SMED30033559

Overview
NameSMED30033559
Smed IDSMED30033559
Length (bp)1556
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30033559 (SMED30033559) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30033559 (SMED30033559) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30033559 (SMED30033559) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30033559 (SMED30033559) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30033559

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30033559h1SMcG0011178 SmedASXL_010310SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30033559h1SMcG0011178 dd_Smed_v4_18869_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30033559h1SMcG0011178 dd_Smed_v6_18869_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
whole organismSMED30033559h1SMcG0011178 Contig17730uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
whole organismSMED30033559h1SMcG0011178 Contig17730newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
zeta neoblastSMED30033559h1SMcG0011178 dd_Smed_v4_18869_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30033559 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000359:167365..175303 +7483
Homology
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BLAST of SMED30033559 vs. Planmine SMEST
Match: SMESG000076700.1 (SMESG000076700.1)

HSP 1 Score: 969.148 bits (2504), Expect = 0.000e+0
Identity = 480/485 (98.97%), Postives = 484/485 (99.79%), Query Frame = 3 
Query:   39 MNWKNVLHENLLLLKYPFNNSLSLDERINMFKSPNNRALNEIVMFCIKEVDSDFFAELNNYNALSDKKCKISFIRKIFDWSTDMITKTFKDSKFNSSIFISAKGLQVYEMLAKLTSCVIKKIIKKSQSGNSHNLRYPVSLNDLQTINLSPEILMASFNDDILIEQKVYLTQLDILTKRQKLFSDKLKAKKENVDRVNKNVNYLIAELNLDSDITLDTFCENTVHMLDWDLMTRSTESETQVNILQDKIIKFLNLCQLVQDKYEERPEEVSIKTPVQNDTVYKIDSSLFLENGSLSFERVVKKVANELLFNEVEYKNELLRKENERVMNGILLVESEISKNLVMAKEMKESLMQLQGEIKEHEIVIETEKLQNFWKPITAKEFIMTTYENELNFEKENNSDSYNGNQDEFYLDYESKSYVDNEDISNPNPDDQHYNDSDQENCAELEFINRAGDMFLQNIGNPSDFDSNFLNTSDSSIVEFMENNS 1493
            MNWKNVLHENLLLLKYPFNNSLSLDERINMFKSPNN ALNEIVMFCIKEVDSDFFAELNNYNALSDKKCKISFIRKIFDWSTDMITKTFKDSKFNSSIFISAKG+QVYEMLAKLTSCV+KKIIKKSQSGNSHNLRYPVSLNDLQTINLSPEILMASFNDDILIEQKVYLTQLDILTKRQKLFSDKLKAKKENVDRVNKNVNYLIAELNLDSDITLDTFCENTVHMLDWDLMTRSTESETQVNILQDKIIKFLNLCQLVQDKYEERPEEVSIKTPVQNDTVYKIDSSLFLENGSLSFERVVKKVANELLFNEVEYKNELLRKENERVMNGILLVESEISKNLVMAKEMKESLMQLQGEIKEHEI+IETEKLQNFWKPITAKEFIMTTYENEL+FEKENNSDSYNGNQDEFYLDYESKSYVDNEDISNPNPDDQHYNDSDQENCAELEFINRAGDMFLQNIGNPSDFDSNFLNTSDSSIVEFMENNS
Sbjct:    1 MNWKNVLHENLLLLKYPFNNSLSLDERINMFKSPNNSALNEIVMFCIKEVDSDFFAELNNYNALSDKKCKISFIRKIFDWSTDMITKTFKDSKFNSSIFISAKGVQVYEMLAKLTSCVLKKIIKKSQSGNSHNLRYPVSLNDLQTINLSPEILMASFNDDILIEQKVYLTQLDILTKRQKLFSDKLKAKKENVDRVNKNVNYLIAELNLDSDITLDTFCENTVHMLDWDLMTRSTESETQVNILQDKIIKFLNLCQLVQDKYEERPEEVSIKTPVQNDTVYKIDSSLFLENGSLSFERVVKKVANELLFNEVEYKNELLRKENERVMNGILLVESEISKNLVMAKEMKESLMQLQGEIKEHEIIIETEKLQNFWKPITAKEFIMTTYENELDFEKENNSDSYNGNQDEFYLDYESKSYVDNEDISNPNPDDQHYNDSDQENCAELEFINRAGDMFLQNIGNPSDFDSNFLNTSDSSIVEFMENNS 485
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BLAST of SMED30033559 vs. Planmine SMEST
Match: SMESG000076700.1 (SMESG000076700.1)

HSP 1 Score: 664.455 bits (1713), Expect = 0.000e+0
Identity = 330/332 (99.40%), Postives = 332/332 (100.00%), Query Frame = 3 
Query:  498 MASFNDDILIEQKVYLTQLDILTKRQKLFSDKLKAKKENVDRVNKNVNYLIAELNLDSDITLDTFCENTVHMLDWDLMTRSTESETQVNILQDKIIKFLNLCQLVQDKYEERPEEVSIKTPVQNDTVYKIDSSLFLENGSLSFERVVKKVANELLFNEVEYKNELLRKENERVMNGILLVESEISKNLVMAKEMKESLMQLQGEIKEHEIVIETEKLQNFWKPITAKEFIMTTYENELNFEKENNSDSYNGNQDEFYLDYESKSYVDNEDISNPNPDDQHYNDSDQENCAELEFINRAGDMFLQNIGNPSDFDSNFLNTSDSSIVEFMENNS 1493
            MASFNDDILIEQKVYLTQLDILTKRQKLFSDKLKAKKENVDRVNKNVNYLIAELNLDSDITLDTFCENTVHMLDWDLMTRSTESETQVNILQDKIIKFLNLCQLVQDKYEERPEEVSIKTPVQNDTVYKIDSSLFLENGSLSFERVVKKVANELLFNEVEYKNELLRKENERVMNGILLVESEISKNLVMAKEMKESLMQLQGEIKEHEI+IETEKLQNFWKPITAKEFIMTTYENEL+FEKENNSDSYNGNQDEFYLDYESKSYVDNEDISNPNPDDQHYNDSDQENCAELEFINRAGDMFLQNIGNPSDFDSNFLNTSDSSIVEFMENNS
Sbjct:    1 MASFNDDILIEQKVYLTQLDILTKRQKLFSDKLKAKKENVDRVNKNVNYLIAELNLDSDITLDTFCENTVHMLDWDLMTRSTESETQVNILQDKIIKFLNLCQLVQDKYEERPEEVSIKTPVQNDTVYKIDSSLFLENGSLSFERVVKKVANELLFNEVEYKNELLRKENERVMNGILLVESEISKNLVMAKEMKESLMQLQGEIKEHEIIIETEKLQNFWKPITAKEFIMTTYENELDFEKENNSDSYNGNQDEFYLDYESKSYVDNEDISNPNPDDQHYNDSDQENCAELEFINRAGDMFLQNIGNPSDFDSNFLNTSDSSIVEFMENNS 332
The following BLAST results are available for this feature:
BLAST of SMED30033559 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033559 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033559 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033559 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033559 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033559 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033559 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033559 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033559 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033559 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033559 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033559 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033559 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30033559 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000076700.10.000e+098.97SMESG000076700.1[more]
SMESG000076700.10.000e+099.40SMESG000076700.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30033559 ID=SMED30033559|Name=SMED30033559|organism=Schmidtea mediterranea sexual|type=transcript|length=1556bp
CAAAATCTGAAAATTTTAATTTAATTTAAGATATACAAATGAATTGGAAA
AACGTCTTGCACGAAAATTTATTGCTTTTGAAATACCCATTTAATAATAG
TTTGTCTTTGGATGAACGTATTAATATGTTTAAAAGTCCTAACAATAGAG
CTCTTAATGAAATTGTTATGTTCTGCATTAAAGAAGTAGATTCAGATTTC
TTTGCAGAGTTGAATAATTATAATGCTTTATCAGATAAGAAATGCAAAAT
TAGCTTCATTAGAAAAATCTTTGATTGGTCCACCGATATGATAACAAAAA
CATTTAAGGACTCTAAATTCAATTCCAGTATTTTCATATCGGCCAAAGGA
TTACAAGTATATGAAATGCTGGCTAAATTAACCAGTTGTGTTATAAAGAA
AATAATTAAGAAATCTCAATCAGGAAATTCTCATAATCTCCGTTATCCAG
TATCGTTGAATGACTTGCAAACGATCAATTTATCCCCAGAAATCCTCATG
GCAAGTTTCAATGATGATATCCTCATAGAGCAGAAAGTGTATCTTACACA
ACTTGACATATTGACTAAAAGACAAAAACTATTTAGCGATAAATTAAAGG
CCAAAAAAGAAAACGTTGATAGAGTCAATAAAAATGTTAATTATTTGATA
GCAGAATTGAATTTAGACAGTGATATTACATTGGATACATTTTGTGAAAA
TACGGTTCACATGTTGGATTGGGATCTTATGACACGCTCCACAGAGAGTG
AAACCCAAGTAAACATCCTGCAAGATAAAATTATCAAATTCCTCAACCTC
TGTCAATTAGTGCAAGATAAATACGAAGAACGGCCAGAGGAAGTGTCAAT
TAAAACTCCAGTACAGAATGATACTGTATACAAGATTGATTCTAGCCTTT
TTTTGGAAAATGGATCTTTGAGTTTTGAAAGAGTCGTAAAGAAAGTAGCC
AATGAATTATTATTCAATGAGGTTGAGTACAAAAATGAATTGCTGAGAAA
AGAAAATGAAAGAGTTATGAATGGAATTTTACTTGTTGAATCAGAGATTT
CGAAAAATTTAGTTATGGCTAAGGAAATGAAGGAGAGTTTAATGCAGTTG
CAAGGGGAAATAAAAGAACACGAAATTGTCATTGAGACTGAAAAATTACA
AAACTTTTGGAAACCAATAACTGCCAAGGAATTTATTATGACTACTTATG
AAAATGAATTGAATTTTGAAAAAGAAAATAATTCTGATAGTTATAATGGA
AATCAAGACGAATTCTATTTAGATTACGAAAGTAAAAGTTATGTCGATAA
TGAAGATATAAGTAATCCTAATCCCGATGATCAACATTATAATGATAGTG
ACCAAGAAAATTGTGCTGAATTAGAATTTATAAACAGAGCCGGGGACATG
TTCTTACAAAATATCGGTAATCCCTCAGATTTCGATTCCAATTTCCTCAA
TACGAGTGACAGCTCGATTGTGGAGTTCATGGAAAACAATTCCTAATTAA
CTTTTTATAATTGAAATATTTCATAATTGTAGAAAACGAAGTTGTAAAAA
TTTGTG
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protein sequence of SMED30033559-orf-1

>SMED30033559-orf-1 ID=SMED30033559-orf-1|Name=SMED30033559-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=486bp
MNWKNVLHENLLLLKYPFNNSLSLDERINMFKSPNNRALNEIVMFCIKEV
DSDFFAELNNYNALSDKKCKISFIRKIFDWSTDMITKTFKDSKFNSSIFI
SAKGLQVYEMLAKLTSCVIKKIIKKSQSGNSHNLRYPVSLNDLQTINLSP
EILMASFNDDILIEQKVYLTQLDILTKRQKLFSDKLKAKKENVDRVNKNV
NYLIAELNLDSDITLDTFCENTVHMLDWDLMTRSTESETQVNILQDKIIK
FLNLCQLVQDKYEERPEEVSIKTPVQNDTVYKIDSSLFLENGSLSFERVV
KKVANELLFNEVEYKNELLRKENERVMNGILLVESEISKNLVMAKEMKES
LMQLQGEIKEHEIVIETEKLQNFWKPITAKEFIMTTYENELNFEKENNSD
SYNGNQDEFYLDYESKSYVDNEDISNPNPDDQHYNDSDQENCAELEFINR
AGDMFLQNIGNPSDFDSNFLNTSDSSIVEFMENNS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000136whole organism
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR028163HAUS_6_N
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 344..371
IPR028163HAUS augmin-like complex subunit 6, N-terminalPFAMPF14661HAUS6_Ncoord: 11..208
e-value: 2.7E-9
score: 36.9