SMED30033573

Overview
NameSMED30033573
Smed IDSMED30033573
Length (bp)5200
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30033573 (SMED30033573) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30033573 (SMED30033573) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033573

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30033573SMESG000070092.1 dd_Smed_v4_50850_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30033573SMESG000070092.1 dd_Smed_v4_50850_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30033573 vs. TrEMBL
Match: Q91S73 (PASVE_E1 OS=Planaria asexual strain-specific virus-like element type 1 OX=159252 GN=PASVE_e1 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 2.608e-25
Identity = 58/157 (36.94%), Postives = 92/157 (58.60%), Query Frame = -1 
Query: 4103 RYSKIRIIQTIMELRGGKINTIYLQGNILYDNLFYSIGPSNTRKTFFVQLLTVDMKVGCIHNMNNKNTFWTQDVDGKDVVVAEELMLNMENVDYFKLMLEGSNMIRIEIKNKPAVNWKRIPVIVTSNQWIWQFVSNHQEALQNRMLVIKFNQPIDFG 4573
            +Y  + II +I+E++ GK N I +            +G  N  K+ F  +L   +K G  H+MN+++ FW QD+ GKD+ V EE  +++ N +YFK +LEGS  + +E KN P+V+ +R PVI T N++ W+ VS+  EA + R L+I  +  I F 
Sbjct:   58 KYKTMAII-SILEMQAGKTNVILM------------VGKPNCGKSLFCNVLCAGLKTGAAHSMNSRSNFWLQDLQGKDIYVFEEFSMDVFNANYFKALLEGSMHVMVEKKNMPSVHLERRPVITTGNEFPWKLVSSTAEAFRERSLIILTDSQIPFS 201
[Close]
BLAST of SMED30033573 vs. TrEMBL
Match: A0A2Z4QAE6 (Putative viral replication protein OS=CRESS virus sp. OX=2202563 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.726e-13
Identity = 56/170 (32.94%), Postives = 87/170 (51.18%), Query Frame = -2 
Query:   31 GDIDREFDYASKEATRVTDGLILSIGTPPKTLPQLKDIFRQLLTFDNPDDGMTFLREKDPQTYVLQGQKIESCL--VRESRKNMSSKYVKFNIPFVNVRD-GKTHVFV-GSTGIGKTNFALAHFRRAILCSDRQDFGKISTSTDGLVIDDMTFKSW-LPTNLHICDVAFD 525
            G + +  DY SK+ + V      S G+P        +IFR  +  ++ D  +  +R++ P+ YVL   +I + L  + +  +  S +  KF++ F       K  VF+ G +G GKT FALAHF R +L     D  +I+  TDG+V DDM+F  W   T +HI D+  D
Sbjct:   91 GTVKQSSDYCSKDESCVDPDTRFSYGSPVSVSETKDEIFRSAIYAESYDAAIEIIRDRAPRDYVLSRDRIHAGLKEIHQGVRENSYRLFKFSMAFFTTEILEKYSVFLHGFSGAGKTAFALAHFERPVLIRHIDDAKRITRGTDGVVFDDMSFTHWPRVTCIHILDLEHD 260
[Close]
BLAST of SMED30033573 vs. TrEMBL
Match: Q77I17 (Rep OS=Planaria asexual strain-specific virus-like element type 1 OX=159252 GN=rep PE=4 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 2.506e-13
Identity = 53/204 (25.98%), Postives = 100/204 (49.02%), Query Frame = -2 
Query:   67 MSERGHHHCEVFLHTGNNMSVRALNTALGIHYSWGFSAGDIDREFDYASKEATRVTDGLILSIGTPPKTLPQLKDIFRQLLTFDNPDDGMTFLREKDPQTYVLQGQKIESCLVRESRKNMSSKYVK-FNIPFVN--VRDGKT-------HVFVGSTGIGKTNFALAHFRRAILCSDRQDFGKISTST---DGLVIDDMTFKSWL 639
            MS  G  H ++F+      +   +    G+ +     + D+ +  DY +K  TRV +G+ +  G   + L Q ++ + + L  ++  +GM  +  + P  Y+   +K+E  L+ +      +K+   +  P+     RD +T        VFVG +GIGKTNFAL+HF + +  +++Q+  K+ ++    DG V+DD+    W 
Sbjct:    1 MSSSGFRHIQLFITCKYATTASNVAKKFGVGFVGVVESHDVQKWVDYCTKVDTRVENGVTIKHGERAR-LSQDQEFWAKFLNVNSEREGMEMVERERPLDYIRFREKLEGALLHKLGSVTQAKFKSGWRWPYYGFMYRDPETDEVSMRSWVFVGKSGIGKTNFALSHFEQPLHVTEKQELRKLGSTKFPYDGFVLDDVHLAEWF 203
[Close]
BLAST of SMED30033573 vs. TrEMBL
Match: Q910M2 (Rep OS=Planaria asexual strain-specific virus-like element type 2 OX=159253 GN=rep PE=4 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 2.506e-13
Identity = 53/204 (25.98%), Postives = 100/204 (49.02%), Query Frame = -2 
Query:   67 MSERGHHHCEVFLHTGNNMSVRALNTALGIHYSWGFSAGDIDREFDYASKEATRVTDGLILSIGTPPKTLPQLKDIFRQLLTFDNPDDGMTFLREKDPQTYVLQGQKIESCLVRESRKNMSSKYVK-FNIPFVN--VRDGKT-------HVFVGSTGIGKTNFALAHFRRAILCSDRQDFGKISTST---DGLVIDDMTFKSWL 639
            MS  G  H ++F+      +   +    G+ +     + D+ +  DY +K  TRV +G+ +  G   + L Q ++ + + L  ++  +GM  +  + P  Y+   +K+E  L+ +      +K+   +  P+     RD +T        VFVG +GIGKTNFAL+HF + +  +++Q+  K+ ++    DG V+DD+    W 
Sbjct:    1 MSSSGFRHIQLFITCKYATTASNVAKKFGVGFVGVVESHDVQKWVDYCTKVDTRVENGVTIKHGERAR-LSQDQEFWAKFLNVNSEREGMEMVERERPLDYIRFREKLEGALLHKLGSVTQAKFKSGWRWPYYGFMYRDPETDEVSMRSWVFVGKSGIGKTNFALSHFEQPLHVTEKQELRKLGSTKFPYDGFVLDDVHLAEWF 203
[Close]
BLAST of SMED30033573 vs. TrEMBL
Match: A0A5Q2F4S9 (Replication associated protein OS=Antarctic virus COCH21_51 OX=2664243 GN=rep PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.964e-10
Identity = 47/166 (28.31%), Postives = 88/166 (53.01%), Query Frame = -2 
Query:   34 DYASKEATRVT-DGLILSIGTPPKTLPQLKDI---FRQLLTFDNPDDGMTFLREKDPQTYVLQGQKIESCL--VRESRKNMSSKYVKFNI-PFVN-VRDGKTHVFVGSTGIGKTNFALAHFRRAILCSDRQDFGKISTSTDGLVIDDMTFKSWLPTN-LHICDVAF 504
            +Y  KE TR+   G     G   + L + K     +++ L   +  D +  ++++ P+ YV+   ++ S L  + +   N +   V FN+ PF     + +  +F+G +G+GKT FA++HF+  ++ S   D  K++ + DG++ DDM F+ W PT  +H+CD+  
Sbjct:   82 EYCKKEETRMQGPGTSFEFGNVMEELKKRKSPDSPYKKALNCLSYADSIAIIKDEAPRDYVIYHNQVTSTLKAIFKPVWNKTPGLV-FNLEPFPEETLNTRAVIFMGRSGLGKTQFAISHFKEPLIVSHIDDLKKLNPNIDGIIFDDMNFQHWPPTACIHLCDMEL 246
[Close]
BLAST of SMED30033573 vs. Planmine SMEST
Match: SMESG000059230.1 (SMESG000059230.1)

HSP 1 Score: 196.438 bits (498), Expect = 2.019e-119
Identity = 104/179 (58.10%), Postives = 120/179 (67.04%), Query Frame = -2 
Query: 2914 MPVRHREGRWGQVDDFTMVDVDNALRRMYHSKYSDNKRWYYKNTKWDTRKYPSQFRAPAINGWFGIGDEHQVWDFKTKKWISQIKK*QPNQYLHRNWDNYAYENQYYKSSEHNFYWSLNSEDWKKYKEVIYXXX-----XXXXXXXXPRTKGYWGPGFKKDIENCGGYRPDIYRAWCDA 3435
            MP+RHRE RWG V DFTM+DVDNALRRMYHSKYS+  RWY     +D RK  S+ R P  N W GIGDE Q+WD KT+KWISQ  K   +QYL  N+  Y     Y+K SE+N YWSL  E+WKKYK+++YKVG                K YWG GFKKD EN GGYRPDIYRAWC+A
Sbjct:  353 MPLRHREDRWGAVQDFTMLDVDNALRRMYHSKYSNTDRWYTPVMYFDYRK--SKLRDPMTNSWNGIGDERQIWDHKTRKWISQYSKDGNHQYLDTNYSKYIQTYNYHKQSEYNLYWSLTKEEWKKYKQIVYKVGPPPAWAMIEDKNDNNKKTYWGNGFKKDGENWGGYRPDIYRAWCEA 529

HSP 2 Score: 124.02 bits (310), Expect = 2.019e-119
Identity = 66/94 (70.21%), Postives = 78/94 (82.98%), Query Frame = -3 
Query: 3465 ASKALRKNNPIDRWRHEATIKKLKENLKEIDPYNDVLDKHNKWLMDKPERHSQSMQHLLDLKAKGGNFIKKMMPFIKNSPKTXXXXXXXXLIDF 3746
            A K   KNNPIDRWRHE  IKKL+ +LKE++PY DVLDKHNKWL  KPER +QSM +LL+LKAKGGNF KKM+PF+K + K+L KLGL+GLIDF
Sbjct:  236 AKKGFEKNNPIDRWRHEKAIKKLETSLKELEPYGDVLDKHNKWLNAKPERRTQSMAYLLELKAKGGNFFKKMLPFLKKAGKSLSKLGLIGLIDF 329

HSP 3 Score: 100.908 bits (250), Expect = 2.019e-119
Identity = 48/65 (73.85%), Postives = 54/65 (83.08%), Query Frame = -1 
Query: 3878 VSKDLLYRHTQDMDIKIKILQSAQQRDMDRGLTFKGDQRKVYDKLMGDLNKQKYVPTPAETKELR 4072
            VSK L+YRHTQD DIK+KILQSAQQRD+DRGLTF GDQRK Y KL+GDL   KY+P PAE  EL+
Sbjct:  126 VSKSLIYRHTQDTDIKMKILQSAQQRDIDRGLTFTGDQRKEYTKLLGDLKNSKYIPDPAEMIELQ 190

HSP 4 Score: 73.1738 bits (178), Expect = 2.019e-119
Identity = 34/50 (68.00%), Postives = 44/50 (88.00%), Query Frame = -2 
Query: 3748 QKLKNSEHDKQVKKQLDNEMNQARKMKKFKPTNNIKTMFDIREYYRH*LD 3897
            + ++  E+DK++K +L++EMN+ARKMKKFKPTNNIKTMFDIRE YRH LD
Sbjct:  185 EMIELQEYDKKMKLELEDEMNKARKMKKFKPTNNIKTMFDIRELYRHELD 234

HSP 5 Score: 243.817 bits (621), Expect = 5.329e-66
Identity = 114/176 (64.77%), Postives = 138/176 (78.41%), Query Frame = -2 
Query:   31 SWGFSAGDIDREFDYASKEA----TRVTDGLILSIGTPPKTLPQLKDIFRQLLTFDNPDDGMTFLREKDPQTYVLQGQKIESCLVRESRKNMSSKYVKFNIPFVNVRDGKTHVFVGSTGIGKTNFALAHFRRAILCSDRQDFGKISTSTDGLVIDDMTFKSWLPTN-LHICDVAFD 543
            +WG    DI R +  A        TRV  G++LSIGTPPKTLP  KDI+RQLL  DNP++ M FL+EKDPQTY+LQ QKIE+CL+RE+R+N+++K+  FN PF++V D KTH+F+G TGIGKTNFALAHF++ ILCSDRQDFGKI  STDGLVIDDMTF+SWLPTN LHI D  FD
Sbjct:  514 NWGGYRPDIYRAWCEAKTHPDGYTTRVDGGVVLSIGTPPKTLPHTKDIYRQLLQIDNPEEAMEFLKEKDPQTYILQRQKIEACLIREARRNVNAKFTSFNRPFIDVNDKKTHIFIGGTGIGKTNFALAHFKKPILCSDRQDFGKIGASTDGLVIDDMTFRSWLPTNLLHIVDCEFD 689
[Close]
BLAST of SMED30033573 vs. Planmine SMEST
Match: SMESG000070092.1 (SMESG000070092.1)

HSP 1 Score: 321.242 bits (822), Expect = 5.390e-101
Identity = 151/157 (96.18%), Postives = 153/157 (97.45%), Query Frame = -2 
Query: 1750 MMGEPIEPQKVQKRGTGHREFVINGTIVLSNTGADANTSWFPFHWEFMLSWIVGTQNFKDLKTFLYYKSKTASVLFYNPIATYQTADTDMKGLNPEAKILGFVDKLHMYNPVTGPSHDQGWEYYDTMFANNALRCGGMDTKTGSFKIGKTVNLAKSL 2220
            MMGEPIEPQKVQKRGTGHREFVINGTIVLSNTGADANTSWFPFHWEFML WIVGTQNFKDLKTFLYYKSKTASVLFYNPIATYQTADTDMK LNPEAKILGFVDKLHMYNPVTGPSHDQGWEYYDTMFANNALRCGGMDTKTGSFKIGKTVN + +L
Sbjct:    1 MMGEPIEPQKVQKRGTGHREFVINGTIVLSNTGADANTSWFPFHWEFMLPWIVGTQNFKDLKTFLYYKSKTASVLFYNPIATYQTADTDMKSLNPEAKILGFVDKLHMYNPVTGPSHDQGWEYYDTMFANNALRCGGMDTKTGSFKIGKTVNPSTTL 157
[Close]
BLAST of SMED30033573 vs. Planmine SMEST
Match: SMESG000059232.1 (SMESG000059232.1)

HSP 1 Score: 248.44 bits (633), Expect = 9.058e-84
Identity = 111/139 (79.86%), Postives = 122/139 (87.77%), Query Frame = -2 
Query:  229 EEMSERGHHHCEVFLHTGNNMSVRALNTALGIHYSWGFSAGDIDREFDYASKEATRVTDGLILSIGTPPKTLPQLKDIFRQLLTFDNPDDGMTFLREKDPQTYVLQGQKIESCLVRESRKNMSSKYVKFNIPFVNVRDG 645
            +EMSERGHHHCEVFLHTGNNMSVRALN ALGI YSWG  A + DREFDY SKE TRV DG+ILSI TPPKTLPQ KD++RQLL FDNPDDGM +L+EKDPQTYVLQ QKIESCL+ ESRK MS+KY KFN+PF+NVRDG
Sbjct:   57 DEMSERGHHHCEVFLHTGNNMSVRALNNALGIQYSWGVPAAEFDREFDYCSKETTRVADGIILSIETPPKTLPQSKDLYRQLLAFDNPDDGMEYLKEKDPQTYVLQRQKIESCLIHESRKTMSAKYSKFNVPFINVRDG 195

HSP 2 Score: 84.3445 bits (207), Expect = 9.058e-84
Identity = 43/59 (72.88%), Postives = 47/59 (79.66%), Query Frame = -3 
Query:  702 MSNSNNYHYDENSDRPP-PIVQLGRFRNFRFSIPFQMYMDNRVTILEFANTRCKFFYLQ 875
            MSNS N    EN++R   PIVQLGRFRNFRFSIPFQ Y+D +  ILEFANTRCKFFYLQ
Sbjct:    1 MSNSTN---GENTERHSVPIVQLGRFRNFRFSIPFQTYVDKKAYILEFANTRCKFFYLQ 56
[Close]
BLAST of SMED30033573 vs. Planmine SMEST
Match: SMESG000059229.1 (SMESG000059229.1)

HSP 1 Score: 112.464 bits (280), Expect = 8.171e-68
Identity = 52/63 (82.54%), Postives = 57/63 (90.48%), Query Frame = -1 
Query: 3881 SKDLLYRHTQDMDIKIKILQSAQQRDMDRGLTFKGDQRKVYDKLMGDLNKQKYVPTPAETKEL 4069
            +K L+YRH QD DIK+KILQSAQQRDMDRGLTFKGDQRKVYDKLMGDL   KYVP+PAETKE+
Sbjct:  146 TKGLIYRHNQDTDIKLKILQSAQQRDMDRGLTFKGDQRKVYDKLMGDLKSSKYVPSPAETKEM 208

HSP 2 Score: 109.383 bits (272), Expect = 8.171e-68
Identity = 54/77 (70.13%), Postives = 63/77 (81.82%), Query Frame = -3 
Query: 3516 ASKALRKNNPIDRWRHEATIKKLKENLKEIDPYNDVLDKHNKWLMDKPERHSQSMQHLLDLKAKGGNFIKKMMPFIK 3746
            A K L KNNPIDRWRH+A IKK++ENLKE+ PY+DVL+KHNKWL  KPER +QSM HLLDLKA+ GNF+KK   F K
Sbjct:  255 AKKGLEKNNPIDRWRHQANIKKIEENLKELAPYDDVLEKHNKWLDKKPERRTQSMAHLLDLKAR-GNFVKKNAAFHK 330

HSP 3 Score: 78.9518 bits (193), Expect = 8.171e-68
Identity = 38/44 (86.36%), Postives = 42/44 (95.45%), Query Frame = -2 
Query: 3748 EHDKQVKKQLDNEMNQARKMKKFKPTNNIKTMFDIREYYRH*LD 3879
            E+DK+VKKQLD+EMN ARKMKKFKPTNNIKTMFDIREYYRH L+
Sbjct:  210 EYDKKVKKQLDDEMNNARKMKKFKPTNNIKTMFDIREYYRHELN 253

HSP 4 Score: 230.335 bits (586), Expect = 9.499e-65
Identity = 122/195 (62.56%), Postives = 151/195 (77.44%), Query Frame = -2 
Query: 2971 RRKFYQKNDAIHQKQSKNIGKTRSIRTYRLCSQSKRCRIPMPVRHREGRWGQVDDFTMVDVDNALRRMYHSKYSDNKRWYYKNTKWDTRKYPSQFRAPAINGWFGIGDEHQVWDFKTKKWISQIKK*QPNQYLHRNWDNYAYENQYYKSSEHNFYWSLNSEDWKKYKEVIYXXXXXXXXXXXPRTKGYWGPGFKK 3555
            R  F +KN A H+++ KN  KTRSIRT+RLC  +      MP+RH E RWGQVDDFTMVDVDNALRRM HSKYSD KRWYYK+ K+D R+YPS    P +NG FGIGD+HQVWDFKTKKWI QI+  +P+ Y H ++  Y +E +YY+SSEHN YWS++ +DWK++KEV+YKVGPPPA G+AP +K YWGPG+ K
Sbjct:  318 RGNFVKKNAAFHKERRKNTRKTRSIRTHRLCKSAH----TMPIRHCEDRWGQVDDFTMVDVDNALRRMQHSKYSDKKRWYYKDIKYDNRQYPSSHNEPIVNGCFGIGDDHQVWDFKTKKWIKQIQNFKPHTYHHTDYTKYFHEYRYYQSSEHNLYWSVDGKDWKRFKEVVYKVGPPPAWGRAPESKKYWGPGYDK 508
[Close]
BLAST of SMED30033573 vs. Planmine SMEST
Match: SMESG000055972.1 (SMESG000055972.1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.038e-12
Identity = 53/178 (29.78%), Postives = 84/178 (47.19%), Query Frame = -2 
Query:   79 MSVRALNTALGIHYSWGFSAGDIDREFDYASKEATRVTDGLILSIGTPPK--TLPQLKDIFRQLLTFDNPDDGMTFLREKDPQTYVLQGQKIESCLVR----ESRKNMSSKYVKFNIPFVNVRDGKTHVFVGSTGIGKTNFALAHFRRAILCSDRQDFGKISTST---DGLVIDDMTF 585
            MSV       GI +  G +  D      Y++K+  RV  GL + I    K  +L Q  D F+  L      + +  L  K+   Y+ +G+ I   L+      +  N+S   +   IP +N+ D K+HVF+G + IGKT FAL+HF + +     Q    I+++    DG+V  D+ F
Sbjct:    1 MSVFIFRARSGIPFVRGIAGPDRQWASLYSTKDDLRVLGGLSIKIVQLNKYSSLVQATDNFKMALEAKTMKEALNILMTKESFHYMTKGKTILETLINIFGNSTTHNLSENVL---IPKMNLLDWKSHVFLGDSVIGKTYFALSHFNKPVHVKHLQMLNIITSNVDECDGIVFHDLYF 175
The following BLAST results are available for this feature:
BLAST of SMED30033573 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033573 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033573 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033573 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033573 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033573 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033573 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033573 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
Q91S732.608e-2536.94PASVE_E1 OS=Planaria asexual strain-specific virus... [more]
A0A2Z4QAE61.726e-1332.94Putative viral replication protein OS=CRESS virus ... [more]
Q77I172.506e-1325.98Rep OS=Planaria asexual strain-specific virus-like... [more]
Q910M22.506e-1325.98Rep OS=Planaria asexual strain-specific virus-like... [more]
A0A5Q2F4S99.964e-1028.31Replication associated protein OS=Antarctic virus ... [more]
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BLAST of SMED30033573 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033573 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033573 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033573 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033573 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033573 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000059230.12.019e-11958.10SMESG000059230.1[more]
SMESG000070092.15.390e-10196.18SMESG000070092.1[more]
SMESG000059232.19.058e-8479.86SMESG000059232.1[more]
SMESG000059229.18.171e-6882.54SMESG000059229.1[more]
SMESG000055972.12.038e-1229.78SMESG000055972.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033573 ID=SMED30033573|Name=SMED30033573|organism=Schmidtea mediterranea sexual|type=transcript|length=5200bp
TATTATTATATTATTATATTATTAATACATATCAAAGGCCACATCACAAA
TATGTAGATTTGTTGGTAACCACGACTTGAATGTCATATCATCAATAACT
AGACCGTCTGTCGATGTGGATATCTTTCCGAAATCTTGCCGATCAGAACA
CAAGATGGCTCGACGAAAATGTGCTAACGCAAAGTTAGTCTTACCGATAC
CAGTACTACCGACAAATACATGGGTCTTGCCATCTCGTACATTGACAAAC
GGGATATTGAACTTGACATATTTAGAACTCATATTTTTACGACTTTCACG
CACTAGACATGATTCAATTTTCTGCCCCTGTAATACATAGGTTTGAGGAT
CTTTTTCCCTCAAAAATGTCATCCCATCATCTGGATTATCGAATGTTAAA
AGCTGTCTAAATATGTCCTTTAATTGAGGTAGGGTTTTCGGGGGAGTCCC
AATACTGAGAATAAGTCCATCAGTAACACGTGTTGCTTCTTTGCTAGCAT
AATCAAACTCTCTGTCGATGTCACCAGCAGAGAATCCCCAGGAATAGTGG
ATACCGAGCGCGGTATTGAGAGCCCTAACAGACATGTTATTCCCAGTGTG
CAGAAACACCTCACAGTGATGATGTCCACGTTCCGACATTTCTTCCTAGT
AATGCAAATACACTTTATTAAATAAACAAAATAAAATGCAAATGCCATTA
CCTGTAAATAAAAGAACTTGCAACGTGTATTAGCAAACTCAAGTATAGTT
ACTCGGTTATCCATATACATTTGAAATGGGATACTGAACCGGAAATTTCT
AAATCTCCCCAACTGGACAATTGGCGGAGGACGGTCACTGTTCTCATCAT
AATGATAATTATTACTATTACTCATTAAAATAAATAATGAATTCCAGACA
ACTGAGTTAGTATTTATATAATATTTGAATCAATAATTCAAATTCAAATT
TCAAATTCATATAATCAATTATATTATTATGTCATTAAACATGGATACTA
CTACTACTCCTCAACTGTCTGGAATGCAAGAAGCTGCTTTACAGAGAAAA
AGCGATTCCATCGTGCGAGAAATGAACGGATATAAAAAATTGGAATTCGA
TGACTTTACGCTGAATCGTATGGCAACAATTCCGTTCGCGTTACCGATGA
TCTATTTCACAATAGATGACAAAAAGAAGGGCCGTACGCTGAGTACAAAT
GGAGACTTCGCTGCTACTATCTATCCTATGTTTTCGTCCCATTTCTTGCA
CGATGGAATCGCTTCTGGACACGGATATCCCAACTTCATTGTATACGTTA
GTGTAAACGGCAACTGTGTATACTGCGTCAGTTTCGCACCCTGTAATCCA
ATTATCTCACTTATCTTTAGAAATATCGTCGGCATAGGATTATATTCTTG
AATCCAAGGAAATTGTTCTATGTTATCACTCCACTATCTCTTTTTCTGTC
ATTTTCCAAGTCCAGTGATTCAATTTTTCTACTGGTAGATTCGTTCTGGC
TCGAAACATTTTCGATATACACGGAATGACTGATCGGTAATACATATAAT
CGAATCGTGGACTGAAGAGTATTTTTGAGGCATCGGCCATTGTATCTGTT
TTGACTGCTGGTGGACGTTTATAGAGCATCTGAAATTCTTGAGTTCTTTT
CCATGGTCCATCGAATGACCATTCGAAACCGAATGCTTCTCCTTGTTTGC
ACATCATCTCTTTAGTGTAGGAATCATTTTGACCCTGCATAATGTAGTTG
AGGGATTTAGCGAGATTTACGGTTTTTCCAATTTTGAATGAACCGGTCTT
GGTATCCATTCCGCCGCATCTGAGAGCATTATTTGCGAACATTGTATCAT
AATATTCCCAACCCTGATCATGAGAAGGACCAGTTACGGGATTGTACATG
TGAAGTTTATCTACAAAGCCGAGAATTTTCGCCTCAGGATTGAGACCTTT
CATATCGGTATCTGCCGTTTGGTAGGTAGCAATTGGATTGTAGAATAATA
CTGAGGCTGTTTTCGACTTATAATAGAGGAAAGTTTTGAGATCTTTGAAA
TTTTGAGTTCCCACTATCCATGAAAGCATAAACTCCCAATGGAAAGGAAA
CCAACTGGTATTGGCATCTGCACCAGTATTTGAAAGCACTATGGTTCCAT
TGATGACGAATTCTCTGTGACCTGTTCCACGCTTTTGGACTTTCTGAGGT
TCAATAGGTTCACCCATCATTGATTCACCTAGTAAATCTAAATTATTAGC
CAATAATGAAATTATGATACTGACTTGGGTATTTATATACATGTGGTATC
TAGGGTAACAACTACTTGTATTGATTGATATGTTTGTACATTTTGGTGAT
GAGATTTTTCTTCATTTTTCCAGAGAATCGTTTGAATAGTTTTTTGATTA
GACTATGCCAGAAACGTTTTGATAGTGGTCGTTTGAATGATGAATGTATG
GTTGGTAGTTCTCTTTTGAGGTCTTTATTTGTGGGCAATAAGGCTTTTTG
CTTTGTGGGTTTAGGACGATCAATGTATGGCTGTAGAGGACCTACTGCTT
CTTTCATTTGTTCTAATTTGAGCTTTTTCTTCTTTTCATCAGGTACGAAG
TCTTCGGGTATATTTTTGGCGAAATTATTTTCCCAATCATCTGAACGTGC
TTCGAATTGTCGTAATGTAATTTCTCTTCAGTTTTATGGAAGGGATTTTC
AGGTCTTTTTATCCACCAACTTTGCACATCACCTCTAACTCTTTGACCAC
CAGGCACATAGTTATGTACCCATGATGCTGGTACTTTAAATTGATCTTTA
TAATGGAAGTTTCCACTATCATATACCCAACCGGGTGTATTTTCGAGTGT
TGTATCTTCATATTGGCCGAGTTTACCGGTATCTTTTTTGAGTTGATTCA
TTCTATAGAGAACTGCATCACACCATGCTCGATAGATATCTGGACGATAA
CCACCACAATTTTCAATGTCTTTTTTAAATCCAGGTCCCCAGTAACCTTT
AGTTCTAGGGGCTTTTCCCTAAGCTGGTGGTGGACCGACTTTATATATGA
CCTCTTTGTATTTTTTCCAATCTTCACTATTTAGACTCCAATAGAAGTTA
TGTTCTGACGATTTGTAGTATTGATTTTCATAGGCATAATTGTCCCAATT
TCTGTGGAGATATTGGTTGGGTTGTCATTTTTTGATTTGACTTATCCATT
TTTTGGTTTTAAAGTCCCAGACTTGATGTTCATCTCCAATGCCAAACCAT
CCGTTTATTGCGGGAGCTCTGAATTGAGAGGGATATTTTCTGGTGTCCCA
TTTCGTATTCTTATAATACCATCGCTTGTTATCGGAATATTTGCTGTGAT
ACATTCTTCTGAGAGCATTATCTACATCGACCATTGTGAAATCATCTACT
TGTCCCCAACGGCCTTCACGATGTCTAACGGGCATTGGAATCCGACAACG
CTTTGACTGACTGCAAAGTCTATAAGTCCTAATAGACCGAGTTTTCCCAA
TGTTTTTGGACTGTTTTTGATGAATGGCATCATTTTTTTGATAAAATTTC
CTCCTTTAGCTTTCAAATCCAAGAGATGTTGCATCGATTGACTATGACGT
TCTGGTTTGTCCATTAACCATTTATTATGCTTATCTAATACATCATTATA
GGGATCAATTTCTTTAAGATTCTCTTTGAGTTTTTTAATTGTAGCTTCAT
GTCTCCAACGATCTATGGGATTATTTTTTCTCAAGGCTTTACTTGCTGTC
TAGCTAATGCCTGTAATATTCTCTAATATCGAACATTGTTTTGATATTGT
TTGTTGGTTTGAATTTCTTCATTTTTCTTGCTTGATTCATTTCATTATCA
AGTTGTTTTTTAACTTGTTTATCATGCTCTGAGTTCTTTAGTTTCTGCCG
GAGTCGGTACATATTTTTGTTTATTGAGATCTCCCATCAATTTATCATAT
ACTTTTCTTTGATCTCCTTTAAAGGTCAATCCACGATCCATGTCTCTTTG
TTGAGCAGATTGTAGGATTTTAATTTTGATATCCATATCTTGAGTATGTC
TGTAAAGGAGATCTTTAGAAACCTGTTGATTGTCATACTGGAATGTCAAT
CCACCAAAATCTATAGGTTGATTGAACTTAATAACAAGCATGCGGTTTTG
GAGGGCCTCTTGGTGGTTTGATACAAACTGCCAAATCCACTGATTACTTG
TGACTATCACTGGTATTCTCTTCCAATTTACCGCAGGCTTGTTTTTAATT
TCAATTCTAATCATATTCGAGCCTTCGAGCATCAACTTGAAATAATCTAC
ATTCTCCATATTCAACATCAACTCTTCGGCTACTACTACATCTTTGCCGT
CAACATCCTGTGTCCAGAATGTGTTCTTATTGTTCATATTATGGATACAT
CCGACTTTCATGTCGACTGTGAGCAGTTGGACGAAGAAAGTTTTTCTGGT
GTTGGAAGGTCCTATACTATAAAATAAGTTATCATATAGAATATTACCTT
GAAGATATATGGTATTAATTTTTCCGCCCCTAAGTTCCATAATAGTTTGA
ATAATTCTGATCTTAGAATATCTGGTATCGATTGCGTTACTAGATAAAAT
ACCAAAAGGAAGTAATCTTCAACGGTTTTATTAGAAGGAAAAATTAATTT
CAAAGCCTTATCTAGTTCGTCTTGATCTACTTTATCTGTTTTTCTAGTTA
CGGGTTTAGTAGTAGACATAAAAAGTCTTTAATAAAATAATGATTTTTAA
AAAGAGTTGATTATATAACATTTATTTGATACGATAAAACAACAATAATA
TATAAAAATTACGGAATAACTAAATGTTTAATAGGTTTCCGTGGCTTTTT
CTTGGTTGGCGATACTGGTGTTGTCGTTATAATTAACGGGGCTGGTTGCT
GCTGCAAATCAGATTTCGGTCTACCGAGATGGCATTTGCATGTTCCATAG
AGTAATATTGGTTGCCGACTATCCGACGTAGACGGTTGTGTCAAATCCAA
GCCACTACCATTACCACTTGTAGACGGTAATTAATTTGCTGGAAGCTTAT
ATACGAGCATAGGCCAATTATCTCACGAAGAAAAAGAAAAGGAGAGCTTC
ATTGGATTCATTGGAGGTTGACAGTGGAGAAACTATCACAATAAATCGAC
TCACGTATTGAAAGAAGAAAACTGTCATTCATTATAAATGTGGTTCTAAA
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protein sequence of SMED30033573-orf-1

>SMED30033573-orf-1 ID=SMED30033573-orf-1|Name=SMED30033573-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=113bp
MNQLKKDTGKLGQYEDTTLENTPGWVYDSGNFHYKDQFKVPASWVHNYVP
GGQRVRGDVQSWWIKRPENPFHKTEEKLHYDNSKHVQMIGKIISPKIYPK
TSYLMKRRKSSN*
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protein sequence of SMED30033573-orf-2

>SMED30033573-orf-2 ID=SMED30033573-orf-2|Name=SMED30033573-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=141bp
MSLNMDTTTTPQLSGMQEAALQRKSDSIVREMNGYKKLEFDDFTLNRMAT
IPFALPMIYFTIDDKKKGRTLSTNGDFAATIYPMFSSHFLHDGIASGHGY
PNFIVYVSVNGNCVYCVSFAPCNPIISLIFRNIVGIGLYS*
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protein sequence of SMED30033573-orf-3

>SMED30033573-orf-3 ID=SMED30033573-orf-3|Name=SMED30033573-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=206bp
MSERGHHHCEVFLHTGNNMSVRALNTALGIHYSWGFSAGDIDREFDYASK
EATRVTDGLILSIGTPPKTLPQLKDIFRQLLTFDNPDDGMTFLREKDPQT
YVLQGQKIESCLVRESRKNMSSKYVKFNIPFVNVRDGKTHVFVGSTGIGK
TNFALAHFRRAILCSDRQDFGKISTSTDGLVIDDMTFKSWLPTNLHICDV
AFDMY*
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protein sequence of SMED30033573-orf-4

>SMED30033573-orf-4 ID=SMED30033573-orf-4|Name=SMED30033573-orf-4|organism=Schmidtea mediterranea sexual|type=polypeptide|length=223bp
MELRGGKINTIYLQGNILYDNLFYSIGPSNTRKTFFVQLLTVDMKVGCIH
NMNNKNTFWTQDVDGKDVVVAEELMLNMENVDYFKLMLEGSNMIRIEIKN
KPAVNWKRIPVIVTSNQWIWQFVSNHQEALQNRMLVIKFNQPIDFGGLTF
QYDNQQVSKDLLYRHTQDMDIKIKILQSAQQRDMDRGLTFKGDQRKVYDK
LMGDLNKQKYVPTPAETKELRA*
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protein sequence of SMED30033573-orf-5

>SMED30033573-orf-5 ID=SMED30033573-orf-5|Name=SMED30033573-orf-5|organism=Schmidtea mediterranea sexual|type=polypeptide|length=289bp
MYINTQVSIIISLLANNLDLLGESMMGEPIEPQKVQKRGTGHREFVINGT
IVLSNTGADANTSWFPFHWEFMLSWIVGTQNFKDLKTFLYYKSKTASVLF
YNPIATYQTADTDMKGLNPEAKILGFVDKLHMYNPVTGPSHDQGWEYYDT
MFANNALRCGGMDTKTGSFKIGKTVNLAKSLNYIMQGQNDSYTKEMMCKQ
GEAFGFEWSFDGPWKRTQEFQMLYKRPPAVKTDTMADASKILFSPRFDYM
YYRSVIPCISKMFRARTNLPVEKLNHWTWKMTEKEIVE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR001257Parvovirus_NS1_helicase
IPR027417P-loop_NTPase
IPR014015Helicase_SF3_DNA-vir
Vocabulary: biological process
TermDefinition
GO:0019079viral genome replication