKPSKNLKLQGIIELKCPDQFDDLPEKEPKIEEFIMKNAVKPKFEFRIHFRNTFGWHCVDTNSGISIDPISESDIKISPNKVKILVFAEINDPSCFRLSDLHLPIYKVLEQIKKPPKPMIKYPTESIKYWIILFKYRDHCLRKGDNSKSFLNKIATRITAKFNLHLKRRQNRIKEKFKGTQNEETFTSVRISDESESNELGETHNELMIEGNQDDKLESTIDFTELLVELKNNIPKERVDNIGSQWNNAESVEDGKKDIWSIFQQEDGPNIFNILPNKSQPTRSAVREDDTETMIDQLISMKKKKRKLCEH 615
BLAST of SMED30033629 vs. Planmine SMEST
Match: SMESG000068800.1 (SMESG000068800.1) HSP 1 Score: 1122.07 bits (2901), Expect = 0.000e+0 Identity = 593/615 (96.42%), Postives = 597/615 (97.07%), Query Frame = 1 Query: 43 MDLCPVCLENNKESSLINIGVHSKVLACKSEDCIYIEYEDINNTKPDSDLNYEINATEDFSDIECXXXXXXXXXXXXXXXXXXXXXXXXYQVLPIQRHLMLAIKDANKLTPIFNWFGFPIMLKFHIKVCYFLLIHLNLFDHTMYNQIYELRQFYYQCENIAFKIPSRLVSLTSYKFVKYLFDHHQIFKLFDIKKLNMSERILKSMDKFITALDKNASHRSIYGYVETCRSCGHKFSYLKEDFSIKLIESFDYIINNKFNYLIGCKKCYGSSQVEIKLERLADFLYIDCPRGVNKTFQELNEIILEKPSKNLKLQGIIELKCPDQFDDLPEKEPKIEEFIMKNAVKPKFEFRIHFRNTFGWHCVDTNSGISIDPISESDIKISPNKVKILVFAEINDPSCFRLSDLHLPIYKVLEQXXXXXXXXXXXXTESIKYWIILFKYRDHCLRKGDNSKSFLNKIATRITAKFNLHLKRRQNRIKEKFKGTQNEETFTSVRISDESESNELGETHNELMIEGNQDDKLESTIDFTELLVELKNNIPKERVDNIGNQWNNAESVEDGKKDIWSIFQQEDGPNIFNLLPKKSQPTRSAVREDDTETMIDQLISMKKKKRKLCEH 1887 MDLCPVCLENN+ESSLINIG+HSKVLACKSED SDLNYEINATEDFSDIECDLSTTDSEDDNSSLSNKNKLKFSKYQVLPIQRHLMLAIKDANKLTPIFNWFGFPIMLKFHIKVCYFLLIHLN FDHTMYNQIYELRQFYYQCENIAFKIPSRLVSLTSYKFVKYLFDHHQIFKLFDIKKLNMSERILKSMDKFITALDKNASHRSIYGYVETCRSCGHKFSYLKEDFSIKLIESFDYIINNKFNYL GCKKCYGSSQVEIKLERLADFLYIDCPRGVNKTFQELNEIILEKPSKNLKLQGIIELKCPDQFDDLPEKEPKIEEFIMKNAVKPKFEFRIHFRNTFGWHCVDTNSGISIDPISESDIKISPNKVKILVFAEINDPSCFRLSDLHLPIYKVLEQIKKPPKPMIKYPTESIKYWIILFKYRDHCLRKGDNSKSFLNKIATRITAKFNLHLKRRQNRIKEKFKGTQNEETFTSVRISDESESNELGETHNELMIEGNQDDKLESTIDFTELLVELKNNIPKERVDNIG+QWNNAESVEDGKKDIWSIFQQEDGPNIFN+LP KSQPTRSAVREDDTETMIDQLISMKKKKRKLCEH Sbjct: 1 MDLCPVCLENNQESSLINIGIHSKVLACKSEDY--------------SDLNYEINATEDFSDIECDLSTTDSEDDNSSLSNKNKLKFSKYQVLPIQRHLMLAIKDANKLTPIFNWFGFPIMLKFHIKVCYFLLIHLNFFDHTMYNQIYELRQFYYQCENIAFKIPSRLVSLTSYKFVKYLFDHHQIFKLFDIKKLNMSERILKSMDKFITALDKNASHRSIYGYVETCRSCGHKFSYLKEDFSIKLIESFDYIINNKFNYLTGCKKCYGSSQVEIKLERLADFLYIDCPRGVNKTFQELNEIILEKPSKNLKLQGIIELKCPDQFDDLPEKEPKIEEFIMKNAVKPKFEFRIHFRNTFGWHCVDTNSGISIDPISESDIKISPNKVKILVFAEINDPSCFRLSDLHLPIYKVLEQIKKPPKPMIKYPTESIKYWIILFKYRDHCLRKGDNSKSFLNKIATRITAKFNLHLKRRQNRIKEKFKGTQNEETFTSVRISDESESNELGETHNELMIEGNQDDKLESTIDFTELLVELKNNIPKERVDNIGSQWNNAESVEDGKKDIWSIFQQEDGPNIFNILPNKSQPTRSAVREDDTETMIDQLISMKKKKRKLCEH 601
BLAST of SMED30033629 vs. Planmine SMEST
Match: SMESG000076754.1 (SMESG000076754.1) HSP 1 Score: 93.2041 bits (230), Expect = 1.804e-19 Identity = 75/305 (24.59%), Postives = 138/305 (45.25%), Query Frame = 1 Query: 376 IFNWFGFPIMLKFHIKVCYFLLIHLNLFDHTMYNQIYELRQFYYQCENIAFKIPSRLVSLTSYKFVKYLF-------DHHQIFKLFDIKKLNMSERILKSMDKFITALDKNASHRSIYGYVETCRSCGHKFSYLKEDFSIKLIESFDYIINNKFNYL---IGCKKCYGSSQVEIKLERLADFLYIDCPRGVNKTFQELNEIILEKPSKNLKLQGIIELKCPDQFDDLPEKEPKIEEFI-----MKNAVKPKFEFRIHFRNTFGWHCVDTNSGISIDPISESDIKISPNKVKILVFAEINDPSCFR 1245 +FNW P ++K H K+ L +HL++F I ++++ Y CE +A + P V Y+F+ L+ +H I K M ER++ + + D S + Y +C C +K S +++ + L + +++ K N L I C C + + I +R+ +F P+G+ + + I+ ++ L + GI+E F ++PE E++ M N +PK + +H RN GW+ ++ + +P E+ I N VK ++F I+DP +R Sbjct: 250 LFNWLRMPYLMKTHSKIVRILSLHLDVFKFKNNVFIDDIKREYSICETLANQFPMEKVRPVFYRFMNCLYGIIDKTPSNHLINK-------EMDERVIDLLLRLFEKYDSKGSLKLEYTTEFSCSFCDYKGSVNSKNYILSLKRN-RFLMKFKENQLIHNIKCLICKNALKETISFKRIPEFAIFHYPKGLREDISKYENKIITDDNRKLSIVGIVESSSEYPFAEVPENPISYLEYVFNDVTMDNQFRPK--YTVHVRNKRGWYSLNGYQQLLCNP-QEALGPIDFNHVKFIIFGVISDPQAYR 543
BLAST of SMED30033629 vs. Planmine SMEST
Match: SMESG000019961.1 (SMESG000019961.1) HSP 1 Score: 65.0846 bits (157), Expect = 1.028e-10 Identity = 67/297 (22.56%), Postives = 133/297 (44.78%), Query Frame = 1 Query: 376 IFNWFGFPIMLKFHIKVCYFLLIHLNLFDHTMYNQIYELRQFYYQCENIAFKIPSRLVSLTSYKFVKYL-FDHHQIFKLFDIKKLNMSERILKSMDKFITALDKNASHRSIYGYVETCRSCGHKFSYLKEDFSIKL-IESFDYIINNK-FNYLIGCKKCYGSSQVEIKLERLADFLYIDCPRGVNKTFQELNEIILEKPSKNLKLQGIIELKCPDQFDDLPEKEPK----IEEFIMKNAVKPKF------EFRIHFRNTFGWHCVDTNSGISIDPISESDIKISPNKVKILVFAEIN 1227 I NW LK K+ FL++HL++F + + + EL+ Y A LV+ + F+ L + K D + N +ILKS++ ++ + Y + CRSC +S L D+ + L + + + I+ K +Y I C+KC ++++I ++ + FL I G + + E + + L G++E K DD P+ + K ++++ A+ K ++ IH+R+ GW + N G+ + ++S + + L++ +++ Sbjct: 109 IINWLKLS-TLKVQSKIICFLILHLDVFRLNIDSLLNELKNEYSIINKFANLFKMELVNSLCFNFISKLSLLSKSVSKNEDFDE-NFENQILKSLNNYLINFNSKERVLCKYTFHFDCRSCNVVYSKLHCDYVLNLPYDKYYFRISKKGISYRISCEKCSNRAEIKITVQNIPKFLIIHNENGCRENIKRF-EAGFHLRNDSFPLIGVLESK-----DDFPQIDGKYQMNFQQYLTTIAISEKPAGSISDDYAIHYRHQNGWFTMSEN-GLPLSCPTDSITTTAGLSARFLIYGKLS 396
BLAST of SMED30033629 vs. Planmine SMEST
Match: SMESG000019961.1 (SMESG000019961.1) HSP 1 Score: 54.6842 bits (130), Expect = 1.269e-7 Identity = 61/258 (23.64%), Postives = 114/258 (44.19%), Query Frame = 1 Query: 373 PIFNWFGFPIMLKFHIKVCYFLLIHLNLFDHTMYNQIYELRQFYYQCENIAFKIPSRLVSLTSYKFVKYL-FDHHQIFKLFDIKKLNMSERILKSMDKFITALDKNASHRSIYGYVETCRSCGHKFSYLKEDFSIKL-IESFDYIINNK-FNYLIGCKKCYGSSQVEIKLERLADFLYIDCPRGVNKTFQELNEIILEKPSKNLKLQGIIELKCPDQFDDLPEKEPK----IEEFIMKNAVKPKF------EFRIHFR 1107 I NW LK K+ FL++HL++F + + + EL+ Y A LV+ + F+ L + K D + N +ILKS++ ++ + Y + CRSC +S L D+ + L + + + I+ K +Y I C+KC ++++I ++ + FL I G + + E + + L G++E K DD P+ + K ++++ A+ K ++ IH+R Sbjct: 108 SIINWLKLS-TLKVQSKIICFLILHLDVFRLNIDSLLNELKNEYSIINKFANLFKMELVNSLCFNFISKLSLLSKSVSKNEDFDE-NFENQILKSLNNYLINFNSKERVLCKYTFHFDCRSCNVVYSKLHCDYVLNLPYDKYYFRISKKGISYRISCEKCSNRAEIKITVQNIPKFLIIHNENGCRENIKRF-EAGFHLRNDSFPLIGVLESK-----DDFPQIDGKYQMNFQQYLTTIAISEKPAGSISDDYAIHYR 357 The following BLAST results are available for this feature:
BLAST of SMED30033629 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30033629 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30033629 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30033629 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30033629 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30033629 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30033629 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30033629 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of SMED30033629 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30033629 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30033629 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30033629 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30033629 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30033629 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30033629 ID=SMED30033629|Name=SMED30033629|organism=Schmidtea mediterranea sexual|type=transcript|length=1913bpback to top protein sequence of SMED30033629-orf-1 >SMED30033629-orf-1 ID=SMED30033629-orf-1|Name=SMED30033629-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=616bp MDLCPVCLENNKESSLINIGVHSKVLACKSEDCIYIEYEDINNTKPDSDLback to top Annotated Terms
The following terms have been associated with this transcript:
|