SMED30033603

Overview
NameSMED30033603
Smed IDSMED30033603
Length (bp)5065
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30033603 (SMED30033603) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30033603 (SMED30033603) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30033603 (SMED30033603) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30033603 (SMED30033603) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033603

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30033603 SmedASXL_075248SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30033603 SmedASXL_075248SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
testisSMED30033603 Contig6351newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30033603 vs. TrEMBL
Match: A0A087U6Z6 (Pogo transposable element with KRAB (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_09735 PE=4 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.581e-6
Identity = 39/105 (37.14%), Postives = 55/105 (52.38%), Query Frame = -2 
Query:  742 LTLYLQSLDIVNNKPCKDHI-*EINLFIEQK*ERNSRVNFIKPY*TMVCNVSDILWLELHQL**KIT*EILMLSL--------INLRRTHIYDHDRHGPLLKQEL 1029
            +T YLQ+LDI  NKP KDH+  EIN +IE +  RN R NF+KP    V N     W        KIT   +  +L         +   ++I  H+R GP+++QE+
Sbjct:  275 MTGYLQTLDIAINKPFKDHLRMEINEYIENRMVRNHRGNFVKPGLQEVVNWVKNSWD-------KITDSDVSNALRTGYLDKKYSFNDSYIARHERFGPMIQQEM 372

HSP 2 Score: 29.261 bits (64), Expect = 2.581e-6
Identity = 32/85 (37.65%), Postives = 43/85 (50.59%), Query Frame = -3 
Query: 1044 IRLVRKAAAPLVXXXXXXXXXXXXXXIFLMESAKAWSTQEAIK-----MLQFFDLGKR------DSASTYMTNE*KN----RPID 1253
            IRL  K  +P +I KG K +I   + I+++ES KAW TQ  I+     ML     G+       DSAST+   + KN    R ID
Sbjct:  183 IRLDGKKVSPFLITKGKKDQIQRVSGIYVIESEKAWCTQAVIRKWVDFMLPPLLRGQNRGLLVWDSASTHRAKDMKNFLAERKID 267
[Close]
BLAST of SMED30033603 vs. TrEMBL
Match: A0A087TJ39 (Pogo transposable element with KRAB (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_07293 PE=4 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.598e-6
Identity = 39/105 (37.14%), Postives = 55/105 (52.38%), Query Frame = -2 
Query:  742 LTLYLQSLDIVNNKPCKDHI-*EINLFIEQK*ERNSRVNFIKPY*TMVCNVSDILWLELHQL**KIT*EILMLSL--------INLRRTHIYDHDRHGPLLKQEL 1029
            +T YLQ+LDI  NKP KDH+  EIN +IE +  RN R NF+KP    V N     W        KIT   +  +L         +   ++I  H+R GP+++QE+
Sbjct:  307 MTGYLQTLDIAINKPFKDHLRMEINEYIENRMVRNHRGNFVKPGLQEVVNWVKNSWD-------KITDSDVSNALRTGYLDKKYSFNDSYIARHERFGPMIQQEM 404

HSP 2 Score: 29.261 bits (64), Expect = 2.598e-6
Identity = 32/85 (37.65%), Postives = 43/85 (50.59%), Query Frame = -3 
Query: 1044 IRLVRKAAAPLVXXXXXXXXXXXXXXIFLMESAKAWSTQEAIK-----MLQFFDLGKR------DSASTYMTNE*KN----RPID 1253
            IRL  K  +P +I KG K +I   + I+++ES KAW TQ  I+     ML     G+       DSAST+   + KN    R ID
Sbjct:  215 IRLDGKKVSPFLITKGKKDQIQRVSGIYVIESEKAWCTQAVIRKWVDFMLPPLLRGQNRGLLVWDSASTHRAKDMKNFLAERKID 299
[Close]
BLAST of SMED30033603 vs. TrEMBL
Match: A0A087T288 (Pogo transposable element with KRAB (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_23269 PE=4 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 2.906e-6
Identity = 39/105 (37.14%), Postives = 55/105 (52.38%), Query Frame = -2 
Query:  742 LTLYLQSLDIVNNKPCKDHI-*EINLFIEQK*ERNSRVNFIKPY*TMVCNVSDILWLELHQL**KIT*EILMLSL--------INLRRTHIYDHDRHGPLLKQEL 1029
            +T YLQ+LDI  NKP KDH+  EIN +IE +  RN R NF+KP    V N     W        KIT   +  +L         +   ++I  H+R GP+++QE+
Sbjct:  307 MTGYLQTLDIAINKPFKDHLRMEINEYIENRMVRNHRGNFVKPGLQEVVNWVKNSWD-------KITDSDVSNALRTGYLDKKYSFNDSYIARHERFGPMIQQEM 404

HSP 2 Score: 29.261 bits (64), Expect = 2.906e-6
Identity = 32/85 (37.65%), Postives = 43/85 (50.59%), Query Frame = -3 
Query: 1044 IRLVRKAAAPLVXXXXXXXXXXXXXXIFLMESAKAWSTQEAIK-----MLQFFDLGKR------DSASTYMTNE*KN----RPID 1253
            IRL  K  +P +I KG K +I   + I+++ES KAW TQ  I+     ML     G+       DSAST+   + KN    R ID
Sbjct:  215 IRLDGKKVSPFLITKGKKDQIQRVSGIYVIESEKAWCTQAVIRKWVDFMLPPLLRGQNRGLLVWDSASTHRAKDMKNFLAERKID 299
[Close]
BLAST of SMED30033603 vs. TrEMBL
Match: A0A087TYX9 (Pogo transposable element with KRAB (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_10749 PE=4 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 3.003e-6
Identity = 39/105 (37.14%), Postives = 55/105 (52.38%), Query Frame = -2 
Query:  742 LTLYLQSLDIVNNKPCKDHI-*EINLFIEQK*ERNSRVNFIKPY*TMVCNVSDILWLELHQL**KIT*EILMLSL--------INLRRTHIYDHDRHGPLLKQEL 1029
            +T YLQ+LDI  NKP KDH+  EIN +IE +  RN R NF+KP    V N     W        KIT   +  +L         +   ++I  H+R GP+++QE+
Sbjct:  307 MTGYLQTLDIAINKPFKDHLRMEINEYIENRMVRNHRGNFVKPGLQEVVNWVKNSWD-------KITDSDVSNALRTGYLDKKYSFNDSYIARHERFGPMIQQEM 404

HSP 2 Score: 29.261 bits (64), Expect = 3.003e-6
Identity = 32/85 (37.65%), Postives = 43/85 (50.59%), Query Frame = -3 
Query: 1044 IRLVRKAAAPLVXXXXXXXXXXXXXXIFLMESAKAWSTQEAIK-----MLQFFDLGKR------DSASTYMTNE*KN----RPID 1253
            IRL  K  +P +I KG K +I   + I+++ES KAW TQ  I+     ML     G+       DSAST+   + KN    R ID
Sbjct:  215 IRLDGKKVSPFLITKGKKDQIQRVSGIYVIESEKAWCTQAVIRKWVDFMLPPLLRGQNRGLLVWDSASTHRAKDMKNFLAERKID 299
[Close]
BLAST of SMED30033603 vs. TrEMBL
Match: A0A087TRQ1 (Pogo transposable element with KRAB (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_26215 PE=4 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 3.007e-6
Identity = 39/105 (37.14%), Postives = 55/105 (52.38%), Query Frame = -2 
Query:  742 LTLYLQSLDIVNNKPCKDHI-*EINLFIEQK*ERNSRVNFIKPY*TMVCNVSDILWLELHQL**KIT*EILMLSL--------INLRRTHIYDHDRHGPLLKQEL 1029
            +T YLQ+LDI  NKP KDH+  EIN +IE +  RN R NF+KP    V N     W        KIT   +  +L         +   ++I  H+R GP+++QE+
Sbjct:  307 MTGYLQTLDIAINKPFKDHLRMEINEYIENRMVRNHRGNFVKPGLQEVVNWVKNSWD-------KITDSDVSNALRTGYLDKKYSFNDSYIARHERFGPMIQQEM 404

HSP 2 Score: 29.261 bits (64), Expect = 3.007e-6
Identity = 32/85 (37.65%), Postives = 43/85 (50.59%), Query Frame = -3 
Query: 1044 IRLVRKAAAPLVXXXXXXXXXXXXXXIFLMESAKAWSTQEAIK-----MLQFFDLGKR------DSASTYMTNE*KN----RPID 1253
            IRL  K  +P +I KG K +I   + I+++ES KAW TQ  I+     ML     G+       DSAST+   + KN    R ID
Sbjct:  215 IRLDGKKVSPFLITKGKKDQIQRVSGIYVIESEKAWCTQAVIRKWVDFMLPPLLRGQNRGLLVWDSASTHRAKDMKNFLAERKID 299
[Close]
BLAST of SMED30033603 vs. Planmine SMEST
Match: SMESG000037576.1 (SMESG000037576.1)

HSP 1 Score: 728.398 bits (1879), Expect = 0.000e+0
Identity = 408/408 (100.00%), Postives = 408/408 (100.00%), Query Frame = 3 
Query: 3813 MIQYFYVIIELCFYLLNFIIILTECRISKSRIVKEISEMTFMLIILPFVTMSSTNKVRKLNVWLKFQTKIILILTMIGCLLISLKKIFSNIKNDYNKQINETNTHSCNQSMEXXXXXXXXXXXVFPLVVIDINYFIIAFILLINPIKNLDTHIEIFXXXXXXXXXXXXYVRFSILSWISLARFRIVTILLSIASMSVLFVCLFISSRSVPNFTGAHDIFLHLFTIYCMIGSFYGIFYYFVEVKIWLNMVENRNLKSDQYFNYVGTLATLLYTSVMIINLVSRIISERNVKRIVIDQLINYFCQTIQVLIQGFCIDSVFCKLIRNFTYSEVDSWSXXXXXXXXXXXXNLTTMAINVFYTQYPIFLNISHLFESSDYLWLILTNFCLPLTILHRFHAMTVFIHLTIKQWH 5036
            MIQYFYVIIELCFYLLNFIIILTECRISKSRIVKEISEMTFMLIILPFVTMSSTNKVRKLNVWLKFQTKIILILTMIGCLLISLKKIFSNIKNDYNKQINETNTHSCNQSMEIININKIIKSIVFPLVVIDINYFIIAFILLINPIKNLDTHIEIFSDDDDVNSSSKSYVRFSILSWISLARFRIVTILLSIASMSVLFVCLFISSRSVPNFTGAHDIFLHLFTIYCMIGSFYGIFYYFVEVKIWLNMVENRNLKSDQYFNYVGTLATLLYTSVMIINLVSRIISERNVKRIVIDQLINYFCQTIQVLIQGFCIDSVFCKLIRNFTYSEVDSWSFIIFPAFFLAFFNLTTMAINVFYTQYPIFLNISHLFESSDYLWLILTNFCLPLTILHRFHAMTVFIHLTIKQWH
Sbjct:    1 MIQYFYVIIELCFYLLNFIIILTECRISKSRIVKEISEMTFMLIILPFVTMSSTNKVRKLNVWLKFQTKIILILTMIGCLLISLKKIFSNIKNDYNKQINETNTHSCNQSMEIININKIIKSIVFPLVVIDINYFIIAFILLINPIKNLDTHIEIFSDDDDVNSSSKSYVRFSILSWISLARFRIVTILLSIASMSVLFVCLFISSRSVPNFTGAHDIFLHLFTIYCMIGSFYGIFYYFVEVKIWLNMVENRNLKSDQYFNYVGTLATLLYTSVMIINLVSRIISERNVKRIVIDQLINYFCQTIQVLIQGFCIDSVFCKLIRNFTYSEVDSWSFIIFPAFFLAFFNLTTMAINVFYTQYPIFLNISHLFESSDYLWLILTNFCLPLTILHRFHAMTVFIHLTIKQWH 408
[Close]
BLAST of SMED30033603 vs. Planmine SMEST
Match: SMESG000037573.1 (SMESG000037573.1)

HSP 1 Score: 254.988 bits (650), Expect = 2.992e-73
Identity = 180/518 (34.75%), Postives = 288/518 (55.60%), Query Frame = 3 
Query: 3507 SNYSGTENESSIFDNQSF----PVNWLCLAQDLSVSIILLFCVTMFMFETHELFCQSSKCSIREFSNINEGIYLLIVFSAIVTHMIQFRRNRNKWHREKFDNFTILMIQYFYVIIELCFYLLNFIIILTECRISKSRIVKEISEMTFMLIILPFVTMSSTNKVRKLNVWLKFQTKIILILTMIGCLLISLKKIFSNIKNDY-NKQINETN--THSCNQSMEXXXXXXXXXXXVFPLVVIDINYFIIAFILLINPIKNLDTHIEIFXXXXXXXXXXXXYVRFSILSWISLARFRIVTILLSIASMSVLFVCLFISSRSVPNFTGAHDIFLHLFTIYCMIGSFYGIFYYFVEVKIWLNMVENRNLKSDQYFNYVGTLATLLYTSVMIINLVSRIISERNVK-RIVIDQLINYFCQTIQVLIQGFCIDSVFCKLIRNFTYSEVDSWSXXXXXXXXXXXXNLTTMAINVFYTQYPIFLNISHL-FESSDYLWLILTNFCLPLTILHRFHAMTVFIHLTIKQW 5033
            ++Y   + + S  DN       P NW  +  ++ VS ++ F +   + E HE   ++    IR F+++ E  YL  V  +I+ H+ ++R NRN+W    + N+ ++++   Y ++E  +Y  NFI I T C    +++   + E  FM +ILPFV +S  N VRK + WLK +TK+ ++L+MIGC+LI  +KI   I  D  ++ +N T   ++SC+ S ++ +I+ I K  +  L + DINYFIIAFI LINP+  +DT  +   D  + +  S+   R S+  WISL RFR+ ++ LS   +S L V L+IS  S    T    I L+L +   +I   YGIFYY  ++  W   V NR+LK DQYFN++ + A ++++S M+++LV  +     V   I   +++N F  ++QVLIQ  CI S   +++R F   E+ SW  ++ P   LA FN   M  N  Y   P    +          +W+ + +  LP+ +L+RFHAM+ FI+LT+KQW
Sbjct:    2 NDYHDMDYQESDADNAEIFTVNPFNWSSVIVEICVSFLIAFAIFYVISEVHENSNKTDIEEIRSFNDVIETCYLSFVSLSIIFHIFRYRWNRNQWFDVMYGNYPMVIMMCIYALVETVYYTFNFISISTSC-FQLTKLFSSLGEFVFMGLILPFVLISIRNNVRKSHHWLKMRTKVTIMLSMIGCMLIFTRKILHKIGEDVISQSLNSTPVISNSCSSSGKLRDIHNICKLPLNILEIFDINYFIIAFIFLINPLLYIDTGED---DVTNRSIQSQHESRSSVFDWISLVRFRLFSVFLSFLIISTLLVTLYISVSSFD--TTIKTILLNLISFVYLIVIGYGIFYYLFDILYWDKEV-NRDLKIDQYFNHLTSSAQIIHSSTMLMSLVIVVFMNNKVDLEISYSEILNNFLLSVQVLIQASCIHSASERVVRRFVTGEIISWRSVLIPPLVLASFNFIMMITNFLYIFSPFLSRLPQTDINLGRIIWIFIKDLTLPIALLNRFHAMSFFINLTVKQW 512
[Close]
BLAST of SMED30033603 vs. Planmine SMEST
Match: SMESG000037637.1 (SMESG000037637.1)

HSP 1 Score: 190.274 bits (482), Expect = 4.514e-51
Identity = 160/494 (32.39%), Postives = 253/494 (51.21%), Query Frame = 3 
Query: 3597 VSIILLFCVTMFMFETHELFCQSSKCSIREFSNINEGIYLLIVFSAIVTHMIQFRRNRNKWHREKFDNFTILMIQYFYVIIELCFYLLNFIIILTECRISKSRIVKEISEMTFMLIILPFVTMSSTNKVRKLNVWLKFQTKIILILTMIGCLLISLKKIFSNIKNDYNKQINETNTHSCNQSMEXXXXXXXXXXXVFP-LVVIDINYFIIAFILLINPIKNL-------DTHIEIFXXXXXXXXXXXXYVRFSILSWISLARFRIVTILLSIASMSVLFVCLFISSRSVPNFTGAHDIFLHLFTIY---CM----IGSFYGIFYYFVEVKIWLNMVENRNLKSDQYFNYVGTLATLLYTSVMIINLVSRIISERNVKRIVIDQLINYFCQTIQVLIQGFCIDSVFCKLIRNFTYSEVDSWSXXXXXXXXXXXXNLTTMAINVFYTQYPIFLNISHLFESSDYLWLILTNFCLPLTILHRFHAMTVFIHLTIKQW 5033
            +S I+LF ++  + E  E+    +  SI  F+NI E I LL     I++++  FR  RNKW   K DN  +LMI   Y +IE+ FY + F+    +C+I     +  I+E+ FM  +LPF+ + +  KVR L   LK +TKI ++L+M+GC ++  +KI    K DY   +N T    C    E + + +     V   L+  +INYFII FILLINP  N+       + H EI  +            + S +  ISL RFR +T+LLS+  + +  V ++ +   + N        L ++ IY   C+    I ++Y I YYF ++  W         KSDQ FNY+  LA L + + + I   S I +        I QLI++     +++ Q  C+ S   ++    T +E  SW + + P   ++F+N  +M   V++  +P+ + IS   E   +  ++ T+F +PL ILHRFH MT FI+LT+KQW
Sbjct:   45 ISFIVLFMISYSIMEVEEIVHIDNLPSISVFNNILETIVLLATCICIISYIKTFRWKRNKWKPIKCDNVAMLMIMGIYGVIEMLFYTIIFLTYFIDCQIF-IYFISSIAELIFMFALLPFIVIVNLQKVRPLEKQLKLRTKITILLSMVGCFIVFSRKIGLRFKTDYQVLLNITFPVKCMYGSEFLEVVRQTSQSVQNVLLFFNINYFIIGFILLINPFLNINKEKITTENHTEILKE------------KTSFIERISLIRFRWLTVLLSLLILGITLVVVYFTVNILLNEKLELQTRLQMYFIYNVFCIILECITTYYNITYYFNDILKW-KFENGSRWKSDQSFNYLTALAQLCFLAGLTIENTSEIGTTN------IFQLIHFLSLIFEIMTQSVCVTSSCQRIAARHTENESHSWKYRLIPPLLISFYNCASMITLVYFLLHPVDIIISD--ERKTFTTIMWTDFIVPLCILHRFHGMTYFINLTMKQW 516
[Close]
BLAST of SMED30033603 vs. Planmine SMEST
Match: SMESG000069927.1 (SMESG000069927.1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.069e-13
Identity = 36/36 (100.00%), Postives = 36/36 (100.00%), Query Frame = 1 
Query: 1948 YIHNIRNIGKGVIADNGALAFPSILTVIIERENFQK 2055
            YIHNIRNIGKGVIADNGALAFPSILTVIIERENFQK
Sbjct:  501 YIHNIRNIGKGVIADNGALAFPSILTVIIERENFQK 536
The following BLAST results are available for this feature:
BLAST of SMED30033603 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033603 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033603 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033603 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033603 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033603 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033603 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033603 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A087U6Z62.581e-637.14Pogo transposable element with KRAB (Fragment) OS=... [more]
A0A087TJ392.598e-637.14Pogo transposable element with KRAB (Fragment) OS=... [more]
A0A087T2882.906e-637.14Pogo transposable element with KRAB (Fragment) OS=... [more]
A0A087TYX93.003e-637.14Pogo transposable element with KRAB (Fragment) OS=... [more]
A0A087TRQ13.007e-637.14Pogo transposable element with KRAB (Fragment) OS=... [more]
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BLAST of SMED30033603 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033603 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033603 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033603 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033603 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033603 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000037576.10.000e+0100.00SMESG000037576.1[more]
SMESG000037573.12.992e-7334.75SMESG000037573.1[more]
SMESG000037637.14.514e-5132.39SMESG000037637.1[more]
SMESG000069927.11.069e-13100.00SMESG000069927.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033603 ID=SMED30033603|Name=SMED30033603|organism=Schmidtea mediterranea sexual|type=transcript|length=5065bp
TTGTATTCTGTTATAGGCTTATTGTGTACACGTGTAGTGAGTGTGCTTAG
ATTTTATTATTCTTCCTTTCTATTGTAAATGCCCCGAATTTATGTCGGTG
TCCATAATTTTAGTCCTTATTTCAGGTAGGAGTGTGTTTATTTTTTCTAA
ATATCAATTAATTACATTTTATGTAACATTTTAATTTGTAGCAAAGCTGA
CCGTAATTTTTGTGCTAAATATTTAAATTTTTTTTTCGTTGAAATGGTTT
AAAATTGATTGAAATAAATTGTTTTATTAAATTGAACGTAGTCTGGAGGA
GCGACAGGATACAATTAAAGAAAGAGTACATTGTGTCTTGATCTTACAAT
TGAGGGTGATACTCTTTGGTTTATACCCATTTGATTTAACTGAATTGTGA
ATTATGGAAATTTTTTAAATTTGGAAAACAAAGATTTTGTTGAGATTGAC
TACTTTGATTTTATTGATTGTGAACAGATTGAGTTTCAATTCCATCGACA
AATTTCGTACAACCCAACAATTTTATATTAATATAGTTGTCCTCTTCTTC
TCGTTTGCTATCCATGCATAACTGTTCCTCCACAAAACATCAATTTTAAG
TTTTTACTATCTTTTTTAATTAATTTTTGTATTTATTTACCCCTTTTATT
GACCCAAAATAGAGACTATTTAAAAACAAAGAAAAATTTCTAATTTTTTT
TATTCACCAGCAACACTGAAGGTATCATCAATGTTCTTGATTAATTCTTG
CTTAAGCAGAGGCCCGTGTCGATCATGGTCGTAAATATGTGTCCTTCGAA
GATTTATTAAGGAAAGCATAAGTATTTCTCATGTAATTTTCTATCATAAT
TGATGTAACTCGAGCCAAAGAATGTCGGAAACATTGCAAACCATCGTCTA
ATAAGGTTTTATAAAGTTTACTCTTGAATTTCGCTCCTATTTTTGTTCAA
TAAAAAGATTTATTTCTCAAATATGATCTTTACATGGTTTATTATTAACA
ATGTCCAAACTTTGCAAATATAGAGTTAGTACTCGAGTTTACCATCAATT
GGTCGATTCTTTCATTCATTAGTCATGTACGTGCTTGCAGAATCCCTTTT
GCCTAAATCGAAGAACTGGAGCATTTTAATTGCCTCCTGTGTACTCCAAG
CTTTTGCCGATTCCATTAAAAATATACTGGTTTTCTCAATGATTTCGATT
TTTGAACCTTTAATGATAACCAAGGGAGCTGCAGCTTTCCTAACCAACCG
AATGAATGTAAACTGAACTACTTGCTCTGTGGATAAGCTCTATTCTTCAT
TGTAGTAAAATGATTTTGAGTTGCTTAATAATGTGATTTAATGTGACTGA
TTTAAAACCACAATGAGCTGTAGCGCTTTCTGGGCCAATGCGACAGCAGC
TATTCACTACGAGTTTTCCTCATTATCTACATTACAACAATTCAAAACCG
AATATTGAATTATGTTTATAAACCTAGCGTGCTGTGTGTCATCCGCCGAA
TCTATGATCTGTATTCAGATTCTGAGCCAATAGAAGCCGATCATTATAAC
CGAACGGTTCAGTTCTGCCATAATGTGGTCTCTAAGGGTTTCCCCAACCG
CAGAAGATCAAACTGGGCCAAACACAAGGACTGTGCGTGCTTCCCCGGCA
ACAATTTCTGGGTTTGTACCACTAACGGCACTCTTAACATATTCCCATTA
AATGACCAAAAATCTTGGGCCAAATCATCCAAATCGCCATGGCAACAACC
ATAATGCATTTGCAACAAAGATAATCTGATCTGGAAGCAATTTTTTTTAG
ATGAAATAGAGAAACGTGTTTGCATTTTGATTGACAATGAAACCGAACGC
AATGAGCAAAAGGCTAAATAGTTTGAATCAAAATTAGAAAATGAATACAA
ATCTTTGGAGCCAGTAGATTGGTGTTCGATCAATGGTAAAAAGAAGTTAT
ATCCACAATATTCGAAACATTGGAAAAGGAGTGATTGCAGATAATGGTGC
TTTAGCTTTTCCATCAATCTTAACAGTCATAATTGAAAGAGAAAATTTTC
AGAAAAGTTAATAAAATTTATTTCATGATAGAATTAACAGTAGTAGTTGT
TAGGAAATTAATTGAAAGAAATCCAGATGATAATTAATTATCGTTTAATT
TCCAGATGGCAGTTGTAGGATTTATTAGAAGATTGTCAAAAGAGAGATTC
TTTCAATTAATTAGCTATATTTATTACAAAATTAAAATCAGAAATGTGGC
TTTTAGTTTGAGTTTATAAGCAAATAGAAACTGGCAGTAAGACTAAAAAG
AATTTATTGTCAAAATAATTATCAGGAAAATCTTTGGAAAAGCACAGCAA
ATATGTATAACATGAAAATTAGTAAAAATATTGTAAAGTTTACTTAAAAT
TTGGTATTTATCAATTTATTTATTTTTTATTTATTAGTTTTATATTCGTC
ATACACCAGCTTTCATAAATTATATGCAACAAAGAAAAATATTATGGATA
ATTAATTATAACAATTGATTTGTAATTTGATTTTAATTTGTTAAGTTAAC
AATTATCCTTATTGAAATATATTTTTTTTCTCGAAACTATAAATTTAGTT
TTATATATTTATTGATGTTTTCAGGAATTTATAAATTTTTTTAAAAAGTT
AACATTCCTTTATCAATGTATGATTAAAAGCTAATTGCTTTCAATAAGTA
ATCACGATAATATTTTATATTTTATAAATATGATAATACGACAATCATTT
CAAACAAGTAAGTAATAAGATAATCCCTTCACGCAAAGTATATATTAAAT
GAAAAAAAAATCGATTATAAAATAATCACTATCAATTGATTTGCAGCAAG
GGCTTATATTATAATTTGCTGGTTTGAAAATTTAATTTATGCTGCTACCA
AGTAATCACCGCTTTATACTGAACTCATTAGTTACCGTACATTTGAACTA
AAATTATTTAAATCGCGTTATAATGTCCTATCCATTGTTTCATAACAATT
TCCCCTAAATCTATTTTTAATTTATCCGAATTAAATGAAACTAGTAACAC
ATTCATCTATAACATATTTAGTAAAGATGGTGTTATTGGTACATAATAGT
CAACGAGTTAAAGTTGCGTCTTTATAGGACAAAATACTTTCATTTTCATA
GTAAATTTAACAAAGTTTTTAAATAAAATAGCTGGAGTGATTTTTGATAT
TAAATAAAAAAATATTAGATTTTTATGAATATTGTAAGTGCAAATATGCT
TCAATATAAAATTAATATAAAATAAATTTGGTCCTAGAAAATAAATAATA
TTTAATTAAAAATTATGGATTATAATTATAGTATAAACAAAAAAATTAAG
CATAAAAGTTGATTATGTATTATTTATAATATTTTCTCATTTATTAAGAA
TTCAAAGGTCAATTTTCTAGAATTTCTATAAATAAAAATAAAATAAATAT
CAACCTGAATGCTGTAAAAATATTCGATTATGACTGAATTATTATGCGAT
ACCGATTCGAATTATTCTGGAACCGAAAATGAATCTAGCATATTTGACAA
TCAATCATTTCCCGTCAACTGGTTATGCTTGGCGCAAGATTTAAGCGTGA
GCATTATACTTTTATTTTGCGTAACAATGTTTATGTTTGAAACTCATGAA
TTGTTTTGCCAATCTTCAAAGTGTTCGATCAGAGAATTCAGTAACATTAA
CGAAGGTATTTATTTACTAATAGTATTTTCTGCCATTGTCACCCATATGA
TCCAATTCAGACGAAATAGAAACAAATGGCATCGGGAAAAGTTTGACAAT
TTCACAATATTAATGATTCAATATTTCTACGTCATAATTGAATTATGCTT
TTATCTTTTGAATTTCATAATCATACTTACCGAATGTAGAATCAGCAAAA
GTCGAATCGTTAAAGAAATATCGGAAATGACATTCATGTTGATCATCTTA
CCGTTTGTGACGATGAGTTCCACAAATAAAGTGAGAAAATTGAATGTTTG
GTTGAAATTCCAGACGAAAATTATTTTAATTCTGACTATGATCGGCTGTT
TGTTGATTTCATTGAAAAAAATATTTTCCAATATCAAAAACGATTACAAT
AAACAGATTAATGAAACCAACACTCACAGTTGCAATCAATCTATGGAAAT
TATAAACATAAATAAAATCATCAAATCGATTGTGTTCCCATTAGTGGTCA
TAGATATCAACTATTTCATCATTGCTTTTATTTTATTGATCAATCCCATA
AAAAATCTTGACACACACATTGAGATTTTTAGTGATGATGATGACGTCAA
TTCATCCAGCAAATCATATGTTCGCTTTTCAATTTTGAGTTGGATTTCAT
TGGCCAGATTTCGAATAGTAACAATTCTATTATCAATCGCTAGTATGAGC
GTGTTATTTGTCTGCTTATTTATTTCATCAAGAAGCGTTCCGAATTTCAC
CGGAGCACATGATATATTTTTGCATTTGTTTACGATATACTGTATGATTG
GTTCATTTTATGGAATATTCTACTATTTTGTTGAAGTGAAAATATGGTTG
AATATGGTTGAAAATCGAAACCTGAAAAGCGATCAATATTTTAACTATGT
AGGAACTTTAGCGACTTTATTATATACATCGGTTATGATAATCAATCTTG
TTAGTAGAATAATTAGTGAAAGAAATGTAAAACGCATTGTAATAGATCAA
CTGATTAACTATTTTTGCCAAACTATTCAAGTTCTTATTCAAGGATTCTG
TATTGACTCTGTGTTTTGCAAGCTGATTAGAAATTTCACCTACTCAGAAG
TTGACAGTTGGAGCTTCATTATTTTCCCGGCATTTTTTCTGGCTTTCTTT
AACCTGACAACAATGGCAATAAATGTATTTTATACGCAATATCCAATTTT
TTTGAATATTTCTCATTTGTTTGAATCTAGCGATTATTTGTGGTTGATTT
TGACAAATTTCTGTTTGCCATTGACAATTTTACATAGATTTCATGCAATG
ACAGTTTTTATTCATTTAACAATAAAGCAGTGGCATTAAATACACAAATT
TTTTTGACAAAAAAA
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protein sequence of SMED30033603-orf-1

>SMED30033603-orf-1 ID=SMED30033603-orf-1|Name=SMED30033603-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=520bp
MTELLCDTDSNYSGTENESSIFDNQSFPVNWLCLAQDLSVSIILLFCVTM
FMFETHELFCQSSKCSIREFSNINEGIYLLIVFSAIVTHMIQFRRNRNKW
HREKFDNFTILMIQYFYVIIELCFYLLNFIIILTECRISKSRIVKEISEM
TFMLIILPFVTMSSTNKVRKLNVWLKFQTKIILILTMIGCLLISLKKIFS
NIKNDYNKQINETNTHSCNQSMEIININKIIKSIVFPLVVIDINYFIIAF
ILLINPIKNLDTHIEIFSDDDDVNSSSKSYVRFSILSWISLARFRIVTIL
LSIASMSVLFVCLFISSRSVPNFTGAHDIFLHLFTIYCMIGSFYGIFYYF
VEVKIWLNMVENRNLKSDQYFNYVGTLATLLYTSVMIINLVSRIISERNV
KRIVIDQLINYFCQTIQVLIQGFCIDSVFCKLIRNFTYSEVDSWSFIIFP
AFFLAFFNLTTMAINVFYTQYPIFLNISHLFESSDYLWLILTNFCLPLTI
LHRFHAMTVFIHLTIKQWH*
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protein sequence of SMED30033603-orf-2

>SMED30033603-orf-2 ID=SMED30033603-orf-2|Name=SMED30033603-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=152bp
MEKLKHHYLQSLLFQCFEYCGYNFFLPLIEHQSTGSKDLYSFSNFDSNYL
AFCSLRSVSLSIKMQTRFSISSKKNCFQIRLSLLQMHYGCCHGDLDDLAQ
DFWSFNGNMLRVPLVVQTQKLLPGKHAQSLCLAQFDLLRLGKPLETTLWQ
N*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0002109X1 cell
PLANA:0002111X2 cell
PLANA:0003116parenchymal cell